##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939645_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2790420 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.007533274560817 31.0 30.0 33.0 26.0 34.0 2 30.150843242235936 31.0 30.0 34.0 25.0 34.0 3 30.185060313501193 31.0 30.0 34.0 26.0 34.0 4 33.76300341884018 35.0 35.0 37.0 28.0 37.0 5 33.75142738369134 35.0 33.0 37.0 28.0 37.0 6 33.840472402003996 35.0 35.0 37.0 28.0 37.0 7 33.644017388063446 35.0 33.0 37.0 28.0 37.0 8 33.64383354477104 35.0 33.0 37.0 28.0 37.0 9 34.88366410791207 37.0 34.0 39.0 28.0 39.0 10-11 34.76029558274382 37.0 34.0 39.0 27.0 39.0 12-13 34.6028701772493 37.0 34.0 39.0 27.0 39.0 14-15 35.25713458905828 38.0 33.5 40.0 27.0 41.0 16-17 34.98293464783079 38.0 33.0 40.0 25.5 41.0 18-19 34.949780498992986 38.0 33.0 40.0 25.5 41.0 20-21 35.07600934626329 38.0 34.0 40.0 25.5 41.0 22-23 34.928780792855555 38.0 33.5 40.0 25.0 41.0 24-25 34.80093623898911 38.0 33.0 40.0 25.0 41.0 26-27 34.92038761190072 38.0 34.0 40.0 25.0 41.0 28-29 34.79394678937221 38.0 33.5 40.0 24.5 41.0 30-31 34.57165534220655 38.0 33.0 40.0 24.0 41.0 32-33 34.34380111237735 38.0 33.0 40.0 24.0 41.0 34-35 34.11154951584349 38.0 33.0 40.0 23.5 41.0 36-37 33.82164100744691 38.0 32.5 40.0 21.5 41.0 38-39 33.648220160405955 38.0 32.0 40.0 20.0 41.0 40-41 33.364392994602966 37.5 31.5 40.0 18.5 41.0 42-43 33.05321654087915 37.0 31.0 40.0 17.0 41.0 44-45 32.8024019681625 37.0 31.0 40.0 15.5 41.0 46-47 32.35365518452419 37.0 30.5 40.0 13.5 41.0 48-49 32.000244766020884 37.0 30.0 39.5 9.0 41.0 50-51 31.40156069695601 35.5 29.5 38.5 9.0 40.0 52-53 31.446485654489287 36.0 30.0 39.0 8.0 40.0 54-55 31.4791753571147 36.0 30.0 39.0 8.0 40.0 56-57 31.337503135728674 36.0 29.5 39.0 6.5 40.0 58-59 30.932557464467713 36.0 28.5 39.0 2.0 40.0 60-61 30.496791522423145 35.0 28.0 39.0 2.0 40.0 62-63 30.063583797421177 35.0 28.0 38.0 2.0 40.0 64-65 29.42596401258592 34.0 26.5 38.0 2.0 40.0 66-67 28.879172848531763 34.0 26.0 38.0 2.0 39.5 68-69 28.35735265658933 34.0 26.0 37.0 2.0 39.0 70-71 27.783401602626128 33.0 25.0 36.5 2.0 39.0 72-73 27.023323012306392 32.0 23.0 36.0 2.0 38.5 74-75 26.54098719905964 32.0 22.0 35.0 2.0 38.0 76-77 26.12719303904072 32.0 21.5 35.0 2.0 37.5 78-79 25.53241913403717 31.0 20.0 35.0 2.0 37.0 80-81 24.85375391518123 31.0 18.0 34.5 2.0 36.0 82-83 24.170605141878283 30.5 12.0 34.0 2.0 36.0 84-85 23.49529551107002 30.0 7.0 34.0 2.0 35.0 86-87 22.79072989012407 29.5 2.0 34.0 2.0 35.0 88-89 21.956361049591102 29.0 2.0 34.0 2.0 35.0 90-91 20.689202700668716 27.5 2.0 33.5 2.0 35.0 92-93 19.01033751191577 25.0 2.0 32.0 2.0 34.0 94-95 18.733881279520645 25.0 2.0 32.0 2.0 34.0 96-97 18.08880616538012 24.5 2.0 32.0 2.0 34.0 98-99 17.082343338995564 21.0 2.0 32.0 2.0 34.0 100-101 15.47354000473047 2.0 2.0 30.5 2.0 34.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.17049248838482 1101 2 1.1728011379985475 1101 3 1.163335004657025 1101 4 1.2855618829377917 1101 5 1.051815020655063 1101 6 1.0680646154658788 1101 7 0.9283377947634506 1101 8 1.1408566299854215 1101 9 1.2241466651492132 1101 10-11 1.1348227137996716 1101 12-13 1.1379867527073557 1101 14-15 1.1639919852021237 1101 16-17 1.0047992693160523 1101 18-19 1.1227763694869068 1101 20-21 1.23795870525106 1101 22-23 1.3244130967575032 1101 24-25 1.1574189582471135 1101 26-27 1.3619518438496172 1101 28-29 1.497392248671055 1101 30-31 1.5223858490695577 1101 32-33 1.3507437791420713 1101 34-35 1.3659051968281588 1101 36-37 1.1504561722017357 1101 38-39 1.201214985243162 1101 40-41 1.3509394745977588 1101 42-43 1.6394546159536603 1101 44-45 1.3786071862335305 1101 46-47 1.099442602987061 1101 48-49 1.1146751989815726 1101 50-51 1.4113298842048927 1101 52-53 1.2920696765902022 1101 54-55 1.262571002755351 1101 56-57 1.021410594511135 1101 58-59 1.2357866442584573 1101 60-61 1.0607457560434845 1101 62-63 1.1483696526779035 1101 64-65 1.0018976625193474 1101 66-67 0.6836962009148984 1101 68-69 0.5968690324566985 1101 70-71 0.5699862927885029 1101 72-73 0.9376863361531349 1101 74-75 0.6968500487489209 1101 76-77 0.29973478052740177 1101 78-79 0.6665573968002825 1101 80-81 0.8035525454132504 1101 82-83 0.492774977595829 1101 84-85 0.3243956097785414 1101 86-87 -0.3261604874486217 1101 88-89 -0.7819486610615201 1101 90-91 -3.8888671401877595 1101 92-93 -8.955660169501565 1101 94-95 -7.502536743095458 1101 96-97 -4.4165058823744925 1101 98-99 -3.0893753430763304 1101 100-101 -2.5767590786247077 1102 1 1.0164367313012974 1102 2 0.9478653957202461 1102 3 0.8852128623508513 1102 4 1.0851291150024807 1102 5 0.9489522787450184 1102 6 0.9546486596208013 1102 7 0.9733271909750059 1102 8 0.8969526376337811 1102 9 1.184905717330487 1102 10-11 0.9561856855427564 1102 12-13 0.9109738030110108 1102 14-15 1.1295296727633044 1102 16-17 1.1206119885424926 1102 18-19 1.1012670313723447 1102 20-21 1.1660914869351515 1102 22-23 1.2875178695299851 1102 24-25 1.1904938433586878 1102 26-27 1.0787612929658579 1102 28-29 1.2059189046209227 1102 30-31 1.2560566517319458 1102 32-33 1.3688287548044897 1102 34-35 1.292607562853476 1102 36-37 1.2272286494797342 1102 38-39 1.2335142072573788 1102 40-41 1.321787953747382 1102 42-43 1.163822537629862 1102 44-45 0.9739326406829463 1102 46-47 0.9498489223279947 1102 48-49 0.975591088330539 1102 50-51 1.0459876015563303 1102 52-53 1.2404344154440565 1102 54-55 1.215735356044135 1102 56-57 1.0619286423051584 1102 58-59 1.0939330173731925 1102 60-61 1.0506611992129145 1102 62-63 1.0643266216390188 1102 64-65 0.919953567205507 1102 66-67 1.0004711282468222 1102 68-69 0.8424029288029367 1102 70-71 0.9804196826029496 1102 72-73 0.9178452075399157 1102 74-75 0.6469367559326358 1102 76-77 0.43525572979967464 1102 78-79 0.4621479740577108 1102 80-81 0.29395856130713227 1102 82-83 0.13195770853682376 1102 84-85 -0.023452857008429362 1102 86-87 -0.4195761922942971 1102 88-89 -1.0460008816757629 1102 90-91 -5.117076832586111 1102 92-93 -10.332648824702929 1102 94-95 -8.799845542422931 1102 96-97 -5.495111966525229 1102 98-99 -4.287727063677277 1102 100-101 -3.5226579356033696 1103 1 0.7830503809133482 1103 2 0.8348897105841928 1103 3 0.8379712474190129 1103 4 0.7658281128808824 1103 5 0.829210301205201 1103 6 0.3740278085867743 1103 7 0.7860982163090355 1103 8 0.46489682413174904 1103 9 0.6511669064750834 1103 10-11 0.6781861454896045 1103 12-13 0.6423099706610884 1103 14-15 0.7231607049468991 1103 16-17 0.7370640225388456 1103 18-19 0.6301264900358632 1103 20-21 0.7147420337700368 1103 22-23 0.513906299605658 1103 24-25 0.5348363041184427 1103 26-27 0.644307992736934 1103 28-29 0.6996406853101078 1103 30-31 0.8289601571997451 1103 32-33 0.8828190804788463 1103 34-35 0.8730192594010688 1103 36-37 0.7568193905401372 1103 38-39 0.7261884231555555 1103 40-41 0.660159929417631 1103 42-43 0.6106276718278991 1103 44-45 0.5755516646139753 1103 46-47 0.6303033321648996 1103 48-49 0.4420229651839094 1103 50-51 0.37221193428871757 1103 52-53 0.5858607017923987 1103 54-55 0.7212303145524146 1103 56-57 0.6042436143471228 1103 58-59 0.38698395951971065 1103 60-61 0.27060058439523615 1103 62-63 0.3548492348650818 1103 64-65 0.13869731118387207 1103 66-67 0.1400255643900401 1103 68-69 0.2596563402132581 1103 70-71 0.39972437974118336 1103 72-73 0.3147123297782386 1103 74-75 0.2277761368684459 1103 76-77 -0.15294018108534857 1103 78-79 -0.12613107470161822 1103 80-81 -0.3531326057831734 1103 82-83 -0.36713632301861665 1103 84-85 -0.5765786480590975 1103 86-87 -1.0817642598171453 1103 88-89 -1.1547559754216472 1103 90-91 -2.782893047973598 1103 92-93 -9.037036326060985 1103 94-95 -5.8669253983503715 1103 96-97 -3.3552889523071343 1103 98-99 -2.302204359454535 1103 100-101 -1.7933596799269687 1104 1 0.9369705120267575 1104 2 0.8250708934394666 1104 3 0.9044703287490101 1104 4 0.9908667021748343 1104 5 1.0054578499153237 1104 6 0.8163082748021395 1104 7 0.9371976568609028 1104 8 0.7089522690231504 1104 9 0.8691993807510343 1104 10-11 0.8017645262080961 1104 12-13 0.8478664424528901 1104 14-15 1.0149494958844016 1104 16-17 1.0283204229501237 1104 18-19 0.9278595783905246 1104 20-21 1.0824840897870018 1104 22-23 0.9860535908820296 1104 24-25 0.8832131057037671 1104 26-27 0.8706373584809768 1104 28-29 0.9518194664811048 1104 30-31 0.985714765367014 1104 32-33 0.9983922764454647 1104 34-35 0.947814459124821 1104 36-37 1.005683357803015 1104 38-39 0.7693760921045012 1104 40-41 0.7925000769717556 1104 42-43 0.8355567422575447 1104 44-45 0.9054576587746865 1104 46-47 0.5825796495479878 1104 48-49 0.7148741406434382 1104 50-51 0.7666747893553172 1104 52-53 0.9594850804339075 1104 54-55 1.001135664484508 1104 56-57 0.8002497834803215 1104 58-59 0.7051189949909364 1104 