##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939643_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3025457 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.06821382686979 31.0 30.0 33.0 26.0 34.0 2 30.206626304720245 31.0 30.0 34.0 26.0 34.0 3 30.252671249335226 31.0 30.0 34.0 26.0 34.0 4 33.835021618221646 35.0 35.0 37.0 28.0 37.0 5 33.834192983076605 35.0 35.0 37.0 28.0 37.0 6 33.937085868349804 36.0 35.0 37.0 29.0 37.0 7 33.733030084380644 35.0 33.0 37.0 28.0 37.0 8 33.73869203892173 35.0 33.0 37.0 28.0 37.0 9 34.98013391034809 37.0 34.0 39.0 28.0 39.0 10-11 34.86656726570564 37.0 34.0 39.0 27.0 39.0 12-13 34.70333523166913 37.0 34.0 39.0 27.0 39.0 14-15 35.368200076880946 38.0 34.0 40.0 27.0 41.0 16-17 35.097576994153286 38.0 33.0 40.0 26.5 41.0 18-19 35.069613780661896 38.0 33.5 40.0 26.0 41.0 20-21 35.18855234101824 38.0 34.0 40.0 26.0 41.0 22-23 35.03755531808913 38.0 34.0 40.0 25.0 41.0 24-25 34.899726057914556 38.0 33.0 40.0 25.0 41.0 26-27 35.02858411142515 38.0 34.0 40.0 25.0 41.0 28-29 34.89983794183821 38.0 34.0 40.0 25.0 41.0 30-31 34.66593228725445 38.0 33.0 40.0 24.0 41.0 32-33 34.42794592684675 38.0 33.0 40.0 24.0 41.0 34-35 34.18420787338904 38.0 33.0 40.0 23.5 41.0 36-37 33.88703293419804 38.0 33.0 40.0 22.5 41.0 38-39 33.72015450888907 38.0 32.5 40.0 21.0 41.0 40-41 33.42195955850637 37.5 31.5 40.0 18.5 41.0 42-43 33.097938592417606 37.0 31.0 40.0 17.0 41.0 44-45 32.83286789400742 37.0 31.0 40.0 15.5 41.0 46-47 32.3586109470404 37.0 30.5 40.0 13.5 41.0 48-49 31.99067678040045 37.0 30.0 39.5 9.0 41.0 50-51 31.379889550570375 35.5 29.5 38.5 9.0 40.0 52-53 31.428792575799292 36.0 30.0 39.0 8.0 40.0 54-55 31.46300938998637 36.0 30.0 39.0 7.5 40.0 56-57 31.319143025334686 36.0 29.5 39.0 6.5 40.0 58-59 30.90886071096036 36.0 28.5 39.0 2.0 40.0 60-61 30.479984676695125 35.0 28.0 39.0 2.0 40.0 62-63 30.058542230149033 35.0 28.0 38.0 2.0 40.0 64-65 29.427374112406817 34.0 26.5 38.0 2.0 40.0 66-67 28.878155267121628 34.0 26.0 38.0 2.0 39.5 68-69 28.357205539526756 34.0 26.0 37.0 2.0 39.0 70-71 27.78822224212739 33.0 25.5 36.0 2.0 39.0 72-73 27.031073156881753 32.0 23.5 36.0 2.0 38.5 74-75 26.560455494822765 32.0 22.0 35.0 2.0 38.0 76-77 26.158620829844878 32.0 21.5 35.0 2.0 37.5 78-79 25.561828345271476 31.0 20.0 35.0 2.0 37.0 80-81 24.87932302458769 31.0 18.0 34.0 2.0 36.0 82-83 24.195998488823342 30.5 13.0 34.0 2.0 36.0 84-85 23.512214683599865 30.0 7.0 34.0 2.0 35.0 86-87 22.80895613456083 29.5 2.0 34.0 2.0 35.0 88-89 21.984689585738614 29.0 2.0 34.0 2.0 35.0 90-91 20.72589099762449 27.5 2.0 33.5 2.0 35.0 92-93 19.081432325761035 25.0 2.0 32.0 2.0 34.0 94-95 18.77534286555717 25.0 2.0 32.0 2.0 34.0 96-97 18.11730938499539 24.5 2.0 32.0 2.0 34.0 98-99 17.10663612141901 21.0 2.0 32.0 2.0 34.0 100-101 15.525911787872047 7.5 2.0 30.5 2.0 34.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.9574488771609353 1101 2 0.9480718781595279 1101 3 0.8326915795229475 1101 4 1.0454962643747834 1101 5 0.826810807569224 1101 6 0.7425570928326763 1101 7 0.810232202222636 1101 8 1.0551292946583217 1101 9 1.1821483737161245 1101 10-11 0.9321040984233946 1101 12-13 0.9077652981969564 1101 14-15 1.2115389377401655 1101 16-17 0.9078411032636566 1101 18-19 0.9156917772528814 1101 20-21 1.1322406923063326 1101 22-23 1.0758672887639875 1101 24-25 1.0487267803300142 1101 26-27 1.0019102514041194 1101 28-29 0.9945702357821204 1101 30-31 1.0787727731742578 1101 32-33 0.9364094604145592 1101 34-35 1.0977151409835386 1101 36-37 1.090580340716393 1101 38-39 0.9334897756572076 1101 40-41 1.0031986767605332 1101 42-43 0.9679334561624202 1101 44-45 0.9899138754305028 1101 46-47 0.8076729629069419 1101 48-49 0.8925874147634829 1101 50-51 0.9447532473419074 1101 52-53 0.9720921256952586 1101 54-55 0.9827671648630592 1101 56-57 0.8844142858064608 1101 58-59 0.7535871447482947 1101 60-61 0.5135796020464802 1101 62-63 0.6314270089696841 1101 64-65 0.5034372081478722 1101 66-67 0.3137116517380427 1101 68-69 0.39897590753319534 1101 70-71 0.223958713790946 1101 72-73 0.4137664122407436 1101 74-75 0.12530345875412863 1101 76-77 -0.5619465705979145 1101 78-79 -0.2194613165054733 1101 80-81 -0.2079200860254602 1101 82-83 -0.3434017832335279 1101 84-85 -0.6992405023912767 1101 86-87 -1.177927965694149 1101 88-89 -1.762768288801201 1101 90-91 -4.814772515104224 1101 92-93 -9.549476153205106 1101 94-95 -7.866789634173532 1101 96-97 -4.54817157661282 1101 98-99 -3.5566586129737807 1101 100-101 -2.997206261705321 1102 1 0.9018193555937657 1102 2 0.8214908187786953 1102 3 0.8029644193757193 1102 4 0.8821612259000133 1102 5 0.8438227510228344 1102 6 0.8772592471095066 1102 7 0.8818863214596746 1102 8 0.8249247236162205 1102 9 0.9401236417017813 1102 10-11 0.8119979835106435 1102 12-13 0.815338881939212 1102 14-15 0.8881076965636936 1102 16-17 1.022767635781051 1102 18-19 0.9038951552701064 1102 20-21 1.1101611919529617 1102 22-23 0.9990852580840794 1102 24-25 0.95376439678342 1102 26-27 0.8297380447501652 1102 28-29 0.9453939138238567 1102 30-31 1.0191982461500615 1102 32-33 0.8780256617842923 1102 34-35 0.9041129809080743 1102 36-37 0.9294125398723239 1102 38-39 0.893535642239371 1102 40-41 1.01811658309947 1102 42-43 0.8159984651101055 1102 44-45 0.703836139927219 1102 46-47 0.556794843775549 1102 48-49 0.6696574679443756 1102 50-51 0.684373127109339 1102 52-53 0.826364251340646 1102 54-55 0.87118374297145 1102 56-57 0.8520153068244802 1102 58-59 0.7962809868912402 1102 60-61 0.6645748994156797 1102 62-63 0.5855502361536544 1102 64-65 0.5879146224874816 1102 66-67 0.5632616120949372 1102 68-69 0.37610447806777714 1102 70-71 0.5086126274924148 1102 72-73 0.38720259086378306 1102 74-75 0.3558134625375935 1102 76-77 0.16196081840570287 1102 78-79 -0.2028882173219273 1102 80-81 -0.43060265815954324 1102 82-83 -0.6171893673925908 1102 84-85 -0.776874960256773 1102 86-87 -1.3643256161585242 1102 88-89 -2.022958374752964 1102 90-91 -5.668022130012098 1102 92-93 -10.283163561740341 1102 94-95 -8.862660335652642 1102 96-97 -5.716813221907806 1102 98-99 -4.3973301545077526 1102 100-101 -3.815500405491804 1103 1 0.8759529187839661 1103 2 0.8114588482708669 1103 3 0.7604228753270661 1103 4 0.814158957960089 1103 5 0.8048670070691486 1103 6 0.6224208790602717 1103 7 0.7003484032640728 1103 8 0.5971372997507132 1103 9 0.7216396956936393 1103 10-11 0.6271151719908019 1103 12-13 0.5805913728504208 1103 14-15 0.6943429056831363 1103 16-17 0.8462508861635314 1103 18-19 0.6087622141966094 1103 20-21 0.7946419424417499 1103 22-23 0.8287937396869864 1103 24-25 0.7628478425300926 1103 26-27 0.6194498766653354 1103 28-29 0.8171644230889896 1103 30-31 0.9068317889014708 1103 32-33 0.9219473247925976 1103 34-35 0.9633568446474357 1103 36-37 0.7892717113108816 1103 38-39 0.7834941414832457 1103 40-41 0.5699701457100801 1103 42-43 0.6750035071886842 1103 44-45 0.512358196326268 1103 46-47 0.6333394655407218 1103 48-49 0.48003497768932135 1103 50-51 0.4114922555663654 1103 52-53 0.5033393128366619 1103 54-55 0.47029626363259425 1103 56-57 0.23831744208309047 1103 58-59 0.349202373180713 1103 60-61 0.1868017002409097 1103 62-63 0.4549814758834607 1103 64-65 0.32829805418028 1103 66-67 0.2346079369590761 1103 68-69 0.1333664624728037 1103 70-71 0.36887132720135796 1103 72-73 0.39973021415297794 1103 74-75 0.25121186048989586 1103 76-77 -0.17003398036410644 1103 78-79 -0.32556577886477456 1103 80-81 -0.23693935441003333 1103 82-83 -0.31405979406616424 1103 84-85 -0.5054086060974328 1103 86-87 -0.7626369833886812 1103 88-89 -1.0787502035015883 1103 90-91 -2.5907830017105695 1103 92-93 -9.202822626237506 1103 94-95 -5.994454539777367 1103 96-97 -3.417434490429798 1103 98-99 -2.6455603732640576 1103 100-101 -2.2073805632674794 1104 1 0.9062656156623561 1104 2 0.805180720736157 1104 3 0.7928399462260849 1104 4 0.830777858919781 1104 5 0.9762225211732058 1104 6 0.8272163978360965 1104 7 1.0409107675030782 1104 8 0.9063864517075544 1104 9 1.0982775899329624 1104 10-11 0.9166698414800649 1104 12-13 0.9453552959425195 1104 14-15 1.0609772862532125 1104 16-17 1.0811145775590418 1104 18-19 0.8062990478502314 1104 20-21 0.9664118682981879 1104 22-23 0.931003895601819 1104 24-25 0.7699562152222512 1104 26-27 0.8226441224277394 1104 28-29 0.8286689285796882 1104 30-31 0.8484107222835888 1104 32-33 0.9231137908057434 1104 34-35 0.835117321981528 1104 36-37 0.6567727532182559 1104 38-39 0.6422684231741016 1104 40-41 0.705884405619031 1104 42-43 0.8477048889366543 1104 44-45 0.819616542438979 1104 46-47 