##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939651_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1697159 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.510696404992107 31.0 31.0 34.0 30.0 34.0 2 31.67821400352 31.0 31.0 34.0 30.0 34.0 3 31.716557494023835 31.0 31.0 34.0 30.0 34.0 4 35.46464356020856 37.0 35.0 37.0 33.0 37.0 5 35.233618653290584 37.0 35.0 37.0 33.0 37.0 6 35.29622681198403 37.0 35.0 37.0 32.0 37.0 7 35.24048954753208 37.0 35.0 37.0 32.0 37.0 8 35.22995959718565 37.0 35.0 37.0 32.0 37.0 9 36.777174089169016 39.0 35.0 39.0 32.0 39.0 10-11 36.66301684167482 39.0 35.0 39.0 32.0 39.0 12-13 36.61382757891276 39.0 35.0 39.0 32.0 39.0 14-15 37.46039292723899 39.0 36.0 41.0 32.0 41.0 16-17 37.46615314180934 39.0 36.0 41.0 32.0 41.0 18-19 37.59503057757111 39.0 36.0 41.0 32.0 41.0 20-21 37.552775844808885 39.0 36.0 41.0 32.0 41.0 22-23 37.417838576114555 39.0 36.0 41.0 32.0 41.0 24-25 37.26285545432101 39.0 36.0 41.0 31.5 41.0 26-27 36.85398627942344 39.0 35.5 40.0 30.5 41.0 28-29 36.62902356231797 38.5 35.0 40.0 30.0 41.0 30-31 36.42283840229466 38.0 35.0 40.0 30.0 41.0 32-33 36.21830158517852 38.0 35.0 40.0 30.0 41.0 34-35 36.17118991208248 38.0 35.0 40.0 30.0 41.0 36-37 35.94967206961752 38.0 35.0 40.0 30.0 41.0 38-39 35.85249201754226 38.0 35.0 40.0 29.5 41.0 40-41 35.70277593319188 38.0 35.0 40.0 29.0 41.0 42-43 35.55285244340689 38.0 35.0 40.0 28.5 41.0 44-45 35.31218760292937 38.0 34.0 40.0 27.0 41.0 46-47 34.88002243749702 38.0 34.0 40.0 25.5 41.0 48-49 34.4997999598152 38.0 33.0 40.0 24.0 41.0 50-51 34.21440183270984 38.0 33.0 40.0 23.5 41.0 52-53 33.83969710557467 38.0 33.0 40.0 23.0 41.0 54-55 33.601935057351724 37.0 33.0 40.0 22.0 41.0 56-57 31.728694541878518 36.0 30.0 39.5 13.5 41.0 58-59 26.860676283129628 32.5 16.5 38.0 2.0 40.0 60-61 27.525680269202827 32.0 18.5 38.0 2.0 40.0 62-63 30.49461394011993 34.5 29.0 38.0 2.0 40.0 64-65 30.61283032408867 35.0 29.5 38.5 2.0 40.0 66-67 24.109783467547828 26.5 16.5 35.5 2.0 38.5 68-69 16.947676086919376 13.0 7.0 28.0 2.0 35.0 70-71 15.842437862333465 12.5 7.0 26.5 2.0 33.5 72-73 16.928493146487746 13.0 7.0 28.5 2.0 34.0 74-75 17.878492233196773 17.0 7.5 29.0 2.0 34.5 76-77 18.918111679577457 21.0 7.0 30.0 2.0 34.0 78-79 21.83859320193335 27.0 9.0 33.0 2.0 35.5 80-81 25.365713819388755 31.0 20.5 34.5 2.0 36.0 82-83 26.627435319849234 33.0 25.5 35.0 2.0 36.0 84-85 26.67745832888963 33.0 25.5 35.0 2.0 36.0 86-87 26.434753314215108 33.0 25.0 35.0 2.0 35.5 88-89 25.92142722043132 33.0 23.0 35.0 2.0 35.0 90-91 24.57219241096444 32.5 11.0 34.5 2.0 35.0 92-93 22.3297613835828 31.5 2.0 34.0 2.0 35.0 94-95 15.40863820066358 4.5 2.0 32.0 2.0 34.5 96-97 12.920749617448925 2.0 2.0 30.0 2.0 34.0 98-99 12.616016531155891 2.0 2.0 29.5 2.0 34.0 100-101 12.330436335075262 2.0 2.0 30.0 2.0 34.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.42361194915005385 1101 2 0.4049559183184712 1101 3 0.3633855199400209 1101 4 0.33488150270784445 1101 5 0.27201376912392305 1101 6 0.30346573449815395 1101 7 0.2653642631475748 1101 8 0.23133473090489076 1101 9 0.5034156524608662 1101 10-11 0.5569749083290034 1101 12-13 0.5455311876019877 1101 14-15 0.5216776770348659 1101 16-17 0.6432244413131087 1101 18-19 0.45293916023091185 1101 20-21 0.35774979579645816 1101 22-23 0.4831356658507602 1101 24-25 0.5553694109523093 1101 26-27 0.3834834153294935 1101 28-29 0.4760422040190093 1101 30-31 0.5436936144250026 1101 32-33 0.41935050883513014 1101 34-35 0.6042636429206354 1101 36-37 0.42742265877237173 1101 38-39 0.8293445952496725 1101 40-41 