##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939655_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1511181 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.53329945254738 31.0 31.0 34.0 30.0 34.0 2 31.711415773491066 31.0 31.0 34.0 30.0 34.0 3 31.751591635945662 31.0 31.0 34.0 30.0 34.0 4 35.52754104240326 37.0 35.0 37.0 33.0 37.0 5 35.28379194815181 37.0 35.0 37.0 33.0 37.0 6 35.328916258211294 37.0 35.0 37.0 32.0 37.0 7 35.267122204421575 37.0 35.0 37.0 32.0 37.0 8 35.239083207107555 37.0 35.0 37.0 32.0 37.0 9 36.81918578912784 39.0 37.0 39.0 32.0 39.0 10-11 36.70341838601729 39.0 35.0 39.0 32.0 39.0 12-13 36.64684673775014 39.0 35.0 39.0 32.0 39.0 14-15 37.61026045192469 39.0 36.0 41.0 32.0 41.0 16-17 37.600350983767 39.0 36.0 41.0 32.0 41.0 18-19 37.66021906045669 39.0 36.5 41.0 32.0 41.0 20-21 37.61552686276495 39.0 36.5 41.0 32.0 41.0 22-23 37.476692401505844 39.0 36.0 41.0 32.0 41.0 24-25 37.32955218468204 39.0 36.0 41.0 31.5 41.0 26-27 37.02519651848455 39.0 36.0 40.0 31.0 41.0 28-29 36.81869974543089 39.0 36.0 40.0 30.5 41.0 30-31 36.5678644715623 38.0 35.0 40.0 30.0 41.0 32-33 36.36189311538459 38.0 35.0 40.0 30.0 41.0 34-35 36.30734207219386 38.0 35.0 40.0 30.0 41.0 36-37 36.144376153485254 38.0 35.0 40.0 30.0 41.0 38-39 36.11507225143778 38.0 35.0 40.0 30.0 41.0 40-41 35.99146958570813 38.0 35.0 40.0 30.0 41.0 42-43 35.79109683088922 38.0 35.0 40.0 29.0 41.0 44-45 35.519643907645744 38.0 35.0 40.0 27.5 41.0 46-47 35.112850148327695 38.0 34.0 40.0 26.5 41.0 48-49 34.742471616570086 38.0 34.0 40.0 24.5 41.0 50-51 34.46257397360078 38.0 33.0 40.0 24.0 41.0 52-53 34.17549419956974 38.0 33.0 40.0 23.5 41.0 54-55 33.90502494406692 38.0 33.0 40.0 23.0 41.0 56-57 31.949891839561243 36.0 30.0 39.5 14.5 41.0 58-59 27.065396865100873 32.5 17.0 38.0 4.0 40.0 60-61 27.765776898994893 32.5 18.5 38.0 5.5 40.0 62-63 30.74676395481415 34.5 29.0 38.0 2.0 40.0 64-65 30.925825893787707 35.0 30.5 39.0 2.0 40.0 66-67 24.36362222658967 26.5 16.5 35.5 2.0 38.5 68-69 17.077826878448047 13.0 7.0 28.0 2.0 35.0 70-71 15.972290877135169 12.5 7.0 26.5 2.0 33.5 72-73 17.080225664563013 13.0 7.0 28.5 2.0 34.0 74-75 18.04969391489173 17.0 7.5 29.0 2.0 34.5 76-77 19.194472402710197 21.0 8.0 30.0 2.0 34.0 78-79 22.212310437995185 27.5 10.0 33.5 2.0 35.5 80-81 25.730756937785745 31.5 22.0 34.5 2.0 36.0 82-83 26.99123566270354 33.0 26.0 35.0 2.0 36.0 84-85 27.033845714047487 33.0 26.0 35.0 2.0 36.0 86-87 26.78952223459665 33.0 26.0 35.0 2.0 36.0 88-89 26.336077875515905 33.0 25.0 35.0 2.0 35.0 90-91 25.03156008446374 32.5 12.5 35.0 2.0 35.0 92-93 22.66231245628419 31.5 2.0 34.0 2.0 35.0 94-95 15.55417782515794 4.5 2.0 32.0 2.0 34.5 96-97 13.043458394461021 2.0 2.0 30.0 2.0 34.0 98-99 12.739332680863509 2.0 2.0 29.5 2.0 34.0 100-101 12.453784159541446 2.0 2.0 30.0 2.0 34.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.4275228482358244 1101 2 0.4500495510313307 1101 3 0.47671235732140005 1101 4 0.3802686553395347 1101 5 0.4295792934892333 1101 6 0.4483259576290024 1101 7 0.39513555942355794 1101 8 0.42570550029764576 1101 9 0.6421436883276499 1101 10-11 0.6673805145391967 1101 12-13 0.5223290085329495 1101 14-15 0.6264802997044967 1101 16-17 0.7593210887471287 1101 18-19 0.7865823473505316 1101 20-21 0.6170999798399421 1101 22-23 0.7698604568322267 1101 24-25 0.683253291140808 1101 26-27 0.6470524954935755 1101 28-29 0.6437935958413163 1101 30-31 0.8092127307161476 