##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939652_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4155100 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 14.777584414334191 2.0 2.0 31.0 2.0 33.0 2 22.21390363649491 16.0 16.0 31.0 16.0 34.0 3 26.69192919544656 28.0 25.0 31.0 16.0 34.0 4 31.928503285119493 35.0 32.0 35.0 22.0 37.0 5 32.53321171572285 35.0 33.0 35.0 25.0 37.0 6 32.90298043368391 35.0 33.0 37.0 26.0 37.0 7 33.06026690091694 35.0 33.0 37.0 26.0 37.0 8 32.97928425308657 35.0 33.0 37.0 26.0 37.0 9 34.31998315323337 37.0 33.0 39.0 26.0 39.0 10-11 34.1411205506486 37.0 33.0 39.0 25.5 39.0 12-13 34.12800714784241 37.0 33.0 39.0 25.5 39.0 14-15 34.789989049601694 38.0 33.0 40.0 25.0 41.0 16-17 34.366990445476645 38.0 32.5 40.0 24.5 41.0 18-19 34.59838150706361 38.0 33.0 40.0 25.0 41.0 20-21 34.58341423792448 38.0 33.0 40.0 24.5 41.0 22-23 34.390920916464104 38.0 32.5 40.0 23.5 41.0 24-25 34.178443840100115 38.0 32.0 40.0 23.0 41.0 26-27 34.26799499410363 38.0 33.0 40.0 23.0 41.0 28-29 33.99453081754952 38.0 32.0 40.0 20.0 41.0 30-31 33.86008664051407 38.0 32.0 40.0 20.0 41.0 32-33 33.545224302664195 38.0 32.0 40.0 18.0 41.0 34-35 33.27078168997136 37.0 31.0 40.0 17.5 41.0 36-37 32.991537989458735 37.0 31.0 40.0 16.0 41.0 38-39 32.778397631825946 37.0 30.5 40.0 15.5 41.0 40-41 32.37477581766985 37.0 30.0 40.0 14.0 41.0 42-43 32.11848583668263 37.0 30.0 39.5 13.0 41.0 44-45 31.778968015210225 36.5 30.0 39.0 9.0 40.5 46-47 31.446691776371207 36.0 30.0 39.0 9.0 40.0 48-49 31.069586050877234 36.0 29.0 39.0 8.0 40.0 50-51 30.42266828716517 35.5 28.0 38.5 7.0 40.0 52-53 30.46748489807706 35.0 28.0 38.5 4.0 40.0 54-55 30.56546713677168 35.5 28.0 39.0 2.0 40.0 56-57 30.249500012033405 35.0 28.0 39.0 2.0 40.0 58-59 29.801300690717433 35.0 27.0 38.0 2.0 40.0 60-61 29.194361988881134 34.0 26.0 38.0 2.0 40.0 62-63 28.764003393420133 34.0 26.0 38.0 2.0 40.0 64-65 28.16637686216938 33.5 25.0 37.5 2.0 39.5 66-67 27.549373661283724 33.0 23.5 37.0 2.0 39.0 68-69 27.169788693412915 32.5 22.5 36.0 2.0 39.0 70-71 26.62686950975909 32.0 21.5 36.0 2.0 39.0 72-73 26.01075160645953 31.5 20.0 35.5 2.0 38.0 74-75 25.422472022334 31.0 18.5 35.0 2.0 37.5 76-77 24.763830353060094 31.0 15.5 35.0 2.0 37.0 78-79 24.07153197275637 30.5 9.5 34.0 2.0 36.0 80-81 23.316993092825683 30.0 7.0 34.0 2.0 36.0 82-83 22.598697624605904 29.0 2.0 34.0 2.0 35.0 84-85 21.882038218093427 29.0 2.0 34.0 2.0 35.0 86-87 21.171496233544318 28.5 2.0 33.5 2.0 35.0 88-89 20.338746600563162 26.5 2.0 33.0 2.0 34.5 90-91 19.38403756828957 25.5 2.0 32.0 2.0 34.0 92-93 18.333303169598807 24.5 2.0 32.0 2.0 34.0 94-95 17.322923515679527 21.0 2.0 31.5 2.0 34.0 96-97 16.350073283434817 17.5 2.0 31.0 2.0 34.0 98-99 15.250821039204833 2.0 2.0 31.0 2.0 34.0 100-101 13.736549421193232 2.0 2.0 29.5 2.0 33.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 14.936034539656596 1101 2 8.16942691734312 1101 3 4.0372851177824565 1101 4 2.7224723769959525 1101 5 2.001085584968557 1101 6 1.750597642752453 1101 7 1.331690790182762 1101 8 1.4264841950638996 1101 9 1.5115177657941175 1101 10-11 1.4723822631954775 1101 12-13 1.1358649179730662 1101 14-15 1.3319889620685643 1101 16-17 1.5558746574985989 1101 18-19 1.4972633649261482 1101 20-21 1.434115247217882 1101 22-23 1.338253280541295 1101 24-25 1.6409468700278396 1101 26-27 1.6661536012982694 1101 28-29 1.7347734584663144 1101 30-31 1.6447251562570457 1101 32-33 1.636865640562533 1101 34-35 1.715824166644687 1101 36-37 1.7554691537867768 1101 38-39 1.6292454904636955 1101 40-41 1.7649858073597429 1101 42-43 1.6926456620813184 1101 44-45 1.565897792085078 1101 46-47 1.3382368304589676 1101 48-49 1.2779758865305908 1101 50-51 1.3921229756389053 1101 52-53 1.5187888420531621 1101 54-55 1.5964017294052155 1101 56-57 1.2260361721154105 1101 58-59 1.3258589244951366 1101 60-61 1.4727151523144961 1101 62-63 1.397847764299069 1101 64-65 1.256121332146435 1101 66-67 1.2590384194843587 1101 68-69 0.9864145310511923 1101 70-71 0.7263267940283242 1101 72-73 0.9048138055390602 1101 74-75 0.659069809285775 1101 76-77 0.631028923235128 1101 78-79 0.608749261716806 1101 80-81 0.4946283073722988 1101 82-83 0.6165207127126067 1101 84-85 0.5746892762897602 1101 86-87 0.31708750832116905 1101 88-89 0.5401465886131263 1101 90-91 0.5311078915917342 1101 92-93 0.5713176356316367 1101 94-95 0.25676490022819465 1101 96-97 -0.3936082654765709 1101 98-99 -0.6206659900681473 1101 100-101 -0.6390422059042198 1102 1 14.962230078157077 1102 2 8.216505395012348 1102 3 3.9753263856998515 1102 4 2.584056857017451 1102 5 1.861674364765861 1102 6 1.652646519841106 1102 7 1.397789979391959 1102 8 1.4101096699167073 1102 9 1.601915077226188 1102 10-11 1.4846227039635025 1102 12-13 1.2076791870754633 1102 14-15 1.2747266123490348 1102 16-17 1.4311168219008508 1102 18-19 1.4426364787591623 1102 20-21 1.4320031247671565 1102 22-23 1.4357734117733472 1102 24-25 1.2606905725342443 1102 26-27 1.4263517150536913 1102 28-29 1.5310442873756713 1102 30-31 1.553904666688176 1102 32-33 1.4981522362031683 1102 34-35 1.5577368342922782 1102 36-37 1.4455069757656815 1102 38-39 1.4000496846403152 1102 40-41 1.494048256516301 1102 42-43 1.3205309373330323 1102 44-45 1.4505480989300956 1102 46-47 1.0950538415052122 1102 48-49 0.9817934448573027 1102 50-51 0.9299233703280905 1102 52-53 1.2289365612302312 1102 54-55 1.108913621506069 1102 56-57 1.095656714350877 1102 58-59 0.9159478780744905 1102 60-61 1.1648796709404259 1102 62-63 0.7991494783132147 1102 64-65 0.612915944370851 1102 66-67 0.6269560250691448 1102 68-69 0.38969512167964027 1102 70-71 0.5669374539612306 1102 72-73 0.6814623078436668 1102 