##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939647_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2645913 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.572171118249162 31.0 31.0 34.0 30.0 34.0 2 31.764031168069398 31.0 31.0 34.0 30.0 34.0 3 31.815114480332497 31.0 31.0 34.0 30.0 34.0 4 35.57534318021794 37.0 35.0 37.0 33.0 37.0 5 35.324262362367925 37.0 35.0 37.0 33.0 37.0 6 35.36150357173497 37.0 35.0 37.0 33.0 37.0 7 35.29666621691643 37.0 35.0 37.0 32.0 37.0 8 35.271253816735474 37.0 35.0 37.0 32.0 37.0 9 36.860772066201726 39.0 37.0 39.0 33.0 39.0 10-11 36.745856156268175 39.0 35.0 39.0 32.0 39.0 12-13 36.697718330118946 39.0 35.0 39.0 32.0 39.0 14-15 37.68924885285344 39.0 36.5 41.0 32.0 41.0 16-17 37.67627601512219 39.0 36.5 41.0 32.0 41.0 18-19 37.717713507586986 39.5 37.0 41.0 32.0 41.0 20-21 37.66842881833227 39.0 37.0 41.0 32.0 41.0 22-23 37.53438170491623 39.0 36.0 41.0 32.0 41.0 24-25 37.39854976335201 39.0 36.0 41.0 31.5 41.0 26-27 37.09536632534781 39.0 36.0 40.5 31.0 41.0 28-29 36.896730731509315 39.0 36.0 40.0 30.5 41.0 30-31 36.68316569743601 38.5 35.5 40.0 30.0 41.0 32-33 36.52114903248898 38.0 35.0 40.0 30.0 41.0 34-35 36.49649516064965 38.0 35.0 40.0 30.0 41.0 36-37 36.36096840674656 38.0 35.0 40.0 30.0 41.0 38-39 36.35443134373655 38.5 35.0 40.0 30.0 41.0 40-41 36.25582341520678 38.5 35.0 40.0 30.0 41.0 42-43 36.08459121671801 38.0 35.0 40.0 30.0 41.0 44-45 35.86363402727149 38.0 35.0 40.0 29.0 41.0 46-47 35.52654357871933 38.0 35.0 40.0 27.5 41.0 48-49 35.2012244922641 38.0 34.0 40.0 26.5 41.0 50-51 34.94946262405453 38.0 34.0 40.0 26.0 41.0 52-53 34.67536007419746 38.0 34.0 40.0 25.0 41.0 54-55 34.41852585478056 38.0 33.0 40.0 24.0 41.0 56-57 32.49981273004819 37.0 30.5 39.5 15.5 41.0 58-59 27.619127877598395 33.0 17.5 38.5 6.5 40.0 60-61 28.325914155151736 33.0 20.0 38.0 8.0 40.0 62-63 31.326350299499644 35.0 29.5 38.5 9.5 40.0 64-65 31.532968582111355 35.5 31.0 39.0 5.0 40.0 66-67 24.918485037111953 28.0 18.0 35.5 2.0 39.0 68-69 17.503827412314767 13.0 7.5 28.0 2.0 35.5 70-71 16.388193602737505 13.0 7.5 27.0 2.0 34.0 72-73 17.530313543944942 14.0 7.0 29.0 2.0 34.0 74-75 18.51809639999501 17.5 8.5 30.0 2.0 34.5 76-77 19.662603418933273 21.5 8.0 30.0 2.0 34.5 78-79 22.734537756910374 27.5 10.5 33.5 2.0 35.5 80-81 26.32096576871575 31.5 22.5 34.5 2.0 36.0 82-83 27.590499196307665 33.5 26.5 35.0 2.0 36.0 84-85 27.63736184825427 34.0 27.0 35.0 2.0 36.0 86-87 27.398250811723592 34.0 27.0 35.0 2.0 36.0 88-89 26.953712196886293 33.0 26.0 35.0 2.0 35.0 90-91 25.661720925820312 32.5 20.0 35.0 2.0 35.0 92-93 23.33500534598076 31.5 2.0 34.5 2.0 35.0 94-95 16.085830675460606 7.5 2.0 32.0 2.0 35.0 96-97 13.517372642259968 2.0 2.0 30.0 2.0 34.0 98-99 13.222770174227195 2.0 2.0 30.5 2.0 34.0 100-101 12.922357235479776 2.0 2.0 30.5 2.0 34.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.4689045903156419 1101 2 0.49152678659598337 1101 3 0.493046765232112 1101 4 0.331974994674205 1101 5 0.5938752725525802 1101 6 0.5682769098777953 1101 7 0.5117763384543679 1101 8 0.4743615384159696 1101 9 0.6197584278216794 1101 10-11 0.6134979040230633 1101 12-13 0.6616415075236759 1101 14-15 0.7896466724928928 1101 16-17 0.6917574068538954 1101 18-19 0.7067185201765795 1101 20-21 0.6297065367860739 1101 22-23 0.7202816217670787 1101 24-25 0.6574560893750743 1101 26-27 0.5010287872956667 1101 28-29 0.5869414946589231 1101 30-31 0.5819184933570654 1101 32-33 0.6432121744807802 1101 