##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939648_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2864 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 15.042597765363128 2.0 2.0 31.0 2.0 31.0 2 19.51850558659218 16.0 16.0 31.0 2.0 31.0 3 21.32332402234637 25.0 16.0 31.0 2.0 33.0 4 25.254888268156424 32.0 19.0 35.0 2.0 37.0 5 25.54399441340782 33.0 19.0 35.0 2.0 37.0 6 25.724162011173185 33.0 17.0 35.0 2.0 37.0 7 25.68400837988827 33.0 16.0 35.0 2.0 37.0 8 25.50558659217877 33.0 15.0 35.0 2.0 37.0 9 26.245460893854748 33.0 11.0 37.0 2.0 39.0 10-11 25.958275139664806 32.5 11.0 37.0 2.0 39.0 12-13 25.769553072625698 32.5 10.5 37.0 2.0 39.0 14-15 25.969448324022345 32.5 10.0 38.0 2.0 40.0 16-17 25.49266759776536 32.5 10.0 38.0 2.0 40.0 18-19 25.634252793296092 32.5 10.0 38.0 2.0 40.0 20-21 25.46508379888268 32.0 10.0 38.0 2.0 40.0 22-23 25.17353351955307 32.0 8.0 38.0 2.0 40.0 24-25 24.946927374301676 32.0 2.0 38.0 2.0 40.0 26-27 24.829085195530727 32.0 2.0 38.0 2.0 40.0 28-29 24.429469273743017 31.5 2.0 38.0 2.0 40.0 30-31 24.268680167597765 31.5 2.0 38.0 2.0 40.0 32-33 23.95792597765363 31.0 2.0 38.0 2.0 40.0 34-35 23.580831005586592 30.0 2.0 38.0 2.0 40.0 36-37 23.076641061452513 30.0 2.0 38.0 2.0 40.0 38-39 22.810579608938546 30.0 2.0 37.5 2.0 40.0 40-41 22.441340782122907 30.0 2.0 37.5 2.0 40.0 42-43 22.17929469273743 30.0 2.0 37.0 2.0 40.0 44-45 21.67161312849162 28.5 2.0 37.0 2.0 39.5 46-47 21.25768156424581 27.0 2.0 37.0 2.0 39.5 48-49 20.991270949720672 27.0 2.0 36.5 2.0 39.0 50-51 20.57087988826816 26.0 2.0 35.5 2.0 38.5 52-53 20.52758379888268 26.5 2.0 36.0 2.0 39.0 54-55 20.59881284916201 27.0 2.0 36.0 2.0 39.0 56-57 20.19832402234637 26.0 2.0 36.0 2.0 39.0 58-59 19.736557262569832 24.5 2.0 36.0 2.0 39.0 60-61 19.343051675977655 23.5 2.0 35.0 2.0 38.5 62-63 18.835719273743017 22.0 2.0 34.5 2.0 38.0 64-65 18.538058659217874 22.0 2.0 34.0 2.0 38.0 66-67 17.873777932960895 19.0 2.0 34.0 2.0 37.5 68-69 17.443959497206706 16.0 2.0 33.5 2.0 37.0 70-71 17.012046089385475 11.5 2.0 33.0 2.0 36.5 72-73 16.351431564245807 7.5 2.0 32.0 2.0 36.0 74-75 15.998079608938546 4.0 2.0 32.0 2.0 35.5 76-77 15.543470670391061 2.0 2.0 31.5 2.0 35.0 78-79 14.927898044692737 2.0 2.0 31.0 2.0 35.0 80-81 14.439420391061454 2.0 2.0 31.0 2.0 34.0 82-83 13.677723463687151 2.0 2.0 29.5 2.0 34.0 84-85 13.207576815642458 2.0 2.0 29.0 2.0 34.0 86-87 12.569483240223464 2.0 2.0 29.0 2.0 34.0 88-89 11.309357541899441 2.0 2.0 26.0 2.0 33.5 90-91 10.116620111731844 2.0 2.0 22.5 2.0 32.5 92-93 9.119937150837988 2.0 2.0 16.0 2.0 31.5 94-95 8.635474860335195 2.0 2.0 8.5 2.0 31.0 96-97 8.543121508379889 2.0 2.0 4.5 2.0 31.0 98-99 8.129015363128492 2.0 2.0 2.0 2.0 31.0 100-101 7.496508379888269 2.0 2.0 2.0 2.0 30.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 22.499960909403633 1101 2 17.00667917565751 1101 3 14.55621696844608 1101 4 13.316676673859341 1101 5 13.189050880320227 1101 6 13.128548331613345 1101 7 13.342267254589238 1101 8 13.205270037839693 1101 9 14.787513525346338 1101 10-11 14.680800575413578 1101 12-13 14.400596991587701 1101 14-15 16.585258154298405 1101 16-17 16.185230321793792 1101 18-19 15.935059417706473 1101 20-21 17.50900616067799 1101 22-23 18.461707477249274 1101 24-25 14.185137598899214 1101 26-27 15.011144572661596 1101 28-29 17.643040935672516 1101 30-31 18.119810801513584 1101 32-33 19.663408856365518 1101 34-35 19.311472777308694 1101 36-37 19.581368014510428 1101 38-39 19.8712618444507 1101 40-41 20.425633736748285 1101 42-43 21.139902117146697 1101 44-45 21.906071238702815 1101 46-47 22.38193607905683 1101 48-49 21.839711980485973 1101 50-51 19.806443850267385 1101 52-53 20.61417909747631 1101 54-55 19.261830221721866 1101 56-57 