##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939652_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4155100 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.477784168852736 31.0 31.0 34.0 30.0 34.0 2 31.675173642030277 31.0 31.0 34.0 30.0 34.0 3 31.736110562922672 31.0 31.0 34.0 30.0 34.0 4 35.53657529301341 37.0 35.0 37.0 33.0 37.0 5 35.284611682029315 37.0 35.0 37.0 33.0 37.0 6 35.28353589564632 37.0 35.0 37.0 32.0 37.0 7 35.18991384082212 37.0 35.0 37.0 32.0 37.0 8 35.14778248417607 37.0 35.0 37.0 32.0 37.0 9 36.75250703954177 39.0 35.0 39.0 32.0 39.0 10-11 36.63073993405695 39.0 35.0 39.0 32.0 39.0 12-13 36.566036918485715 39.0 35.0 39.0 32.0 39.0 14-15 37.64976727395249 39.0 36.0 41.0 32.0 41.0 16-17 37.59866669875574 39.0 36.0 41.0 32.0 41.0 18-19 37.553131693581385 39.0 36.0 41.0 32.0 41.0 20-21 37.48441650020457 39.0 36.0 41.0 32.0 41.0 22-23 37.34640044764265 39.0 36.0 41.0 31.5 41.0 24-25 37.22043705325985 39.0 36.0 40.5 31.0 41.0 26-27 36.93680886139924 39.0 36.0 40.0 31.0 41.0 28-29 36.74108108108108 39.0 35.5 40.0 30.0 41.0 30-31 36.506259055137065 38.0 35.0 40.0 30.0 41.0 32-33 36.350787345671584 38.0 35.0 40.0 30.0 41.0 34-35 36.337531828355516 38.0 35.0 40.0 30.0 41.0 36-37 36.27884936583957 38.0 35.0 40.0 30.0 41.0 38-39 36.34160513585714 38.0 35.0 40.0 30.0 41.0 40-41 36.241661933527475 38.0 35.0 40.0 30.0 41.0 42-43 36.07017989940074 38.0 35.0 40.0 29.5 41.0 44-45 35.87321436307189 38.0 35.0 40.0 29.0 41.0 46-47 35.614134557531706 38.0 34.0 40.0 27.5 41.0 48-49 35.335953286322834 38.0 34.0 40.0 27.0 41.0 50-51 35.09997665519482 38.0 34.0 40.0 26.5 41.0 52-53 34.869278236384204 38.0 33.5 40.0 26.0 41.0 54-55 34.58378486679021 38.0 33.0 40.0 26.0 41.0 56-57 33.8392400904912 37.5 32.5 40.0 23.5 41.0 58-59 30.795935597217877 35.5 27.5 39.0 8.5 40.5 60-61 30.534550793001372 34.5 26.5 39.0 10.5 40.0 62-63 32.235623089697 35.5 30.5 39.0 20.0 40.0 64-65 32.25515089889534 35.5 31.0 39.0 19.0 40.0 66-67 27.451228369954997 32.0 19.0 37.0 4.5 39.5 68-69 19.868288127842895 20.5 8.0 32.0 2.0 37.0 70-71 17.90866381073861 16.0 7.5 29.0 2.0 35.0 72-73 19.643171042814856 20.0 7.5 31.0 2.0 35.5 74-75 21.13005294698082 24.0 9.5 32.5 2.0 36.0 76-77 23.041452431951097 27.5 12.5 33.0 2.0 35.5 78-79 25.280202762869727 30.5 17.5 34.5 2.0 36.5 80-81 26.863976919929726 32.0 23.5 35.0 2.0 36.5 82-83 27.904704940915984 33.0 26.5 35.0 2.0 36.0 84-85 27.92683196553633 34.0 27.5 35.0 2.0 36.0 86-87 27.642913768621693 33.5 27.0 35.0 2.0 36.0 88-89 27.203575244879787 33.0 26.0 35.0 2.0 35.0 90-91 25.44642138576689 32.5 16.5 34.5 2.0 35.0 92-93 23.89544776299006 31.5 4.5 34.0 2.0 35.0 94-95 20.113073692570577 28.5 2.0 34.0 2.0 35.0 96-97 17.388725421770836 19.5 2.0 33.0 2.0 35.0 98-99 17.655741257731464 22.5 2.0 33.0 2.0 35.0 100-101 16.878105581093116 18.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.26196961965743526 1101 2 0.25859331683813025 1101 3 0.25355553353817584 1101 4 0.2338080774657385 1101 5 0.3454747083930556 1101 6 0.36017805046859763 1101 7 0.2930215911092162 1101 8 0.3596152246278024 1101 9 0.44541481259538074 1101 10-11 0.37246593255513716 1101 12-13 0.34797527504111514 1101 14-15 0.4133501919442537 1101 16-17 0.536492142921901 1101 18-19 0.49697304157685096 1101 20-21 0.43874875283480463 1101 22-23 0.4420398813799693 1101 24-25 0.42195333203700613 1101 26-27 0.49204643508676327 1101 28-29 0.42209193642300846 1101 30-31 0.323864802227547 1101 32-33 0.37668552434392666 1101 34-35 0.33591068930315515 1101 36-37 0.22279742948752812 1101 38-39 0.2195860097208211 1101 40-41 0.33841260254017413 1101 42-43 0.47445737748623884 1101 44-45 0.3570934566447619 1101 46-47 0.325970924595282 1101 48-49 0.1784851674086596 1101 50-51 0.2909992463617499 1101 52-53 0.1302209002547059 1101 54-55 0.22810838670550737 1101 56-57 -3.2270832738411386 1101 58-59 -10.3470142949025 1101 60-61 -12.071424475812048 1101 62-63 -4.115111900118979 1101 64-65 -1.0443718925299947 1101 66-67 -2.0858911890765945 1101 68-69 -4.527357832659533 1101 70-71 -4.51298921913148 1101 72-73 -3.337881893547067 1101 74-75 -6.051085849635571 1101 76-77 -4.834028914435539 1101 78-79 1.3709940711391866 1101 80-81 2.164685277988813 1101 82-83 0.896839759416153 1101 84-85 0.5064192847589553 1101 86-87 0.5644285241924507 1101 88-89 0.4528069777383159 1101 90-91 1.7928720346707046 1101 92-93 1.0741879334522153 1101 94-95 -8.694224826287698 1101 96-97 -9.75587498747665 1101 98-99 -8.866159662507494 1101 100-101 -9.75680801112426 1102 1 0.25698297250282565 1102 2 0.28101790673244054 1102 3 0.2753777602625789 1102 4 0.2059800627350512 1102 5 0.29759082627935385 1102 6 0.287373234755151 1102 7 0.3684544170314723 1102 8 0.33569071164978226 1102 9 0.32003743511727123 1102 10-11 0.28072319560308756 1102 12-13 0.3315417327112229 1102 14-15 0.49091459105070356 1102 16-17 0.4575645577254761 1102 18-19 0.4371487088533783 1102 20-21 0.37998448508454175 1102 22-23 0.3207210296626073 1102 24-25 0.32050808712622825 1102 26-27 0.2078461162498968 1102 28-29 0.2948452658787559 1102 30-31 0.35281361698518765 1102 32-33 0.20662729073208652 1102 34-35 0.23792091429327655 1102 36-37 0.03459293611319225 1102 38-39 0.17041436159755818 1102 40-41 0.08299341208080335 1102 42-43 0.23794202792053198 1102 44-45 0.09574068417822446 1102 46-47 0.14468147174718382 1102 48-49 0.20984847762868242 1102 50-51 0.22958790557558473 1102 52-53 0.10461154368717729 1102 54-55 -0.19220769380878266 1102 56-57 -3.703730340892193 1102 58-59 -9.950631981703797 1102 60-61 -8.18385941283157 1102 62-63 -2.6017013675080207 1102 64-65 -0.8850229501400726 1102 66-67 -2.0577975416085614 1102 68-69 -5.109819577333802 1102 70-71 -4.917822792892874 1102 72-73 -3.7096093492049373 1102 74-75 -4.2307424106395 1102 76-77 -4.354445590153354 1102 78-79 1.5471841623259976 1102 80-81 1.9291821977906132 1102 82-83 0.5395846469210923 1102 84-85 0.21833001065811075 1102 86-87 0.11201587959758541 1102 88-89 0.010322574899110037 1102 90-91 1.4692635600914308 1102 92-93 0.8944020887982944 1102 94-95 -8.931376647075844 1102 96-97 -9.899061755746708 1102 98-99 -8.916653046154869 1102 100-101 -9.788766729907218 1103 1 0.14433584052762427 1103 2 0.13587921309932227 1103 3 0.2496555746812703 1103 4 0.22844139129194474 1103 5 0.23924879632631502 1103 6 0.20013240205651783 1103 7 0.23459612027714627 1103 8 0.3447587453729142 1103 9 0.2890755723827212 1103 10-11 0.34533951464993606 1103 12-13 0.3018449956808311 1103 14-15 0.3520439172535177 1103 16-17 0.3318329050379347 1103 18-19 0.3325691634820487 1103 20-21 0.4064459952918682 1103 22-23 0.35071750570747895 1103 24-25 0.42117437753206133 1103 26-27 0.37732612556211365 1103 28-29 0.28842546678266245 1103 30-31 0.33591069675960483 1103 32-33 0.30349114452498327 1103 34-35 0.20163326430457573 1103 36-37 0.2819224034744039 1103 38-39 0.2988918438720205 1103 40-41 0.2527640199348795 1103 42-43 0.1074236651324938 1103 44-45 0.2113962170116679 1103 46-47 0.23615323693260137 1103 48-49 0.08540128524733603 1103 50-51 -0.011427023002553938 1103 52-53 0.1227660501272112 1103 54-55 0.05022748509397701 1103 56-57 0.4427380676061219 1103 58-59 -8.805479458825356 1103 60-61 -7.846264617306051 1103 62-63 -2.316484035254266 1103 64-65 -0.43086963791464683 1103 66-67 -1.873810364190195 1103 68-69 -3.362757607089385 1103 70-71 -3.9035730067983714 1103 72-73 -2.9080809357428485 1103 74-75 -3.8386092071377576 1103 76-77 -0.4693485749681052 1103 78-79 3.4016944692757143 1103 80-81 2.8973450887045225 1103 82-83 1.3080693035446203 1103 84-85 0.8658349540206345 1103 86-87 0.7465374269579037 1103 88-89 0.6292653852009025 1103 90-91 1.9572683097654924 1103 92-93 1.3590952615711949 1103 94-95 -7.467057232706715 1103 96-97 -8.938617111528936 1103 98-99 -8.237474705629538 1103 100-101 -9.515463700796737 1104 1 0.21198980809430523 1104 2 0.22143676201046247 1104 3 0.24032480130067313 1104 4 0.10510937102989004 1104 5 0.3183722959661779 1104 6 0.16873375744638253 1104 7 0.23212396098888632 1104 8 0.19348190229657547 1104 9 0.20331102705395665 1104 10-11 0.2628621674277767 1104 12-13 0.2129181242516509 1104 14-15 0.3166693632878932 1104 16-17 0.2869876137927463 1104 18-19 0.28003826838571655 1104 20-21 0.326004444813627 1104 22-23 0.29968967120104395 