34-35 0.4851436385929446 1101 36-37 0.4600831024842975 1101 38-39 0.6159767254193014 1101 40-41 0.4380558739857676 1101 42-43 0.5799629166895883 1101 44-45 0.3363731337932947 1101 46-47 0.5802344816611793 1101 48-49 0.46276248975771495 1101 50-51 0.11515106406953635 1101 52-53 0.22671075085602155 1101 54-55 0.3892504248111095 1101 56-57 -2.199707236427251 1101 58-59 -10.10159897705708 1101 60-61 -9.729208687759723 1101 62-63 -3.438509934555615 1101 64-65 -4.664171171686444 1101 66-67 -9.239020898750688 1101 68-69 -4.435242539390547 1101 70-71 -4.328636642214331 1101 72-73 -4.464586551823913 1101 74-75 -6.346772952756204 1101 76-77 -7.576088924045795 1101 78-79 -11.10696978381386 1101 80-81 -7.711148590553442 1101 82-83 -3.5506518899715083 1101 84-85 -2.7422338898205574 1101 86-87 -2.614718772014186 1101 88-89 -2.6188718501171273 1101 90-91 -8.927928289538695 1101 92-93 -16.83250684658475 1101 94-95 -10.980636631042099 1101 96-97 -9.199025428525141 1101 98-99 -9.227923580640283 1101 100-101 -9.213003906636523 1102 1 0.5422898367395312 1102 2 0.518315626697408 1102 3 0.5200599789543254 1102 4 0.4397377053541831 1102 5 0.4480868853090598 1102 6 0.5109112548678283 1102 7 0.5610059661859879 1102 8 0.5031351430347115 1102 9 0.7106961999389299 1102 10-11 0.6381293318134595 1102 12-13 0.557358985851927 1102 14-15 0.6664376364362141 1102 16-17 0.6281483417485489 1102 18-19 0.6873651820794393 1102 20-21 0.7097589340212522 1102 22-23 0.8202712423461449 1102 24-25 0.7139682682381903 1102 26-27 0.7022530451328208 1102 28-29 0.6795574444375987 1102 30-31 0.7023423847972339 1102 32-33 0.7322191345162423 1102 34-35 0.437959236361948 1102 36-37 0.48592803188025613 1102 38-39 0.5910765631166797 1102 40-41 0.526082098637545 1102 42-43 0.568353894061076 1102 44-45 0.7953377677148907 1102 46-47 0.5918483000617343 1102 48-49 0.6587072876810112 1102 50-51 0.5126669569155453 1102 52-53 0.46905772633386533 1102 54-55 0.35390781143049566 1102 56-57 -2.524540120899161 1102 58-59 -10.016972440954167 1102 60-61 -9.915153273298824 1102 62-63 -3.7804275856686793 1102 64-65 -5.035628894557963 1102 66-67 -6.713450184749295 1102 68-69 -4.171721373935728 1102 70-71 -4.091767637357584 1102 72-73 -4.241838183927907 1102 74-75 -5.115320429871806 1102 76-77 -7.023735364658037 1102 78-79 -9.832267447108183 1102 80-81 -5.564595962778661 1102 82-83 -3.0133955922664235 1102 84-85 -2.5085864448366273 1102 86-87 -2.492872591606833 1102 88-89 -2.7231834889763107 1102 90-91 -8.489016320045288 1102 92-93 -12.949277216112687 1102 94-95 -10.272165447808666 1102 96-97 -9.072620866786771 1102 98-99 -9.087021913835452 1102 100-101 -9.092205431143945 1103 1 0.2667702726394907 1103 2 0.23082999406160098 1103 3 0.2669445451704071 1103 4 0.24896399168863326 1103 5 0.2905100938127063 1103 6 0.2632123223624916 1103 7 0.21560557307659423 1103 8 0.3181471923115353 1103 9 0.3664422100218587 1103 10-11 0.35340052116247733 1103 12-13 0.355094366772704 1103 14-15 0.3910262630177215 1103 16-17 0.3795607924954183 1103 18-19 0.3890920327571905 1103 20-21 0.302801427180448 1103 22-23 0.40365132228183853 1103 24-25 0.4718352337115306 1103 26-27 0.42102157042316435 1103 28-29 0.395914223265585 1103 30-31 0.510205189856137 1103 32-33 0.48615311491014523 1103 34-35 0.346814509279163 1103 36-37 0.32499931988484576 1103 38-39 0.4035616085169451 1103 40-41 0.38905874975474575 1103 42-43 0.2878410363898496 1103 44-45 0.3810680836794518 1103 46-47 0.5396582897542572 1103 48-49 