FastQCFastQC Report
Fri 27 May 2016
SRR939649_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939649_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2074
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCTATAGGATCATTTATTTCACTAACAGCTGTTCTCATCATGATCTTT140.6750241080038573No Hit
ACTCAGACTACCCAGATGCTTACACCACATGAAACACTGTCTCTTCTATA120.5785920925747349No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110.5303760848601736No Hit
TGCTTACACCACATGAAACACTGTCTCTTCTATAGGATCATTTATTTCAC100.4821600771456124No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT100.4821600771456124No Hit
ACATGAAACACTGTCTCTTCTATAGGATCATTTATTTCACTAACAGCTGT90.43394406943105107No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80.3857280617164899No Hit
ACGTAACACAGTTCCACCCGCCTCACATTGAAATCCAAATGCTGAAGAAC80.3857280617164899No Hit
ACCCAGATGCTTACACCACATGAAACACTGTCTCTTCTATAGGATCATTT80.3857280617164899No Hit
ATACTGAACTGCTACGTAACACAGTTCCACCCGCCTCACATTGAAATCCA80.3857280617164899No Hit
CTATAGGATCATTTATTTCACTAACAGCTGTTCTCATCATGATCTTTATA60.28929604628736744No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60.28929604628736744No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA60.28929604628736744No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT60.28929604628736744No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA60.28929604628736744No Hit
TCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCTTG60.28929604628736744RNA PCR Primer, Index 26 (100% over 29bp)
TCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCTTG60.28929604628736744RNA PCR Primer, Index 31 (96% over 29bp)
TCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCTTG60.28929604628736744TruSeq Adapter, Index 1 (96% over 28bp)
ACACAGTTCCACCCGCCTCACATTGAAATCCAAATGCTGAAGAACGGGAA60.28929604628736744No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT50.2410800385728062No Hit
CATGAAACACTGTCTCTTCTATAGGATCATTTATTTCACTAACAGCTGTT50.2410800385728062No Hit
GGAGTAAACATAACATTCTTCCCTCAACATTTCCTGGGCCTTTCAGGAAT40.19286403085824494No Hit
TCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCTTG40.19286403085824494RNA PCR Primer, Index 33 (96% over 29bp)
CAGCTGTTCTCATCATGATCTTTATAATTTGAGAGGCCTTTGCTTCAAAA40.19286403085824494No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA40.19286403085824494No Hit
CCCTAGATGACACATGAGCAAAAGCCCACTTCGCCATCATATTCGTAGGA40.19286403085824494No Hit
CATCATGATCTTTATAATTTGAGAGGCCTTTGCTTCAAAACGAGAAGTAA40.19286403085824494No Hit
CTCTTCTATAGGATCATTTATTTCACTAACAGCTGTTCTCATCATGATCT40.19286403085824494No Hit
GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT30.14464802314368372No Hit
GTACATGGGGGGCACGCCCCTCTGGCGAGCCTTCAGTATTTTGGGTTGGC30.14464802314368372No Hit
CTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCTTGA30.14464802314368372RNA PCR Primer, Index 26 (100% over 29bp)
TTCCTGGGCCTTTCAGGAATACCACGACGCTACTCAGACTACCCAGATGC30.14464802314368372No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG30.14464802314368372No Hit
TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC30.14464802314368372No Hit
CTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGCTTGA30.14464802314368372RNA PCR Primer, Index 17 (96% over 28bp)
GTTCCACCCGCCTCACATTGAAATCCAAATGCTGAAGAACGGGAAAAAAA30.14464802314368372No Hit
ATGAAACACTGTCTCTTCTATAGGATCATTTATTTCACTAACAGCTGTTC30.14464802314368372No Hit
TCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTTG30.14464802314368372RNA PCR Primer, Index 46 (96% over 28bp)
ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT30.14464802314368372No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30.14464802314368372No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT30.14464802314368372No Hit
CATTGAAATCCAAATGCTGAAGAACGGGAAAAAAATTCCTAAAGTAGAGA30.14464802314368372No Hit
GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG30.14464802314368372No Hit
GTGCTCGCCCACGTCGCTGTCGTAGCGCACGTACTCCTCCCGGTTGTAGA30.14464802314368372No Hit
CTATGTAGTAGCCCATTTCCACTATGTTCTATCAATGGGAGCAGTGTTTG30.14464802314368372No Hit
GCTGTTCTCATCATGATCTTTATAATTTGAGAGGCCTTTGCTTCAAAACG30.14464802314368372No Hit
GTCCTGGACAGGGAGATGACGACATTGGGCTGTTCAAGCCGCCGCAGGGA30.14464802314368372No Hit
TTTCAGGAATACCACGACGCTACTCAGACTACCCAGATGCTTACACCACA30.14464802314368372No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT30.14464802314368372No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC30.14464802314368372No Hit
ACATAACATTCTTCCCTCAACATTTCCTGGGCCTTTCAGGAATACCACGA30.14464802314368372No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers