FastQCFastQC Report
Fri 27 May 2016
SRR939650_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939650_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1679218
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA120090.7151543158779861No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA92930.5534123621828732No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63570.37856907203233886No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT51030.30389145423643626No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT43740.26047838934551676No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43040.256309782291519No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA41580.2476152590074666No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT38740.23070262467410427No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG38450.22897563032316234No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34420.2049763639980038No Hit
TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC32560.19389977954023838No Hit
GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG31240.18603897766698546No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT28620.1704364769791653No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA27040.16102733534299896No Hit
TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG24420.14542483465517878No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT23080.1374449297232402No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22620.13470555937347026No Hit
GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT21150.125951484560075No Hit
ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG20420.12160422291804876No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC18960.1129096996339963No Hit
ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA17700.10540620693680035No Hit
CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG17210.1024881819990019No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG2803.42352E-728.831021
GTACATG111500.028.6888071
GTCTAAG3457.204471E-826.3239771
TACATGG130450.024.3727252
GAGTACT93500.023.67513812-13
ACATGGG132300.023.6034073
GCTTATA23600.023.0892491
ATGGGAG30200.022.9749535
GTACGTG2652.2236416E-422.8472271
ATGGGAT31350.022.132175
CATGGGG26450.021.9684564
AGTACTT98200.021.9412112-13
CGTCACT2300.002623190621.9366471
GTACTTT102800.021.6280514-15
CATGGGA102000.021.3974444
GTATAGC3455.5922676E-520.4742031
GTATTAT8400.020.4219761
GTCCTAT4109.439253E-619.6894781
TACCTGG9650.019.5823922
GTATTAC3157.1412337E-419.2206821
Produced by FastQC (version 0.11.5)