1104 24-25 0.2689142631676873 1104 26-27 0.298775351761698 1104 28-29 0.2662012374153093 1104 30-31 0.2675824969680676 1104 32-33 0.31683720407774274 1104 34-35 0.23641019463809698 1104 36-37 0.08125201700091367 1104 38-39 0.032738883784951156 1104 40-41 0.051479232547471554 1104 42-43 0.12746792038435473 1104 44-45 0.13440105729577567 1104 46-47 -0.021665550957052915 1104 48-49 0.00398473720907333 1104 50-51 0.034140227620767405 1104 52-53 0.13098653517298686 1104 54-55 0.1424900478689679 1104 56-57 0.6087776163324818 1104 58-59 -8.402646197274272 1104 60-61 -7.876326978232729 1104 62-63 -2.3808017752149766 1104 64-65 -0.42542246053526256 1104 66-67 -1.7182857278626749 1104 68-69 -3.321145198458538 1104 70-71 -3.931418774702223 1104 72-73 -3.044384246321899 1104 74-75 -3.840905473097049 1104 76-77 -0.4047775669997371 1104 78-79 3.5123734313150834 1104 80-81 2.812333529233964 1104 82-83 1.211221103624382 1104 84-85 1.0034681897699436 1104 86-87 1.0098682797050031 1104 88-89 0.7415787149581483 1104 90-91 2.1290451526120044 1104 92-93 1.6168003623091955 1104 94-95 -6.537437546872425 1104 96-97 -7.960554901367971 1104 98-99 -5.878368939346725 1104 100-101 -5.1194904322926575 1105 1 0.22129489504204258 1105 2 0.2457923937458233 1105 3 0.2038093666815719 1105 4 0.1938398098413785 1105 5 0.20837787163073784 1105 6 0.25513578373733736 1105 7 0.19220642853359493 1105 8 0.1715730975500236 1105 9 0.22672197785372816 1105 10-11 0.2742569885251527 1105 12-13 0.24290606897980638 1105 14-15 0.33877253598493695 1105 16-17 0.30649060845766485 1105 18-19 0.3890983736849094 1105 20-21 0.34414579976699855 1105 22-23 0.45229503778325864 1105 24-25 0.4201614355598977 1105 26-27 0.37231253532348063 1105 28-29 0.43778043242392783 1105 30-31 0.47297474831440667 1105 32-33 0.37604469156087816 1105 34-35 0.3131324081789586 1105 36-37 0.31055466776223994 1105 38-39 0.31964999601753874 1105 40-41 0.23132456308547944 1105 42-43 0.3809747772762435 1105 44-45 0.3211689979744392 1105 46-47 0.29600091992291766 1105 48-49 0.1422760138984387 1105 50-51 0.16630678575350544 1105 52-53 0.11945766808681668 1105 54-55 0.2303173040116775 1105 56-57 0.7388538829517941 1105 58-59 -8.345708168424718 1105 60-61 -7.612860182622487 1105 62-63 -2.0979582278357114 1105 64-65 -0.17876466709995498 1105 66-67 -1.549130102572466 1105 68-69 -3.2598464060733043 1105 70-71 -3.7733482535914113 1105 72-73 -2.76664331366786 1105 74-75 -3.5420327261387854 1105 76-77 -0.07203035234921984 1105 78-79 3.7659484639309966 1105 80-81 3.1534406938717225 1105 82-83 1.6545375113398393 1105 84-85 1.1549611342538775 1105 86-87 0.9487445755504531 1105 88-89 0.9028009002254613 1105 90-91 2.30029144943639 1105 92-93 2.040476055836944 1105 94-95 -6.0985630497918955 1105 96-97 -7.79909127624558 1105 98-99 -5.88069375433742 1105 100-101 -5.257334297875971 1106 1 0.16097798962652732 1106 2 0.1671629877870764 1106 3 0.19414318654792595 1106 4 0.11520888788859196 1106 5 0.20058606652855104 1106 6 0.1835742957265225 1106 7 0.1365243211644298 1106 8 0.18217449440714972 1106 9 0.16411299051551254 1106 10-11 0.19491311984177173 1106 12-13 0.20928288769259495 1106 14-15 0.20367993199430856 1106 16-17 0.21926938207508329 1106 18-19 0.2362314723019452 1106 20-21 0.14574647218110215 1106 22-23 0.14148111372162475 1106 24-25 0.19238451852984184 1106 26-27 0.2346808687144275 1106 28-29 0.2944287181681844 1106 30-31 0.34731728696568354 1106 32-33 0.24623845985513526 1106 34-35 0.2843284456130917 1106 36-37 0.36318710045362224 1106 38-39 0.4642935140616089 1106 40-41 0.321942449915106 1106 42-43 0.43001862467506413 1106 44-45 0.4389372768704476 1106 46-47 0.3832795014133623 1106 48-49 0.415758205718582 1106 50-51 0.38033735102321486 1106 52-53 0.40540249318051735 1106 54-55 0.4791044582462902 1106 56-57 1.1170851737156156 1106 58-59 -8.02134888814592 1106 60-61 -7.312653163529653 1106 62-63 -1.6694457100707893 1106 64-65 0.10651953832411465 1106 66-67 1.0626189977861351 1106 68-69 -2.078478968331641 1106 70-71 -2.1045329433048234 1106 72-73 -0.7592671688649624 1106 74-75 -0.765872177114467 1106 76-77 2.5440386740935566 1106 78-79 