19.968948775682524 1101 58-59 18.83123588829471 1101 60-61 20.710523501266536 1101 62-63 19.976901992056792 1101 64-65 19.331385370735216 1101 66-67 19.12700050035964 1101 68-69 20.612100728648713 1101 70-71 19.054485567751826 1101 72-73 21.841951246208218 1101 74-75 23.268763642618133 1101 76-77 18.16394424117334 1101 78-79 19.050356662601246 1101 80-81 15.158510648278448 1101 82-83 13.009683209807047 1101 84-85 19.713677017856586 1101 86-87 22.08211605216249 1101 88-89 18.51027957594521 1101 90-91 18.40927416580667 1101 92-93 15.541769865841072 1101 94-95 21.511258873565374 1101 96-97 16.537410482534323 1101 98-99 21.382384213653562 1101 100-101 8.530830753353973 1102 1 4.999960909403635 1102 2 1.00667917565751 1102 3 -1.4437830315539202 1102 4 -4.183323326140659 1102 5 -4.310949119679773 1102 6 -4.371451668386655 1102 7 -4.157732745410762 1102 8 -4.294729962160307 1102 9 -4.712486474653662 1102 10-11 -4.069199424586422 1102 12-13 -3.349403008412299 1102 14-15 -2.9147418457015952 1102 16-17 -3.0647696782062077 1102 18-19 -3.564940582293527 1102 20-21 -1.9909938393220088 1102 22-23 -1.0382925227507265 1102 24-25 -1.5648624011007861 1102 26-27 -0.9888554273384038 1102 28-29 -0.3569590643274836 1102 30-31 -1.1301891984864163 1102 32-33 0.4134088563655176 1102 34-35 -0.43852722269130595 1102 36-37 0.33136801451042786 1102 38-39 -2.3787381555493 1102 40-41 -0.324366263251715 1102 42-43 -0.11009788285330302 1102 44-45 1.4060712387028147 1102 46-47 2.1319360790568282 1102 48-49 1.0897119804859727 1102 50-51 2.556443850267385 1102 52-53 2.8641790974763097 1102 54-55 2.7618302217218655 1102 56-57 2.9689487756825237 1102 58-59 3.83123588829471 1102 60-61 4.710523501266536 1102 62-63 4.976901992056792 1102 64-65 4.331385370735216 1102 66-67 3.127000500359639 1102 68-69 4.362100728648715 1102 70-71 5.304485567751824 1102 72-73 6.591951246208218 1102 74-75 5.518763642618131 1102 76-77 6.413944241173342 1102 78-79 5.800356662601244 1102 80-81 7.9085106482784475 1102 82-83 9.009683209807047 1102 84-85 7.463677017856586 1102 86-87 9.08211605216249 1102 88-89 9.010279575945209 1102 90-91 7.659274165806673 1102 92-93 9.791769865841072 1102 94-95 8.511258873565374 1102 96-97 8.787410482534323 1102 98-99 8.632384213653562 1102 100-101 9.030830753353973 1103 1 -1.5000390905963652 1103 2 -12.326654157675822 1103 3 -14.777116364887252 1103 4 -16.01665665947399 1103 5 -11.477615786346439 1103 6 -11.538118335053321 1103 7 -10.657732745410762 1103 8 -10.794729962160307 1103 9 -14.545819807986996 1103 10-11 -16.069199424586422 1103 12-13 -13.516069675078965 1103 14-15 -11.331408512368263 1103 16-17 -11.814769678206208 1103 18-19 -11.814940582293527 1103 20-21 -14.990993839322009 1103 22-23 -14.038292522750726 1103 24-25 -11.064862401100786 1103 26-27 -9.15552209400507 1103 28-29 -9.356959064327484 1103 30-31 -11.046855865153084 1103 32-33 -8.586591143634482 1103 34-35 -6.938527222691306 1103 36-37 -10.085298652156238 1103 38-39 -11.6287381555493 1103 40-41 -7.824366263251715 1103 42-43 -14.52676454951997 1103 44-45 -10.260595427963851 1103 46-47 -11.784730587609838 1103 48-49 -6.576954686180693 1103 50-51 -8.943556149732615 1103 52-53 -5.219154235857022 1103 54-55 -4.9881697782781345 1103 56-57 -5.447717890984144 1103 58-59 -3.252097445038622 1103 60-61 -5.456143165400132 1103 62-63 -8.60643134127654 1103 64-65 -14.668614629264784 1103 66-67 -13.622999499640361 1103 68-69 -13.387899271351285 1103 70-71 -12.695514432248176 1103 72-73 -11.908048753791782 1103 74-75 -11.981236357381869 1103 76-77 -12.086055758826658 1103 78-79 -11.699643337398756 1103 80-81 -11.341489351721552 1103 82-83 -9.990316790192953 1103 84-85 -9.786322982143414 1103 86-87 -9.66788394783751 1103 88-89 -8.989720424054791 