##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939646_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2647026 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 15.279538999616928 2.0 2.0 31.0 2.0 33.0 2 22.57525955544071 16.0 16.0 31.0 16.0 34.0 3 27.041312778945127 28.0 25.0 31.0 16.0 34.0 4 32.27244046715068 35.0 32.0 35.0 25.0 37.0 5 32.89988235854125 35.0 33.0 35.0 26.0 37.0 6 33.31038078205503 35.0 33.0 37.0 28.0 37.0 7 33.48380333249466 35.0 33.0 37.0 28.0 37.0 8 33.418252408552085 35.0 33.0 37.0 27.0 37.0 9 34.752847535309435 37.0 34.0 39.0 27.0 39.0 10-11 34.58841734081947 37.0 34.0 39.0 27.0 39.0 12-13 34.61060053811334 37.0 34.0 39.0 27.0 39.0 14-15 35.236990872020144 38.0 33.5 40.0 27.0 41.0 16-17 34.88482697185445 38.0 33.0 40.0 25.0 41.0 18-19 35.22034162112499 38.0 33.5 40.0 27.0 41.0 20-21 35.2262867837339 38.0 34.0 40.0 26.0 41.0 22-23 35.05961010583198 38.0 33.5 40.0 25.5 41.0 24-25 34.85929662194478 38.0 33.0 40.0 25.0 41.0 26-27 34.91171601638972 38.0 33.5 40.0 25.0 41.0 28-29 34.66364497364212 38.0 33.0 40.0 24.0 41.0 30-31 34.546257951376376 38.0 33.0 40.0 24.0 41.0 32-33 34.23608098295975 38.0 33.0 40.0 24.0 41.0 34-35 33.94991076778241 38.0 33.0 40.0 23.0 41.0 36-37 33.63588325161899 38.0 32.0 40.0 19.5 41.0 38-39 33.40708780344431 37.0 31.5 40.0 18.5 41.0 40-41 33.020148272060794 37.0 31.0 40.0 17.0 41.0 42-43 32.77971145731096 37.0 31.0 40.0 15.0 41.0 44-45 32.43035561418739 37.0 30.5 40.0 14.5 41.0 46-47 32.05591539335088 37.0 30.0 39.0 10.5 41.0 48-49 31.66417538021916 36.5 30.0 39.0 9.0 40.0 50-51 30.992770188128112 35.5 29.0 38.5 8.0 40.0 52-53 30.99777486129717 35.5 29.0 39.0 7.5 40.0 54-55 31.139701310074024 36.0 29.0 39.0 6.5 40.0 56-57 30.88298112674375 36.0 28.5 39.0 2.0 40.0 58-59 30.503168272619913 35.0 28.0 39.0 2.0 40.0 60-61 29.89318861998333 35.0 27.5 38.0 2.0 40.0 62-63 29.4924437840807 34.0 27.0 38.0 2.0 40.0 64-65 28.88629295669933 34.0 26.0 38.0 2.0 40.0 66-67 28.28810937255622 33.5 25.5 37.0 2.0 39.0 68-69 27.96530785870634 33.0 25.5 37.0 2.0 39.0 70-71 27.418927505812185 33.0 24.5 36.0 2.0 39.0 72-73 26.81004228896883 32.0 22.5 36.0 2.0 38.5 74-75 26.23930535627531 32.0 21.5 35.0 2.0 38.0 76-77 25.578485439886123 31.0 19.5 35.0 2.0 37.0 78-79 24.90895045987459 31.0 18.0 34.5 2.0 36.0 80-81 24.17590307008696 30.5 12.5 34.0 2.0 36.0 82-83 23.492288704379934 30.0 7.5 34.0 2.0 35.0 84-85 22.80795504086473 29.0 4.0 34.0 2.0 35.0 86-87 22.096207970756616 29.0 2.0 34.0 2.0 35.0 88-89 21.224811354327457 28.5 2.0 33.0 2.0 35.0 90-91 20.23596217037536 26.5 2.0 33.0 2.0 34.0 92-93 19.191517008144235 25.5 2.0 32.0 2.0 34.0 94-95 18.207956967555287 24.5 2.0 32.0 2.0 34.0 96-97 17.27865102193934 21.5 2.0 31.5 2.0 34.0 98-99 16.221064696757793 10.0 2.0 31.0 2.0 34.0 100-101 14.66950343517593 2.0 2.0 30.0 2.0 33.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 15.036056693125413 1101 2 8.171533932642252 1101 3 3.863364059733396 1101 4 2.5016707819116206 1101 5 1.8242000080796288 1101 6 1.467639555768848 1101 7 1.1612649544463665 1101 8 1.1980590451157624 1101 9 1.154469286317422 1101 10-11 1.1119180278459808 1101 12-13 0.8638385760855911 1101 14-15 1.0066176630457804 1101 16-17 1.017182679230622 1101 18-19 1.2066294651944816 1101 20-21 1.0189581801943675 1101 22-23 1.1609022232538493 1101 24-25 1.3435221531371369 1101 26-27 1.3613245838746693 1101 28-29 1.383268752596834 1101 30-31 1.364273651485611 1101 32-33 1.3632556523160417 1101 34-35 1.488618128712531 1101 36-37 1.3734547187822557 1101 38-39 1.3116547324634453 1101 40-41 1.3868753355427685 1101 42-43 1.320332391792256 1101 44-45 1.1935091791107268 1101 46-47 1.2066043386493988 1101 48-49 1.0709371165800334 1101 50-51 1.090207487808712 1101 52-53 1.2323414580263155 1101 54-55 1.2887373389089873 1101 56-57 1.049453419633629 1101 58-59 1.203490614601698 1101 60-61 1.2740419168103827 1101 62-63 1.0708473504077745 1101 64-65 1.0533708148132739 1101 66-67 1.0039572360581523 1101 68-69 0.8473769780730471 1101 70-71 0.5888349436789717 1101 72-73 0.5832181398204526 1101 74-75 0.49582924830252395 1101 76-77 0.5738641259544757 1101 78-79 0.44112104011096065 1101 80-81 0.5605435594436052 1101 82-83 0.7348806859081627 1101 84-85 0.41119201333201616 1101 86-87 0.4206457936722181 1101 88-89 0.31447251171116264 1101 90-91 0.3293218411861183 1101 92-93 0.3444534846073175 1101 94-95 0.3890439997160051 1101 96-97 -0.3229430695473994 1101 98-99 -0.5906698914290871 