##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939645_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2790420 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.538412138674463 31.0 31.0 34.0 30.0 34.0 2 31.7440152378495 31.0 31.0 34.0 30.0 34.0 3 31.792197590326904 31.0 31.0 34.0 30.0 34.0 4 35.57866808580787 37.0 35.0 37.0 33.0 37.0 5 35.30504547702496 37.0 35.0 37.0 33.0 37.0 6 35.325676421470604 37.0 35.0 37.0 32.0 37.0 7 35.25421334422775 37.0 35.0 37.0 32.0 37.0 8 35.2163090144136 37.0 35.0 37.0 32.0 37.0 9 36.82923395044474 39.0 37.0 39.0 32.0 39.0 10-11 36.70823030224841 39.0 35.0 39.0 32.0 39.0 12-13 36.654360454698576 39.0 35.0 39.0 32.0 39.0 14-15 37.70537876018664 39.5 36.5 41.0 32.0 41.0 16-17 37.671752818572116 39.0 36.0 41.0 32.0 41.0 18-19 37.65575988560862 39.0 36.5 41.0 32.0 41.0 20-21 37.590513076884484 39.0 36.0 41.0 32.0 41.0 22-23 37.458365765727024 39.0 36.0 41.0 32.0 41.0 24-25 37.32982257151253 39.0 36.0 41.0 31.5 41.0 26-27 37.059699615111704 39.0 36.0 40.0 31.0 41.0 28-29 36.86199944811175 39.0 36.0 40.0 30.5 41.0 30-31 36.63721626135134 38.5 35.5 40.0 30.0 41.0 32-33 36.47771195733975 38.0 35.0 40.0 30.0 41.0 34-35 36.47652718945535 38.0 35.0 40.0 30.0 41.0 36-37 36.38042176446557 38.0 35.0 40.0 30.0 41.0 38-39 36.41411472108142 38.5 35.0 40.0 30.0 41.0 40-41 36.31798206004831 39.0 35.0 40.0 30.0 41.0 42-43 36.15004837981379 38.0 35.0 40.0 30.0 41.0 44-45 35.951666415808376 38.0 35.0 40.0 29.0 41.0 46-47 35.67494051074749 38.0 35.0 40.0 28.0 41.0 48-49 35.393519075981395 38.0 34.0 40.0 27.0 41.0 50-51 35.152441926305 38.0 34.0 40.0 26.5 41.0 52-53 34.90343550433268 38.0 34.0 40.0 26.0 41.0 54-55 34.626065610194885 38.0 33.5 40.0 26.0 41.0 56-57 32.58393772263673 37.0 30.5 39.5 16.0 41.0 58-59 27.479825796833453 32.5 17.5 38.5 7.0 40.0 60-61 28.216885271751206 33.0 20.0 38.0 9.0 40.0 62-63 31.354684778635473 34.5 29.5 38.5 12.5 40.0 64-65 31.590802997398242 35.5 30.5 39.0 7.0 40.0 66-67 24.848038646511995 27.0 18.0 35.5 2.0 39.0 68-69 17.371088581647207 13.0 7.5 28.0 2.0 35.5 70-71 16.24106604023767 12.5 7.5 26.5 2.0 33.5 72-73 17.323671705334682 13.5 7.0 28.5 2.0 34.0 74-75 18.307119179191663 17.0 8.5 29.0 2.0 34.5 76-77 19.509929150450468 21.0 8.0 30.0 2.0 34.5 78-79 22.569728392141684 27.5 10.5 33.5 2.0 35.5 80-81 26.268647192895692 31.5 22.5 34.5 2.0 36.0 82-83 27.604949792504353 33.0 26.5 35.0 2.0 36.0 84-85 27.6613651349976 34.0 27.0 35.0 2.0 36.0 86-87 27.419464632564274 34.0 27.0 35.0 2.0 36.0 88-89 26.98849241332846 33.0 26.0 35.0 2.0 35.5 90-91 25.591244866364203 32.5 17.5 35.0 2.0 35.0 92-93 23.069832856702575 31.5 2.0 34.5 2.0 35.0 94-95 15.730804860916994 4.5 2.0 32.0 2.0 35.0 96-97 13.163296923043843 2.0 2.0 30.0 2.0 34.0 98-99 12.832427018154974 2.0 2.0 29.5 2.0 34.0 100-101 12.538491338221487 2.0 2.0 30.5 2.0 34.