##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939647_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2645913 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.211807795645587 31.0 30.0 33.0 26.0 34.0 2 30.325466105650488 31.0 30.0 34.0 26.0 34.0 3 30.377904337746553 31.0 30.0 34.0 26.0 34.0 4 33.94866649054599 35.0 35.0 37.0 28.0 37.0 5 33.96803258459367 35.0 35.0 37.0 28.0 37.0 6 34.09629114789489 36.0 35.0 37.0 29.0 37.0 7 33.90837680604011 36.0 35.0 37.0 28.0 37.0 8 33.9192433764829 36.0 35.0 37.0 28.0 37.0 9 35.14690770255863 38.0 35.0 39.0 28.0 39.0 10-11 35.048330198309614 37.0 34.0 39.0 28.0 39.0 12-13 34.901422306780304 37.0 34.0 39.0 27.0 39.0 14-15 35.52222446467439 38.0 34.0 40.0 27.0 41.0 16-17 35.29891364530882 38.0 34.0 40.0 27.0 41.0 18-19 35.342249348334576 38.0 34.0 40.0 27.0 41.0 20-21 35.4810766642743 38.0 34.0 40.0 27.0 41.0 22-23 35.355952557774955 38.0 34.0 40.0 27.0 41.0 24-25 35.22192434142771 38.0 34.0 40.0 26.0 41.0 26-27 35.31539113341973 38.0 34.0 40.0 26.5 41.0 28-29 35.19508918093679 38.0 34.0 40.0 25.0 41.0 30-31 34.983705624485765 38.0 34.0 40.0 25.0 41.0 32-33 34.751496931305 38.0 33.5 40.0 24.5 41.0 34-35 34.51848908108468 38.0 33.0 40.0 24.0 41.0 36-37 34.187522416647866 38.0 33.0 40.0 24.0 41.0 38-39 33.999307233457785 38.0 33.0 40.0 23.5 41.0 40-41 33.718476382254444 38.0 32.5 40.0 21.5 41.0 42-43 33.40901496005348 37.5 32.0 40.0 19.0 41.0 44-45 33.143921020834775 37.0 31.5 40.0 18.0 41.0 46-47 32.64369255527298 37.0 31.0 40.0 14.5 41.0 48-49 32.261293549712335 37.0 30.5 40.0 11.0 41.0 50-51 31.62939843449123 36.0 30.0 38.5 9.0 40.5 52-53 31.63842046204845 36.5 30.0 39.0 8.5 40.0 54-55 31.692555272981387 36.0 30.0 39.0 8.0 40.0 56-57 31.589456456051277 36.0 30.0 39.0 7.0 40.0 58-59 31.21301909775567 36.0 29.0 39.0 4.0 40.0 60-61 30.768112179047456 35.5 28.5 39.0 2.0 40.0 62-63 30.33982409852478 35.0 28.0 38.5 2.0 40.0 64-65 29.69034904019898 34.5 27.5 38.0 2.0 40.0 66-67 29.14834047831505 34.0 26.0 38.0 2.0 40.0 68-69 28.658049414323145 34.0 26.0 37.0 2.0 39.0 70-71 28.073520746902865 33.5 26.0 36.5 2.0 39.0 72-73 27.311790296959877 32.5 24.5 36.0 2.0 38.5 74-75 26.8349858819999 32.0 23.0 35.0 2.0 38.0 76-77 26.431352051257925 32.0 22.5 35.0 2.0 37.5 78-79 25.85123471557833 31.5 20.5 35.0 2.0 37.0 80-81 25.188425129624445 31.0 19.0 34.5 2.0 36.0 82-83 24.52993692536376 31.0 16.5 34.0 2.0 36.0 84-85 23.87107814958391 30.0 9.0 34.0 2.0 35.0 86-87 23.177852219630804 30.0 4.5 34.0 2.0 35.0 88-89 22.328027603326337 29.0 2.0 34.0 2.0 35.0 90-91 21.08428149376038 28.0 2.0 33.5 2.0 35.0 92-93 19.511419120734505 25.5 2.0 32.5 2.0 34.0 94-95 19.238988772495542 25.0 2.0 32.0 2.0 34.0 96-97 18.56188128634615 25.0 2.0 32.0 2.0 34.0 98-99 17.56909089603475 23.5 2.0 32.0 2.0 34.0 100-101 15.94176622587364 10.0 2.0 31.0 2.0 34.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.9269733090968124 1101 2 1.0216307471431811 1101 3 0.9811955510659729 1101 4 1.17242278148521 1101 5 1.0006039282564458 1101 6 0.8754897285142818 1101 7 0.8126670550121062 1101 8 1.1612722108043698 1101 9 1.2136848631392851 1101 10-11 1.053710324129554 1101 12-13 1.1032827637171962 1101 14-15 1.2715436853830866 1101 16-17 1.2018236990365736 1101 18-19 1.1059797872800203 1101 20-21 1.3213099545955416 1101 22-23 1.463715119271555 1101 24-25 1.370173140928408 1101 26-27 1.3814056535416697 1101 28-29 1.318971633576048 1101 30-31 1.5199389462642685 1101 32-33 1.3731490081264681 1101 34-35 1.335100078772598 1101 36-37 1.268975177263421 1101 38-39 1.2555119880878323 1101 40-41 1.285001678367145 1101 42-43 1.2747782083915453 1101 44-45 1.387744728928027 1101 46-47 1.2329110430705228 1101 48-49 1.11779007559133 1101 50-51 1.236827326575252 1101 52-53 1.3767298472674234 1101 54-55 1.33026657135634 1101 56-57 1.2021537843483507 1101 58-59 1.1174070040718966 1101 60-61 0.8404061020691103 1101 62-63 0.9548330758896846 1101 64-65 0.9846565626189765 1101 66-67 0.8000352247060327 1101 68-69 0.748011380697843 1101 70-71 0.9111945231311473 1101 72-73 1.0119109789337841 1101 74-75 0.7282654817941214 1101 76-77 0.2777696778559928 1101 78-79 0.4938299327915914 1101 80-81 0.413803306145649 1101 82-83 0.25226352512979844 1101 84-85 -0.05794744857498202 1101 86-87 -0.5449574240720771 1101 88-89 -0.913451498682111 1101 90-91 -4.4399629172119255 1101 92-93 -9.756065831427005 1101 94-95 -8.033255555267363 1101 96-97 -4.49709382507945 1101 98-99 -3.2340615733117204 1101 100-101 -2.876727675026059 1102 1 1.1427768852355165 1102 2 1.1832845759848176 1102 