##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939646_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2647026 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.501400817370136 31.0 31.0 34.0 30.0 34.0 2 31.688359691215727 31.0 31.0 34.0 30.0 34.0 3 31.747645093021376 31.0 31.0 34.0 30.0 34.0 4 35.52465143901118 37.0 35.0 37.0 33.0 37.0 5 35.29394686716338 37.0 35.0 37.0 33.0 37.0 6 35.3135469768714 37.0 35.0 37.0 32.0 37.0 7 35.22966189225191 37.0 35.0 37.0 32.0 37.0 8 35.196576081987864 37.0 35.0 37.0 32.0 37.0 9 36.780853682585665 39.0 35.0 39.0 32.0 39.0 10-11 36.660461778614945 39.0 35.0 39.0 32.0 39.0 12-13 36.601395868419885 39.0 35.0 39.0 32.0 39.0 14-15 37.60814986328053 39.0 36.0 41.0 32.0 41.0 16-17 37.58151374410376 39.0 36.0 41.0 32.0 41.0 18-19 37.613544974624354 39.0 36.0 41.0 32.0 41.0 20-21 37.56241854065657 39.0 36.0 41.0 32.0 41.0 22-23 37.42924814489922 39.0 36.0 41.0 32.0 41.0 24-25 37.28890573798671 39.0 36.0 41.0 31.0 41.0 26-27 36.962970140829746 39.0 36.0 40.0 30.5 41.0 28-29 36.767917277729794 39.0 35.5 40.0 30.0 41.0 30-31 36.547645357469094 38.0 35.0 40.0 30.0 41.0 32-33 36.38432867678671 38.0 35.0 40.0 30.0 41.0 34-35 36.346182848222874 38.0 35.0 40.0 30.0 41.0 36-37 36.23494744668167 38.0 35.0 40.0 30.0 41.0 38-39 36.246414088868036 38.0 35.0 40.0 30.0 41.0 40-41 36.13443332252876 38.0 35.0 40.0 30.0 41.0 42-43 35.96917408442532 38.0 35.0 40.0 29.5 41.0 44-45 35.752303717454986 38.0 35.0 40.0 28.5 41.0 46-47 35.423364560831665 38.0 34.5 40.0 27.0 41.0 48-49 35.09935112084278 38.0 34.0 40.0 26.0 41.0 50-51 34.85910017506439 38.0 34.0 40.0 26.0 41.0 52-53 34.594241613040445 38.0 33.0 40.0 24.0 41.0 54-55 34.33914287203828 38.0 33.0 40.0 24.0 41.0 56-57 33.587272093285065 37.5 32.5 40.0 21.0 41.0 58-59 30.59978405954456 35.5 27.0 39.0 8.0 40.5 60-61 30.323424666021417 34.5 26.0 39.0 10.5 40.0 62-63 32.085558283144934 35.5 30.5 39.0 18.0 40.0 64-65 32.1046897537085 36.0 31.0 39.0 15.0 40.0 66-67 27.282388046056216 32.0 18.5 37.0 2.0 39.5 68-69 19.904236490310257 20.5 8.0 32.5 2.0 37.0 70-71 17.966737009761143 16.0 7.5 29.0 2.0 35.5 72-73 19.696931008611173 20.0 7.5 31.0 2.0 35.5 74-75 21.11306877983065 24.0 9.0 32.5 2.0 36.0 76-77 22.845091245798116 27.5 11.5 33.0 2.0 35.5 78-79 24.986077205135125 30.5 13.0 34.5 2.0 36.5 80-81 26.582383777114394 32.0 22.5 35.0 2.0 37.0 82-83 27.71114866268786 33.0 26.5 35.0 2.0 36.0 84-85 27.761206916743546 34.0 27.0 35.0 2.0 36.0 86-87 27.486966693942563 34.0 26.5 35.0 2.0 36.0 88-89 27.029465332036786 33.0 26.0 35.0 2.0 35.0 90-91 25.22252369262712 32.5 16.0 34.5 2.0 35.0 92-93 23.67239611548961 31.5 2.0 34.0 2.0 35.0 94-95 20.02414218825202 28.5 2.0 34.0 2.0 35.0 96-97 17.336843876864073 18.5 2.0 33.0 2.0 35.0 98-99 17.627311934223542 22.0 2.0 33.0 2.0 35.0 100-101 16.854935690091445 18.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.2689198170944387 1101 2 0.24417806735827696 1101 3 0.2513176785634421 1101 4 0.21243874593769618 1101 5 0.3116091543631967 1101 6 0.3425120503342498 1101 7 0.32213573177453725 1101 8 0.37597763065260636 1101 9 0.3815562294696946 1101 10-11 0.3072338427460011 1101 12-13 0.3477502359959601 1101 14-15 0.5264573547896774 1101 16-17 0.44713973722270595 1101 18-19 0.5204030825525265 1101 20-21 0.5148687641354002 1101 22-23 0.4635196800275736 1101 24-25 0.3574182655023961 1101 26-27 0.47438073286820526 1101 28-29 0.42697354297826706 1101 30-31 0.2294437505393816 1101 32-33 0.2638849669856569 1101 34-35 0.21135017729161376 1101 36-37 -0.031058950541698493 1101 38-39 0.06343019825352059 1101 40-41 0.1324761965398693 1101 42-43 0.24104344708884895 1101 44-45 0.19724443785504064 1101 46-47 0.03666373741938145 1101 48-49 0.12186476529728907 1101 50-51 0.2426246344421159 1101 52-53 0.16568685525506766 1101 54-55 0.10913094150029679 1101 56-57 -3.326339478750157 1101 58-59 -9.58056769678722 1101 60-61 -11.696375863983231 1101 62-63 -3.9094870931803385 1101 64-65 -1.1751559853737916 1101 66-67 -2.2595759323455695 1101 68-69 -4.646652247419421 1101 70-71 -4.504042823124834 1101 72-73 -3.52330636370875 1101 74-75 -6.06493873957141 1101 76-77 -4.502343977988808 1101 78-79 1.3725384032073826 1101 80-81 1.8815855274353588 1101 82-83 0.5455516194750594 1101 84-85 0.19568846867321454 1101 86-87 0.1653715070737718 1101 88-89 0.029985756956172338 1101 90-91 1.4707759113830328 1101 92-93 1.2681348919391269 1101 94-95 -8.289602894558573 1101 96-97 -9.36118187981509 1101 98-99 -8.320758462496045 1101 100-101 -9.418122441396696 1102 1 0.2580961029208986 1102 2 0.29860804099672933 1102 3 0.2884562729882525 1102 4 0.2321033373337329 1102 5 0.3078467448057367 1102 6 0.31059730469512203 1102 7 0.205312578484957 1102 8 0.26684711295671804 1102 9 0.3399794725007155 1102 10-11 0.3326234888282258 1102 12-13 0.30525475111178935 1102 14-15 0.4244902225893341 1102 16-17 0.3246684825253041 1102 18-19 0.3151532087514184 1102 20-21 0.3017128944848295 1102 22-23 0.3027546343155336 1102 24-25 0.20899423723384558 1102 26-27 0.09779202907102302 1102 28-29 0.2836647763620803 1102 30-31 0.17773742937722403 1102 32-33 0.07573689362202884 1102 34-35 0.14276977457694073 1102 36-37 -0.07050973297481278 1102 38-39 0.12782438749576386 1102 40-41 -0.04135834269396099 1102 42-43 0.13917660093931516 1102 44-45 -0.036127708647846646 1102 46-47 -0.012992440717368936 1102 48-49 0.22840377477622553 1102 50-51 0.21460354520547753 1102 52-53 0.06428195312932417 1102 54-55 -0.030748356272994215 1102 56-57 -3.290175924989427 1102 58-59 -10.16663960211001 1102 60-61 -8.175959015029278 1102 62-63 -2.7818072457407723 1102 64-65 -0.8995661037203071 1102 66-67 -2.0314700374552253 1102 68-69 -5.253020075115872 1102 70-71 -5.033234140641239 1102 72-73 -3.8498263031332804 1102 74-75 -4.534534790948381 1102 76-77 -4.452395691489283 1102 78-79 1.4944092877213784 1102 80-81 1.7607720213497657 1102 82-83 0.5187130979651613 1102 84-85 0.14849255190839372 1102 86-87 -0.030351864960550756 1102 88-89 -0.16191732229672695 1102 90-91 1.281559017558365 1102 92-93 0.8475980138192121 1102 94-95 -8.633572831178686 1102 96-97 -9.47529372007534 1102 98-99 -8.343892233318861 1102 100-101 -9.265633126236002 1103 1 0.14348395917864565 1103 2 0.1781102537777528 1103 3 0.24960621103038605 1103 4 0.1994780939278229 1103 5 0.11101190678130735 1103 6 0.25233000074752 1103 7 0.18915750679084908 1103 8 0.3369352701526793 1103 9 0.3612632395391273 1103 10-11 0.2633135224624965 1103 12-13 0.32479130625429065 1103 14-15 0.3714511006208383 1103 16-17 0.322984998722859 1103 18-19 0.23201460014812625 1103 20-21 0.301090837483585 1103 22-23 0.2563269479993693 1103 24-25 0.31960885929084526 1103 26-27 0.34990744772919413 1103 28-29 0.31529196701529116 1103 30-31 0.35143755329296056 1103 32-33 0.3229137390814003 1103 34-35 0.19658814951055348 1103 36-37 0.2782259724597722 1103 38-39 0.3548107567646639 1103 40-41 0.29099711844960297 1103 42-43 0.33376927220856345 1103 44-45 0.26554550785065345 1103 46-47 0.28128442244425145 1103 48-49 0.364604164225689 1103 50-51 -0.004501075081300598 1103 52-53 0.1441523726726004 1103 54-55 0.22527005406512046 1103 56-57 0.5777635333217788 1103 58-59 -8.23950984463081 1103 60-61 -7.298356253478563 1103 62-63 -1.9363609720640191 1103 64-65 -0.4143035793797125 1103 66-67 -1.880018453104011 1103 68-69 -3.3045438846396813 1103 70-71 -3.715529768185938 1103 72-73 -2.8084837646308927 1103 74-75 -3.890098961598053 1103 76-77 -0.4592053923212909 1103 78-79 3.345495143625076 1103 80-81 2.876970286529442 1103 82-83 1.2133358630563293 1103 84-85 0.7098283635564719 1103 86-87 0.7875826367211616 1103 88-89 0.563864231960661 1103 90-91 1.7224402354730373 1103 92-93 1.0598323915851893 1103 94-95 -7.000205863573802 1103 96-97 -8.940331779908938 1103 98-99 -8.246191401313196 1103 100-101 -9.65655930786107 1104 1 0.2194045004273164 1104 2 0.2858513748539231 1104 3 0.2631273400949645 1104 4 0.18382422370808627 1104 5 0.26988142446418095 1104 6 