1101 32-33 0.9648992982768405 1101 34-35 0.8750065250818793 1101 36-37 0.5949165191031582 1101 38-39 0.7854467768001143 1101 40-41 0.9281078005226036 1101 42-43 0.8413151804664949 1101 44-45 0.5862045023447635 1101 46-47 0.7768489914689951 1101 48-49 0.8187277271984144 1101 50-51 0.7409472882859589 1101 52-53 0.6022278484063008 1101 54-55 0.4504138915049509 1101 56-57 -1.4324317307223389 1101 58-59 -8.985345026965057 1101 60-61 -9.676656969558408 1101 62-63 -3.467276949389305 1101 64-65 -4.5572132888923385 1101 66-67 -9.52562610154683 1101 68-69 -4.110988202996786 1101 70-71 -4.208090609875645 1101 72-73 -4.338403098499732 1101 74-75 -6.370135237094527 1101 76-77 -7.514104439362647 1101 78-79 -10.495610858453244 1101 80-81 -7.478797667680276 1101 82-83 -3.4055721026076213 1101 84-85 -2.516073598523761 1101 86-87 -2.2083776987588557 1101 88-89 -2.6214757297102693 1101 90-91 -9.120479055527195 1101 92-93 -16.342549851912185 1101 94-95 -10.532237117374894 1101 96-97 -8.796268832214189 1101 98-99 -8.940320694007386 1101 100-101 -8.85114378619304 1102 1 0.2505495075826616 1102 2 0.24073417805488617 1102 3 0.26329698836193316 1102 4 0.24341756472989573 1102 5 0.172125120518146 1102 6 0.36594911149266807 1102 7 0.32593440253376826 1102 8 0.2844370724090979 1102 9 0.39774938172507746 1102 10-11 0.236038709844685 1102 12-13 0.27054245737673455 1102 14-15 0.47723549164912527 1102 16-17 0.3225831430298953 1102 18-19 0.3919644289487465 1102 20-21 0.44192591077469245 1102 22-23 0.6065793219019184 1102 24-25 0.5467161943450165 1102 26-27 0.5142689302913297 1102 28-29 0.45093344025035265 1102 30-31 0.4550869591215374 1102 32-33 0.5483500166464879 1102 34-35 0.4368338485691581 1102 36-37 0.43355784309925127 1102 38-39 0.43092332434105174 1102 40-41 0.42538529339247333 1102 42-43 0.33560437613351013 1102 44-45 0.4673615260315529 1102 46-47 0.6058375163006104 1102 48-49 0.5421129018700199 1102 50-51 0.6188840342656334 1102 52-53 0.49314546017872374 1102 54-55 0.3247705054599379 1102 56-57 -2.541237951682934 1102 58-59 -10.429866537884543 1102 60-61 -10.649580526458909 1102 62-63 -4.497142179763021 1102 64-65 -5.545815782096948 1102 66-67 -6.657575139480496 1102 68-69 -4.0937463273356585 1102 70-71 -4.034040625274063 1102 72-73 -4.150725514578358 1102 74-75 -4.932812151519496 1102 76-77 -7.06117974431826 1102 78-79 -9.491442239088729 1102 80-81 -5.561703750724607 1102 82-83 -3.20035170439116 1102 84-85 -2.8626511329024 1102 86-87 -2.7838352995515407 1102 88-89 -2.6760584326011525 1102 90-91 -8.666929050840718 1102 92-93 -12.907274086343046 1102 94-95 -10.133081352341353 1102 96-97 -8.979757377130701 1102 98-99 -8.745870819203214 1102 100-101 -8.70399651037391 1103 1 0.20879509949988062 1103 2 0.3025064331849272 1103 3 0.2797877782218201 1103 4 0.27275340242813684 1103 5 0.3721524032559529 1103 6 0.3083337803669082 1103 7 0.3376704120049183 1103 8 0.3231071522086424 1103 9 0.43150019675993434 1103 10-11 0.4377980032119311 1103 12-13 0.4698007510335458 1103 14-15 0.662380385640418 1103 16-17 0.5410144545514868 1103 18-19 0.5417235135094813 1103 20-21 0.4511057640979672 1103 22-23 0.5918449198469489 1103 24-25 0.5723117361289667 1103 26-27 0.694399759391068 1103 28-29 0.6286905726671748 1103 30-31 0.560846226880436 1103 32-33 0.633545536367869 1103 34-35 0.737718616921974 1103 36-37 0.48710834177254725 1103 38-39 0.4548875652407318 1103 40-41 0.6673282675457699 1103 42-43 0.40870635447673465 