3 1.1557400726157745 1102 4 1.2214228972688517 1102 5 1.220079010452551 1102 6 1.1134233947655403 1102 7 1.160929169988684 1102 8 0.9959071395083683 1102 9 1.3283945921850204 1102 10-11 1.109382557774083 1102 12-13 1.1904904528470297 1102 14-15 1.5907363993721475 1102 16-17 1.605781170130065 1102 18-19 1.3586827186614272 1102 20-21 1.5231763320543195 1102 22-23 1.4912276071194128 1102 24-25 1.5751521457235 1102 26-27 1.3360165828321229 1102 28-29 1.5652591921786652 1102 30-31 1.683603573814331 1102 32-33 1.639624369162462 1102 34-35 1.6281059933297541 1102 36-37 1.668259682329463 1102 38-39 1.5582022791843428 1102 40-41 1.4581072970251725 1102 42-43 1.523716116160628 1102 44-45 1.4481796780483052 1102 46-47 1.7553542285867394 1102 48-49 1.72228446762103 1102 50-51 1.8382779448530329 1102 52-53 1.7471302736800745 1102 54-55 1.708871878631733 1102 56-57 1.7696934984792314 1102 58-59 1.8015084716466525 1102 60-61 1.5986193755329374 1102 62-63 1.317589384213985 1102 64-65 1.330942990789616 1102 66-67 1.2851755250172765 1102 68-69 1.2106694442061965 1102 70-71 1.2157178268151903 1102 72-73 1.2105866264829004 1102 74-75 1.2277357202604584 1102 76-77 0.9713732826323067 1102 78-79 0.9957304287429309 1102 80-81 0.7037959343589009 1102 82-83 0.5407321254974313 1102 84-85 0.2561586640475575 1102 86-87 -0.09813328708838753 1102 88-89 -0.8509336429951126 1102 90-91 -4.6926094982717785 1102 92-93 -10.051945954938688 1102 94-95 -8.67791128391984 1102 96-97 -4.88784511017508 1102 98-99 -3.3223770857844954 1102 100-101 -2.7672884994603812 1103 1 0.797669512702452 1103 2 0.8109509736511633 1103 3 0.938063009159702 1103 4 0.8119674497336646 1103 5 0.8582453784585269 1103 6 0.6943856568206925 1103 7 0.8542074929554886 1103 8 0.7863002847378837 1103 9 0.7292760028561389 1103 10-11 0.7367631011281901 1103 12-13 0.8495363284828841 1103 14-15 0.9124927989455145 1103 16-17 0.9444468674931485 1103 18-19 0.9828425239619918 1103 20-21 1.0256420245602769 1103 22-23 1.1410867303110734 1103 24-25 0.8353167946223365 1103 26-27 0.9580018251055904 1103 28-29 0.9804796005840615 1103 30-31 1.118410576369108 1103 32-33 1.2345249153512299 1103 34-35 1.163570341364938 1103 36-37 1.2715462504812294 1103 38-39 1.1381492209612318 1103 40-41 1.0573587608341413 1103 42-43 1.1042311834299525 1103 44-45 1.0724025862854916 1103 46-47 1.1176264956622575 1103 48-49 1.2118701938562069 1103 50-51 1.1418183978166638 1103 52-53 1.1327097198791485 1103 54-55 1.1095470601472286 1103 56-57 0.873971997019499 1103 58-59 0.9590755090613179 1103 60-61 0.7421191360602819 1103 62-63 0.8800286451492028 1103 64-65 0.6347660135277771 1103 66-67 0.6504460773650429 1103 68-69 0.6969737182059141 1103 70-71 0.7733864460234052 1103 72-73 0.6873880361666735 1103 74-75 0.6840207130285059 1103 76-77 0.21198109728405257 1103 78-79 0.39639267117047794 1103 80-81 0.20252362388767509 1103 82-83 0.17708702119046293 1103 84-85 -0.018724794582592352 1103 86-87 -0.513382858207386 1103 88-89 -1.0515582824424499 1103 90-91 -2.3998189391080977 1103 92-93 -9.016246812222938 1103 94-95 -5.496614954520123 1103 96-97 -2.971176718296686 1103 98-99 -2.0394747766947656 1103 100-101 -1.4972803896381794 1104 1 0.8963014588702762 1104 2 0.9155277926122309 1104 3 0.8452267505986697 1104 4 0.8302288005014162 1104 5 0.7751406721365512 1104 6 0.827458843136732 1104 7 0.9519511739787276 1104 8 0.8435106643756782 1104 9 1.0329582811963363 1104 10-11 0.7103412065618926 1104 12-13 0.825113600989738 1104 14-15 0.8460971187552033 1104 16-17 1.0270272862956205 1104 18-19 0.8597278054028408 1104 20-21 0.8954444326316846 1104 22-23 0.7443626715612055 1104 24-25 0.7651041986004188 1104 26-27 0.7705469300914558 1104 28-29 0.7778872583766301 1104 30-31 0.6906542873434844 1104 32-33 0.8123295540692084 1104 34-35 0.5821882532857785 1104 36-37 0.6386012835558503 1104 38-39 0.5664399238431059 1104 40-41 0.5756077032737608 1104 42-43 0.4936505877731534 1104 44-45 0.4397327419458321 1104 46-47 0.5129609642863002 1104 48-49 0.5148824224775126 1104 50-51 0.30859017871125616 1104 52-53 0.5481947428844656 1104 54-55 0.6271078395613898 1104 56-57 0.5981546875443833 1104 58-59 0.4019427171304386 1104 60-61 0.4248983733617102 1104 62-63 0.21220614984867936 1104 64-65 0.28325390898974945 1104 66-67 0.3424569681689036 1104 68-69 0.3388331418319446 1104 70-71 0.3288127362755944 1104 72-73 0.29050081503315184 1104 74-75 0.33433505734106816 1104 76-77 0.1532935561012394 1104 78-79 -0.09129555746223517 1104 80-81 -0.20877094982207112 1104 82-83 -0.22046682667487616 