0.6959541515391194 1101 42-43 0.9027792511063026 1101 44-45 0.7387266459267963 1101 46-47 0.7114035144252213 1101 48-49 0.5999390533413944 1101 50-51 0.6422746571778077 1101 52-53 0.730094461466642 1101 54-55 0.5699242364917225 1101 56-57 -1.64958469950815 1101 58-59 -9.450127765415456 1101 60-61 -10.24597602798983 1101 62-63 -3.6510657604371524 1101 64-65 -4.45661253871938 1101 66-67 -9.423665468715573 1101 68-69 -4.584315075198269 1101 70-71 -4.34472029343004 1101 72-73 -4.525635148819546 1101 74-75 -6.440789132845545 1101 76-77 -7.753767604277396 1101 78-79 -10.840943462057087 1101 80-81 -7.6779973032966815 1101 82-83 -3.947914112489304 1101 84-85 -2.973515639683928 1101 86-87 -2.9256816883032783 1101 88-89 -2.968155386912457 1101 90-91 -9.822349611529074 1101 92-93 -16.517470802707376 1101 94-95 -10.882786169508588 1101 96-97 -9.007767373381641 1101 98-99 -9.064109402803895 1101 100-101 -8.89921394993534 1102 1 0.28991238164369904 1102 2 0.3174073638936292 1102 3 0.42692921418955976 1102 4 0.3002949187880688 1102 5 0.3364945616216275 1102 6 0.30426256918851635 1102 7 0.41813537195250916 1102 8 0.5497898009592248 1102 9 0.4269659898678668 1102 10-11 0.5174012234934722 1102 12-13 0.4306204829970426 1102 14-15 0.5497613670506851 1102 16-17 0.5283546110578357 1102 18-19 0.5533528086495494 1102 20-21 0.5007291314462918 1102 22-23 0.6297222704228389 1102 24-25 0.5160444142652807 1102 26-27 0.4420589399410204 1102 28-29 0.41466915286498107 1102 30-31 0.48126299325535626 1102 32-33 0.585404490513092 1102 34-35 0.43758665010246744 1102 36-37 0.12208094060402885 1102 38-39 0.32187964823143034 1102 40-41 0.32302002722258294 1102 42-43 0.2704134781680523 1102 44-45 0.49072561675369997 1102 46-47 0.38320787171694093 1102 48-49 0.4212437109520195 1102 50-51 0.5121711719295803 1102 52-53 0.2882969538554008 1102 54-55 0.23006262413201028 1102 56-57 -2.3019556699426538 1102 58-59 -9.940625554758526 1102 60-61 -10.40069533737682 1102 62-63 -4.05365702210355 1102 64-65 -5.042946049318314 1102 66-67 -6.077361628591934 1102 68-69 -4.101392378489386 1102 70-71 -4.229351365852503 1102 72-73 -4.323967131148059 1102 74-75 -4.864234917968659 1102 76-77 -7.043613211102292 1102 78-79 -9.548377774825891 1102 80-81 -5.415700577891425 1102 82-83 -2.9927786591970715 1102 84-85 -2.4258449613074085 1102 86-87 -2.3951674803793317 1102 88-89 -2.5349290152983563 1102 90-91 -8.32839256143519 1102 92-93 -12.679323331495825 1102 94-95 -10.259473849893546 1102 96-97 -8.78591345056274 1102 98-99 -8.955872834115196 1102 100-101 -8.831555284315057 1103 1 0.3349018669662094 1103 2 0.244262195466316 1103 3 0.31443228485636254 1103 4 0.28663595785806706 1103 5 0.32308720686366854 1103 6 0.19470514603257527 1103 7 0.19413563546434176 1103 8 0.267007481174133 1103 9 0.420385734443947 1103 10-11 0.4663729916141719 1103 12-13 0.49714690104982395 1103 14-15 0.630839074862898 1103 16-17 0.6307692365991926 1103 18-19 0.5906530268008225 1103 20-21 0.4448265260524735 1103 22-23 0.5133827909936315 1103 24-25 0.5792060958358434 1103 26-27 0.6174675320933645 1103 28-29 0.6153528549362832 1103 30-31 0.7176158387663847 1103 32-33 0.7165359523568569 1103 34-35 0.600310759172558 1103 36-37 0.6971157348802635 1103 38-39 0.6514160698122566 1103 40-41 0.6234764900287217 1103 42-43 0.6868792369094763 1103 44-45 0.8328397673784877 1103 46-47 0.8319399393204279 1103 48-49 0.834131820417376 1103 50-51 0.8041500147872398 1103 52-53 0.8671762825034719 1103 54-55 0.7341065782222174 1103 56-57 -1.9308603774782576 1103 58-59 -9.58628786832093 1103 60-61 -9.627636167462885 1103 62-63 -3.322640489514402 1103 64-65 -4.249845098493029 1103 66-67 -5.669866383908904 1103 68-69 -3.48116689332403 1103 70-71 -3.6229035013102813 1103 72-73 -3.916995735277496 1103 74-75 -4.684218440542377 1103 76-77 -5.7501201673779825 1103 78-79 -8.37447306530601 1103 80-81 -4.2882912206023285 1103 82-83 -2.0696612111027477 1103 84-85 -1.5040674946640742 1103 86-87 -1.4093635847697037 1103 88-89 -1.4620453744221216 1103 90-91 -4.133968852672908 1103 92-93 -8.942777217521403 1103 94-95 -8.125173301161915 1103 96-97 -7.924295059342128 1103 98-99 -8.105299914162073 1103 100-101 -8.217537606005056 1104 1 0.3185415776231828 1104 2 0.3178808672383404 1104 3 0.1466318067387462 1104 4 0.14912305719783348 1104 5 0.31643459242302185 1104 6 0.14214804530205072 1104 7 0.19783083827636005 1104 8 0.3001150385691247 1104 9 0.25509848083875397 1104 10-11 0.34607883518994953 1104 12-13 0.40241092935681166 1104 14-15 0.4949876838930862 1104 16-17 0.4674916748872704 1104 18-19 0.49395832472676204 1104 20-21 0.4178553922969144 1104 22-23 0.4513162720824724 1104 24-25 0.36495600763954883 1104 26-27 0.5734555262186518 1104 28-29 0.39033344846364315 1104 30-31 0.47948913135024185 1104 32-33 0.4432765428068066 1104 34-35 0.5106789939085132 1104 36-37 0.5619099111959116 1104 38-39 0.3909223546818552 1104 40-41 0.3645770482709878 1104 42-43 0.4173155233473693 1104 44-45 0.5543401992362078 1104 46-47 0.5979582111596571 1104 48-49 0.41217197874657785 1104 50-51 0.4454539587808597 1104 52-53 0.3821956265071549 1104 54-55 0.41940181470171467 1104 56-57 -2.3322184524613867 1104 58-59 -9.584504872961059 1104 60-61 -9.868765823084715 1104 62-63 -3.513562534511113 1104 64-65 -4.689698349064866 1104 66-67 -5.971103334884912 1104 68-69 -3.6064363728689006 1104 70-71 -3.34813086363118 1104 72-73 -3.6099098822261677 1104 74-75 -4.376538310501749 1104 76-77 -6.157618611407939 1104 78-79 -8.327717521762027 1104 80-81 -3.930389835402668 1104 82-83 -1.7630111611769053 1104 84-85 -1.3898119486542164 1104 86-87 -1.3440172815671154 1104 88-89 -1.306239921145771 1104 90-91 -3.8877154279770885 1104 92-93 -5.843395199833484 1104 94-95 -7.351251227560997 1104 96-97 -7.324464909130605 1104 98-99 -7.816386443193203 1104 100-101 -7.733148267942495 1105 1 0.4366666881062855 1105 2 0.328934691432444 1105 3 0.33684753993386707 1105 4 0.38191835243041083 1105 5 0.2463947366350041 1105 6 0.2091894439270945 1105 7 0.3359847604140498 1105 8 0.31275012870495544 1105 9 0.4647134599584888 1105 10-11 0.607754957038317 1105 12-13 0.4520510510060163 1105 14-15 0.648426118613564 1105 16-17 0.5676499351472017 1105 18-19 0.5272008321761206 1105 20-21 0.5345302292831704 1105 22-23 0.6856517310355557 1105 24-25 0.6682917369316357 1105 26-27 0.7033445602462791 1105 28-29 0.7896164653265814 1105 30-31 0.9342459448788176 1105 32-33 0.9999409672303514 1105 34-35 0.9034929221480468 1105 36-37 0.6305639872163127 1105 38-39 0.7237654688400923 1105 40-41 0.8422271306926419 1105 42-43 0.8419975408582587 1105 44-45 0.8003839433611759 1105 46-47 0.7575713356669667 1105 48-49 0.747820737208869 1105 50-51 0.7752232617038999 1105 52-53 0.9468064803673784 1105 54-55 1.0070885582997136 1105 56-57 1.9811973459626238 1105 58-59 -7.968305217751887 1105 60-61 -9.117314630635757 1105 62-63 -2.8491259139691607 1105 64-65 -4.0938840589252905 1105 66-67 -5.396857853038227 1105 68-69 -3.318531186002497 1105 70-71 -3.2937489491431826 1105 72-73 -1.4633784585755478 1105 74-75 -3.677363462655155 1105 76-77 -5.547080202384699 1105 78-79 -8.244999802794956 1105 80-81 -3.9133728474870573 1105 82-83 -1.4988410033276693 1105 84-85 -0.9585574186380654 1105 86-87 -0.7890911803533989 1105 88-89 -0.8023203398136651 1105 90-91 -3.3145371001424344 1105 92-93 -5.569833685681122 1105 94-95 -7.333268671611017 1105 96-97 -7.44211400242563 1105 98-99 -7.927431445447141 1105 100-101 -7.926802051569041 1106 1 0.14879421857880004 1106 2 0.12848985292261617 1106 3 0.09491447381726204 1106 4 0.03153686294299973 1106 5 0.005111107633034351 1106 6 0.20519674122417797 1106 7 0.2083665800020995 1106 8 0.17429962426494683 1106 9 0.22187700249642006 1106 10-11 0.2583711448863184 1106 12-13 0.3409905269752542 1106 14-15 0.23632438223639696 1106 16-17 0.14221400696241915 1106 18-19 0.18673053445564847 1106 20-21 0.16086309032275636 1106 22-23 0.20324194857506228 1106 24-25 0.233127607548127 1106 26-27 0.08736661320111949 1106 28-29 0.15584338319930424 1106 30-31 0.2909463897173268 1106 32-33 0.3195088782594553 1106 34-35 0.332241617493068 1106 36-37 0.3190578273311928 1106 38-39 0.28465328804701073 1106 40-41 0.17873083874334128 1106 42-43 0.048644125074183364 1106 44-45 0.29331986955259737 1106 46-47 0.15662572492896487 1106 48-49 0.06043600332659338 1106 50-51 0.16998335269685327 1106 52-53 0.050266915733431006 1106 54-55 -0.1186706133550075 1106 56-57 1.1424172192964726 1106 58-59 -8.424646618351332 1106 60-61 -10.196915527807818 1106 62-63 -4.053514617829823 1106 64-65 -5.524039469346423 1106 66-67 -6.378582915888909 1106 68-69 -3.837320011697404 1106 70-71 -4.026356074373314 1106 72-73 -4.626847840147162 1106 74-75 -5.018985524540252 1106 76-77 -6.7750768822421 1106 78-79 -8.841880750486762 1106 80-81 -4.812348498977332 1106 82-83 -2.5554624313238676 1106 84-85 -2.0827097417412013 1106 86-87 -1.934319711254087 1106 88-89 -2.130566548639788 1106 90-91 -4.575499989336262 1106 92-93 -6.807178831407793 1106 94-95 -7.452082573791196 1106 96-97 -7.223415157060234 1106 98-99 -6.640432068173213 1106 100-101 -7.101321232887577 1107 1 0.04370722702980245 1107 2 -0.014293476524933624 1107 3 0.09015573484269623 1107 4 0.08610904580722689 1107 5 0.04380890058460807 1107 6 -0.04316064934483421 1107 7 0.017358361376338394 1107 8 0.10269693453287942 1107 9 0.010291440992922674 1107 10-11 0.018690402447624876 1107 12-13 0.09779161099358902 1107 14-15 0.23532081116233172 1107 16-17 0.15240130175647693 1107 18-19 0.10040784047615148 1107 20-21 0.16897399137069158 1107 22-23 0.14931919180745012 1107 24-25 0.2855458733496121 1107 26-27 0.10115518345571672 1107 28-29 0.14485065384762663 1107 30-31 0.2762485075081429 1107 32-33 0.08395591579151329 1107 34-35 0.10882499757122588 1107 36-37 0.18046883633466848 1107 38-39 0.2000049438142426 1107 40-41 0.129245570481892 1107 42-43 -0.07447867570667199 1107 44-45 -0.019628101721842484 1107 46-47 0.11558588140680826 1107 48-49 -0.012696377258642144 1107 50-51 0.09392893940793101 1107 52-53 0.20017912009642913 1107 54-55 -0.0914001035324361 1107 56-57 0.946344400471741 1107 58-59 -8.809185352693735 1107 60-61 -10.162493251569348 1107 62-63 -3.9413302993155632 1107 64-65 -5.291317935408141 1107 66-67 -6.9352559229240995 1107 68-69 -3.939332424661611 1107 70-71 -3.768666069148667 1107 72-73 -2.252801299995472 1107 74-75 -4.51831282221209 1107 76-77 -6.293058829119969 1107 78-79 -7.3203069540603 1107 80-81 -2.4649994116749205 1107 82-83 -1.4335499945832808 1107 84-85 -1.0679524185770823 1107 86-87 -1.075642024349797 1107 88-89 -0.7944691147192806 1107 90-91 -0.22456636237615157 1107 92-93 -2.8747653131880817 1107 94-95 -5.7031738635560405 1107 96-97 -6.158107822315708 1107 98-99 -5.7505605760552 1107 100-101 -6.407797257599626 1108 1 -0.10422861656657645 1108 2 -0.11660091383512494 1108 3 -0.14111367774821204 1108 4 -0.15963499157177807 1108 5 -0.07499544877840236 1108 6 -0.2719050307891848 1108 7 -0.1570664289244732 1108 8 -0.10710468587349453 1108 9 -0.24350812444783543 1108 10-11 -0.1329635463699006 1108 12-13 -0.1338737124042595 1108 14-15 -0.2514355010227689 1108 16-17 -0.2544707357859224 1108 18-19 -0.1048158255636551 1108 20-21 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88-89 1.5998647319528878 2316 90-91 2.6286281160875475 2316 92-93 4.537556977260344 2316 94-95 11.142976517506543 2316 96-97 12.84318068630829 2316 98-99 12.47034169905875 2316 100-101 11.559367826350512 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 7.0 4 41.0 5 118.0 6 308.0 7 617.0 8 1193.0 9 2036.0 10 3509.0 11 5696.0 12 8058.0 13 11081.0 14 13530.0 15 17874.0 16 24426.0 17 32460.0 18 33408.0 19 31096.0 20 31424.0 21 31282.0 22 31234.0 23 27051.0 24 27121.0 25 31048.0 26 43629.0 27 64051.0 28 89371.0 29 118954.0 30 146401.0 31 159113.0 32 157468.0 33 144067.0 34 128423.0 35 114592.0 36 88917.0 37 55412.0 38 20710.0 39 1433.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.209709692305786 18.89619777487937 14.612984958599458 20.281107574215383 2 14.474668916904243 23.35460216404656 39.34564756989663 22.825081349152565 3 21.46695082882922 28.367919686675275 31.057954114920694 19.10717536957481 4 11.709568755785403 16.869309239735347 39.53059200699522 31.89052999748403 5 11.70655889480737 40.94982507135519 33.74491278941117 13.598703244426268 6 27.07459937460191 38.06685172102319 18.41866318948313 16.43988571489177 7 25.01026715823326 36.54236285462941 21.0866512801688 17.360718706968527 8 24.005149840319596 32.47999575756862 21.081584312431502 22.433270089680285 9 25.993911000678192 18.981368274864053 21.966945937298746 33.05777478715901 10-11 23.513412709121535 26.222204283747132 27.91703664771539 22.347346359415944 12-13 29.05567480713357 24.312512852360914 27.118997100448457 19.512815240057062 14-15 24.55613770867361 25.325870224635683 29.426750053619365 20.69124201307134 16-17 19.63725850082402 28.96570091547109 30.23720818143733 21.159832402267554 18-19 19.22793190490444 29.710704260539394 31.882543502188366 19.178820332367795 20-21 21.37021339780186 27.26765730258626 29.95606186574151 21.40606743387037 22-23 20.194567133645073 27.2238137774342 29.525493297005806 23.056125791914926 24-25 21.526722909711413 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22.07644355097495 50-51 22.24910391358167 28.17685603364855 29.609473549782233 19.964566502987545 52-53 20.905692891360847 28.55981897111476 30.120290792685477 20.414197344838914 54-55 19.191561815890147 27.539998083571042 31.056055546956195 22.212384553582616 56-57 20.45069165815474 29.106579580383784 28.91790514199314 21.524823619468332 58-59 19.97899600421277 27.54980218593594 30.266494556409558 22.20470725344173 60-61 20.59528093048738 26.917471810704157 30.508880646188246 21.978366612620217 62-63 23.20254698102846 26.61222178486661 29.191169711526 20.994061522578942 64-65 21.65544561464572 27.846221688658947 30.29979013068531 20.19854256601002 66-67 20.262145630455734 27.460790760943006 30.494503349638613 21.78256025896265 68-69 20.749828674700257 28.87935060130632 29.289773244143102 21.081047479850326 70-71 20.704611725856413 28.589004264085492 28.561253625987444 22.14513038407065 72-73 19.876243478976335 27.641366234240795 28.977000138542447 23.50539014824042 74-75 20.45176290939041 28.6635134343029 28.923677648872996 21.961046007433694 76-77 