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.4397285789968066 1101 2 0.4661899768036726 1101 3 0.46875203404632515 1101 4 0.37370869956623665 1101 5 0.4581737793101013 1101 6 0.4397315008187235 1101 7 0.4506224033025177 1101 8 0.45199825727978293 1101 9 0.5007600726620751 1101 10-11 0.5062730807566851 1101 12-13 0.5320173497735325 1101 14-15 0.6440082294896499 1101 16-17 0.6542244221636437 1101 18-19 0.581577439684466 1101 20-21 0.6021641526122892 1101 22-23 0.6765821443182247 1101 24-25 0.6251299640395018 1101 26-27 0.7859062487043786 1101 28-29 0.6754754802982745 1101 30-31 0.7273813599009671 1101 32-33 0.8266006407040933 1101 34-35 0.6248845409555557 1101 36-37 0.4689569479867899 1101 38-39 0.5964091544733563 1101 40-41 0.7221888356323305 1101 42-43 0.6916830279151114 1101 44-45 0.5636629520999605 1101 46-47 0.5489158856506577 1101 48-49 0.523745645165242 1101 50-51 0.4046297012768605 1101 52-53 0.41581040382139633 1101 54-55 0.35854073563061917 1101 56-57 -1.9511239720455684 1101 58-59 -9.983149338288772 1101 60-61 -10.148329348568506 1101 62-63 -3.799925066569603 1101 64-65 -5.117120896539518 1101 66-67 -9.933409808945875 1101 68-69 -5.033768471857773 1101 70-71 -4.474991527075078 1101 72-73 -4.552285553535558 1101 74-75 -6.742716755700947 1101 76-77 -7.842999842514409 1101 78-79 -11.00022174147124 1101 80-81 -7.930201223625353 1101 82-83 -4.149330846228921 1101 84-85 -3.2679804669780204 1101 86-87 -3.045047313829201 1101 88-89 -3.228194617297447 1101 90-91 -9.347657153034994 1101 92-93 -17.011214574245408 1101 94-95 -11.123798267592097 1101 96-97 -9.141246394103721 1101 98-99 -9.107965032259553 1101 100-101 -9.084415594548686 1102 1 0.39731642223381414 1102 2 0.4388001346373436 1102 3 0.41737058200350674 1102 4 0.2955645292522746 1102 5 0.32756061772529677 1102 6 0.34083642269234815 1102 7 0.4214952331410231 1102 8 0.45693807548877885 1102 9 0.5382324112160717 1102 10-11 0.5436679653887424 1102 12-13 0.49056180045759135 1102 14-15 0.6039146329341136 1102 16-17 0.6006522510299988 1102 18-19 0.5169869564581546 1102 20-21 0.5666823892178172 1102 22-23 0.57361900264479 1102 24-25 0.567826986399794 1102 26-27 0.5056817990393156 1102 28-29 0.662765263610865 1102 30-31 0.6109277492744738 1102 32-33 0.6087579731273323 1102 34-35 0.35892051029467353 1102 36-37 0.42984711926204255 1102 38-39 0.4140814983470449 1102 40-41 0.33969879787377977 1102 42-43 0.5567527701612036 1102 44-45 0.6453615737385832 1102 46-47 0.5266112165746435 1102 48-49 0.5286815298014247 1102 50-51 0.483693859348044 1102 52-53 0.32486367151525997 1102 54-55 0.165646004407904 1102 56-57 -2.3958279594521414 1102 58-59 -10.295873996807824 1102 60-61 -10.668619466422093 1102 62-63 -4.251299831538628 1102 64-65 -5.0669082775629875 1102 66-67 -6.593106684078162 1102 68-69 -4.033628642709697 1102 70-71 -3.905028158003674 1102 72-73 -4.1210465605152145 1102 74-75 -5.187457831079815 1102 76-77 -7.570903746454313 1102 78-79 -9.825165294701284 1102 80-81 -5.562351194648034 1102 82-83 -2.8713707296603026 1102 84-85 -2.5143754411456847 1102 86-87 -2.444574404719834 1102 88-89 -2.7346551360270652 1102 90-91 -8.89318779013518 1102 92-93 -13.41486554795453 1102 94-95 -10.789032676688915 1102 96-97 -9.40375243963052 1102 98-99 -9.45742523368096 1102 100-101 -9.332703741120351 1103 1 0.28181158093477876 1103 2 0.22524361494594203 1103 3 0.1939809061005846 1103 4 0.17221687726989643 1103 5 0.24713830489857003 1103 6 0.2501871862096223 1103 7 0.23260901593912564 1103 8 0.27294610084431525 1103 9 0.4018132242631154 1103 10-11 0.3931765557509266 1103 12-13 0.3733208528347163 1103 14-15 0.580617001550074 1103 16-17 0.5302554283031142 1103 18-19 0.48157808080760134 1103 20-21 0.3841205790346791 1103 22-23 0.4650865750133448 1103 24-25 0.4834112981312586 1103 26-27 0.4813781028645323 1103 