FastQCFastQC Report
Fri 27 May 2016
SRR939646_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939646_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2647026
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA175530.6631215560406283No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA151350.5717737566612493No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT125350.47355031646836865No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT124320.46965915710688144No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT93940.3548888450661233No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91090.34412204489113446No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA70560.2665633053849868No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG68240.2577987522600836No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT67300.25424759711464867No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66830.2524720195419312No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT63010.23804072948282337No Hit
TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC54590.20623144615882125No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT53710.20290696049075452No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC49620.18745565778348985No Hit
GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG45630.17238213753850548No Hit
TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG36710.13868394190310182No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT35720.13494389552652675No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT32880.12421487359776595No Hit
ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG31290.11820813244750902No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC30720.11605477241251126No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC30090.11367474290014529No Hit
CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG29410.11110582215663918No Hit
GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT29050.10974580529243007No Hit
ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA28420.10736577578006412No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG166900.026.4610541
TACATGG166700.025.9223042
ACATGGG165850.024.6865583
CATGGGA123200.022.1295914
CATGGGG43150.021.7534714
ATGGGAG43350.021.3266815
AGTACTT103650.020.78415512-13
GAGTACT101450.019.95706212-13
ATGGGAT45950.019.093425
TACCTGG15650.018.9906562
GTACTTT110700.018.3533414-15
TTACGCG1405.7495527E-717.31991862-63
GTCGGGA4650.017.2468472
GCGTTTA12350.017.08150562-63
ATGGGGA23250.016.6359335
TACTTTT129700.016.5733514-15
AGTACAT123850.016.4551372
ACTTTTT132450.015.78436716-17
GCTTATA28100.015.7837121
CTTTGCG6000.015.7250662
Produced by FastQC (version 0.11.5)