1103 90-91 -8.590725834193327 1103 92-93 -7.958230134158928 1103 94-95 -7.9887411264346255 1103 96-97 -7.212589517465677 1103 98-99 -6.3676157863464375 1103 100-101 -5.719169246646027 1104 1 4.499960909403635 1104 2 0.50667917565751 1104 3 -1.4437830315539202 1104 4 2.316676673859341 1104 5 10.189050880320227 1104 6 -1.8714516683866549 1104 7 -13.157732745410762 1104 8 -1.7947299621603072 1104 9 -5.212486474653662 1104 10-11 -2.3191994245864223 1104 12-13 3.900596991587701 1104 14-15 -0.9147418457015952 1104 16-17 -4.064769678206208 1104 18-19 2.685059417706473 1104 20-21 -1.9909938393220088 1104 22-23 -1.7882925227507265 1104 24-25 -10.564862401100786 1104 26-27 -11.988855427338404 1104 28-29 -1.8569590643274836 1104 30-31 -1.8801891984864163 1104 32-33 -11.086591143634482 1104 34-35 -8.438527222691306 1104 36-37 -1.4186319854895721 1104 38-39 -11.3787381555493 1104 40-41 -10.824366263251715 1104 42-43 -5.110097882853303 1104 44-45 -7.093928761297185 1104 46-47 -0.8680639209431718 1104 48-49 -6.410288019514027 1104 50-51 -0.6935561497326148 1104 52-53 -8.88582090252369 1104 54-55 -2.9881697782781345 1104 56-57 -2.2810512243174763 1104 58-59 -6.66876411170529 1104 60-61 -14.789476498733464 1104 62-63 -14.273098007943208 1104 64-65 -14.668614629264784 1104 66-67 -13.622999499640361 1104 68-69 -13.387899271351285 1104 70-71 -12.695514432248176 1104 72-73 -11.908048753791782 1104 74-75 -11.981236357381869 1104 76-77 -12.086055758826658 1104 78-79 -11.699643337398756 1104 80-81 -11.341489351721552 1104 82-83 -9.990316790192953 1104 84-85 -9.786322982143414 1104 86-87 -9.66788394783751 1104 88-89 -8.989720424054791 1104 90-91 -8.590725834193327 1104 92-93 -7.958230134158928 1104 94-95 -7.9887411264346255 1104 96-97 -7.212589517465677 1104 98-99 -6.3676157863464375 1104 100-101 -5.719169246646027 1105 1 11.166627576070303 1105 2 5.340012508990842 1105 3 2.889550301779412 1105 4 0.31667667385934095 1105 5 0.8557175469868952 1105 6 1.4618816649466773 1105 7 1.6756005879225704 1105 8 1.538603371173025 1105 9 -1.5458198079869945 1105 10-11 1.26413390874691 1105 12-13 -1.6827363417456347 1105 14-15 1.8352581542984048 1105 16-17 3.3518969884604566 1105 18-19 -0.31494058229352717 1105 20-21 1.0090061606779912 1105 22-23 1.9617074772492735 1105 24-25 3.601804265565878 1105 26-27 2.8444779059949283 1105 28-29 4.476374269005852 1105 30-31 3.6198108015135837 1105 32-33 5.413408856365518 1105 34-35 3.7281394439753583 1105 36-37 4.748034681177092 1105 38-39 2.537928511117368 1105 40-41 5.342300403414949 1105 42-43 0.8065687838133648 1105 44-45 0.7394045720361468 1105 46-47 3.0486027457234925 1105 48-49 6.589711980485973 1105 50-51 5.056443850267385 1105 52-53 6.11417909747631 1105 54-55 6.345163555055201 1105 56-57 4.218948775682524 1105 58-59 5.08123588829471 1105 60-61 6.543856834599868 1105 62-63 -2.273098007943208 1105 64-65 4.831385370735216 1105 66-67 8.210333833692975 1105 68-69 6.94543406198205 1105 70-71 6.63781890108516 1105 72-73 1.2586179128748842 1105 74-75 1.018763642618131 1105 76-77 -3.0860557588266584 1105 78-79 0.6336899959345779 1105 80-81 0.15851064827844752 1105 82-83 6.343016543140383 1105 84-85 2.380343684523254 1105 86-87 0.9987827188291583 1105 88-89 -0.3230537573881236 1105 90-91 -5.257392500859994 1105 92-93 0.875103199174406 1105 94-95 1.3445922068987084 1105 96-97 1.620743815867657 1105 98-99 3.1323842136535625 1105 100-101 4.114164086687305 1106 1 19.499960909403633 1106 2 13.50667917565751 1106 3 11.55621696844608 1106 4 13.316676673859341 1106 5 12.189050880320227 1106 6 13.128548331613345 1106 7 13.342267254589238 1106 8 13.205270037839693 1106 9 12.787513525346338 1106 10-11 12.680800575413578 1106 12-13 12.400596991587701 1106 14-15 12.335258154298405 