##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939643_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3025457 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.523972411440653 31.0 31.0 34.0 30.0 34.0 2 31.722439948741627 31.0 31.0 34.0 30.0 34.0 3 31.769899225141856 31.0 31.0 34.0 30.0 34.0 4 35.5635254442552 37.0 35.0 37.0 33.0 37.0 5 35.292418302425055 37.0 35.0 37.0 33.0 37.0 6 35.31608646230966 37.0 35.0 37.0 32.0 37.0 7 35.24041227490591 37.0 35.0 37.0 32.0 37.0 8 35.20227126017656 37.0 35.0 37.0 32.0 37.0 9 36.81143113255287 39.0 37.0 39.0 32.0 39.0 10-11 36.689283470232766 39.0 35.0 39.0 32.0 39.0 12-13 36.630789827784696 39.0 35.0 39.0 32.0 39.0 14-15 37.67759713656483 39.0 36.5 41.0 32.0 41.0 16-17 37.639450502849655 39.0 36.0 41.0 32.0 41.0 18-19 37.62560152069588 39.0 36.5 41.0 32.0 41.0 20-21 37.56966005466282 39.0 36.0 41.0 32.0 41.0 22-23 37.43077624306014 39.0 36.0 41.0 32.0 41.0 24-25 37.30385459122374 39.0 36.0 41.0 31.0 41.0 26-27 37.04235194881302 39.0 36.0 40.0 31.0 41.0 28-29 36.833724458817294 39.0 36.0 40.0 30.5 41.0 30-31 36.59442193361201 38.5 35.0 40.0 30.0 41.0 32-33 36.42757061164644 38.0 35.0 40.0 30.0 41.0 34-35 36.40819205164708 38.0 35.0 40.0 30.0 41.0 36-37 36.29652809476387 38.0 35.0 40.0 30.0 41.0 38-39 36.33080489988785 38.0 35.0 40.0 30.0 41.0 40-41 36.227327144295884 38.0 35.0 40.0 30.0 41.0 42-43 36.04352532526491 38.0 35.0 40.0 29.5 41.0 44-45 35.83472067195138 38.0 35.0 40.0 29.0 41.0 46-47 35.523431997215624 38.0 34.5 40.0 27.5 41.0 48-49 35.22884377467602 38.0 34.0 40.0 27.0 41.0 50-51 34.979356011339775 38.0 34.0 40.0 26.0 41.0 52-53 34.72854134102716 38.0 34.0 40.0 26.0 41.0 54-55 34.439345526973284 38.0 33.0 40.0 25.0 41.0 56-57 32.411126980155395 37.0 30.5 39.5 16.0 41.0 58-59 27.365274733701387 32.5 17.5 38.5 6.5 40.0 60-61 28.100477051896622 32.5 19.0 38.0 8.0 40.0 62-63 31.182493421654975 34.5 29.0 38.5 10.0 40.0 64-65 31.405436104363737 35.0 30.5 39.0 5.0 40.0 66-67 24.71166537815609 27.0 18.0 35.5 2.0 38.5 68-69 17.278350510352652 13.0 7.0 28.0 2.0 35.0 70-71 16.147788251493907 12.5 7.0 26.5 2.0 33.5 72-73 17.238912666747538 13.0 7.0 28.5 2.0 34.0 74-75 18.2332184856701 17.0 8.5 29.0 2.0 34.5 76-77 19.455814939693408 21.0 8.0 30.0 2.0 34.5 78-79 22.51105386723394 27.5 10.5 33.5 2.0 35.5 80-81 26.150096167289767 31.5 22.5 34.5 2.0 36.0 82-83 27.465112873856746 33.0 26.5 35.0 2.0 36.0 84-85 27.520712077547294 34.0 27.0 35.0 2.0 36.0 86-87 27.27411032448982 33.5 26.0 35.0 2.0 36.0 88-89 26.85154391551425 33.0 26.0 35.0 2.0 35.0 90-91 25.497785458527424 32.5 16.5 35.0 2.0 35.0 92-93 22.999795072281643 31.5 2.0 34.0 2.0 35.0 94-95 15.691503134898298 4.5 2.0 32.0 2.0 34.5 96-97 13.137422544759353 2.0 2.0 30.0 2.0 34.0 98-99 12.80997895524544 2.0 2.0 29.5 2.0 34.0 100-101 12.520449307327786 2.0 2.0 30.5 2.0 34.