1101 100-101 -0.5466946041172402 1102 1 14.942304716009478 1102 2 8.069579588596035 1102 3 3.8044465657628983 1102 4 2.2473740743004242 1102 5 1.6584718124433095 1102 6 1.2421323484100455 1102 7 1.190002404667915 1102 8 1.055376000637665 1102 9 1.1145662070645201 1102 10-11 1.1634982622954766 1102 12-13 0.7898550660738124 1102 14-15 0.8386735270875079 1102 16-17 1.0729412747772074 1102 18-19 0.9714414008050056 1102 20-21 1.0164135618759005 1102 22-23 0.8629954421668558 1102 24-25 0.7016483520265879 1102 26-27 0.9202878459757358 1102 28-29 1.08407911773228 1102 30-31 0.8756084431172226 1102 32-33 1.0499701062955182 1102 34-35 1.0916065184147001 1102 36-37 1.091143876895437 1102 38-39 0.8917922612087423 1102 40-41 0.8924175420602438 1102 42-43 0.6746481694578605 1102 44-45 0.7458733049730881 1102 46-47 0.5057814097133928 1102 48-49 0.38092690849208566 1102 50-51 0.38904264401489286 1102 52-53 0.8133030374755243 1102 54-55 0.611655730293819 1102 56-57 0.6661657422540799 1102 58-59 0.5190511059360361 1102 60-61 0.817670835987002 1102 62-63 0.41844059736498096 1102 64-65 0.45183264666026446 1102 66-67 0.4167220574166137 1102 68-69 0.14438836401729205 1102 70-71 0.36797230415908544 1102 72-73 0.36894184838515187 1102 74-75 0.34117200448627116 1102 76-77 0.21330324200136275 1102 78-79 0.27288838544721017 1102 80-81 0.485962371491393 1102 82-83 0.4319032895314727 1102 84-85 0.276928285697263 1102 86-87 0.0781224889439649 1102 88-89 -0.14113262598584697 1102 90-91 -0.014795271383295727 1102 92-93 0.1463346893860482 1102 94-95 0.3389072562082376 1102 96-97 -0.37500611290372987 1102 98-99 -0.7646621512305352 1102 100-101 -0.6697637611086602 1103 1 14.85420487532971 1103 2 7.94179838838361 1103 3 3.7707839706133157 1103 4 2.216242192551441 1103 5 1.6655010211528136 1103 6 1.23154782017496 1103 7 0.8318221957813847 1103 8 0.900105333262907 1103 9 1.0733539571087718 1103 10-11 1.0247668054004464 1103 12-13 0.6531338764827339 1103 14-15 0.8035267754500524 1103 16-17 0.9046070563314643 1103 18-19 0.8361179807306485 1103 20-21 0.8637242363315565 1103 22-23 0.8894921672509426 1103 24-25 1.1287076545580135 1103 26-27 1.0458081150499083 1103 28-29 1.2494644956752907 1103 30-31 1.0005371030570416 1103 32-33 0.9122568827161217 1103 34-35 0.8500363290006447 1103 36-37 0.9890231475628113 1103 38-39 1.0245092022602549 1103 40-41 0.946635633757893 1103 42-43 0.8903985677584387 1103 44-45 0.6912795715689484 1103 46-47 0.6195682746452391 1103 48-49 0.5732887424450013 1103 50-51 0.4979128865356941 1103 52-53 0.7800964522392029 1103 54-55 0.6409225031739716 1103 56-57 0.8233733896252957 1103 58-59 0.7195878381010203 1103 60-61 0.775608524073391 1103 62-63 0.6142026791493684 1103 64-65 0.5097887450847658 1103 66-67 0.6089500623732427 1103 68-69 0.35761265150180677 1103 70-71 0.3067774024065635 1103 72-73 0.23651876465351407 1103 74-75 -0.004277809640125696 1103 76-77 0.029242346267604802 1103 78-79 -0.13169295638320477 1103 80-81 -0.10197390041514254 1103 82-83 -0.08355006504455886 1103 84-85 -0.2611974002648516 1103 86-87 -0.29691061426014187 1103 88-89 -0.4081172250299261 1103 90-91 -0.3607265866603342 1103 92-93 -0.18926276149020893 1103 94-95 -0.31775268360411957 1103 96-97 -0.7306456951306721 1103 98-99 -1.0123877663836574 1103 100-101 -0.8883068660829903 1104 1 14.93089835632589 1104 2 8.211761159134042 1104 3 3.8661072080176027 1104 4 2.4229461838923356 1104 5 1.7968073983828532 1104 6 1.3151842631253317 1104 7 1.1130596627746812 1104 8 1.0686892176736507 1104 9 1.4012502659466008 1104 10-11 1.0073777740561738 1104 12-13 0.8213094115083521 1104 14-15 1.0226651452969548 1104 16-17 1.1009458847620124 1104 18-19 0.9936577557738246 1104 20-21 1.0902145991224828 1104 22-23 1.1494735185459248 1104 24-25 1.201563905486701 1104 26-27 1.1304666924318099 1104 28-29 1.2354821246996508 1104 30-31 1.1433381303716672 1104 32-33 1.1583041206493334 1104 34-35 1.1687776641006948 1104 36-37 1.2917368885775602 1104 38-39 1.1863361346437031 1104 40-41 1.2761153785105535 1104 42-43 1.085835973952591 1104 44-45 0.771353948275511 1104 46-47 0.7781451794236958 1104 48-49 0.8466753500029043 1104 50-51 0.7648081634239787 1104 52-53 0.7117982965252416 1104 54-55 0.7201165192298546 1104 56-57 0.7728489629570987 1104 58-59 0.6958572246431487 1104 60-61 0.6219947921921474 1104 62-63 0.543625782550162 1104 64-65 0.4796174441876353 1104 66-67 0.5116644849927034 1104 68-69 0.44519217773200026 1104 70-71 