##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939653_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4147123 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.862203749442685 31.0 30.0 33.0 25.0 34.0 2 30.018933607708284 31.0 30.0 33.0 25.0 34.0 3 30.058463662640342 31.0 30.0 34.0 25.0 34.0 4 33.61872363081587 35.0 33.0 37.0 28.0 37.0 5 33.600966019093235 35.0 33.0 37.0 28.0 37.0 6 33.68260381956359 35.0 33.0 37.0 28.0 37.0 7 33.47701406493128 35.0 33.0 37.0 27.0 37.0 8 33.47091634369176 35.0 33.0 37.0 27.0 37.0 9 34.696791486531744 37.0 34.0 39.0 27.0 39.0 10-11 34.5765065323599 37.0 33.5 39.0 27.0 39.0 12-13 34.40307244323354 37.0 33.0 39.0 27.0 39.0 14-15 35.05183014827388 38.0 33.0 40.0 25.5 41.0 16-17 34.75263429611323 38.0 33.0 40.0 25.0 41.0 18-19 34.689495102990676 38.0 33.0 40.0 25.0 41.0 20-21 34.80691807308344 38.0 33.0 40.0 25.0 41.0 22-23 34.64800730530539 38.0 33.0 40.0 25.0 41.0 24-25 34.52319885375958 38.0 33.0 40.0 24.0 41.0 26-27 34.64305399188787 38.0 33.0 40.0 24.0 41.0 28-29 34.50635247616239 38.0 33.0 40.0 24.0 41.0 30-31 34.27302168274247 38.0 33.0 40.0 23.5 41.0 32-33 34.03876494620488 38.0 33.0 40.0 23.0 41.0 34-35 33.79836816993371 38.0 32.0 40.0 20.5 41.0 36-37 33.499046326815 38.0 31.5 40.0 19.0 41.0 38-39 33.33243226689925 37.5 31.0 40.0 18.5 41.0 40-41 33.03773519618299 37.0 31.0 40.0 17.0 41.0 42-43 32.713221189725985 37.0 31.0 40.0 15.0 41.0 44-45 32.455276826850806 37.0 30.5 40.0 14.5 41.0 46-47 32.014573597166034 37.0 30.0 39.5 9.0 41.0 48-49 31.65322803302434 36.0 30.0 39.0 9.0 40.5 50-51 31.052780083928063 35.5 29.0 38.5 8.0 40.0 52-53 31.105030764701215 36.0 29.0 39.0 7.5 40.0 54-55 31.11192771470728 36.0 29.0 39.0 6.5 40.0 56-57 30.950773102220502 36.0 29.0 39.0 2.0 40.0 58-59 30.518482331003927 35.0 28.0 39.0 2.0 40.0 60-61 30.072529799574305 35.0 28.0 38.5 2.0 40.0 62-63 29.632694159300314 34.5 27.0 38.0 2.0 40.0 64-65 28.987425378991652 34.0 26.0 38.0 2.0 40.0 66-67 28.419450062127407 34.0 26.0 37.5 2.0 39.5 68-69 27.87165150394623 33.0 25.0 37.0 2.0 39.0 70-71 27.290973043239855 32.5 23.5 36.0 2.0 39.0 72-73 26.5260779581411 32.0 22.0 35.5 2.0 38.5 74-75 26.041201454598767 32.0 21.0 35.0 2.0 38.0 76-77 25.62574801856612 31.0 19.5 35.0 2.0 37.0 78-79 25.02800133972395 31.0 18.0 35.0 2.0 36.5 80-81 24.329935114053768 31.0 13.0 34.0 2.0 36.0 82-83 23.637957084947807 30.0 7.5 34.0 2.0 35.5 84-85 22.951040154825407 29.5 4.0 34.0 2.0 35.0 86-87 22.242673294233136 29.0 2.0 34.0 2.0 35.0 88-89 21.4136624353799 29.0 2.0 34.0 2.0 35.0 90-91 20.183544471673493 26.5 2.0 33.0 2.0 34.5 92-93 18.647434619132348 25.0 2.0 32.0 2.0 34.0 94-95 18.25656256156376 24.0 2.0 32.0 2.0 34.0 96-97 17.553914002550684 23.0 2.0 32.0 2.0 34.0 98-99 16.526566248457062 17.5 2.0 31.5 2.0 34.0 100-101 14.937401422624793 2.0 2.0 30.0 2.0 34.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.2298915219509006 1101 2 1.2432468446146352 1101 3 1.1976897388757237 1101 4 1.26756827217757 1101 5 0.9796285838413965 1101 6 1.0589272592937533 1101 7 0.9785330867854185 1101 8 1.2335045982844974 1101 9 1.486938917364732 1101 10-11 1.3063486881953565 1101 12-13 1.1893515118101945 1101 14-15 1.3142204498783272 1101 16-17 1.2323556882826878 1101 18-19 1.237825243198472 1101 20-21 1.3937791647471798 1101 22-23 1.64607796140249 1101 24-25 1.4351665317458284 1101 26-27 1.4468973860532088 1101 28-29 1.5613381857101416 1101 30-31 1.696291185929816 1101 32-33 1.505262913660836 1101 34-35 1.5049761563004083 1101 36-37 1.4633924777965603 1101 38-39 1.3808753682334327 1101 40-41 1.4164826692347319 1101 42-43 1.5060688002394471 1101 44-45 1.3649806667530342 1101 46-47 1.4129148528136923 1101 48-49 1.323795776970048 1101 50-51 1.2719685197587154 1101 52-53 1.2199963695975207 1101 54-55 1.3302509154149469 1101 56-57 1.2209281673622563 1101 58-59 1.1869285418690012 1101 60-61 1.1574122822483268 1101 62-63 1.2365320529314445 1101 64-65 1.1334271747012465 1101 66-67 0.9941817087376386 1101 68-69 1.0302472163614382 1101 70-71 0.8360849648323168 1101 72-73 1.1074111007603094 1101 74-75 0.8733282963947246 1101 76-77 0.4173180488827235 1101 78-79 0.6974123534391374 1101 80-81 0.8002866212962587 1101 82-83 0.6694858406020145 1101 84-85 0.47182592982302296 1101 86-87 0.075594399653788 1101 88-89 -0.5697701846929135 1101 90-91 -4.086046138308168 1101 92-93 -9.177426300581477 1101 94-95 -7.530466714019051 1101 96-97 -4.329216514891868 1101 98-99 -3.2156564936097176 1101 100-101 -2.769266615451743 1102 1 1.043519529941186 1102 2 0.9667558197878172 1102 3 1.0015388546110238 1102 4 1.0304890148303656 1102 5 0.9654785617412927 1102 6 1.0482297423228317 1102 7 1.1003411357893285 