0.45704264415686424 1103 50-51 0.4118306538956418 1103 52-53 0.5183911841478661 1103 54-55 0.484801118100016 1103 56-57 -2.4900962687774104 1103 58-59 -10.231411348353696 1103 60-61 -10.324371835993087 1103 62-63 -3.8782255917751307 1103 64-65 -4.699671845959944 1103 66-67 -6.00792601035268 1103 68-69 -3.932501525295187 1103 70-71 -3.881918722110999 1103 72-73 -4.293213529351789 1103 74-75 -4.8594404106502935 1103 76-77 -6.5722915814120455 1103 78-79 -9.05608858671139 1103 80-81 -4.654204421996049 1103 82-83 -2.390613998895379 1103 84-85 -2.0110791103643884 1103 86-87 -1.791661413349594 1103 88-89 -1.8974830563330194 1103 90-91 -4.765239969592681 1103 92-93 -9.993147890525822 1103 94-95 -8.531065151563272 1103 96-97 -8.134903067100574 1103 98-99 -8.288861235046916 1103 100-101 -8.435396624283124 1104 1 0.18960986861291929 1104 2 0.26208357750118694 1104 3 0.22176054535296075 1104 4 0.14591268396495138 1104 5 0.1643687415616668 1104 6 0.15815571471382128 1104 7 0.23075229085986138 1104 8 0.16579688381482782 1104 9 0.23567725798646677 1104 10-11 0.2353869090838998 1104 12-13 0.2206555512403341 1104 14-15 0.30842259642807335 1104 16-17 0.28209394555143064 1104 18-19 0.2519355081159631 1104 20-21 0.34830918452489357 1104 22-23 0.44135652322544416 1104 24-25 0.4619212647865467 1104 26-27 0.3862112181692652 1104 28-29 0.37748714287388196 1104 30-31 0.40163688272291154 1104 32-33 0.3983527834508962 1104 34-35 0.5121614718060101 1104 36-37 0.37948414606841396 1104 38-39 0.313027594009192 1104 40-41 0.27512961699704164 1104 42-43 0.34746233992037645 1104 44-45 0.23650934950006786 1104 46-47 0.30446099733702425 1104 48-49 0.3761403447652967 1104 50-51 0.3549127970278434 1104 52-53 0.3024901255891095 1104 54-55 0.22109790248892125 1104 56-57 -2.756749956618105 1104 58-59 -10.373794774250303 1104 60-61 -10.559581108865057 1104 62-63 -4.189221937736431 1104 64-65 -5.494326138528354 1104 66-67 -6.275092290507061 1104 68-69 -4.065907026605906 1104 70-71 -4.305930833780248 1104 72-73 -4.349055997781864 1104 74-75 -4.9531831902869445 1104 76-77 -6.521897652650111 1104 78-79 -9.174513385111712 1104 80-81 -4.86175652984296 1104 82-83 -2.7624759041870313 1104 84-85 -2.2260342834962223 1104 86-87 -2.1116915926855953 1104 88-89 -2.0115753514743773 1104 90-91 -4.354209821634061 1104 92-93 -6.5107454670509775 1104 94-95 -7.979506408826422 1104 96-97 -8.112178711691826 1104 98-99 -8.320868588764137 1104 100-101 -8.4282860817682 1105 1 0.32137152862643603 1105 2 0.28107076859745206 1105 3 0.2602123387270261 1105 4 0.24050684194851613 1105 5 0.2773659740222243 1105 6 0.15575420468308465 1105 7 0.28821168115094764 1105 8 0.1742406423969598 1105 9 0.3409768196864391 1105 10-11 0.434496917713858 1105 12-13 0.2768372395669232 1105 14-15 0.46633771671614 1105 16-17 0.3924462860565612 1105 18-19 0.4141020536230684 1105 20-21 0.49058388088202776 1105 22-23 0.5436260331031377 1105 24-25 0.5812022146775035 1105 26-27 0.5544869688271419 1105 28-29 0.6134751459517602 1105 30-31 0.6182958972067993 1105 32-33 0.5543542203607714 1105 34-35 0.47429188200551664 1105 36-37 0.3502810783933583 1105 38-39 0.33265097907978003 1105 40-41 0.40287962018545187 1105 42-43 0.45833609165806166 1105 44-45 0.5609298029344103 1105 46-47 0.5741481505974946 1105 48-49 0.24663990476479114 1105 50-51 0.4009098551886865 1105 52-53 0.44216711845132295 1105 54-55 0.29505901239551235 1105 56-57 1.5354522565632038 1105 58-59 -8.433401026933879 1105 60-61 -9.725024063968 