FastQCFastQC Report
Fri 27 May 2016
SRR939643_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939643_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3025457
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT112140.3706547473654393No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83480.27592525691160047No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63370.20945595987647483No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA33930.11214834651426214No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG129000.026.3843821
GTATCAA176100.026.0908851
TACATGG127850.025.8762872
GGTATCA124650.025.152451
ACATGGG127700.024.5411423
ATCAACG203150.022.3986113
TCAACGC203600.022.3953274
CAACGCA206800.022.0487845
AACGCAG207750.021.9605486
CATGGGA87450.021.8462894
TATCAAC210600.021.7421172
GAGTACT97300.020.49886512-13
AGTACTT101550.019.82603512-13
CGCAGAG227950.019.5610268
ACGCAGA229700.019.5347277
GTACTTT108550.018.50424214-15
CATGGGG48750.018.2430974
ATGGGAG38700.018.1170185
GCAGAGT235200.017.9991689
AGAGTAC195600.017.25928910-11
Produced by FastQC (version 0.11.5)