1103 44-45 0.4361090021320706 1103 46-47 0.5596415375988784 1103 48-49 0.48740879645819035 1103 50-51 0.056672989976981114 1103 52-53 0.20271234425368334 1103 54-55 0.24331946763246748 1103 56-57 -2.6923727461365594 1103 58-59 -10.232454753711679 1103 60-61 -10.048184773110616 1103 62-63 -4.065464015605642 1103 64-65 -5.555723257173138 1103 66-67 -6.3451626757015305 1103 68-69 -4.389737193035643 1103 70-71 -4.09347636506963 1103 72-73 -4.371919818888241 1103 74-75 -4.951523972922953 1103 76-77 -6.4893805905756 1103 78-79 -9.114025697673139 1103 80-81 -4.955987820166563 1103 82-83 -2.8262007195532703 1103 84-85 -2.3530120529905716 1103 86-87 -2.2378023278211856 1103 88-89 -2.2157277303612126 1103 90-91 -4.942726750817275 1103 92-93 -9.938387241744339 1103 94-95 -8.103598759122049 1103 96-97 -7.754707539349724 1103 98-99 -8.19061490318283 1103 100-101 -8.017543128397852 1104 1 0.18415118562727528 1104 2 0.14055355519735357 1104 3 0.11940563398183102 1104 4 0.15672781847587203 1104 5 0.19354883649355514 1104 6 0.14524788694738078 1104 7 0.22468897879836902 1104 8 0.304063299624886 1104 9 0.09331538189987754 1104 10-11 0.20390920519802336 1104 12-13 0.2951443102176583 1104 14-15 0.43207346517719003 1104 16-17 0.22073612864804915 1104 18-19 0.3321496159322521 1104 20-21 0.19718752259092298 1104 22-23 0.19470458288252956 1104 24-25 0.3606892174472591 1104 26-27 0.3488244621091141 1104 28-29 0.3670149438870922 1104 30-31 0.3186819851953189 1104 32-33 0.3922768158346557 1104 34-35 0.26068723330555343 1104 36-37 0.11413651600679486 1104 38-39 0.19879212028577342 1104 40-41 0.07071944567889688 1104 42-43 0.26200735932393115 1104 44-45 0.2632688645634573 1104 46-47 0.09407323884060048 1104 48-49 -0.053799049342380556 1104 50-51 -0.0011675306469172142 1104 52-53 -0.14384046871801814 1104 54-55 0.009923258016328873 1104 56-57 -3.070169412106136 1104 58-59 -10.351231502876722 1104 60-61 -10.293395650224408 1104 62-63 -4.3146288515440965 1104 64-65 -5.5978108878001684 1104 66-67 -6.425496617092001 1104 68-69 -4.286635273410994 1104 70-71 -4.354741607046691 1104 72-73 -4.635944301297721 1104 74-75 -5.417938927401488 1104 76-77 -6.588253993907973 1104 78-79 -9.039741131074317 1104 80-81 -4.990411860302476 1104 82-83 -2.812408037358715 1104 84-85 -2.4980808890615407 1104 86-87 -2.3716927822904026 1104 88-89 -2.107247921516155 1104 90-91 -4.510656533676691 1104 92-93 -6.750590555069071 1104 94-95 -7.187151193179598 1104 96-97 -7.428433052281463 1104 98-99 -7.817418834779508 1104 100-101 -7.889908898577102 1105 1 0.2604847220720572 1105 2 0.35715421064348263 1105 3 0.20800408264953063 1105 4 0.21270711757669858 1105 5 0.17953785200688088 1105 6 0.13576862078355134 1105 7 0.2470441383780937 1105 8 0.31328895095389697 1105 9 0.41954104969737926 1105 10-11 0.36824482490751365 1105 12-13 0.35597296375344456 1105 14-15 0.478662222135128 1105 16-17 0.40665350620477625 1105 18-19 0.38029402817164026 1105 20-21 0.39036483065060423 1105 22-23 0.5452416617007501 1105 24-25 0.5389617134124762 1105 26-27 0.6253414164255062 1105 28-29 0.6665097727794134 1105 30-31 0.754646503821192 1105 32-33 0.7250178322702183 1105 34-35 0.5324120612097758 1105 36-37 0.4704785337268973 1105 38-39 0.5033849778991595 1105 40-41 0.47402240836128584 1105 42-43 0.35079704839873926 1105 44-45 0.49404148404239123 1105 46-47 0.5715754209162895 1105 48-49 0.45484341138025286 1105 50-51 0.40350746358911493 1105 52-53 