74-75 0.582809994700586 1102 76-77 0.38804806120522173 1102 78-79 0.41530346065913193 1102 80-81 0.3299721963397566 1102 82-83 0.30990467041263514 1102 84-85 0.2839724075940673 1102 86-87 0.13296262618929333 1102 88-89 0.18977387709443505 1102 90-91 0.3783369545119193 1102 92-93 0.4606201866368913 1102 94-95 0.24636377290579858 1102 96-97 -0.2650688692628016 1102 98-99 -0.38619958963085566 1102 100-101 -0.45196854270713693 1103 1 14.86083837139482 1103 2 7.9490202372169705 1103 3 3.952196143131726 1103 4 2.448905249239921 1103 5 1.8412454347854137 1103 6 1.3288039473338458 1103 7 1.1459342395465626 1103 8 1.1736535981588858 1103 9 1.3141326438658965 1103 10-11 1.3233060059742527 1103 12-13 0.9204575361986329 1103 14-15 1.090729425191192 1103 16-17 1.356854564724621 1103 18-19 1.2154925730020167 1103 20-21 1.0329339093679337 1103 22-23 1.2826979693678808 1103 24-25 1.3770025335459906 1103 26-27 1.342772866518274 1103 28-29 1.4766487025769166 1103 30-31 1.357080340829647 1103 32-33 1.2715579178261294 1103 34-35 1.3462354118292765 1103 36-37 1.426990933340612 1103 38-39 1.3212285179609253 1103 40-41 1.3680796364173702 1103 42-43 1.3281222974105944 1103 44-45 1.19543371473954 1103 46-47 1.1317623220146373 1103 48-49 0.9405211026578115 1103 50-51 0.9091833745226978 1103 52-53 1.1746575108721302 1103 54-55 1.1210387350310071 1103 56-57 1.0930689069405837 1103 58-59 1.1159711849626213 1103 60-61 1.0859387298257772 1103 62-63 0.8994730141662366 1103 64-65 0.8222903498779353 1103 66-67 0.8725068407496472 1103 68-69 0.865777349687491 1103 70-71 0.6437789049035239 1103 72-73 0.6906150292310507 1103 74-75 0.6006402382644147 1103 76-77 0.6198424392403119 1103 78-79 0.6674031483921361 1103 80-81 0.5953302054721128 1103 82-83 0.39735207252436666 1103 84-85 0.33238525013195996 1103 86-87 0.3319725259796833 1103 88-89 0.0897729446081783 1103 90-91 0.2733211162065139 1103 92-93 0.33522335973484374 1103 94-95 0.19029369039973076 1103 96-97 -0.44134720387185133 1103 98-99 -0.6297706368914184 1103 100-101 -0.4012814610187583 1104 1 14.831131704164314 1104 2 8.166005036386778 1104 3 3.9033687372350414 1104 4 2.42582645102037 1104 5 1.7747001467731138 1104 6 1.4493336252728568 1104 7 1.251606140571937 1104 8 1.1995223281094596 1104 9 1.3252641788278368 1104 10-11 1.1475146430415109 1104 12-13 1.0500939839930155 1104 14-15 1.081442778360021 1104 16-17 1.3204366017256532 1104 18-19 1.2454987032216067 1104 20-21 1.2447258716863132 1104 22-23 1.1885238689864153 1104 24-25 1.3258964816663479 1104 26-27 1.3041972225043068 1104 28-29 1.3410176323993142 1104 30-31 1.333358566691082 1104 32-33 1.3823936976548694 1104 34-35 1.4078277244940693 1104 36-37 1.3623153546431368 1104 38-39 1.088290898652616 1104 40-41 1.2754476670081019 1104 42-43 1.160514647399097 1104 44-45 0.9036858238124061 1104 46-47 0.915011715312886 1104 48-49 0.8707905059672782 1104 50-51 0.9805127925440722 1104 52-53 1.031548063086781 1104 54-55 0.9432515650397555 1104 56-57 1.0843955118944315 1104 58-59 1.0816366692522692 1104 60-61 0.945226135082013 1104 62-63 0.6993191994336954 1104 64-65 0.732978979921711 1104 66-67 0.6779455298969488 1104 68-69 0.8724398457823703 1104 70-71 0.7812868856036737 1104 72-73 0.6301183726598616 1104 74-75 0.519629409333394 1104 76-77 0.5686026287577128 1104 78-79 0.38907371213791464 1104 80-81 0.14841536373704045 1104 82-83 0.1754935112919611 1104 84-85 0.21156806014433727 1104 86-87 0.008354924205551839 1104 88-89 0.05755274944932154 1104 90-91 0.16227267092427766 1104 92-93 0.32603806607189156 1104 94-95 0.391718288468919 1104 96-97 -0.1486303674635252 1104 98-99 -0.3032648145617607 1104 100-101 -0.22756151782556167 1105 1 14.721355665091288 1105 2 7.9172573314588135 1105 3 3.9587985650339554 1105 4 2.4693172426613685 1105 5 1.7825114987937383 1105 6 1.365498411191453 1105 7 1.1899289235156374 1105 8 1.136303645518808 1105 9 1.1274467545821878 1105 10-11 1.0655529059635782 1105 12-13 0.9824988810114448 1105 14-15 1.1253933951185147 1105 16-17 1.3817534830989828 1105 18-19 1.220094619548938 1105 20-21 1.2588515219525007 1105 22-23 1.1526642488571994 1105 24-25 1.2537825771830953 1105 26-27 1.249728239190567 1105 28-29 1.4826845828148194 1105 30-31 1.2374467742719943 1105 32-33 1.0857523565849192 1105 34-35 1.0425522276853272 1105 36-37 1.175178312469349 1105 38-39 1.1340014675467316 1105 40-41 1.391107668820034 1105 42-43 1.2373450148477474 1105 44-45 1.2253239929757562 1105 46-47 1.2109025913894413 1105 48-49 1.1354762054922602 1105 50-51 1.1546304755483874 1105 52-53 1.31762467506897 1105 54-55 1.1931023942247805 1105 56-57 1.265887523047379 1105 58-59 1.1731365220068923 1105 60-61 1.2007918177685326 1105 62-63 1.180927537474222 1105 64-65 1.1700881032371306 1105 66-67 1.1487311300895762 1105 68-69 1.062770937094566 1105 70-71 1.135987798119654 1105 72-73 1.1804813836889778 1105 74-75 1.0868393232744005 1105 76-77 1.078697981539598 1105 78-79 1.1874847338749035 1105 80-81 1.1572297932164375 1105 82-83 1.0940091309558362 1105 84-85 0.876900068573299 1105 86-87 0.8373893107132098 1105 88-89 0.8766390792209968 1105 90-91 1.0161735890746968 1105 92-93 1.2358997010205037 1105 94-95 1.0719359886805755 1105 96-97 0.6918358541633651 1105 98-99 0.4044656031712446 1105 100-101 0.3083847217018256 1106 1 14.686503871700658 1106 2 7.937914367318541 1106 3 3.6260828476726488 1106 4 2.4102710939835887 1106 5 1.6840889714457106 1106 6 1.2530464201960498 1106 7 1.0050281331976194 1106 8 1.0353690149796932 1106 9 1.13276193075383 1106 10-11 0.9768041639859675 1106 12-13 0.730352334516617 1106 14-15 1.097029500355994 1106 16-17 1.1923949375719616 1106 18-19 1.175030029106857 1106 20-21 