0.6385095788584678 1104 48-49 0.419163364626435 1104 50-51 0.7156316050859104 1104 52-53 0.7354933889281092 1104 54-55 0.6993077765039679 1104 56-57 0.7659382396367924 1104 58-59 0.46893111747755967 1104 60-61 0.43860919490944283 1104 62-63 0.4818993755513077 1104 64-65 0.6877755618306836 1104 66-67 0.5921886865571544 1104 68-69 0.506962676152451 1104 70-71 0.7671498370512815 1104 72-73 0.636698935716506 1104 74-75 0.47660991503477135 1104 76-77 0.3256723005083124 1104 78-79 0.164933917831096 1104 80-81 0.14833721225940621 1104 82-83 0.14824519483017795 1104 84-85 -0.1324586975917157 1104 86-87 -0.23844357169895503 1104 88-89 -0.34642175624265903 1104 90-91 -0.6105945518073703 1104 92-93 -4.077890031711547 1104 94-95 -1.6882925065865884 1104 96-97 -1.0297331311001976 1104 98-99 -0.8328336401683032 1104 100-101 -0.8415388087381217 1105 1 0.9376350759303662 1105 2 0.9476944698780194 1105 3 0.8705344387873204 1105 4 0.8212019617707966 1105 5 0.6703279322362903 1105 6 0.75358286343468 1105 7 0.8127466548497537 1105 8 0.8192913200667462 1105 9 1.1499968206530014 1105 10-11 0.934411550754568 1105 12-13 0.9609344593729929 1105 14-15 1.1451576821912184 1105 16-17 1.1190508503867633 1105 18-19 0.940605798678348 1105 20-21 0.8479012128893757 1105 22-23 0.8354648908725579 1105 24-25 0.9784499127599844 1105 26-27 1.0824590078531813 1105 28-29 1.00979597561453 1105 30-31 1.1104575739571771 1105 32-33 1.2338853121606803 1105 34-35 1.0722757394521096 1105 36-37 1.1181465033529179 1105 38-39 1.188055000248525 1105 40-41 1.1962595727753396 1105 42-43 1.2219420502805178 1105 44-45 1.0593507224447336 1105 46-47 1.0515943658294162 1105 48-49 0.9218659372674907 1105 50-51 0.7546954582012262 1105 52-53 0.750993106369183 1105 54-55 0.9364586612374843 1105 56-57 0.8380921602259228 1105 58-59 0.7155947110765339 1105 60-61 0.4670389909922896 1105 62-63 0.6806896071412432 1105 64-65 0.8376248962510431 1105 66-67 0.8738812958257398 1105 68-69 0.7587771445135445 1105 70-71 0.6647092194058608 1105 72-73 0.7736569932197952 1105 74-75 0.6379684046028515 1105 76-77 0.4590564807199975 1105 78-79 0.2932684004035977 1105 80-81 0.25987520857736257 1105 82-83 0.47554988179672364 1105 84-85 0.31473562339652617 1105 86-87 0.06019395667887295 1105 88-89 -0.22021757506418638 1105 90-91 -1.3913391200536438 1105 92-93 -7.193571195988728 1105 94-95 -3.1043216724774005 1105 96-97 -1.4179531659263702 1105 98-99 -0.8492901236005039 1105 100-101 -0.6004341041265473 1106 1 0.7571497125181743 1106 2 0.7686210875526065 1106 3 0.891457801174095 1106 4 0.8798581789636017 1106 5 0.665306650792246 1106 6 0.6831302970471569 1106 7 0.6802645792782442 1106 8 0.7872267351566293 1106 9 0.8829818381908652 1106 10-11 0.7326976417235826 1106 12-13 0.7773423306589109 1106 14-15 0.8036840947826747 1106 16-17 0.9117948138624286 1106 18-19 0.7725230818151374 1106 20-21 0.7870802293041592 1106 22-23 0.9331214905616818 1106 24-25 0.7542297576455184 1106 26-27 0.697006482156759 1106 28-29 0.9096897718497914 1106 30-31 0.8977794486774258 1106 32-33 0.7679946247252758 1106 34-35 0.8098873347872839 1106 36-37 0.9669424537748839 1106 38-39 0.6373742381856431 1106 40-41 0.7444764888784334 1106 42-43 0.8267155229401695 1106 44-45 0.7155150168204116 1106 46-47 0.7665979873371 1106 48-49 0.8533014378086747 1106 50-51 0.5701226740349412 1106 52-53 0.6626181460429983 1106 54-55 0.7416927571219851 1106 56-57 0.789712479732426 1106 58-59 0.7657566616449891 1106 60-61 0.7046973810897015 1106 62-63 0.7127826758196782 1106 64-65 0.796445505478161 1106 66-67 0.6487374527957179 1106 68-69 0.5024211950681625 1106 70-71 0.5150209132134904 1106 72-73 0.5082777928812447 1106 74-75 0.5880648425041706 1106 76-77 0.48872842909113245 1106 78-79 0.18603311633903985 1106 80-81 0.3535603571405588 1106 82-83 0.28988047255007743 1106 84-85 0.10128233270746279 1106 86-87 -0.02854722352572381 1106 88-89 -0.089104754926975 1106 90-91 -0.41176605104967834 1106 92-93 -3.679752499243202 1106 94-95 -1.5688268280394695 1106 96-97 -0.7933692323993462 1106 98-99 -0.45533112395045094 1106 100-101 -0.25299723636447524 1107 1 0.5627459178605712 1107 2 0.5170677152293521 1107 3 0.5233292030328087 1107 4 0.5882521694007394 1107 5 0.5352936005169298 1107 6 0.5290773439248255 1107 7 0.3708029391270955 