FastQCFastQC Report
Fri 27 May 2016
SRR939653_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939653_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4147123
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT113450.27356314244839136No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85690.20662517123316576No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63790.15381747780328675No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA89800.035.1656841
GTATCAA142900.033.5594521
TCAACGC172500.027.328134
ATCAACG172400.027.3166923
CAACGCA175650.026.8380455
TATCAAC176700.026.7870082
AACGCAG180200.026.1228586
ACGCAGA205250.022.568447
TACATGG133950.022.3748172
CGCAGAG205450.022.203478
GTACATG140300.022.1055151
ACATGGG137900.020.9817713
GCAGAGT220950.019.4126389
GAGTACT103650.019.19516612-13
AGAGTAC178100.018.9526810-11
AGTACTT113950.018.1403712-13
CATGGGA72750.018.107414
GTACTTT115400.017.07787914-15
CAGAGTA208100.016.6832310-11
GAGTACA121650.016.48051
Produced by FastQC (version 0.11.5)