1.0100865770305845 1106 22-23 0.9942310974229329 1106 24-25 1.0365040957482634 1106 26-27 0.9733985557644829 1106 28-29 1.1082272076149806 1106 30-31 0.9613063627018548 1106 32-33 0.9093937126193623 1106 34-35 0.9456313429673813 1106 36-37 1.0628512646494315 1106 38-39 1.28156827689876 1106 40-41 1.306092516964931 1106 42-43 1.1600864212334017 1106 44-45 1.0818979697454658 1106 46-47 0.9955755227498742 1106 48-49 1.2822237969314614 1106 50-51 1.2703816991071761 1106 52-53 1.0044339250177785 1106 54-55 1.1311611517281932 1106 56-57 1.1657297636542303 1106 58-59 1.1856712913302019 1106 60-61 1.1793790083465687 1106 62-63 1.1370362506196479 1106 64-65 1.203946288867165 1106 66-67 1.1102328909671861 1106 68-69 1.1560826190010154 1106 70-71 1.2222290859545701 1106 72-73 1.002750866183188 1106 74-75 0.9412541202656151 1106 76-77 1.0026137021754487 1106 78-79 0.9179389974464343 1106 80-81 1.0717300791329158 1106 82-83 0.9696596439985399 1106 84-85 0.9922665328446563 1106 86-87 0.9739601099594175 1106 88-89 0.8376020052835429 1106 90-91 1.0757044010471688 1106 92-93 1.4607654546949185 1106 94-95 1.4636175070355257 1106 96-97 1.014239325264171 1106 98-99 0.920729364101815 1106 100-101 0.7845176947945482 1107 1 14.569435400247055 1107 2 7.867970213465711 1107 3 3.70681235339881 1107 4 2.1246944086550883 1107 5 1.3979442090426772 1107 6 1.2406663813115912 1107 7 0.8713118281214207 1107 8 0.9197555255829357 1107 9 0.8057002897626191 1107 10-11 0.7848801405868073 1107 12-13 0.48849665555750477 1107 14-15 0.7070307774593445 1107 16-17 0.8973837133962306 1107 18-19 0.7803410371206567 1107 20-21 0.6804603273506089 1107 22-23 0.7336466678511542 1107 24-25 0.7995242043030473 1107 26-27 0.743462192938928 1107 28-29 0.9434581542880025 1107 30-31 0.8411133677979237 1107 32-33 0.7673772062326449 1107 34-35 0.7205739280634802 1107 36-37 0.7250390113852063 1107 38-39 0.6426790562085287 1107 40-41 0.9824648954191346 1107 42-43 0.863555140462978 1107 44-45 0.8399598962185557 1107 46-47 0.6696322928310607 1107 48-49 0.7305302762551271 1107 50-51 0.7593720578229153 1107 52-53 0.6949844650642767 1107 54-55 0.6889971364196796 1107 56-57 0.6421916438609401 1107 58-59 0.7652404048194015 1107 60-61 0.7702719872842181 1107 62-63 0.5291661335243028 1107 64-65 0.39937152148312904 1107 66-67 0.43768438194305403 1107 68-69 0.4438980426270511 1107 70-71 0.31807591696642135 1107 72-73 0.37938283107488857 1107 74-75 0.3836156983871142 1107 76-77 0.09594474906574746 1107 78-79 0.2411911277105787 1107 80-81 0.27776116739220313 1107 82-83 0.24763552376647624 1107 84-85 0.35644435102256367 1107 86-87 0.4746360141744752 1107 88-89 0.45682719374338276 1107 90-91 0.8611429740678211 1107 92-93 0.9658959412125689 1107 94-95 0.9687948273123439 1107 96-97 0.6146151450291519 1107 98-99 0.4148393096611773 1107 100-101 0.32686593947919107 1108 1 14.213571611225714 1108 2 7.458006863231432 1108 3 3.310473291403028 1108 4 -0.044798083077893125 1108 5 0.15723036174643568 1108 6 0.4503704342605914 1108 7 0.37541140861153366 1108 8 0.5165852591608058 1108 9 0.5240396015905873 1108 10-11 0.39468864209100474 1108 12-13 0.1829703183094864 1108 14-15 0.17476297874699043 1108 16-17 0.4194954527719048 1108 18-19 0.31374630965943595 1108 20-21 0.2880713031573734 1108 22-23 -0.45234742330907096 1108 24-25 0.02841890215452736 1108 26-27 0.11722683925480482 1108 28-29 0.15312353240401677 1108 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1.6841233622782958 2201 74-75 1.7034694481122052 2201 76-77 1.930417984204876 2201 78-79 2.023581220762779 2201 80-81 1.9162271196078784 2201 82-83 1.7710686444272952 2201 84-85 1.7532108447308694 2201 86-87 1.787153104278584 2201 88-89 1.9556975622763453 2201 90-91 1.7906220755072546 2201 92-93 2.1445603573892242 2201 94-95 2.2030363108740083 2201 96-97 1.858023223316284 2201 98-99 1.7700679147063312 2201 100-101 1.300016482592934 2202 1 -14.017598347532697 2202 2 -6.928095874554643 2202 3 -1.9393928988132458 2202 4 0.03874074626396862 2202 5 0.42082154793542514 2202 6 0.7340127549281874 2202 7 0.9972246512010017 2202 8 1.124078578444852 2202 9 1.3353992000511141 2202 10-11 1.256268898570049 2202 12-13 1.2226867831606043 2202 14-15 1.3703249467050043 2202 16-17 1.4795594907709742 2202 18-19 1.4359314116282818 2202 20-21 1.5836691889174972 2202 22-23 1.4815364319440008 2202 24-25 1.660989440660778 2202 26-27 1.5238315910169788 2202 28-29 1.831688237199394 2202 30-31 1.8072697657616672 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1.725960517195567 2202 100-101 1.261641173071002 2203 1 -14.017598347532697 2203 2 -6.905671146812994 2203 3 -1.960135671818641 2203 4 0.11222118504460354 2203 5 0.5526566630177783 2203 6 0.9686209319637555 2203 7 1.0542576502423415 2203 8 1.1388328931493916 2203 9 1.1259297091056553 2203 10-11 1.2498067866200913 2203 12-13 1.1557352188946268 2203 14-15 1.3669726317195554 2203 16-17 1.4933013145366516 2203 18-19 1.5517733929093112 2203 20-21 1.4619171535194297 2203 22-23 1.4975608614339677 2203 24-25 1.7915588478054332 2203 26-27 1.729614035078292 2203 28-29 1.70661355644841 2203 30-31 1.6749903603043421 2203 32-33 1.8680755840217742 2203 34-35 1.7724101594724644 2203 36-37 2.020338182746478 2203 38-39 1.9271748596526734 2203 40-41 2.056261482525308 2203 42-43 1.9758923857537667 2203 44-45 1.9704316180642785 2203 46-47 1.8228331672088132 2203 48-49 1.7661807995858396 2203 50-51 1.9083775198252155 2203 52-53 1.843390841084826 2203 54-55 1.7383757526616037 2203 56-57 1.733136078564666 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1.4236010714168685 2204 18-19 1.482723377101074 2204 20-21 1.6123676882739275 2204 22-23 1.6524665934643608 2204 24-25 