1107 8 0.40626751874901856 1107 9 0.46866862480827365 1107 10-11 0.41986752099371927 1107 12-13 0.37679759175757965 1107 14-15 0.39220604524413005 1107 16-17 0.5613334705394095 1107 18-19 0.5217621686176841 1107 20-21 0.5424546286612184 1107 22-23 0.5854660411190054 1107 24-25 0.6843300144166733 1107 26-27 0.5524801347433552 1107 28-29 0.5987069933860454 1107 30-31 0.5103003875519931 1107 32-33 0.3053532608571743 1107 34-35 0.37841006648683617 1107 36-37 0.47060146388482593 1107 38-39 0.4272471800784672 1107 40-41 0.38637721998178876 1107 42-43 0.10453294484599951 1107 44-45 0.05630351047481241 1107 46-47 0.34227063545052516 1107 48-49 0.3596022606281153 1107 50-51 0.3716690385043151 1107 52-53 0.5374603105425244 1107 54-55 0.5893661496346319 1107 56-57 0.42377161289265786 1107 58-59 0.4140052168838153 1107 60-61 0.2924699569650677 1107 62-63 0.3589669311409871 1107 64-65 0.4570893875158646 1107 66-67 0.31142605086925457 1107 68-69 0.29561995643528505 1107 70-71 0.20860816586376885 1107 72-73 0.35012746827575825 1107 74-75 0.34719802390891985 1107 76-77 -1.4459156065171896E-4 1107 78-79 0.04672248591166195 1107 80-81 -0.04596559647803389 1107 82-83 -0.03729272433546882 1107 84-85 -0.0911207831833849 1107 86-87 -0.058868471169304826 1107 88-89 -0.24436804486665764 1107 90-91 -0.6508877340694141 1107 92-93 -3.939750600135973 1107 94-95 -1.8651051283363813 1107 96-97 -1.1305347079425694 1107 98-99 -1.066625529608224 1107 100-101 -0.9479925701076954 1108 1 0.3676576238493894 1108 2 0.4893277557999127 1108 3 0.07145952550767731 1108 4 -0.3556315856585286 1108 5 -1.0497048563873008 1108 6 -0.08967110896092123 1108 7 -0.007888636212946665 1108 8 0.19281799244727438 1108 9 0.30115513075723044 1108 10-11 0.37014778978171137 1108 12-13 0.3159442638143588 1108 14-15 0.32637181311108776 1108 16-17 0.40197578013585655 1108 18-19 0.27323373547069707 1108 20-21 0.20673917471780356 1108 22-23 0.1264596461122096 1108 24-25 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72-73 0.6942414841191962 2311 74-75 0.9017981056045166 2311 76-77 0.7919400192225545 2311 78-79 1.035677516616353 2311 80-81 0.9988263283558965 2311 82-83 1.0781405530042498 2311 84-85 1.0231145024299302 2311 86-87 1.2309935106985535 2311 88-89 1.2448223005190684 2311 90-91 2.154551773706963 2311 92-93 3.164705883189864 2311 94-95 2.3946490046346796 2311 96-97 2.2310997815211415 2311 98-99 1.9540355965977056 2311 100-101 1.9565222590199998 2312 1 0.30778420634473136 2312 2 0.07064508329466435 2312 3 0.0637671709586698 2312 4 -0.007247140964082632 2312 5 0.1825034388035789 2312 6 0.13811789660944385 2312 7 0.2828909507654487 2312 8 -0.5517467438829726 2312 9 -0.004046157643863069 2312 10-11 0.0888581304359235 2312 12-13 0.26342760439072066 2312 14-15 -0.122386099246647 2312 16-17 0.1994445014219437 2312 18-19 0.21897494395681605 2312 20-21 0.4478425846862919 2312 22-23 0.4278473555911262 2312 24-25 0.49068337387070926 2312 26-27 0.5168082496591566 2312 28-29 0.40822211844344736 2312 30-31 0.482171625645492 2312 32-33 0.4855721361110241 2312 34-35 0.3829059500361325 2312 36-37 0.5995106998087181 2312 38-39 0.6431223969244826 2312 40-41 0.7447954131528718 2312 42-43 0.8277569912193812 2312 44-45 0.6650327642041063 2312 46-47 0.7168906304460947 2312 48-49 0.644824099634917 2312 50-51 0.4760273764530183 2312 52-53 0.5096581526862529 2312 54-55 0.6540926833481784 2312 56-57 0.508834517309527 2312 58-59 0.610181489237835 2312 60-61 0.6213471237087838 2312 62-63 0.3581476079294248 2312 64-65 0.6331633474559943 2312 66-67 0.5994480070530095 2312 68-69 0.3710204826635426 2312 70-71 0.3728298793783331 2312 72-73 0.5600407069826012 2312 74-75 0.8025949722732513 2312 76-77 0.876569531389702 2312 78-79 0.9726366721040023 2312 80-81 0.9499303971535227 2312 82-83 1.0020546244597277 2312 84-85 0.7673332649140896 2312 86-87 0.8207281113222002 2312 88-89 1.212816643477744 2312 90-91 1.5771034917723732 2312 92-93 2.5092250265242235 2312 94-95 1.859047093372176 2312 96-97 1.7952632834949647 2312 98-99 1.9760969978894565 2312 100-101 2.093136844816195 2313 1 -0.2664500102072651 2313 2 -0.9440648674205576 2313 3 -0.4020920042840608 2313 4 -0.23435401333119898 2313 5 -0.9038655764043 2313 6 -0.5465058308906521 2313 7 -1.2378705189392605 2313 8 -1.029525098578425 2313 9 -1.3105624245926748 2313 10-11 -1.9807686131957922 2313 12-13 -0.7082362536534674 2313 14-15 -0.3347682959056755 2313 16-17 -1.667161742935555 2313 18-19 -1.2992965521087996 2313 20-21 -1.5487297541291767 2313 22-23 -1.2838919252257313 2313 24-25 -0.4004984304963983 2313 26-27 -1.0364293956867172 2313 28-29 -2.4221339119485705 2313 30-31 -0.7336488341929268 2313 32-33 -0.44380983373781646 2313 34-35 -0.11958203006545887 2313 36-37 -0.24671130650735762 2313 38-39 -0.5978559171027911 2313 40-41 -0.331857054996064 2313 42-43 -0.085053245124449 2313 44-45 -0.6595413278369833 2313 46-47 -0.8294731616935493 2313 48-49 -0.3543234818552321 2313 50-51 -0.5620911899144261 2313 52-53 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2315 30-31 -2.5171436044570186 2315 32-33 -1.7290186487107633 2315 34-35 -2.4100032875423665 2315 36-37 -2.6322420966291773 2315 38-39 -2.059224799876805 2315 40-41 -3.482441486007019 2315 42-43 -7.531524831235146 2315 44-45 -4.206622567918803 2315 46-47 -2.47936749033569 2315 48-49 -5.380139320022323 2315 50-51 -6.351754487679621 2315 52-53 -6.604026547430962 2315 54-55 -4.223496008879788 2315 56-57 -3.431622676562082 2315 58-59 -3.753921553155177 2315 60-61 -2.8145495080758884 2315 62-63 -2.9354997529505518 2315 64-65 -1.825538266912119 2315 66-67 -2.4202217503098247 2315 68-69 -1.7445290071665909 2315 70-71 -1.6314670381525112 2315 72-73 -1.852229055430481 2315 74-75 -2.5230788214850257 2315 76-77 -2.4623445951526257 2315 78-79 -1.4391530454324837 2315 80-81 -1.2079564442136395 2315 82-83 -1.5943614929927001 2315 84-85 -1.4003005509892397 2315 86-87 -1.9417409050120895 2315 88-89 -1.2863914725388916 2315 90-91 -0.4526117748222305 2315 92-93 0.35181972338593326 2315 94-95 0.33859685580697985 2315 96-97 0.14066236016882883 2315 98-99 -0.03438171926120859 2315 100-101 0.007914081457411015 2316 1 -4.335214712976022 2316 2 -2.894033730114934 2316 3 -4.692423790341085 2316 4 -4.426737271448644 2316 5 -4.183092690024399 2316 6 -4.530054287028015 2316 7 -3.046789090167284 2316 8 -1.7115977891131848 2316 9 -1.9666462122514332 2316 10-11 -3.185874671047202 2316 12-13 -4.0513958731937905 2316 14-15 -3.171318337675231 2316 16-17 -2.961768061990526 2316 18-19 -3.0719728645128654 2316 20-21 -5.564761485183862 2316 22-23 -6.4908286173070024 2316 24-25 -4.325762499242408 2316 26-27 -3.9446464685038265 2316 28-29 -4.436881073805026 2316 30-31 -6.185143678011606 2316 32-33 -4.5447643428937425 2316 34-35 -6.063968454844591 2316 36-37 -9.379260492173607 2316 38-39 -6.06154641274518 2316 40-41 -5.471489298792186 2316 42-43 -4.648431124980021 2316 44-45 -3.488702737829918 2316 46-47 -5.634621196223048 2316 48-49 -3.093648310922241 2316 50-51 -4.245922022060647 2316 52-53 -5.407717838796803 2316 54-55 -7.143950392388849 2316 56-57 -5.036683738368627 2316 58-59 -6.063949940631588 2316 60-61 -6.41798423182092 2316 62-63 -6.388343488512593 2316 64-65 -5.172134321167825 2316 66-67 -6.433366737908983 2316 68-69 -5.465237659715374 2316 70-71 -6.24941150903064 2316 72-73 -8.221673787197563 2316 74-75 -8.064390207966152 2316 76-77 -7.370899086465137 2316 78-79 -6.175189785952561 2316 80-81 -6.219015883221942 2316 82-83 -5.1958737294891115 2316 84-85 -6.503812736916867 2316 86-87 -5.361322747140942 2316 88-89 -5.335643813069087 2316 90-91 -5.726590595696106 2316 92-93 -4.310145793300617 2316 94-95 -4.3439347094577805 2316 96-97 -4.918189268720656 2316 98-99 -5.873144255147805 2316 100-101 -4.977056002509757 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 17216.0 3 7016.0 4 7397.0 5 8278.0 6 9366.0 7 11172.0 8 13465.0 9 16432.0 10 19600.0 11 23311.0 12 27336.0 13 30714.0 14 33536.0 15 37043.0 16 41510.0 17 45638.0 18 42426.0 19 40904.0 20 43552.0 21 47061.0 22 51532.0 23 57454.0 24 64880.0 25 72356.0 26 81539.0 27 92474.0 28 103622.0 29 117820.0 30 134713.0 31 153138.0 32 175851.0 33 201970.0 34 237225.0 35 281776.0 36 319462.0 37 273045.0 38 82286.0 39 1341.