19.483996425157642 27.556435089749044 30.38284338610837 22.576725098984944 78-79 19.362276275376075 27.450502822802246 30.86245946608236 22.32476143573932 80-81 20.081230788821173 29.63349757527952 28.995571480618153 21.289700155281153 82-83 20.138850016645506 29.0038034239654 28.26556796521237 22.591778594176727 84-85 20.375952143510546 27.768577754274425 28.129740463097857 23.725729639117173 86-87 21.097072891210424 28.007029421339567 28.66265700931413 22.233240678135875 88-89 22.015526576985184 26.704640031299448 28.75572023643104 22.524113155284333 90-91 19.911249987078826 27.34743784176508 29.980463183511695 22.7608489876444 92-93 18.46218277624834 29.258881555208262 30.098028660901992 22.18090700764141 94-95 18.29070310938376 29.541024357766243 28.71859844027335 23.449674092576643 96-97 17.998579392374783 28.81792827350289 27.987751259030862 25.195741075091462 98-99 18.724883893331565 28.53742499716886 28.10912338606278 24.628567723436802 100-101 20.014822335689622 28.477918020944582 28.181292868118984 23.32596677524682 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 4266.0 1 3110.0 2 1891.5 3 1833.0 4 1833.0 5 2068.5 6 2808.5 7 3929.0 8 4835.0 9 5311.0 10 5395.5 11 5355.5 12 5520.5 13 5664.5 14 5800.0 15 5789.5 16 5783.0 17 5851.5 18 5812.0 19 5951.0 20 6166.0 21 6488.5 22 7068.5 23 7791.0 24 8712.5 25 10015.0 26 11634.0 27 13388.0 28 15506.5 29 18029.0 30 20651.5 31 23338.0 32 26116.5 33 28951.5 34 31741.0 35 34591.5 36 37552.5 37 40168.0 38 41994.5 39 43264.0 40 44687.5 41 46596.5 42 48940.5 43 52918.5 44 59538.0 45 68954.5 46 81838.5 47 97475.0 48 112651.0 49 104556.0 50 79216.0 51 61429.0 52 49183.5 53 41309.5 54 36113.0 55 31855.5 56 28164.0 57 25044.5 58 21970.5 59 19071.0 60 16163.0 61 13161.0 62 10449.0 63 7825.5 64 5527.5 65 3965.0 66 2745.0 67 1830.0 68 1232.5 69 827.5 70 585.0 71 431.0 72 350.5 73 283.5 74 179.5 75 110.5 76 65.5 77 35.0 78 18.0 79 9.0 80 4.0 81 2.0 82 0.5 83 1.0 84 1.0 85 0.0 86 0.5 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.026043523323389266 2 0.009015065765788591 3 3.535319908152389E-4 4 0.0 5 0.1454194922220016 6 0.0 7 0.0 8 0.001119517970914923 9 0.0 10-11 0.0 12-13 0.0 14-15 6.481419831612713E-4 16-17 0.0 18-19 1.7676599540761946E-4 20-21 0.0 22-23 4.1245398928444534E-4 24-25 5.892199846920647E-5 26-27 4.1245398928444534E-4 28-29 0.0040066958959060405 30-31 0.013610981646386699 32-33 0.0032996319142755627 34-35 0.004978908870647948 36-37 0.10950653415502025 38-39 0.10894677516956278 40-41 0.12052494786876185 42-43 0.1218801538335536 44-45 0.11802076293382059 46-47 0.11159826510067708 48-49 0.11165718709914628 50-51 0.11021359813665071 52-53 0.08944359367625543 54-55 0.0763923710153262 56-57 0.5498011677161656 58-59 1.5628765483964673 60-61 0.5183073595343748 62-63 0.00536190186069779 64-65 0.12117308985192313 66-67 1.8886857389319445 68-69 2.8429275041407434 70-71 2.7543972014407605 72-73 2.606473524283818 74-75 1.9907681012798446 76-77 1.4022551805694106 78-79 0.9339725977353919 80-81 0.05191028065137091 82-83 2.3568799387682589E-4 84-85 8.838299770380973E-5 86-87 3.8299299004984215E-4 88-89 2.651489931114292E-4 90-91 0.24756077656837103 92-93 0.3908296158462466 94-95 1.9872327813716923 96-97 2.405520048504589 98-99 1.4026676345586948 100-101 0.9375963006412481 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1697159.