0.3628811478295262 1105 54-55 0.31881192275069736 1105 56-57 1.4705617067634726 1105 58-59 -8.88098657517342 1105 60-61 -10.10775000284784 1105 62-63 -3.8992118692312623 1105 64-65 -4.6706718360561545 1105 66-67 -6.127862522365216 1105 68-69 -3.6474342836544 1105 70-71 -4.006928829140826 1105 72-73 -2.4224055509271807 1105 74-75 -4.339555680802233 1105 76-77 -6.295280071771021 1105 78-79 -8.918872870806211 1105 80-81 -4.522056898344701 1105 82-83 -2.41799518040407 1105 84-85 -1.9056067857711376 1105 86-87 -1.5972753553281862 1105 88-89 -1.6798960144643473 1105 90-91 -4.324412874047564 1105 92-93 -6.680256491632427 1105 94-95 -7.916738286819465 1105 96-97 -8.042149662406295 1105 98-99 -8.388674104698152 1105 100-101 -8.037832838829399 1106 1 0.28019220531046685 1106 2 0.199949430887159 1106 3 0.14141461541573008 1106 4 0.12191247407586303 1106 5 0.17393713828760582 1106 6 0.25384950896753367 1106 7 0.06491775960223123 1106 8 0.04741770879399354 1106 9 0.1483881047042246 1106 10-11 0.25889032631332753 1106 12-13 0.2736983440432539 1106 14-15 0.27009263450626264 1106 16-17 0.2317048723643964 1106 18-19 0.47465726802456487 1106 20-21 0.46346022752177163 1106 22-23 0.2809352850876792 1106 24-25 0.4427308180192071 1106 26-27 0.4289122656793367 1106 28-29 0.42697495193016266 1106 30-31 0.4345123209937114 1106 32-33 0.31951714738808334 1106 34-35 0.4485455411319137 1106 36-37 0.42867549140839145 1106 38-39 0.35781823793809053 1106 40-41 0.36066225047793665 1106 42-43 0.2062900734149551 1106 44-45 0.20207893628087703 1106 46-47 0.05339315215412199 1106 48-49 0.14554298242776298 1106 50-51 0.23371707356675842 1106 52-53 0.2641914354800221 1106 54-55 0.15294924161739232 1106 56-57 1.3919868022864996 1106 58-59 -8.189493081553636 1106 60-61 -9.86816832164245 1106 62-63 -3.807976558150589 1106 64-65 -5.616117820484096 1106 66-67 -7.423813156782341 1106 68-69 -4.058588400156454 1106 70-71 -4.289075484032324 1106 72-73 -4.502262807073096 1106 74-75 -5.073245122803531 1106 76-77 -6.591406432523671 1106 78-79 -9.037384063222042 1106 80-81 -4.760732913051644 1106 82-83 -2.588396106797262 1106 84-85 -2.0859682413258938 1106 86-87 -1.914030636129855 1106 88-89 -1.7072602095476128 1106 90-91 -4.26136812240745 1106 92-93 -6.291101960596453 1106 94-95 -7.230781524509609 1106 96-97 -7.1167549540218955 1106 98-99 -6.641055421834625 1106 100-101 -7.062583667897238 1107 1 0.002366649526237552 1107 2 0.035681200682798675 1107 3 -0.022935936974896265 1107 4 0.10922741336325714 1107 5 -0.10914171147878449 1107 6 -0.041025497053368554 1107 7 0.042797612998676016 1107 8 0.06109895553723277 1107 9 0.03866138808959363 1107 10-11 -0.027874630708360826 1107 12-13 0.07345488055621274 1107 14-15 0.08800121309110409 1107 16-17 0.05030114772334571 1107 18-19 0.006825975960346398 1107 20-21 0.04714861177371432 1107 22-23 0.03895228374337023 1107 24-25 0.036272029468996436 1107 26-27 -0.026371242296711728 1107 28-29 0.054283804630372856 1107 30-31 0.06095153613048154 1107 32-33 0.08801643144779803 1107 34-35 0.1968202782920585 1107 36-37 0.07298387557275277 1107 38-39 0.00724066898231257 1107 40-41 0.05207206170615564 1107 42-43 -0.10303532254162207 1107 44-45 0.014360267973607677 1107 46-47 0.2735603362536807 1107 48-49 0.1895071897920957 1107 50-51 0.18862203085118523 1107 52-53 0.29099781632522337 1107 54-55 0.3046755991671688 1107 56-57 1.5209933311364843 1107 58-59 -8.694964660694598 1107 60-61 -10.382042894158225 1107 62-63 -4.007455257284892 1107 64-65 -5.0991804685436115 1107 66-67 -6.67484393564656 1107 68-69 -4.071437448415647 1107 70-71 -3.9025366940808865 1107 72-73 -2.442754594559247 1107 74-75 -4.557254257063136 1107 76-77 -6.472183326262368 1107 78-79 -7.157380034689522 1107 80-81 -2.3550080180343222 1107 82-83 -1.2781669183276243 1107 84-85 -1.0357059488281593 1107 86-87 -0.9180858795714499 1107 88-89 -0.6789932667221237 1107 90-91 -0.4892271763153424 1107 92-93 -3.1473956668901586 1107 94-95 -5.638323419058391 1107 96-97 -6.556311202407198 1107 98-99 -6.149805438255274 1107 100-101 -6.840296906443289 1108 1 -0.08065816503435386 1108 2 -0.17630883612326542 1108 3 -0.10528246062782998 1108 4 -0.16998908921903677 1108 5 -0.12175668699931208 1108 6 -0.1843274805041517 1108 7 -0.15592502276953013 1108 8 -0.15838277925063693 1108 9 -0.14339274083740605 1108 10-11 -0.11534462492532782 1108 12-13 -0.2035584312406442 1108 14-15 -0.10062253195489745 1108 16-17 -0.18704604379713174 1108 18-19 -0.23163902507813106 1108 20-21 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11.519574124743098 2316 96-97 13.371020567025683 2316 98-99 13.014755372762936 2316 100-101 11.701848407380426 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 12.0 4 33.0 5 87.0 6 197.0 7 477.0 8 892.0 9 1649.0 10 2790.0 11 4535.0 12 6453.0 13 9103.0 14 11103.0 15 15168.0 16 20277.0 17 27279.0 18 28758.0 19 26623.0 20 26825.0 21 26619.0 22 26488.0 23 22938.0 24 23380.0 25 26865.0 26 37939.0 27 55685.0 28 78406.0 29 105742.0 30 131195.0 31 142458.0 32 142270.0 33 130762.0 34 116354.0 35 104332.0 36 83597.0 37 52744.0 38 19859.0 39 1286.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.39753170911868 19.365877195711267 14.453917448735574 21.782673646434475 2 15.141281523379929 22.248737807408173 39.49251552386039 23.117465145351513 3 20.053852099292207 27.086454522835197 30.56579776280195 22.293895615070646 4 12.25491850413683 17.083526063390156 38.57810546850444 32.08344996396858 5 12.377617048628482 39.69538034384846 33.89395631156422 14.033046295958837 6 29.22972165478523 37.62487749647461 18.106434636221604 15.038966212518554 7 26.59780661614988 34.84427080541643 21.11858208910779 17.439340489325897 8 25.402573132850787 33.94783059833743 20.430545133057255 20.21905113575453 9 26.741866129867965 17.122766895560492 20.94917815933366 35.186188815237884 10-11 24.3487709281681 26.69541239600021 27.71607107288935 21.239745602942335 12-13 27.169445619022476 24.52720752841652 27.69817116546595 20.60517568709506 14-15 23.41196104745191 25.357140021122675 27.998401240891173 23.232497690534235 16-17 20.903915546847134 28.86937435025983 28.429883647293074 21.79682645559996 18-19 20.661800712026363 28.061183975436414 30.956537275506555 20.320478037030664 20-21 22.294847539771872 26.376655079702566 30.04289360440609 21.285603776119473 22-23 21.43936007559663 26.626404491593814 29.816696042194884 22.117539390614677 24-25 21.845629345525122 26.546522223347168 30.480399105070806 21.127449326056905 26-27 22.064416655224154 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29.130727555230663 21.339361156313895 52-53 21.82648057062036 27.876986393821173 28.867008352730757 21.429524682827708 54-55 20.901508120380765 27.28172451597074 29.35909436621164 22.457672997436852 56-57 21.006273364598687 27.899089884736032 29.164708007597103 21.929928743068178 58-59 20.718610696742335 26.979933944500207 