0.3749218562571528 1104 72-73 0.5252576323444984 1104 74-75 0.43090137742356305 1104 76-77 0.15499950686594488 1104 78-79 0.1847863371471412 1104 80-81 0.2101086337366631 1104 82-83 0.29791219518394385 1104 84-85 0.24007501941825282 1104 86-87 0.1961469009089747 1104 88-89 0.21681928349081758 1104 90-91 0.25299895565845887 1104 92-93 0.3057280572942709 1104 94-95 0.28155003477180784 1104 96-97 -0.2837330236900435 1104 98-99 -0.4942379278558171 1104 100-101 -0.4141086717902045 1105 1 14.690921592019276 1105 2 7.8169831430860555 1105 3 3.8341882311266 1105 4 2.294491115352521 1105 5 1.4904780666779445 1105 6 1.1270772242808675 1105 7 0.8047721272231172 1105 8 0.8660197351106618 1105 9 0.95916042884663 1105 10-11 0.7063447034622357 1105 12-13 0.7437434279855211 1105 14-15 0.8358744791210029 1105 16-17 1.143942820962991 1105 18-19 0.9804789783314476 1105 20-21 1.01263517025658 1105 22-23 0.8497298630421071 1105 24-25 0.9794390783864557 1105 26-27 0.9209184118102485 1105 28-29 1.1676525094384758 1105 30-31 1.0971909034636411 1105 32-33 0.7075822395107139 1105 34-35 0.8668341899284044 1105 36-37 0.8674312937228024 1105 38-39 0.8289936996827052 1105 40-41 1.0199325609098082 1105 42-43 1.0169586922076306 1105 44-45 0.8614828322571881 1105 46-47 0.6184974500879505 1105 48-49 0.842620207774587 1105 50-51 0.7739267148267288 1105 52-53 0.8856388898755903 1105 54-55 0.806881525537122 1105 56-57 0.5950607232307803 1105 58-59 0.6426443027185798 1105 60-61 0.6764078280476014 1105 62-63 0.5559962339939482 1105 64-65 0.6100911202795558 1105 66-67 0.6120147729705465 1105 68-69 0.7706084783308036 1105 70-71 0.6872395163339959 1105 72-73 0.699995652175911 1105 74-75 0.7719768202469446 1105 76-77 0.7121646238397616 1105 78-79 0.619951153640951 1105 80-81 0.5699006630429615 1105 82-83 0.40581194296001044 1105 84-85 0.4137186819725187 1105 86-87 0.4583075447977585 1105 88-89 0.6349997553139914 1105 90-91 0.7826626602023303 1105 92-93 0.931933271759533 1105 94-95 0.9148387375932039 1105 96-97 0.5559608265170617 1105 98-99 0.18201480017753724 1105 100-101 0.2557031348799459 1106 1 14.614438683344602 1106 2 7.785639543113298 1106 3 3.4046092010051296 1106 4 2.1537832811090496 1106 5 1.503107743805721 1106 6 1.0423847086884592 1106 7 0.8983450412884082 1106 8 0.8709651399113199 1106 9 0.9964170788169824 1106 10-11 0.8115469150416672 1106 12-13 0.5070518822822052 1106 14-15 0.7332231101624131 1106 16-17 0.8455737604777056 1106 18-19 0.7844616612970441 1106 20-21 0.7229060158763119 1106 22-23 0.7438475543576075 1106 24-25 0.7943581583803905 1106 26-27 0.5829744974814872 1106 28-29 0.6478871254505734 1106 30-31 0.589479506448491 1106 32-33 0.7499709332543745 1106 34-35 0.7031170965967704 1106 36-37 0.7757455583784534 1106 38-39 0.887245848142797 1106 40-41 0.7232212082445102 1106 42-43 0.5023434351183838 1106 44-45 0.6260573033191648 1106 46-47 0.8463694463752347 1106 48-49 0.9877426344930527 1106 50-51 0.9380890226242258 1106 52-53 0.7412042158919014 1106 54-55 0.8067778445519593 1106 56-57 0.746108475353104 1106 58-59 0.7679138996787174 1106 60-61 0.7812853353323277 1106 62-63 0.6882162847745299 1106 64-65 0.7905216412161202 1106 66-67 0.7905363362395725 1106 68-69 0.6823806457346109 1106 70-71 0.7279640345704976 1106 72-73 0.7029738241276924 1106 74-75 0.571687199855301 1106 76-77 0.6661379880560823 1106 78-79 0.5255349983615503 1106 80-81 0.649146990056618 1106 82-83 0.8080907335394691 1106 84-85 0.5696418822791678 1106 86-87 0.4755697358600983 1106 88-89 0.509720588816716 1106 90-91 0.7075654205056203 1106 92-93 0.9866344619723648 1106 94-95 0.799142043610896 1106 96-97 0.6286023909898724 1106 98-99 0.604648854213611 1106 100-101 0.6275313942906937 1107 1 14.651745473348718 1107 2 7.976615391003005 1107 3 3.6547700127306904 1107 4 2.14517570915951 1107 5 1.4325051474551458 1107 6 1.0115900921702234 1107 7 0.8181360804152646 1107 8 0.7081253502188787 1107 9 0.6054773976246324 1107 10-11 0.7639495774002967 1107 12-13 0.5450387107689139 1107 14-15 0.6378710026233705 1107 16-17 0.8630438311328419 1107 18-19 0.8070790462050681 1107 20-21 0.7457366899286697 1107 22-23 0.6300437997545174 1107 24-25 0.8274550223859549 1107 26-27 0.8281628984288716 1107 28-29 0.963960212785743 1107 30-31 0.8556134114774991 1107 32-33 0.7062885513330315 1107 34-35 0.7207697861186801 1107 36-37 0.6803420574013401 1107 38-39 0.6768865761289291 1107 40-41 0.9258791799406296 1107 42-43 0.7984552090872441 1107 44-45 