1106 16-17 14.435230321793792 1106 18-19 13.935059417706473 1106 20-21 14.009006160677991 1106 22-23 13.461707477249274 1106 24-25 14.185137598899214 1106 26-27 14.761144572661596 1106 28-29 12.893040935672516 1106 30-31 10.119810801513584 1106 32-33 14.163408856365518 1106 34-35 14.561472777308694 1106 36-37 10.581368014510428 1106 38-39 6.8712618444507 1106 40-41 12.675633736748285 1106 42-43 6.889902117146697 1106 44-45 1.1560712387028147 1106 46-47 -5.868063920943172 1106 48-49 -0.9102880195140273 1106 50-51 -10.193556149732615 1106 52-53 -6.63582090252369 1106 54-55 -3.2381697782781345 1106 56-57 -1.7810512243174763 1106 58-59 -8.16876411170529 1106 60-61 -14.789476498733464 1106 62-63 -14.273098007943208 1106 64-65 -14.668614629264784 1106 66-67 -13.622999499640361 1106 68-69 -13.387899271351285 1106 70-71 -12.695514432248176 1106 72-73 -11.908048753791782 1106 74-75 -11.981236357381869 1106 76-77 -12.086055758826658 1106 78-79 -11.699643337398756 1106 80-81 -11.341489351721552 1106 82-83 -9.990316790192953 1106 84-85 -9.786322982143414 1106 86-87 -9.66788394783751 1106 88-89 -8.989720424054791 1106 90-91 -8.590725834193327 1106 92-93 -7.958230134158928 1106 94-95 -7.9887411264346255 1106 96-97 -7.212589517465677 1106 98-99 -6.3676157863464375 1106 100-101 -5.719169246646027 1107 1 17.999960909403633 1107 2 15.00667917565751 1107 3 12.55621696844608 1107 4 5.816676673859341 1107 5 4.189050880320227 1107 6 2.128548331613345 1107 7 -0.6577327454107618 1107 8 -1.2947299621603072 1107 9 0.2875135253463377 1107 10-11 5.680800575413578 1107 12-13 3.400596991587701 1107 14-15 2.0852581542984048 1107 16-17 8.185230321793792 1107 18-19 10.935059417706473 1107 20-21 2.009006160677991 1107 22-23 1.7117074772492735 1107 24-25 11.685137598899214 1107 26-27 12.261144572661596 1107 28-29 12.143040935672516 1107 30-31 12.619810801513584 1107 32-33 11.413408856365518 1107 34-35 8.811472777308694 1107 36-37 6.831368014510428 1107 38-39 13.1212618444507 1107 40-41 -0.324366263251715 1107 42-43 -1.110097882853303 1107 44-45 -1.0939287612971853 1107 46-47 0.38193607905682825 1107 48-49 1.0897119804859727 1107 50-51 2.806443850267385 1107 52-53 3.3641790974763097 1107 54-55 0.7618302217218655 1107 56-57 1.7189487756825237 1107 58-59 2.58123588829471 1107 60-61 1.460523501266536 1107 62-63 -0.7730980079432079 1107 64-65 -1.668614629264784 1107 66-67 -0.12299949964036117 1107 68-69 2.6121007286487146 1107 70-71 -3.195514432248176 1107 72-73 -5.408048753791782 1107 74-75 0.768763642618131 1107 76-77 2.4139442411733416 1107 78-79 2.800356662601244 1107 80-81 -3.5914893517215525 1107 82-83 -9.990316790192953 1107 84-85 -9.786322982143414 1107 86-87 -9.66788394783751 1107 88-89 -8.989720424054791 1107 90-91 -8.590725834193327 1107 92-93 -7.958230134158928 1107 94-95 -7.9887411264346255 1107 96-97 -7.212589517465677 1107 98-99 -6.3676157863464375 1107 100-101 -5.719169246646027 1108 1 10.499960909403635 1108 2 4.00667917565751 1108 3 1.889550301779412 1108 4 -3.349989992807327 1108 5 0.18905088032022732 1108 6 -2.204785001719987 1108 7 1.008933921255906 1108 8 0.8719367045063606 1108 9 1.7875135253463377 1108 10-11 1.097467242080242 1108 12-13 -0.5160696750789633 1108 14-15 0.8352581542984048 1108 16-17 -3.981436344872872 1108 18-19 -0.8149405822935272 1108 20-21 -1.8243271726553445 1108 22-23 -0.8716258560840622 1108 24-25 2.435137598899214 1108 26-27 2.344477905994932 1108 28-29 2.1430409356725164 1108 30-31 1.9531441348469158 1108 32-33 3.5800755230321855 1108 34-35 3.3948061106420298 1108 36-37 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92-93 18.541769865841072 2313 94-95 21.761258873565374 2313 96-97 21.787410482534323 2313 98-99 17.132384213653562 2313 100-101 