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.528424851834146 18.866810352670292 13.301365127307674 23.303399668187886 2 15.132193206218755 20.6535383312324 40.774234318289345 23.440034144259496 3 19.10167116174061 25.829460247048203 29.84098289930946 25.22788569190172 4 12.219360695906731 16.64867919459357 38.35176084227689 32.78019926722281 5 13.200917882834956 38.29302377284782 34.41048260310301 14.095575741214208 6 30.951574456993114 36.83869465319076 18.29134847509522 13.91838241472091 7 27.72241065130885 33.28011296040695 21.625082874244207 17.372393514039988 8 25.867050684536334 35.04285464751606 20.466650522566013 18.623444145381598 9 27.174202136457527 16.199881704878813 20.321586742477873 36.30432941618579 10-11 24.57442697836538 26.675230617967493 28.20130887523538 20.549033528431746 12-13 26.141780630110034 24.118632805890055 28.219613042853297 21.51997352114661 14-15 22.915457373694398 25.143919993369945 26.787439090094473 25.153183542841184 16-17 22.181289151863325 28.49959555825367 27.186954644628862 22.132160645254135 18-19 21.967214524901422 27.303137691419128 29.440411753038827 21.289236030640623 20-21 23.020950500952836 26.398835585129888 29.156743866944623 21.42347004697265 22-23 22.532447742613947 26.584581204647368 28.89598754207874 21.986983510659947 24-25 22.3818204234763 26.618387268404938 29.18052727259187 21.819265035526897 26-27 22.319852479967057 26.725709324498727 29.11614091195105 21.838297283583167 28-29 22.007154898454132 26.939288290175885 29.260889986485267 21.79266682488472 30-31 22.19937377414858 26.788127361607778 29.209866094118574 21.802632770125065 32-33 22.022036451150637 26.83454357782109 29.059570772947303 22.083849198080973 34-35 22.535274898709247 26.76126262317854 28.870053103279254 21.83340937483296 36-37 22.530385591928642 26.641182741850976 28.8625142366523 21.965917429568083 38-39 22.3876563701037 26.809019759372692 29.05573514944261 21.747588721080994 40-41 22.46547347588521 26.689673317690353 29.04548598585513 21.7993672205693 42-43 22.179886272098408 26.84615888678772 28.774989260649498 22.198965580464378 44-45 22.318356047065958 27.050118022282106 28.481574592299445 22.149951338352494 46-47 22.45287505872879 27.06108472717157 28.04438547798988 22.44165473610976 48-49 22.620407837630314 27.107154745015826 27.968834596167124 22.30360282118674 50-51 22.478470508934258 27.34884330715231 27.94359425633039 22.229091927583042 52-53 22.60324057082228 27.368193813768745 27.834994542651337 22.19357107275764 54-55 22.20870101865374 27.125748958981365 28.08376421478374 22.581785807581152 56-57 22.461139746312984 27.626762830193208 27.647946167843195 22.264151255650614 58-59 22.58864222471696 27.19551927007763 27.75064604272355 22.465192462481866 60-61 22.46005197607545 27.15492626794072 28.089022887841057 22.295998868142767 62-63 22.552428286205874 27.134684824663214 28.004278387754706 22.308608501376213 64-65 22.358844106066304 27.158358752779705 27.98051108680521 22.50228605434878 66-67 22.191027504044907 27.41624667695447 27.74554795461232 22.647177864388304 68-69 22.21029402007126 27.353547995744897 27.910437660408167 22.525720323775676 70-71 22.163745486756767 27.29243135377522 27.798467296889367 22.74535586257864 72-73 22.277680045181373 27.1700167576275 27.689430379704934 22.862872817486195 74-75 22.212799024210646 27.400851696705388 27.54020760686602 22.84614167221795 76-77 22.338923737283515 27.120978111816136 27.65823699553548 22.881861155364867 78-79 22.172590593862797 27.237665472560813 27.66850707631467 22.92123685726172 80-81 22.07342183873963 27.44631158214216 27.625034291747312 22.855232287370885 82-83 22.170628572242173 27.390069350938195 27.460001400627696 22.979300676191926 84-85 22.15938499073497 27.153811076158625 27.366422606175416 23.320381326930985 86-87 22.233198277097788 27.021035072136847 27.66265426387179 23.083112386893575 88-89 22.23619685681643 26.925824490104727 27.56052711872853 23.277451534350313 90-91 21.817587405710267 27.036768418302003 27.876687099181126 23.2689570768066 92-93 21.55835093714877 27.02135135831123 28.228999402730164 23.191298301809834 94-95 21.905888468746483 27.009790936843984 27.481590257405287 23.602730337004243 96-97 21.9492850881681 26.84584416620885 27.404269171250668 23.800601574372386 98-99 21.87025120661582 26.796741375495664 27.2582230718988 24.074784345989716 100-101 21.929610563102578 26.753670094832277 27.474497438937195 23.842221903127946 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2995.0 1 1816.5 2 702.5 3 749.5 4 664.0 5 664.0 6 968.0 7 1705.0 8 2591.5 9 2991.0 10 2705.0 11 2400.5 12 2534.0 13 2716.0 14 2926.0 15 3235.0 16 3489.0 17 3754.5 18 4078.5 19 4511.5 20 5126.5 21 5963.5 22 7089.0 23 8597.0 24 10715.5 25 13526.5 26 17220.5 27 21791.0 28 26799.0 29 31848.0 30 37053.5 31 42534.5 32 48026.0 33 53521.5 34 58854.5 35 64031.0 36 70350.5 37 77000.5 38 81690.5 39 84248.5 40 86978.0 41 91832.0 42 96119.5 43 99721.5 44 103804.0 45 107553.5 46 110941.0 47 116075.5 48 128022.0 49 130004.0 50 120409.0 51 114299.5 52 109084.5 53 105080.5 54 100209.0 55 92751.5 56 84427.5 57 75505.0 58 66801.5 59 58564.0 60 50108.5 61 41136.0 62 32583.5 63 25047.0 64 18620.0 65 13784.0 66 9810.5 67 7089.0 68 5033.5 69 3303.5 70 2277.0 71 1627.0 72 1153.5 73 851.5 74 642.0 75 492.5 76 343.0 77 204.5 78 125.5 79 82.5 80 64.0 81 45.5 82 33.0 83 28.0 84 16.5 85 14.5 86 17.0 87 10.5 88 9.0 89 7.0 90 3.5 91 5.5 92 5.0 93 3.5 94 2.5 95 2.0 96 2.5 97 1.5 98 0.5 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08798670746270729 2 0.0987288862475983 3 0.09548970618323116 4 0.09859667481639965 5 0.09416759187124457 6 0.07876496013660085 7 0.09271326612805933 8 0.08861471176090091 9 0.0829296202193586 10-11 0.08954019177929153 12-13 0.08816849818060545 14-15 0.09444854116254173 16-17 0.0916225218206704 18-19 0.09481212259833803 20-21 0.1046618742226381 22-23 0.11383404226204503 24-25 0.1373180977287068 26-27 0.1423917114009553 28-29 0.14245781711655464 30-31 0.14353203499504372 32-33 0.1399953792104796 34-35 0.13487218625153158 36-37 0.1390203199053895 38-39 0.13153384761376546 40-41 0.12781540111130318 42-43 0.12755097824890588 44-45 0.11799870234480278 46-47 0.13728504487090712 48-49 0.1544064252111334 50-51 0.16357859325054033 52-53 0.1724532855697503 54-55 0.16850346906269037 56-57 0.13930126919668664 58-59 0.1317156383316636 60-61 0.12806329754480067 62-63 0.12421263961113975 64-65 0.11001643718618379 66-67 0.12414653389554041 68-69 0.12141967312706808 70-71 0.1299803632971812 72-73 0.1567697045438094 74-75 0.1696603190856786 76-77 0.17556025420291876 78-79 0.1720566512761543 80-81 0.1789977514140839 82-83 0.17790700710669496 84-85 0.15500137665152736 86-87 0.1566705459704104 88-89 0.1752627784827218 90-91 0.26318338022982973 92-93 0.526515498319758 94-95 0.1757420449208169 96-97 0.18886402946728378 98-99 0.20720836554609764 100-101 0.21469483783772172 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3025457.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.815959719135336 #Duplication Level Percentage of deduplicated Percentage of total 1 75.44521912983112 42.11047311951992 2 13.091546531712453 14.614344677504961 3 4.831936552998881 8.090975280228095 4 2.268574823725553 5.0649072392364 5 1.2755745473469504 3.559870877673583 6 0.7656380196207214 2.564089251755324 7 0.5044269358346954 1.9708551472257356 8 0.34457530073151077 1.5386240884671158 9 0.24694214698291658 1.240498163605974 >10 1.1243995817194106 10.712907303751352 >50 0.061590973435394984 2.36329931629899 >100 0.03554714071559281 3.5579079503907876 >500 0.003126453700708175 1.1847280395214876 >1k 7.214893153048736E-4 0.6601696736074254 >5k 1.2024821921747895E-4 0.40787424832374186 >10k+ 6.012410960873947E-5 0.3584756228891073 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10682 0.35307062701601777 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7046 0.2328904360564371 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5108 0.16883399764068702 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 3298 0.109008325023294 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 3.305285779966465E-5 0.0 0.0 0.0 0.0 6 3.305285779966465E-5 0.0 0.0 0.0 0.0 7 6.61057155993293E-5 0.0 0.0 0.0 0.0 8 6.61057155993293E-5 0.0 0.0 9.915857339899394E-5 0.0 9 6.61057155993293E-5 0.0 0.0 9.915857339899394E-5 0.0 10-11 6.61057155993293E-5 0.0 0.0 1.6526428899832322E-4 0.0 12-13 6.61057155993293E-5 3.305285779966465E-5 0.0 1.9831714679798787E-4 0.0 14-15 1.322114311986586E-4 3.305285779966465E-5 0.0 2.4789643349748487E-4 0.0 16-17 1.487378600984909E-4 3.305285779966465E-5 0.0 3.3052857799664644E-4 1.6526428899832324E-5 18-19 2.974757201969818E-4 3.305285779966465E-5 0.0 4.296871513956404E-4 3.305285779966465E-5 20-21 3.635814357963111E-4 1.1568500229882626E-4 0.0 6.280042981936283E-4 3.305285779966465E-5 