1105 62-63 -3.6152249050828686 1105 64-65 -5.15509318482831 1105 66-67 -6.273861564442452 1105 68-69 -3.9859727503536053 1105 70-71 -3.8260456590341505 1105 72-73 -2.243280512824782 1105 74-75 -4.314931629029097 1105 76-77 -6.358253810744207 1105 78-79 -8.922530832088462 1105 80-81 -4.405350590048638 1105 82-83 -2.10129755306237 1105 84-85 -1.64209685284413 1105 86-87 -1.5984337608890726 1105 88-89 -1.5590001794065422 1105 90-91 -4.029968769373905 1105 92-93 -6.250437764278761 1105 94-95 -7.837816679331083 1105 96-97 -8.190534937805324 1105 98-99 -8.757331239735327 1105 100-101 -8.70768321730111 1106 1 0.2372620513098589 1106 2 0.29347082166175653 1106 3 0.22370158378521765 1106 4 0.2102121074034926 1106 5 0.17897897980121513 1106 6 0.22697360832192004 1106 7 0.1641116549327819 1106 8 0.18212748197790773 1106 9 0.2760684184135229 1106 10-11 0.2669266467562039 1106 12-13 0.26134723072811283 1106 14-15 0.3501236469820981 1106 16-17 0.43105155041256893 1106 18-19 0.37274358774671157 1106 20-21 0.37455516335840855 1106 22-23 0.2858576266179895 1106 24-25 0.40185300208587904 1106 26-27 0.4198977148202374 1106 28-29 0.5088646740719227 1106 30-31 0.6194848398926354 1106 32-33 0.35340799192992023 1106 34-35 0.5019267289275575 1106 36-37 0.4025975002263422 1106 38-39 0.4608114844780502 1106 40-41 0.5667059531121694 1106 42-43 0.42868601779191096 1106 44-45 0.3805061558779883 1106 46-47 0.5184727228254005 1106 48-49 0.32506861647960505 1106 50-51 0.19050536544595076 1106 52-53 0.4082106720015304 1106 54-55 0.3997051025137779 1106 56-57 1.7095077943260009 1106 58-59 -9.001871840780211 1106 60-61 -10.205158408506069 1106 62-63 -3.864888052706828 1106 64-65 -5.675548650844387 1106 66-67 -7.050880206027596 1106 68-69 -4.443882884066239 1106 70-71 -4.304313431259541 1106 72-73 -4.72087791644163 1106 74-75 -5.331353501257153 1106 76-77 -6.803432533841153 1106 78-79 -9.356452131284804 1106 80-81 -4.970198842615897 1106 82-83 -2.5149284272261774 1106 84-85 -2.011617138951795 1106 86-87 -1.9333833576474433 1106 88-89 -1.7893841459791702 1106 90-91 -4.234702501945499 1106 92-93 -6.28075281005065 1106 94-95 -7.815556457779753 1106 96-97 -7.6731489200931495 1106 98-99 -7.124349908794597 1106 100-101 -7.519829782329218 1107 1 0.11519587886682814 1107 2 -0.0555160323897006 1107 3 0.015348804152431228 1107 4 0.06764365767999436 1107 5 0.027286026239728756 1107 6 0.055242087308656096 1107 7 0.08597711155189813 1107 8 0.10274549913066977 1107 9 0.10054712002600752 1107 10-11 0.09528863680669986 1107 12-13 0.04590665011537709 1107 14-15 0.21183672840144396 1107 16-17 0.09952322134366653 1107 18-19 0.1109323994340059 1107 20-21 0.021224031021027656 1107 22-23 0.14007275271254827 1107 24-25 0.29813910279644773 1107 26-27 0.1725079584554834 1107 28-29 0.17137170850499928 1107 30-31 0.21085490359646286 1107 32-33 0.3351270010315446 1107 34-35 0.22573706560490336 1107 36-37 0.06872736148856262 1107 38-39 0.07672543554090794 1107 40-41 0.04157379956269125 1107 42-43 0.05772640856874034 1107 44-45 0.1644966303176716 1107 46-47 0.21127368401032953 1107 48-49 0.09960987765028051 1107 50-51 0.10259652419528464 1107 52-53 0.09007571593390651 1107 54-55 -0.041470248082362104 1107 56-57 1.1649285284581907 1107 58-59 -8.95361881878776 1107 60-61 -10.449069497023622 1107 62-63 -3.9376787717571986 1107 64-65 -5.300391454713139 1107 66-67 -7.133702120443868 1107 68-69 -4.023408838310534 1107 70-71 -3.992301058160713 1107 72-73 -2.455882568546709 1107 74-75 -4.817189529559959 1107 76-77 -6.530082155294862 1107 78-79 -7.462746448213215 1107 80-81 -2.4412446470924216 1107 82-83 -1.199927733162113 1107 84-85 -0.9809526382652365 1107 86-87 -1.0062578333476964 1107 88-89 -0.9517044396620626 1107 90-91 -0.4631183288571741 1107 92-93 -2.9884543887650175 1107 94-95 -5.755625432740924 1107 96-97 -6.670619900711879 1107 98-99 -6.235305334874899 1107 100-101 -7.026170620308404 1108 1 -0.11345641939447404 1108 2 -0.10594567672377764 1108 3 -0.09804850066266013 1108 4 -0.0147667990666136 1108 5 -0.05294493450890059 1108 6 -0.12325403216762965 1108 7 -0.14135234372195526 1108 8 -0.11050375886742358 1108 9 -0.07449461657665779 1108 10-11 -0.08484493317867958 1108 12-13 -0.07635584981229471 1108 14-15 -0.1722407496470595 1108 16-17 -0.12466247294961619 1108 18-19 -0.12153138116614315 1108 20-21 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1.2965281555055341 2316 90-91 2.4048129907595346 2316 92-93 4.44836246621426 2316 94-95 11.44448065280109 2316 96-97 13.435659218168576 2316 98-99 13.09663226244458 2316 100-101 11.840440509992105 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 8.0 3 15.0 4 48.0 5 101.0 6 234.0 7 648.0 8 1449.0 9 2408.0 10 4116.0 11 6523.0 12 9730.0 13 13860.0 14 16976.0 15 23128.0 16 31234.0 17 42528.0 18 44733.0 19 43022.0 20 44308.0 21 44011.0 22 44062.0 23 37778.0 24 38689.0 25 45357.0 26 63921.0 27 94420.0 28 133159.0 29 181536.0 30 225907.0 31 251247.0 32 254447.0 33 235954.0 34 212299.0 35 192014.0 36 158111.0 37 103478.0 38 41354.0 39 3100.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.52469536697723 19.350897473797062 13.991420300198767 22.132986859026943 2 14.89370950300504 22.218064565386904 39.660266520921894 23.22795941068616 3 20.162182448451553 26.92193873636622 30.49931044491704 22.416568370265193 4 12.154254504966715 16.87209670159223 38.80947710676806 32.164171686672994 5 12.479145345210773 39.821528059540604 34.116814831860765 13.582511763387856 6 29.49140806972867 37.41154754521408 18.331744089847245 14.76530029521001 7 26.909473935641092 34.59071957041655 21.176781389554904 17.323025104387447 8 25.350224008816735 34.06086750787451 20.535678515789467 20.05322996751929 9 26.61145699046038 17.33651106442275 20.938556936679323 35.11347500843754 10-11 24.300156505523802 26.57662969266185 28.024050677403224 21.09916312441112 12-13 26.9935557215978 24.251609935776422 27.82047255522007 20.93436178740571 14-15 23.38884766147995 25.366157027862236 27.77475737402129 23.47023793663653 16-17 21.114375264795175 28.8793887025008 28.30293361875466 21.703302413949363 18-19 20.802884603573933 28.49995436351848 30.228925063385574 20.468235969522013 20-21 22.512153649798766 26.89411934557183 29.07848821937834 21.515238785251064 22-23 21.628273404923153 26.94316164551513 28.86095391863735 22.56761103092437 24-25 22.150222276813764 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28.36981940063554 22.26111395620688 50-51 22.211310773182 27.608448526263874 28.681587752675142 21.498652947878988 52-53 22.00192729428876 27.807471055122697 28.577367437048494 21.613234213540046 54-55 21.066706506312094 27.128812857363037 29.15482902835076 22.649651607974107 56-57 21.131551152870667 27.977051454680215 28.779482954743006 22.11191443770611 58-59 20.635566535499798 27.063037450025462 30.020213428157877 22.281182586316866 60-61 21.66581888592113 27.02501516698115 29.223762072328167 22.08540387476955 62-63 22.6477838912464 26.811878082128356 28.4983261190624 22.04201190756284 64-65 21.763504494797616 27.39685804045176 28.999754992616673 21.839882472133958 66-67 20.921340299422695 27.324516330816316 29.239125243240494 22.515018126520495 68-69 21.13496560872336 27.51162111857168 29.206574607843766 22.146838664861196 70-71 21.016048131765857 27.536278065286428 28.826151877754796 22.621521925192916 72-73 20.269456297785855 27.056184805948668 29.465019586222734 23.209339310042736 74-75 20.825865070198297 27.646502789983092 28.918946378690052 22.608685761128555 76-77 19.598616129128068 27.240028397904076 29.909412218955122 23.251943254012737 78-79 20.131675544409024 27.235090132592976 29.589174819140673 23.044059503857323 80-81 21.23955544984593 28.011133038716068 28.2346862106792 22.5146253007588 82-83 21.52434335831595 27.688676829661212 27.794349497988684 22.99263031403415 84-85 21.42785333887144 27.17863690701381 27.727918836210918 23.665590917903828 86-87 21.726268876825795 27.234747456728382 28.030317059998044 23.008666606447782 88-89 22.041225892037144 26.670691746884817 27.911833735841356 23.376248625236688 90-91 20.851305657375047 26.931321569630324 28.836965684463767 23.380407088530863 92-93 19.86681513462655 27.7544853384728 29.235927475804402 23.142772051096248 94-95 18.907818802800637 27.9518720721464 28.994131088422286 24.146178036630676 96-97 18.688427788022672 27.810500128262838 28.47009055840315 25.03098152531134 98-99 19.2506556001841 27.55068997536254 28.277282375803857 24.921372048649502 100-101 19.96641584726458 27.34775791257935 28.353508683757937 24.332317556398124 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 910.0 1 657.5 2 423.5 3 492.0 4 555.5 5 652.0 6 852.5 7 1225.5 8 1633.0 9 1919.5 10 2162.0 11 2379.0 12 2678.0 13 2973.5 14 3245.5 15 3580.0 16 3942.5 17 4283.5 18 4542.5 19 5020.5 20 5691.0 21 6379.5 22 7347.5 23 8657.0 24 10288.0 25 12591.5 26 15576.5 27 19323.5 28 23665.0 29 28458.0 30 33788.5 31 39318.5 32 45262.0 33 51751.5 34 58165.0 35 64251.0 36 70154.5 37 74899.5 38 77798.0 39 80068.5 40 82246.0 41 85012.0 42 88163.5 43 91942.0 44 97605.0 45 104691.5 46 114376.0 47 125553.0 48 136036.0 49 130403.5 50 112100.0 51 99095.5 52 88288.5 53 79491.0 54 71983.5 55 63835.0 56 56378.5 57 49095.0 58 42087.0 59 35417.5 60 28829.5 61 22865.5 62 17605.5 63 13172.0 64 9717.0 65 7101.0 66 4925.0 67 3369.0 68 2314.5 69 1577.5 70 1059.5 71 768.0 72 572.5 73 435.0 74 316.0 75 191.0 76 95.0 77 40.0 78 20.0 79 12.5 80 6.0 81 3.0 82 2.5 83 2.0 84 2.0 85 2.0 86 1.0 87 0.0 88 0.0 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.022638688422484034 2 0.007256474419226937 3 1.5117655040056116E-4 4 0.0 5 0.1442602232197355 6 0.0 7 3.779413760014029E-5 8 0.00132279481600491 9 0.0 10-11 0.0 12-13 0.0 14-15 7.369856832027357E-4 16-17 0.0 18-19 1.3227948160049103E-4 20-21 0.0 22-23 2.2676482560084177E-4 24-25 1.8897068800070146E-5 26-27 1.3227948160049103E-4 28-29 0.0031558104896117142 30-31 0.012396477132846015 32-33 0.00264558963200982 34-35 0.004365222892816204 36-37 0.10901718990760467 38-39 0.10873373387560362 40-41 0.12103572566444928 42-43 0.1232466827140575 44-45 0.11888145982124128 46-47 0.11181395609001506 48-49 0.11200292677801575 50-51 0.11033998472360959 52-53 0.08732335492512415 54-55 0.07485128951707784 56-57 0.5399459468244043 58-59 1.5481045673081466 60-61 0.512110564481901 62-63 0.004365222892816204 64-65 0.11850351844523987 66-67 1.8790111390661748 68-69 2.8284943609256996 70-71 2.74236152133498 72-73 2.587518939587205 74-75 1.9818678845449567 76-77 1.3819615384179298 78-79 0.9248036500066328 80-81 0.05109767403538968 82-83 2.4566189440091194E-4 84-85 1.5117655040056116E-4 86-87 3.779413760014029E-4 88-89 1.1338241280042089E-4 90-91 0.24331865786970322 92-93 0.3818341721742174 94-95 1.968167509664906 96-97 2.393332660597684 98-99 1.378427786552317 100-101 0.9236320317410285 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2645913.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 42.948875118702205 #Duplication Level Percentage of deduplicated Percentage of total 1 68.67000121458817 29.492993065664763 2 15.313102563887549 13.153610593925698 3 6.309622211372681 8.129735292073049 4 3.11450198810526 5.350574277763302 5 1.797858865811497 3.8608007954394776 6 1.128778823624681 2.90878684394952 7 0.7605080657541058 2.2864076159987197 8 0.5308559818934032 1.823973381788465 9 0.3783354812697471 1.462417500422562 >10 1.8031856763340706 13.590505168478947 >50 0.115967144872149 3.4077229823159407 >100 0.06799654650243725 5.365374119930286 >500 0.0045100689066679125 1.2495323147475221 >1k 0.003625741670063953 3.289864116570631 >5k 7.958945129408678E-4 2.4160004885668775 >10k+ 3.5373089464038566E-4 2.2117014423641574 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 17885 0.6759481509785091 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 14969 0.5657404457365001 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 13032 0.4925332012050283 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12346 0.466606422811332 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 9523 0.35991357236613597 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9094 0.3436998873356758 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 7320 0.27665308723302695 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 6851 0.2589276366985611 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6802 0.2570757239561543 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 6780 0.2562442529289512 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 6353 0.24010615617369124 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 5470 0.2067339326727674 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 5418 0.2047686375175601 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 4839 0.18288583184707885 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 4638 0.17528921018945068 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 3717 0.14048080945972147 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 3647 0.13783521982771166 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 3335 0.12604344889646787 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 3097 0.11704844414763449 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 3065 0.11583903174443 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 3036 0.11474300175402592 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 2974 0.11239976522281724 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 2922 0.11043447006760994 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 2829 0.10691961527079688 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 3.779413760014029E-5 7 0.0 0.0 0.0 0.0 3.779413760014029E-5 8 0.0 0.0 0.0 0.0 3.779413760014029E-5 9 0.0 0.0 0.0 0.0 3.779413760014029E-5 10-11 0.0 0.0 0.0 3.779413760014029E-5 3.779413760014029E-5 12-13 0.0 0.0 0.0 7.558827520028058E-5 3.779413760014029E-5 14-15 0.0 0.0 0.0 