1104 84-85 -0.15623260327184596 1104 86-87 -0.19622656176529674 1104 88-89 -0.2654721543827989 1104 90-91 -1.028571602619909 1104 92-93 -4.515011399139711 1104 94-95 -1.9173728038573472 1104 96-97 -0.9918501339528589 1104 98-99 -0.8973932867825951 1104 100-101 -0.67306261349583 1105 1 0.9972868899644958 1105 2 1.005656901940668 1105 3 0.9753240608564191 1105 4 0.9428782837665182 1105 5 0.8343991756246751 1105 6 0.8324191580665641 1105 7 0.9442875129194164 1105 8 0.889882466932356 1105 9 1.104264296237126 1105 10-11 1.07224800297557 1105 12-13 0.9793169875057401 1105 14-15 1.1761678589338374 1105 16-17 1.2866314133728736 1105 18-19 1.0472951339833187 1105 20-21 0.9710065492254429 1105 22-23 0.9829519916647627 1105 24-25 1.1196532087055502 1105 26-27 1.034128491731913 1105 28-29 0.9475223342820343 1105 30-31 1.180191627869867 1105 32-33 1.3389693530718674 1105 34-35 1.147774474255307 1105 36-37 1.1694079086456526 1105 38-39 1.2209933977215286 1105 40-41 0.9502475192812767 1105 42-43 1.009514838050542 1105 44-45 1.0383083731446305 1105 46-47 1.0273987201561638 1105 48-49 0.8796179757940976 1105 50-51 0.8645703595578453 1105 52-53 0.7748077592473841 1105 54-55 0.9409893242549003 1105 56-57 0.8930287878815086 1105 58-59 0.9541718404878772 1105 60-61 0.9409320344635397 1105 62-63 0.9238969846593115 1105 64-65 1.1311833558644686 1105 66-67 0.9364835892625685 1105 68-69 0.7694550517317431 1105 70-71 0.6973218785084185 1105 72-73 0.9402755007622545 1105 74-75 0.8421766316501405 1105 76-77 0.4552882250805652 1105 78-79 0.422252844719484 1105 80-81 0.558797824170707 1105 82-83 0.6939199146430859 1105 84-85 0.3751115876586191 1105 86-87 0.1280002489324552 1105 88-89 -0.2953975030885161 1105 90-91 -1.3633210148897597 1105 92-93 -7.433858725339508 1105 94-95 -3.174522280745631 1105 96-97 -1.3943560112543594 1105 98-99 -0.5077135306321523 1105 100-101 -0.20354784690865912 1106 1 0.9392385810360331 1106 2 0.96929175985235 1106 3 0.8932829925369106 1106 4 0.8347542640039833 1106 5 0.761217334607224 1106 6 0.7089653624771799 1106 7 0.8098493665906616 1106 8 0.6635317997821417 1106 9 0.9430100318607018 1106 10-11 0.7827649403801047 1106 12-13 0.7382299855919712 1106 14-15 0.7874335446958014 1106 16-17 0.8944913871861075 1106 18-19 0.8651625964575942 1106 20-21 0.8679897727407777 1106 22-23 0.925858506642868 1106 24-25 0.9515513134620264 1106 26-27 0.8586901224702785 1106 28-29 0.6877509660863907 1106 30-31 0.8276628114394029 1106 32-33 0.8946249602264444 1106 34-35 1.0266236970516047 1106 36-37 1.0923829283877708 1106 38-39 1.1184367146802856 1106 40-41 1.112210419795126 1106 42-43 1.290100111449469 1106 44-45 1.2157386163455612 1106 46-47 1.2219852777741096 1106 48-49 1.1516597780651878 1106 50-51 1.0045247211141657 1106 52-53 0.8988967958162313 1106 54-55 1.3218570120169773 1106 56-57 1.1530257382538203 1106 58-59 1.2016266573296726 1106 60-61 1.1719056153465708 1106 62-63 1.212545520759523 1106 64-65 1.3059991418677406 1106 66-67 1.1169915230044047 1106 68-69 0.9696098291677906 1106 70-71 0.801928033026023 1106 72-73 1.0379849634783014 1106 74-75 0.9863378702150172 1106 76-77 0.7945752009331635 1106 78-79 0.7283669753675746 1106 80-81 0.7336704227360862 1106 82-83 0.825806892379628 1106 84-85 0.6797475123512591 1106 86-87 0.8235241647643008 1106 88-89 0.5612379098004574 1106 90-91 0.02424496225490813 1106 92-93 -3.4926989758721145 1106 94-95 -1.2109966324740675 1106 96-97 -0.06375713816439799 1106 98-99 0.39893773875084193 1106 100-101 0.5616985253296036 1107 1 0.6723388714174305 1107 2 0.6265978158426861 1107 3 0.6233061405113673 1107 4 0.704013603855266 1107 5 0.5074838326976092 1107 6 0.5461319303491408 1107 7 0.5182927497215317 1107 8 0.469412961007599 1107 9 0.6023123661669771 1107 10-11 0.5810549165943399 1107 12-13 0.5665755619652799 1107 14-15 0.6400250298428318 1107 16-17 0.6112319310322007 1107 18-19 0.470405687706382 1107 20-21 0.5612804242605947 1107 22-23 0.5704332704735009 1107 24-25 0.46029623929097596 1107 26-27 0.36870678000781965 1107 28-29 0.383133751796926 1107 30-31 0.5034533879252692 1107 32-33 0.5392687552937119 1107 34-35 0.4146279252148517 1107 36-37 0.4884428017304643 1107 38-39 0.3298106426326797 1107 40-41 0.46591728465146787 1107 42-43 0.36679672291615617 1107 44-45 0.08115846882881783 1107 46-47 0.28134079239447374 1107 48-49 0.30867216992249524 1107 50-51 0.5133415857454082 1107 52-53 0.5833011762983951 1107 54-55 0.5559413726901852 1107 56-57 0.332285509550335 1107 58-59 0.33847142333629066 1107 60-61 0.4406466581684505 1107 