0.18479333382184393 1104 7 0.1555163607679475 1104 8 0.29547134680867515 1104 9 0.31582692391251754 1104 10-11 0.30565235361947174 1104 12-13 0.2387259648998281 1104 14-15 0.35513886840629283 1104 16-17 0.26422215217797884 1104 18-19 0.38534546607238696 1104 20-21 0.20120977750646318 1104 22-23 0.27907502825969743 1104 24-25 0.38368290610073785 1104 26-27 0.4613102856585911 1104 28-29 0.2942956108741086 1104 30-31 0.3449306820358302 1104 32-33 0.3790252796034821 1104 34-35 0.2703835835768871 1104 36-37 0.23266424156549448 1104 38-39 0.15283648870588706 1104 40-41 0.2536301847302056 1104 42-43 0.1463348596223213 1104 44-45 0.07785269891672186 1104 46-47 -0.0737541359345073 1104 48-49 -0.03412668779383665 1104 50-51 0.04111661556012791 1104 52-53 0.04956499571930095 1104 54-55 0.053772991099840795 1104 56-57 0.28768776614158753 1104 58-59 -8.165957743447951 1104 60-61 -7.622095114353495 1104 62-63 -2.3568852487791716 1104 64-65 -0.533123230861591 1104 66-67 -1.7368448606518285 1104 68-69 -3.4157833419187433 1104 70-71 -3.8208693232071163 1104 72-73 -3.0569569019074443 1104 74-75 -3.7204403910321986 1104 76-77 -0.26625959216506345 1104 78-79 3.447750164666214 1104 80-81 2.8555619003376655 1104 82-83 1.3354014525149047 1104 84-85 0.9214034429975015 1104 86-87 0.8227798863098101 1104 88-89 0.702142607505774 1104 90-91 2.1267142378422967 1104 92-93 1.8806344222219593 1104 94-95 -6.034183654165748 1104 96-97 -7.717344115591082 1104 98-99 -5.700572415622805 1104 100-101 -5.077242534795905 1105 1 0.1466175671067198 1105 2 0.18951181264767314 1105 3 0.21583946646995145 1105 4 0.12426295929999753 1105 5 0.21289912402082933 1105 6 0.21564190086266422 1105 7 0.18073969059211237 1105 8 0.21360606433004392 1105 9 0.18376689801451818 1105 10-11 0.11300694290303426 1105 12-13 0.2247517716638896 1105 14-15 0.27117544348542566 1105 16-17 0.30744953219434734 1105 18-19 0.3212875705588871 1105 20-21 0.19528847517314318 1105 22-23 0.3171918400624776 1105 24-25 0.3125116882857739 1105 26-27 0.21302282770204073 1105 28-29 0.3430672884796664 1105 30-31 0.2945638917388962 1105 32-33 0.1979329638779319 1105 34-35 0.3531028125714144 1105 36-37 0.3649068164249556 1105 38-39 0.448217431397012 1105 40-41 0.3730900511838371 1105 42-43 0.190057509888085 1105 44-45 0.250506088541222 1105 46-47 0.3320481249434053 1105 48-49 0.23685644238102554 1105 50-51 0.33000272173124756 1105 52-53 0.5197853668990646 1105 54-55 0.3040507611850103 1105 56-57 0.6588114101854288 1105 58-59 -8.114866978284851 1105 60-61 -7.16129471107347 1105 62-63 -1.8189719232133683 1105 64-65 -0.12329793603732497 1105 66-67 -1.591529800141764 1105 68-69 -3.2489254971898447 1105 70-71 -3.6946043739589385 1105 72-73 -2.6661200436867816 1105 74-75 -3.179354165177461 1105 76-77 0.110894522370792 1105 78-79 3.8377333439399983 1105 80-81 3.2635335439084407 1105 82-83 1.6534594713777153 1105 84-85 1.2124682298848022 1105 86-87 1.093686424853729 1105 88-89 0.9991545840287337 1105 90-91 2.3619455987504914 1105 92-93 2.1297781010310395 1105 94-95 -5.868058680972819 1105 96-97 -7.5264568339892755 1105 98-99 -5.229798176579054 1105 100-101 -4.7309636104180015 1106 1 0.028820656707683412 1106 2 0.07630854406336951 1106 3 0.15870325803408036 1106 4 0.0824823601440201 1106 5 0.15496065838853923 1106 6 0.10857277158230971 1106 7 0.1470161137340753 1106 8 0.15019712329370094 1106 9 0.0780470074741828 1106 10-11 0.08528023198859103 1106 12-13 0.08405311097671131 1106 14-15 -0.00878294948702063 1106 16-17 0.20413819515709264 1106 18-19 0.17223404397986286 1106 20-21 0.05226619887460515 1106 22-23 0.10077992547396519 1106 24-25 0.21069418899458014 1106 26-27 0.09903591302113313 1106 28-29 0.16857918612019773 1106 30-31 0.2910220410511286 1106 32-33 0.19068955022736134 1106 34-35 0.38905569560236586 1106 36-37 0.3610791995591214 1106 38-39 0.26940277630426124 1106 40-41 0.2796732195691476 1106 42-43 0.287181976171901 1106 44-45 0.28158147748681017 1106 46-47 0.2449673828647434 1106 