30.265902629648878 22.035552729108588 60-61 21.62895087884886 26.93826157838601 29.586032603761236 21.846754939003887 62-63 22.433446991693177 26.963204140009893 28.86389618210485 21.73945268619208 64-65 21.56666908490776 27.44405498616468 29.408991815413337 21.580284113514224 66-67 21.181867943039435 27.11582765864808 29.580930494366235 22.12137390394625 68-69 21.461106800204107 27.234811660295684 29.58038032418778 21.72370121531243 70-71 21.400779534314516 27.229454186449114 29.175193071380857 22.194573207855512 72-73 20.606624223991858 27.022089241063952 29.730757325506683 22.64052920943751 74-75 21.048146455005305 27.605966295253147 29.205108449550583 22.140778800190965 76-77 19.621106292848857 27.425080458790436 30.09264463539274 22.861168612967965 78-79 20.096198383869822 27.398539358149797 29.89483498901463 22.61042726896575 80-81 21.070434956480515 28.25101730847918 28.493079124287092 22.18546861075321 82-83 21.302307668383428 27.98334942452218 28.08254349749434 22.631799409600053 84-85 21.315998529625368 27.492134455238443 27.94902921856735 23.24283779656884 86-87 21.526361936903406 27.6346551524476 28.16189389051566 22.67708902013334 88-89 21.84381985444468 27.13068877392339 28.056125750904258 22.96936562072767 90-91 20.694609682134463 27.364920330465797 28.902390931792425 23.038079055607316 92-93 19.69509768950196 28.187770561692215 29.39838571361196 22.718746035193863 94-95 19.12573545199323 28.071107083970826 29.277673723314756 23.525483740721185 96-97 18.981469079584723 27.994578631711477 28.72938630504318 24.29456598366062 98-99 19.37484333668779 27.832866964619175 28.523712324046336 24.2685773746467 100-101 20.019651043686167 27.708004994918582 28.491341551103233 23.781002410292018 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1103.0 1 833.0 2 574.0 3 603.0 4 671.0 5 810.0 6 1056.5 7 1500.0 8 1955.0 9 2274.0 10 2483.0 11 2680.5 12 2873.5 13 3065.5 14 3240.0 15 3415.0 16 3641.0 17 3830.5 18 3946.0 19 4132.5 20 4542.0 21 5038.0 22 5594.0 23 6398.0 24 7350.0 25 8474.5 26 9961.0 27 11832.5 28 14041.0 29 16252.0 30 18580.5 31 21347.5 32 24508.5 33 27864.5 34 31304.0 35 34481.5 36 37352.5 37 39912.0 38 41868.5 39 43245.5 40 44724.0 41 46706.5 42 48944.0 43 51548.0 44 54919.5 45 59147.0 46 64312.0 47 70440.5 48 75838.0 49 72792.5 50 63367.5 51 55339.0 52 48886.5 53 44378.5 54 40697.0 55 36697.5 56 32625.5 57 28825.0 58 24967.5 59 21202.5 60 17708.0 61 14190.5 62 10854.0 63 8127.0 64 5898.0 65 4183.5 66 2968.5 67 2074.5 68 1436.0 69 974.0 70 652.5 71 496.0 72 400.0 73 305.0 74 191.5 75 117.5 76 77.5 77 38.0 78 19.5 79 13.0 80 6.5 81 1.5 82 0.5 83 0.5 84 1.5 85 2.0 86 1.5 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02236661260299064 2 0.007940809208162357 3 3.3086705034009823E-4 4 0.0 5 0.138037733401889 6 0.0 7 0.0 8 0.0017866820718365306 9 0.0 10-11 0.0 12-13 0.0 14-15 5.955606906121768E-4 16-17 0.0 18-19 6.617341006801964E-5 20-21 0.0 22-23 1.6543352517004912E-4 24-25 0.0 26-27 2.316069352380688E-4 28-29 0.002977803453060884 30-31 0.012374427682719673 32-33 0.0026800231077547957 34-35 0.004003491309115189 36-37 0.10600980292896749 38-39 0.1053811555333213 40-41 0.1168622421801227 42-43 0.1192444849425714 44-45 0.1148770398780821 46-47 0.10882217285685832 48-49 0.10898760638202835 50-51 0.10756487806556594 52-53 0.08705112094447985 54-55 0.07427965280135206 56-57 0.5452358122554479 58-59 1.5646041076482566 60-61 0.5138696158832066 62-63 