FastQCFastQC Report
Fri 27 May 2016
SRR939644_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939644_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2735445
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92480.3380802757869378No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68000.24858843807863074No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52270.19108408321132395No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA52060.19031638362313993No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA42140.15405171736225734No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT28360.10367600152808776No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT27500.10053208892885801No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG103500.024.8053781
TACATGG103550.024.1013772
ACATGGG102700.023.194543
GAGTACT75550.021.0931112-13
CATGGGA65950.020.6703534
GTATCAA178850.020.3975411
AGTACTT78700.019.67972212-13
GTACTTT82650.019.48077414-15
TCAACGC197050.018.216844
ATCAACG197100.018.2122173
GGTATCA137850.018.179211
CAACGCA199100.018.005585
AACGCAG200500.017.8710336
TATCAAC202300.017.8169672
CATGGGG43450.017.0441254
ACGCAGA217400.016.3733627
CGCAGAG216200.016.2897858
ACTTTTT100300.016.19372216-17
AGTACAT79000.015.944852
GCAGAGT219700.015.5581669
Produced by FastQC (version 0.11.5)