60-61 0.8254438951118495 1104 62-63 0.6440469383189189 1104 64-65 0.6611491937487237 1104 66-67 0.570773307657209 1104 68-69 0.790276912752109 1104 70-71 0.8170064565280839 1104 72-73 0.6198628086977891 1104 74-75 0.30988217174440535 1104 76-77 0.33556484776320517 1104 78-79 0.3628280064737659 1104 80-81 0.2820135439200939 1104 82-83 0.35980055030800173 1104 84-85 0.24415507679344017 1104 86-87 0.021282941524912502 1104 88-89 -0.10554193433016579 1104 90-91 -0.6704710318472671 1104 92-93 -4.233149565989759 1104 94-95 -1.9341205042744392 1104 96-97 -1.0166025086810038 1104 98-99 -0.9292109383171336 1104 100-101 -0.8107697005165164 1105 1 0.9285469772708694 1105 2 0.9875134582179754 1105 3 0.9737896807023496 1105 4 0.989569281831308 1105 5 0.7947288676017408 1105 6 0.7661771674651021 1105 7 1.0664698739721032 1105 8 0.7863418358138787 1105 9 1.0951217575516026 1105 10-11 1.0225702717080267 1105 12-13 1.0694349072784846 1105 14-15 1.2355943398252052 1105 16-17 1.3945437277737227 1105 18-19 1.2850133178525027 1105 20-21 1.0409946466391062 1105 22-23 0.9998130985564373 1105 24-25 1.0979850427365179 1105 26-27 1.0083244336581103 1105 28-29 1.210040949199815 1105 30-31 1.157098021141799 1105 32-33 1.2727067222410327 1105 34-35 1.1803106407432509 1105 36-37 1.1879275326178984 1105 38-39 1.2133638477638087 1105 40-41 1.1895697163404506 1105 42-43 1.3291301859719837 1105 44-45 1.2695464340726588 1105 46-47 1.3453909098499608 1105 48-49 1.1990910502361558 1105 50-51 1.0518790552244077 1105 52-53 1.1556092795945645 1105 54-55 1.386999996781313 1105 56-57 1.2997324996415536 1105 58-59 1.193357996967439 1105 60-61 1.007770881276354 1105 62-63 1.1373050864325691 1105 64-65 1.1031291888744654 1105 66-67 1.117293635993363 1105 68-69 0.9564538993632148 1105 70-71 1.149725807715832 1105 72-73 1.051249968983047 1105 74-75 0.8781203826109412 1105 76-77 0.5939850649611742 1105 78-79 0.45508699546074993 1105 80-81 0.44971667097363266 1105 82-83 0.5610577771613343 1105 84-85 0.4724276243844443 1105 86-87 0.08989367586636021 1105 88-89 0.036040974632214784 1105 90-91 -0.9313075157973039 1105 92-93 -6.954137075384189 1105 94-95 -2.7104482611331715 1105 96-97 -0.9639439326023833 1105 98-99 -0.4933346117724646 1105 100-101 -0.20266407712824375 1106 1 0.7351007827841016 1106 2 0.7859363310940779 1106 3 0.6802100115287999 1106 4 0.8593847546206206 1106 5 0.6909771633655595 1106 6 0.7275252158165983 1106 7 0.7017155736867693 1106 8 0.6460127594195626 1106 9 0.894150055525337 1106 10-11 0.8034310211764719 1106 12-13 0.8850213451782025 1106 14-15 0.8167460034106568 1106 16-17 0.9645458076086442 1106 18-19 0.7329817080405618 1106 20-21 0.9701834282486885 1106 22-23 0.9410414046498019 1106 24-25 0.7250427227470126 1106 26-27 0.8540038361056403 1106 28-29 0.9671758143409832 1106 30-31 0.8859227593788788 1106 32-33 0.8956702281673827 1106 34-35 0.7570674541733879 1106 36-37 0.979694408218414 1106 38-39 0.8722714469918387 1106 40-41 0.9655113868906469 1106 42-43 0.9361462324961778 1106 44-45 0.6389014658344863 1106 46-47 0.8260741967143801 1106 48-49 0.7880556274811354 1106 50-51 0.611188050766625 1106 52-53 0.7019465037388812 1106 54-55 0.7877525212581347 1106 56-57 0.7248452383873563 1106 58-59 0.7818274473720486 1106 60-61 0.6860296166815445 1106 62-63 0.6889780134067145 1106 64-65 0.5539366916785653 1106 66-67 0.5970058514826526 1106 68-69 0.6661930318316145 1106 70-71 0.5645469110373789 1106 72-73 0.7107777085020501 1106 74-75 0.5710475380681537 1106 76-77 0.4729944460283164 1106 78-79 0.4872523716356163 1106 80-81 0.4426976551336459 1106 82-83 0.41263376779931704 1106 84-85 0.11441669283518507 1106 86-87 0.21845585301074877 1106 88-89 0.15062818521096588 1106 90-91 -0.2982137817237067 1106 92-93 -3.8472951148527024 1106 94-95 -1.5168200318215845 1106 96-97 -0.6076432481225353 1106 98-99 -0.3299440683075261 1106 100-101 -0.10437919860545009 1107 1 0.6399053794813803 1107 2 0.6657576831554444 1107 3 0.6134728425935947 1107 4 0.6377385329789576 1107 5 0.41102518591615933 1107 6 0.4831777700049358 1107 7 0.6595610395491818 1107 8 0.607836097442501 1107 9 0.5534285401189436 1107 10-11 0.49558286559555853 1107 12-13 0.505451307756303 1107 14-15 0.5973959153461053 1107 16-17 0.6920061247675875 1107 18-19 0.45572944851880237 1107 20-21 0.49307204668901505 1107 22-23 0.5848627415221657 1107 24-25 0.5335723580654843 