FastQCFastQC Report
Fri 27 May 2016
SRR939648_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939648_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2864
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA200.6983240223463687No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA200.6983240223463687No Hit
TGCTTACACCACATGAAACACTGTCTCTTCTATAGGATCATTTATTTCAC180.6284916201117319No Hit
CTCTTCTATAGGATCATTTATTTCACTAACAGCTGTTCTCATCATGATCT120.41899441340782123No Hit
ACATGAAACACTGTCTCTTCTATAGGATCATTTATTTCACTAACAGCTGT120.41899441340782123No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110.38407821229050276No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT110.38407821229050276No Hit
ACTCAGACTACCCAGATGCTTACACCACATGAAACACTGTCTCTTCTATA90.31424581005586594No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT80.27932960893854747No Hit
CTTCTATAGGATCATTTATTTCACTAACAGCTGTTCTCATCATGATCTTT80.27932960893854747No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80.27932960893854747No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT70.24441340782122906No Hit
GTTCTCATCATGATCTTTATAATTTGAGAGGCCTTTGCTTCAAAACGAGA70.24441340782122906No Hit
TCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCTTG70.24441340782122906TruSeq Adapter, Index 1 (96% over 28bp)
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC60.20949720670391062No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT60.20949720670391062No Hit
ACGTAACACAGTTCCACCCGCCTCACATTGAAATCCAAATGCTGAAGAAC60.20949720670391062No Hit
TCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCTTG60.20949720670391062RNA PCR Primer, Index 33 (96% over 29bp)
ATACTATGTAGTAGCCCATTTCCACTATGTTCTATCAATGGGAGCAGTGT60.20949720670391062No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA60.20949720670391062No Hit
TCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCTTG60.20949720670391062RNA PCR Primer, Index 26 (100% over 29bp)
TCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCTTG60.20949720670391062RNA PCR Primer, Index 31 (96% over 29bp)
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG50.17458100558659218No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT50.17458100558659218No Hit
GTTCCACCCGCCTCACATTGAAATCCAAATGCTGAAGAACGGGAAAAAAA50.17458100558659218No Hit
ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA50.17458100558659218No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT50.17458100558659218No Hit
ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG40.13966480446927373No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40.13966480446927373No Hit
ACCCAGATGCTTACACCACATGAAACACTGTCTCTTCTATAGGATCATTT40.13966480446927373No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40.13966480446927373No Hit
TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG40.13966480446927373No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA40.13966480446927373No Hit
GCTACGTAACACAGTTCCACCCGCCTCACATTGAAATCCAAATGCTGAAG40.13966480446927373No Hit
ACACAGTTCCACCCGCCTCACATTGAAATCCAAATGCTGAAGAACGGGAA40.13966480446927373No Hit
GTAGTAGCCCATTTCCACTATGTTCTATCAATGGGAGCAGTGTTTGCTAT30.10474860335195531No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30.10474860335195531No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCT30.10474860335195531No Hit
CTATAGGATCATTTATTTCACTAACAGCTGTTCTCATCATGATCTTTATA30.10474860335195531No Hit
GGAATACCACGACGCTACTCAGACTACCCAGATGCTTACACCACATGAAA30.10474860335195531No Hit
CTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCTTGA30.10474860335195531RNA PCR Primer, Index 33 (96% over 29bp)
GCCTGTATGCTATCCAGAAAACCCCTCAAATTCAAGTATACTCACGCCAC30.10474860335195531No Hit
TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC30.10474860335195531No Hit
CAGGAATACCACGACGCTACTCAGACTACCCAGATGCTTACACCACATGA30.10474860335195531No Hit
TCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTA30.10474860335195531No Hit
CTCATATACACCGAGAAAATCAAGTAAGTAGCAGAAAATGGTGTCTTAGA30.10474860335195531No Hit
AAACATAACATTCTTCCCTCAACATTTCCTGGGCCTTTCAGGAATACCAC30.10474860335195531No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA30.10474860335195531No Hit
GGATCATTTATTTCACTAACAGCTGTTCTCATCATGATCTTTATAATTTG30.10474860335195531No Hit
GAGTAAACATAACATTCTTCCCTCAACATTTCCTGGGCCTTTCAGGAATA30.10474860335195531No Hit
CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT30.10474860335195531No Hit
ACTAACAGCTGTTCTCATCATGATCTTTATAATTTGAGAGGCCTTTGCTT30.10474860335195531No Hit
ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT30.10474860335195531No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT30.10474860335195531No Hit
GTTAATATCCATATTAAATGCTGATAATATAAACTCTCCAAAGGGTACAA30.10474860335195531No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG30.10474860335195531No Hit
GTGTTTGCTATCATAGCAGGATTTGTTCACTGATTCCCATTATTTTCAGG30.10474860335195531No Hit
CCACTGTACCGTATGCGAATCTTTGCACCCAACCATGTGGTGGCCAAGTC30.10474860335195531No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC30.10474860335195531No Hit
CTCCAAAGGGTACAACTGAGAATAGAAAAAAGCAGAACTTCTGCATCTAA30.10474860335195531No Hit
CTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCTTGA30.10474860335195531RNA PCR Primer, Index 1 (96% over 28bp)
GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA30.10474860335195531No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCCCAC201.4613079E-595.000017
GCCCACG201.4613079E-595.000018
GAGCCCA201.4613079E-595.000016
CCCACGA201.4613079E-595.000019
CGAGCCC201.4613079E-595.000015
TCCGAGC201.4613079E-595.000013
CCGAGCC254.4240252E-576.04
TCTCCGA254.4240252E-576.01
CTCCGAG254.4240252E-576.02
TGCCGTC204.947001E-447.50000434-35
CCACGAG204.947001E-447.50000410-11
TATGCCG204.947001E-447.50000432-33
CCGTCTT204.947001E-447.50000436-37
CACGAGA204.947001E-447.50000410-11
ACGAGAC204.947001E-447.50000412-13
ATGCCGT204.947001E-447.50000434-35
GTCTTCT204.947001E-447.50000438-39
GCCGTCT204.947001E-447.50000436-37
CTTGAAA204.947001E-447.50000446-47
TATCTCG150.00980996647.526-27
Produced by FastQC (version 0.11.5)