1102 8 1.057324960309252 1102 9 1.1328695823557169 1102 10-11 1.0544611210109167 1102 12-13 1.0561470192228555 1102 14-15 1.2358135409468858 1102 16-17 1.3323411575580835 1102 18-19 1.1164073273982495 1102 20-21 1.1400933118159813 1102 22-23 1.3541044557156496 1102 24-25 1.2135560435159576 1102 26-27 1.0962999139277514 1102 28-29 1.2370886647525126 1102 30-31 1.3164401761127067 1102 32-33 1.4047068249397796 1102 34-35 1.247422351049842 1102 36-37 1.160141715212724 1102 38-39 1.1482167519108302 1102 40-41 1.2430372520217645 1102 42-43 1.0239226060111832 1102 44-45 0.8671523958447338 1102 46-47 1.0229869530034748 1102 48-49 0.9209458936253334 1102 50-51 1.0324099807926785 1102 52-53 1.2177174283277132 1102 54-55 1.4293324644224477 1102 56-57 1.2810029106300398 1102 58-59 1.204350520812234 1102 60-61 1.1816458951026725 1102 62-63 1.086881348718677 1102 64-65 0.97036601387628 1102 66-67 1.0384469367757063 1102 68-69 0.9776715554616437 1102 70-71 1.0138788037554463 1102 72-73 1.0257515927401215 1102 74-75 0.7717994805160089 1102 76-77 0.6281224429936962 1102 78-79 0.6167724158604493 1102 80-81 0.38443031241797243 1102 82-83 0.22017964237314658 1102 84-85 0.0039225756453582505 1102 86-87 -0.4121476126046346 1102 88-89 -0.8948638154209156 1102 90-91 -5.260628637458783 1102 92-93 -10.156205238252735 1102 94-95 -8.746873751009485 1102 96-97 -5.596986437061286 1102 98-99 -4.172630844336986 1102 100-101 -3.4760730748862034 1103 1 0.9763124480956371 1103 2 1.0087974952061494 1103 3 1.03967422533146 1103 4 1.0590855106645805 1103 5 1.0272442972310358 1103 6 0.8964925372538701 1103 7 1.0201899070558724 1103 8 0.9484875311977206 1103 9 1.063633865069427 1103 10-11 0.9584623812503636 1103 12-13 1.033678437692366 1103 14-15 1.072951878481014 1103 16-17 1.1846791642793661 1103 18-19 1.1056653176664 1103 20-21 1.254440350253283 1103 22-23 1.1645701316940844 1103 24-25 1.0525514838996344 1103 26-27 1.0800255405468135 1103 28-29 1.2259941818007576 1103 30-31 1.3398149923277884 1103 32-33 1.335785511140159 1103 34-35 1.411818398798907 1103 36-37 1.2779375651742129 1103 38-39 1.3397908697432186 1103 40-41 1.1874935112110094 1103 42-43 1.2146766930377169 1103 44-45 1.0487115483337064 1103 46-47 1.1622571955995724 1103 48-49 0.9730425732444594 1103 50-51 0.8971744735463005 1103 52-53 1.0128311561860066 1103 54-55 1.1756156332245276 1103 56-57 1.0040585099439063 1103 58-59 0.9622528347518831 1103 60-61 0.8832638587657655 1103 62-63 1.0583441454000457 1103 64-65 0.7696932297973049 1103 66-67 0.7378316948797483 1103 68-69 0.7534990251808864 1103 70-71 0.9595574514485108 1103 72-73 0.833065794938534 1103 74-75 0.6004031964720369 1103 76-77 0.37867882995722013 1103 78-79 0.3488991136830357 1103 80-81 0.189746509995512 1103 82-83 0.18748386767595449 1103 84-85 0.06945240131223684 1103 86-87 -0.16782656660589268 1103 88-89 -0.7811910926035814 1103 90-91 -2.2758488730535475 1103 92-93 -8.683175456344172 1103 94-95 -5.3125648260637455 1103 96-97 -2.846912266718922 1103 98-99 -2.0333096233049908 1103 100-101 -1.512895039809539 1104 1 1.034163237761316 1104 2 1.0105305216856983 1104 3 1.017859174475273 1104 4 1.065052797476305 1104 5 0.95829446675166 1104 6 0.9514789112292377 1104 7 1.1503946192999805 1104 8 1.1513747463387034 1104 9 1.2618273438900829 1104 10-11 1.0620738142360722 1104 12-13 1.0446964733989859 1104 14-15 1.2076133468729253 1104 16-17 1.3169350223970397 1104 18-19 1.3421539608576794 1104 20-21 1.3357338033239685 1104 22-23 1.197842577082696 1104 24-25 1.2039815459141394 1104 26-27 1.1164318374868571 1104 28-29 1.229744557321112 1104 30-31 1.2308137692043175 1104 32-33 1.2900040800857013 1104 34-35 1.1309492837394757 1104 36-37 1.21708730660162 1104 38-39 1.0436062907551857 1104 40-41 1.1112872861562906 1104 42-43 1.0140239581765513 1104 44-45 1.118944987885662 1104 46-47 0.948785536657887 1104 48-49 1.1006571337783448 1104 50-51 1.1302688149908704 1104 52-53 1.210080603634605 1104 54-55 1.210848563141699 1104 56-57 1.1712360184199362 1104 58-59 1.0587602271384746 1104 60-61 1.138137442517536 1104 62-63 0.9933234649571467 1104 64-65 1.1706906387067946 1104 66-67 1.2202793366118634 1104 68-69 1.2671998856589823 1104 70-71 1.3487265053517774 1104 72-73 1.1490272880075025 1104 74-75 0.8988237309984548 1104 76-77 0.9827214407893727 1104 78-79 0.8151287014689679 1104 80-81 0.6626331350330652 1104 82-83 0.7658262671437868 1104 84-85 0.595059134928821 1104 86-87 0.3856255384539793 1104 88-89 0.30649988632436376 1104 90-91 -0.13458629312135528 1104 92-93 -3.5502998555115894 1104 94-95 -1.2418560850225902 1104 96-97 -0.4349896292784692 1104 98-99 -0.18565179746060778 1104 100-101 -0.1037743207416888 1105 1 0.9300737388221272 1105 2 1.0036596209714546 1105 3 0.9444030536001584 1105 4 0.9330054976251887 1105 5 0.8333961240403553 1105 6 0.8550494183363995 1105 7 0.9704269475470468 1105 8 0.9844496824373223 1105 9 1.199113030116969 1105 10-11 1.0427749638259485 1105 12-13 1.0275617459831707 1105 14-15 1.1570270157831501 1105 16-17 1.177600817863464 1105 18-19 1.1274840799178136 1105 20-21 1.0695123367303196 1105 22-23 1.0823453080712042 1105 24-25 1.1323389516249946 1105 26-27 1.119749873033875 1105 28-29 1.1538174031335302 1105 30-31 1.155550659797548 1105 32-33 1.1532742905759221 1105 34-35 1.1181855157993326 1105 36-37 1.1981924106877173 1105 38-39 1.183711121161572 1105 40-41 0.9678280556528662 1105 42-43 1.2655364235714686 1105 44-45 1.1290228582738635 1105 46-47 0.9697863884961251 1105 48-49 0.9679533538373626 1105 50-51 0.8946967203792795 1105 52-53 0.8695610390474151 1105 54-55 1.105689443920273 1105 56-57 0.9647253050614815 1105 58-59 0.8918779847911722 1105 60-61 0.8174520112747672 1105 62-63 0.929853060324529 1105 64-65 1.0758680212534841 1105 66-67 1.0604760780131706 1105 68-69 0.9663549637144335 1105 70-71 1.0004620210624253 1105 72-73 1.0938999810131627 1105 74-75 0.99754578453107 1105 76-77 0.7989987104870835 1105 78-79 0.7161502589469784 1105 80-81 0.7450358267662374 1105 82-83 0.7685503663849715 1105 84-85 0.7865325935352381 1105 86-87 0.3322909821622737 1105 88-89 0.23233111752914581 1105 90-91 -0.8307339742599069 1105 92-93 -6.608747629560755 1105 94-95 -2.5648681720478734 1105 96-97 -0.9480392614858992 1105 98-99 -0.3860049334437612 1105 100-101 -0.3298671551692447 1106 1 0.7741287061019833 1106 2 0.7551178690893572 1106 3 0.8028320045143751 1106 4 0.8739708018204198 1106 5 0.79253510178836 1106 6 0.8338303611669389 1106 7 0.8079336102911938 1106 8 0.8298844117963071 1106 9 0.946025537883564 1106 10-11 0.9606702008309327 1106 12-13 1.0023504233833975 1106 14-15 1.0086977640678327 1106 16-17 1.0843263434693 1106 18-19 0.9924442707817036 1106 20-21 0.9421675870537882 1106 22-23 0.9581639567078426 1106 24-25 0.883425353936282 1106 26-27 0.8883277340222904 1106 28-29 0.859543408390607 1106 30-31 0.9053729190499595 1106 32-33 1.0667855506596027 1106 34-35 0.8242801234859769 1106 36-37 0.9756500114570414 1106 38-39 1.07031996922683 1106 40-41 1.057227984460738 1106 42-43 1.1550656009544866 1106 44-45 1.055969623473345 1106 46-47 1.0381565922693952 1106 48-49 1.1474624790418098 1106 50-51 0.895878894454718 1106 52-53 0.9854198480631418 1106 54-55 1.1450318285852354 1106 56-57 1.1908551004405048 1106 58-59 1.0913589781225674 1106 60-61 0.9640983557271596 1106 62-63 0.9947133404328916 1106 64-65 0.8487017089134739 1106 66-67 0.7528263963469435 1106 68-69 0.7892201379313235 1106 70-71 0.6958197976154139 1106 72-73 0.863671920745027 1106 74-75 0.8780250554081164 1106 76-77 0.7009078948979095 1106 78-79 0.7231474026374975 1106 80-81 0.7314914105236099 1106 82-83 0.8582966362618052 1106 84-85 0.5947742652248387 1106 86-87 0.24584584355324068 1106 88-89 0.3892651959644695 1106 90-91 -0.08482304343797153 1106 92-93 -3.2134025980052776 1106 94-95 -1.1638321194795118 1106 96-97 -0.3790823013203912 1106 98-99 -0.14222198967072686 1106 100-101 -0.10095830165659159 1107 1 0.7013974223103574 1107 2 0.6575446487792505 1107 3 0.6784500943288485 1107 4 0.6779878782220052 1107 5 0.59744890509387 1107 6 0.5097527006722586 1107 7 0.5699337286351351 1107 8 0.5248588392145948 1107 9 0.5547140840768634 1107 10-11 0.48081018112981155 1107 12-13 0.6068270044327022 1107 14-15 0.6936263560779636 1107 16-17 0.7322994676749843 1107 18-19 0.6353830609521793 1107 20-21 0.629270820416032 1107 22-23 0.6964262531862815 1107 24-25 0.681586084730121 1107 26-27 0.5823677731501178 1107 28-29 0.5778242668586842 1107 30-31 0.6391770578290945 1107 32-33 0.5448666594222615 1107 34-35 0.5653592701404122 1107 36-37 0.6792333173006639 1107 38-39 0.4631087570247061 1107 40-41 0.4259114838853151 1107 42-43 0.4842707841903504 1107 44-45 0.3791609907689093 1107 46-47 0.426725625376541 1107 48-49 0.3697283695742364 1107 50-51 0.4700082589591972 1107 52-53 0.43766560297456536 1107 54-55 0.35922188866622307 1107 56-57 0.30147809838406303 1107 58-59 0.36936597184919506 1107 60-61 0.28486560178364684 1107 62-63 0.23389480342697055 1107 64-65 0.3041599014870755 1107 66-67 0.3102230283470284 1107 68-69 0.37018536568051985 1107 70-71 0.29653735065150855 1107 72-73 0.4628406760583559 1107 74-75 0.2760045041540451 1107 76-77 0.12896535631178452 1107 78-79 0.07201440234421241 1107 80-81 0.0637247264579095 1107 82-83 0.05466582132942932 1107 84-85 -0.11132417092003166 1107 86-87 -0.09751312477346374 