0.5239285947661259 1107 46-47 0.47507385478447617 1107 48-49 0.42594253532794824 1107 50-51 0.2925621901484696 1107 52-53 0.3808822710723838 1107 54-55 0.594670047899605 1107 56-57 0.42197701170369584 1107 58-59 0.6778243909285706 1107 60-61 0.7233361094985966 1107 62-63 0.4520253700121408 1107 64-65 0.24222175655166822 1107 66-67 0.33025090244069943 1107 68-69 0.2884438216603442 1107 70-71 0.15033596798908633 1107 72-73 0.14301936151941064 1107 74-75 0.10721077783840371 1107 76-77 0.24671747903914465 1107 78-79 0.3145648713394138 1107 80-81 0.3444027763456141 1107 82-83 0.30576387331380417 1107 84-85 0.286207772747634 1107 86-87 0.2519416473440188 1107 88-89 0.34428275243199735 1107 90-91 0.746500732107986 1107 92-93 0.9677738353652039 1107 94-95 0.8825338906477036 1107 96-97 0.659116384033009 1107 98-99 0.5105447910458487 1107 100-101 0.44522356621564363 1108 1 14.32676995691654 1108 2 7.60864612955768 1108 3 3.443379396276139 1108 4 -0.11033650642370674 1108 5 0.2759767000530928 1108 6 0.5579418722195726 1108 7 0.5678194376985672 1108 8 0.5784249428960564 1108 9 0.5940559769912497 1108 10-11 0.5811878701288862 1108 12-13 0.21863311400711893 1108 14-15 0.34547145626265774 1108 16-17 0.6074188546247044 1108 18-19 0.5330141691273269 1108 20-21 0.47152079156875004 1108 22-23 -0.19645013252011978 1108 24-25 0.3777086242772114 1108 26-27 0.39227587091826166 1108 28-29 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-0.47214493392618806 2312 14-15 0.3025257115762372 2312 16-17 0.048086751435448605 2312 18-19 0.12906026131580717 2312 20-21 0.29681752857063515 2312 22-23 0.48841222577618026 2312 24-25 0.5853454237826057 2312 26-27 0.6193667120997901 2312 28-29 -0.19553712326312933 2312 30-31 0.36538690695855536 2312 32-33 0.504403448984263 2312 34-35 -0.328346704524769 2312 36-37 0.27950293761205813 2312 38-39 0.5862443147217036 2312 40-41 0.6988641694341737 2312 42-43 0.45033008544625375 2312 44-45 0.7142894337035628 2312 46-47 0.5828187378076919 2312 48-49 0.6610487159577048 2312 50-51 0.8214206026639701 2312 52-53 0.42697372696939695 2312 54-55 0.5589714754523882 2312 56-57 0.5007972325712267 2312 58-59 0.5087463066096269 2312 60-61 0.7023878347707999 2312 62-63 0.6115512718901925 2312 64-65 0.5992958331136293 2312 66-67 0.47000169068445885 2312 68-69 0.744645076525476 2312 70-71 0.8119951313646219 2312 72-73 0.5631332601168388 2312 74-75 0.692181827668982 2312 76-77 0.6409662600573114 2312 78-79 0.9718284172556082 2312 80-81 0.7706642145075762 2312 82-83 0.8591269774349257 2312 84-85 1.1031943536175532 2312 86-87 1.0883589937620037 2312 88-89 1.3587545477441552 2312 90-91 1.8075476980245107 2312 92-93 2.2019675830150156 2312 94-95 2.120884803219184 2312 96-97 1.7733812011574557 2312 98-99 1.781139888249303 2312 100-101 2.0966035323706205 2313 1 -14.195980998276237 2313 2 -7.402653168046616 2313 3 -3.514763505673322 2313 4 -1.1120637364165376 2313 5 -0.48143585586011994 2313 6 -0.9830154037200387 2313 7 -0.34185814938345516 2313 8 -0.07356175654037855 2313 9 -2.382204875798436 2313 10-11 -0.361830053675952 2313 12-13 -0.6617530290113152 2313 14-15 -0.791005208782039 2313 16-17 -0.7523679507440306 2313 18-19 -0.04198744596659765 2313 20-21 -0.8959506251738034 2313 22-23 -1.7870044048647955 2313 24-25 -1.0836649118591097 2313 26-27 -0.20952525729319404 2313 28-29 0.25536426603510165 2313 30-31 -1.0106237322456906 2313 32-33 -0.05734612517619553 2313 34-35 -0.87780225890927 2313 36-37 -1.1872762659769975 2313 38-39 -0.5889056415951686 2313 40-41 -0.016415523578473312 2313 42-43 0.24096986189969272 2313 44-45 0.13558006464450756 2313 46-47 -0.46320327815468687 2313 48-49 0.126046514445612 2313 50-51 -0.6153105905008864 2313 52-53 0.11592999355831424 2313 54-55 -0.6425186842198229 2313 56-57 -0.15328063469053177 2313 58-59 0.032380217342890205 2313 60-61 -1.390822272788789 2313 62-63 -0.18219544503784846 2313 64-65 0.15357783198142272 2313 66-67 -1.0134786064660304 2313 68-69 -0.04973905861896455 2313 70-71 0.20412209076823729 2313 72-73 -0.5153470170485939 2313 74-75 -0.11499632188700204 2313 76-77 0.5966538607583445 2313 78-79 0.46897912627293437 2313 80-81 0.5580807689949445 2313 82-83 0.5617536558906728 2313 84-85 0.6094823370640334 2313 86-87 0.5081283935223091 2313 88-89 0.9575106671168108 2313 90-91 1.285741158029463 2313 92-93 1.7621641602685258 2313 94-95 1.6761375500604387 2313 96-97 1.2564194603451675 2313 98-99 1.3484028144280735 2313 100-101 