62-63 0.4269219323357625 1107 64-65 0.589203072896531 1107 66-67 0.28365750185983885 1107 68-69 0.26161524286749227 1107 70-71 0.149285337206166 1107 72-73 0.16234895693533957 1107 74-75 0.3081425493323202 1107 76-77 0.16904827499918085 1107 78-79 0.11150665857422837 1107 80-81 0.12262673796873003 1107 82-83 0.28216133026277035 1107 84-85 -0.08258122265133139 1107 86-87 -0.25727389635835607 1107 88-89 -0.3462061148707072 1107 90-91 -0.453036726459235 1107 92-93 -3.7777830701747863 1107 94-95 -1.5056471805970908 1107 96-97 -0.729688347036415 1107 98-99 -0.49171706379731006 1107 100-101 -0.32356311806403326 1108 1 0.4526556517495095 1108 2 0.35438696837590555 1108 3 0.0010884467632124029 1108 4 -0.17382327979458978 1108 5 -0.7749862799092568 1108 6 -0.048220819638160606 1108 7 0.19579882619802902 1108 8 0.2784598236429119 1108 9 0.4790217026305257 1108 10-11 0.3788009793397009 1108 12-13 0.3406892256643701 1108 14-15 0.5402300968624658 1108 16-17 0.474959239466493 1108 18-19 0.49682429827768715 1108 20-21 0.4215122078486715 1108 22-23 0.43687369914714225 1108 24-25 0.25426900459080315 1108 26-27 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70-71 0.6516769231790605 2311 72-73 0.6446055631302912 2311 74-75 0.7797696954949878 2311 76-77 0.8676926439349941 2311 78-79 0.8052113290908629 2311 80-81 0.8993422705476242 2311 82-83 1.022631140583794 2311 84-85 0.8812168047262219 2311 86-87 1.097997266312511 2311 88-89 1.2760434913825733 2311 90-91 2.1052491506723463 2311 92-93 3.0865184425903855 2311 94-95 2.386722882985712 2311 96-97 1.9706815804364126 2311 98-99 1.9787937375015687 2311 100-101 2.144417678276554 2312 1 0.2873746984200345 2312 2 0.0886197386716141 2312 3 0.16182865237693989 2312 4 0.025814747022685935 2312 5 -0.05409364622554591 2312 6 0.034010137932448004 2312 7 0.2093993871706843 2312 8 -0.4639197259206327 2312 9 0.1817021435935402 2312 10-11 0.15703003888610567 2312 12-13 0.20774443610536508 2312 14-15 -0.04352242517949634 2312 16-17 0.31708060436653085 2312 18-19 0.33033646984038967 2312 20-21 0.3026141317008708 2312 22-23 0.17056471291031983 2312 24-25 0.2164753064314553 2312 26-27 0.1816671618393073 2312 28-29 0.17116388477861477 2312 30-31 0.24535940627993824 2312 32-33 0.19142643531348824 2312 34-35 -0.07536978408707284 2312 36-37 0.18406623520809973 2312 38-39 0.3071715160552486 2312 40-41 0.45888229635948363 2312 42-43 0.2995564929252268 2312 44-45 0.19869891358784741 2312 46-47 0.4484698205082438 2312 48-49 0.27904108139247086 2312 50-51 0.16914196049877006 2312 52-53 0.0033494506391846812 2312 54-55 0.04792786847966113 2312 56-57 0.0192945244774414 2312 58-59 0.04606254221503647 2312 60-61 0.29812512075856645 2312 62-63 0.16455513218643958 2312 64-65 0.4136674252713384 2312 66-67 0.4165511383248415 2312 68-69 0.22998118733806194 2312 70-71 0.3585891467946176 2312 72-73 0.46098647638809354 2312 74-75 0.6255457340086394 2312 76-77 0.6612249956891532 2312 78-79 0.698833947211881 2312 80-81 0.7996398252479615 2312 82-83 0.8010754060569987 2312 84-85 0.8308577211550592 2312 86-87 0.7491614502036583 2312 88-89 1.181307047553119 2312 90-91 1.6298809683955433 2312 92-93 2.7676223227829553 2312 94-95 1.970043604251817 2312 96-97 1.843083494587873 2312 98-99 1.9237762968143173 2312 100-101 1.7547593031432243 2313 1 -0.2103297363955221 2313 2 -0.8520883737170308 2313 3 -0.16677632994449 2313 4 0.007705213524985766 2313 5 -0.8521321691627932 2313 6 -0.36496748597465256 2313 7 -1.187903980286741 2313 8 -0.9327932357224071 2313 9 -1.112596630488909 2313 10-11 -1.9841368947398053 2313 12-13 -0.4897981845964239 2313 14-15 0.01706011460850476 2313 16-17 -1.4881453921965218 2313 18-19 -0.9868026952355962 2313 20-21 -1.4415984717084243 2313 22-23 -1.2414344691528925 2313 24-25 -0.273496662847883 2313 26-27 -0.8687780346001972 2313 28-29 -2.2708733748907193 2313 30-31 -0.5929495969505112 2313 32-33 -0.262386285893065 2313 34-35 -0.0035616005891085933 2313 36-37 -0.12033329503939427 2313 38-39 -0.39906213194282714 2313 40-41 -0.16905477907207 2313 42-43 0.02088718001008516 2313 44-45 -0.538358650846007 2313 46-47 -0.6338691869061535 2313 48-49 0.0376583731687532 2313 50-51 0.005279974537472754 2313 52-53 0.19290125090818222 2313 54-55 0.2642038004876568 2313 56-57 -0.13958823761698014 2313 58-59 0.0637454871106975 2313 60-61 -0.029836331164528218 2313 62-63 -0.08784402186400797 2313 64-65 0.13976331242299977 2313 66-67 0.17247972104060594 2313 68-69 0.2181123499216291 2313 70-71 0.17437705515696678 2313 72-73 0.19399464767536756 2313 