48-49 0.2042647767747141 1106 50-51 0.2447025735288193 1106 52-53 0.24151867010265704 1106 54-55 0.36027548770744033 1106 56-57 0.8612030289076174 1106 58-59 -8.110293795480558 1106 60-61 -7.335321429749143 1106 62-63 -1.762171176551881 1106 64-65 -0.07839271801291403 1106 66-67 0.8959832738258733 1106 68-69 -2.34043389911146 1106 70-71 -2.601210641227002 1106 72-73 -1.121860208446492 1106 74-75 -1.0918652988389468 1106 76-77 2.199197789318376 1106 78-79 4.418287793682371 1106 80-81 3.4843287024882095 1106 82-83 1.9145825073787108 1106 84-85 1.5328857019325106 1106 86-87 1.3778379910107788 1106 88-89 1.1370083203192145 1106 90-91 2.5585149130858937 1106 92-93 2.4392297414740938 1106 94-95 -6.047172169611601 1106 96-97 -7.729704167322257 1106 98-99 -5.596297787725863 1106 100-101 -4.876496471284586 1107 1 0.04260838114907628 1107 2 0.00896579650640561 1107 3 0.022436298837153146 1107 4 0.05319536949379966 1107 5 0.055803842343379984 1107 6 -0.007483431159315046 1107 7 0.12341721618071233 1107 8 0.018387875331455916 1107 9 0.0407018318004404 1107 10-11 0.07818576957415502 1107 12-13 0.02206452669632597 1107 14-15 0.08879080838587328 1107 16-17 0.11765100933934036 1107 18-19 -0.025327177975007942 1107 20-21 0.1234029426577763 1107 22-23 0.1910695880728568 1107 24-25 0.14215931771792611 1107 26-27 0.10582533051286447 1107 28-29 0.06928802023136171 1107 30-31 0.03506888279046194 1107 32-33 -0.029810616323082684 1107 34-35 -0.047843045806764906 1107 36-37 0.039085983492945786 1107 38-39 -0.019873415347611 1107 40-41 -0.02381721104919876 1107 42-43 -0.1862142042371815 1107 44-45 -0.13427471168592575 1107 46-47 -0.1404634446282742 1107 48-49 -0.25958810128332743 1107 50-51 -0.16171207995189008 1107 52-53 -0.11826460007073791 1107 54-55 -0.04088810819067845 1107 56-57 0.4622305405929268 1107 58-59 -8.61579467873354 1107 60-61 -7.86727539551168 1107 62-63 -2.4409970673162142 1107 64-65 -0.596358422903883 1107 66-67 0.36373784478120896 1107 68-69 -2.2627215370894547 1107 70-71 -2.5395574533290226 1107 72-73 -1.089782796410887 1107 74-75 -0.9836231304082403 1107 76-77 1.9966624349689113 1107 78-79 3.986468840904653 1107 80-81 2.9019018533319034 1107 82-83 1.354351884745295 1107 84-85 0.8367552707428452 1107 86-87 0.6650939614489673 1107 88-89 0.5983126322159187 1107 90-91 1.906879594290995 1107 92-93 1.843807557770738 1107 94-95 -6.681060620090516 1107 96-97 -8.09582681170466 1107 98-99 -5.943380693630726 1107 100-101 -5.102280087311586 1108 1 -0.05398818560520979 1108 2 -0.04437599777334711 1108 3 -0.07299237816376802 1108 4 0.04753859097669988 1108 5 -0.010390738447377146 1108 6 -0.02841313212983465 1108 7 -0.038180697950316755 1108 8 0.030357061847702482 1108 9 -0.013613673267492743 1108 10-11 -0.07796901187472827 1108 12-13 -0.0876298152885866 1108 14-15 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0.723409464471402 2314 78-79 3.3618691937176735 2314 80-81 2.4903379901101523 2314 82-83 0.9517372635636043 2314 84-85 0.5748720799908114 2314 86-87 0.6999450538069532 2314 88-89 0.8889624194304702 2314 90-91 2.162176973215203 2314 92-93 3.728675922948966 2314 94-95 6.454500092906201 2314 96-97 3.7509086252695774 2314 98-99 6.151647053582341 2314 100-101 6.382626665816412 2315 1 -0.44749338804790284 2315 2 -0.42072200826435235 2315 3 -0.5105979142038635 2315 4 -0.30352884657430934 2315 5 -0.48085604665567416 2315 6 -0.5364174736300029 2315 7 -0.5555412802999342 2315 8 -0.7041742335129086 2315 9 -0.7304441728194817 2315 10-11 -0.5905489249648781 2315 12-13 -0.5446762833709755 2315 14-15 -0.7134211074599293 2315 16-17 -0.7072855404632108 2315 18-19 -0.7094773331205104 2315 20-21 -0.7544841498501427 2315 22-23 -0.7903816546828182 2315 24-25 -0.5750911486446384 2315 26-27 -0.6999091203542633 2315 28-29 -0.6539509871847216 2315 30-31 -0.7115696313781044 2315 32-33 -0.7760989027654901 2315 34-35 -0.7402844126151749 2315 36-37 -0.620217450544601 2315 38-39 -0.7630853005173748 2315 40-41 -0.7630069469632588 