FastQCFastQC Report
Fri 27 May 2016
SRR939647_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939647_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2645913
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA177470.6707325599896898No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA135600.5124885058579024No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT117750.44502597024165197No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT115450.4363333185936197No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79360.2999342759947134No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT64960.24551071785051132No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA61350.2318670341768607No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT60860.2300151214344538No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55900.21126922918478422No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG54970.2077543743879712No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT54130.20457966682955941No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT47790.18061818359107046No Hit
GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG44740.16909097162302766No Hit
TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC44520.1682595005958246No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC43630.16489582234941208No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT35070.132544040563692No Hit
TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG31940.1207144754948481No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT28710.10850696905000277No Hit
GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT26600.10053240601637317No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG167650.028.5616381
TACATGG165850.028.385722
ACATGGG169350.026.5099953
CATGGGA123050.024.1033524
CATGGGG46750.023.2451784
GAGTACT95700.021.74386412-13
ATGGGAT46500.021.7373055
ATGGGAG45800.021.4478575
AGTACTT99000.021.33063912-13
TAGGACG3201.8189894E-1120.7614214
GTACTTT106000.019.72167214-15
CGTATAC1503.4870027E-919.10130990-91
AGTACAT120100.017.2680032
ATGGGGG26000.017.156695
GCGTATC1952.2927162E-417.0356581
ATGGGGA25150.016.9817925
ACTTTTT132850.016.89723416-17
TACTTTT127150.016.62780814-15
TAAAGCG3452.1229425E-816.5059745
GCTTATA27250.016.1961561
Produced by FastQC (version 0.11.5)