1.7089609003323858 2204 26-27 1.658927775888614 2204 28-29 1.8063987824698557 2204 30-31 1.8282015220098913 2204 32-33 1.8907189756335967 2204 34-35 1.9577948084024115 2204 36-37 1.9864763939808583 2204 38-39 2.008130294960644 2204 40-41 2.1068464781462453 2204 42-43 2.1451615364335197 2204 44-45 2.1040228497621563 2204 46-47 2.0765391085620735 2204 48-49 2.1573780645791345 2204 50-51 1.9518105601307738 2204 52-53 1.928264357018442 2204 54-55 1.883067455699333 2204 56-57 1.9164380376958299 2204 58-59 1.8758272122183755 2204 60-61 1.9951906972313829 2204 62-63 1.6635768107208229 2204 64-65 2.0114844913448984 2204 66-67 2.1075219371954717 2204 68-69 2.056550470875983 2204 70-71 2.1639052146233695 2204 72-73 2.207793140548606 2204 74-75 2.1509738694286042 2204 76-77 2.3368593072795 2204 78-79 2.2895149057654223 2204 80-81 2.307045232135483 2204 82-83 2.1208021736387117 2204 84-85 2.2473669743225066 2204 86-87 2.296066640145483 2204 88-89 2.556422454255067 2204 90-91 2.9426620076712133 2204 92-93 3.066744956031272 2204 94-95 2.9632177920154668 2204 96-97 2.4834164025058065 2204 98-99 2.3219902399472474 2204 100-101 2.184844565684818 2205 1 -14.017598347532697 2205 2 -6.850701189731449 2205 3 -1.553216263139575 2205 4 0.4484558059315802 2205 5 0.7426623854784395 2205 6 1.1582318046390014 2205 7 1.2377874547249306 2205 8 1.4250837276749024 2205 9 1.6423312350951633 2205 10-11 1.5994496097006774 2205 12-13 1.54758023558513 2205 14-15 1.866917791471586 2205 16-17 2.0055035081465746 2205 18-19 2.0226579899527124 2205 20-21 2.1453458611970646 2205 22-23 2.1595193735942004 2205 24-25 2.173388127729133 2205 26-27 1.9895706397516335 2205 28-29 2.290954996601009 2205 30-31 2.307062844806012 2205 32-33 2.3069825940360786 2205 34-35 2.2964340030585433 2205 36-37 2.3797621217068965 2205 38-39 2.414489594988872 2205 40-41 2.613337305129022 2205 42-43 2.3015437925253437 2205 44-45 2.4519399633194006 2205 46-47 2.3947182411239396 2205 48-49 2.472894199681221 2205 50-51 2.4335010296010893 2205 52-53 2.299867235934613 2205 54-55 2.3299737498003736 2205 56-57 2.637181381378209 2205 58-59 2.4787296489480752 2205 60-61 2.536223437846683 2205 62-63 2.591855200033084 2205 64-65 2.657298302615949 2205 66-67 2.8674386744238127 2205 68-69 2.7524185675925104 2205 70-71 2.828105421604274 2205 72-73 2.9568033955114394 2205 74-75 2.8590601428315594 2205 76-77 2.9392191718010956 2205 78-79 3.084743525579139 2205 80-81 3.019660812152413 2205 82-83 2.979197789406893 2205 84-85 3.1301141753762245 2205 86-87 3.388749394873116 2205 88-89 3.6445302724882076 2205 90-91 4.021073899664817 2205 92-93 4.203500342238819 2205 94-95 4.37280834615979 2205 96-97 3.8192890151692787 2205 98-99 3.643682214733211 2205 100-101 3.160552440367292 2206 1 -14.017598347532697 2206 2 -6.890563004762855 2206 3 -1.8613297567501448 2206 4 0.11816037599872686 2206 5 0.5249643131370902 2206 6 1.031546944340107 2206 7 1.153104327152917 2206 8 1.2601610506118774 2206 9 1.3606691168365543 2206 10-11 1.5378000537119405 2206 12-13 1.2859300298238097 2206 14-15 1.4942548068495327 2206 16-17 1.7144773006525256 2206 18-19 1.7801422871153676 2206 20-21 1.6425680834192846 2206 22-23 1.8700571686992404 2206 24-25 1.998690055387783 2206 26-27 1.9169977569272874 2206 28-29 2.036665631212223 2206 30-31 1.974110494795184 2206 32-33 2.0847456436353866 2206 34-35 2.0523993485209573 2206 36-37 2.1641931375180263 2206 38-39 2.0475599870183245 2206 40-41 2.305530720766882 2206 42-43 2.1487657981567025 2206 44-45 2.18266814179027 2206 46-47 2.260805960422182 2206 48-49 2.280180749388819 2206 50-51 2.3194715732441438 2206 52-53 2.2955969305415067 2206 54-55 2.229304267452534 2206 56-57 2.2878066791906413 2206 58-59 2.137815635519445 2206 60-61 2.3071025801468785 2206 62-63 2.1958928868426995 2206 64-65 2.2928213695425264 2206 66-67 2.49396780589446 2206 68-69 2.417951164516925 2206 70-71 2.5304972344697703 2206 72-73 2.691498002399033 2206 74-75 2.595685224053131 2206 76-77 2.700880640287128 2206 78-79 2.8414784183666626 2206 80-81 2.8656987155528917 2206 82-83 2.756791248362923 2206 84-85 2.9654650525692077 2206 86-87 3.082625288310414 2206 88-89 3.1718469283253263 2206 90-91 3.5116262063574766 2206 92-93 3.8234962270124804 2206 94-95 3.797586557119285 2206 96-97 3.4984053244610216 2206 98-99 3.2232312725629697 2206 100-101 2.9930135623079455 2207 1 -14.017598347532697 2207 2 -6.942945915469846 2207 3 -2.0590953067401365 2207 4 -0.1693247847857151 2207 5 0.2648902335797061 2207 6 0.71458623501875 2207 7 0.7708310552875233 2207 8 1.0430387206425564 2207 9 1.2275323602709705 2207 10-11 1.0930670499257147 2207 12-13 0.9909881118157884 2207 14-15 1.2488024609511896 2207 16-17 1.3946821298062062 2207 18-19 1.5235581305996888 2207 20-21 1.396154581309581 2207 22-23 1.269574225914873 2207 24-25 1.6107327128205569 2207 26-27 1.6709321024801866 2207 28-29 1.5865147715658452 2207 30-31 1.7150403412898427 2207 32-33 1.8254509484664538 2207 34-35 1.8594241577702135 2207 36-37 1.9236707011724334 2207 38-39 1.8653165992082776 2207 40-41 2.0843373051290186 2207 42-43 2.055646464817883 2207 44-45 2.0622789225317852 2207 46-47 1.9741008008988956 2207 48-49 1.77617971304268 2207 50-51 1.7195530689822434 2207 52-53 1.8807701895211153 2207 54-55 2.0035982223742153 2207 56-57 2.0590111985371458 2207 58-59 2.093954684109825 2207 60-61 2.0487297669606015 2207 62-63 2.0205274925787897 2207 64-65 2.0929241816595514 2207 66-67 2.1865736955208597 2207 68-69 2.04385597968815 2207 70-71 2.076866321744923 2207 72-73 2.21911703826812 2207 74-75 2.0891009304546238 2207 76-77 2.2225567245437112 2207 78-79 2.198646479165639 2207 80-81 2.247095411308532 2207 82-83 