74-75 0.18361082866843859 2313 76-77 0.2870247511161743 2313 78-79 0.24881058079595064 2313 80-81 0.38129467477812184 2313 82-83 0.389542430006113 2313 84-85 0.4800231068962013 2313 86-87 0.5406772415013847 2313 88-89 0.6794286435982926 2313 90-91 1.1168873556648187 2313 92-93 2.4014104295690473 2313 94-95 1.7183241188606537 2313 96-97 1.157154835555282 2313 98-99 1.0853861508426803 2313 100-101 0.9267600294998246 2314 1 -0.46308734499200455 2314 2 -0.6573628216946368 2314 3 -1.6351109501989214 2314 4 -0.6379646122177363 2314 5 -0.42826619884282735 2314 6 -2.9086853453983643 2314 7 -3.788116266637445 2314 8 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-0.6926550303450334 2314 72-73 -0.6307373797482896 2314 74-75 -0.3709755801096257 2314 76-77 -0.5664178926277152 2314 78-79 -0.6563178925002511 2314 80-81 -0.5752179989840407 2314 82-83 -0.533106342894218 2314 84-85 -0.5914672144928694 2314 86-87 -0.21925715890093755 2314 88-89 0.16090309914165601 2314 90-91 0.6704503840163945 2314 92-93 1.5783820334881113 2314 94-95 0.956340287336797 2314 96-97 0.8846520373363518 2314 98-99 0.7464910189647895 2314 100-101 0.9310796339440408 2315 1 -7.249372883844895 2315 2 -2.997172915660844 2315 3 -1.3322086333891683 2315 4 -0.769474540619214 2315 5 -1.279403771071813 2315 6 -1.668295226652269 2315 7 -2.984000173965054 2315 8 -2.826063259308828 2315 9 -3.429139838728016 2315 10-11 -2.1240137457872663 2315 12-13 -2.6961605488971543 2315 14-15 -4.499566099251993 2315 16-17 -4.292758006840433 2315 18-19 -3.23549969487528 2315 20-21 -4.446406718775592 2315 22-23 -5.769131599214191 2315 24-25 -3.71827548768281 2315 26-27 -4.571790000230791 2315 28-29 -3.0805638114494514 2315 30-31 -2.8460220207367612 2315 32-33 -2.0468409135393557 2315 34-35 -2.819877234161847 2315 36-37 -3.1938383649033817 2315 38-39 -2.3460345820344557 2315 40-41 -3.6384650523164055 2315 42-43 -7.802737590370505 2315 44-45 -4.469168878553301 2315 46-47 -3.072282110582986 2315 48-49 -5.924979260319979 2315 50-51 -6.9254829477025055 2315 52-53 -7.061324298907351 2315 54-55 -4.6676171947397975 2315 56-57 -3.891514166981519 2315 58-59 -4.239274325922178 2315 60-61 -3.2737351416751856 2315 62-63 -3.4129264195880396 2315 64-65 -2.297285268523506 2315 66-67 -2.789335593109694 2315 68-69 -2.2309521513041126 2315 70-71 -2.051911853037268 2315 72-73 -2.441147125041752 2315 74-75 -3.0337629884185624 2315 76-77 -2.8043936171104633 2315 78-79 -2.0834118477429477 2315 80-81 -1.8228381430582523 2315 82-83 -2.2897700491319526 2315 84-85 -2.1444292881088707 2315 86-87 -2.6124816142368346 2315 88-89 -2.170014743473171 2315 90-91 -0.9976356438554923 2315 92-93 -0.17138771092819383 2315 94-95 -0.2459929692446714 2315 96-97 -0.48190279090394483 2315 98-99 -0.48264563224185864 2315 100-101 -0.36729816064604925 2316 1 -4.194096906808987 2316 2 -2.82747527173915 2316 3 -4.28980900016742 2316 4 -4.456049714990584 2316 5 -3.9186965767568758 2316 6 -4.324124405075732 2316 7 -2.802117603685552 2316 8 -1.4060977796147895 2316 9 -1.5049715792054315 2316 10-11 -2.9136799034142697 2316 12-13 -3.8402592284280317 2316 14-15 -2.8988637256438494 2316 16-17 -2.90559103171595 2316 18-19 -3.098426774100048 2316 20-21 -5.712744195219869 2316 22-23 -6.290826296773833 2316 24-25 -3.9613173199559277 2316 26-27 -3.7071514015394555 2316 28-29 -4.160229867942739 2316 30-31 -5.948807748747761 2316 32-33 -4.263291828293752 2316 34-35 -5.983473987095564 2316 36-37 -9.235402802245993 2316 38-39 -5.744370426854889 2316 40-41 -5.255035810515821 2316 42-43 -4.1242588436194225 2316 44-45 -3.1613397810368085 2316 46-47 -5.296487452465488 2316 48-49 -2.772094084161914 2316 50-51 -4.0495814249659645 2316 52-53 -5.147413020139023 2316 54-55 -6.505885010285397 2316 56-57 -4.66151504347366 2316 58-59 -5.225500251885563 2316 60-61 -5.753668191159907 2316 62-63 -5.918062832105694 2316 64-65 -4.669963659958157 2316 66-67 -6.153423181643831 2316 68-69 -4.6933765959957725 2316 70-71 -5.662573031514668 2316 72-73 -7.506043277578357 2316 74-75 -7.430033345791379 2316 76-77 -6.793148391464303 2316 78-79 -5.761894808398569 2316 80-81 -5.682758011449582 2316 82-83 -4.634341528751822 2316 84-85 -6.0808155110008215 2316 86-87 -4.768443109262741 2316 88-89 -4.600593026020526 2316 90-91 -5.191526999283171 2316 92-93 -4.089985862878219 2316 94-95 -4.031094288365349 2316 96-97 -4.896940362076794 2316 98-99 -5.7587484110590665 2316 100-101 -4.783532480707269 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 12288.0 3 5120.0 4 5103.0 5 5919.0 6 6848.0 7 8138.0 8 10040.0 9 12621.0 10 15669.0 11 18910.0 12 22567.0 13 26437.0 14 28698.0 15 32556.0 16 36788.0 17 40735.0 18 36982.0 19 34185.0 20 36397.0 21 39446.0 22 43850.0 23 48930.0 24 54636.0 25 61886.0 26 69426.0 27 78566.0 28 90018.0 29 101547.0 30 115255.0 31 132920.0 32 152673.0 33 177434.0 34 208731.0 35 249502.0 36 287321.0 37 255129.0 38 81298.0 39 1344.