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.3667453351093748 1101 2 0.36598778176468016 1101 3 0.3408519229580591 1101 4 0.23502346888938774 1101 5 0.3528464856801108 1101 6 0.37954379606448896 1101 7 0.42281932365371233 1101 8 0.4022871065134126 1101 9 0.3995958369097252 1101 10-11 0.43590239393465424 1101 12-13 0.4606382532874491 1101 14-15 0.6202281093460726 1101 16-17 0.5641052952385834 1101 18-19 0.462313011628261 1101 20-21 0.45612393839335397 1101 22-23 0.6097743237624229 1101 24-25 0.5377038551875444 1101 26-27 0.5777011047733325 1101 28-29 0.449665094336261 1101 30-31 0.6032383187482964 1101 32-33 0.6460642000062506 1101 34-35 0.46138022577171256 1101 36-37 0.21487739209936763 1101 38-39 0.39038105784617017 1101 40-41 0.4336869072136835 1101 42-43 0.5040137705454981 1101 44-45 0.2657712505385419 1101 46-47 0.3398783540360455 1101 48-49 0.4395232761158141 1101 50-51 0.3934568536944809 1101 52-53 0.19428447790222947 1101 54-55 0.17777231887317413 1101 56-57 -2.457495917775738 1101 58-59 -10.267503234273303 1101 60-61 -10.232722968497566 1101 62-63 -4.160174474941758 1101 64-65 -5.510268413787969 1101 66-67 -10.106942127186734 1101 68-69 -5.2271106075924045 1101 70-71 -4.769057341273234 1101 72-73 -4.980861839696054 1101 74-75 -6.740408275766374 1101 76-77 -8.194901040715639 1101 78-79 -11.097677242612308 1101 80-81 -8.259774767954656 1101 82-83 -4.661801302579377 1101 84-85 -3.8949299081929247 1101 86-87 -3.635624074977134 1101 88-89 -3.6931833813374375 1101 90-91 -9.984836179668036 1101 92-93 -17.077397731036772 1101 94-95 -11.349648986268964 1101 96-97 -9.351211690647053 1101 98-99 -9.405199991659927 1101 100-101 -9.335401825280481 1102 1 0.30893544287775754 1102 2 0.2533557265984747 1102 3 0.26761236666227006 1102 4 0.196471197118548 1102 5 0.19803370963522582 1102 6 0.2040906274581502 1102 7 0.28753300055295483 1102 8 0.34719840758225473 1102 9 0.36983910949523846 1102 10-11 0.39364969254855 1102 12-13 0.3315258195913273 1102 14-15 0.3045769347791989 1102 16-17 0.39875982612087313 1102 18-19 0.33719782846333857 1102 20-21 0.3989696213713856 1102 22-23 0.36235794315189196 1102 24-25 0.28588116233863303 1102 26-27 0.38282611690165425 1102 28-29 0.40997613337596306 1102 30-31 0.3411168874169874 1102 32-33 0.30226225993421707 1102 34-35 0.270022020338331 1102 36-37 0.08694205140694322 1102 38-39 0.17025318372120068 1102 40-41 0.0742595275797342 1102 42-43 0.2091022398279705 1102 44-45 0.3284723725445673 1102 46-47 0.37501955352553296 1102 48-49 0.3081406934752948 1102 50-51 0.2834049014223652 1102 52-53 0.07519319484932652 1102 54-55 -0.08901930538025482 1102 56-57 -2.892195029207592 1102 58-59 -10.529960598560983 1102 60-61 -10.723646391571858 1102 62-63 -4.582004935657196 1102 64-65 -5.5997635799703644 1102 66-67 -6.91175819007832 1102 68-69 -4.148165788724928 1102 70-71 -4.23538072622585 1102 72-73 -4.681567822790466 1102 74-75 -5.324585855051145 1102 76-77 -7.420371332501507 1102 78-79 -10.04937186326427 1102 80-81 -6.071411970695973 1102 82-83 -3.480341457511855 1102 84-85 -2.8974922133144894 1102 86-87 -2.896563102679078 1102 88-89 -3.037465574206731 1102 90-91 -8.714422924001884 1102 92-93 -13.064074017112425 1102 94-95 -10.42451804346354 1102 96-97 -9.159117461201614 1102 98-99 -9.266972801945432 1102 100-101 -9.219183375420538 1103 1 0.19591824284114523 1103 2 0.2374214813063773 1103 3 0.25022761522445336 1103 4 0.16301213283343685 