1107 88-89 -0.2099751660079079 1107 90-91 -0.5915584286760804 1107 92-93 -4.102920240685474 1107 94-95 -1.455659519989137 1107 96-97 -0.7153075098372668 1107 98-99 -0.45398891471213076 1107 100-101 -0.4639806586562347 1108 1 0.3687254011446015 1108 2 0.2975364865396628 1108 3 0.06258330159487357 1108 4 -0.40515992063556183 1108 5 -0.9650591371424397 1108 6 -0.2883118885192175 1108 7 -0.05774153766120804 1108 8 0.12177736687323204 1108 9 0.07298508696944594 1108 10-11 0.07556774224374863 1108 12-13 0.04842390616998671 1108 14-15 0.06186686906271888 1108 16-17 0.10080691231515004 1108 18-19 0.12997472005897492 1108 20-21 0.12886177563806456 1108 22-23 -0.050910934638814354 1108 24-25 0.024999533527449103 1108 26-27 -0.04465377782464941 1108 28-29 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84-85 0.5676395826237517 2311 86-87 0.6769662117647925 2311 88-89 0.7681749890597267 2311 90-91 1.7259640691576053 2311 92-93 2.8211682183069975 2311 94-95 2.158321004403863 2311 96-97 1.7454951653930202 2311 98-99 1.6164285086369823 2311 100-101 1.6674718966306354 2312 1 0.22417030999413257 2312 2 -0.009732236085962853 2312 3 0.008797236932942099 2312 4 -0.06829334154608091 2312 5 0.07927954022429873 2312 6 0.18318721093724122 2312 7 0.15449202402752604 2312 8 -0.5130392000545214 2312 9 0.05581557180307328 2312 10-11 0.0016450149066287167 2312 12-13 0.11822449478282238 2312 14-15 -0.1609932985313023 2312 16-17 0.06800277987557735 2312 18-19 0.1633289992435678 2312 20-21 0.2352194137624295 2312 22-23 0.11643469135601237 2312 24-25 0.2869224971356914 2312 26-27 0.15525565157818022 2312 28-29 0.015900193840778343 2312 30-31 0.3219717319522388 2312 32-33 0.2277224056503897 2312 34-35 0.19443735386808214 2312 36-37 0.2546816498132145 2312 38-39 0.3683957764529353 2312 40-41 0.4730566914526051 2312 42-43 0.5706671796222551 2312 44-45 0.37029950132976097 2312 46-47 0.545026526421232 2312 48-49 0.43855519633580187 2312 50-51 0.42642411473754294 2312 52-53 0.4164826581993175 2312 54-55 0.40479625356499227 2312 56-57 0.4843358641280169 2312 58-59 0.43665307595109937 2312 60-61 0.46374428873825835 2312 62-63 0.4098350705286187 2312 64-65 0.45381631615061124 2312 66-67 0.5368624128959318 2312 68-69 0.2121106844720444 2312 70-71 0.20429579514743068 2312 72-73 0.4671239717913842 2312 74-75 0.6429221585180471 2312 76-77 0.7737592878370556 2312 78-79 0.844218029881258 2312 80-81 0.7908495977441881 2312 82-83 0.9163139019621269 2312 84-85 0.9728865001042486 2312 86-87 0.812048747351767 2312 88-89 1.1003746092662112 2312 90-91 1.5844047100505492 2312 92-93 2.5991099810038314 2312 94-95 2.017675401381137 2312 96-97 1.513582515603769 2312 98-99 1.639601312388507 2312 100-101 1.5314249428480355 2313 1 -0.1854965734927987 2313 2 -0.9216706668061327 2313 3 -0.29158394460317894 2313 4 -0.08148514689137443 2313 5 -0.6705493252684676 2313 6 -0.2656252088344644 2313 7 -1.140487454797963 2313 8 -1.0077780659502267 2313 9 -1.2658945068854166 2313 10-11 -2.132963643705075 2313 12-13 -0.5639772862133654 2313 14-15 -0.12160657368773542 2313 16-17 -1.6068857495649596 2313 18-19 -1.2893813499905562 2313 20-21 -1.5298310401210458 2313 22-23 -1.1849513081950036 2313 24-25 -0.35640486884348377 2313 26-27 -0.8931541732579049 2313 28-29 -2.3032002131289318 2313 30-31 -0.4712738371433929 2313 32-33 -0.13227264394005545 2313 34-35 0.1496721817262241 2313 36-37 0.08679092722610449 2313 38-39 -0.21462869353664615 2313 40-41 -0.1775503093475166 2313 42-43 0.1513558621801181 2313 44-45 -0.38563448553438207 2313 46-47 -0.5694551484289541 2313 48-49 0.018682180097300716 2313 50-51 0.01055459832348049 2313 52-53 0.12955266044427205 2313 54-55 0.2777254569519947 2313 56-57 -0.17890135834684529 2313 58-59 0.05605234950727933 2313 60-61 0.09966054622863041 2313 62-63 0.1542929719003432 2313 64-65 0.19695073128274743 2313 66-67 0.03236179985102794 2313 68-69 0.06805215451343116 2313 70-71 0.02185109870539037 2313 72-73 0.04813684861252909 2313 74-75 0.18262628520248114 2313 76-77 0.29771316844008666 2313 78-79 0.2578985442633517 2313 80-81 0.09265327371110033 2313 82-83 -0.05708708696852938 2313 84-85 0.20570760036155278 2313 86-87 0.2461083710055192 2313 88-89 0.4127240391056688 2313 90-91 1.14844838878048 2313 92-93 2.3068922550838344 2313 94-95 1.4419413880380567 2313 96-97 0.8536911943624794 2313 98-99 0.7568102024030701 2313 100-101 0.7442666506242102 2314 1 -0.7126751413102141 2314 2 -0.7035453806525034 2314 3 -1.9743872788113066 2314 4 -0.6777235857166986 2314 5 -0.4357824005948956 2314 6 -3.1041032066736136 2314 7 -3.9696762497318936 2314 8 -1.606276649809292 2314 9 -2.008483460190824 2314 10-11 -1.1610723542499528 