FastQCFastQC Report
Fri 27 May 2016
SRR939645_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939645_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2790420
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96050.34421341590154886No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68100.2440492829036489No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53240.19079565083392466No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA53040.19007891285182876No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA44500.15947420101633447No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT29550.10589803685466703No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT28410.10181263035672049No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG105750.024.0444971
TACATGG104250.023.8893282
ACATGGG106450.022.1556853
GTATCAA182600.020.88751
GAGTACT76900.020.35542712-13
AGTACTT82900.019.76107612-13
CATGGGA67300.019.0259784
TCAACGC205750.018.3954034
AACGCAG205850.018.3369466
ATCAACG206100.018.3184833
CAACGCA207650.018.1817465
GTACTTT85450.018.0986514-15
GGTATCA141950.017.9458541
AGTACAT82350.017.8939022
TATCAAC212650.017.7776572
CATGGGG45600.017.2402974
CGCAGAG218800.016.843458
ACGCAGA222200.016.7338077
ATGGGAT30400.015.9498575
ACTTTTT99100.015.81948416-17
Produced by FastQC (version 0.11.5)