28-29 0.5223256779600973 1103 30-31 0.49984782827062446 1103 32-33 0.45637286746364936 1103 34-35 0.3801912596054464 1103 36-37 0.20397888686424182 1103 38-39 0.16600814620036886 1103 40-41 0.3342417546481542 1103 42-43 0.322860560965907 1103 44-45 0.2794468337657321 1103 46-47 0.3520371223914296 1103 48-49 0.24812360434921032 1103 50-51 0.2599498478945961 1103 52-53 0.3744350771602498 1103 54-55 0.14123187135532334 1103 56-57 -2.6948135395118484 1103 58-59 -10.200680992853055 1103 60-61 -10.538531580175661 1103 62-63 -4.31878227883087 1103 64-65 -5.2252049962227645 1103 66-67 -6.500911882801123 1103 68-69 -4.216650537613496 1103 70-71 -4.285009467837437 1103 72-73 -4.418617969469178 1103 74-75 -5.173766277832694 1103 76-77 -6.731849571443988 1103 78-79 -9.650396109154645 1103 80-81 -5.357541937473222 1103 82-83 -3.000176665732102 1103 84-85 -2.4172943939889997 1103 86-87 -2.27887400328661 1103 88-89 -2.256354259094831 1103 90-91 -5.406373346701198 1103 92-93 -10.002904282758774 1103 94-95 -8.507812681464861 1103 96-97 -7.919290811826787 1103 98-99 -8.067894925444879 1103 100-101 -8.068216104811464 1104 1 0.25987533895220594 1104 2 0.30927550683922433 1104 3 0.197712793613114 1104 4 0.20555441956350506 1104 5 0.3124275248923354 1104 6 0.2813098705298742 1104 7 0.3163626010713614 1104 8 0.21646760906080686 1104 9 0.2713041032744101 1104 10-11 0.3976743022392384 1104 12-13 0.5077619587433304 1104 14-15 0.5574209060908544 1104 16-17 0.42420199855882146 1104 18-19 0.4457312680642431 1104 20-21 0.4398994385438826 1104 22-23 0.44248793717031987 1104 24-25 0.5017689790113238 1104 26-27 0.5486603084971549 1104 28-29 0.49386082320788205 1104 30-31 0.4575163391490662 1104 32-33 0.6411022012539505 1104 34-35 0.6168909021874072 1104 36-37 0.4890473425967201 1104 38-39 0.48514895138306713 1104 40-41 0.3752092829491218 1104 42-43 0.4681147864613777 1104 44-45 0.6027176597441155 1104 46-47 0.5363559537091831 1104 48-49 0.5353232971810087 1104 50-51 0.5804269120675016 1104 52-53 0.5339713356350799 1104 54-55 0.5693631445314438 1104 56-57 -2.382521657565455 1104 58-59 -10.050746704171633 1104 60-61 -10.247414461990633 1104 62-63 -3.9824363177577418 1104 64-65 -4.934638178119428 1104 66-67 -6.606456758477886 1104 68-69 -4.111389766755307 1104 70-71 -3.9618042243319067 1104 72-73 -4.281042820201407 1104 74-75 -4.856920472662889 1104 76-77 -6.535510078426135 1104 78-79 -9.098414461939388 1104 80-81 -4.825775668768042 1104 82-83 -2.6187022325888556 1104 84-85 -2.111451627001337 1104 86-87 -1.8582171993305288 1104 88-89 -2.028845181913667 1104 90-91 -4.689390965065318 1104 92-93 -6.7116051055675605 1104 94-95 -7.751147363383426 1104 96-97 -7.787918037958761 1104 98-99 -8.30211222633314 1104 100-101 -8.373611390604161 1105 1 0.3182769905856553 1105 2 0.31295799613053177 1105 3 0.30558854851703643 1105 4 0.27926928056420763 1105 5 0.3163648281515776 1105 6 0.31490780037373156 1105 7 0.3006246166919553 1105 8 0.31728679067030896 1105 9 0.37614148551705284 1105 10-11 0.44139607907222 1105 12-13 0.3749458879823706 1105 14-15 0.45513230151951944 1105 16-17 0.5762824263706747 1105 18-19 0.4948205984151812 1105 20-21 0.4256822354748877 1105 22-23 0.5357880013523086 1105 24-25 0.5342643312402799 1105 26-27 0.6145254446637693 1105 28-29 0.6929317155926782 1105 30-31 0.645631187900328 