9.448534400035072E-5 1.5117655040056116E-4 16-17 0.0 0.0 0.0 1.7007361920063133E-4 1.8897068800070147E-4 18-19 0.0 0.0 0.0 1.8897068800070147E-4 1.8897068800070147E-4 20-21 0.0 0.0 0.0 2.6455896320098205E-4 1.8897068800070147E-4 22-23 0.0 0.0 0.0 5.669120640021043E-4 1.8897068800070147E-4 24-25 0.0 0.0 0.0 0.0012661036096046998 1.8897068800070147E-4 26-27 0.0 0.0 0.0 0.0019841922240073657 1.8897068800070147E-4 28-29 0.0 0.0 0.0 0.0055557382272206236 1.8897068800070147E-4 30-31 0.0 0.0 0.0 0.016100302617659764 1.8897068800070147E-4 32-33 0.0 0.0 0.0 0.03762406398093966 2.2676482560084175E-4 34-35 0.0 0.0 0.0 0.07027819886746087 2.2676482560084175E-4 36-37 0.0 0.0 0.0 0.11948616602284354 2.2676482560084175E-4 38-39 0.0 0.0 0.0 0.18713767232709466 2.2676482560084175E-4 40-41 0.0 0.0 0.0 0.26455896320098204 2.2676482560084175E-4 42-43 0.0 0.0 0.0 0.3420558423500697 2.2676482560084175E-4 44-45 0.0 0.0 0.0 0.4341601556816116 2.2676482560084175E-4 46-47 0.0 0.0 0.0 0.5402671969940054 2.2676482560084175E-4 48-49 0.0 0.0 0.0 0.6434829867799886 2.2676482560084175E-4 50-51 0.0 3.779413760014029E-5 0.0 0.7413509060955519 2.2676482560084175E-4 52-53 0.0 7.558827520028058E-5 0.0 0.8336063959774944 2.2676482560084175E-4 54-55 0.0 7.558827520028058E-5 0.0 0.9315688006370579 2.2676482560084175E-4 56-57 0.0 7.558827520028058E-5 0.0 1.0145080356005658 2.2676482560084175E-4 58-59 0.0 7.558827520028058E-5 0.0 1.0750731411047907 2.2676482560084175E-4 60-61 0.0 7.558827520028058E-5 0.0 1.1304415526889962 2.2676482560084175E-4 62-63 0.0 7.558827520028058E-5 0.0 1.1784212103723744 2.2676482560084175E-4 64-65 0.0 7.558827520028058E-5 0.0 1.2204860855213304 2.2676482560084175E-4 66-67 1.8897068800070146E-5 7.558827520028058E-5 0.0 1.257770002263869 2.2676482560084175E-4 68-69 3.779413760014029E-5 7.558827520028058E-5 0.0 1.3092078235376596 2.2676482560084175E-4 70-71 3.779413760014029E-5 7.558827520028058E-5 0.0 1.3903329398963609 2.2676482560084175E-4 72-73 3.779413760014029E-5 7.558827520028058E-5 0.0 1.5046224119991851 2.2676482560084175E-4 74-75 3.779413760014029E-5 7.558827520028058E-5 0.0 1.6545328587901416 2.2676482560084175E-4 76-77 3.779413760014029E-5 7.558827520028058E-5 0.0 1.876082093402164 2.2676482560084175E-4 78-79 3.779413760014029E-5 7.558827520028058E-5 0.0 2.2167584497298285 2.456618944009119E-4 80-81 3.779413760014029E-5 7.558827520028058E-5 0.0 2.630453079900964 2.8345603200105216E-4 82-83 3.779413760014029E-5 7.558827520028058E-5 0.0 3.0380250597808773 3.401472384012626E-4 84-85 3.779413760014029E-5 9.448534400035072E-5 0.0 3.378682519039742 3.401472384012626E-4 86-87 3.779413760014029E-5 1.8897068800070147E-4 0.0 3.6419942756999193 3.401472384012626E-4 88-89 3.779413760014029E-5 1.8897068800070147E-4 0.0 3.8775273412239937 3.401472384012626E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 16875 0.0 26.343555 1 TACATGG 16565 0.0 26.26152 2 ACATGGG 16545 0.0 25.068237 3 CATGGGA 12020 0.0 22.520502 4 ATGGGAG 4510 0.0 21.92085 5 TACCTGG 1505 0.0 21.584995 2 CATGGGG 4515 0.0 21.16669 4 AGTACTT 10320 0.0 20.953289 12-13 GAGTACT 10030 0.0 20.481161 12-13 ATGGGAT 4715 0.0 19.170534 5 GTACTTT 11065 0.0 18.671598 14-15 AGTACAT 12670 0.0 17.3532 2 GCTTATA 3225 0.0 16.93848 1 TACTTTT 12745 0.0 16.8374 14-15 TAGGACG 315 1.485314E-7 16.439812 4 TACGCGG 195 4.0654413E-9 16.205328 64-65 ACTTTTT 13110 0.0 16.082073 16-17 GTAGGAC 675 0.0 16.041271 3 GTATAGC 960 0.0 15.697299 1 ATGGGGG 2525 0.0 15.661472 5 >>END_MODULE