2315 42-43 -0.7672864532478769 2315 44-45 -0.7620193342636057 2315 46-47 -0.7988380893216416 2315 48-49 -0.7871364551088291 2315 50-51 -0.637041774991566 2315 52-53 -0.5959676363616424 2315 54-55 -0.6821966321350601 2315 56-57 0.05718039684965248 2315 58-59 3.1673439775287413 2315 60-61 3.3556468963007724 2315 62-63 0.555657450482542 2315 64-65 -0.09617037301423181 2315 66-67 -1.803391219721231 2315 68-69 -5.224166216330978 2315 70-71 -3.737188163629746 2315 72-73 -2.9760923129855 2315 74-75 -0.6519600783047039 2315 76-77 1.6903169521888763 2315 78-79 3.4396375307427256 2315 80-81 2.3782826945845734 2315 82-83 0.703415889426573 2315 84-85 0.4461542924666837 2315 86-87 0.38851841418558664 2315 88-89 0.4783782442057323 2315 90-91 2.210143582418791 2315 92-93 3.8820676361509037 2315 94-95 6.389645559625908 2315 96-97 4.0488442109591425 2315 98-99 6.114222709209876 2315 100-101 6.514544213665475 2316 1 -0.38939097759802266 2316 2 -0.36832072188828846 2316 3 -0.49589796738914416 2316 4 -0.2831687605694313 2316 5 -0.505746969296176 2316 6 -0.5637164694475345 2316 7 -0.474902465396859 2316 8 -0.5275316032129282 2316 9 -0.7243567556541066 2316 10-11 -0.5729647725940694 2316 12-13 -0.5572545230385302 2316 14-15 -0.775907210362611 2316 16-17 -0.7639047840280213 2316 18-19 -0.7638662279071369 2316 20-21 -0.6488791501168691 2316 22-23 -0.8475236667765316 2316 24-25 -0.8583719040763285 2316 26-27 -0.7702378075017506 2316 28-29 -0.894167140193538 2316 30-31 -0.8519895996781983 2316 32-33 -0.6223734771908198 2316 34-35 -0.7588366216000608 2316 36-37 -0.6171686188514585 2316 38-39 -0.9317759127079484 2316 40-41 -0.9690669000313434 2316 42-43 -0.8920485058933778 2316 44-45 -0.9057248536492963 2316 46-47 -0.9997494748272473 2316 48-49 -1.020246748282787 2316 50-51 -1.0248746517342866 2316 52-53 -0.9681742339772299 2316 54-55 -0.8487371752164563 2316 56-57 -0.06455078139709514 2316 58-59 2.8144493408972373 2316 60-61 2.9779276733868407 2316 62-63 0.3437672920833563 2316 64-65 -0.15823252532272036 2316 66-67 -2.285554186393025 2316 68-69 -3.1613949621565673 2316 70-71 -3.2884466266649195 2316 72-73 -0.7353233393874739 2316 74-75 5.248309914897931 2316 76-77 5.311159455625848 2316 78-79 4.485028289390879 2316 80-81 2.7620684725130538 2316 82-83 1.0004858927697988 2316 84-85 0.6047623063846466 2316 86-87 0.6109970912188949 2316 88-89 0.6014201464958688 2316 90-91 2.055603159756899 2316 92-93 3.7001188366224405 2316 94-95 6.137533174427158 2316 96-97 3.4592588079155924 2316 98-99 5.541003108928685 2316 100-101 5.702601767850291 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7.0 3 17.0 4 46.0 5 125.0 6 268.0 7 673.0 8 1464.0 9 2497.0 10 4090.0 11 6617.0 12 9671.0 13 13138.0 14 16274.0 15 21590.0 16 28653.0 17 37536.0 18 39421.0 19 36363.0 20 38466.0 21 39172.0 22 39872.0 23 40377.0 24 42750.0 25 45305.0 26 55805.0 27 79455.0 28 112382.0 29 149974.0 30 183536.0 31 202465.0 32 214247.0 33 215699.0 34 222273.0 35 237632.0 36 239915.0 37 186881.0 38 76811.0 39 5557.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.316304864127076 19.425257516722198 14.040561032946345 22.217876586204383 2 14.856955883364204 22.183569819539 39.6717228392249 23.287751457871902 3 20.11506519970941 26.977269919656855 30.482493338738152 22.42517154189559 4 12.186884450700521 16.871198091745228 38.828330360185355 32.11358709736889 5 12.42153985268923 39.840856888388885 34.10840844791426 13.62919481100763 6 29.431180502193783 37.506356189927864 18.332460655845466 14.730002652032887 7 26.859993063913993 34.61231585938332 21.183244894458912 17.344446182243768 8 25.35264103941556 34.1458300749596 20.513361032343468 19.98816785328138 9 26.619043409471608 17.34463507347491 20.925559476937515 35.11076204011596 10-11 24.26438198944778 26.63732808064598 27.99211265775251 21.10617727215373 12-13 26.90156802388794 24.295775712063275 27.882574632814332 20.92008163123445 14-15 23.3367730562968 25.433719598544098 27.743509624329743 23.485997720829364 