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 40.10937624604394 #Duplication Level Percentage of deduplicated Percentage of total 1 70.32127296789285 28.205423955699732 2 14.50548694881389 11.636120673241122 3 6.01551139805244 7.238352299305532 4 3.0430475427956427 4.882189553143596 5 1.724953481289308 3.459340409397808 6 1.1145403363288338 2.6822110614723322 7 0.7110694015884843 1.9964385115753283 8 0.5021832125211981 1.6113804332367823 9 0.35675240373444206 1.2878204749258775 >10 1.554961856996097 10.658342479692081 >50 0.08464977525803055 2.337243495636865 >100 0.05186722715447367 3.9647903009276284 >500 0.004567815858112931 1.2082552045931487 >1k 0.0060413048446009724 5.463448242917455 >5k 0.0017681867837856505 4.80039721863989 >10k+ 0.0013261400878392378 8.56824568559484 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 29371 1.7305980170390634 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 24631 1.4513077442950248 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16131 0.9504707573067697 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 15922 0.9381560596267056 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 13918 0.8200763746944157 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11889 0.7005236398003959 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 11411 0.6723589245321152 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 11281 0.6646990647311183 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 10423 0.6141439900445391 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9812 0.5781426489798539 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 9103 0.5363669520651866 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 7711 0.4543475301960511 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 7708 0.45417076420064356 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 7052 0.4155179332048441 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6497 0.3828162240544345 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 6319 0.37232810832691576 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 5956 0.35093942288259383 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 5555 0.32731170149644195 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 5374 0.3166468197735156 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 5077 0.29914698622816127 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 5060 0.2981453122541848 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 4800 0.2828255926521911 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 4101 0.24163911572221575 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 3785 0.22301976420594652 No Hit GCTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 3751 0.22101641625799348 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 3746 0.22072180626564747 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 3652 0.21518313840954206 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 3513 0.20699298062232235 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 3427 0.2019256887539706 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 2897 0.17069702956529118 No Hit GATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTT 2869 0.1690472136081534 No Hit CCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 2827 0.16657248967244673 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2752 0.1621533397872562 No Hit GTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGC 2689 0.15844125388369623 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 2676 0.15767526790359654 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 2633 0.15514162196942066 No Hit TGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGT 2387 0.14064681034599585 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2159 