FastQCFastQC Report
Fri 27 May 2016
SRR939644_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939644_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2735445
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36140.13211744341414286No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG83300.031.1542661
TACATGG100600.027.3355162
ACATGGG102200.026.0296153
GTATCAA164400.024.9989621
CATGGGA67050.022.688394
GAGTACT72700.020.97435212-13
TATCAAC201200.019.8205972
GTACTTT78200.019.71190814-15
AGTACTT77950.019.68283712-13
ATCAACG211300.018.9853423
CAACGCA212550.018.8782775
AACGCAG215550.018.8345386
TCAACGC212100.018.8218634
CATGGGG44700.018.8130424
ACGCAGA219800.018.1052467
CGCAGAG228150.017.3814988
GGTATCA131800.017.1617681
ATGGGAT31950.016.8437395
GTATAAT13900.016.7086931
GTACTAA8003.436071E-916.4090251
Produced by FastQC (version 0.11.5)