12.530830753353973 2314 1 -9.500039090596365 2314 2 -2.99332082434249 2314 3 -0.4437830315539202 2314 4 4.983343340526009 2314 5 9.855717546986892 2314 6 -0.5381183350533227 2314 7 -2.6577327454107618 2314 8 -0.4613966288269751 2314 9 2.12084685867967 2314 10-11 7.597467242080246 2314 12-13 1.650596991587701 2314 14-15 5.835258154298405 2314 16-17 7.018563655127124 2314 18-19 10.518392751039801 2314 20-21 2.009006160677991 2314 22-23 4.628374143915938 2314 24-25 3.7684709322325496 2314 26-27 0.5111445726615962 2314 28-29 -1.5236257309941479 2314 30-31 6.619810801513584 2314 32-33 4.413408856365518 2314 34-35 3.728139443975362 2314 36-37 6.248034681177092 2314 38-39 6.3712618444507 2314 40-41 7.675633736748285 2314 42-43 4.806568783813361 2314 44-45 7.072737905369479 2314 46-47 7.5486027457234925 2314 48-49 10.25637864715264 2314 50-51 8.056443850267385 2314 52-53 5.61417909747631 2314 54-55 11.011830221721866 2314 56-57 8.05228210901586 2314 58-59 6.58123588829471 2314 60-61 -2.289476498733464 2314 62-63 -1.9397646746098758 2314 64-65 -1.668614629264784 2314 66-67 -0.789666166307029 2314 68-69 -1.3878992713512854 2314 70-71 -1.195514432248176 2314 72-73 -1.574715420458448 2314 74-75 -1.481236357381869 2314 76-77 -0.41938909215999054 2314 78-79 -0.6996433373987561 2314 80-81 -0.8414893517215525 2314 82-83 1.5096832098070472 2314 84-85 -0.7863229821434139 2314 86-87 -2.0012172811708417 2314 88-89 0.17694624261187641 2314 90-91 0.9092741658066732 2314 92-93 1.2084365325077382 2314 94-95 3.0112588735653745 2314 96-97 1.1207438158676553 2314 98-99 3.4657175469868946 2314 100-101 2.614164086687305 2315 1 -9.500039090596365 2315 2 -14.99332082434249 2315 3 -17.44378303155392 2315 4 -21.68332332614066 2315 5 -21.810949119679773 2315 6 -21.871451668386655 2315 7 -21.65773274541076 2315 8 -21.794729962160307 2315 9 -22.212486474653662 2315 10-11 -21.569199424586422 2315 12-13 -21.8494030084123 2315 14-15 -21.664741845701595 2315 16-17 -21.314769678206208 2315 18-19 -22.314940582293527 2315 20-21 -21.49099383932201 2315 22-23 -20.538292522750726 2315 24-25 -21.064862401100786 2315 26-27 -20.488855427338404 2315 28-29 -19.856959064327484 2315 30-31 -19.880189198486416 2315 32-33 -19.086591143634482 2315 34-35 -18.938527222691306 2315 36-37 -18.418631985489572 2315 38-39 -18.6287381555493 2315 40-41 -18.324366263251715 2315 42-43 -17.860097882853303 2315 44-45 -17.093928761297185 2315 46-47 -16.61806392094317 2315 48-49 -16.410288019514027 2315 50-51 -15.943556149732615 2315 52-53 -15.88582090252369 2315 54-55 -15.988169778278134 2315 56-57 -16.281051224317476 2315 58-59 -15.41876411170529 2315 60-61 -14.789476498733464 2315 62-63 -14.273098007943208 2315 64-65 -14.668614629264784 2315 66-67 -13.622999499640361 2315 68-69 -13.387899271351285 2315 70-71 -12.695514432248176 2315 72-73 -11.908048753791782 2315 74-75 -11.981236357381869 2315 76-77 -12.086055758826658 2315 78-79 -11.699643337398756 2315 80-81 -11.341489351721552 2315 82-83 -9.990316790192953 2315 84-85 -9.786322982143414 2315 86-87 -9.66788394783751 2315 88-89 -8.989720424054791 2315 90-91 -8.590725834193327 2315 92-93 -7.958230134158928 2315 94-95 -7.9887411264346255 2315 96-97 -7.212589517465677 2315 98-99 -6.3676157863464375 2315 100-101 -5.719169246646027 2316 1 -9.500039090596365 2316 2 -10.99332082434249 2316 3 -13.44378303155392 2316 4 -17.68332332614066 2316 5 -13.310949119679773 2316 6 -8.871451668386655 2316 7 -17.65773274541076 2316 8 -7.294729962160307 2316 9 -18.212486474653662 2316 10-11 -15.569199424586422 2316 12-13 -11.849403008412299 2316 14-15 -17.414741845701595 2316 16-17 -8.814769678206208 2316 18-19 -7.814940582293527 2316 20-21 -17.49099383932201 2316 22-23 -12.288292522750726 2316 24-25 -4.814862401100786 2316 26-27 -14.488855427338404 2316 28-29 -15.856959064327484 2316 30-31 -8.130189198486416 2316 32-33 -15.836591143634482 2316 34-35 -13.688527222691306 2316 36-37 -5.918631985489572 2316 38-39 -4.6287381555493 2316 40-41 -7.324366263251715 2316 42-43 -8.360097882853303 2316 44-45 -9.593928761297185 2316 46-47 -11.618063920943172 2316 48-49 -3.9102880195140273 2316 50-51 0.8064438502673852 2316 52-53 -9.88582090252369 2316 54-55 -11.238169778278134 2316 56-57 -10.031051224317476 2316 58-59 -12.41876411170529 2316 60-61 -11.789476498733464 2316 62-63 -7.523098007943208 2316 64-65 -1.918614629264784 2316 66-67 1.6270005003596388 2316 68-69 1.3621007286487146 2316 70-71 -6.945514432248176 2316 72-73 -4.158048753791782 2316 74-75 1.018763642618131 2316 76-77 1.1639442411733416 2316 78-79 -0.9496433373987561 2316 80-81 5.4085106482784475 2316 82-83 -9.990316790192953 2316 84-85 -9.786322982143414 2316 86-87 -9.66788394783751 2316 88-89 -8.989720424054791 2316 90-91 -8.590725834193327 2316 92-93 -7.958230134158928 2316 94-95 -7.9887411264346255 2316 96-97 -7.212589517465677 2316 98-99 -6.3676157863464375 2316 100-101 -5.719169246646027 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 506.0 3 67.0 4 55.0 5 58.0 6 52.0 7 42.0 8 50.0 9 47.0 10 43.0 11 42.0 12 37.0 13 57.0 14 45.0 15 51.0 16 62.0 17 53.0 18 40.0 19 49.0 20 48.0 21 56.0 22 57.0 23 82.0 24 56.0 25 63.0 26 93.0 27 73.0 28 105.0 29 108.0 30 104.0 31 120.0 32 107.0 33 116.0 34 111.0 35 96.0 36 82.0 37 28.0 38 3.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.20631067961165 19.053398058252426 13.652912621359222 22.0873786407767 2 16.13691931540342 21.830247991617185 40.447083478868315 21.585749214111072 3 21.76039119804401 28.082431016416347 28.676213761788333 21.48096402375131 4 13.377575969263011 16.45127488648271 39.259517988124344 30.911631156129932 5 14.285714285714285 39.50401676563045 32.27383863080684 13.93643031784841 6 29.898707649318894 35.59203632553265 19.42018861334265 15.089067411805798 7 28.780998952148096 33.70590289905693 21.306322039818372 16.206776108976598 8 26.685295144952846 32.972406566538595 20.677610897659797 19.66468739084876 9 28.441649196366175 18.448637316561843 20.999301187980432 32.11041229909154 10-11 25.480265455815577 26.8075445337059 27.96018162766329 19.752008382815227 12-13 28.449179182675515 25.165909884736294 26.737687740132728 19.647223192455463 14-15 24.065665385958784 26.772616136919314 26.737687740132728 22.424030736989174 16-17 22.882096069868997 28.034934497816593 28.104803493449783 20.978165938864628 18-19 22.0125786163522 29.629629629629626 28.476589797344516 19.881201956673657 20-21 23.916841369671555 28.127183787561144 26.95667365478686 20.999301187980432 22-23 22.789234533379936 27.31562390772457 27.368053128276827 22.527088430618665 24-25 22.74077958398881 27.530152071316206 27.77486453417235 21.954203810522635 26-27 24.764068507514857 26.77385529535127 27.927298147500874 20.534778049632997 28-29 22.719328905976933 26.77385529535127 29.86717930793429 20.639636490737505 30-31 23.4224785876595 28.64883761580143 28.229330536619475 19.699353259919594 32-33 23.5232436211115 28.049632995456136 27.42048234882908 21.006641034603284 34-35 23.76791331702202 27.210765466620064 27.630199231038098 21.39112198531982 36-37 23.571553381093832 27.642844661890614 27.590424602481217 21.195177354534337 38-39 23.602375960866524 27.568134171907754 27.72536687631027 21.104122990915446 40-41 23.724668064290704 27.707896575821106 27.620545073375265 20.94689028651293 42-43 22.79874213836478 27.882599580712785 27.498252969951082 21.82040531097135 44-45 23.5366066748209 27.765158133845887 26.89149047702254 21.80674471431068 46-47 21.862984970290107 