2.2969432183801715 2207 84-85 2.564600813913497 2207 86-87 2.816175555210666 2207 88-89 2.9747243653152076 2207 90-91 3.1656449263877455 2207 92-93 3.458413141113642 2207 94-95 3.4836494150768083 2207 96-97 2.9671652458303157 2207 98-99 2.7445246901199916 2207 100-101 2.5480123559791057 2208 1 -14.017598347532697 2208 2 -6.994547018664923 2208 3 -2.324404811772684 2208 4 -0.34552589065412675 2208 5 0.23693341680543512 2208 6 0.6262388444137272 2208 7 0.7726627333826812 2208 8 0.8444299672619024 2208 9 1.05058584028113 2208 10-11 0.9488472396431931 2208 12-13 0.7921723979692743 2208 14-15 0.9358823579387874 2208 16-17 1.2504182095427936 2208 18-19 1.1868366829012018 2208 20-21 1.0162041270658975 2208 22-23 1.2318421472654322 2208 24-25 1.4928954608278104 2208 26-27 1.298122675419826 2208 28-29 1.4834545859474773 2208 30-31 1.4589601814245796 2208 32-33 1.5764426433145076 2208 34-35 1.5480529165866415 2208 36-37 1.6342431729799216 2208 38-39 1.6362347551437466 2208 40-41 1.7198001703173347 2208 42-43 1.777192742425612 2208 44-45 1.712384407763846 2208 46-47 1.714043948034643 2208 48-49 1.7050657355254017 2208 50-51 1.5411267482447641 2208 52-53 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14-15 0.08361678221620394 2312 16-17 -0.1136934594363268 2312 18-19 0.11804494869881665 2312 20-21 0.05818828713073998 2312 22-23 0.16572880571363413 2312 24-25 0.20093600922302102 2312 26-27 0.4244105541742229 2312 28-29 -0.397092629090821 2312 30-31 0.14308568095317042 2312 32-33 0.2900622647490678 2312 34-35 -0.5811983861326624 2312 36-37 0.1631095939174898 2312 38-39 0.30873209533769597 2312 40-41 0.49598094961067574 2312 42-43 0.4003378951252188 2312 44-45 0.5435271453109429 2312 46-47 0.5851317567743735 2312 48-49 0.5730000552228205 2312 50-51 0.2882532736827592 2312 52-53 0.3801845235026349 2312 54-55 0.18804672315044968 2312 56-57 0.16472479796348338 2312 58-59 0.25026353109309696 2312 60-61 0.3023388278285424 2312 62-63 0.16308100348408416 2312 64-65 0.14718854027931627 2312 66-67 0.11348792794690254 2312 68-69 0.40210193115699866 2312 70-71 0.20001505371440942 2312 72-73 0.06803101283156465 2312 74-75 0.43379359708950105 2312 76-77 0.21946069545115776 2312 78-79 0.3717318051940417 2312 80-81 0.3621970197706652 2312 82-83 0.23553456444061283 2312 84-85 0.293138918411433 2312 86-87 0.2586566084910835 2312 88-89 0.8959284865247632 2312 90-91 1.0780312504857115 2312 92-93 1.681455460605406 2312 94-95 1.673657283418521 2312 96-97 1.140992191765715 2312 98-99 0.9945116645262431 2312 100-101 0.9505863457670394 2313 1 -14.017598347532697 2313 2 -7.323246033251717 2313 3 -3.6795510667522606 2313 4 -1.303264840991332 2313 5 -0.7022223026122987 2313 6 -1.2077904595912017 2313 7 -0.6201848200072675 2313 8 -0.26056718599869555 2313 9 -2.6733843708699716 2313 10-11 -0.7233161958023615 2313 12-13 -0.9422511023690774 2313 14-15 -1.1036356636848907 2313 16-17 -1.0861041373060658 2313 18-19 -0.37440880273790356 2313 20-21 -1.2834978691116916 2313 22-23 -2.3208736110728907 2313 24-25 -1.6691602864695625 2313 26-27 -0.6689206435922017 2313 28-29 -0.0972159758837634 2313 30-31 -1.4403963024319566 2313 32-33 -0.5863893866402492 2313 34-35 -1.3931194520979346 2313 36-37 -1.4681072334705902 2313 38-39 -0.8795792981008148 2313 40-41 -0.4118294665575988 2313 42-43 -0.3982268438955785 2313 44-45 -0.501667262053413 2313 46-47 -1.0852458420518687 2313 48-49 -0.5395220994156098 2313 50-51 -1.4707085902266783 2313 52-53 -0.5068599222254377 2313 54-55 -1.278785544465535 2313 56-57 -0.9502328173195416 2313 58-59 -0.8032489269813468 2313 60-61 -2.1527669003175696 2313 62-63 -0.9796243210759279 2313 64-65 -0.784745385684829 2313 66-67 -1.9539003299870252 2313 68-69 -0.7880115941382151 2313 70-71 -0.48869378024407695 2313 72-73 -1.3042121474417208 2313 74-75 -0.7823945935683589 2313 76-77 -0.3145714184110453 2313 78-79 -0.09412961031668132 2313 80-81 -0.220187749074217 2313 82-83 -0.06749051137025575 2313 84-85 -0.11008851313459189 2313 86-87 -0.12981288195948437 2313 88-89 0.13448889462641134 2313 90-91 0.6908995665415247 2313 92-93 1.1068595104807848 2313 94-95 1.2680683755653739 2313 96-97 0.9180791853016004 2313 98-99 0.6827021685244326 2313 100-101 0.7941817199304069 2314 1 -14.017598347532697 2314 2 -7.167153238738631 2314 3 -3.589363284685838 2314 4 -1.5422387441317866 2314 5 -1.2873807070437415 2314 6 -6.440586953282416 2314 7 -2.2020021523726605 2314 8 -4.294159195856992 2314 9 -1.7279408392605617 2314 10-11 -0.5692812732735533 2314 12-13 -3.0159132160413975 2314 14-15 -3.1634307509872244 2314 16-17 -2.9628817896988053 2314 18-19 -1.4867188583785165 2314 20-21 -2.2728311561244325 2314 22-23 -1.5460982858903805 2314 24-25 -3.252712843964993 2314 26-27 -5.114176297299487 2314 28-29 -2.287965155490248 2314 30-31 -2.117943914805309 2314 32-33 -4.354220616779301 2314 34-35 -3.0531469010394723 2314 36-37 -1.1359886176255927 2314 38-39 -1.5226096868024257 2314 40-41 -2.585075453637348 2314 42-43 -1.380173993701952 2314 44-45 -2.6282155077410074 2314 46-47 -2.2627692958426877 2314 48-49 -1.4224408235549433 2314 50-51 -2.5138897604284836 2314 52-53 -1.0943938540527114 2314 54-55 -0.5202759333428446 2314 56-57 -1.2440239418157084 2314 58-59 -1.6712492272665394 2314 60-61 -0.797363803386947 2314 62-63 -2.923737533384749 2314 64-65 -1.1129974303794015 2314 66-67 -2.2914396525723895 2314 68-69 -2.3878374526863198 2314 70-71 -2.8272841009981775 2314 72-73 -3.002391788265495 2314 74-75 -2.23039993321407 2314 76-77 -1.0986595776708121 2314 78-79 -0.7174977080499261 2314 80-81 -0.5364621282785116 2314 82-83 -0.5252808755698695 2314 84-85 -0.21290863831198692 2314 86-87 0.02072024404928996 2314 