4.80690369986111 1106 80-81 3.776039207712447 1106 82-83 2.2163920895104567 1106 84-85 1.837710729028668 1106 86-87 1.6508635208471247 1106 88-89 1.5429568232444986 1106 90-91 3.002559492746922 1106 92-93 2.606357644776576 1106 94-95 -5.9515679975921785 1106 96-97 -7.353602884616212 1106 98-99 -5.385994781231357 1106 100-101 -4.865585570451902 1107 1 0.02521130157858309 1107 2 0.08934414295742599 1107 3 0.08333675201744128 1107 4 0.08725425999649872 1107 5 0.09165825800606342 1107 6 0.022737610867849867 1107 7 0.07114021267016568 1107 8 0.03643022484855152 1107 9 0.02960553832377144 1107 10-11 0.01719833952544292 1107 12-13 0.044726452664406224 1107 14-15 0.14322128167456327 1107 16-17 0.06300140258996123 1107 18-19 0.04041392607637562 1107 20-21 0.07624136615664412 1107 22-23 0.13230585273663564 1107 24-25 0.1288543997940721 1107 26-27 0.22435258148222914 1107 28-29 0.11341581594034977 1107 30-31 0.013379370115366385 1107 32-33 0.08189317490480619 1107 34-35 -0.012294984684537269 1107 36-37 -0.03796271063860246 1107 38-39 0.11818305029490972 1107 40-41 0.03046092839626624 1107 42-43 -0.0905451781346045 1107 44-45 -0.1429360476157342 1107 46-47 -0.029227471391806148 1107 48-49 -0.07138938641899983 1107 50-51 -0.04172390773285173 1107 52-53 0.024300392798402015 1107 54-55 -0.10886932420537931 1107 56-57 0.40810970524862 1107 58-59 -8.796505335795832 1107 60-61 -8.023933017189332 1107 62-63 -2.5359829592837215 1107 64-65 -0.6161856871090201 1107 66-67 0.3128047650614718 1107 68-69 -2.638874385610979 1107 70-71 -2.476738246976712 1107 72-73 -1.1301154382356238 1107 74-75 -1.0978475553978626 1107 76-77 1.9602370280536512 1107 78-79 4.152868436479196 1107 80-81 3.0696974787434392 1107 82-83 1.4906251008504938 1107 84-85 0.9781958063297544 1107 86-87 0.8282683572365457 1107 88-89 0.7042531110245598 1107 90-91 2.1240979006101384 1107 92-93 1.6196372302744173 1107 94-95 -6.9162392922902995 1107 96-97 -8.382870027364712 1107 98-99 -6.282288389383794 1107 100-101 -5.50052530172467 1108 1 -0.08576484253141103 1108 2 -0.13396839094630764 1108 3 -0.15650894768812051 1108 4 -0.09928026677390989 1108 5 -0.07234129900444941 1108 6 -0.024519709735862705 1108 7 -0.11648067931557904 1108 8 -0.060151893412289326 1108 9 -0.04244300083260555 1108 10-11 -0.23180303050340711 1108 12-13 -0.1047371261971648 1108 14-15 -0.29079834480317146 1108 16-17 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56-57 0.13210472211709856 2313 58-59 3.2133272739630776 2313 60-61 3.178025273846888 2313 62-63 0.394317857596171 2313 64-65 -0.3138230739141079 2313 66-67 -1.9817775343942508 2313 68-69 -4.837132308834864 2313 70-71 -3.6131704990335543 2313 72-73 -3.02651208796852 2313 74-75 -3.5595965321528205 2313 76-77 0.4917568878305616 2313 78-79 3.0025829939236175 2313 80-81 2.2372162899996404 2313 82-83 0.7127464069181855 2313 84-85 0.419967907993545 2313 86-87 0.3655654103729127 2313 88-89 0.4661410480185566 2313 90-91 2.207691288904538 2313 92-93 3.5996382039940578 2313 94-95 5.54892362574228 2313 96-97 0.9954284246075922 2313 98-99 2.568912955878911 2313 100-101 3.9313201758698924 2314 1 -0.35420968533367514 2314 2 -0.2916575634542262 2314 3 -0.34720886072616963 2314 4 -0.1789461450699079 2314 5 -0.281501758983012 2314 6 -0.2940173029448516 2314 7 -0.3109500440494841 2314 8 -0.4559700900196688 2314 9 -0.3980267367191672 2314 10-11 -0.3550331970479732 2314 12-13 -0.3727966559313671 2314 14-15 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3.3765187185173744 2314 80-81 2.4934514005261548 2314 82-83 1.0389533577217307 2314 84-85 0.5874519733119641 2314 86-87 0.6354533145499914 2314 88-89 0.7115055950391422 2314 90-91 2.311581579390527 2314 92-93 3.8701763933222715 2314 94-95 6.423118306381792 2314 96-97 3.3742164304503746 2314 98-99 5.893293837295811 2314 100-101 6.298159067274469 2315 1 -0.41482609188521735 2315 2 -0.3342331299716008 2315 3 -0.43023177909387655 2315 4 -0.30674424140617873 2315 5 -0.38818412220583554 2315 6 -0.5057404401732271 2315 7 -0.4342128341391813 2315 8 -0.5731558327797401 2315 9 -0.6395247486139795 2315 10-11 -0.5034436254877264 2315 12-13 -0.5400587878822805 2315 14-15 -0.6761394371706686 2315 16-17 -0.6913734558821147 2315 18-19 -0.7189247068899078 2315 20-21 -0.7237575335930657 2315 22-23 -0.6548011228126001 2315 24-25 -0.5515180941742983 2315 26-27 -0.5335901441894464 2315 28-29 -0.5329583327292298 2315 30-31 -0.5057170723710698 2315 32-33 -0.6020600225165822 2315 34-35 -0.6026695437380667 2315 36-37 -0.3378326366039843 2315 38-39 -0.5673228635974326 2315 40-41 -0.6425427097647045 2315 42-43 -0.6170650657698928 2315 44-45 -0.7055087863850957 2315 46-47 -0.6506443229381347 2315 48-49 -0.7081419403162172 2315 50-51 -0.5140613584472788 2315 52-53 -0.4803046248695537 2315 54-55 -0.4599317804304661 2315 56-57 0.14501550426354726 2315 58-59 3.383118517780982 2315 60-61 3.3588460815157184 2315 62-63 0.6103940105404533 2315 64-65 0.025942764949782315 2315 66-67 -1.6811958145917565 2315 68-69 -4.82947859605256 2315 70-71 -3.6043788476795164 2315 72-73 -2.9288048990791253 2315 74-75 -0.5031862049149147 2315 76-77 2.0805253707419347 2315 78-79 3.6756441347525737 2315 80-81 2.7186912105140806 2315 82-83 1.0592551406551038 2315 84-85 0.7330368605989896 2315 86-87 0.6774963576849231 2315 88-89 0.7903263026832725 2315 90-91 2.421374668289186 2315 92-93 4.051058535483467 2315 94-95 6.599306915212768 2315 96-97 4.444399469258553 2315 98-99 6.643564661939404 2315 100-101 6.8984009574318605 2316 1 -0.5033273474697779 2316 2 -0.4604873410976573 2316 3 -0.6378020590440414 2316 4 -0.3568526983928848 2316 5 -0.5528286508875695 2316 6 -0.5684802373303128 2316 7 -0.43772554682209375 2316 8 -0.5296425175580097 2316 9 -0.7069830449531977 2316 10-11 -0.6902227719737724 2316 12-13 -0.5909566593253146 2316 14-15 -0.7197877033652347 2316 16-17 -0.7883110523547856 2316 18-19 -0.7429694170421541 2316 20-21 -0.6872678715641811 2316 22-23 -0.9495926178000076 2316 24-25 -0.86529306187645 2316 26-27 -0.8122050669492822 2316 28-29 -0.6557488317554245 2316 30-31 -0.7327108555882447 2316 32-33 -0.5944244822962332 2316 34-35 -0.6117303540818924 2316 36-37 -0.5529650888129112 2316 38-39 -0.65478407516121 2316 40-41 -0.6905351922069016 2316 42-43 -0.6747959045925356 2316 44-45 -0.7213095250267969 2316 46-47 -0.7564943545714229 2316 48-49 -0.7903231839917808 2316 50-51 -0.7900916998209624 2316 52-53 -0.6553343530713747 2316 54-55 -0.646835111938465 2316 56-57 0.1906964528723094 2316 58-59 3.393547578582467 2316 60-61 3.331059383800582 2316 62-63 0.6328656513422573 2316 64-65 0.005779911113293679 2316 66-67 -2.091465822867807 2316 68-69 -3.196515292438569 2316 70-71 -3.1196304785217066 2316 72-73 -0.6154649555405562 2316 74-75 5.600909198245578 2316 76-77 5.725958525263014 2316 78-79 4.807661851165911 2316 80-81 3.235714482689726 2316 82-83 1.4856968919873381 2316 84-85 1.083690039257064 2316 86-87 0.9561253249156714 2316 88-89 0.9098179460015565 2316 90-91 2.576411139609224 2316 92-93 4.287474652333469 2316 94-95 6.803430717445352 2316 96-97 3.877518413964985 2316 98-99 6.121696446039113 2316 100-101 6.20942577848669 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 8.0 3 15.0 4 46.0 5 150.0 6 325.0 7 844.0 8 2050.0 9 3627.0 10 5626.0 11 8723.0 12 12567.0 13 17243.0 14 21754.0 15 28901.0 16 38515.0 17 50775.0 18 56184.0 19 58038.0 20 61281.0 21 62734.0 22 62993.0 23 64289.0 24 68546.0 25 73968.0 26 92237.0 27 129259.0 28 181410.0 29 238987.0 30 293266.0 31 327249.0 32 344839.0 33 344158.0 34 353987.0 35 378623.0 36 377456.0 37 280754.0 38 107238.0 39 6435.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 41.98944410007041 19.611417704302404 14.284238970167848 24.11489922545934 2 15.605723736261778 20.91555325125964 39.630463786890495 23.848259225588084 3 19.305021070542395 24.859918798389447 29.868017299264277 25.967042831803884 4 13.168082597290077 17.122740728261654 37.094606627999326 32.61457004644895 5 13.65677455398801 37.61548992386762 34.6385608174087 14.089174704735669 6 31.91116940627181 36.715097109576185 17.46800317681885 13.905730307333156 7 27.83321701042093 32.57729055859065 21.43649972323169 18.15299270775673 8 25.63250042838962 36.025401097832635 19.868349612138925 18.473748861638818 9 25.98081875285793 16.43994127698491 20.198262376356766 37.38097759380039 10-11 24.80109985319246 26.61722942889461 27.90488796900195 20.676782748910977 12-13 25.609516016461697 23.79205073283435 28.172522923636013 22.425910327067943 14-15 22.615527110284063 25.187033349221537 26.107931398101286 26.089508142393115 16-17 22.27461700977317 28.162225718060423 26.73552101459905 22.827636257567356 18-19 22.243913181078128 27.182729113844843 28.564602168186692 22.00875553689034 20-21 23.1112728935525 26.39774012659142 28.39156698996414 22.099419989891942 22-23 22.57324733460085 26.373035546677574 28.33176096844841 22.721956150273158 24-25 22.52601926069079 26.51058519720969 28.520816010223594 22.442579531875925 26-27 22.534081651562722 26.627032594922618 28.36900239344708 22.46988336006758 28-29 22.413183543242106 26.83901720769789 28.364603246307695 22.383196002752307 30-31 22.636938326052157 26.626718562137796 28.244980222703823 22.491362889106224 32-33 22.483409821924546 26.64948681883516 28.231715183250106 22.635388175990187 34-35 22.70158422632836 26.678672210155174 28.240383631867438 22.379359931649027 36-37 22.80582547189126 26.457825207902886 28.291544808895058 22.444804511310792 38-39 22.565372210258037 26.727500373617417 28.280929952724904 22.426197463399642 40-41 22.71522798373555 26.642892781834764 28.217672793514165 22.42420644091552 42-43 22.51027194829557 26.713496827004928 28.073972961736658 22.70225826296284 44-45 22.602247908769428 26.792995211613725 27.999257136511762 22.60549974310509 46-47 22.776347632974865 26.808753810959544 27.697864297022107 22.717034259043484 48-49 22.685586033206995 26.87150310555416 27.752556130341517 22.69035473089733 50-51 22.680943188726975 27.014305381439307 27.679909428913028 22.62484200092069 52-53 22.800681622992844 27.044294056291328 27.50887938805638 22.64614493265945 54-55 22.791035552742688 26.99521651030504 27.440746933735312 22.773001003216965 56-57 22.589218669247067 27.165135878059672 27.606924408150995 22.638721044542265 58-59 21.749900043351918 26.63557739189684 29.181111750623582 22.433410814127658 60-61 22.030394361687254 26.87204622350068 28.610136396225442 22.48742301858662 62-63 22.710594455484102 26.878963400582506 27.725768450100908 22.684673693832483 64-65 22.613114852340853 26.963496796563845 27.634773741414392 22.78861460968091 66-67 22.406886343573742 26.64930763629019 28.44468968806674 22.499116332069327 68-69 21.728915314401185 26.089075538893958 30.078676255878474 22.103332890826387 70-71 22.49500480972681 26.05988362506756 29.14468934932024 22.300422215885384 72-73 21.851241686945695 26.30564294421493 29.271166938544763 22.57194843029461 74-75 22.00391097640566 26.552024007085688 28.76770355569241 22.676361460816246 76-77 20.983643324580353 27.01536741903494 28.858912157427547 23.14207709895716 78-79 22.28732224462683 26.8666719430844 27.990561021165938 22.85544479112284 80-81 22.347767676573124 27.016747240894468 27.423209711281032 23.212275371251373 82-83 22.65350562634955 27.03409455193772 27.30478651254894 23.007613309163794 84-85 22.63176952086119 27.003836417312577 27.145844386653973 23.218549675172262 86-87 22.469454588075262 26.893223487699164 27.378669727365036 23.258652196860538 88-89 22.636777126128642 26.85198355081311 27.18410560133465 23.327133721723598 90-91 22.308115349021403 26.847533629579907 27.613134416297463 23.231216605101228 92-93 22.235936901772018 26.977827488808998 27.388866548355484 23.3973690610635 94-95 21.12126634418777 26.910870912912838 28.486584915913287 23.48127782698611 96-97 20.723563175985756 26.831437994414625 28.742965514363743 23.70203331523587 98-99 21.714335680511798 26.71612446448507 27.92944561261067 23.640094242392458 100-101 20.885573184521967 26.87751235361547 28.390697127300328 23.84621733456224 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 498.0 1 400.5 2 340.5 3 432.0 4 488.5 5 516.5 6 649.5 7 1002.0 8 1514.5 9 1944.5 10 2141.5 11 2333.0 12 2549.0 13 2713.0 14 3045.0 15 3446.0 16 3716.5 17 4057.5 18 4560.0 19 5108.0 20 5972.5 21 7153.0 22 8582.5 23 10539.5 24 13151.0 25 16622.5 26 21387.0 27 27075.0 28 32822.5 29 