1.3642701672715596 2314 1 -14.195980998276237 2314 2 -7.2502908951907 2314 3 -3.4765827273102623 2314 4 -1.419737353321473 2314 5 -1.359935062170944 2314 6 -6.247309041705318 2314 7 -1.9453496344528176 2314 8 -4.15283213685462 2314 9 -1.504666380885915 2314 10-11 -0.4097310022917142 2314 12-13 -2.899612531134565 2314 14-15 -2.7307908417108564 2314 16-17 -2.425356213709712 2314 18-19 -1.1469607261474764 2314 20-21 -1.8685033065663887 2314 22-23 -1.126732516813341 2314 24-25 -2.876797453414234 2314 26-27 -4.793852210046669 2314 28-29 -1.9762093501972586 2314 30-31 -1.8229072479049435 2314 32-33 -4.017878918059992 2314 34-35 -2.7366923943707526 2314 36-37 -0.7888808109457983 2314 38-39 -1.3246338889123237 2314 40-41 -2.2389722884482346 2314 42-43 -1.3298816695964106 2314 44-45 -2.6606309916608204 2314 46-47 -2.3043840937761395 2314 48-49 -1.1535257335816915 2314 50-51 -1.9666333755454737 2314 52-53 -0.8835069136235596 2314 54-55 -0.38891227996113287 2314 56-57 -0.9955020163747967 2314 58-59 -1.2711837134154322 2314 60-61 -0.38096753461547195 2314 62-63 -2.85309147309032 2314 64-65 -1.0941328853152363 2314 66-67 -2.044382628525561 2314 68-69 -1.912984849674963 2314 70-71 -2.515770752385567 2314 72-73 -2.5197063024005892 2314 74-75 -1.9720004780660716 2314 76-77 -0.8125629688897718 2314 78-79 -0.3700997928749672 2314 80-81 -0.13591124575211566 2314 82-83 -0.3681514035321918 2314 84-85 -0.011196103041282157 2314 86-87 0.04992403668170553 2314 88-89 0.2465304799344885 2314 90-91 0.7066597919979891 2314 92-93 1.2558024765008966 2314 94-95 1.2295893052990614 2314 96-97 0.9412453232639599 2314 98-99 0.9971132843674333 2314 100-101 1.0358052461552631 2315 1 -14.195980998276237 2315 2 -7.378656864605935 2315 3 -4.745241447373388 2315 4 -2.944783561167558 2315 5 -3.7304805685090727 2315 6 -3.008232183609657 2315 7 -3.0644475132302773 2315 8 -3.536387546160366 2315 9 -2.3362186862031322 2315 10-11 -4.625670867425381 2315 12-13 -4.305067758228322 2315 14-15 -3.424058821024147 2315 16-17 -4.641377280231005 2315 18-19 -1.551501127930635 2315 20-21 -3.42327773533264 2315 22-23 -4.447382226141848 2315 24-25 -4.950522419730387 2315 26-27 -4.9954362919552935 2315 28-29 -2.516262425781669 2315 30-31 -3.281284824534662 2315 32-33 -4.468867332128134 2315 34-35 -3.9228105587610003 2315 36-37 -5.400120490920653 2315 38-39 -3.7239909900227843 2315 40-41 -4.62835749899066 2315 42-43 -3.30436004072277 2315 44-45 -1.9761299790991629 2315 46-47 -1.421292481912225 2315 48-49 -1.1204983788642338 2315 50-51 -0.9834893756895369 2315 52-53 -0.8444046800942608 2315 54-55 -2.1746365521363984 2315 56-57 -4.19137777170323 2315 58-59 -3.482571225755258 2315 60-61 -4.7515574070343405 2315 62-63 -2.59476137307837 2315 64-65 -3.534039274521998 2315 66-67 -1.9081777783797769 2315 68-69 -1.5124851992010981 2315 70-71 -2.0361984675978952 2315 72-73 -2.192844686918626 2315 74-75 -2.9275242188306443 2315 76-77 -2.4503420781956784 2315 78-79 -1.5012988066710165 2315 80-81 -1.2334661999371797 2315 82-83 -0.9633939190071139 2315 84-85 -0.9190520098692403 2315 86-87 -1.3850827655716351 2315 88-89 -1.9610603763963574 2315 90-91 -1.4534750743389573 2315 92-93 -0.5557014354086931 2315 94-95 -0.3782553053681177 2315 96-97 -1.425298395333943 2315 98-99 -1.0313813630531943 2315 100-101 -0.738072463900119 2316 1 -14.195980998276237 2316 2 -7.379909544336005 2316 3 -5.102995116603196 2316 4 -2.946314050804876 2316 5 -2.323759681376476 2316 6 -1.2809130650592948 2316 7 -4.879316469874503 2316 8 -2.6966352723179625 2316 9 -1.208855625097101 2316 10-11 -4.511904699918681 2316 12-13 -3.798696879889519 2316 14-15 -1.8885237649524953 2316 16-17 -3.2346590866925666 2316 18-19 -2.3217628449331755 2316 20-21 -3.140302148274351 2316 22-23 -2.8358729736459125 2316 24-25 -3.503519624202177 2316 26-27 -4.382308242186127 2316 28-29 -3.8483025698414224 2316 30-31 -3.004941236001766 2316 32-33 -2.937931561673061 2316 34-35 -4.290040926024638 2316 36-37 -2.6093168438997942 2316 38-39 -3.3689339254383164 2316 40-41 -2.97661387211366 2316 42-43 -6.762180944175377 2316 44-45 -3.590096000729254 2316 46-47 -4.8828177111186015 2316 48-49 -4.428562101907829 2316 50-51 -3.476824684325255 2316 52-53 -4.751215089620924 2316 54-55 -4.887581384687266 2316 56-57 -4.9726665878474705 2316 58-59 -3.6586028327059985 2316 60-61 -3.432597356204635 2316 62-63 -2.34355361299205 2316 64-65 -2.6144636053883197 2316 66-67 -2.990771456038946 2316 68-69 -2.8792106495990915 2316 70-71 -3.180884930597152 2316 72-73 -3.9593138761626285 2316 74-75 -4.695571642988799 2316 76-77 -4.484194958308027 2316 78-79 -4.247035031973795 2316 80-81 -4.233865960999534 2316 82-83 -3.815681387826121 2316 84-85 -3.782953041938324 2316 86-87 -3.917889580470483 2316 88-89 -3.6105280218411977 2316 90-91 -2.8494056345378844 2316 92-93 -2.7414004574227526 2316 94-95 -2.0586481053381185 2316 96-97 -2.0722152599269634 2316 98-99 -2.138162618384266 2316 100-101 -1.8933052458917015 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 16937.0 3 5224.0 4 5646.0 5 6698.0 6 8058.0 7 9841.0 8 12786.0 9 15469.0 10 19038.0 11 22825.0 12 26436.0 13 29872.0 14 32699.0 15 36442.0 16 40760.0 17 42636.0 18 38259.0 19 38398.0 20 41320.0 21 45583.0 22 50124.0 23 55961.0 24 62273.0 25 69358.0 26 77954.0 27 87885.0 28 98822.0 29 111260.0 30 126260.0 31 144320.0 32 165285.0 33 189779.0 34 218876.0 35 253054.0 36 263561.0 37 157161.0 38 20061.0 39 105.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.240517042146564 18.51690203111046 13.35992842837966 21.882652498363306 2 14.798031128675376 21.637809649556633 40.77153954001189 22.792619681756104 3 20.318467266120518 27.15232439346998 29.998135295824156 22.53107304458534 4 11.915347840734025 16.3061013600215 39.38630591210014 32.39224488714434 5 12.580563170603293 39.57663864732545 33.944819145137764 13.897979036933487 6 29.26530108831112 37.12875182829155 18.868082285793843 14.737864797603493 7 27.115113798627473 34.49323912732567 21.41716124316213 16.974485830884728 8 25.34352514038769 33.68207343216405 20.708820682865703 20.26558074458256 9 27.25803162751846 17.516459346181012 20.877937723274158 34.34757130302637 10-11 24.400294214382725 26.491985230271553 28.064621250047995 21.04309930529773 12-13 27.350974925620108 24.267450972826015 27.691659332238892 20.689914769314985 14-15 23.823279096695426 25.234441844632112 27.78008879213526 23.1621902665372 16-17 21.3397982479055 28.725924684489257 28.280495525752247 21.653781541852997 18-19 20.946043343751644 28.46281435116225 30.283609832836632 20.307532472249473 20-21 22.836985639604425 26.77310892885425 29.10281540653916 21.28709002500216 22-23 21.89999246760217 26.89467247506644 28.867365829636842 22.337969227694547 24-25 22.3071670494169 26.74274091825602 29.624224521394982 21.325867510932092 26-27 22.5581048893082 26.18136338950441 30.220202391528172 21.040329329659222 28-29 21.357329483429847 27.153460890726176 30.355942556583 21.13326706926097 30-31 21.510142734165875 27.45829891650166 29.769046415061407 21.26251193427106 32-33 21.435440392536954 27.352931154591715 29.486120365883206 21.72550808698813 34-35 22.340872341468557 26.63304008974658 29.342756194446633 21.683331374338223 36-37 22.49624761945496 26.352583914508365 30.029434257781872 21.121734208254804 38-39 22.45063103332772 26.37435444221347 30.314806999748306 20.860207524710507 40-41 21.837287864878466 26.730051960650176 30.37422379319748 21.05843638127388 42-43 21.220470407061274 27.61452530775457 29.281934083667917 21.88307020151625 44-45 21.74634463010051 27.432965564622286 28.709882250294065 22.110807554983143 46-47 22.06345134661602 27.30251793011208 27.983223264799616 22.65080745847228 48-49 22.35478000303286 27.193973049943192 28.322625238515975 22.12862170850797 50-51 22.36036969422257 27.671514860967054 28.57159086132604 21.39652458348433 52-53 22.071945383674684 27.941435214514758 28.506431683286827 21.48018771852373 54-55 21.112824247712737 27.21457622971836 29.08750668937551 22.58509283319339 56-57 22.093473662847185 28.143309673300713 27.70719062544727 22.05602603840483 58-59 22.31918356672371 27.36097583222613 27.97261196313417 22.34722863791599 60-61 21.999673340104998 27.041118077710607 28.901641115844463 22.05756746633993 62-63 22.708961262024292 26.8881770392206 28.434118944086155 21.968742754668952 64-65 22.18180813468716 27.376534436902865 28.64267079167917 21.798986636730806 66-67 21.562155424426262 27.47400766966319 28.387368334988118 22.57646857092243 68-69 21.389203870207847 28.071610351034693 28.320557035000522 22.218628743756934 70-71 21.76862224875316 27.80955034286548 27.689593321471378 22.732234086909976 72-73 21.76277531651141 27.128613646048255 27.844182435209312 23.26442860223102 74-75 21.513704085377423 28.02981498755689 27.818920269033264 22.637560658032424 76-77 22.202418769179204 26.83023452005715 28.105745641983155 22.861601068780494 78-79 