1107 26-27 0.4560097332343602 1107 28-29 0.530752342528281 1107 30-31 0.5117782322598643 1107 32-33 0.3285780796771931 1107 34-35 0.3249062052326579 1107 36-37 0.44213840669699067 1107 38-39 0.31063018708972123 1107 40-41 0.30030805366491364 1107 42-43 0.25654550063958936 1107 44-45 0.23291541819545358 1107 46-47 0.34168234839678746 1107 48-49 0.29651108985640207 1107 50-51 0.398133802625221 1107 52-53 0.557623360324996 1107 54-55 0.4710110171295767 1107 56-57 0.5267096879665267 1107 58-59 0.467486311323551 1107 60-61 0.5173700901099743 1107 62-63 0.537307191916053 1107 64-65 0.4662156047285073 1107 66-67 0.31846075502934923 1107 68-69 0.5667758463657186 1107 70-71 0.592685163834215 1107 72-73 0.5722964438153966 1107 74-75 0.7093247005860093 1107 76-77 0.34216261419078364 1107 78-79 0.3826425105579965 1107 80-81 0.22259185110083735 1107 82-83 0.2569541898509584 1107 84-85 0.1031084200838066 1107 86-87 0.12484073843823396 1107 88-89 0.24616704974472015 1107 90-91 -0.055277330595259855 1107 92-93 -3.5238987408463966 1107 94-95 -1.3059497718175734 1107 96-97 -0.6732273189133977 1107 98-99 -0.41305042104290735 1107 100-101 -0.40563045565330214 1108 1 0.3720819881346742 1108 2 0.43557124322807184 1108 3 0.04164342848438807 1108 4 -0.0989480619459826 1108 5 -0.7545883830134699 1108 6 -0.10118784710680018 1108 7 0.013207025112265569 1108 8 0.2614546262941886 1108 9 0.35085646625153544 1108 10-11 0.4665487218463866 1108 12-13 0.21367414996878153 1108 14-15 0.27138378125107465 1108 16-17 0.5625388735386849 1108 18-19 0.2927809519951765 1108 20-21 0.22950267420772263 1108 22-23 0.3987576088885376 1108 24-25 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0.4199275129846498 2311 72-73 0.40056789452433605 2311 74-75 0.6243623831107392 2311 76-77 0.646086511874536 2311 78-79 0.6003329882113029 2311 80-81 0.7389253824039592 2311 82-83 0.8494917529111632 2311 84-85 0.8096801636514996 2311 86-87 0.7747366336371257 2311 88-89 1.0215646959522324 2311 90-91 1.978815114468123 2311 92-93 2.8536269098939435 2311 94-95 2.192608803346097 2311 96-97 1.986802069359964 2311 98-99 1.7373388626477997 2311 100-101 1.5913730327108553 2312 1 0.16340404669077557 2312 2 -0.018991404526062183 2312 3 -0.12145531674201848 2312 4 0.04254675529351459 2312 5 0.013347605348052127 2312 6 0.0740876575304128 2312 7 0.24282695600879123 2312 8 -0.435879434444125 2312 9 0.09545002871743691 2312 10-11 -0.019125037018859814 2312 12-13 0.06877144038521976 2312 14-15 -0.4053174371522701 2312 16-17 -0.01913052089666678 2312 18-19 0.09844743681060208 2312 20-21 0.21276144853032974 2312 22-23 0.06923587766468131 2312 24-25 0.201056789997061 2312 26-27 0.11402810115240669 2312 28-29 0.1045974222271866 2312 30-31 0.34538999586832375 2312 32-33 0.26525483767465374 2312 34-35 0.22030602828103696 2312 36-37 0.2845960556147773 2312 38-39 0.5311475303278215 2312 40-41 0.4231653418953627 2312 42-43 0.2879657211789848 2312 44-45 0.26873907713673617 2312 46-47 0.4117317309034476 2312 48-49 0.33431400644887077 2312 50-51 0.2996028403306177 2312 52-53 0.13415352548926762 2312 54-55 0.19211801067621082 2312 56-57 0.23641941380457254 2312 58-59 0.274900754081397 2312 60-61 0.3474462132298619 2312 62-63 0.3303650912797629 2312 64-65 0.45477961118470134 2312 66-67 0.43355761201794607 2312 68-69 0.11484139196185339 2312 70-71 0.10610635110613131 2312 72-73 0.160576649260193 2312 74-75 0.4984721386034714 2312 76-77 0.567950063062785 2312 78-79 0.6001590255720295 2312 80-81 0.6264389942931494 2312 82-83 0.7898906540408674 2312 84-85 0.8867819471593847 2312 86-87 0.8841921796329117 2312 88-89 1.0601301160393923 2312 90-91 1.5406640888055385 2312 92-93 2.4894031595462316 2312 94-95 1.879543869513352 2312 96-97 1.3674927554854328 2312 98-99 1.6395760103289767 2312 100-101 1.584186063035446 2313 1 -0.05363106537654616 2313 2 -0.7688356750320544 2313 3 -0.1423415162345023 2313 4 0.22619526567441284 2313 5 -0.5543624417783519 2313 6 -0.20743276426914292 2313 7 -0.9938347913790935 2313 8 -0.81606114030415 2313 9 -0.8629449922288259 2313 10-11 -1.7986799638410176 2313 12-13 -0.33011952322856075 2313 14-15 0.011343902679080031 2313 16-17 -1.4706423947343268 2313 18-19 -0.9258839559816607 2313 20-21 -1.2495573228797952 2313 22-23 -0.8737232079500714 2313 24-25 -0.12132321141866242 2313 26-27 -0.7281540530198001 2313 28-29 -2.1217231145462065 2313 30-31 -0.3823744171537484 2313 32-33 -0.13583264111633042 2313 34-35 0.05110335900028673 2313 36-37 0.3033957265665137 2313 38-39 -0.1939143002873962 2313 40-41 -0.06636512080999779 2313 42-43 -0.023582822020934202 2313 44-45 -0.5714325899625052 2313 46-47 -0.7919336501726271 2313 48-49 -0.19908380304205053 2313 50-51 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-1.6620493935896548 2314 9 -2.0493433866486157 2314 10-11 -1.1583091239016667 2314 12-13 -2.110164141643992 2314 14-15 -3.0621138981219787 2314 16-17 -2.3508497490487557 2314 18-19 -2.8498663380705125 2314 20-21 -2.3669101184068495 2314 22-23 -3.3433418610447596 2314 24-25 -2.0194490694443417 2314 26-27 -2.1544605854876764 2314 28-29 -1.4551416832913233 2314 30-31 -1.666151244702526 2314 32-33 -2.7422314438882793 2314 34-35 -0.8896449582215027 2314 36-37 -0.5877212203075217 2314 38-39 -0.5468337373750174 2314 40-41 -0.9085231280341546 2314 42-43 -0.7793070983193573 2314 44-45 -1.3591306245570678 2314 46-47 -1.861694837617506 2314 48-49 -2.1573293082514553 2314 50-51 -1.138298089053361 2314 52-53 -0.7538249739735434 2314 54-55 -0.5172833749547294 2314 56-57 -0.6437907997600227 2314 58-59 -0.7002439579595823 2314 60-61 -0.6966263804923294 2314 62-63 -0.6969940778812891 2314 64-65 -0.8415454827223137 2314 66-67 -0.751839517106685 2314 68-69 -0.6593844046896002 2314 70-71 -0.9348077508913697 2314 72-73 -0.7043478608881308 2314 74-75 -0.6674316701818306 2314 76-77 -0.5710839021091907 2314 78-79 -0.49333674355351675 2314 80-81 -0.38809081231317677 2314 82-83 -0.30266358970347795 2314 84-85 -0.4818262108925495 2314 86-87 -0.3325509239149156 2314 88-89 -0.16940398322764239 2314 90-91 0.6161926310870278 2314 92-93 1.3976186268310862 2314 94-95 0.944456723914179 2314 96-97 0.8192845838351275 2314 98-99 0.6337241775436269 2314 100-101 0.5885682463620086 2315 1 -7.353780354085284 2315 2 -3.1651685541342403 2315 3 -1.5959656732306762 2315 4 -1.054767386109674 2315 5 -1.5385052902416305 2315 6 -1.849202790221078 2315 7 -3.1566441097719817 2315 8 -3.0464765448157927 2315 9 -3.5420506487085603 2315 10-11 -2.378458070478281 2315 12-13 -2.928141664827166 2315 14-15 -4.7821646423635755 2315 16-17 -4.519847779543333 2315 18-19 -3.3141992936605646 2315 20-21 -4.37144287398948 2315 22-23 -5.635056564428943 2315 24-25 -3.727876074376841 2315 26-27 -4.3936316900920005 2315 28-29 -2.9403319086927056 2315 30-31 -2.8236822556789143 2315 32-33 -1.9483394762824915 2315 34-35 -2.6546088870136195 2315 36-37 -3.3568298990139347 2315 38-39 -2.4984446050273093 2315 40-41 -3.720503976668276 2315 42-43 -7.7163516501913705 2315 44-45 -4.458097289258596 2315 46-47 -3.0100713603268616 2315 48-49 -5.86105619331051 2315 50-51 -6.641221560011889 2315 52-53 -7.087668533668992 2315 54-55 -4.818154477157215 2315 56-57 -4.0916617906046575 2315 58-59 -4.487260397434408 2315 60-61 -3.399257973804744 2315 62-63 -3.507267548730642 2315 64-65 -2.3503812910383424 2315 66-67 -2.741296876877687 2315 68-69 -2.1950463179047226 2315 70-71 -1.9343321873879198 2315 72-73 -2.2812590126121037 2315 74-75 -3.0173243517879165 2315 76-77 -3.009249674637868 2315 78-79 -2.206577027581524 2315 80-81 -2.133132222147168 2315 82-83 -2.338068342718323 2315 84-85 -2.112640072979133 2315 86-87 -2.3565493037674408 2315 88-89 -1.9273125737211778 2315 90-91 -0.9972162181497346 2315 92-93 -0.1376722027411894 2315 94-95 -0.18634529275455947 2315 96-97 -0.17112161028124717 2315 98-99 -0.24629396723467423 2315 100-101 -0.08049348737046813 2316 1 -4.2036653751610125 2316 2 -2.7040394027101406 2316 3 -4.450034063233797 2316 4 -4.351089857078623 2316 5 -3.9744463096465736 2316 6 -4.44645429988088 2316 7 -3.039406029291225 2316 8 -1.6962325514540275 2316 9 -2.032987861682315 2316 10-11 -3.1053546613082688 2316 12-13 -4.146331240819926 2316 14-15 -3.2235107940504193 2316 16-17 -3.039986102333195 2316 18-19 -3.417150793663936 2316 20-21 -5.706043838734516 2316 22-23 -6.532785113111963 2316 24-25 -4.396467683610183 2316 26-27 -3.9803579122483512 2316 28-29 -4.576696744454743 2316 30-31 -6.065373063428314 2316 32-33 -4.407189204218106 2316 34-35 -5.999044479727729 2316 36-37 -9.516079975928548 2316 38-39 -6.383535746035935 2316 40-41 -5.511499408950854 2316 42-43 -4.501363125626973 2316 44-45 -3.3645395898251778 2316 46-47 -5.6014020729491705 2316 48-49 -2.997560317505716 2316 50-51 -4.370335705788097 