28.801118490038448 27.036001398112546 22.299895141558896 48-49 24.01258301293254 27.385529535127578 26.00489339391821 22.59699405802167 50-51 24.593602517042477 27.337877993357807 26.62122006642195 21.447299423177764 52-53 23.561308378520202 28.179114920412808 26.867238061920588 21.392338639146406 54-55 21.97936702220668 28.22171708340619 27.03269802413009 22.766217870257037 56-57 24.501922404753582 27.455435162530584 26.45927997203775 21.583362460678085 58-59 23.602375960866524 27.148846960167717 26.397624039133472 22.851153039832283 60-61 22.44933612858141 27.166317260656886 27.795248078266948 22.58909853249476 62-63 24.21383647798742 27.096436058700206 26.170510132774282 22.519217330538087 64-65 23.58490566037736 28.232005590496158 26.397624039133472 21.785464709993015 66-67 23.095737246680642 28.039832285115303 27.096436058700206 21.767994409503842 68-69 22.90356394129979 29.996505939902168 25.821104122990917 21.278825995807125 70-71 23.68972746331237 27.830188679245282 24.3361285814116 24.14395527603075 72-73 23.396819849729162 27.660318015027087 25.318888694740522 23.62397344050323 74-75 23.308270676691727 28.379087253016262 25.72127994404616 22.591362126245848 76-77 23.776223776223777 27.307692307692307 26.206293706293703 22.70979020979021 78-79 22.911569381335198 28.69626004893394 25.98741698706746 22.404753582663403 80-81 22.92904578818595 29.098217406501227 25.026214610276128 22.9465221950367 82-83 23.387519664394336 28.491522461108197 25.100506904387345 23.020450970110122 84-85 21.837875611460518 28.162124388539482 25.838574423480082 24.161425576519914 86-87 22.833682739343118 28.441649196366175 25.960866526904265 22.763801537386446 88-89 22.92103424178896 27.900069881201954 25.174703004891686 24.0041928721174 90-91 23.095737246680642 26.502445842068482 26.572327044025158 23.829489867225718 92-93 23.934311670160728 27.77777777777778 25.663871418588403 22.624039133473094 94-95 23.090368816640446 27.49519314805104 25.537493445201886 23.876944590106625 96-97 22.263029031129765 27.26477789436866 26.33788037775446 24.134312696747116 98-99 21.990902729181247 27.904128761371588 25.64730580825752 24.45766270118964 100-101 22.966590869337065 28.056673080286863 25.275494140283367 23.701241910092705 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3.0 1 3.5 2 2.0 3 0.5 4 1.0 5 1.0 6 1.5 7 3.5 8 4.0 9 3.5 10 4.0 11 3.5 12 1.5 13 1.5 14 5.0 15 5.0 16 3.0 17 3.0 18 4.0 19 4.5 20 5.5 21 5.0 22 4.0 23 6.5 24 6.0 25 6.5 26 12.0 27 17.0 28 22.5 29 26.5 30 31.5 31 35.5 32 40.5 33 50.5 34 53.5 35 58.5 36 70.5 37 73.5 38 71.0 39 73.5 40 81.5 41 83.0 42 81.0 43 97.5 44 108.0 45 101.0 46 100.5 47 113.0 48 140.0 49 144.0 50 129.0 51 115.5 52 102.0 53 90.5 54 85.0 55 82.5 56 77.5 57 68.5 58 63.0 59 54.0 60 36.5 61 35.0 62 35.0 63 31.5 64 25.0 65 16.0 66 12.5 67 9.0 68 4.0 69 3.5 70 3.0 71 2.0 72 2.5 73 2.5 74 1.0 75 0.5 76 0.5 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 42.45810055865922 2 0.034916201117318434 3 0.034916201117318434 4 0.034916201117318434 5 0.034916201117318434 6 0.034916201117318434 7 0.034916201117318434 8 0.034916201117318434 9 0.06983240223463687 10-11 0.034916201117318434 12-13 0.034916201117318434 14-15 0.034916201117318434 16-17 0.052374301675977654 18-19 0.06983240223463687 20-21 0.06983240223463687 22-23 0.10474860335195531 24-25 0.12220670391061453 26-27 0.10474860335195531 28-29 0.10474860335195531 30-31 0.12220670391061453 32-33 0.10474860335195531 34-35 0.10474860335195531 36-37 0.08729050279329609 38-39 0.06983240223463687 40-41 0.06983240223463687 42-43 0.06983240223463687 44-45 0.08729050279329609 46-47 0.10474860335195531 48-49 0.10474860335195531 50-51 0.12220670391061453 52-53 0.19203910614525138 54-55 0.15712290502793297 56-57 