21.662260365978586 27.08562690089175 28.445691160548026 22.806421572581637 80-81 21.681630934089178 27.958063945590084 27.908696444214343 22.451608676106392 82-83 21.646584786496494 27.604971886630082 27.78144375574551 22.966999571127914 84-85 21.574293472932 27.18927705976618 27.671545124186203 23.564884343115615 86-87 21.895337419062077 27.17486680490219 28.002973710828773 22.92682206520696 88-89 22.18539805409114 26.58252051859855 27.94325199222515 23.288829435085162 90-91 21.483375668604832 26.80438833057061 28.45007854693006 23.262157453894496 92-93 21.406795150582706 27.38459843602355 28.267023084098565 22.94158332929518 94-95 21.307168180370418 27.41325002376504 27.66851118736965 23.611070608494895 96-97 20.99396602212812 26.926739901762232 27.642403468855388 24.43689060725426 98-99 21.427660713186224 26.99354447586164 27.17867545716762 24.40011935378451 100-101 21.74144494615528 26.798860683835972 27.59251734122936 23.867177028779384 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3803.0 1 2620.5 2 1302.5 3 1021.5 4 868.0 5 919.0 6 1213.5 7 2460.5 8 3375.5 9 3041.5 10 2626.5 11 2481.5 12 2586.5 13 2808.5 14 3065.0 15 3308.5 16 3580.5 17 3879.5 18 4242.5 19 4692.0 20 5205.0 21 5902.0 22 6888.0 23 8241.0 24 10029.0 25 12582.0 26 16275.5 27 20502.5 28 24491.0 29 29135.5 30 34354.5 31 39653.5 32 45337.0 33 51660.5 34 58168.5 35 63164.0 36 68665.5 37 73333.0 38 75223.0 39 76550.5 40 77842.5 41 79226.0 42 81073.0 43 84544.0 44 88207.0 45 91573.5 46 100999.5 47 132037.5 48 157445.5 49 139465.5 50 108881.0 51 92622.0 52 83746.0 53 78234.5 54 72328.5 55 65000.5 56 57947.5 57 51232.0 58 45168.5 59 39288.5 60 33124.5 61 27131.5 62 21489.5 63 16354.0 64 12372.0 65 9134.0 66 6639.0 67 4864.5 68 3329.5 69 2276.0 70 1618.5 71 1198.0 72 924.5 73 675.0 74 503.5 75 390.0 76 266.5 77 163.5 78 92.5 79 54.5 80 43.0 81 29.5 82 18.5 83 16.0 84 15.0 85 13.0 86 13.0 87 9.0 88 6.0 89 6.5 90 5.5 91 5.5 92 4.5 93 4.0 94 4.5 95 3.5 96 2.5 97 2.0 98 2.0 99 1.5 100 0.5 >>END_MODULE >>Per base N content fail #Base N-Count 1 53.08572715190557 2 0.08545439296780613 3 0.11994593177399844 4 0.19478463755172787 5 0.11854813666356129 6 0.1453706914854633 7 0.15201966282159676 8 0.15065964595738765 9 0.13959061981257456 10-11 0.12752047014271867 12-13 0.1386650527799878 14-15 0.1468251539652425 16-17 0.14100730404612574 18-19 0.14820405995256564 20-21 0.17583884706837033 22-23 0.19285794699409828 24-25 0.2204171776174469 26-27 0.2138437627737695 28-29 0.21059483359815884 30-31 0.22657503175261595 32-33 0.21707380282626615 34-35 0.20269918013650035 36-37 0.19027013712747817 38-39 0.18628453215042087 40-41 0.1862656430273069 42-43 0.18067446258555828 44-45 0.19620132178527905 46-47 0.22170163798919995 48-49 0.22498834541103868 50-51 0.23607626067896573 52-53 0.2648066169353833 54-55 0.25103644618526605 56-57 0.22682059035309815 58-59 0.1832433833290644 60-61 0.1939724052578252 62-63 0.1979013428655404 64-65 0.18636008864287695 66-67 0.19603131967725287 68-69 0.18488673703998373 70-71 0.19820356883536466 72-73 0.21799936985885293 74-75 0.24967642932105688 76-77 0.25060199635364366 78-79 0.2451430397736932 80-81 0.2502808812607054 82-83 0.24096854356549577 84-85 0.20963148831934403 86-87 0.22219275519016435 88-89 0.22464834119498636 90-91 0.21199262870859598 92-93 0.2098203795504842 94-95 0.2490719773814084 96-97 0.2760834234344506 98-99 0.2951803269027203 100-101 0.29178028474219747 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2647026.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.9890508863628 #Duplication Level Percentage of deduplicated Percentage of total 1 81.25570237235597 54.432423810289045 2 11.38434958183149 15.25253546890905 3 3.555645001916844 7.14567851901747 4 1.4769475173251614 3.9575724957832974 5 0.7414110648513833 2.483321177552087 6 0.41752451861573536 1.678174273431218 7 0.27322207210727417 1.2812021104170175 8 0.17407083536860332 0.9328672038671237 9 0.12539909844661146 0.7560329928249665 >10 0.5555566560431774 6.217694748455725 >50 0.025794286276744044 1.194302767342606 >100 0.011426693113010741 1.3849016473884124 >500 0.0013112452213180485 0.5721398010433856 >1k 0.001507932004514297 2.1258373217072966 >5k 1.31124522131678E-4 0.5853156619712236 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 7614 0.28764356678022807 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 5713 0.21582712070074114 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4952 