2316 52-53 -5.3791069267486975 2316 54-55 -6.934151705985485 2316 56-57 -4.9764697510986196 2316 58-59 -6.035190103092855 2316 60-61 -6.361364951363644 2316 62-63 -6.502982394261398 2316 64-65 -5.393935285096266 2316 66-67 -6.525870949579396 2316 68-69 -5.321270158515187 2316 70-71 -6.365406364513852 2316 72-73 -8.095897042340443 2316 74-75 -7.906098636032883 2316 76-77 -6.961969813241367 2316 78-79 -6.032392883893802 2316 80-81 -6.119488805027434 2316 82-83 -4.963030278406478 2316 84-85 -6.588260218095115 2316 86-87 -5.136823793492322 2316 88-89 -5.042196617925185 2316 90-91 -5.417822990228718 2316 92-93 -4.044515020712936 2316 94-95 -4.012108880815617 2316 96-97 -4.772043462789341 2316 98-99 -5.65368753874505 2316 100-101 -4.717782814317081 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 17134.0 3 6942.0 4 7156.0 5 8052.0 6 9043.0 7 10361.0 8 12701.0 9 15554.0 10 18501.0 11 21707.0 12 25448.0 13 28101.0 14 30345.0 15 33479.0 16 37368.0 17 40791.0 18 39249.0 19 37859.0 20 40885.0 21 44410.0 22 49040.0 23 54395.0 24 60316.0 25 67783.0 26 76618.0 27 85087.0 28 96083.0 29 108656.0 30 122984.0 31 140295.0 32 161102.0 33 184433.0 34 216131.0 35 256984.0 36 292155.0 37 253299.0 38 78652.0 39 1321.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.172260398530526 19.08066954047107 13.432847598694664 23.314222462303736 2 15.09963456504559 20.72059597429651 40.76434629042447 23.415423170233428 3 19.003013908747654 25.887438651065985 29.85875306968866 25.250794370497697 4 12.260442524859737 16.781435192492573 38.5735963036834 32.384525978964284 5 13.26094006032135 38.16889404161657 34.750098287642736 13.82006761041935 6 30.767128505349145 37.1429913819044 18.322625719863296 13.767254392883158 7 27.6599179072114 33.13731405280325 21.946566870685025 17.256201169300326 8 25.60863979041141 35.223846441348414 20.595436483881635 18.57207728435855 9 26.84769065120286 16.532967588703713 20.577977419764412 36.04136434032901 10-11 24.40688416817796 26.558715795091942 28.431610270654943 20.60278976607516 12-13 26.011594281914224 24.14995950394666 28.283229181836244 21.555217032302863 14-15 23.028832202015174 25.319119247020268 26.718652419100557 24.933396131864 16-17 22.172253113394326 28.4575101059658 27.226321084096305 22.14391569654357 18-19 21.940516357589146 27.636025078216974 29.13265785624147 21.29080070795241 20-21 22.901059257466848 26.81229207186513 28.823613084474587 21.46303558619344 22-23 22.511452787713562 26.725353925538624 28.552794943031607 22.2103983437162 24-25 22.470954680347617 26.80114715308407 28.865993995084605 21.861904171483705 26-27 22.446247653774236 26.891892376874736 28.84691303083919 21.814946938511838 28-29 22.081740252968032 26.994549776512667 29.084630542031032 21.83907942848827 30-31 22.208819594792832 27.074733006681672 28.870342140845423 21.84610525768007 32-33 22.13723787987702 27.027491143784175 28.760944438124202 22.074326538214603 34-35 22.53992343041227 26.93085931661544 28.614496689622232 21.914720563350055 36-37 22.64199120543278 26.74569790772846 28.712704463998296 21.899606422840467 38-39 22.52740785609938 26.902096780386557 28.874446881814738 21.69604848169933 40-41 22.463979905357824 26.79127138980466 28.925501805378005 21.819246899459507 42-43 22.215480218548567 27.10989380267726 28.538931453020005 22.135694525754165 44-45 22.331938271895076 27.30003496474015 28.261897205816737 22.106129557548034 46-47 22.472744172025724 27.17196112769867 27.8477046968305 22.507590003445106 48-49 22.56273163356998 27.19788862708895 27.924938295356387 22.314441443984677 50-51 22.498444778602163 27.414523847386207 27.96577041163022 22.121260962381413 52-53 22.526289903797394 27.49823866395144 27.81720876925466 22.158262662996506 54-55 22.232898057223395 27.281080216604774 27.97236097517022 22.513660751001616 56-57 22.40497343605777 27.708861777063852 27.66269248271298 22.22347230416539 58-59 22.459730992714572 27.294954386462255 27.788400552260672 22.456914068562497 60-61 22.500583103979103 27.179140307376535 28.060022032359576 22.260254556284785 62-63 22.649721008063235 27.120427527491486 27.956317905665006 22.273533558780276 64-65 22.315334480314668 27.25051137895535 28.01181710078493 22.42233703994505 66-67 22.12173262798848 27.50308495678549 27.761740507680365 22.613441907545667 68-69 22.058036785909966 27.484552336193406 27.951381974322846 