FastQCFastQC Report
Fri 27 May 2016
SRR939645_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939645_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2790420
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87900.31500634313114156No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59630.21369542936188818No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA51350.18402247690311851No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43480.15581883730764542No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA42020.15058665003834548No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG98000.027.0690861
TACATGG100500.026.0656592
ACATGGG106900.023.8836253
GAGTACT75750.022.14437712-13
GTATCAA197400.021.468031
GTACTTT81700.020.35887714-15
CATGGGA67300.020.0258584
AGTACTT80850.019.74975612-13
TATCAAC219800.019.1294162
CAACGCA219850.019.038385
ATCAACG219850.019.0171363
TCAACGC220100.018.9736334
AACGCAG222900.018.8390396
CATGGGG47750.018.5847234
GTATAGG8100.017.5761641
ACGCAGA234700.017.5080647
AGTACAT73950.017.4552632
CGCAGAG234450.017.3859988
ACTTTTT101150.017.14922116-17
ATAGCGT1700.001801364116.7466476
Produced by FastQC (version 0.11.5)