FastQCFastQC Report
Fri 27 May 2016
SRR939653_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939653_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4147123
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT108510.26165127004914013No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72050.17373490007409956No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52330.1261838628851857No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA152900.033.395261
GGTATCA97050.031.7343221
ATCAACG178000.028.1529523
TATCAAC178900.028.1177652
TCAACGC177950.028.1071874
CAACGCA180800.027.6119675
AACGCAG186500.026.8910686
TACATGG132650.025.9010052
GTACATG136600.025.811791
ACGCAGA205450.023.7871827
ACATGGG139900.023.2694663
CGCAGAG207100.023.2093098
GAGTACT96100.021.1338912-13
GCAGAGT215750.020.5635879
AGAGTAC164650.019.79925310-11
CATGGGA73550.019.549464
AGTACTT105100.019.0080912-13
GTACTTT108150.018.75824714-15
CATGGGG76300.018.5338864
GTCTAGG14550.017.2859361
Produced by FastQC (version 0.11.5)