1103 5 0.21288699623382001 1103 6 0.21872091331493948 1103 7 0.17931214240887527 1103 8 0.25518733553062845 1103 9 0.4363291426107949 1103 10-11 0.2954441358130069 1103 12-13 0.29820075601609375 1103 14-15 0.45843578414485364 1103 16-17 0.38605626431721163 1103 18-19 0.36744199483815265 1103 20-21 0.26112088231512587 1103 22-23 0.35706677050607993 1103 24-25 0.45465102168559923 1103 26-27 0.471656807269774 1103 28-29 0.4194566099731887 1103 30-31 0.4253536800419653 1103 32-33 0.3466646762585768 1103 34-35 0.3648701377491079 1103 36-37 0.18079585409970633 1103 38-39 0.1329348989915431 1103 40-41 0.27698669824692246 1103 42-43 0.2286464347935322 1103 44-45 0.25987078274836506 1103 46-47 0.1233973203162293 1103 48-49 -0.0034706241245245906 1103 50-51 0.1674539399098407 1103 52-53 0.4176637862583661 1103 54-55 0.25394075675367134 1103 56-57 -2.7579819797413627 1103 58-59 -10.375562212271156 1103 60-61 -10.613124667612379 1103 62-63 -4.334380635715188 1103 64-65 -5.537147551553499 1103 66-67 -6.742076772495707 1103 68-69 -4.520653187062566 1103 70-71 -4.231807884441283 1103 72-73 -4.601792458635439 1103 74-75 -5.206222645155144 1103 76-77 -6.950763577023412 1103 78-79 -9.632053069626423 1103 80-81 -5.432452822240737 1103 82-83 -3.196078359516754 1103 84-85 -2.7561179303066403 1103 86-87 -2.584425736578183 1103 88-89 -2.623723156438107 1103 90-91 -5.625958070297084 1103 92-93 -10.74938345155168 1103 94-95 -8.876218945397286 1103 96-97 -8.178702003633662 1103 98-99 -8.442983588443013 1103 100-101 -8.433320161695944 1104 1 0.25545311964454953 1104 2 0.28179082360720287 1104 3 0.17147308794419658 1104 4 0.21424051182022907 1104 5 0.2813671303328533 1104 6 0.24706943299722894 1104 7 0.34488043657986367 1104 8 0.27879333127420125 1104 9 0.21097751049338598 1104 10-11 0.28619202025713975 1104 12-13 0.35286740197766164 1104 14-15 0.4391445042451778 1104 16-17 0.37562220860571216 1104 18-19 0.4206887378592299 1104 20-21 0.41609529420748714 1104 22-23 0.5467089481848859 1104 24-25 0.49042455985210154 1104 26-27 0.45266839406708215 1104 28-29 0.5654414746214727 1104 30-31 0.5064075664740102 1104 32-33 0.4569702692479538 1104 34-35 0.5317304954784774 1104 36-37 0.43972325871856555 1104 38-39 0.4176633857505436 1104 40-41 0.4223464184744259 1104 42-43 0.511898316542208 1104 44-45 0.4469060868677559 1104 46-47 0.28525553845833684 1104 48-49 0.36945254637526403 1104 50-51 0.3630181758092661 1104 52-53 0.3984856917825468 1104 54-55 0.3498867282657159 1104 56-57 -2.7941417153227164 1104 58-59 -10.485120234673559 1104 60-61 -10.426620842874595 1104 62-63 -4.202075623853684 1104 64-65 -5.52353823461058 1104 66-67 -6.666228650055981 1104 68-69 -3.905875949646461 1104 70-71 -3.6763324821491192 1104 72-73 -4.178967803197569 1104 74-75 -4.88923106341673 1104 76-77 -6.656563493408363 1104 78-79 -9.304596976002065 1104 80-81 -5.119941961634549 1104 82-83 -2.7745652333430044 1104 84-85 -2.1275375390743854 1104 86-87 -2.0020755216297665 1104 88-89 -1.9340100601628052 1104 90-91 -4.503935434743326 1104 92-93 -6.849703301530143 1104 94-95 -7.5707508427447765 1104 96-97 -7.680976647900623 