0.12721259469501678 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 2143 0.12626984271950947 No Hit AAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTAC 2073 0.12214530282666503 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2042 0.12031872087411963 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 1926 0.11348376905169168 No Hit ATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGT 1889 0.11130365510833104 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 1854 0.10924138516190882 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 1820 0.10723803721395578 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 1814 0.10688450522314055 No Hit GCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAAC 1700 0.10016739739765101 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 3.8299299004984215E-4 0.0 12-13 0.0 0.0 0.0 7.659859800996842E-4 0.0 14-15 0.0 0.0 0.0 8.543689778034939E-4 0.0 16-17 0.0 0.0 0.0 0.0012668229670879394 0.0 18-19 0.0 0.0 0.0 0.001738198954841591 0.0 20-21 0.0 0.0 0.0 0.0028282559265219113 0.0 22-23 0.0 0.0 0.0 0.005980582844624458 0.0 24-25 0.0 0.0 0.0 0.009486441753542244 0.0 26-27 0.0 0.0 0.0 0.01263876867164479 0.0 28-29 0.0 0.0 0.0 0.01973886948718417 0.0 30-31 0.0 0.0 0.0 0.03788684501569976 0.0 32-33 0.0 0.0 0.0 0.0726508241125316 0.0 34-35 0.0 0.0 0.0 0.13110144659398443 0.0 36-37 0.0 0.0 0.0 0.21671511036974145 0.0 38-39 0.0 0.0 0.0 0.3157040677980083 0.0 40-41 0.0 0.0 0.0 0.42868699986271175 0.0 42-43 0.0 0.0 0.0 0.5431429818891453 0.0 44-45 0.0 0.0 0.0 0.6682638456385053 0.0 46-47 0.0 0.0 0.0 0.8265283335267939 0.0 48-49 0.0 0.0 0.0 0.9790184655651002 0.0 50-51 0.0 0.0 0.0 1.1176913889623776 0.0 52-53 0.0 0.0 0.0 1.2351523929107409 0.0 54-55 0.0 0.0 0.0 1.3550881207948107 0.0 56-57 0.0 0.0 0.0 1.4605879590539248 0.0 58-59 0.0 0.0 0.0 1.5401326569873535 0.0 60-61 0.0 0.0 0.0 1.6187935249437442 0.0 62-63 0.0 0.0 0.0 1.6818106023065607 0.0 64-65 0.0 0.0 0.0 1.729301733072741 0.0 66-67 0.0 0.0 5.892199846920648E-5 1.7721380259598543 0.0 68-69 0.0 0.0 5.892199846920648E-5 1.829999428456615 0.0 70-71 0.0 2.946099923460324E-5 5.892199846920648E-5 1.9254236049774947 0.0 72-73 0.0 5.892199846920648E-5 5.892199846920648E-5 2.0737891971229567 0.0 74-75 0.0 5.892199846920648E-5 5.892199846920648E-5 2.261691450241256 0.0 76-77 0.0 5.892199846920648E-5 5.892199846920648E-5 2.5145257456726213 0.0 78-79 0.0 5.892199846920648E-5 5.892199846920648E-5 2.9110707953703807 0.0 80-81 0.0 5.892199846920648E-5 5.892199846920648E-5 3.4045130715507503 0.0 82-83 0.0 5.892199846920648E-5 5.892199846920648E-5 3.873856250357215 0.0 84-85 0.0 5.892199846920648E-5 5.892199846920648E-5 4.317538898830339 0.0 86-87 2.946099923460324E-5 5.892199846920648E-5 5.892199846920648E-5 4.663705639836927 0.0 88-89 5.892199846920648E-5 5.892199846920648E-5 5.892199846920648E-5 4.932360491857274 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 12295 0.0 24.701744 1 TACATGG 12295 0.0 24.274742 2 ACATGGG 12300 0.0 23.1536 3 AGTACTT 10200 0.0 21.728132 12-13 CATGGGG 2405 0.0 21.53009 4 ATGGGAG 3015 0.0 21.233416 5 CATGGGA 9670 0.0 21.175373 4 CGGCTTG 505 0.0 21.120993 74-75 GAGTACT 10200 0.0 21.036888 12-13 GCTTACG 480 0.0 20.999144 60-61 CTTTGCG 680 0.0 20.768555 2 GTAGCAA 1215 0.0 20.213724 64-65 GGTATCG 415 0.0 19.894064 62-63 GTCGGGA 500 0.0 19.771662 2 GCTTATA 2765 0.0 19.754192 1 ATGGGAT 3075 0.0 19.594452 5 GTACTTT 11165 0.0 19.260752 14-15 TACGCGG 180 6.91216E-11 18.91284 64-65 CGTTTAG 440 0.0 18.763718 62-63 ATGCGCG 80 0.0010519444 18.146666 92-93 >>END_MODULE