0.10474860335195531 58-59 0.06983240223463687 60-61 0.06983240223463687 62-63 0.06983240223463687 64-65 0.06983240223463687 66-67 0.06983240223463687 68-69 0.06983240223463687 70-71 0.06983240223463687 72-73 0.08729050279329609 74-75 0.15712290502793297 76-77 0.13966480446927373 78-79 0.10474860335195531 80-81 0.10474860335195531 82-83 0.12220670391061453 84-85 0.06983240223463687 86-87 0.06983240223463687 88-89 0.06983240223463687 90-91 0.06983240223463687 92-93 0.06983240223463687 94-95 0.12220670391061453 96-97 0.17458100558659218 98-99 0.20949720670391062 100-101 0.19203910614525138 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2864.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 96.54329608938548 #Duplication Level Percentage of deduplicated Percentage of total 1 98.51717902350813 95.11173184357543 2 0.8318264014466547 1.606145251396648 3 0.18083182640144665 0.5237430167597765 4 0.216998191681736 0.8379888268156425 5 0.0 0.0 6 0.108499095840868 0.6284916201117319 7 0.0 0.0 8 0.07233273056057866 0.5586592178770949 9 0.03616636528028933 0.31424581005586594 >10 0.03616636528028933 0.41899441340782123 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 12 0.41899441340782123 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 9 0.31424581005586594 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8 0.27932960893854747 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 8 0.27932960893854747 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 6 0.20949720670391062 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 6 0.20949720670391062 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 6 0.20949720670391062 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4 0.13966480446927373 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 4 0.13966480446927373 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 4 0.13966480446927373 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 4 0.13966480446927373 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4 0.13966480446927373 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 4 0.13966480446927373 No Hit AAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTAC 3 0.10474860335195531 No Hit NTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3 0.10474860335195531 No Hit NAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 3 0.10474860335195531 No Hit NTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGC 3 0.10474860335195531 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 3 0.10474860335195531 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.05237430167597765 0.0 38-39 0.0 0.0 0.0 0.08729050279329609 0.0 40-41 0.0 0.0 0.0 0.13966480446927373 0.0 42-43 0.0 0.0 0.0 0.20949720670391062 0.0 44-45 0.0 0.0 0.0 0.26187150837988826 0.0 46-47 0.0 0.0 0.0 0.27932960893854747 0.0 48-49 0.0 0.0 0.0 0.3840782122905028 0.0 50-51 0.0 0.0 0.0 0.3840782122905028 0.0 52-53 0.0 0.0 0.0 0.401536312849162 0.0 54-55 0.0 0.0 0.0 0.47136871508379885 0.0 56-57 0.0 0.0 0.0 0.5586592178770949 0.0 58-59 0.0 0.0 0.0 0.6284916201117319 0.0 60-61 0.0 0.0 0.0 0.8205307262569832 0.0 62-63 0.0 0.0 0.0 0.9427374301675977 0.0 64-65 0.0 0.0 0.0 1.1696927374301676 0.0 66-67 0.0 0.0 0.0 1.204608938547486 0.0 68-69 0.0 0.0 0.0 1.3966480446927374 0.0 70-71 0.0 0.0 0.0 1.5537709497206704 0.0 72-73 0.0 0.0 0.0 1.6585195530726258 0.0 74-75 0.0 0.0 0.0 1.9553072625698324 0.0 76-77 0.0 0.0 0.0 2.147346368715084 0.0 78-79 0.0 0.0 0.0 2.4266759776536313 0.0 80-81 0.0 0.0 0.0 2.7409217877094973 0.0 82-83 0.0 0.0 0.0 2.985335195530726 0.0 84-85 0.0 0.0 0.0 3.177374301675978 0.0 86-87 0.0 0.0 0.0 3.4741620111731844 0.0 88-89 0.0 0.0 0.0 3.5789106145251397 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE