##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939644_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2735445 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.473352964508518 31.0 31.0 34.0 30.0 34.0 2 31.67611558631228 31.0 31.0 34.0 30.0 34.0 3 31.729237473244755 31.0 31.0 34.0 30.0 34.0 4 35.5286547527002 37.0 35.0 37.0 33.0 37.0 5 35.28474123954238 37.0 35.0 37.0 33.0 37.0 6 35.286106282524415 37.0 35.0 37.0 32.0 37.0 7 35.19373155007686 37.0 35.0 37.0 32.0 37.0 8 35.15213722081782 37.0 35.0 37.0 32.0 37.0 9 36.74960966131653 39.0 35.0 39.0 32.0 39.0 10-11 36.62568211022338 39.0 35.0 39.0 32.0 39.0 12-13 36.5664418403587 39.0 35.0 39.0 32.0 39.0 14-15 37.629281159007036 39.0 36.0 41.0 32.0 41.0 16-17 37.57873307633676 39.0 36.0 41.0 32.0 41.0 18-19 37.55235455291552 39.0 36.0 41.0 32.0 41.0 20-21 37.49259279568772 39.0 36.0 41.0 32.0 41.0 22-23 37.35788326944976 39.0 36.0 41.0 31.5 41.0 24-25 37.230763550354695 39.0 36.0 40.5 31.0 41.0 26-27 36.93604166780908 39.0 36.0 40.0 30.5 41.0 28-29 36.739618782318786 39.0 35.5 40.0 30.0 41.0 30-31 36.51027511063099 38.0 35.0 40.0 30.0 41.0 32-33 36.35474776498887 38.0 35.0 40.0 30.0 41.0 34-35 36.33881543953543 38.0 35.0 40.0 30.0 41.0 36-37 36.26467265837917 38.0 35.0 40.0 30.0 41.0 38-39 36.31041878012535 38.0 35.0 40.0 30.0 41.0 40-41 36.20726993231449 38.0 35.0 40.0 30.0 41.0 42-43 36.045822343348156 38.0 35.0 40.0 29.5 41.0 44-45 35.84302773406155 38.0 35.0 40.0 28.5 41.0 46-47 35.570050942351244 38.0 34.5 40.0 27.5 41.0 48-49 35.298239408944426 38.0 34.0 40.0 27.0 41.0 50-51 35.07032841091669 38.0 34.0 40.0 26.0 41.0 52-53 34.831264565728794 38.0 33.5 40.0 26.0 41.0 54-55 34.56233208856329 38.0 33.0 40.0 25.5 41.0 56-57 33.764321709996 37.5 32.5 40.0 22.5 41.0 58-59 30.65425991017915 35.5 27.0 39.0 8.5 40.5 60-61 30.38722493049577 34.5 26.0 39.0 10.5 40.0 62-63 32.19986620092892 35.5 30.5 39.0 19.5 40.0 64-65 32.2467933005416 36.0 31.0 39.0 18.0 40.0 66-67 27.32883187196233 32.0 18.5 37.0 4.5 39.5 68-69 19.812986004105362 20.0 8.0 32.0 2.0 37.0 70-71 17.869784806494007 15.5 7.5 29.0 2.0 35.0 72-73 19.57767566154684 20.0 7.5 31.0 2.0 35.5 74-75 21.024594353021172 23.5 9.5 32.5 2.0 36.0 76-77 22.867053258245 27.5 11.5 33.0 2.0 35.5 78-79 25.05688909848306 30.5 13.0 34.5 2.0 36.5 80-81 26.67623659039023 32.0 22.5 35.0 2.0 37.0 82-83 27.815273383306923 33.0 26.5 35.0 2.0 36.0 84-85 27.862523830674718 34.0 27.0 35.0 2.0 36.0 86-87 27.577444986099152 33.5 27.0 35.0 2.0 36.0 88-89 27.127861463125747 33.0 26.0 35.0 2.0 35.5 90-91 25.284712176629398 32.0 16.0 34.5 2.0 35.0 92-93 23.65742758490849 31.0 2.0 34.0 2.0 35.0 94-95 19.892604493967163 28.0 2.0 34.0 2.0 35.0 96-97 17.188830336563157 18.5 2.0 33.0 2.0 35.0 98-99 17.46369493811793 21.5 2.0 33.0 2.0 35.0 100-101 16.67861682468483 10.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.24675260732371385 1101 2 0.23901405151825728 1101 3 0.24137168862218772 1101 4 0.15038629499878198 1101 5 0.33444034483302687 1101 6 0.2767232415190648 1101 7 0.34074964396933183 1101 8 0.38057589035866357 1101 9 0.3387404493493449 1101 10-11 0.28555398080957417 1101 12-13 0.32252022310696304 1101 14-15 0.3906014433961573 1101 16-17 0.37234151399380266 1101 18-19 0.4367796328925735 1101 20-21 0.5134168553792122 1101 22-23 0.5206513236639836 1101 24-25 0.421534753611958 1101 26-27 0.5038637354016373 1101 28-29 0.4671017155157742 1101 30-31 0.2981340216019248 1101 32-33 0.40709897666509676 1101 34-35 0.1984876908137707 1101 36-37 0.3273971451999884 1101 38-39 0.3329111198484327 1101 40-41 0.40474810568314723 1101 42-43 0.5317806538828052 1101 44-45 0.44185027932224585 1101 46-47 0.2364010654942561 1101 48-49 0.17291936785577633 1101 50-51 0.035883900842620164 1101 52-53 -0.05748523758413171 1101 54-55 0.08212520862576866 1101 56-57 -3.362729484779983 1101 58-59 -10.083448130309737 1101 60-61 -12.037612973976994 1101 62-63 -4.355837614294128 1101 64-65 -1.3576437932337555 1101 66-67 -2.145549185916714 1101 68-69 -4.760642459693557 1101 70-71 -4.718792638890209 1101 72-73 -3.6723520436655654 1101 74-75 -6.543606488571273 1101 76-77 -4.931232139414039 1101 78-79 1.2462760868700187 1101 80-81 1.823116405535945 1101 82-83 0.3499866101657183 1101 84-85 -0.006349007312170585 1101 86-87 0.08035003486679315 1101 88-89 -0.008615508899605828 1101 90-91 1.5127251373512216 1101 92-93 0.9670362002566009 1101 94-95 -8.380590362561957 1101 96-97 -9.620636408007016 1101 98-99 -8.795406562137718 1101 100-101 -9.652527913887528 1102 1 0.29452341470245713 1102 2 0.3183424033823137 1102 3 0.34282328209319246 1102 4 0.25927295201412903 1102 5 0.3875631194029694 1102 6 0.3609963105138476 1102 7 0.4343175129618899 1102 8 0.34449731374356674 1102 9 0.35755780914992386 1102 10-11 0.3477985355708526 1102 12-13 0.2804470990098338 1102 14-15 0.4524919351878154 1102 16-17 0.4682482561887227 1102 18-19 0.4328103359495614 1102 20-21 0.3850076243260716 1102 22-23 0.3584860836666124 1102 24-25 0.26119042737399667 1102 26-27 0.07537384373118527 1102 28-29 0.28778803863006175 1102 30-31 0.25786621748208916 1102 32-33 0.04928689946849829 1102 34-35 0.18778744458730756 1102 36-37 -0.06883322297243666 1102 38-39 0.11351437016202226 1102 40-41 -0.03726237152670819 1102 42-43 -0.03267983554972176 1102 44-45 -0.12867470314608198 1102 46-47 -0.18768251510729073 1102 48-49 0.052412690287695796 1102 50-51 0.08966601416631903 1102 52-53 0.024957535500199413 1102 54-55 -0.0929384832390241 1102 56-57 -3.4385644412254486 1102 58-59 -9.783219490523642 1102 60-61 -7.9140820085433 1102 62-63 -2.2394618007807523 1102 64-65 -0.6899559603048928 1102 66-67 -2.040545186532185 1102 68-69 -5.032842214360011 1102 70-71 -4.651762087112317 1102 72-73 -3.449592860448462 1102 74-75 -4.233759332223613 1102 76-77 -4.134248365100195 1102 78-79 1.7306527995169887 1102 80-81 2.0685814295885443 1102 82-83 0.7916238071723853 1102 84-85 0.3468368282537888 1102 86-87 0.26225609714693476 1102 88-89 0.21507043672869486 1102 90-91 1.4503368092130131 1102 92-93 0.9922466224935143 1102 94-95 -8.895158190273053 1102 96-97 -9.752962266548247 1102 98-99 -8.801322299550849 1102 100-101 -9.674558175142261 1103 1 0.22829729219857953 1103 2 0.22328866284437154 1103 3 0.23936265294653225 1103 4 0.14682044200988997 1103 5 0.12429723267597126 1103 6 0.19222142178112733 1103 7 0.22454166749184168 1103 8 0.30227449819587093 1103 9 0.3474843176236817 1103 10-11 0.3332385409907843 1103 12-13 0.27532635435969866 1103 14-15 0.3390208136801718 1103 16-17 0.32462940913845273 1103 18-19 0.33115999531657536 1103 20-21 0.34885391586271197 1103 22-23 0.34182586691449046 1103 24-25 0.4407376733729862 1103 26-27 0.4724469575124317 1103 28-29 0.410063980973824 1103 30-31 0.3642896823711794 1103 32-33 0.24637228808710177 1103 34-35 0.19966022477296974 1103 36-37 0.08821625862193372 1103 38-39 0.25059267887443326 1103 40-41 0.29519609839857 1103 42-43 0.36312998247019124 1103 44-45 0.30871868889148146 1103 46-47 0.25765558416289736 1103 48-49 0.30392273277963255 1103 50-51 0.08286329180239704 1103 52-53 0.15040656080061154 1103 54-55 0.1551020427240033 1103 56-57 0.5291465491039347 1103 58-59 -8.626088947503337 1103 60-61 -7.740094001384392 1103 62-63 -2.456415426145213 1103 64-65 -0.6894425921070209 1103 66-67 -1.9124822583659835 1103 68-69 -3.4326602917704854 1103 70-71 -3.9876220119683126 1103 72-73 -3.077974659389625 1103 74-75 -3.9220601497377814 1103 76-77 -0.6872922872682246 1103 78-79 3.19409784637012 1103 80-81 2.705703237724588 1103 82-83 1.1629464496473751 1103 84-85 0.6530831454900863 1103 86-87 0.5493318558931293 1103 88-89 0.42491471879195686 1103 90-91 1.8409716958007643 1103 92-93 1.47733957001196 1103 94-95 -7.362297375678846 1103 96-97 -8.853764368420398 1103 98-99 -8.028713786613645 1103 100-101 -9.655845673082913 1104 1 0.2285410410314732 1104 2 0.17964723404621097 1104 3 0.14046609352532613 1104 4 0.11471758531705234 1104 5 0.1947431819158183 1104 6 0.17112579372686554 1104 7 0.13061804325349868 1104 8 0.1623504685952497 1104 9 0.28018632779059516 1104 10-11 0.29337238060018933 1104 12-13 0.21973682401224437 1104 14-15 0.2478069324136314 1104 16-17 0.1997127221996422 1104 18-19 0.23283397485085544 1104 20-21 0.22230509012090494 1104 22-23 0.26258992166786754 1104 24-25 0.35045687423434657 1104 26-27 0.3426809008298335 1104 28-29 0.31167684211888513 1104 30-31 0.26022692803834957 1104 32-33 0.3025815178335449 1104 34-35 0.22794275938397135 1104 