FastQCFastQC Report
Fri 27 May 2016
SRR939646_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939646_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2647026
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA76140.28764356678022807No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA57130.21582712070074114No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT49520.18707787532120954No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48890.18469784580884357No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33070.12493266027609852No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT27470.10377684238840118No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA26530.10022568724296625No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG139600.032.2362141
TACATGG163100.026.2738272
ACATGGG162200.025.6918853
CATGGGA119250.023.8479234
GAGTACT91850.023.27863712-13
ATGGGAG42700.022.6600275
GTATAGG6551.0913936E-1121.5183351
AGTACTT98150.021.20736712-13
GTACTTT102700.020.9607514-15
CATGGGG46150.020.7795814
GGTCGGG7002.7284841E-1120.1350141
ATGGGAT48000.019.9613175
GCCTACG3801.1965962E-418.5454061
GCTTATA23150.018.2650221
ACTTTTT131250.017.60887316-17
GTCGGGA5650.017.5606612
GTATAGT8052.055458E-1017.5087071
GTATATA16400.017.1884271
GTGTAGC13250.016.715861
TACTTTT124650.016.531214-15
Produced by FastQC (version 0.11.5)