1104 98-99 -8.059458552538082 1104 100-101 -8.02999401937342 1105 1 0.295517201018054 1105 2 0.31676765204093726 1105 3 0.2686240442116201 1105 4 0.2002490217537911 1105 5 0.23329005926911606 1105 6 0.27807385357584735 1105 7 0.2615401846894727 1105 8 0.2525914370130238 1105 9 0.41049500345975787 1105 10-11 0.4363117203263158 1105 12-13 0.29667892791076866 1105 14-15 0.5507236867513612 1105 16-17 0.5011275252006868 1105 18-19 0.49583423890763356 1105 20-21 0.5021537261365907 1105 22-23 0.513436252481668 1105 24-25 0.5417594584051031 1105 26-27 0.5809225635725284 1105 28-29 0.6717335427902427 1105 30-31 0.6372181518446354 1105 32-33 0.6133468016180217 1105 34-35 0.5582122932425833 1105 36-37 0.55403882989404 1105 38-39 0.5542211243426749 1105 40-41 0.6027074566004842 1105 42-43 0.6523296161376919 1105 44-45 0.47247506863897115 1105 46-47 0.6566546462787954 1105 48-49 0.6441223686265118 1105 50-51 0.5606321623786599 1105 52-53 0.6172289516086593 1105 54-55 0.5792086605395852 1105 56-57 1.6742665815698743 1105 58-59 -8.482521386196026 1105 60-61 -9.662382398255819 1105 62-63 -3.435594645477952 1105 64-65 -4.695035408912911 1105 66-67 -6.102728001807055 1105 68-69 -3.8091675281331376 1105 70-71 -3.8022069028599628 1105 72-73 -2.2477280902609635 1105 74-75 -4.5189022003744626 1105 76-77 -6.506476417135662 1105 78-79 -8.857585879359107 1105 80-81 -4.205337295933081 1105 82-83 -1.9550145508119954 1105 84-85 -1.4236375105258539 1105 86-87 -1.3383273118741492 1105 88-89 -1.479371173074309 1105 90-91 -4.160605472896908 1105 92-93 -6.654357102354169 1105 94-95 -7.838043080644003 1105 96-97 -8.09673647779733 1105 98-99 -8.488060073348919 1105 100-101 -8.429676170384964 1106 1 0.1780135184737226 1106 2 0.18694677425257566 1106 3 0.179970349866867 1106 4 0.13946059104666375 1106 5 0.04979762374416197 1106 6 0.09277066420761315 1106 7 0.18307147207442398 1106 8 0.1376594318371147 1106 9 0.17731466865340906 1106 10-11 0.257252579885062 1106 12-13 0.20660511425971606 1106 14-15 0.33386658574791994 1106 16-17 0.34218199430393526 1106 18-19 0.21901677103326023 1106 20-21 0.24997471786002023 1106 22-23 0.3412762876387774 1106 24-25 0.3625111043600029 1106 26-27 0.3373110658959959 1106 28-29 0.3579990766167569 1106 30-31 0.30254608140020167 1106 32-33 0.16034705390283222 1106 34-35 0.3362118375022902 1106 36-37 0.21951121284610053 1106 38-39 0.2821665494425716 1106 40-41 0.2774146841532428 1106 42-43 0.20244680649878433 1106 44-45 0.18232516263540788 1106 46-47 0.16967783206620624 1106 48-49 0.13716419093662324 1106 50-51 0.07426164552034464 1106 52-53 0.09929841945048423 1106 54-55 0.07785568154432809 1106 56-57 1.4984667980465076 1106 58-59 -8.56777737389104 1106 60-61 -9.816735938788224 1106 62-63 -3.836131361055319 1106 64-65 -5.428046875664219 1106 66-67 -6.9425963254152165 1106 68-69 -4.297306073182252 1106 70-71 -4.278207293494498 1106 72-73 -4.7483811823774715 1106 74-75 -5.097228494147911 1106 76-77 -6.656014166266907 1106 78-79 -9.266049220749277 1106 80-81 -4.988474091265001 1106 82-83 -2.5522601704153374 1106 84-85 -2.1540956109026546 1106 86-87 -2.0136713551043677 1106 88-89 -1.8145824005619566 1106 90-91 -4.325105338616286 1106 92-93 -6.603436262815444 1106 94-95 -7.524320821799794 1106 96-97 -7.464868837799608 1106 98-99 -6.957694341763677 1106 100-101 -7.458611609889509 1107 1 0.0060631205292445145 1107 2 -0.08175551517729218 1107 3 -0.04366995710677202 1107 4 0.033718850998660344 1107 5 -0.10300979450318692 1107 6 -0.08335491145264484 1107 7 -0.053196521807784336 1107 8 -0.01072277016184131 1107 9 0.0328908455070831 1107 10-11 -0.032626501819322584 1107 12-13 -0.007343023810669536 1107 14-15 0.030561853350548063 1107 16-17 0.03044782993652717 1107 18-19 -0.021345842002794768 1107 20-21 -0.04031525485381593 1107 22-23 0.039157226953726365 1107 24-25 0.12758195990251409 1107 26-27 0.02977727364349647 1107 28-29 -0.10188561772225313 1107 30-31 0.041165358460681034 1107 32-33 0.10585305059326089 1107 34-35 0.014739879080245544 1107 36-37 0.06818989364195716 1107 38-39 -0.060223482298134456 1107 40-41 -0.045379662647029306 1107 42-43 0.012051938280315255 1107 44-45 -0.17965610797530474 1107 46-47 -0.1276144369371508 1107 48-49 -0.3967120075969319 1107 50-51 -0.30325959399328184 1107 52-53 -0.31804542608710307 1107 54-55 -0.5323723552911588 1107 56-57 0.77077236291764 1107 58-59 -9.257859746873159 1107 60-61 -10.901676950019418 1107 62-63 -4.635195865492072 1107 64-65 -6.031955140951499 1107 66-67 -7.421842650843857 1107 68-69 -4.150930425114556 1107 70-71 -4.092814889669354 1107 72-73 -2.612743323706617 1107 74-75 -4.9200565040529085 1107 76-77 -6.95093101628105 1107 78-79 -7.835494141558412 1107 80-81 -2.860200830694783 1107 82-83 -1.8142843032622302 1107 84-85 -1.5053352282695975 1107 86-87 -1.4847232551431553 1107 88-89 -1.2278396722160139 1107 90-91 -0.792759064677373 1107 92-93 -3.535504646766231 1107 94-95 -6.290562475890432 1107 96-97 -6.5122809919165 1107 98-99 -6.1974956913366 1107 100-101 -6.917591158388939 1108 1 -0.055451950372791714 1108 2 -0.046265116126296135 1108 3 -0.08024373798074436 1108 4 -0.025075910834146953 1108 5 0.034977124545363836 1108 6 -0.16742831458963536 1108 7 -0.08586434150990385 1108 8 -0.06748840123978539 1108 9 -0.08465809152497172 1108 10-11 -0.15297340501899725 1108 12-13 -0.12778616957213984 1108 14-15 -0.22479990214592505 1108 16-17 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1.657173986868731 2316 90-91 2.6615316800818007 2316 92-93 4.712381488778387 2316 94-95 11.699073717777951 2316 96-97 13.447559264603049 2316 98-99 12.988729945118626 2316 100-101 11.908358922984332 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6.0 3 21.0 4 62.0 5 110.0 6 282.0 7 727.0 8 1590.0 9 2620.0 10 4390.0 11 7288.0 12 10940.0 13 15072.0 14 18771.0 15 25450.0 16 34689.0 17 46930.0 18 51569.0 19 51414.0 20 53086.0 21 53076.0 22 53165.0 23 45628.0 24 45951.0 25 54212.0 26 76678.0 27 113939.0 28 160014.0 29 216762.0 30 267982.0 31 291912.0 32 289469.0 33 264395.0 34 234072.0 35 210261.0 36 169642.0 37 109368.0 38 41241.0 39 2673.