36-37 0.18572020051646376 1104 38-39 0.08792125447534005 1104 40-41 0.1668954336092554 1104 42-43 0.15852995401268544 1104 44-45 0.20484212822857728 1104 46-47 0.13672801960024827 1104 48-49 0.21316663549558967 1104 50-51 0.3486177041031837 1104 52-53 0.28149868119079 1104 54-55 0.13108535426327705 1104 56-57 0.6114855208703744 1104 58-59 -7.958591827677235 1104 60-61 -7.594736655690813 1104 62-63 -2.351101556643364 1104 64-65 -0.4936716947513702 1104 66-67 -1.8996349555349923 1104 68-69 -3.563254828752788 1104 70-71 -4.029520962737088 1104 72-73 -3.1827138227730902 1104 74-75 -3.6866062055845283 1104 76-77 -0.275739051580981 1104 78-79 3.2947644837257286 1104 80-81 2.714678461102636 1104 82-83 1.114963743011053 1104 84-85 0.7552299711869637 1104 86-87 0.7441912316593751 1104 88-89 0.707682026589719 1104 90-91 2.066696824421154 1104 92-93 1.6870244431140016 1104 94-95 -6.425284948836712 1104 96-97 -7.702694748294135 1104 98-99 -5.716838725163091 1104 100-101 -5.105972342756379 1105 1 0.21187290651590374 1105 2 0.25037079832425846 1105 3 0.21914409672049828 1105 4 0.19263869932913735 1105 5 0.26413053019469146 1105 6 0.15679520858547846 1105 7 0.1846023296942647 1105 8 0.2927514422999167 1105 9 0.24929106780614774 1105 10-11 0.162938143545432 1105 12-13 0.30722446567150286 1105 14-15 0.36148141277144674 1105 16-17 0.3884347064093774 1105 18-19 0.38783189381991434 1105 20-21 0.32156221652731887 1105 22-23 0.46809752749267375 1105 24-25 0.3969756101418085 1105 26-27 0.28934489459099666 1105 28-29 0.5160421892367424 1105 30-31 0.39559201283740464 1105 32-33 0.2820905839312502 1105 34-35 0.35398149923096156 1105 36-37 0.4372425610637123 1105 38-39 0.45436061763726343 1105 40-41 0.45305091952879906 1105 42-43 0.4306284192109402 1105 44-45 0.41021656227812997 1105 46-47 0.5885886127816704 1105 48-49 0.4711767106210445 1105 50-51 0.5449933365803332 1105 52-53 0.6488221273780326 1105 54-55 0.43172572702113854 1105 56-57 0.9253455302267284 1105 58-59 -8.219947177797078 1105 60-61 -7.542739055387017 1105 62-63 -2.0475280311449353 1105 64-65 -0.10775527880276314 1105 66-67 -1.4606608919515693 1105 68-69 -3.1195900409327137 1105 70-71 -3.8578053101646113 1105 72-73 -2.728846478907233 1105 74-75 -3.4441054929675623 1105 76-77 0.05621740397113584 1105 78-79 4.090527928511406 1105 80-81 3.525597623349185 1105 82-83 1.9782081673159801 1105 84-85 1.6009214677015322 1105 86-87 1.5041946811042912 1105 88-89 1.4737202290146065 1105 90-91 2.8717354761972054 1105 92-93 2.473808322250374 1105 94-95 -6.210265090261586 1105 96-97 -7.861311329115317 1105 98-99 -5.642108643635922 1105 100-101 -5.022213747554067 1106 1 0.1548449685722737 1106 2 0.2259192611608576 1106 3 0.23044579487559957 1106 4 0.11638430767192887 1106 5 0.21376881105683765 1106 6 0.24500415093815775 1106 7 0.320300964099701 1106 8 0.3038461239041723 1106 9 0.2871073219167002 1106 10-11 0.2335274522483246 1106 12-13 0.3610826562372651 1106 14-15 0.23123774785906193 1106 16-17 0.3195934249745491 1106 18-19 0.3000460966322862 1106 20-21 0.23284597642432203 1106 22-23 0.3651007187397539 1106 24-25 0.2851249727023557 1106 26-27 0.27671896944856655 1106 28-29 0.408431281727637 1106 30-31 0.5129978524669951 1106 32-33 0.4579186263122281 1106 34-35 0.4570535867296357 1106 36-37 0.37482963189147966 1106 38-39 0.5286696730986407 1106 40-41 0.4569970980842726 1106 42-43 0.46127163321316544 1106 44-45 0.373457307518656 1106 46-47 0.30322106192325293 1106 48-49 0.33594094521247797 1106 50-51 0.3225676910174471 1106 52-53 0.397564483964878 1106 54-55 0.29480542100827023 1106 56-57 0.9100389908859583 1106 58-59 -8.139295173333714 1106 60-61 -7.332265637836777 1106 62-63 -1.894830693630297 1106 64-65 -0.1456867141588063 1106 66-67 0.7011642317104503 1106 68-69 -2.412252006965801 1106 70-71 -2.5065932151571175 1106 72-73 -0.9889017301714844 1106 74-75 -0.6268001004365473 1106 76-77 2.7065181497071684 1106 78-79 4.7952588999026275 1106 80-81 3.7664852026420057 1106 82-83 2.0960438223792366 1106 84-85 1.617491189160642 1106 86-87 1.452070879489515 1106 88-89 1.625208759011283 1106 90-91 2.9247376688097866 1106 92-93 2.608973037387024 1106 94-95 -5.932720573365319 1106 96-97 -7.32595354543594 1106 98-99 -4.980326042936305 1106 100-101 -4.4716085040559115 1107 1 0.003353299216556138 1107 2 0.08460915504583966 1107 3 -0.0010201756554870656 1107 4 0.043705036896390936 1107 5 0.004638415638517301 1107 6 0.04734977807537177 1107 7 0.09198072006533664 1107 8 -7.095557217837722E-4 1107 9 0.06240836031636121 1107 10-11 -0.02961736886975075 1107 12-13 -0.029494847657026924 1107 14-15 -0.03708293725720324 1107 16-17 0.07471583681040528 1107 18-19 0.1293379396349863 1107 20-21 0.10010835515582528 1107 22-23 0.009477156608724613 1107 24-25 -0.03792695424520076 1107 26-27 -0.11416264881129479 1107 28-29 -0.12532750218633737 1107 30-31 -0.16165996083464051 1107 32-33 -0.0939350784275419 1107 34-35 -0.15035734374424692 1107 36-37 -0.15081579861605832 1107 38-39 -0.025631339764700556 1107 40-41 -0.08472756698367334 1107 42-43 -0.1125002438689151 1107 44-45 -0.2153875570111623 1107 46-47 -0.06011255385725889 1107 48-49 -0.21307828595854517 1107 50-51 -0.10366507486788379 1107 52-53 -0.05005953549844122 1107 54-55 -0.2008095864552928 1107 56-57 0.35330349609652956 1107 58-59 -8.94293467941484 1107 60-61 -8.024305328243706 1107 62-63 -2.6754977835808234 1107 64-65 -0.7466777555531863 1107 66-67 0.3319805930633173 1107 68-69 -2.858864265792665 1107 70-71 -2.833616272133291 1107 72-73 -1.441048348117949 1107 74-75 -1.423134187478258 1107 76-77 1.6100253892541083 1107 78-79 3.8212282354243428 1107 80-81 2.8801444645494705 1107 82-83 1.1422665362401183 1107 84-85 0.828447721699721 1107 86-87 0.7105300759311426 1107 88-89 0.5082980850613481 1107 90-91 2.080952570878001 1107 92-93 1.6506320044660647 1107 94-95 -6.9727449837664945 1107 96-97 -8.413251721446192 1107 98-99 -6.209331821109252 1107 100-101 -5.45833356377608 1108 1 -0.02249245675040612 1108 2 -0.06871558792630594 1108 3 -0.08780771447147373 1108 4 0.0381888411818565 1108 5 -0.0011546143306162548 1108 6 0.031168178446911554 1108 7 -0.0121258064843488 1108 8 -0.08328675514262329 1108 9 0.01689416800761734 1108 10-11 -0.007444447630916784 1108 12-13 -0.052210282077361114 1108 14-15 -0.16737739237636617 1108 16-17 -0.12375383435786347 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86-87 0.05635528173668192 2311 88-89 0.07906515837991535 2311 90-91 1.5163398269144075 2311 92-93 2.1201559792955216 2311 94-95 -1.2260601885801954 2311 96-97 -6.387215806038604 2311 98-99 -5.771958417686324 2311 100-101 -6.176773960068823 2312 1 -0.2403617341215245 2312 2 -0.25124816455081955 2312 3 -0.2353266451037932 2312 4 -0.2854217134895478 2312 5 -0.26856083548643994 2312 6 -0.2188840592523249 2312 7 -0.26554361289939976 2312 8 -0.2561011275929914 2312 9 -0.3670375378410142 2312 10-11 -0.3099659680135787 2312 12-13 -0.3175552722385149 2312 14-15 -0.3926020587523311 2312 16-17 -0.3341592630943495 2312 18-19 -0.3933831372560874 2312 20-21 -0.43308547647867357 2312 22-23 -0.5004689769406596 2312 24-25 -0.48654826871797496 2312 26-27 -0.38482377190489103 2312 28-29 -0.5054133381564228 2312 30-31 -0.47547500840572354 2312 32-33 -0.6238534028743672 2312 34-35 -0.5145090566316881 2312 36-37 -0.47791215606157067 2312 38-39 -0.4490331375027452 2312 40-41 -0.5490133985743739 2312 42-43 -0.417531063799494 2312 44-45 -0.5851120388750317 2312 46-47 -0.528048056446579 2312 48-49 -0.5475678684935517 2312 50-51 -0.5365290839770083 2312 52-53 -0.6640371677832775 2312 54-55 -0.7739915937469775 2312 56-57 0.07461003123562904 2312 58-59 3.156823906919289 2312 60-61 3.1938009704085317 2312 62-63 0.424096302058075 2312 64-65 -0.3937955719912125 2312 66-67 -2.4513665170891947 2312 68-69 -5.183730968224317 2312 70-71 -4.951857784335356 2312 72-73 -7.479405476116458 2312 74-75 -7.675228902653927 2312 76-77 -5.471643868549414 2312 78-79 -0.7246116213697249 2312 80-81 0.8537099418478284 2312 82-83 -0.09296069916283756 2312 84-85 -0.2569752175253477 2312 86-87 -0.35912378832977865 2312 88-89 -0.20502209186585674 2312 90-91 1.3868710376548812 2312 92-93 2.9893422204792905 2312 94-95 5.067527030860656 2312 96-97 -0.19449782105532876 2312 98-99 -1.2701516422113261 2312 100-101 -0.7857539958025317 2313 1 -0.28789937464096127 2313 2 -0.34145871172954756 2313 3 -0.2181738502764965 2313 4 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64-65 -0.28136404096219536 2313 66-67 -1.9093321547213549 2313 68-69 -4.4016108392537205 2313 70-71 -3.6629065562705314 2313 72-73 -2.917581287663353 2313 74-75 -3.7017258621295497 2313 76-77 0.3871162430933204 2313 78-79 3.084031227549307 2313 80-81 2.436470665433223 2313 82-83 0.9070880880183303 2313 84-85 0.6890815638139749 2313 86-87 0.5320285932785076 2313 88-89 0.6556976031722037 2313 90-91 2.3728228003809484 2313 92-93 3.782728297942608 2313 94-95 5.869846998013426 2313 96-97 1.5290019862668487 2313 98-99 3.074257993467242 2313 100-101 4.380197020395547 2314 1 -0.4304317315829742 2314 2 -0.31063966215239347 2314 3 -0.334171737127523 2314 4 -0.24778584700774076 2314 5 -0.29701718114712605 2314 6 -0.2966117990515542 2314 7 -0.33733452510412576 2314 8 -0.4343215359458199 2314 9 -0.4507091772846863 2314 10-11 -0.4516197616142179 2314 12-13 -0.5846739343755516 2314 14-15 -0.6919630937626735 2314 16-17 -0.677307845280275 2314 18-19 -0.6705138166003692 2314 20-21 -0.5922319856039948 2314 22-23 -0.5922805986408761 2314 24-25 -0.5608957269757227 2314 26-27 -0.6426589435314085 2314 28-29 -0.5263566862865261 2314 30-31 -0.5397560896693818 2314 32-33 -0.5881313703249944 2314 34-35 -0.5270881751903786 2314 36-37 -0.5658559997629311 2314 38-39 -0.6816978806421972 2314 40-41 -0.6023175500260152 2314 42-43 -0.6820496392775297 2314 44-45 -0.5950050978691266 2314 46-47 -0.5503619572996072 2314 48-49 -0.6336498631071876 2314 50-51 -0.7239491209944404 2314 52-53 -0.8591500646163155 2314 54-55 -0.7434118914802781 2314 56-57 0.17283763830197785 2314 58-59 3.3631888574175726 2314 60-61 3.4637435970839903 2314 62-63 0.5827316180810698 2314 64-65 -0.25751067815376416 2314 66-67 -1.8673854490724366 2314 68-69 -4.796529689766572 2314 70-71 -3.623570165119858 2314 72-73 -3.122027929452802 2314 74-75 -4.10976483632021 2314 76-77 0.6848380082670893 2314 78-79 3.3292347557615862 2314 80-81 2.6151648419604037 2314 82-83 1.0709100976513604 2314 84-85 0.6064991222803187 2314 86-87 0.5634986966724647 2314 88-89 0.7737034402927101 2314 90-91 2.360875501490579 2314 92-93 3.9759399997500573 2314 94-95 6.66394081544707 2314 96-97 3.705283525937965 2314 98-99 5.870224326962983 2314 100-101 6.271105444038339 2315 1 -0.4303098919646331 2315 2 -0.4336664439502158 2315 3 -0.4664265642096055 2315 4 -0.2667418744179173 2315 5 -0.4148191432659871 2315 6 -0.5623425143076801 2315 7 -0.42016062862942505 2315 8 -0.5310424725865559 2315 9 -0.6845781394680301 2315 10-11 -0.5220866589198394 2315 12-13 -0.5888020908930898 2315 14-15 -0.8039852956486797 2315 16-17 -0.621218342728092 2315 18-19 -0.639545500763937 2315 20-21 -0.8237627570363841 2315 22-23 -0.8290117258918599 2315 24-25 -0.5704255998009629 2315 26-27 -0.5608210413900423 2315 28-29 -0.5741615744749637 2315 30-31 -0.5673925769575021 2315 32-33 -0.5562511227869678 2315 34-35 -0.5578944112895527 2315 36-37 -0.47754423531099377 2315 38-39 -0.7618071632115146 2315 40-41 -0.669443078410751 2315 42-43 -0.8085485837072639 2315 44-45 -0.7476341100887964 2315 46-47 -0.648550299177522 2315 48-49 -0.5977340912723932 2315 50-51 -0.5626923074895345 2315 52-53 -0.5107002152635971 2315 54-55 -0.4969881187905827 2315 56-57 0.2234042778043488 2315 58-59 3.191290744332708 2315 60-61 3.389735835804366 2315 62-63 0.6231806658293024 2315 64-65 -0.07857187671236687 2315 66-67 -1.7911911836358172 2315 68-69 -4.823829492510043 2315 70-71 -3.3183146456945423 2315 72-73 -2.866461868235593 2315 74-75 -0.6796197640292334 2315 76-77 1.789355188132955 2315 78-79 3.6501168724664588 2315 80-81 2.5334880068910586 2315 82-83 0.8912017772206262 2315 84-85 0.6776087955252237 2315 86-87 0.5460494085494787 2315 88-89 0.605708885233831 2315 90-91 2.2865740672901005 2315 92-93 3.858367539554461 2315 94-95 6.347029625656013 2315 96-97 3.9878779956131716 2315 98-99 6.213702576245986 2315 100-101 6.471582515964753 2316 1 -0.4965138822778421 2316 2 -0.4041801260822595 2316 3 -0.48594461448078263 2316 4 -0.25601049361772965 2316 5 -0.54500861312561 2316 6 -0.6141705202486492 2316 7 -0.3189592957495364 2316 8 -0.5135570479189724 2316 9 -0.5510353834474486 2316 10-11 -0.6102192591656248 2316 12-13 -0.6006338599543 2316 14-15 -0.7501435337730911 2316 16-17 -0.8046333635252836 2316 18-19 -0.6749590033367596 2316 20-21 -0.563811872243555 2316 22-23 -0.8428963959889444 2316 24-25 -0.7208576287121033 2316 26-27 -0.7482274567530993 2316 28-29 -0.8385247718079043 2316 30-31 -0.7316226593190152 2316 32-33 -0.5648104911450744 2316 34-35 -0.4920294802719809 2316 36-37 -0.405293191937254 2316 38-39 -0.7128866001924976 2316 40-41 -0.6785514514073441 2316 42-43 -0.7618510190840553 2316 44-45 -0.6188346157310392 2316 46-47 -0.6752861870711442 2316 48-49 -0.7007522110207134 2316 50-51 -0.5848530846387447 2316 52-53 -0.6277632621258036 2316 54-55 -0.438243865523944 2316 56-57 0.34830688814459876 2316 58-59 3.390550231477821 2316 60-61 3.3966225621006316 2316 62-63 0.6402212204786224 2316 64-65 0.20033713201363668 2316 66-67 -1.7239364933876544 2316 68-69 -2.9427133813271418 2316 70-71 -3.135585367051167 2316 72-73 -0.6279219480721316 2316 74-75 5.61689588941551 2316 76-77 5.875313559156066 2316 78-79 4.923757458799521 2316 80-81 3.3157302426170183 2316 82-83 1.5554015816032525 2316 84-85 1.213184771895424 2316 86-87 1.034745719880636 2316 88-89 1.0597107959905685 2316 90-91 2.40438308785696 2316 92-93 4.160098737064747 2316 94-95 6.784420442453154 2316 96-97 3.876984411354691 2316 98-99 6.109171801113508 2316 100-101 6.12356985976958 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 20.0 4 29.0 5 102.0 6 244.0 7 551.0 8 1368.0 9 2382.0 10 3951.0 11 5992.0 12 8712.0 13 12059.0 14 15008.0 15 19386.0 16 26574.0 17 34617.0 18 38120.0 19 38615.0 20 41196.0 21 42180.0 22 42189.0 23 43212.0 24 45954.0 25 48604.0 26 60484.0 27 85472.0 28 120258.0 29 160073.0 30 193405.0 31 214385.0 32 223710.0 33 224321.0 34 228047.0 35 243335.0 36 245113.0 37 187340.0 38 73522.0 39 4911.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.25317863739198 19.844438650680267 14.211400640865076 23.69098207106267 2 15.319333454961193 21.413145095332233 39.68558589224089 23.581935557465687 3 18.691844343814648 25.987455761214196 30.47620509907185 24.844494795899305 4 12.595098786486295 17.553195183964583 38.086344269396754 31.765361760152373 5 13.042891387154276 38.45333042642642 34.88924794391816 13.61453024250115 6 30.834105602561923 37.64747600481823 17.878407352368626 13.64001104025122 7 27.414552294050875 33.21200024127701 21.910913946359734 17.462533518312377 8 25.517804611538242 35.6775483897855 20.324349657441225 18.480297341235037 9 26.30175346241653 16.4321709995997 20.520939006267717 36.74513653171604 10-11 24.25744257332902 26.755153183485685 28.43983337263224 20.547570870553056 12-13 25.616490187154195 24.161242503504916 28.476865738481305 21.745401570859585 14-15 22.603308866806703 25.484273450906215 26.753412067829725 25.159005614457357 16-17 21.872532964934948 28.58272211335306 27.336123982775014 22.20862093893698 18-19 21.72728746650211 27.703524870920827 29.2146564703547 21.354531192222368 20-21 22.59827925620877 26.922310629531943 28.88425831994429 21.595151794315 22-23 22.234974565381503 26.849031875983613 28.62537905167167 22.29061450696322 24-25 22.312412822573613 26.973031592889118 28.813081910177935 21.90147367435933 26-27 22.302916111682926 27.006747176551638 28.84310589098904 21.847230820776396 28-29 21.95026277538773 27.12177964887242 29.026919488662784 21.901038087077065 30-31 22.173310215799198 27.159659963861333 28.7931385891297 21.873891231209768 32-33 22.06259853854485 27.111226882000523 28.74531470640655 22.08085987304808 34-35 22.469073383231216 26.956739020244484 28.60902837679428 21.96515921973001 36-37 22.46497437189322 26.838373244099188 28.750240803765927 21.94641158024167 38-39 22.365437300418847 26.937217732611746 28.91956531919813 21.777779647771276 40-41 22.310539625472657 26.854895673237966 28.955938401172453 21.878626300116917 42-43 22.09727474189714 27.11639221865 28.561742616687592 22.224590422765267 44-45 22.259000908570975 27.307607443183212 28.301199145365135 22.13219250288068 46-47 22.35145305099729 27.195493024535605 27.915889744925735 22.53716417954137 48-49 22.440039161682428 27.205533709632807 28.00470057264448 22.34972655604028 50-51 22.40914537745663 27.340502182866942 28.06381979672915 22.18653264294728 52-53 22.47375594510422 27.437538454335087 27.866434605499673 