2314 12-13 -2.253726402616401 2314 14-15 -3.066387801782426 2314 16-17 -2.351807716961339 2314 18-19 -2.83852706424706 2314 20-21 -2.2920794606666206 2314 22-23 -3.4474469881982728 2314 24-25 -2.1147022959318207 2314 26-27 -2.237652695767899 2314 28-29 -1.527405896097413 2314 30-31 -1.571967990463797 2314 32-33 -2.441540147453427 2314 34-35 -0.9132192224743676 2314 36-37 -0.5520963824923868 2314 38-39 -0.6392357130058812 2314 40-41 -0.8640825988378538 2314 42-43 -0.8367783650318223 2314 44-45 -1.366868306138354 2314 46-47 -1.696468505483928 2314 48-49 -2.0221855001922684 2314 50-51 -1.1593118765705483 2314 52-53 -0.8106376579914638 2314 54-55 -0.4462516212192149 2314 56-57 -0.5943505527995256 2314 58-59 -0.608755326851405 2314 60-61 -0.7346741856361909 2314 62-63 -0.6636928473766694 2314 64-65 -0.7522136376341813 2314 66-67 -0.6424839697332416 2314 68-69 -0.585172545529403 2314 70-71 -0.693739721202153 2314 72-73 -0.5612905714661096 2314 74-75 -0.30265364263284056 2314 76-77 -0.3674132049553229 2314 78-79 -0.31851621632192106 2314 80-81 -0.35744871343058904 2314 82-83 -0.37146512672953946 2314 84-85 -0.44640961270943436 2314 86-87 -0.24515554472358758 2314 88-89 -0.0901183994515371 2314 90-91 0.5978886012754678 2314 92-93 1.527101569008142 2314 94-95 0.9177780586469702 2314 96-97 0.6952516646598745 2314 98-99 0.6494164584172673 2314 100-101 0.8378279525422752 2315 1 -7.236270476509784 2315 2 -3.1842152225200664 2315 3 -1.5375871404485828 2315 4 -1.0934843660882336 2315 5 -1.679026532172223 2315 6 -1.9205852153714211 2315 7 -3.374575231597216 2315 8 -3.011769967387451 2315 9 -3.386209324894729 2315 10-11 -2.427835015467238 2315 12-13 -2.841079076613113 2315 14-15 -4.9358418632658285 2315 16-17 -4.572627240172629 2315 18-19 -3.5117502249985044 2315 20-21 -4.439479429033643 2315 22-23 -5.9111588974199165 2315 24-25 -3.9001616441265234 2315 26-27 -4.870293778135199 2315 28-29 -3.2423710637504044 2315 30-31 -2.9456099274986585 2315 32-33 -2.0875644464150227 2315 34-35 -2.7795020281645506 2315 36-37 -3.1533869097676224 2315 38-39 -2.52984870297891 2315 40-41 -3.7937116859216076 2315 42-43 -7.883664225342464 2315 44-45 -4.461515885169405 2315 46-47 -3.10427329393071 2315 48-49 -5.709405510450441 2315 50-51 -6.849150512305428 2315 52-53 -7.081650820485063 2315 54-55 -4.744515788519802 2315 56-57 -4.038616024735084 2315 58-59 -4.395379766015271 2315 60-61 -3.3233180361597228 2315 62-63 -3.6248188191349477 2315 64-65 -2.3118898441510076 2315 66-67 -2.953256464587021 2315 68-69 -2.380254433286069 2315 70-71 -1.9474532147779904 2315 72-73 -2.5194616369925456 2315 74-75 -3.035181087939705 2315 76-77 -3.0082691908564634 2315 78-79 -2.1110551795320767 2315 80-81 -1.7216825442709762 2315 82-83 -2.2867352262057032 2315 84-85 -1.9417223770314713 2315 86-87 -2.351428717143527 2315 88-89 -1.8720245831399573 2315 90-91 -0.8960453375570978 2315 92-93 -0.0634989409068325 2315 94-95 -0.2919545245483093 2315 96-97 -0.38309235066534697 2315 98-99 -0.44683930323959586 2315 100-101 -0.4023268687097463 2316 1 -4.438438684040868 2316 2 -3.1099484614491963 2316 3 -4.900312431631178 2316 4 -4.609023619207655 2316 5 -4.131934095135129 2316 6 -4.5757438366746435 2316 7 -3.1556470652876527 2316 8 -1.777458611451479 2316 9 -2.0672946890305255 2316 10-11 -3.2306933683857366 2316 12-13 -4.041480263585335 2316 14-15 -3.115987046797976 2316 16-17 -3.080510670752176 2316 18-19 -3.2780293895954458 2316 20-21 -5.69646030476855 2316 22-23 -6.723895216842223 2316 24-25 -4.426024349029362 2316 26-27 -4.010224439236467 2316 28-29 -4.499662861705637 2316 30-31 -6.213090150806611 2316 32-33 -4.585734685366393 2316 34-35 -6.237873296171166 2316 36-37 -9.506105880564103 2316 38-39 -6.383157638262606 2316 40-41 -5.70854300019122 2316 42-43 -4.921248929637358 2316 44-45 -3.6140568286677492 2316 46-47 -5.9709806137163 2316 48-49 -3.3120349578892956 2316 50-51 -4.443154856875829 2316 52-53 -5.51606207929839 2316 54-55 -7.226254413678273 2316 56-57 -5.066965366006283 2316 58-59 -6.070024174790412 2316 60-61 -6.467398744339523 2316 62-63 -6.563415619277297 2316 64-65 -5.2547169473011905 2316 66-67 -6.662408884127206 2316 68-69 -5.3980728250536245 2316 70-71 -6.205781481917171 2316 72-73 -8.100108837117098 2316 74-75 -7.944487532138666 2316 76-77 -7.243391261422371 2316 78-79 -6.479287547322947 2316 80-81 -6.6860833864948646 2316 82-83 -5.523644427649938 2316 84-85 -6.63450805477704 2316 86-87 -5.394591486547668 2316 88-89 -5.3357984838376105 2316 90-91 -5.867114743181318 2316 92-93 -4.535605001063564 2316 94-95 -4.532461619691436 2316 96-97 -5.057873268659726 2316 98-99 -5.809939091200835 2316 100-101 -4.98014546436073 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 28516.0 3 11898.0 4 12185.0 5 13749.0 6 15650.0 7 17893.0 8 21672.0 9 26304.0 10 30798.0 11 35665.0 12 40247.0 13 44313.0 14 47555.0 15 52106.0 16 57224.0 17 62043.0 18 60400.0 19 60214.0 20 64402.0 21 69630.0 22 76290.0 23 83522.0 24 92819.0 25 103518.0 26 115470.0 27 129461.0 28 145071.0 29 163772.0 30 185278.0 31 209287.0 32 239120.0 33 273567.0 34 318995.0 35 375076.0 36 417349.0 37 344521.0 38 99986.0 39 1557.