FastQCFastQC Report
Fri 27 May 2016
SRR939652_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939652_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4155100
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT115380.2776828475848957No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86510.2082019686650141No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62970.1515486991889485No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA87450.035.6707081
GTATCAA136850.033.2776451
TCAACGC169400.026.5948724
ATCAACG169800.026.5047573
CAACGCA172350.026.2217795
TATCAAC172800.026.1829992
AACGCAG177750.025.462616
TACATGG140900.023.84042
GTACATG145950.023.701181
ACATGGG144300.022.2962173
ACGCAGA200500.022.220757
CGCAGAG201200.021.8622578
GAGTACT106200.019.62228212-13
AGAGTAC178000.019.48090710-11
GCAGAGT221400.018.9947599
CATGGGG76950.018.8207844
AGTACTT115450.018.80561312-13
GTACTTT114450.018.10484714-15
CATGGGA74550.017.465034
CAGAGTA209350.016.23711410-11
Produced by FastQC (version 0.11.5)