16-17 21.122100802938846 28.911162942864937 28.280549567703527 21.68618668649269 18-19 20.752323752215705 28.51152544831682 30.24508389081727 20.491066908650208 20-21 22.490901109395978 26.931507284023652 29.084829540775193 21.49276206580517 22-23 21.583316522013764 26.992840266774863 28.86764240321024 22.55620080800113 24-25 22.07180175325162 26.904511084049275 29.514819375491236 21.508867787207862 26-27 22.376023933301674 26.32972966234143 30.10491408650397 21.189332317852923 28-29 21.23769336302485 27.27479994778988 30.227391687144276 21.26011500204099 30-31 21.38022371100953 27.521773608871065 29.72170809819951 21.376294581919886 32-33 21.28152768542586 27.45566478268754 29.403864172157405 21.858943359729192 34-35 22.17645080979384 26.66093684476968 29.28899836416733 21.87361398126915 36-37 22.4083466947829 26.42018455153644 29.89140123842252 21.280067515258143 38-39 22.391727966752768 26.486325442963697 30.14497268718203 20.976973903101502 40-41 21.78884634784325 26.793863804964218 30.254945049959055 21.16234479723348 42-43 21.112782398395563 27.61654771033642 29.296796422083982 21.97387346918403 44-45 21.56273190228648 27.569120470549528 28.65411830100763 22.214029326156357 46-47 21.899830775684862 27.385867170161465 28.000924107309128 22.713377946844545 48-49 22.217282065852412 27.15329223299668 28.37647242967413 22.252953271476777 50-51 22.17190336283848 27.634089269285855 28.687893210602194 21.50611415727347 52-53 21.98115989143501 27.8828314596548 28.537283304730437 21.598725344179755 54-55 21.088681954543066 27.163641710193055 29.11559190282544 22.63208443243844 56-57 21.861218647911315 28.07955184042453 27.90909333198751 22.15013617967665 58-59 21.052585528375552 27.101551381696353 29.580505719771338 22.265357370156757 60-61 21.2066303092052 27.02566248510375 29.720348504007205 22.047358701683844 62-63 22.640134404204705 26.87524958055355 28.49294714162499 21.991668873616753 64-65 22.107988623948042 27.37719515641316 28.65656654189479 21.858249677744013 66-67 21.230742499157476 27.32833770427009 28.99864337070349 22.442276425868947 68-69 20.640574349645092 27.557428389994055 29.778260940186605 22.023736320174248 70-71 21.428303510145685 27.569521378923657 28.48043850236362 22.521736608567036 72-73 20.872053939387413 27.00124834054545 28.92411966224812 23.20257805781901 74-75 20.93598448015858 27.67391957681229 28.797524110102724 22.592571832926406 76-77 20.352265607057564 27.09593317353953 29.480586423082915 23.071214796319985 78-79 21.2977616186023 27.09660077940161 28.766017102005108 22.839620499990975 80-81 21.323976945887534 28.035923932237495 28.113530600779924 22.52656852109505 82-83 21.49202679915762 27.692557319570454 27.824139075455882 22.991276805816042 84-85 21.44526688525383 27.216916213417537 27.711117382363952 23.626699518964685 86-87 21.700298848486028 27.298272549592582 28.01586025806259 22.985568343858798 88-89 22.072718982117852 26.673109118140342 27.937339477840318 23.31683242190149 90-91 21.27226467926638 26.90669610495445 28.50904232385591 23.311996891923265 92-93 21.004453245645553 27.663570265858556 28.32994122123973 23.00203526725616 94-95 19.93086815187653 27.775388223181857 28.45900420396374 23.834739420977876 96-97 19.305505712279455 27.611434044336868 28.241677068950388 24.84138317443329 98-99 20.407094112950748 27.18986066493052 27.84760583192351 24.55543939019522 100-101 19.95412142645629 27.188040800286128 28.69034849975317 24.167489273504412 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1052.0 1 786.0 2 544.0 3 626.0 4 647.5 5 709.0 6 933.0 7 1358.5 8 1937.0 9 2364.0 10 2484.0 11 2561.0 12 2794.5 13 2973.0 14 3246.5 15 3573.5 16 3841.5 17 4154.5 18 4544.5 19 4913.0 20 5362.0 21 5990.0 22 6929.5 23 8171.5 24 9674.5 25 12028.0 26 15324.5 27 19316.5 28 23347.5 29 27746.0 30 32946.5 31 38591.5 32 44814.5 33 51270.0 34 57736.5 35 63516.5 36 68826.5 37 73627.5 38 76784.0 39 78827.5 40 80533.5 41 82944.0 42 85967.5 43 89227.5 44 93674.5 