88-89 0.2042560857539435 2314 90-91 0.5353873766398927 2314 92-93 1.1023262822472688 2314 94-95 1.077477125204993 2314 96-97 0.8597326146623026 2314 98-99 1.0782103093677726 2314 100-101 1.0677019967677968 2315 1 -14.017598347532697 2315 2 -7.271449177872068 2315 3 -4.6541537190198525 2315 4 -2.963294299955752 2315 5 -3.7042651875838253 2315 6 -2.934454345297464 2315 7 -3.2959154960021593 2315 8 -3.766763229828978 2315 9 -2.518895081437382 2315 10-11 -4.778951350344499 2315 12-13 -4.527650739990186 2315 14-15 -3.582844314980484 2315 16-17 -4.614556071127748 2315 18-19 -1.5610360662381737 2315 20-21 -3.19913034944814 2315 22-23 -4.225169741187678 2315 24-25 -5.140146956274819 2315 26-27 -4.774135558187094 2315 28-29 -2.599818122133989 2315 30-31 -3.39932696177312 2315 32-33 -4.3775764910973365 2315 34-35 -3.809960462563371 2315 36-37 -5.205995043336166 2315 38-39 -3.7127920653245567 2315 40-41 -4.690757993473262 2315 42-43 -3.355624638930255 2315 44-45 -2.0076918311179774 2315 46-47 -1.3314962160269914 2315 48-49 -1.1605650689902482 2315 50-51 -0.9389295788981329 2315 52-53 -0.6819743566109153 2315 54-55 -2.337689528471543 2315 56-57 -4.38755912405734 2315 58-59 -3.724644627488459 2315 60-61 -4.59468846388987 2315 62-63 -2.478721109440304 2315 64-65 -3.1797354401484164 2315 66-67 -1.9146007156651343 2315 68-69 -1.3644265545308798 2315 70-71 -2.0593565295463705 2315 72-73 -2.1279537695316186 2315 74-75 -2.7448785836967495 2315 76-77 -2.3875478760599798 2315 78-79 -1.5701124676440728 2315 80-81 -1.3297966211385628 2315 82-83 -0.9088438596118849 2315 84-85 -0.6554731463800927 2315 86-87 -1.3232899952158306 2315 88-89 -2.0057268219632967 2315 90-91 -1.4720970730868395 2315 92-93 -0.545323743600516 2315 94-95 -0.41585479092901423 2315 96-97 -1.335091896873644 2315 98-99 -0.879045160672689 2315 100-101 -0.593319224181629 2316 1 -14.017598347532697 2316 2 -7.329211649982495 2316 3 -5.327521237570071 2316 4 -3.2953712512511224 2316 5 -2.4024729004350895 2316 6 -1.64804992478917 2316 7 -4.945567264010538 2316 8 -3.3083630412697715 2316 9 -1.6110169750396608 2316 10-11 -4.7260654995508276 2316 12-13 -4.160761001048989 2316 14-15 -2.3220315344233526 2316 16-17 -3.7421970962234923 2316 18-19 -2.807193242032419 2316 20-21 -3.6075016515876825 2316 22-23 -3.4627916445369067 2316 24-25 -3.879711825780859 2316 26-27 -4.533352186840652 2316 28-29 -4.27574049386854 2316 30-31 -3.2206675131670224 2316 32-33 -3.4854413948063225 2316 34-35 -4.623788684063726 2316 36-37 -2.834066795075998 2316 38-39 -3.6097628457363733 2316 40-41 -3.428493936154098 2316 42-43 -7.175142864843323 2316 44-45 -4.133745299349119 2316 46-47 -5.299026063850498 2316 48-49 -4.914553034163504 2316 50-51 -3.7734826988972827 2316 52-53 -5.200747826826891 2316 54-55 -5.295257770422776 2316 56-57 -5.28988230992816 2316 58-59 -4.175640411488928 2316 60-61 -3.7754237030180313 2316 62-63 -2.915803563332794 2316 64-65 -2.963759344989814 2316 66-67 -3.2707272948927866 2316 68-69 -3.2921109535604387 2316 70-71 -3.596079143714185 2316 72-73 -4.499400695608969 2316 74-75 -5.000275515002006 2316 76-77 -4.658492125270033 2316 78-79 -4.823561913751405 2316 80-81 -4.767393581153023 2316 82-83 -4.30687984890087 2316 84-85 -3.962275410862734 2316 86-87 -4.059507229952548 2316 88-89 -3.794492042714026 2316 90-91 -3.0740144313440148 2316 92-93 -2.8458543764966535 2316 94-95 -2.243694210790469 2316 96-97 -2.406354871395113 2316 98-99 -2.537089984244007 2316 100-101 -2.331326220720575 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 37466.0 3 11819.0 4 13210.0 5 15346.0 6 17668.0 7 20924.0 8 25969.0 9 31027.0 10 36314.0 11 41589.0 12 46093.0 13 50211.0 14 54038.0 15 58810.0 16 63487.0 17 66773.0 18 64917.0 19 67529.0 20 72479.0 21 78948.0 22 85386.0 23 94755.0 24 104715.0 25 115516.0 26 127215.0 27 141956.0 28 158011.0 29 176322.0 30 198597.0 31 223230.0 32 252372.0 33 288644.0 34 330988.0 35 376899.0 36 376800.0 37 205782.0 38 23190.0 39 105.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.781317926709875 18.680758791628854 13.623777910746604 23.914145370914667 2 15.383385030740223 20.13346819543571 40.76464788634006 23.718498887484007 3 19.42214426899614 24.74008250951129 29.409549291613846 26.428223929878726 4 12.790977503076881 16.389916123630837 37.63961711633505 33.179489256957226 5 13.929117299998579 37.26699603347773 34.54968369749948 14.25420296902421 6 31.843965374701668 36.09263621888734 18.057815204800146 14.005583201610847 7 28.011660849017606 32.43758169958272 21.58141707662592 17.969340374773747 8 25.790465244983 35.394543249252294 20.171518954389526 18.64347255137518 9 26.599437043085555 16.51108910399519 20.211449875553214 36.67802397736604 10-11 24.945682375400814 26.384316231716 27.930886485803917 20.739114907079266 12-13 25.914974612407654 23.722031320703106 28.034710535654998 22.328283531234238 14-15 23.047124455771208 25.02341899630394 26.090575627058467 25.83888092086639 16-17 22.62746507268896 27.95491185232906 26.648224188423708 22.769398886558275 18-19 22.504963216219547 27.03341217996701 28.56791462243703 21.893709981376414 20-21 23.44441300754634 26.25578386351655 28.368805526325403 21.930997602611704 22-23 22.889563589234896 26.25377047195813 28.263278144206165 22.593387794600808 24-25 22.747641564901695 26.337948109905085 28.556823092432175 22.35758723276104 26-27 22.6835141942985 26.468756938082127 28.47336445774814 22.37436440987123 28-29 22.62728829502424 26.708798630037865 28.402776102843635 22.261136972094256 30-31 22.748336882403066 26.56321595772717 28.324254107362222 22.364193052507535 32-33 22.600568713703133 26.54014969679296 28.32831264636635 22.530968943137555 34-35 