FastQCFastQC Report
Fri 27 May 2016
SRR939652_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939652_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4155100
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44350.10673629996871316No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA118700.043.2812961
GGTATCA76050.041.64481
TATCAAC157700.031.0923522
GTACATG115950.030.5172211
ATCAACG171750.028.576693
TCAACGC172900.028.355884
CAACGCA175950.027.9236555
AACGCAG180750.027.3645636
TACATGG133050.025.7756542
ACGCAGA193100.025.174487
ACATGGG137250.023.8514083
CGCAGAG204600.023.2286598
GCAGAGT210500.021.0532479
GAGTACT97850.020.61975312-13
CATGGGA75300.019.7586084
AGAGTAC166050.019.58306510-11
CATGGGG74700.018.715954
GTACTTT105300.018.46851514-15
AGTACTT108400.018.00339512-13
CAGAGTA201700.016.92906410-11
Produced by FastQC (version 0.11.5)