39469.0 30 46720.5 31 54919.5 32 63807.5 33 72077.0 34 80454.0 35 89178.5 36 97670.5 37 105383.5 38 112249.5 39 118622.5 40 125602.5 41 132293.0 42 137925.0 43 143536.0 44 148778.5 45 153869.0 46 159548.0 47 164787.5 48 168547.0 49 168723.0 50 165998.0 51 162286.0 52 157661.5 53 152423.5 54 145068.0 55 134594.5 56 122202.0 57 108940.0 58 95841.5 59 82185.5 60 67197.0 61 52948.5 62 40856.5 63 30473.0 64 21648.0 65 15071.5 66 10396.5 67 7213.0 68 4984.5 69 3271.5 70 2082.5 71 1267.0 72 747.0 73 452.0 74 271.5 75 157.0 76 88.5 77 43.5 78 19.0 79 10.5 80 5.0 81 3.5 82 3.5 83 2.0 84 2.5 85 2.0 86 1.5 87 2.0 88 2.0 89 1.5 90 1.0 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.024668479699646216 2 0.01302014391952059 3 2.406680946306948E-4 4 0.0 5 0.059324685326466266 6 0.0 7 0.0 8 9.626723785227793E-5 9 0.0 10-11 0.0 12-13 0.0 14-15 6.137036413082717E-4 16-17 1.2033404731534741E-5 18-19 3.6100214194604224E-4 20-21 0.0 22-23 0.0 24-25 1.203340473153474E-4 26-27 1.203340473153474E-4 28-29 9.867391879858488E-4 30-31 0.005041996582513057 32-33 0.00558349979543212 34-35 0.0018050107097302111 36-37 0.08187528579336237 38-39 0.07522081297682366 40-41 0.08051551105869895 42-43 0.08428196673966933 44-45 0.08656831363866092 46-47 0.0823686553873553 48-49 0.07275396500685904 50-51 0.06694183052152776 52-53 0.05690597097542778 54-55 0.047182979952347714 56-57 0.06676132945055474 58-59 0.7114509879425285 60-61 0.33700753291136193 62-63 0.003116651825467498 64-65 0.0026112488267430387 66-67 1.1024403744795552 68-69 2.374587855887945 70-71 2.3512550841134994 72-73 1.8085364973165505 74-75 1.283025679285697 76-77 0.7410170633679093 78-79 0.5657866236673004 80-81 0.03802555895164978 82-83 1.9253447570455586E-4 84-85 0.0012394406873480783 86-87 0.0013718081393949604 88-89 2.4066809463069483E-5 90-91 0.3331448099925393 92-93 0.05318764891338355 94-95 0.9477148564414815 96-97 1.6579986041250512 98-99 0.640104931289259 100-101 0.5659550913335418 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 4155100.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.99971537723535 #Duplication Level Percentage of deduplicated Percentage of total 1 70.26495272113542 34.429626843305336 2 15.102441214789533 14.800306420522313 3 5.714255569920232 8.399906895566197 4 2.867959144737727 5.6211672722275186 5 1.641773132429864 4.022320810152773 6 1.0086938409371125 2.9655426665213285 7 0.6668389811869277 2.287244419642354 8 0.48757317142306167 1.9112757300244798 9 0.37027244903434464 1.6328920153243294 >10 1.7275474274878473 14.869542059256874 >50 0.0981951992865133 3.3294852534850303 >100 0.04698996028252638 4.159660342837079 >500 0.0020155820394553705 0.6930027085912486 >1k 3.4412371652058646E-4 0.24001801561534894 >5k 9.832106186302226E-5 0.36007520046348535 >10k+ 4.916053093151113E-5 0.27793334646425566 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11538 0.2776828475848957 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8651 0.2082019686650141 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6297 0.1515486991889485 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 4.813361892613896E-5 2 0.0 0.0 0.0 0.0 4.813361892613896E-5 3 0.0 0.0 0.0 0.0 4.813361892613896E-5 4 0.0 0.0 0.0 0.0 4.813361892613896E-5 5 4.813361892613896E-5 0.0 0.0 0.0 4.813361892613896E-5 6 4.813361892613896E-5 0.0 0.0 0.0 4.813361892613896E-5 7 4.813361892613896E-5 0.0 0.0 0.0 7.220042838920844E-5 8 4.813361892613896E-5 0.0 0.0 0.0 7.220042838920844E-5 9 4.813361892613896E-5 2.406680946306948E-5 0.0 4.813361892613896E-5 7.220042838920844E-5 10-11 4.813361892613896E-5 2.406680946306948E-5 0.0 1.3236745204688215E-4 7.220042838920844E-5 12-13 4.813361892613896E-5 2.406680946306948E-5 0.0 2.2863468989916006E-4 3.489687372145075E-4 14-15 4.813361892613896E-5 2.406680946306948E-5 0.0 2.7676830882529904E-4 4.813361892613896E-4 16-17 4.813361892613896E-5 2.406680946306948E-5 0.0 3.73035546677577E-4 5.776034271136676E-4 18-19 4.813361892613896E-5 2.406680946306948E-5 0.0 4.211691656037159E-4 6.01670236576737E-4 20-21 4.813361892613896E-5 2.406680946306948E-5 0.0 5.655700223821329E-4 