22.22227099506102 54-55 22.105961170808282 27.28185782624264 28.078726385540715 22.53345461740836 56-57 22.22704492244659 27.60153277729002 27.929031314018143 22.242390986245244 58-59 21.373565198950242 26.985286754028635 29.427633303584315 22.213514743436807 60-61 21.736506256237806 27.08571520794155 29.002053684840256 22.175724850980387 62-63 22.564907408829118 27.138513496222433 28.0256003053348 22.270978789613643 64-65 22.235561807775433 27.26182137498794 28.080079433088734 22.42253738414789 66-67 21.929934789711233 27.11155453581835 28.658099839302686 22.300410835167728 68-69 21.319766741060565 26.71696802310129 29.985632818565485 21.97763241727266 70-71 22.11668311128268 26.621902881272618 28.972993343626108 22.288420663818595 72-73 21.47108822963401 26.84445306366906 29.171048140313367 22.51341056638356 74-75 21.660236327684963 27.02950921157398 28.827171704212923 22.48308275652813 76-77 20.68888566057934 27.207763262045997 29.228212454928716 22.875138622445945 78-79 21.82173431547797 27.110169608352212 28.312380812337157 22.755715263832663 80-81 21.681169320834012 27.617005590171594 27.833996844694457 22.867828244299933 82-83 22.136842967242586 27.529024559833477 27.413997445385423 22.92013502753851 84-85 22.127973035219455 27.23656696229464 27.388006229390733 23.247453773095174 86-87 22.114960513340286 27.263662676721196 27.651153800105465 22.970223009833052 88-89 22.316980351270214 27.051988635117368 27.579398445005637 23.051632568606777 90-91 21.845048157684325 27.23909391665383 27.97224239490035 22.943615530761498 92-93 21.697570431872425 27.390660290231434 27.795338374740535 23.116430903155603 94-95 20.62653776856462 27.360310418217697 28.502551300466717 23.51060051275097 96-97 20.340598748718534 27.296863731698256 28.56540797094057 23.797129548642644 98-99 21.14086811796916 26.973160935059276 28.00395261279331 23.882018334178255 100-101 20.514024223022975 27.157599637088442 28.471334324919066 23.857041814969513 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 627.0 1 471.5 2 344.5 3 409.0 4 442.5 5 500.5 6 679.5 7 1037.5 8 1484.5 9 1831.0 10 1995.5 11 2021.0 12 2119.0 13 2293.0 14 2520.5 15 2720.5 16 2956.5 17 3247.0 18 3501.5 19 3831.0 20 4353.5 21 5044.0 22 5970.5 23 7288.5 24 8939.0 25 11191.5 26 14368.0 27 18110.0 28 22254.5 29 27210.5 30 32482.5 31 38227.0 32 44890.5 33 51586.0 34 58371.0 35 65226.0 36 71816.5 37 77725.0 38 82092.0 39 85407.0 40 88512.5 41 91427.5 42 94004.0 43 96682.5 44 99893.5 45 103551.0 46 107184.5 47 111929.0 48 117998.5 49 115596.5 50 106535.5 51 100151.5 52 94769.0 53 89663.5 54 84640.0 55 78361.0 56 70877.5 57 62940.0 58 54792.5 59 46542.5 60 38313.0 61 30282.5 62 23400.0 63 17942.5 64 13149.5 65 9366.5 66 6560.0 67 4614.0 68 3080.0 69 1923.0 70 1259.5 71 821.0 72 514.0 73 357.5 74 239.5 75 145.0 76 78.5 77 33.5 78 16.0 79 8.0 80 5.5 81 4.0 82 2.5 83 2.0 84 1.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02573621476578765 2 0.011917622178475532 3 2.1934273948114475E-4 4 0.0 5 0.06309759472407597 6 0.0 7 0.0 8 7.311424649371492E-5 9 0.0 10-11 0.0 12-13 0.0 14-15 2.558998627280022E-4 16-17 1.827856162342873E-5 18-19 4.2040691733886076E-4 20-21 0.0 22-23 0.0 24-25 1.279499313640011E-4 26-27 1.6450705461085858E-4 28-29 0.0010601565741588663 30-31 0.005392175678911476 32-33 0.00575774691138005 34-35 0.0021385917099411614 36-37 0.0881209455865499 38-39 0.0811933707312704 40-41 0.08676833202641618 42-43 0.09046060147434877 44-45 0.09439049222338596 46-47 0.08908970935259163 48-49 0.07899994333645896 50-51 0.07305941080884462 52-53 0.061525638424461106 54-55 0.05001014460170101 56-57 0.06744789239045201 58-59 0.7088060626333192 60-61 0.33362030675082116 62-63 0.003180469722476599 64-65 0.002229984518058305 66-67 1.0870991739918001 68-69 2.3425439005353788 70-71 2.315692693510562 72-73 1.7892518401941913 74-75 1.264017371944967 76-77 0.726956674325384 78-79 0.5559424517765847 80-81 0.03666679461659803 82-83 1.0967136974057237E-4 84-85 9.139280811714365E-4 86-87 0.0010053208892885801 88-89 3.655712324685746E-5 90-91 0.32846575237301423 92-93 0.051911115010537585 94-95 0.9341258917653252 96-97 1.6335002166009551 98-99 0.6303544761455632 100-101 0.5565090871869111 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2735445.