22.821409945284355 26.631198468564882 28.27265574363535 22.274735842515412 36-37 22.93408323492965 26.39734238912495 28.365568829570442 22.303005546374962 38-39 22.646179806343035 26.69363038026938 28.343824602678662 22.31636521070892 40-41 22.89634352183181 26.598129804492714 28.281992978327334 22.22353369534814 42-43 22.693380234944474 26.648648172936067 28.064750360195067 22.593221231924392 44-45 22.723458150098438 26.779697557521853 27.99248427451521 22.504360017864496 46-47 22.892042887498352 26.80087523340804 27.672924571061092 22.634157308032517 48-49 22.85703775311011 26.83071798029334 27.69903408532397 22.61321018127258 50-51 22.768318368505916 27.013297721881102 27.63627297090052 22.58211093871246 52-53 22.86727807787952 27.08256464521298 27.48007041565264 22.57008686125486 54-55 22.785661642866682 27.04027054614839 27.45267197739272 22.721395833592208 56-57 22.741600338099598 27.279844478044275 27.364670907706724 22.61388427614941 58-59 22.823134287331857 27.03988555489864 27.49633739025432 22.640642767515185 60-61 22.803321072407226 27.054314009684553 27.59706707169079 22.545297846217434 62-63 22.742266022492093 26.935948134204 27.65350632864382 22.668279514660085 64-65 22.692253855903754 27.019725092680456 27.54902458405767 22.738996467358117 66-67 22.65887328046641 27.19362616247271 27.28122458861898 22.866275968441897 68-69 22.65710409853972 27.022276276366064 27.472028668414637 22.84859095667958 70-71 22.701672719801376 26.84554492621269 27.390538058785562 23.062244295200372 72-73 22.73101134078258 26.83684111760906 27.3023949799345 23.129752561673858 74-75 22.55909246862066 27.17413910996606 27.146150924840622 23.120617496572653 76-77 22.6005855805318 27.02034331377779 27.1834710424757 23.19560006321471 78-79 22.6202779247146 27.05265780059604 27.195389916709438 23.131674357979918 80-81 22.587146819096315 26.970005914924734 27.1298524429556 23.312994823023352 82-83 22.676847278518256 26.987025099861206 27.199859686470674 23.136267935149863 84-85 22.662994121330744 26.989252848620378 26.970862788080296 23.376890241968578 86-87 22.46735234382915 26.855030898889954 27.271864401873714 23.405752355407188 88-89 22.629100529611037 26.754004887975057 27.09907460385307 23.517819978560837 90-91 22.3869009410134 26.844996100666595 27.175923910480904 23.5921790478391 92-93 22.43324553569383 26.809014448383454 27.1754636750029 23.582276340919815 94-95 22.372681387403734 26.743495338647726 27.17757773745874 23.7062455364898 96-97 22.385074573553382 26.589855834278204 27.09704481267434 23.92802477949407 98-99 22.336760759144724 26.65795568163659 26.892901231140748 24.112382328077935 100-101 22.386546398732662 26.62188038652516 26.991576594049377 23.9999966206928 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 4553.0 1 2953.5 2 1250.5 3 1023.0 4 857.0 5 806.0 6 988.0 7 2078.0 8 2914.5 9 2632.0 10 2372.0 11 2355.5 12 2434.5 13 2656.0 14 2972.5 15 3256.0 16 3618.0 17 4080.0 18 4520.0 19 5047.0 20 5824.0 21 7056.0 22 8860.0 23 10909.5 24 13540.0 25 17570.0 26 23299.0 27 29484.5 28 34854.0 29 41006.0 30 48184.5 31 56412.0 32 64563.5 33 71603.5 34 79607.0 35 87348.5 36 95200.5 37 102848.0 38 109399.5 39 115526.5 40 121449.0 41 126884.5 42 131214.0 43 136697.0 44 142765.0 45 147932.0 46 153487.0 47 159650.5 48 164168.0 49 164007.0 50 161244.5 51 158140.5 52 155278.0 53 151532.5 54 144990.0 55 135597.0 56 124114.0 57 112467.5 58 100924.5 59 87917.0 60 73368.0 61 59218.5 62 46320.0 63 34844.0 64 25403.0 65 18273.5 66 13073.5 67 9391.5 68 6605.0 69 4686.0 70 3236.0 71 2101.0 72 1330.0 73 840.0 74 551.0 75 383.5 76 252.0 77 155.5 78 98.5 79 61.5 80 46.5 81 39.5 82 28.5 83 17.0 84 13.5 85 14.0 86 15.5 87 13.5 88 12.0 89 12.0 90 7.0 91 3.5 92 4.5 93 4.5 94 5.5 95 5.0 96 5.5 97 7.5 98 4.5 99 1.5 100 0.5 >>END_MODULE >>Per base N content fail #Base N-Count 1 54.2281533537099 2 0.089023128203894 3 0.12112825202762871 4 0.1935934153209309 5 0.12314986402252653 6 0.1498399557170706 7 0.1568433972708238 8 0.15412384780149696 9 0.14288464778224352 10-11 0.13077904262231954 12-13 0.14265601309234435 14-15 0.14980385550287598 16-17 0.1439917210175447 18-19 0.1523068036870352 20-21 0.17957449880869292 22-23 0.19827440976149793 24-25 0.2261197083102693 26-27 0.21784072585497338 28-29 0.2166012851676253 30-31 0.23218454429496282 32-33 0.2226901879617819 34-35 0.2084787369738394 36-37 0.1941469519385815 38-39 0.1906452311617049 40-41 0.1910904671367717 42-43 0.18518206541358812 44-45 0.20042838920844264 46-47 0.2277442179490265 48-49 0.22977786334865585 50-51 0.24046352675025873 52-53 0.2679839233712787 54-55 0.2549758128564896 56-57 0.23046376741835337 58-59 0.18733604486053285 60-61 0.19702293566941828 62-63 0.2021130658708575 64-65 0.19062116435224183 66-67 0.2006209236841472 68-69 0.1881543163822772 70-71 0.2009698924213617 72-73 0.2208851772520517 74-75 0.25255709850545116 76-77 0.2525811653149142 78-79 0.24722630020938122 80-81 0.252689465957498 82-83 0.24300257514861257 84-85 0.2127144954393396 86-87 0.22430266419580758 88-89 0.22661307790426224 90-91 0.2136771678178624 92-93 0.212341459892662 94-95 0.2498134822266612 96-97 0.2809559336718731 98-99 0.29934297610165816 100-101 0.2945536810185074 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 4155100.