1105 32-33 0.6193189245530348 1105 34-35 0.7505555021856196 1105 36-37 0.5628862292242829 1105 38-39 0.429231321242284 1105 40-41 0.5911678211310161 1105 42-43 0.5894991268224103 1105 44-45 0.5295256969109801 1105 46-47 0.6405348645038842 1105 48-49 0.46077330477310596 1105 50-51 0.5174876067896506 1105 52-53 0.5862644061497733 1105 54-55 0.5773859565700903 1105 56-57 1.611184616064179 1105 58-59 -8.647764666195805 1105 60-61 -9.851046103326897 1105 62-63 -3.543040764050943 1105 64-65 -4.849399715884083 1105 66-67 -6.347288942362464 1105 68-69 -3.9320044974780615 1105 70-71 -4.127283566791927 1105 72-73 -2.376000195962053 1105 74-75 -4.209127733265996 1105 76-77 -6.533112887632104 1105 78-79 -9.164914846648507 1105 80-81 -4.535991450391069 1105 82-83 -2.105501414428037 1105 84-85 -1.6046316211062894 1105 86-87 -1.4340055340312894 1105 88-89 -1.5325351357102939 1105 90-91 -4.223523659726222 1105 92-93 -6.739941388550145 1105 94-95 -8.100832897172115 1105 96-97 -8.040079682140467 1105 98-99 -8.575006693539718 1105 100-101 -8.614098293268288 1106 1 0.15914872915027445 1106 2 0.08131667791723629 1106 3 0.10554758246984974 1106 4 0.10808021016895708 1106 5 0.08489733315967385 1106 6 0.1735794046884891 1106 7 0.13486663511196184 1106 8 0.1455356524435345 1106 9 0.14056910851012105 1106 10-11 0.16678810430457247 1106 12-13 0.24421965771666265 1106 14-15 0.27125439873412205 1106 16-17 0.2617988396579136 1106 18-19 0.26427148915079357 1106 20-21 0.23754389005911491 1106 22-23 0.2100257444831115 1106 24-25 0.28795426451081596 1106 26-27 0.3615610229794086 1106 28-29 0.32905086714090714 1106 30-31 0.40486034038008256 1106 32-33 0.27286966786081024 1106 34-35 0.3125065318615654 1106 36-37 0.3102685999759416 1106 38-39 0.32170523560590425 1106 40-41 0.3224859306333414 1106 42-43 0.22179771491274636 1106 44-45 0.1434927015059273 1106 46-47 0.2307748679934818 1106 48-49 0.14233067412222766 1106 50-51 0.3030873054435119 1106 52-53 0.3800858249282797 1106 54-55 0.19134093937762486 1106 56-57 1.4529525361806463 1106 58-59 -8.78237229147761 1106 60-61 -10.253867557710915 1106 62-63 -4.019037721684718 1106 64-65 -5.582247877030163 1106 66-67 -7.32665180339546 1106 68-69 -4.267347002143239 1106 70-71 -4.419485788759845 1106 72-73 -4.630049534691484 1106 74-75 -5.117809513399722 1106 76-77 -6.936179310569621 1106 78-79 -9.457084747745327 1106 80-81 -5.185372538555971 1106 82-83 -2.6629852661456574 1106 84-85 -2.1927180823269588 1106 86-87 -2.014296818655847 1106 88-89 -1.9708629063939753 1106 90-91 -4.384820704166497 1106 92-93 -6.8398994633876775 1106 94-95 -7.915093850892701 1106 96-97 -7.7405447610815665 1106 98-99 -7.090117883248648 1106 100-101 -7.541563295086009 1107 1 0.11191929742767215 1107 2 -0.07073912140065275 1107 3 -0.017988593611146797 1107 4 0.03348083582912409 1107 5 0.006195157145761243 1107 6 0.03660400263886032 1107 7 0.0044442733619050045 1107 8 0.11277981749478982 1107 9 0.17262371802311804 1107 10-11 0.14728524022902434 1107 12-13 0.15613011097536145 1107 14-15 0.16842589075578474 1107 16-17 0.12844664665130523 1107 18-19 0.17776742071422547 1107 20-21 0.221960483786944 1107 22-23 0.15724770828139611 1107 24-25 0.14326892676761105 1107 26-27 0.1167363261739709 1107 28-29 0.1829102334685473 1107 30-31 0.25070744665433864 1107 32-33 0.2056560706692565 1107 34-35 0.06878335389270518 1107 36-37 0.15912521646195188 1107 38-39 -0.02230868511131945 1107 40-41 -0.0038714120766698557 1107 42-43 0.04798190303861816 1107 44-45 0.034829719437169615 1107 46-47 -0.03218872081247781 1107 48-49 -0.27270871284978426 1107 50-51 -0.10716735710440872 1107 52-53 -0.17840397091259064 1107 54-55 -0.2058392230307433 1107 56-57 1.2178652532330148 1107 58-59 -8.94496393968015 1107 60-61 -10.199418162074839 1107 62-63 -3.9693646332360153 1107 64-65 -5.4382660430725025 1107 66-67 -6.866332049659761 1107 68-69 -3.959518212535553 1107 70-71 -3.9706312607611416 1107 72-73 -2.443571477415034 1107 74-75 -4.867463868750143 1107 76-77 -6.982475947915194 1107 78-79 -7.478989941813264 1107 80-81 -2.535812608146898 1107 82-83 -1.3057974206354963 1107 84-85 -1.0415163477433218 1107 86-87 -1.0706481300676565 1107 88-89 -0.9239063070588713 1107 90-91 -0.3511860590732745 1107 92-93 -3.2999916352942336 1107 94-95 -5.868078014463027 1107 96-97 -6.647475564434032 1107 98-99 -6.390462800092045 1107 100-101 -7.167895006360865 1108 1 -0.04318455094042406 1108 2 -0.009342371921000847 1108 3 -0.012291768846964146 1108 4 0.008339345195402359 1108 5 -0.03802963360752898 1108 6 -0.15068088288436599 1108 7 -0.10664558094038057 1108 8 -0.10910406718702603 1108 9 -0.11915609686550965 1108 10-11 -0.05851786857927266 1108 12-13 -0.027650943893767987 1108 14-15 -0.009180061150679819 1108 16-17 -0.07059679695172605 1108 18-19 -0.07878100495337037 1108 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2316 90-91 2.8646347074287632 2316 92-93 5.03515578448625 2316 94-95 11.949347405939427 2316 96-97 13.807614858770812 2316 98-99 13.680323643718715 2316 100-101 12.480274479387353 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 22.0 4 29.0 5 86.0 6 244.0 7 579.0 8 1345.0 9 2355.0 10 3845.0 11 6197.0 12 9346.0 13 13123.0 14 16139.0 15 22461.0 16 30215.0 17 41063.0 18 45894.0 19 47029.0 20 49075.0 21 49513.0 22 48902.0 23 41604.0 24 42251.0 25 49574.0 26 70044.0 27 104624.0 28 147995.0 29 199435.0 30 247942.0 31 269555.0 32 268689.0 33 244778.0 34 217170.0 35 194863.0 36 158628.0 37 102856.0 38 40033.0 39 2915.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.312571353809155 19.785468107871726 14.273199285252142 23.628761253066983 2 15.309793442366788 21.39287524182733 39.70369297838804 23.593638337417847 3 18.660183514119062 25.951006518033896 30.46768720891395 24.92112275893309 4 12.622759297883473 17.452426516438386 38.078138774808096 31.846675410870045 5 13.086917855366357 38.40731587745812 34.886652134060775 13.619114133114746 6 30.872270124210694 37.61423011589653 17.88286351158607 13.630636248306708 7 27.460346471140546 33.18747715397682 21.848395582027077 17.503780792855554 8 25.549350913709368 35.67949787556121 20.33910028153831 18.432050929191107 9 26.302922140753004 16.44738068104443 20.502576673045635 36.74712050515693 10-11 24.25385784218863 26.729703772192 28.439894352821437 20.57654403279793 12-13 25.659775230968812 24.165914091785464 28.44496527404477 21.729345403200952 14-15 22.587223590305406 25.52165898766145 26.708155334691202 25.18296208734194 16-17 21.871187849857726 28.562259444814757 27.391199174317844 22.17535353100967 18-19 21.755716710339964 27.674386184712247 29.248560071329866 21.321337033617926 20-21 22.635409723267465 26.886543961124133 28.90005805577655 21.577988259831855 22-23 22.24173150182231 26.83770485340864 28.623069728104472 22.297493916664575 24-25 22.305620659255595 26.943470875352098 28.811146709097557 21.93976175629475 26-27 22.303395036896887 26.96045009848906 28.879898179892106 21.85625668472195 28-29 22.00519258416267 27.05646706808937 29.051861512214256 21.886478835533705 30-31 22.160737397353216 27.16315851914084 28.817693292793734 21.85841079071221 32-33 22.075153484714964 27.10354963529573 28.696167460416156 22.12512941957315 34-35 22.442120149396953 26.945693172147855 28.63876425504281 21.97342242341238 36-37 22.504013249958426 26.780615639449874 28.77750908172141 21.937862028870285 38-39 22.388511906342178 26.89376028310883 28.925089099348554 21.792638711200436 40-41 22.39069027288523 26.764981059229935 28.959347536892565 21.88498113099227 42-43 22.106875767424803 27.09812416439953 28.58953869471581 22.20546137345986 44-45 22.228136535602133 27.262682345039007 28.311363662458593 22.197817456900264 46-47 22.350597709619933 27.17794736911973 27.892063175425864 22.579391745834474 48-49 22.42005133419393 27.170599011346763 28.06557487623896 22.34377477822035 50-51 22.43728170805431 27.337479572268876 28.045036980696132 22.180201738980685 52-53 22.47070365043097 27.440824422771342 27.893711731811514 22.19476019498617 54-55 22.189292397299027 27.220346794273475 28.05951039226182 22.53085041616568 56-57 21.452835420182456 27.50626829434833 28.827902372970765 22.21299391249845 58-59 21.00370526010802 26.9029838454736 29.877128922995784 22.216181971422596 60-61 22.225033877950047 27.062106333177482 28.53150163068027 22.181358158192197 62-63 22.63339200104935 27.082049865049918 27.998925560093912 22.28563257380682 64-65 21.922608633097912 27.21877993093651 28.41347895185235 22.445132484113227 66-67 21.56552332621941 27.05191776479645 29.137469634356016 22.24508927462813 68-69 21.79681196743823 26.718818448522285 29.46802453785033 22.016345046189155 70-71 21.66936129036893 26.64991744817508 29.29071007832246 22.39001118313353 72-73 20.930316820644133 26.78816145002134 29.718132127140244 22.563389602194277 74-75 21.486086480641273 27.043451621735937 28.964262696253208 22.506199201369583 76-77 19.94493350447204 27.37832895553543 29.662845360285257 23.01389217970727 78-79 20.65148216288302 27.339340799171275 29.06793854654339 22.941238491402313 80-81 21.638396261831026 27.624536610254935 27.865988707614562 22.871078420299472 82-83 22.12816617160915 27.51438718915259 27.436370161945355 22.921076477292907 84-85 22.14056178700173 27.22098525059158 27.362594909649708 23.27585805275699 86-87 22.126921560051155 27.24893147365341 27.621959293773653 23.002187672521785 88-89 22.320893257172674 27.015209554700963 27.564553971682372 23.099343216443994 90-91 21.472009139432934 27.28735056137997 28.145573491333437 23.095066807853662 92-93 20.41133420188901 27.56089168278495 28.75837249958224 23.2694016157438 94-95 19.577774414828557 27.499091638172985 29.18051141684706 23.7426225301514 96-97 19.586167451871304 27.510674004584025 28.86279106520518 24.040367478339494 98-99 19.90319020948804 27.35895049014522 28.55834308039312 24.17951621997362 100-101 20.566125128885535 27.321725602088286 28.121977938305314 23.990171330720866 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 492.0 1 385.5 2 292.5 3 338.5 4 397.0 5 454.0 6 635.5 7 919.0 8 1207.0 9 1450.0 10 1610.5 11 1809.0 12 2081.0 13 2343.0 14 2578.0 15 2859.5 16 3119.0 17 3374.0 18 3747.0 19 4199.0 20 4788.0 21 5539.0 22 6498.5 23 7887.0 24 9623.5 25 11954.0 26 15196.0 27 18985.0 28 23213.0 29 28110.5 30 33548.0 31 39774.5 32 46621.0 33 53558.0 34 60632.0 35 67760.0 36 74289.0 37 79789.0 38 84528.5 39 88751.0 40 91976.0 41 94615.0 42 97767.5 43 101209.0 44 104717.5 45 108319.5 46 112082.5 47 115766.5 48 118299.5 49 115348.0 50 108499.0 51 102725.5 52 97008.0 53 91359.0 54 85508.0 55 78543.0 56 70580.5 57 62000.0 58 53284.0 59 44473.5 60 36145.0 61 28518.0 62 21991.5 63 16629.0 64 12111.0 65 8624.0 66 5959.5 67 3991.0 68 2639.5 69 1689.5 70 1069.0 71 700.5 72 442.0 73 312.5 74 193.0 75 103.0 76 61.0 77 31.5 78 17.0 79 6.5 80 3.5 81 3.0 82 3.5 83 2.0 84 0.0 85 0.0 86 0.0 87 0.5 88 1.0 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.022398061940496414 2 0.0073824012155876174 3 2.8669519283835407E-4 4 0.0 5 0.1448527461815784 6 0.0 7 0.0 8 0.0012901283677725934 9 0.0 10-11 0.0 12-13 0.0 14-15 6.988195325434881E-4 16-17 0.0 18-19 1.4334759641917703E-4 20-21 0.0 22-23 3.5836899104794257E-4 24-25 0.0 26-27 3.046136423907512E-4 28-29 0.0033865869654030574 30-31 0.014012227549974556 32-33 0.0027594412310691582 34-35 0.004855899828699622 36-37 0.11075393668336665 38-39 0.11044932304097592 40-41 0.12336852516825424 42-43 0.1255008206649895 44-45 0.12170210935988132 46-47 0.11457056643802725 48-49 0.11446305574071287 50-51 0.1130654166756259 52-53 0.09066735473512948 54-55 0.07767647880964156 56-57 0.5426960815934518 58-59 1.5449108019581281 60-61 0.5116792454182524 62-63 0.00462295998451846 64-65 0.1173479261186488 66-67 1.8681058765347152 68-69 2.8142000129012836 70-71 2.725915811956623 72-73 2.5764759426896306 74-75 1.970563571075322 76-77 1.385311171794927 78-79 0.9207753671490314 80-81 0.05235770959210441 82-83 1.254291468667799E-4 84-85 1.0751069731438278E-4 86-87 4.1212433970513396E-4 88-89 1.0751069731438278E-4 90-91 0.24646827359322251 92-93 0.38379527096279414 94-95 1.9612101404089706 96-97 2.3794088345123674 98-99 1.388160205273758 100-101 0.9265451079049032 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2790420.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.256952233184236 #Duplication Level Percentage of deduplicated Percentage of total 1 69.56097884888557 32.87239854755324 2 15.07267365832175 14.245772381953703 3 6.104709818142063 8.654699408201708 4 2.9210946230195916 5.521681162745926 5 1.689438166009007 3.991884935600301 6 1.0818920365783498 3.0676152176427296 7 0.7055149425131142 2.3338340156697974 8 0.5144748982131321 1.9450012552024245 9 0.38155956279631387 1.6228207829862058 >10 1.7981252373108305 14.927675421682116 >50 0.1132261825690599 3.6825640879691965 >100 0.052665007539553 4.439049003552721 >500 0.0022793863135646968 0.7527612543975745 >1k 0.0010637136129685349 0.9712469320797305 >5k 3.039181751338671E-4 0.9709955927627414 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9605 0.34421341590154886 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6810 0.2440492829036489 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5324 0.19079565083392466 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 5304 0.19007891285182876 