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.34330594043975 18.65157964341322 13.316396243173841 21.688718172973186 2 14.727029839955419 21.696533861783497 40.71075184974582 22.865684448515267 3 20.434965845568094 27.158485946291133 29.85978069737388 22.546767510766895 4 11.939581935870502 16.31740972910313 39.253014263484594 32.48999407154177 5 12.552197575626428 39.61193528446402 33.9344960621521 13.901371077757457 6 29.223717521249036 37.17835195007729 18.756796646603124 14.841133882070546 7 27.224948476072015 34.613676463022315 21.19320640742792 16.968168653477754 8 25.291249593818556 33.706156519160224 20.64320983778087 20.35938404924035 9 27.25363553662582 17.479172143533955 20.91111115313983 34.3560811667004 10-11 24.402296054724605 26.48872267770157 28.00548592751025 21.103495340063574 12-13 27.380010274187654 24.2476692044465 27.667884236082372 20.704436285283474 14-15 23.817646583715472 25.26968029155301 27.760107726468743 23.15256539826278 16-17 21.294818316990025 28.75047310574701 28.279154804739964 21.675553772523003 18-19 20.91172906736961 28.504093065942644 30.22293864694843 20.361239219739325 20-21 22.807701882717755 26.794033810375268 29.05201252774211 21.346251779164874 22-23 21.861815388719304 26.896384717036636 28.82676173980822 22.415038154435837 24-25 22.29027516213614 26.76237896559868 29.55638886029345 21.390957011971736 26-27 22.536002363286396 26.168476507271627 30.205214937378024 21.090306192063952 28-29 21.299419343471875 27.167110348585055 30.37441303692467 21.1590572710184 30-31 21.503334717358054 27.47338906524585 29.73496368165211 21.288312535743984 32-33 21.48491580783977 27.350567904411903 29.38234481505732 21.782171472691008 34-35 22.33528213613412 26.612795792765866 29.29091104512498 21.761011025975037 36-37 22.564319459386322 26.344187049910445 29.935233467029626 21.15626002367361 38-39 22.605093997485984 26.370159225502572 30.158158284685673 20.86658849232578 40-41 21.801482421107423 26.770088872229593 30.349197531202915 21.07923117546007 42-43 21.178689295716975 27.58696962690298 29.325264720607695 21.909076356772346 44-45 21.670091456163988 27.48984340693178 28.690660815671908 22.14940432123233 46-47 22.031242933287967 27.282262952555786 28.017740321194655 22.66875379296159 48-49 22.334782501717616 27.15873409432023 28.333639317950826 22.172844086011327 50-51 22.327871589359734 27.654666704776403 28.632238302975832 21.385223402888023 52-53 22.036812956319068 27.948686300849147 28.457745668329014 21.556755074502775 54-55 21.135157860942442 27.189028982991527 29.0384932456184 22.63731991044763 56-57 22.08972961516335 28.135086444424257 27.662859175825837 22.11232476458655 58-59 22.332129119569117 27.31546237721334 27.979520797097173 22.372887706120373 60-61 22.023115284273114 26.982521057758312 28.892988971781964 22.10137468618661 62-63 22.737574326234316 26.8073389079507 28.490641507349086 21.964445258465897 64-65 22.14342779772705 27.41196543942222 28.626288733418882 21.818318029431847 66-67 21.553135700941088 27.424398779527454 28.399940512748323 22.622525006783135 68-69 21.37321994999352 28.02542516387387 28.337232501752496 22.264122384380116 70-71 21.80696587678586 27.794422848112188 27.637691755751536 22.760919519350413 72-73 21.763830873352617 27.083472529610713 27.837015717617774 23.315680879418892 74-75 21.532520964673115 27.981362126855892 27.804724853040476 22.68139205543052 76-77 22.20204376455684 26.79488201281598 28.08270849633806 22.92036572628912 78-79 21.65775385884604 27.086896610735163 28.475261492107958 22.780088038310844 80-81 21.677431226841808 27.97787945358593 27.879434654235357 22.465254665336904 82-83 21.620152112417944 27.602020088047546 27.755196467496358 23.022631332038152 84-85 21.59005737179924 27.144496228966325 27.68348300877157 23.581963390462864 86-87 21.892904150441144 27.152132116376336 27.98346198296457 22.97150175021795 88-89 22.192614400911104 26.56516333143268 27.963148485116957 23.27907378253926 90-91 21.327933648601256 26.77989987947719 28.599640875744786 23.29252559617677 92-93 20.99103083300754 27.390495578106066 28.677740303349104 22.94073328553729 94-95 21.268369922359227 27.437093404434 