FastQCFastQC Report
Fri 27 May 2016
SRR939654_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939654_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1497297
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42060.2809061929597134No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA38740.25873290335851873No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA29030.19388270997671136No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28210.18840617459328377No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT26500.17698559470833108No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21950.14659750203199498No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG79550.031.6373841
TACATGG94850.025.9527442
GTCCTAC3201.0094536E-625.4732191
ACATGGG96050.024.6938723
GAGTACT67800.023.18255812-13
AGTACTT71400.022.14590512-13
ATGGGAT26450.022.1280355
CATGGGA70450.021.3911634
CATGGGG27450.020.9962734
GTATAGG3404.6366287E-520.9779451
GTACTTT75550.020.80620614-15
TACGGTG1405.0640886E-420.2287965
GTCTTAG4559.4633833E-720.1546331
GGTCGGG4351.446122E-518.7389181
GCTTATA11000.018.5259781
GTCGGGA3601.03682396E-1018.3741992
GTATTAG4451.7588287E-518.317821
GTCCAAG7801.10958354E-1018.2884651
TACTTTT87100.018.18266514-15
TTAACGC2102.0504302E-517.9968054
Produced by FastQC (version 0.11.5)