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.79457259663063 18.916821793602857 13.513891191758615 23.774714418007903 2 15.287882265848024 20.10513736431504 40.79560488259484 23.811375487242096 3 19.580505830799996 24.77494427461662 29.23022337219729 26.414326522386094 4 12.784186565851051 16.38755793192822 37.57819497857716 33.25006052364357 5 13.912007503513049 37.34074223183171 34.44266478982295 14.304585474832287 6 31.820082798497513 36.240064964603704 17.913337894369775 14.026514342529008 7 28.12858486510831 32.479019676889386 21.4384376462456 17.953957811756705 8 25.71952707620958 35.45089365875886 20.125616158261685 18.703963106769873 9 26.523892863900162 16.517603743932906 20.24271272960356 36.71579066256337 10-11 24.948152170633843 26.421033577699337 27.837547892720305 20.79326635894651 12-13 25.948189757297648 23.71517314790468 27.991024780684793 22.345612314112888 14-15 23.003012055826154 25.02191853196944 26.079836917674537 25.895232494529868 16-17 22.58657154779986 28.000049236032957 26.638057474379767 22.77532174178742 18-19 22.482357762705092 27.044503475830894 28.51700697676327 21.956131784700744 20-21 23.411736137495613 26.27755567860724 28.353014897675337 21.95769328622181 22-23 22.82663838669966 26.277133939931545 28.26479058408424 22.631437089284557 24-25 22.712340615206532 26.372827432571665 28.517838227045416 22.396993725176394 26-27 22.653019927761083 26.53097198967077 28.407636037165517 22.40837204540263 28-29 22.513845657158456 26.790578421336107 28.37964006543387 22.31593585607157 30-31 22.76605696549003 26.591461575593023 28.256277333503334 22.386204125413617 32-33 22.59921936394666 26.596824985183034 28.219357064872185 22.584598585998126 34-35 22.810867663248615 26.672537198580397 28.182887270696433 22.333707867474555 36-37 22.950522394672923 26.449126372208102 28.224440039609703 22.375911193509264 38-39 22.70131503813043 26.708638109184502 28.233770874555287 22.35627597812978 40-41 22.829441479135973 26.631038701651605 28.236989143208607 22.30253067600382 42-43 22.64771528454054 26.659244883007315 28.082228244474134 22.610811587978013 44-45 22.675996001713553 26.82374140101048 27.962789525416532 22.537473071859434 46-47 22.874838447965608 26.823161113783943 27.64026278124844 22.661737657002018 48-49 22.8086152648308 26.855317729217525 27.685091637019376 22.650975368932297 50-51 22.705592522371298 27.060550436557296 27.641624500344175 22.592232540727235 52-53 22.841127527257495 27.09722939485918 27.448706050925104 22.612937026958218 54-55 22.799493012619735 27.039661074467848 27.40328382416789 22.75756208874453 56-57 22.698218433956082 27.289412514100054 27.342402039704357 22.669967012239507 58-59 22.79742918214837 27.01720083723731 27.473798909968984 22.711571070645338 60-61 22.722868696652984 27.045653566374778 27.620515096211445 22.6109626407608 62-63 22.671408029187916 26.94458554684369 27.64823316726171 22.735773256706683 64-65 22.62692968890218 27.019271632775016 27.582887260338733 22.770911417984067 66-67 22.63443070094273 27.1740233935283 27.2859149327341 22.905630972794867 68-69 22.652232348391735 27.02280959316748 27.45850930198478 22.866448756456005 70-71 22.690746859073847 26.851924731733178 27.34766665089242 23.109661758300554 72-73 22.685709628046403 26.83858463823505 27.33702237880037 23.138683354918175 74-75 22.577562773153858 27.147296685608396 27.106294923800732 23.168845617437018 76-77 22.57668756114352 26.992270251337608 27.19181854415015 23.23922364336872 78-79 22.575480345943284 27.074904860766402 27.191825574247247 23.157789219043064 80-81 22.523724901656294 27.010545836699627 27.1118336020531 23.353895659590975 82-83 22.60115104264172 26.999969804757562 27.20373937882347 23.195139773777242 84-85 22.61016000330377 26.981952686086284 27.04048105562139 23.367406254988556 86-87 22.434966007243045 26.80946027585206 27.305226471664007 23.450347245240888 88-89 22.603512790247002 26.741959090195948 27.090211409231017 23.56431671032603 90-91 22.23727671072643 26.82777548293538 27.370063464581797 23.564884341756393 92-93 22.010679240937495 26.76652948912984 27.714072718236533 23.508718551696134 94-95 22.34924579568733 26.748705531499933 27.16737041999224 23.734678252820498 96-97 22.360617654189173 26.61161960342874 27.14662468217804 23.881138060204048 98-99 22.353796564746187 26.619948935253028 26.9402483501457 24.086006149855084 100-101 22.348908839765656 26.599513697378836 27.0196396276223 24.03193783523321 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3585.0 1 2093.0 2 655.5 3 722.0 4 677.5 5 668.0 6 892.0 7 1554.0 8 2493.0 9 2870.0 10 2607.0 11 2415.5 12 2527.0 13 2678.5 14 2883.0 15 3179.5 16 3590.0 17 3942.5 18 4300.0 19 4944.5 20 5763.5 21 6839.5 22 8318.5 23 10318.0 24 12756.0 25 15999.0 26 21154.0 27 27478.5 28 33162.5 29 39318.5 30 45912.0 31 53380.0 32 61619.5 33 68975.5 34 77067.5 35 84826.5 36 92358.0 37 100051.0 38 106967.0 39 112580.0 40 118320.5 41 124323.5 42 128652.5 43 133601.0 44 139691.5 45 144880.5 46 150034.0 47 155539.0 48 162103.0 49 164175.0 50 160155.0 51 157791.0 52 156071.0 53 153070.0 54 148271.5 55 139621.5 56 128377.0 57 116693.5 58 105569.0 59 93715.0 60 79390.0 61 64703.5 62 51353.0 63 39317.5 64 28999.0 65 20854.0 66 14771.5 67 10635.5 68 7620.5 69 5433.0 70 3817.0 71 2500.0 72 1609.0 73 1022.0 74 690.0 75 462.5 76 304.5 77 193.0 78 120.0 79 85.5 80 57.5 81 36.5 82 28.0 83 22.5 84 18.5 85 17.5 86 13.0 87 12.0 88 9.5 89 6.0 90 5.0 91 6.0 92 5.5 93 3.5 94 4.0 95 2.5 96 1.5 97 1.0 98 2.0 99 2.5 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.09023122776922701 2 0.10035873061879283 3 0.09736870596796864 4 0.0992013017216996 5 0.09638006878503483 6 0.08024840353179782 7 0.09249785935936794 8 0.08938726919842985 9 0.08340721989678146 10-11 0.09037590638136367 12-13 0.08956811746360066 14-15 0.09499356541872522 16-17 0.09201559731891241 18-19 0.09502973507175938 20-21 0.1043856186565964 22-23 0.11411525532278642 24-25 0.13628725263272876 26-27 0.14142334336358 28-29 0.14088079856806754 30-31 0.14260488536269603 32-33 0.1388070717941088 34-35 0.13342985004302985 36-37 0.13778226495814086 38-39 0.13064478675939922 40-41 0.1264852766604704 42-43 0.1268710862928348 44-45 0.11691237515742842 46-47 0.13637164848980848 48-49 0.1532749330077743 50-51 0.16331804000026043 52-53 0.17267392358509742 54-55 0.16832150866998638 56-57 0.13837303595769887 58-59 0.1303554295351259 60-61 0.12673846423170954 62-63 0.12382077888695368 64-65 0.11052240312139283 66-67 0.12455622849864834 68-69 0.12177116521501774 70-71 0.12902920892387323 72-73 0.15638552316871238 74-75 0.17016616097472875 76-77 0.17537459101164832 78-79 0.1721193222385736 80-81 0.17922063078428105 82-83 0.1783887287644953 84-85 0.15422740053767395 86-87 0.15580680872016578 88-89 0.17623060613345687 90-91 0.266124250474365 92-93 0.5376739489038546 94-95 0.17594124890918356 96-97 0.18848006196102696 98-99 0.20861450215004473 100-101 0.2160292810220483 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 4147123.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.34164635914463 #Duplication Level Percentage of deduplicated Percentage of total 1 75.14583645751104 41.58694306593694 2 13.083942796854439 14.481738704935927 3 4.816716751268521 7.996951051826115 4 2.2993086396360987 5.089901024210676 5 1.2864681153796524 3.559763174682801 6 0.7689331183011947 2.553241482411543 7 0.5373450502538424 2.081629182078947 8 0.38315906746883477 1.6963722888927917 9 0.2809273402837581 1.3992283366738947 >10 1.2929548768795616 12.513116858063903 >50 0.07082127732584255 2.6948469638995465 >100 0.031866041726817486 3.1126186230771227 >500 0.0013234362985906916 0.48090133029266724 >1k 2.6468720790650397E-4 0.18417702642630074 >5k 8.822906930214239E-5 0.3036577219896329 >10k+ 4.4114534651071196E-5 0.2649131646011824 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10851 0.26165127004914013 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7205 0.17373490007409956 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5233 0.1261838628851857 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 4.822620404555158E-5 2 0.0 0.0 0.0 0.0 7.233930606832737E-5 3 0.0 0.0 0.0 0.0 7.233930606832737E-5 4 0.0 0.0 0.0 0.0 7.233930606832737E-5 5 0.0 0.0 0.0 0.0 7.233930606832737E-5 6 0.0 0.0 0.0 0.0 7.233930606832737E-5 7 2.411310202277579E-5 0.0 2.411310202277579E-5 0.0 7.233930606832737E-5 8 2.411310202277579E-5 0.0 2.411310202277579E-5 0.0 7.233930606832737E-5 9 2.411310202277579E-5 0.0 2.411310202277579E-5 7.233930606832737E-5 7.233930606832737E-5 10-11 2.411310202277579E-5 0.0 2.411310202277579E-5 1.0850895910249106E-4 9.645240809110316E-5 12-13 3.616965303416369E-5 0.0 2.411310202277579E-5 1.6879171415943054E-4 3.8580963236441266E-4 14-15 7.233930606832737E-5 0.0 2.411310202277579E-5 2.170179182049821E-4 4.340358364099642E-4 16-17 