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 70.74431457973694 #Duplication Level Percentage of deduplicated Percentage of total 1 82.43215212258569 58.31606101244932 2 10.64267096559497 15.058169255173665 3 3.263196359995014 6.925577694808192 4 1.3637958657222036 3.85923214988785 5 0.7243922607226527 2.562331698584508 6 0.4146057625075506 1.7598600293643523 7 0.27102422749525235 1.3421396246058026 8 0.17101864385735466 0.9678877392035755 9 0.13182419615831184 0.8393231162020107 >10 0.5608000704006151 6.487677319520419 >50 0.01796609265749245 0.8474485696021858 >100 0.00637074314441434 0.7554646889893827 >500 7.307566635191699E-5 0.032491488500320895 >1k 1.096134921031565E-4 0.24633561310851135 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4435 0.10673629996871316 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 2.406680946306948E-5 2 0.0 0.0 0.0 0.0 2.406680946306948E-5 3 0.0 0.0 0.0 4.813361892613896E-5 2.406680946306948E-5 4 0.0 0.0 0.0 4.813361892613896E-5 4.813361892613896E-5 5 0.0 0.0 0.0 4.813361892613896E-5 4.813361892613896E-5 6 0.0 0.0 0.0 4.813361892613896E-5 4.813361892613896E-5 7 0.0 0.0 0.0 4.813361892613896E-5 4.813361892613896E-5 8 0.0 0.0 0.0 4.813361892613896E-5 7.220042838920844E-5 9 0.0 0.0 0.0 4.813361892613896E-5 7.220042838920844E-5 10-11 0.0 0.0 0.0 1.564342615099516E-4 8.423383312074317E-5 12-13 0.0 0.0 0.0 2.406680946306948E-4 4.0913576087218116E-4 14-15 2.406680946306948E-5 0.0 0.0 2.7676830882529904E-4 4.933695939929243E-4 16-17 2.406680946306948E-5 0.0 0.0 3.489687372145075E-4 5.776034271136676E-4 18-19 3.6100214194604216E-5 0.0 0.0 3.6100214194604224E-4 5.776034271136676E-4 20-21 4.813361892613896E-5 2.406680946306948E-5 0.0 4.813361892613896E-4 7.220042838920844E-4 22-23 4.813361892613896E-5 2.406680946306948E-5 0.0 8.062381170128275E-4 7.821713075497581E-4 24-25 1.203340473153474E-4 2.406680946306948E-5 0.0 0.0011792736636904045 7.942047122812928E-4 26-27 1.203340473153474E-4 2.406680946306948E-5 0.0 0.0015763760198310509 8.182715217443623E-4 28-29 1.203340473153474E-4 2.406680946306948E-5 0.0 0.0028398835166421987 8.182715217443623E-4 30-31 1.203340473153474E-4 7.220042838920844E-5 0.0 0.007087675386873962 8.182715217443623E-4 32-33 1.203340473153474E-4 8.423383312074317E-5 0.0 0.016184929363914226 8.664051406705013E-4 34-35 1.203340473153474E-4 9.626723785227792E-5 1.203340473153474E-5 0.02884407114148877 8.904719501335708E-4 36-37 1.444008567784169E-4 9.626723785227792E-5 2.406680946306948E-5 0.048951890447883326 8.904719501335708E-4 38-39 1.6846766624148638E-4 9.626723785227792E-5 2.406680946306948E-5 0.07968520613222305 9.626723785227792E-4 40-41 1.6846766624148638E-4 9.626723785227792E-5 2.406680946306948E-5 0.11532815094702895 9.626723785227792E-4 42-43 1.6846766624148638E-4 9.626723785227792E-5 2.406680946306948E-5 0.15215036942552523 9.867391879858486E-4 44-45 1.9253447570455584E-4 9.626723785227792E-5 2.406680946306948E-5 0.1958075617915333 0.0010348728069119878 46-47 1.9253447570455584E-4 9.626723785227792E-5 2.406680946306948E-5 0.2492840124184737 0.001070973021106592 48-49 2.1660128516762532E-4 9.626723785227792E-5 2.406680946306948E-5 0.30883733243483913 0.0011191066400327307 50-51 2.1660128516762532E-4 9.626723785227792E-5 2.406680946306948E-5 0.3740824528892205 0.0011552068542273351 52-53 2.1660128516762532E-4 9.626723785227792E-5 2.406680946306948E-5 0.43852133522658904 0.0011913070684219393 54-55 2.1660128516762532E-4 9.626723785227792E-5 2.406680946306948E-5 0.5122981396356285 0.0012274072826165435 56-57 2.406680946306948E-4 9.626723785227792E-5 2.406680946306948E-5 0.6003224952468051 0.0012394406873480783 58-59 2.406680946306948E-4 1.203340473153474E-4 2.406680946306948E-5 0.69640923202811 0.0012635074968111479 60-61 2.406680946306948E-4 1.203340473153474E-4 2.406680946306948E-5 0.8036750018050107 0.0013357079252003562 62-63 2.406680946306948E-4 1.203340473153474E-4 2.406680946306948E-5 0.9129744169815408 0.0013718081393949604 64-65 2.406680946306948E-4 1.203340473153474E-4 2.406680946306948E-5 1.0315997208250103 0.0013718081393949604 66-67 2.406680946306948E-4 1.203340473153474E-4 2.406680946306948E-5 1.1683112319799764 0.00139587494885803 68-69 2.406680946306948E-4 1.203340473153474E-4 2.406680946306948E-5 1.3226637144713727 0.00139587494885803 70-71 2.7676830882529904E-4 1.203340473153474E-4 2.406680946306948E-5 1.495788308343963 0.0014079083535895646 72-73 3.008351182883685E-4 1.203340473153474E-4 2.406680946306948E-5 1.6789367283579217 0.0014801087819787731 74-75 3.1286852301990327E-4 1.203340473153474E-4 2.406680946306948E-5 1.8647685976270125 0.0015162089961733773 76-77 3.1286852301990327E-4 1.203340473153474E-4 2.406680946306948E-5 2.0695410459435393 0.0015402758056364467 78-79 3.1286852301990327E-4 1.203340473153474E-4 2.406680946306948E-5 2.302375394094005 0.0015643426150995163 80-81 3.1286852301990327E-4 1.203340473153474E-4 2.406680946306948E-5 2.5581574450675078 0.0016004428292941205 82-83 3.1286852301990327E-4 1.203340473153474E-4 2.406680946306948E-5 2.830822362879353 0.0016846766624148636 84-85 3.1286852301990327E-4 1.203340473153474E-4 2.406680946306948E-5 3.1058939616375056 0.0016846766624148636 86-87 3.1286852301990327E-4 1.203340473153474E-4 2.406680946306948E-5 3.4016028495102404 0.0017568770908040722 88-89 3.24901927751438E-4 1.203340473153474E-4 4.813361892613896E-5 3.7185627301388653 0.0018411109239248153 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 11870 0.0 43.281296 1 GGTATCA 7605 0.0 41.6448 1 TATCAAC 15770 0.0 31.092352 2 GTACATG 11595 0.0 30.517221 1 ATCAACG 17175 0.0 28.57669 3 TCAACGC 17290 0.0 28.35588 4 CAACGCA 17595 0.0 27.923655 5 AACGCAG 18075 0.0 27.364563 6 TACATGG 13305 0.0 25.775654 2 ACGCAGA 19310 0.0 25.17448 7 ACATGGG 13725 0.0 23.851408 3 CGCAGAG 20460 0.0 23.228659 8 GCAGAGT 21050 0.0 21.053247 9 GAGTACT 9785 0.0 20.619753 12-13 CATGGGA 7530 0.0 19.758608 4 AGAGTAC 16605 0.0 19.583065 10-11 CATGGGG 7470 0.0 18.71595 4 GTACTTT 10530 0.0 18.468515 14-15 AGTACTT 10840 0.0 18.003395 12-13 CAGAGTA 20170 0.0 16.929064 10-11 >>END_MODULE