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.711719949986346 19.62530093157373 14.082043815504894 23.58093530293503 2 15.4264532439165 21.41333292785479 39.57260137404338 23.587612454185333 3 18.865201718485885 25.840518480862706 30.529434590643255 24.76484521000815 4 12.599947710378961 17.404709437285014 37.76768270049781 32.227660151838215 5 13.028065168626352 38.49412160348371 34.62297597568076 13.854837252209176 6 31.04456615975702 37.32077501018855 17.81668025690003 13.817978573154402 7 27.42439486807939 33.39734572242994 21.544157310501294 17.634102098989377 8 25.833386769334304 35.43345631325969 20.250503565448305 18.48265335195771 9 26.538271738781944 16.154518143870497 20.329755141124135 36.97745497622343 10-11 24.451545667315713 26.81161887278517 28.196665826022315 20.540169633876797 12-13 25.793144638975203 24.126586495858312 28.385744699065295 21.694524166101186 14-15 22.54295080580068 25.263218086108523 26.80682929215538 25.387001815935413 16-17 21.907020988895233 28.645275738508264 27.28946403799492 22.15823923460158 18-19 21.727825612718767 27.451833611528865 29.4322166950371 21.388124080715265 20-21 22.700289576087183 26.542072817428902 29.1741710425896 21.583466563894312 22-23 22.276913883110126 26.703352166920187 28.94155109692918 22.07818285304051 24-25 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28.084567792466924 22.33581424668005 50-51 22.422140828982318 27.29538918583193 28.016330414934558 22.266139570251195 52-53 22.585973524671772 27.227864845449208 27.899664431028892 22.28649719885013 54-55 22.11705473101552 27.07953282104495 28.157822574803177 22.645589873136352 56-57 21.540155759419395 27.43661991491161 28.753376266380315 22.269848059288684 58-59 21.21284969180502 26.711843191817863 29.899907349288146 22.17539976708897 60-61 22.226377149281838 26.970076385131502 28.578697650203246 22.22484881538341 62-63 22.493296550301658 27.060186929727486 28.093404043489123 22.353112476481733 64-65 21.94866176059131 27.0804340117863 28.42209014561867 22.54881408200372 66-67 21.78194907417545 26.855531618512785 29.14902970757318 22.213489599738583 68-69 22.016514384428906 26.35252686002848 29.655420755516758 21.975538000025853 70-71 21.84820310282769 26.47542362655944 29.482352221415937 22.194021049196934 72-73 21.06279945672721 26.569070103502835 29.953783802678764 22.414346637091196 74-75 21.67800436691016 26.70799420420743 29.139522425478148 22.47447900340426 76-77 19.94834250161744 27.21294219790755 29.77129572828357 23.06741957219144 78-79 20.648863232412435 27.239584697371587 29.081342859206565 23.030209211009414 80-81 21.597490535023926 27.435971588454226 28.008723138888158 22.95781473763369 82-83 22.031942019874396 27.340556389866567 27.5809833940433 23.046518196215736 84-85 21.98437358234063 27.13642764630162 27.493580714428433 23.385618056929317 86-87 22.065532795649702 27.038880535669605 27.74816415557767 23.147422513103024 88-89 22.035023503910487 26.963606123246297 27.627837012230234 23.373533360612985 90-91 21.260601349497616 27.17830479136597 28.183287541600233 23.37780631753618 92-93 20.363743209325968 27.313524407935514 28.83479798201011 23.48793440072841 94-95 19.585092001514727 27.135430910094556 29.414772779003144 23.864704309387573 96-97 19.60196390122517 27.14121855885303 