27.655503044520962 23.639033628685816 96-97 20.970691029179463 26.971270025108122 27.66273278883481 24.395306156877602 98-99 21.427680015240867 26.967853092269557 27.198056865028363 24.406410027461213 100-101 21.683244447137966 26.79721826611315 27.609977727947637 23.90955955880125 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3202.0 1 2003.5 2 841.0 3 877.0 4 789.5 5 750.5 6 1084.5 7 1905.0 8 2954.0 9 3406.0 10 2991.0 11 2558.5 12 2597.5 13 2810.0 14 3083.5 15 3329.5 16 3544.0 17 3858.0 18 4292.0 19 4731.0 20 5086.5 21 5734.0 22 6606.5 23 7775.5 24 9394.0 25 11632.0 26 15114.0 27 19396.5 28 23413.5 29 27779.0 30 32714.0 31 37708.5 32 43000.0 33 48831.5 34 55863.5 35 61238.0 36 66201.5 37 71463.0 38 73995.0 39 74979.0 40 76056.0 41 77870.0 42 79630.0 43 82385.5 44 86184.5 45 89170.0 46 94856.5 47 112138.0 48 156815.5 49 159389.0 50 116519.5 51 96009.5 52 85334.0 53 79955.5 54 74818.5 55 67764.5 56 60852.5 57 54110.0 58 47703.5 59 41839.0 60 35950.5 61 29635.0 62 23795.0 63 18524.5 64 13864.5 65 10375.0 66 7475.5 67 5418.0 68 3845.0 69 2576.0 70 1858.0 71 1389.0 72 1060.0 73 822.0 74 598.5 75 426.0 76 314.0 77 211.5 78 133.0 79 86.5 80 50.5 81 38.0 82 28.0 83 20.5 84 14.5 85 12.5 86 13.0 87 10.0 88 9.0 89 6.0 90 3.5 91 3.5 92 3.0 93 2.5 94 3.0 95 2.0 96 0.5 97 0.5 98 0.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.09089490092833741 2 0.10249770117158048 3 0.09920961120036827 4 0.103177995648383 5 0.0979246105219635 6 0.08110621928990107 7 0.09376725538594806 8 0.0903279888643353 9 0.08382739719711117 10-11 0.09153740126753979 12-13 0.09051695955233599 14-15 0.09643174208675795 16-17 0.09359718176674744 18-19 0.09599710950435635 20-21 0.10654167389479549 22-23 0.11472410468522587 24-25 0.13942257360691754 26-27 0.14473264993973725 28-29 0.14427912028853557 30-31 0.14588537113654154 32-33 0.1414634570373251 34-35 0.13632345432370604 36-37 0.14099103031732335 38-39 0.13335661452209502 40-41 0.1289347004228786 42-43 0.1288780092164784 44-45 0.11986410739884493 46-47 0.13921470585011678 48-49 0.15752596551738474 50-51 0.16699339698621987 52-53 0.176460828455055 54-55 0.17209560556223882 56-57 0.14184139841332652 58-59 0.13322433504049455 60-61 0.12921815645487966 62-63 0.12623241958446857 64-65 0.11185175022761519 66-67 0.12585447820846718 68-69 0.12303881495725673 70-71 0.13186374608688947 72-73 0.16098412910779758 74-75 0.17477898933184877 76-77 0.17980560963266745 78-79 0.1767442844870561 80-81 0.18288583184707885 82-83 0.182923625984679 84-85 0.15862199550778883 86-87 0.1603227316997951 88-89 0.17835053533506204 90-91 0.26431330130658115 92-93 0.5251873360915496 94-95 0.1804670070406699 96-97 0.19405399950792032 98-99 0.21329121554639172 100-101 0.22045320462161833 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2645913.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.8164191056553 #Duplication Level Percentage of deduplicated Percentage of total 1 73.4685760649454 35.8647280028209 2 13.514109439641784 13.194208604904917 3 5.283145463327128 7.737127294017555 4 2.6044834159062833 5.085662159384395 5 1.4100609723104136 3.441706369441648 6 0.8859270601230212 2.594867199840389 7 0.5914152119366809 2.0209540995952677 8 0.4167693609057095 1.6276150233895381 9 0.2904007681277916 1.2758693044977452 >10 1.389964605772076 11.905256379825522 >50 0.08776024217064922 2.9433093169948625 >100 0.05056054764705623 4.485365316058205 >500 0.0027783680408572375 0.8901201745770925 >1k 0.0031752777609772333 3.1440966636598504 >5k 5.556736081710158E-4 1.6722401346757072 >10k+ 3.175277760977233E-4 2.1168739563165913 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 17747 0.6707325599896898 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 13560 0.5124885058579024 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11775 0.44502597024165197 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 11545 0.4363333185936197 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7936 0.2999342759947134 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 6496 0.24551071785051132 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 6135 0.2318670341768607 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 6086 0.2300151214344538 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5590 0.21126922918478422 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 5497 0.2077543743879712 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 5413 0.20457966682955941 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 4779 0.18061818359107046 