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.75661022259129 #Duplication Level Percentage of deduplicated Percentage of total 1 70.23173239901169 33.54029469436938 2 14.851631813458305 14.185271833695298 3 5.781803526613325 8.28358012212229 4 2.9308341938317137 5.598668248874549 5 1.6858704173291126 4.025572820309188 6 1.031565526259577 2.955844365398453 7 0.695533104767819 2.3251412366913837 8 0.4970783303361828 1.8991020857569245 9 0.3485848946770119 1.4982509650115783 >10 1.7792512033785082 14.979847237194582 >50 0.11081127327020497 3.659486203218651 >100 0.05185085474027885 4.422068882967036 >500 0.0019947560106931927 0.6581192882330439 >1k 0.0011508207753825946 0.9985005479747325 >5k 3.0688554010202524E-4 0.970251468182845 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9248 0.3380802757869378 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6800 0.24858843807863074 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5227 0.19108408321132395 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 5206 0.19031638362313993 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 4214 0.15405171736225734 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 2836 0.10367600152808776 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2750 0.10053208892885801 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 3.655712324685746E-5 0.0 18-19 0.0 0.0 0.0 3.655712324685746E-5 0.0 20-21 0.0 0.0 0.0 9.139280811714365E-5 0.0 22-23 0.0 0.0 0.0 2.01064177857716E-4 0.0 24-25 0.0 0.0 0.0 4.5696404058571824E-4 0.0 26-27 0.0 0.0 0.0 8.956495195480078E-4 0.0 28-29 0.0 0.0 0.0 0.0027235056818908808 0.0 30-31 0.0 0.0 0.0 0.0082070741689195 0.0 32-33 0.0 0.0 0.0 0.019941910731160743 0.0 34-35 0.0 0.0 0.0 0.03765383694426318 0.0 36-37 0.0 0.0 0.0 0.06510823650265313 0.0 38-39 0.0 0.0 0.0 0.1056135290601712 0.0 40-41 0.0 0.0 0.0 0.15001215524347958 0.0 42-43 0.0 0.0 0.0 0.19949222155810115 0.0 44-45 0.0 0.0 0.0 0.2580750115611902 0.0 46-47 0.0 0.0 0.0 0.32649166771768395 0.0 48-49 0.0 0.0 0.0 0.39805223647340743 0.0 50-51 0.0 1.827856162342873E-5 0.0 0.4710933687206286 0.0 52-53 0.0 3.655712324685746E-5 0.0 0.5446645792549293 0.0 54-55 0.0 3.655712324685746E-5 0.0 0.625656885808342 0.0 56-57 0.0 3.655712324685746E-5 0.0 0.7030483157219392 0.0 58-59 0.0 7.311424649371492E-5 0.0 0.7632030620246432 0.0 60-61 0.0 7.311424649371492E-5 0.0 0.8152238484049212 0.0 62-63 0.0 7.311424649371492E-5 0.0 0.8615417235586897 0.0 64-65 3.655712324685746E-5 7.311424649371492E-5 0.0 0.9120819464474702 0.0 66-67 3.655712324685746E-5 7.311424649371492E-5 0.0 0.9562795084529209 0.0 68-69 3.655712324685746E-5 7.311424649371492E-5 0.0 1.0080809520937177 0.0 70-71 3.655712324685746E-5 7.311424649371492E-5 0.0 1.0887808016611555 0.0 72-73 3.655712324685746E-5 7.311424649371492E-5 0.0 1.2044292610525893 0.0 74-75 3.655712324685746E-5 7.311424649371492E-5 0.0 1.3582616356753654 0.0 76-77 3.655712324685746E-5 7.311424649371492E-5 0.0 1.5506800538851997 0.0 78-79 3.655712324685746E-5 7.311424649371492E-5 0.0 1.7964170363505754 0.0 80-81 3.655712324685746E-5 7.311424649371492E-5 0.0 2.0911588425283636 3.655712324685746E-5 82-83 3.655712324685746E-5 7.311424649371492E-5 0.0 2.3819524793954914 3.655712324685746E-5 84-85 3.655712324685746E-5 9.139280811714365E-5 0.0 2.649678571493852 3.655712324685746E-5 86-87 7.311424649371492E-5 1.0967136974057237E-4 0.0 2.9006797797067754 3.655712324685746E-5 88-89 7.311424649371492E-5 1.0967136974057237E-4 0.0 3.142486871423114 3.655712324685746E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 10350 0.0 24.805378 1 TACATGG 10355 0.0 24.101377 2 ACATGGG 10270 0.0 23.19454 3 GAGTACT 7555 0.0 21.09311 12-13 CATGGGA 6595 0.0 20.670353 4 GTATCAA 17885 0.0 20.397541 1 AGTACTT 7870 0.0 19.679722 12-13 GTACTTT 8265 0.0 19.480774 14-15 TCAACGC 19705 0.0 18.21684 4 ATCAACG 19710 0.0 18.212217 3 GGTATCA 13785 0.0 18.17921 1 CAACGCA 19910 0.0 18.00558 5 AACGCAG 20050 0.0 17.871033 6 TATCAAC 20230 0.0 17.816967 2 CATGGGG 4345 0.0 17.044125 4 ACGCAGA 21740 0.0 16.373362 7 CGCAGAG 21620 0.0 16.289785 8 ACTTTTT 10030 0.0 16.193722 16-17 AGTACAT 7900 0.0 15.94485 2 GCAGAGT 21970 0.0 15.558166 9 >>END_MODULE