22.50602890357378 70-71 22.13708943099642 27.276951797922855 27.696041008232687 22.889917762848043 72-73 22.253244591450233 27.236872449844896 27.572732420250308 22.937150538454567 74-75 22.141525420838644 27.487342486722333 27.45853359699899 22.91259849544003 76-77 22.37736579943948 27.06845953174148 27.70979251635778 22.844382152461264 78-79 22.14598733532385 27.206939544641745 27.73455660171105 22.912516518323358 80-81 21.940282110446645 27.513792636722755 27.548527786144927 22.99739746668567 82-83 22.109938107510445 27.45453112656035 27.41807309521147 23.01745767071774 84-85 22.13269242296495 27.11022861505429 27.361714865954763 23.395364096026004 86-87 22.122639274546234 27.10823156286701 27.655484119212865 23.113645043373886 88-89 22.28061735530959 26.80882305425015 27.624944802059286 23.28561478838097 90-91 21.78947739087816 27.016700466684583 28.026227472443765 23.167594669993488 92-93 21.536305673737658 27.081224940220412 28.211623882724286 23.170845503317643 94-95 21.87447046524385 26.995448514143423 27.47506340056809 23.65501762004463 96-97 21.88128797921493 26.892838830969062 27.342539305929176 23.88333388388683 98-99 21.87928366446273 26.796055105275123 27.203516101820004 24.12114512844215 100-101 22.00568712889778 26.712158909191796 27.419772784206053 23.86238117770437 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2784.0 1 1699.0 2 646.0 3 679.0 4 590.0 5 589.0 6 846.5 7 1505.5 8 2378.0 9 2710.0 10 2397.5 11 2092.5 12 2105.5 13 2177.0 14 2327.0 15 2616.5 16 2875.5 17 3082.0 18 3293.0 19 3661.0 20 4253.5 21 5026.5 22 6003.0 23 7390.5 24 9003.0 25 11014.5 26 14283.5 27 18498.5 28 22677.0 29 27141.0 30 32183.0 31 37732.5 32 43918.0 33 50285.0 34 57168.5 35 63669.0 36 70276.5 37 76826.5 38 80512.0 39 82834.5 40 85398.5 41 87657.0 42 89454.0 43 92194.5 44 96123.5 45 99544.5 46 102066.5 47 107739.0 48 121429.0 49 121742.0 50 108525.5 51 100345.5 52 95010.5 53 91545.5 54 88108.5 55 83234.5 56 76608.0 57 69178.0 58 61694.5 59 54041.5 60 45768.0 61 37552.0 62 29794.5 63 23188.5 64 17668.5 65 13158.5 66 9495.5 67 6825.5 68 4812.0 69 3197.0 70 2194.0 71 1528.0 72 1032.0 73 698.0 74 481.5 75 325.5 76 220.5 77 135.5 78 83.0 79 63.0 80 44.0 81 30.5 82 22.0 83 19.5 84 17.5 85 11.0 86 9.0 87 9.0 88 7.0 89 6.0 90 5.0 91 3.0 92 1.5 93 1.5 94 4.0 95 3.5 96 0.5 97 1.0 98 2.0 99 1.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08880383598168018 2 0.09973409020864242 3 0.09597121580263904 4 0.09962657951132804 5 0.0961862371972678 6 0.07945040531532888 7 0.09314010077336028 8 0.08894718357809936 9 0.08306993212491309 10-11 0.09016563814766235 12-13 0.08937722636735689 14-15 0.09564868371069589 16-17 0.09269213953455036 18-19 0.09539782541696233 20-21 0.10566509701048588 22-23 0.11526938597057074 24-25 0.1386708810860014 26-27 0.14442270339232086 28-29 0.14408225285082532 30-31 0.14576658710875065 32-33 0.14187828355588047 34-35 0.13634148264418977 36-37 0.14073150278452706 38-39 0.13327742777072984 40-41 0.1292816135205453 42-43 0.12827818034561106 44-45 0.11921144487209812 46-47 0.13833043054450586 48-49 0.15644598304197935 50-51 0.16639072254355974 52-53 0.17533202887020594 54-55 0.1717841758588313 56-57 0.1410540348764702 58-59 0.13232774994445282 60-61 0.12937120576830727 62-63 0.12584127120648506 64-65 0.111219816371729 66-67 0.12575167895872305 68-69 0.12288472703033952 70-71 0.13125264297130898 72-73 0.15902623977752453 74-75 0.17278760903376553 76-77 0.17816314389948468 78-79 0.17477655693408162 80-81 0.18122719877294458 82-83 0.18009833645114354 84-85 0.15707312877631324 86-87 0.15811239885035228 88-89 0.17882612653292335 90-91 0.26461966298980083 92-93 0.5306369650446886 94-95 0.17760767196336036 96-97 0.19151238881602053 98-99 0.20996839185498956 100-101 0.21726120082281522 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2790420.