6.738706649659455E-4 22-23 4.813361892613896E-5 2.406680946306948E-5 0.0 9.506389737912444E-4 7.46071093355154E-4 24-25 7.220042838920844E-5 2.406680946306948E-5 0.0 0.0013477413299318908 7.942047122812928E-4 26-27 7.220042838920844E-5 2.406680946306948E-5 0.0 0.0017568770908040722 7.942047122812928E-4 28-29 7.220042838920844E-5 2.406680946306948E-5 0.0 0.003104618420735963 8.182715217443623E-4 30-31 7.220042838920844E-5 2.406680946306948E-5 0.0 0.007677312218719165 8.303049264758971E-4 32-33 7.220042838920844E-5 2.406680946306948E-5 0.0 0.01731606940867849 8.423383312074318E-4 34-35 7.220042838920844E-5 2.406680946306948E-5 0.0 0.03084161632692354 8.904719501335708E-4 36-37 7.220042838920844E-5 2.406680946306948E-5 0.0 0.05270631272412216 8.904719501335708E-4 38-39 7.220042838920844E-5 2.406680946306948E-5 0.0 0.08523260571346057 8.904719501335708E-4 40-41 7.220042838920844E-5 2.406680946306948E-5 0.0 0.12274072826165436 8.904719501335708E-4 42-43 7.220042838920844E-5 3.6100214194604216E-5 0.0 0.16144015787827007 8.904719501335708E-4 44-45 7.220042838920844E-5 4.813361892613896E-5 0.0 0.20727539650068588 9.386055690597096E-4 46-47 8.423383312074317E-5 4.813361892613896E-5 0.0 0.2625809246468196 0.0010108059974489182 48-49 9.626723785227792E-5 4.813361892613896E-5 0.0 0.32059396885754854 0.0010589396163750572 50-51 9.626723785227792E-5 4.813361892613896E-5 0.0 0.38216890086881183 0.0010589396163750572 52-53 9.626723785227792E-5 4.813361892613896E-5 0.0 0.4411807176722582 0.0011070732353011961 54-55 1.0830064258381266E-4 4.813361892613896E-5 0.0 0.5105773627590191 0.0011552068542273351 56-57 1.203340473153474E-4 6.01670236576737E-5 0.0 0.5776756275420567 0.001203340473153474 58-59 1.203340473153474E-4 7.220042838920844E-5 0.0 0.6282399942239657 0.0012274072826165435 60-61 1.203340473153474E-4 7.220042838920844E-5 1.203340473153474E-5 0.6710789150682295 0.0012394406873480783 62-63 1.6846766624148638E-4 7.220042838920844E-5 2.406680946306948E-5 0.7096580106375299 0.0012755409015426824 64-65 1.9253447570455584E-4 7.220042838920844E-5 2.406680946306948E-5 0.7548915790233689 0.001299607711005752 66-67 1.9253447570455584E-4 7.220042838920844E-5 2.406680946306948E-5 0.794589781232702 0.0013236745204688214 68-69 1.9253447570455584E-4 7.220042838920844E-5 2.406680946306948E-5 0.8413997256383721 0.001347741329931891 70-71 2.0456788043609058E-4 7.220042838920844E-5 2.406680946306948E-5 0.9164159707347597 0.001347741329931891 72-73 2.1660128516762532E-4 8.423383312074317E-5 2.406680946306948E-5 1.0169310004572694 0.0014680753772472383 74-75 2.1660128516762532E-4 9.626723785227792E-5 3.6100214194604216E-5 1.151067362999687 0.0015763760198310509 76-77 2.1660128516762532E-4 9.626723785227792E-5 4.813361892613896E-5 1.3268513393179466 0.0016124762340256552 78-79 2.1660128516762532E-4 9.626723785227792E-5 4.813361892613896E-5 1.5461601405501673 0.0016365430434887246 80-81 2.1660128516762532E-4 9.626723785227792E-5 4.813361892613896E-5 1.8056484801809822 0.0016846766624148638 82-83 2.1660128516762532E-4 9.626723785227792E-5 4.813361892613896E-5 2.074619142740247 0.0019253447570455585 84-85 2.1660128516762532E-4 9.626723785227792E-5 4.813361892613896E-5 2.315539938870304 0.0019253447570455585 86-87 2.1660128516762532E-4 9.626723785227792E-5 4.813361892613896E-5 2.5423094510360764 0.0019855117807032322 88-89 2.1660128516762532E-4 9.626723785227792E-5 4.813361892613896E-5 2.7623041563379944 0.002045678804360906 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 8745 0.0 35.670708 1 GTATCAA 13685 0.0 33.277645 1 TCAACGC 16940 0.0 26.594872 4 ATCAACG 16980 0.0 26.504757 3 CAACGCA 17235 0.0 26.221779 5 TATCAAC 17280 0.0 26.182999 2 AACGCAG 17775 0.0 25.46261 6 TACATGG 14090 0.0 23.8404 2 GTACATG 14595 0.0 23.70118 1 ACATGGG 14430 0.0 22.296217 3 ACGCAGA 20050 0.0 22.22075 7 CGCAGAG 20120 0.0 21.862257 8 GAGTACT 10620 0.0 19.622282 12-13 AGAGTAC 17800 0.0 19.480907 10-11 GCAGAGT 22140 0.0 18.994759 9 CATGGGG 7695 0.0 18.820784 4 AGTACTT 11545 0.0 18.805613 12-13 GTACTTT 11445 0.0 18.104847 14-15 CATGGGA 7455 0.0 17.46503 4 CAGAGTA 20935 0.0 16.237114 10-11 >>END_MODULE