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 4474 0.16909097162302766 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 4452 0.1682595005958246 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 4363 0.16489582234941208 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 3507 0.132544040563692 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 3194 0.1207144754948481 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 2871 0.10850696905000277 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 2660 0.10053240601637317 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 7.558827520028058E-5 8 0.0 0.0 0.0 0.0 7.558827520028058E-5 9 0.0 0.0 0.0 0.0 1.1338241280042087E-4 10-11 0.0 0.0 0.0 3.779413760014029E-5 1.1338241280042087E-4 12-13 0.0 0.0 0.0 3.779413760014029E-5 1.1338241280042087E-4 14-15 0.0 0.0 0.0 5.669120640021044E-5 1.1338241280042087E-4 16-17 0.0 0.0 0.0 9.448534400035072E-5 1.8897068800070147E-4 18-19 0.0 0.0 0.0 1.1338241280042087E-4 2.2676482560084175E-4 20-21 1.8897068800070146E-5 0.0 0.0 1.8897068800070144E-4 2.456618944009119E-4 22-23 3.779413760014029E-5 0.0 0.0 4.913237888018238E-4 3.212501696011925E-4 24-25 3.779413760014029E-5 0.0 0.0 0.0011905153344044191 3.7794137600140294E-4 26-27 3.779413760014029E-5 0.0 0.0 0.0019086039488070848 3.7794137600140294E-4 28-29 3.779413760014029E-5 0.0 0.0 0.005442355814420203 3.7794137600140294E-4 30-31 3.779413760014029E-5 0.0 0.0 0.015514493484857589 3.7794137600140294E-4 32-33 3.779413760014029E-5 0.0 0.0 0.03635796037133496 3.7794137600140294E-4 34-35 3.779413760014029E-5 0.0 0.0 0.06767040337305119 4.157355136015432E-4 36-37 5.669120640021044E-5 0.0 0.0 0.11512094313002733 4.535296512016835E-4 38-39 7.558827520028058E-5 0.0 0.0 0.18056149238467023 4.913237888018238E-4 40-41 7.558827520028058E-5 0.0 0.0 0.25505373759454675 4.913237888018238E-4 42-43 7.558827520028058E-5 0.0 0.0 0.33005620366202515 5.29117926401964E-4 44-45 7.558827520028058E-5 0.0 0.0 0.4187023534031542 5.669120640021043E-4 46-47 7.558827520028058E-5 0.0 0.0 0.5259999100499525 6.047062016022447E-4 48-49 7.558827520028058E-5 0.0 0.0 0.6465443119256 6.802944768025252E-4 50-51 7.558827520028058E-5 0.0 0.0 0.7719452604828655 6.802944768025252E-4 52-53 7.558827520028058E-5 0.0 0.0 0.8922628975329121 7.180886144026655E-4 54-55 7.558827520028058E-5 0.0 0.0 1.02100862726779 7.936768896029461E-4 56-57 7.558827520028058E-5 0.0 0.0 1.1725631190443526 8.314710272030864E-4 58-59 7.558827520028058E-5 0.0 0.0 1.3517640224754177 8.314710272030864E-4 60-61 7.558827520028058E-5 0.0 0.0 1.573596713119441 8.314710272030864E-4 62-63 7.558827520028058E-5 0.0 0.0 1.778535424256202 8.314710272030864E-4 64-65 7.558827520028058E-5 0.0 0.0 1.9706808198153154 8.314710272030864E-4 66-67 7.558827520028058E-5 0.0 0.0 2.193930790619344 8.503680960031566E-4 68-69 7.558827520028058E-5 0.0 0.0 2.4489656311450907 8.692651648032268E-4 70-71 7.558827520028058E-5 3.779413760014029E-5 0.0 2.7337633550309475 8.881622336032969E-4 72-73 7.558827520028058E-5 3.779413760014029E-5 0.0 3.034963734635266 9.826475776036475E-4 74-75 7.558827520028058E-5 3.779413760014029E-5 0.0 3.3457071339836193 9.826475776036475E-4 76-77 7.558827520028058E-5 7.558827520028058E-5 0.0 3.659266196583183 9.826475776036475E-4 78-79 7.558827520028058E-5 7.558827520028058E-5 0.0 4.0206537403157245 9.826475776036475E-4 80-81 9.448534400035072E-5 7.558827520028058E-5 0.0 4.419854318717206 0.0010015446464037177 82-83 1.1338241280042087E-4 7.558827520028058E-5 0.0 4.8153510716338745 0.0010582358528039282 84-85 1.1338241280042087E-4 7.558827520028058E-5 0.0 5.241347693593855 0.0010582358528039282 86-87 1.1338241280042087E-4 7.558827520028058E-5 0.0 5.6987890380371535 0.0010582358528039282 88-89 1.1338241280042087E-4 7.558827520028058E-5 0.0 6.153754866467643 0.0011338241280042087 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 16765 0.0 28.561638 1 TACATGG 16585 0.0 28.38572 2 ACATGGG 16935 0.0 26.509995 3 CATGGGA 12305 0.0 24.103352 4 CATGGGG 4675 0.0 23.245178 4 GAGTACT 9570 0.0 21.743864 12-13 ATGGGAT 4650 0.0 21.737305 5 ATGGGAG 4580 0.0 21.447857 5 AGTACTT 9900 0.0 21.330639 12-13 TAGGACG 320 1.8189894E-11 20.761421 4 GTACTTT 10600 0.0 19.721672 14-15 CGTATAC 150 3.4870027E-9 19.101309 90-91 AGTACAT 12010 0.0 17.268003 2 ATGGGGG 2600 0.0 17.15669 5 GCGTATC 195 2.2927162E-4 17.035658 1 ATGGGGA 2515 0.0 16.981792 5 ACTTTTT 13285 0.0 16.897234 16-17 TACTTTT 12715 0.0 16.627808 14-15 TAAAGCG 345 2.1229425E-8 16.505974 5 GCTTATA 2725 0.0 16.196156 1 >>END_MODULE