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.85978869492877 #Duplication Level Percentage of deduplicated Percentage of total 1 75.05785271019695 40.426000868662946 2 12.839914548570427 13.8311016889389 3 4.831952850057294 7.807438784638341 4 2.4006665598712544 5.171975745665893 5 1.3608657205288757 3.664797007492862 6 0.811861134340165 2.623600148711188 7 0.5537893615448598 2.087888459601396 8 0.39965128681030626 1.7220107087407577 9 0.28551266885595145 1.3839886812876043 >10 1.3396633343829074 12.697102394250114 >50 0.07840469310607834 2.8774342887286632 >100 0.037297781094462315 3.4878254086981744 >500 0.0014187990380991685 0.5270899497320343 >1k 9.458660253673566E-4 0.9680479069633756 >5k 2.0268557686443356E-4 0.7236979578877425 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8790 0.31500634313114156 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5963 0.21369542936188818 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 5135 0.18402247690311851 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4348 0.15581883730764542 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 4202 0.15058665003834548 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 3.583689910479426E-5 0.0 3.583689910479426E-5 0.0 10-11 0.0 3.583689910479426E-5 0.0 3.583689910479426E-5 0.0 12-13 0.0 3.583689910479426E-5 0.0 3.583689910479426E-5 0.0 14-15 0.0 3.583689910479426E-5 0.0 5.375534865719139E-5 0.0 16-17 0.0 5.375534865719139E-5 0.0 7.167379820958852E-5 3.583689910479426E-5 18-19 0.0 7.167379820958852E-5 0.0 1.2542914686677992E-4 3.583689910479426E-5 20-21 0.0 7.167379820958852E-5 0.0 2.1502139462876557E-4 3.583689910479426E-5 22-23 0.0 7.167379820958852E-5 0.0 3.7628744060033974E-4 3.583689910479426E-5 24-25 0.0 7.167379820958852E-5 0.0 6.80901082991091E-4 3.583689910479426E-5 26-27 0.0 7.167379820958852E-5 0.0 0.0010930254226962249 3.583689910479426E-5 28-29 0.0 7.167379820958852E-5 0.0 0.0027773596806215553 3.583689910479426E-5 30-31 0.0 7.167379820958852E-5 0.0 0.008815877179779389 3.583689910479426E-5 32-33 0.0 7.167379820958852E-5 0.0 0.0212154442700382 3.583689910479426E-5 34-35 0.0 7.167379820958852E-5 0.0 0.03847091118899664 3.583689910479426E-5 36-37 0.0 7.167379820958852E-5 0.0 0.06645952938984095 3.583689910479426E-5 38-39 0.0 7.167379820958852E-5 0.0 0.10960715591201325 3.583689910479426E-5 40-41 0.0 7.167379820958852E-5 0.0 0.1591695873739437 3.583689910479426E-5 42-43 0.0 7.167379820958852E-5 0.0 0.2098608811576752 3.583689910479426E-5 44-45 0.0 7.167379820958852E-5 0.0 0.26768371786326073 3.583689910479426E-5 46-47 0.0 7.167379820958852E-5 0.0 0.3355050494190839 3.583689910479426E-5 48-49 0.0 7.167379820958852E-5 0.0 0.4139341031099261 3.583689910479426E-5 50-51 0.0 7.167379820958852E-5 0.0 0.4978641208133543 3.583689910479426E-5 52-53 0.0 7.167379820958852E-5 0.0 0.5824929580493259 3.583689910479426E-5 54-55 0.0 7.167379820958852E-5 0.0 0.6748984023910379 3.583689910479426E-5 56-57 0.0 7.167379820958852E-5 0.0 0.783412532880355 3.583689910479426E-5 58-59 0.0 7.167379820958852E-5 0.0 0.9072290192874191 3.583689910479426E-5 60-61 0.0 7.167379820958852E-5 0.0 1.0441976476659427 3.583689910479426E-5 62-63 0.0 7.167379820958852E-5 0.0 1.1836569405322497 3.583689910479426E-5 64-65 0.0 7.167379820958852E-5 0.0 1.3232954178940806 3.583689910479426E-5 66-67 0.0 7.167379820958852E-5 0.0 1.485439467893722 3.583689910479426E-5 68-69 0.0 7.167379820958852E-5 0.0 1.6710208499078991 3.583689910479426E-5 70-71 0.0 7.167379820958852E-5 0.0 1.880720465019603 3.583689910479426E-5 72-73 0.0 7.167379820958852E-5 0.0 2.094505486629253 3.583689910479426E-5 74-75 0.0 7.167379820958852E-5 0.0 2.3119996272962493 3.583689910479426E-5 76-77 0.0 7.167379820958852E-5 0.0 2.5481110370481863 3.583689910479426E-5 78-79 0.0 7.167379820958852E-5 0.0 2.8195217924183456 3.583689910479426E-5 80-81 0.0 7.167379820958852E-5 0.0 3.1179714881630725 3.583689910479426E-5 82-83 0.0 7.167379820958852E-5 0.0 3.4185713978540866 3.583689910479426E-5 84-85 0.0 7.167379820958852E-5 0.0 3.7217874011797507 3.583689910479426E-5 86-87 0.0 7.167379820958852E-5 0.0 4.051092667053705 3.583689910479426E-5 88-89 0.0 7.167379820958852E-5 0.0 4.398334300929609 3.583689910479426E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 9800 0.0 27.069086 1 TACATGG 10050 0.0 26.065659 2 ACATGGG 10690 0.0 23.883625 3 GAGTACT 7575 0.0 22.144377 12-13 GTATCAA 19740 0.0 21.46803 1 GTACTTT 8170 0.0 20.358877 14-15 CATGGGA 6730 0.0 20.025858 4 AGTACTT 8085 0.0 19.749756 12-13 TATCAAC 21980 0.0 19.129416 2 CAACGCA 21985 0.0 19.03838 5 ATCAACG 21985 0.0 19.017136 3 TCAACGC 22010 0.0 18.973633 4 AACGCAG 22290 0.0 18.839039 6 CATGGGG 4775 0.0 18.584723 4 GTATAGG 810 0.0 17.576164 1 ACGCAGA 23470 0.0 17.508064 7 AGTACAT 7395 0.0 17.455263 2 CGCAGAG 23445 0.0 17.385998 8 ACTTTTT 10115 0.0 17.149221 16-17 ATAGCGT 170 0.0018013641 16.746647 6 >>END_MODULE