FastQCFastQC Report
Fri 27 May 2016
SRR939643_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939643_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3025457
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT106820.35307062701601777No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70460.2328904360564371No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51080.16883399764068702No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA32980.109008325023294No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG122300.028.2116241
TACATGG123450.027.4495012
GTATCAA175600.027.2701021
ACATGGG126250.025.4878253
TATCAAC205950.023.2518042
CATGGGA89500.023.2262134
TCAACGC210250.022.889134
ATCAACG209900.022.8598423
CAACGCA212100.022.6891085
AACGCAG216100.022.2442266
GGTATCA125750.021.0971581
AGTACTT98750.020.80753312-13
GAGTACT94750.020.10833712-13
ACGCAGA234400.020.1023647
CGCAGAG234300.019.8891498
CATGGGG44850.019.3649314
ATGGGAT40050.018.4859685
ATGGGAG39000.018.3752165
GCAGAGT238850.018.1986879
GTACTTT105950.018.11764714-15
Produced by FastQC (version 0.11.5)