22-23 3.635814357963111E-4 1.322114311986586E-4 0.0 0.0011568500229882626 3.305285779966465E-5 24-25 4.296871513956404E-4 1.322114311986586E-4 0.0 0.0017022221766827292 3.305285779966465E-5 26-27 4.296871513956404E-4 1.322114311986586E-4 0.0 0.0020658036124790403 6.61057155993293E-5 28-29 4.62740009195305E-4 1.322114311986586E-4 0.0 0.004115080796058249 6.61057155993293E-5 30-31 4.957928669949696E-4 1.322114311986586E-4 0.0 0.009717540193101405 6.61057155993293E-5 32-33 5.123192958948019E-4 1.6526428899832322E-4 0.0 0.020327507546793758 6.61057155993293E-5 34-35 5.453721536944667E-4 1.6526428899832322E-4 0.0 0.03584582428373631 6.61057155993293E-5 36-37 5.949514403939636E-4 1.6526428899832322E-4 3.305285779966465E-5 0.06137915693397725 6.61057155993293E-5 38-39 7.602157293922869E-4 1.6526428899832322E-4 3.305285779966465E-5 0.10153837916056979 6.61057155993293E-5 40-41 8.097950160917838E-4 1.6526428899832322E-4 3.305285779966465E-5 0.1482751200892956 6.61057155993293E-5 42-43 8.263214449916161E-4 1.8179071789815556E-4 3.305285779966465E-5 0.1938219581372335 6.61057155993293E-5 44-45 8.759007316911131E-4 1.9831714679798787E-4 3.305285779966465E-5 0.2486235963690775 6.61057155993293E-5 46-47 0.001074217878489101 1.9831714679798787E-4 3.305285779966465E-5 0.3144318362482098 6.61057155993293E-5 48-49 0.001123797165188598 1.9831714679798787E-4 3.305285779966465E-5 0.38847023771945854 6.61057155993293E-5 50-51 0.001123797165188598 1.9831714679798787E-4 3.305285779966465E-5 0.47288723653980214 6.61057155993293E-5 52-53 0.001123797165188598 1.9831714679798787E-4 3.305285779966465E-5 0.5576347639381423 6.61057155993293E-5 54-55 0.001123797165188598 2.3137000459765253E-4 3.305285779966465E-5 0.6495547614790096 6.61057155993293E-5 56-57 0.0012560085963872565 2.3137000459765253E-4 3.305285779966465E-5 0.7555056971558347 6.61057155993293E-5 58-59 0.0012560085963872565 2.644228623973172E-4 3.305285779966465E-5 0.8752562009640197 6.61057155993293E-5 60-61 0.0012560085963872565 2.644228623973172E-4 3.305285779966465E-5 1.01320230299092 6.61057155993293E-5 62-63 0.0012560085963872565 2.644228623973172E-4 3.305285779966465E-5 1.155610540820775 6.61057155993293E-5 64-65 0.0012560085963872565 2.644228623973172E-4 3.305285779966465E-5 1.298134463652929 6.61057155993293E-5 66-67 0.0012890614541869211 2.644228623973172E-4 3.305285779966465E-5 1.455945333217428 6.61057155993293E-5 68-69 0.0012890614541869211 2.644228623973172E-4 3.305285779966465E-5 1.63907469185647 6.61057155993293E-5 70-71 0.0013551671697862504 2.644228623973172E-4 3.305285779966465E-5 1.8482001231549483 6.61057155993293E-5 72-73 0.001388220027585915 2.644228623973172E-4 3.305285779966465E-5 2.068910581112209 6.61057155993293E-5 74-75 0.001388220027585915 2.644228623973172E-4 3.305285779966465E-5 2.2907944155213578 6.61057155993293E-5 76-77 0.001388220027585915 2.644228623973172E-4 3.305285779966465E-5 2.5318323810254117 6.61057155993293E-5 78-79 0.001388220027585915 2.644228623973172E-4 3.305285779966465E-5 2.8075593207902143 6.61057155993293E-5 80-81 0.0015534843165842384 2.644228623973172E-4 3.305285779966465E-5 3.109546756076851 6.61057155993293E-5 82-83 0.0015534843165842384 2.644228623973172E-4 3.305285779966465E-5 3.4185909765037152 6.61057155993293E-5 84-85 0.0016030636032837353 2.644228623973172E-4 3.305285779966465E-5 3.728758994095768 6.61057155993293E-5 86-87 0.0016526428899832322 2.644228623973172E-4 3.305285779966465E-5 4.056263235603746 6.61057155993293E-5 88-89 0.0016526428899832322 2.644228623973172E-4 3.305285779966465E-5 4.4121598819616334 6.61057155993293E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 12230 0.0 28.211624 1 TACATGG 12345 0.0 27.449501 2 GTATCAA 17560 0.0 27.270102 1 ACATGGG 12625 0.0 25.487825 3 TATCAAC 20595 0.0 23.251804 2 CATGGGA 8950 0.0 23.226213 4 TCAACGC 21025 0.0 22.88913 4 ATCAACG 20990 0.0 22.859842 3 CAACGCA 21210 0.0 22.689108 5 AACGCAG 21610 0.0 22.244226 6 GGTATCA 12575 0.0 21.097158 1 AGTACTT 9875 0.0 20.807533 12-13 GAGTACT 9475 0.0 20.108337 12-13 ACGCAGA 23440 0.0 20.102364 7 CGCAGAG 23430 0.0 19.889149 8 CATGGGG 4485 0.0 19.364931 4 ATGGGAT 4005 0.0 18.485968 5 ATGGGAG 3900 0.0 18.375216 5 GCAGAGT 23885 0.0 18.198687 9 GTACTTT 10595 0.0 18.117647 14-15 >>END_MODULE