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 4450 0.15947420101633447 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 2955 0.10589803685466703 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2841 0.10181263035672049 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 3.583689910479426E-5 0.0 10-11 0.0 0.0 0.0 3.583689910479426E-5 0.0 12-13 0.0 0.0 0.0 3.583689910479426E-5 0.0 14-15 0.0 0.0 0.0 5.375534865719139E-5 0.0 16-17 0.0 0.0 0.0 7.167379820958852E-5 0.0 18-19 0.0 0.0 0.0 1.4334759641917703E-4 0.0 20-21 0.0 0.0 0.0 2.5085829373355984E-4 0.0 22-23 0.0 0.0 0.0 4.3004278925753113E-4 0.0 24-25 0.0 0.0 0.0 7.525748812006795E-4 0.0 26-27 0.0 0.0 0.0 0.0011646992209058135 0.0 28-29 0.0 0.0 0.0 0.002920707277040732 0.0 30-31 0.0 0.0 0.0 0.009263838418589316 0.0 32-33 0.0 0.0 0.0 0.022057611399000865 0.0 34-35 0.0 0.0 0.0 0.0400118978505028 0.0 36-37 0.0 0.0 0.0 0.06918313372180532 0.0 38-39 0.0 0.0 0.0 0.11385382845593137 0.0 40-41 0.0 0.0 0.0 0.16544104471728271 0.0 42-43 0.0 0.0 0.0 0.21752997756610115 0.0 44-45 0.0 0.0 0.0 0.27748510976842194 0.0 46-47 0.0 0.0 0.0 0.34371169931408174 0.0 48-49 0.0 0.0 0.0 0.409508246070484 0.0 50-51 3.583689910479426E-5 0.0 0.0 0.4733337633761226 0.0 52-53 3.583689910479426E-5 1.791844955239713E-5 0.0 0.5378939371134095 0.0 54-55 3.583689910479426E-5 3.583689910479426E-5 0.0 0.6084388730011969 0.0 56-57 3.583689910479426E-5 3.583689910479426E-5 0.0 0.6662617097067824 0.0 58-59 3.583689910479426E-5 3.583689910479426E-5 0.0 0.7073487145304291 0.0 60-61 3.583689910479426E-5 1.0751069731438278E-4 0.0 0.7389389410913052 0.0 62-63 1.0751069731438278E-4 1.0751069731438278E-4 0.0 0.769561571376352 0.0 64-65 1.0751069731438278E-4 1.0751069731438278E-4 0.0 0.8000946094136366 0.0 66-67 1.0751069731438278E-4 1.0751069731438278E-4 0.0 0.8252879494843071 0.0 68-69 1.4334759641917703E-4 1.0751069731438278E-4 0.0 0.8627195905992646 0.0 70-71 1.6126604597157417E-4 1.4334759641917703E-4 0.0 0.9216354527275463 0.0 72-73 1.791844955239713E-4 1.4334759641917703E-4 0.0 1.003146479741401 0.0 74-75 1.791844955239713E-4 1.4334759641917703E-4 0.0 1.107736469778743 0.0 76-77 1.791844955239713E-4 1.4334759641917703E-4 0.0 1.2715290171372051 0.0 78-79 1.791844955239713E-4 1.4334759641917703E-4 0.0 1.5270102708552835 0.0 80-81 1.791844955239713E-4 1.4334759641917703E-4 0.0 1.8377161860938496 0.0 82-83 1.791844955239713E-4 1.4334759641917703E-4 0.0 2.148887981020778 0.0 84-85 1.791844955239713E-4 1.4334759641917703E-4 0.0 2.390858723776349 0.0 86-87 1.791844955239713E-4 1.4334759641917703E-4 0.0 2.5776585603600894 0.0 88-89 1.791844955239713E-4 1.4334759641917703E-4 0.0 2.7508045383849025 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 10575 0.0 24.044497 1 TACATGG 10425 0.0 23.889328 2 ACATGGG 10645 0.0 22.155685 3 GTATCAA 18260 0.0 20.8875 1 GAGTACT 7690 0.0 20.355427 12-13 AGTACTT 8290 0.0 19.761076 12-13 CATGGGA 6730 0.0 19.025978 4 TCAACGC 20575 0.0 18.395403 4 AACGCAG 20585 0.0 18.336946 6 ATCAACG 20610 0.0 18.318483 3 CAACGCA 20765 0.0 18.181746 5 GTACTTT 8545 0.0 18.09865 14-15 GGTATCA 14195 0.0 17.945854 1 AGTACAT 8235 0.0 17.893902 2 TATCAAC 21265 0.0 17.777657 2 CATGGGG 4560 0.0 17.240297 4 CGCAGAG 21880 0.0 16.84345 8 ACGCAGA 22220 0.0 16.733807 7 ATGGGAT 3040 0.0 15.949857 5 ACTTTTT 9910 0.0 15.819484 16-17 >>END_MODULE