7.233930606832737E-5 0.0 2.411310202277579E-5 3.014137752846974E-4 5.666578975352311E-4 18-19 9.645240809110316E-5 0.0 2.411310202277579E-5 3.616965303416368E-4 6.028275505693948E-4 20-21 9.645240809110316E-5 0.0 2.411310202277579E-5 5.425447955124552E-4 6.751668566377222E-4 22-23 9.645240809110316E-5 0.0 2.411310202277579E-5 8.319020197857646E-4 7.836758157402132E-4 24-25 1.2056551011387895E-4 0.0 2.411310202277579E-5 0.0011574288970932379 7.95732366751601E-4 26-27 1.2056551011387895E-4 0.0 2.411310202277579E-5 0.001519125427434875 8.680716728199285E-4 28-29 1.4467861213665473E-4 2.411310202277579E-5 2.411310202277579E-5 0.0027609501816078276 9.1629787686548E-4 30-31 1.4467861213665473E-4 2.411310202277579E-5 2.411310202277579E-5 0.006763725117388609 9.404109788882559E-4 32-33 1.4467861213665473E-4 2.411310202277579E-5 2.411310202277579E-5 0.015950816988066184 9.645240809110316E-4 34-35 1.4467861213665473E-4 2.411310202277579E-5 2.411310202277579E-5 0.029502380324866177 0.0010006937339451952 36-37 1.6879171415943052E-4 2.411310202277579E-5 2.411310202277579E-5 0.05187933900200211 0.001012750284956583 38-39 1.6879171415943052E-4 2.411310202277579E-5 2.411310202277579E-5 0.08360012471296366 0.0010730330400135226 40-41 1.6879171415943052E-4 2.411310202277579E-5 2.411310202277579E-5 0.11905844123745546 0.0010850895910249105 42-43 1.808482651708184E-4 2.411310202277579E-5 2.411310202277579E-5 0.15605999629140493 0.0010971461420362983 44-45 2.411310202277579E-4 2.411310202277579E-5 2.411310202277579E-5 0.20263445284839635 0.001157428897093238 46-47 2.652441222505337E-4 2.411310202277579E-5 2.411310202277579E-5 0.259348468805965 0.0012297682031615653 48-49 2.652441222505337E-4 4.822620404555158E-5 2.411310202277579E-5 0.3221148733712504 0.0013021075092298927 50-51 2.652441222505337E-4 4.822620404555158E-5 2.411310202277579E-5 0.3882329991177016 0.0013262206112526684 52-53 2.652441222505337E-4 4.822620404555158E-5 2.411310202277579E-5 0.45722058400486315 0.0013503337132754441 54-55 2.652441222505337E-4 7.233930606832737E-5 2.411310202277579E-5 0.5330321767644702 0.0013985599173209958 56-57 2.652441222505337E-4 9.645240809110316E-5 2.411310202277579E-5 0.6221542018406495 0.0014347295703551594 58-59 2.652441222505337E-4 9.645240809110316E-5 2.411310202277579E-5 0.7231880993160801 0.0014467861213665473 60-61 2.652441222505337E-4 9.645240809110316E-5 2.411310202277579E-5 0.8335778803763476 0.0014588426723779351 62-63 2.652441222505337E-4 9.645240809110316E-5 2.411310202277579E-5 0.9496824666160131 0.0015432385294576506 64-65 2.652441222505337E-4 9.645240809110316E-5 2.411310202277579E-5 1.0715380276881106 0.001591464733503202 66-67 2.652441222505337E-4 9.645240809110316E-5 2.411310202277579E-5 1.2081508071981468 0.001591464733503202 68-69 2.8935722427330947E-4 1.0850895910249106E-4 2.411310202277579E-5 1.366742679201943 0.001591464733503202 70-71 2.8935722427330947E-4 1.2056551011387895E-4 2.411310202277579E-5 1.5432746991106847 0.00160352128451459 72-73 3.014137752846974E-4 1.2056551011387895E-4 2.411310202277579E-5 1.727317950299521 0.0016758605905829174 74-75 3.1347032629608525E-4 1.2056551011387895E-4 2.411310202277579E-5 1.9188000934623834 0.001724086794628469 76-77 3.1347032629608525E-4 1.2056551011387895E-4 2.411310202277579E-5 2.1286323072645787 0.0017602564476626326 78-79 3.1347032629608525E-4 1.2056551011387895E-4 2.411310202277579E-5 2.366483945617239 0.0017723129986740205 80-81 3.1347032629608525E-4 1.2056551011387895E-4 2.411310202277579E-5 2.6250487386074637 0.0018325957537309598 82-83 3.1347032629608525E-4 1.2056551011387895E-4 3.616965303416369E-5 2.8953686688337914 0.002025500569913166 84-85 3.255268773074731E-4 1.2056551011387895E-4 4.822620404555158E-5 3.1646637922241516 0.002025500569913166 86-87 3.3758342831886103E-4 1.2056551011387895E-4 4.822620404555158E-5 3.4501870332758395 0.0020978398759814937 88-89 3.3758342831886103E-4 1.2056551011387895E-4 4.822620404555158E-5 3.7618850465732505 0.002170179182049821 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 15290 0.0 33.39526 1 GGTATCA 9705 0.0 31.734322 1 ATCAACG 17800 0.0 28.152952 3 TATCAAC 17890 0.0 28.117765 2 TCAACGC 17795 0.0 28.107187 4 CAACGCA 18080 0.0 27.611967 5 AACGCAG 18650 0.0 26.891068 6 TACATGG 13265 0.0 25.901005 2 GTACATG 13660 0.0 25.81179 1 ACGCAGA 20545 0.0 23.787182 7 ACATGGG 13990 0.0 23.269466 3 CGCAGAG 20710 0.0 23.209309 8 GAGTACT 9610 0.0 21.13389 12-13 GCAGAGT 21575 0.0 20.563587 9 AGAGTAC 16465 0.0 19.799253 10-11 CATGGGA 7355 0.0 19.54946 4 AGTACTT 10510 0.0 19.00809 12-13 GTACTTT 10815 0.0 18.758247 14-15 CATGGGG 7630 0.0 18.533886 4 GTCTAGG 1455 0.0 17.285936 1 >>END_MODULE