45 99602.0 46 109071.0 47 124384.5 48 144384.5 49 139035.5 50 113154.0 51 98178.0 52 87959.5 53 79499.0 54 72507.0 55 65267.0 56 57736.0 57 50455.0 58 43907.0 59 37440.5 60 30767.5 61 24620.5 62 19339.0 63 14714.5 64 10738.5 65 7773.0 66 5541.5 67 3912.0 68 2620.0 69 1685.5 70 1192.5 71 864.0 72 639.0 73 476.0 74 352.5 75 256.5 76 148.5 77 50.0 78 19.5 79 12.5 80 6.0 81 4.0 82 2.0 83 1.5 84 1.5 85 2.0 86 4.0 87 3.5 88 1.5 89 0.5 90 0.0 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.026180324636025487 2 0.01360016864209116 3 1.8889123114015503E-4 4 0.0 5 0.06550747895940576 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 3.9667158539432554E-4 16-17 0.0 18-19 5.288954471924341E-4 20-21 0.0 22-23 0.0 24-25 5.6667369342046515E-5 26-27 1.1333473868409303E-4 28-29 0.0011144582637269147 30-31 0.004873393763415999 32-33 0.005515623949292526 34-35 0.0021155817887697362 36-37 0.08653107298530502 38-39 0.07986321252605755 40-41 0.08537883647535006 42-43 0.08908110460569711 44-45 0.09287781835161422 46-47 0.08732441615609367 48-49 0.07689762019715711 50-51 0.07157088747900474 52-53 0.06067186344221779 54-55 0.04960283729740471 56-57 0.06828418005716604 58-59 0.6927397010834045 60-61 0.33161744538965615 62-63 0.0032111509293826354 64-65 0.0023422512661379225 66-67 1.0730344167378787 68-69 2.3152020418386523 70-71 2.302621885844718 72-73 1.7670396890699225 74-75 1.2595078401194397 76-77 0.7277790244599033 78-79 0.5620836365037593 80-81 0.037419352888864714 82-83 9.444561557007752E-5 84-85 0.001020012648156837 86-87 0.0012277930024110076 88-89 1.8889123114015505E-5 90-91 0.330125204663649 92-93 0.05392844649051426 94-95 0.9256048108329877 96-97 1.6256168243152882 98-99 0.6299900340986451 100-101 0.553167970393944 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2647026.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 43.37271518767222 #Duplication Level Percentage of deduplicated Percentage of total 1 69.04142726665846 29.945141609871644 2 15.151332899601885 13.143088931360811 3 6.209287647221086 8.079409939237545 4 3.1395636276561834 5.446855961436266 5 1.7488366965410747 3.792589797441279 6 1.1193637507558192 2.9129907091761993 7 0.7577955804183385 2.300735631597295 8 0.5351523656075625 1.8568808908404677 9 0.37391033566732845 1.459575584516336 >10 1.7322011056967384 13.296679280592958 >50 0.11707227467416197 3.4814469261057712 >100 0.06506014858715395 5.265895558584241 >500 0.0043671363660416655 1.2333918605518595 >1k 0.0034937090928300663 3.2173199807380835 >5k 7.86084545886765E-4 2.3838580259502797 >10k+ 3.4937090928300663E-4 2.1841393119990364 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 17553 0.6631215560406283 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 15135 0.5717737566612493 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 12535 0.47355031646836865 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12432 0.46965915710688144 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 9394 0.3548888450661233 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9109 0.34412204489113446 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 7056 0.2665633053849868 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 6824 0.2577987522600836 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 6730 0.25424759711464867 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6683 0.2524720195419312 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 6301 0.23804072948282337 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 5459 0.20623144615882125 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 5371 0.20290696049075452 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 4962 0.18745565778348985 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 4563 0.17238213753850548 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 3671 0.13868394190310182 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 3572 0.13494389552652675 