0.004863745639999444 64-65 0.12040251961876175 66-67 1.895636591513525 68-69 2.867161511427155 70-71 2.7773311072598186 72-73 2.6222868074704486 74-75 2.0025397354784107 76-77 1.3984095882624252 78-79 0.9239462380747243 80-81 0.05446071648598017 82-83 1.3234682013603928E-4 84-85 9.926011510202948E-5 86-87 3.970404604081179E-4 88-89 1.9852023020405896E-4 90-91 0.24302184847480218 92-93 0.38036476107097694 94-95 1.9930769378386837 96-97 2.424957698647614 98-99 1.397747854161745 100-101 0.9303650588513223 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1511181.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.069654358720385 #Duplication Level Percentage of deduplicated Percentage of total 1 74.88435864274369 38.99202671402119 2 13.795314244943333 14.366344890082622 3 4.915992738847074 7.6792212822523895 4 2.222389312692903 4.628761734497344 5 1.1915153486784629 3.102089618439888 6 0.7347075190772012 2.29535799438617 7 0.4758354457939167 1.734361103588181 8 0.3272710520788061 1.3632712450686566 9 0.23345281144231023 1.0940226480785356 >10 1.1036312836738338 10.141699578711668 >50 0.0703663271550957 2.503098114918415 >100 0.03753180486022733 3.5452855642163037 >500 0.0030533872052351595 1.1321328634478631 >1k 0.0038167340065439494 4.154642753890942 >5k 6.361223344239916E-4 2.561707304243936 >10k+ 1.272244668847983E-4 0.7059765901558734 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10657 0.7052100310948853 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 9169 0.6067439969136721 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8123 0.5375266099825236 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 7867 0.5205862170051105 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 7246 0.47949252935287034 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6265 0.4145764140761431 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 4975 0.3292127150883977 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3811 0.2521868657692229 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 3653 0.24173146697847578 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 3626 0.23994478490663926 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 3254 0.21532827636133595 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 3098 0.20500522439072488 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 2956 0.1956086001610661 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2783 0.1841606002192987 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 2426 0.16053669282501568 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1992 0.13181743285549513 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 1978 0.13089100511454285 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1976 0.13075865829440683 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1928 0.12758233461114188 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1800 0.11911213812243536 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1753 0.11600198784923844 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 1698 0.11236245029549737 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1661 0.10991403412298063 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1607 0.10634066997930758 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 1598 0.1057451092886954 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 1531 0.10131149081413808 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 