0.18707787532120954 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4889 0.18469784580884357 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3307 0.12493266027609852 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 2747 0.10377684238840118 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 2653 0.10022568724296625 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 3.7778246228031E-5 5 0.0 0.0 0.0 0.0 3.7778246228031E-5 6 0.0 0.0 0.0 0.0 3.7778246228031E-5 7 0.0 0.0 0.0 0.0 3.7778246228031E-5 8 0.0 0.0 0.0 0.0 7.5556492456062E-5 9 0.0 0.0 0.0 0.0 1.1333473868409302E-4 10-11 1.88891231140155E-5 0.0 0.0 0.0 1.1333473868409302E-4 12-13 3.7778246228031E-5 0.0 0.0 3.7778246228031E-5 1.1333473868409302E-4 14-15 5.666736934204651E-5 0.0 0.0 5.666736934204651E-5 1.1333473868409302E-4 16-17 7.5556492456062E-5 0.0 0.0 1.3222386179810851E-4 1.1333473868409302E-4 18-19 7.5556492456062E-5 0.0 0.0 2.2666947736818603E-4 1.1333473868409302E-4 20-21 7.5556492456062E-5 0.0 0.0 2.2666947736818603E-4 1.1333473868409302E-4 22-23 7.5556492456062E-5 0.0 0.0 5.666736934204651E-4 1.8889123114015503E-4 24-25 7.5556492456062E-5 0.0 0.0 0.0011522365099549457 2.455586004822015E-4 26-27 7.5556492456062E-5 0.0 0.0 0.0019833579269716278 2.6444772359621703E-4 28-29 7.5556492456062E-5 0.0 0.0 0.0054211783337224494 2.8333684671023255E-4 30-31 7.5556492456062E-5 0.0 0.0 0.014771294275160121 3.02225969824248E-4 32-33 7.5556492456062E-5 0.0 0.0 0.033358191419351375 3.02225969824248E-4 34-35 7.5556492456062E-5 0.0 0.0 0.06386412524848642 3.02225969824248E-4 36-37 7.5556492456062E-5 0.0 0.0 0.10868801439804521 3.02225969824248E-4 38-39 1.1333473868409302E-4 0.0 0.0 0.16909543011666678 3.400042160522791E-4 40-41 1.1333473868409302E-4 0.0 0.0 0.23887185089984003 3.400042160522791E-4 42-43 1.1333473868409302E-4 0.0 0.0 0.3084027130825311 3.400042160522791E-4 44-45 1.1333473868409302E-4 0.0 0.0 0.39349821271117097 3.400042160522791E-4 46-47 1.1333473868409302E-4 0.0 0.0 0.4955183666499687 4.1556070850834107E-4 48-49 1.1333473868409302E-4 0.0 0.0 0.6070019712688881 4.5333895473637206E-4 50-51 1.1333473868409302E-4 0.0 0.0 0.7270234595353426 4.5333895473637206E-4 52-53 1.1333473868409302E-4 0.0 0.0 0.841925995437899 4.91117200964403E-4 54-55 1.1333473868409302E-4 0.0 0.0 0.9670853251913658 5.288954471924341E-4 56-57 1.1333473868409302E-4 0.0 0.0 1.1140427030184064 5.288954471924341E-4 58-59 1.1333473868409302E-4 0.0 0.0 1.2887859809461637 5.288954471924341E-4 60-61 1.1333473868409302E-4 0.0 0.0 1.5025730763505911 5.288954471924341E-4 62-63 1.1333473868409302E-4 0.0 0.0 1.7078600663537116 5.288954471924341E-4 64-65 1.1333473868409302E-4 0.0 0.0 1.8999246701770214 5.477845703064496E-4 66-67 1.1333473868409302E-4 0.0 0.0 2.111596183792679 6.044519396484962E-4 68-69 1.1333473868409302E-4 0.0 0.0 2.355568097933303 6.422301858765271E-4 70-71 1.1333473868409302E-4 0.0 0.0 2.6407183004624812 6.422301858765271E-4 72-73 1.1333473868409302E-4 0.0 0.0 2.9395442281262065 6.800084321045581E-4 74-75 1.1333473868409302E-4 3.7778246228031E-5 0.0 3.242676875859927 6.800084321045581E-4 76-77 1.1333473868409302E-4 3.7778246228031E-5 0.0 3.5443739502369827 6.800084321045581E-4 78-79 1.1333473868409302E-4 3.7778246228031E-5 0.0 3.8950316317255664 6.800084321045581E-4 80-81 1.1333473868409302E-4 3.7778246228031E-5 0.0 4.2872076058187565 6.988975552185736E-4 82-83 1.1333473868409302E-4 3.7778246228031E-5 0.0 4.688072576544394 7.744540476746357E-4 84-85 1.1333473868409302E-4 3.7778246228031E-5 0.0 5.126035785065957 8.311214170166821E-4 86-87 1.1333473868409302E-4 3.7778246228031E-5 0.0 5.588932636098021 8.311214170166821E-4 88-89 1.1333473868409302E-4 3.7778246228031E-5 0.0 6.0476927691681155 8.688996632447131E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 13960 0.0 32.236214 1 TACATGG 16310 0.0 26.273827 2 ACATGGG 16220 0.0 25.691885 3 CATGGGA 11925 0.0 23.847923 4 GAGTACT 9185 0.0 23.278637 12-13 ATGGGAG 4270 0.0 22.660027 5 GTATAGG 655 1.0913936E-11 21.518335 1 AGTACTT 9815 0.0 21.207367 12-13 GTACTTT 10270 0.0 20.96075 14-15 CATGGGG 4615 0.0 20.779581 4 GGTCGGG 700 2.7284841E-11 20.135014 1 ATGGGAT 4800 0.0 19.961317 5 GCCTACG 380 1.1965962E-4 18.545406 1 GCTTATA 2315 0.0 18.265022 1 ACTTTTT 13125 0.0 17.608873 16-17 GTCGGGA 565 0.0 17.560661 2 GTATAGT 805 2.055458E-10 17.508707 1 GTATATA 1640 0.0 17.188427 1 GTGTAGC 1325 0.0 16.71586 1 TACTTTT 12465 0.0 16.5312 14-15 >>END_MODULE