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 3288 0.12421487359776595 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 3129 0.11820813244750902 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 3072 0.11605477241251126 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 3009 0.11367474290014529 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 2941 0.11110582215663918 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 2905 0.10974580529243007 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 2842 0.10736577578006412 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 3.7778246228031E-5 0.0 12-13 0.0 0.0 0.0 7.5556492456062E-5 0.0 14-15 0.0 0.0 0.0 9.444561557007752E-5 0.0 16-17 0.0 0.0 0.0 1.700021080261395E-4 7.5556492456062E-5 18-19 0.0 0.0 0.0 2.6444772359621703E-4 7.5556492456062E-5 20-21 0.0 0.0 0.0 2.6444772359621703E-4 7.5556492456062E-5 22-23 0.0 0.0 0.0 6.233410627625116E-4 9.444561557007752E-5 24-25 0.0 0.0 0.0 0.0011522365099549457 1.1333473868409302E-4 26-27 0.0 0.0 0.0 0.0019833579269716278 1.1333473868409302E-4 28-29 0.0 0.0 0.0 0.005647847811090635 1.1333473868409302E-4 30-31 0.0 0.0 0.0 0.015885752538887035 1.3222386179810851E-4 32-33 0.0 0.0 0.0 0.03549266233123513 2.2666947736818603E-4 34-35 0.0 0.0 0.0 0.06832195830339408 2.2666947736818603E-4 36-37 0.0 0.0 0.0 0.11624366364365141 2.2666947736818603E-4 38-39 0.0 0.0 0.0 0.1808255755704704 2.2666947736818603E-4 40-41 0.0 0.0 0.0 0.2549087164236392 2.6444772359621703E-4 42-43 0.0 0.0 0.0 0.3280851793673353 2.6444772359621703E-4 44-45 0.0 0.0 0.0 0.4165996102796119 2.6444772359621703E-4 46-47 0.0 0.0 0.0 0.5218309151477923 2.6444772359621703E-4 48-49 0.0 0.0 0.0 0.6297822537443909 2.6444772359621703E-4 50-51 0.0 0.0 0.0 0.740774741162346 2.6444772359621703E-4 52-53 0.0 0.0 0.0 0.8455338179526759 3.022259698242481E-4 54-55 0.0 0.0 0.0 0.9620608184430376 3.400042160522791E-4 56-57 0.0 0.0 0.0 1.0741677641247196 3.5889333916629454E-4 58-59 0.0 0.0 0.0 1.1664600196597994 3.7778246228031007E-4 60-61 0.0 0.0 0.0 1.2521033038587457 3.7778246228031007E-4 62-63 5.666736934204651E-5 0.0 0.0 1.3225786221971374 3.7778246228031007E-4 64-65 7.5556492456062E-5 3.7778246228031E-5 0.0 1.3953773026785532 3.7778246228031007E-4 66-67 7.5556492456062E-5 3.7778246228031E-5 0.0 1.4574280721080943 3.7778246228031007E-4 68-69 7.5556492456062E-5 3.7778246228031E-5 0.0 1.5330034536872703 3.7778246228031007E-4 70-71 7.5556492456062E-5 3.7778246228031E-5 0.0 1.6531760549386367 3.966715853943256E-4 72-73 7.5556492456062E-5 3.7778246228031E-5 0.0 1.8133369298223743 4.911172009644031E-4 74-75 7.5556492456062E-5 3.7778246228031E-5 0.0 2.0290884940306593 4.911172009644031E-4 76-77 7.5556492456062E-5 3.7778246228031E-5 0.0 2.282353856743379 4.911172009644031E-4 78-79 7.5556492456062E-5 3.7778246228031E-5 0.0 2.6052823055005883 4.911172009644031E-4 80-81 7.5556492456062E-5 3.7778246228031E-5 0.0 2.9971560536239537 4.911172009644031E-4 82-83 7.5556492456062E-5 3.7778246228031E-5 0.0 3.3827208346272384 4.911172009644031E-4 84-85 7.5556492456062E-5 5.666736934204651E-5 0.0 3.7570465873776833 4.911172009644031E-4 86-87 1.1333473868409302E-4 7.5556492456062E-5 0.0 4.109423179069642 4.911172009644031E-4 88-89 1.1333473868409302E-4 7.5556492456062E-5 0.0 4.419469245863093 4.911172009644031E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 16690 0.0 26.461054 1 TACATGG 16670 0.0 25.922304 2 ACATGGG 16585 0.0 24.686558 3 CATGGGA 12320 0.0 22.129591 4 CATGGGG 4315 0.0 21.753471 4 ATGGGAG 4335 0.0 21.326681 5 AGTACTT 10365 0.0 20.784155 12-13 GAGTACT 10145 0.0 19.957062 12-13 ATGGGAT 4595 0.0 19.09342 5 TACCTGG 1565 0.0 18.990656 2 GTACTTT 11070 0.0 18.35334 14-15 TTACGCG 140 5.7495527E-7 17.319918 62-63 GTCGGGA 465 0.0 17.246847 2 GCGTTTA 1235 0.0 17.081505 62-63 ATGGGGA 2325 0.0 16.635933 5 TACTTTT 12970 0.0 16.57335 14-15 AGTACAT 12385 0.0 16.455137 2 ACTTTTT 13245 0.0 15.784367 16-17 GCTTATA 2810 0.0 15.783712 1 CTTTGCG 600 0.0 15.725066 2 >>END_MODULE