6.617341006801965E-5 0.0 12-13 0.0 0.0 0.0 2.3160693523806877E-4 0.0 14-15 0.0 0.0 0.0 3.308670503400983E-4 0.0 16-17 0.0 0.0 0.0 5.293872805441572E-4 0.0 18-19 0.0 0.0 0.0 8.271676258502456E-4 0.0 20-21 0.0 0.0 0.0 0.0012572947912923732 0.0 22-23 0.0 0.0 0.0 0.0028785433379588544 0.0 24-25 0.0 0.0 0.0 0.0044336184745573165 0.0 26-27 0.0 0.0 0.0 0.005757086675917709 3.3086705034009826E-5 28-29 0.0 0.0 0.0 0.008569456603808544 6.617341006801965E-5 30-31 0.0 0.0 0.0 0.018396207998909463 6.617341006801965E-5 32-33 0.0 0.0 0.0 0.03738797668843111 6.617341006801965E-5 34-35 0.0 0.0 0.0 0.06438672799618311 6.617341006801965E-5 36-37 0.0 0.0 0.0 0.10491794166284515 6.617341006801965E-5 38-39 0.0 0.0 0.0 0.16235646160188622 6.617341006801965E-5 40-41 0.0 0.0 0.0 0.2274049236987495 6.617341006801965E-5 42-43 0.0 0.0 0.0 0.2960267499392859 6.617341006801965E-5 44-45 0.0 0.0 0.0 0.3712328304815902 6.617341006801965E-5 46-47 0.0 0.0 0.0 0.4658938935838923 6.617341006801965E-5 48-49 0.0 0.0 0.0 0.5565514653770792 6.617341006801965E-5 50-51 0.0 0.0 0.0 0.639532921602376 6.617341006801965E-5 52-53 0.0 0.0 0.0 0.7199997882450878 6.617341006801965E-5 54-55 0.0 0.0 0.0 0.8075141230600438 6.617341006801965E-5 56-57 0.0 0.0 0.0 0.8770292903364985 6.617341006801965E-5 58-59 0.0 0.0 0.0 0.9309937062469684 6.617341006801965E-5 60-61 3.3086705034009826E-5 0.0 0.0 0.9773150932945822 6.617341006801965E-5 62-63 6.617341006801965E-5 0.0 0.0 1.0195999023280469 9.926011510202948E-5 64-65 6.617341006801965E-5 3.3086705034009826E-5 0.0 1.057351832771852 1.323468201360393E-4 66-67 6.617341006801965E-5 6.617341006801965E-5 0.0 1.091133358611576 1.323468201360393E-4 68-69 6.617341006801965E-5 6.617341006801965E-5 0.0 1.1392414277310263 1.323468201360393E-4 70-71 6.617341006801965E-5 1.323468201360393E-4 0.0 1.2111388377699295 1.323468201360393E-4 72-73 6.617341006801965E-5 1.323468201360393E-4 0.0 1.3144024441810744 1.323468201360393E-4 74-75 6.617341006801965E-5 1.323468201360393E-4 0.0 1.4470801313674535 1.323468201360393E-4 76-77 6.617341006801965E-5 1.323468201360393E-4 0.0 1.6458319685067506 1.323468201360393E-4 78-79 6.617341006801965E-5 1.323468201360393E-4 0.0 1.9507921288052192 1.323468201360393E-4 80-81 6.617341006801965E-5 1.323468201360393E-4 0.0 2.3289069939338836 1.323468201360393E-4 82-83 6.617341006801965E-5 1.323468201360393E-4 0.0 2.7022242868326165 1.323468201360393E-4 84-85 6.617341006801965E-5 1.323468201360393E-4 0.0 3.0161178574902676 1.9852023020405896E-4 86-87 6.617341006801965E-5 1.9852023020405896E-4 6.617341006801965E-5 3.2615550354325524 1.9852023020405896E-4 88-89 6.617341006801965E-5 1.9852023020405896E-4 6.617341006801965E-5 3.4711923985280384 1.9852023020405896E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 9735 0.0 24.36549 1 TACATGG 9770 0.0 23.794914 2 ATGGGCG 220 2.417437E-9 23.529211 5 ACATGGG 9750 0.0 22.73284 3 AGTACTT 8055 0.0 22.04967 12-13 GAGTACT 7875 0.0 22.046503 12-13 CATGGGA 7015 0.0 20.527267 4 GTACTTT 8700 0.0 19.982561 14-15 GCTTATA 1280 0.0 19.487066 1 ATGGGAG 2610 0.0 19.11185 5 TACCTGG 1015 0.0 19.009453 2 TACGCGG 105 1.5815453E-5 18.549093 64-65 TACTTTT 9650 0.0 18.404888 14-15 CATGGGG 2875 0.0 18.332327 4 ACTTTTT 9680 0.0 18.323275 16-17 ATGGGAT 2805 0.0 17.783218 5 TAGGACG 195 2.4441315E-4 16.892767 4 AGTACAT 7530 0.0 16.874096 2 CACGTCG 145 8.549232E-7 16.674915 90-91 GCTTACG 320 0.0 16.507568 60-61 >>END_MODULE