29.06099364082335 24.195823899098446 98-99 19.813523095642665 27.065518182956495 28.73973373804159 24.381224983359257 100-101 20.23945321218822 27.007407031759058 28.503626779817882 24.24951297623484 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 529.0 1 417.5 2 328.5 3 368.5 4 416.5 5 505.5 6 696.0 7 993.5 8 1354.0 9 1664.5 10 1856.5 11 2038.0 12 2328.0 13 2714.0 14 3097.0 15 3422.0 16 3735.5 17 4111.0 18 4486.0 19 5029.5 20 5605.5 21 6386.0 22 7664.5 23 9222.0 24 11317.5 25 14140.5 26 17642.0 27 22042.5 28 26976.0 29 32164.5 30 37772.5 31 43758.0 32 50063.5 33 56334.0 34 62729.0 35 69212.5 36 75608.5 37 81543.5 38 86443.5 39 90688.5 40 95124.0 41 100054.0 42 104512.5 43 108955.0 44 113047.0 45 116608.0 46 120449.5 47 124383.5 48 128087.5 49 127354.5 50 121761.5 51 115735.5 52 109926.0 53 103588.5 54 96330.0 55 87421.5 56 77326.5 57 67333.5 58 57828.0 59 48473.0 60 39545.5 61 31336.0 62 24029.0 63 17931.5 64 13000.5 65 9168.0 66 6335.5 67 4349.0 68 2878.5 69 1839.0 70 1220.5 71 835.0 72 564.5 73 395.5 74 258.5 75 146.5 76 77.5 77 40.0 78 24.0 79 14.5 80 9.0 81 6.0 82 6.0 83 3.5 84 1.0 85 0.0 86 0.5 87 1.0 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.022575101877170954 2 0.007436893004924545 3 9.915857339899395E-5 4 0.0 5 0.14245781711655464 6 0.0 7 3.305285779966465E-5 8 0.001024638591789604 9 0.0 10-11 0.0 12-13 0.0 14-15 6.280042981936284E-4 16-17 0.0 18-19 2.3137000459765253E-4 20-21 0.0 22-23 3.635814357963111E-4 24-25 0.0 26-27 2.3137000459765253E-4 28-29 0.002826019341871327 30-31 0.013088931688667198 32-33 0.0024459114771751837 34-35 0.00404897508045892 36-37 0.10943801217468965 38-39 0.10841337358290003 40-41 0.12160146384496623 42-43 0.12396474317764225 44-45 0.11953566023248718 46-47 0.11219792580096165 48-49 0.111867397222965 50-51 0.1112559193536712 52-53 0.08859818533200108 54-55 0.07569104436123203 56-57 0.5482146994652378 58-59 1.555682331627916 60-61 0.5170623809890539 62-63 0.0049248758121500326 64-65 0.11758554162230699 66-67 1.8847400574524775 68-69 2.8411906036013734 70-71 2.753369160427664 72-73 2.60691194751735 74-75 1.989864671684311 76-77 1.3996893692423988 78-79 0.9280085620122844 80-81 0.05108319172938171 82-83 2.1484357569782022E-4 84-85 1.1568500229882626E-4 86-87 3.305285779966465E-4 88-89 9.915857339899395E-5 90-91 0.2460785263185033 92-93 0.38886687201305453 94-95 1.9801306050623098 96-97 2.398282309085867 98-99 1.406977524387225 100-101 0.9310990042165531 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3025457.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.66010018718602 #Duplication Level Percentage of deduplicated Percentage of total 1 70.8245475485816 35.171541269746825 2 14.920195775126418 14.81876834010415 3 5.748761213900201 8.564521735034793 4 2.8275052629742987 5.616567785563976 5 1.6372974188401537 4.065417692791154 6 0.9845931697466759 2.9336997271943384 7 0.6513537772690708 2.264240568553887 8 0.44074520982933807 1.75099610217178 9 0.3256569973348605 1.455494320287661 >10 1.5000392413635286 12.821898766862283 >50 0.08457732812100932 2.9057617623511947 >100 0.04945312914164473 4.475217667282023 >500 0.003938756183754901 1.357954530884175 >1k 0.0011348958493443801 0.9393063088819246 >5k 1.3351715874639766E-4 0.48684266212300364 >10k+ 6.675857937319883E-5 0.37177076016665733 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11214 0.3706547473654393 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8348 0.27592525691160047 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6337 0.20945595987647483 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 3393 0.11214834651426214 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 3.305285779966465E-5 0.0 0.0 0.0 3 0.0 3.305285779966465E-5 0.0 0.0 0.0 4 0.0 3.305285779966465E-5 0.0 0.0 0.0 5 0.0 3.305285779966465E-5 0.0 0.0 0.0 6 0.0 3.305285779966465E-5 0.0 0.0 0.0 7 1.6526428899832322E-4 3.305285779966465E-5 0.0 0.0 0.0 8 1.6526428899832322E-4 3.305285779966465E-5 0.0 0.0 0.0 9 1.6526428899832322E-4 3.305285779966465E-5 0.0 0.0 0.0 10-11 1.9831714679798787E-4 3.305285779966465E-5 0.0 9.915857339899394E-5 0.0 12-13 1.9831714679798787E-4 3.305285779966465E-5 0.0 1.3221143119865856E-4 0.0 14-15 3.140021490968141E-4 3.305285779966465E-5 0.0 1.8179071789815556E-4 0.0 16-17 3.3052857799664644E-4 3.305285779966465E-5 0.0 2.809492912971495E-4 0.0 18-19 3.635814357963111E-4 3.305285779966465E-5 0.0 3.635814357963111E-4 0.0 20-21 3.9663429359597575E-4 6.61057155993293E-5 0.0 5.288457247946343E-4 0.0 22-23 3.9663429359597575E-4 6.61057155993293E-5 0.0 0.0011237971651885978 0.0 24-25 6.280042981936283E-4 8.263214449916162E-5 0.0 0.0016691693188830646 0.0 26-27 6.280042981936283E-4 9.915857339899394E-5 0.0 0.001999697896879711 0.0 28-29 7.767421582921192E-4 9.915857339899394E-5 0.0 0.004181186511657577 0.0 30-31 8.593743027912808E-4 9.915857339899394E-5 0.0 0.010064595199997885 0.0 32-33 8.593743027912808E-4 9.915857339899394E-5 0.0 0.021319093280783696 0.0 34-35 8.924271605909454E-4 9.915857339899394E-5 0.0 0.03771331074941736 0.0 36-37 9.915857339899393E-4 9.915857339899394E-5 0.0 0.06403991198685025 0.0 38-39 0.001123797165188598 2.148435756978202E-4 0.0 0.10608314710802369 0.0 40-41 0.001123797165188598 2.3137000459765253E-4 0.0 0.15463779521573107 0.0 42-43 0.0012560085963872565 2.3137000459765253E-4 0.0 0.20178769686695266 0.0 44-45 0.001388220027585915 2.3137000459765253E-4 0.0 0.2581097665575812 0.0 46-47 0.0014873786009849091 2.3137000459765253E-4 0.0 0.32325694928072024 0.0 48-49 0.0014873786009849091 2.3137000459765253E-4 0.0 0.3857764298087859 0.0 50-51 0.0014873786009849091 2.3137000459765253E-4 0.0 0.449832868224536 0.0 52-53 0.0014873786009849091 2.3137000459765253E-4 0.0 0.5131290909108938 0.0 54-55 0.0015204314587845737 2.3137000459765253E-4 0.0 0.5819616672786954 0.0 56-57 0.001586537174383903 2.3137000459765253E-4 0.0 0.6389117412675176 0.0 58-59 0.001586537174383903 2.3137000459765253E-4 0.0 0.6795006506455057 0.0 60-61 0.001586537174383903 2.3137000459765253E-4 0.0 0.7135120413213607 0.0 62-63 0.0016195900321835676 2.3137000459765253E-4 0.0 0.744829624086543 0.0 64-65 0.0016195900321835676 2.3137000459765253E-4 0.0 0.7748250925397386 0.0 66-67 0.0016195900321835676 2.3137000459765253E-4 0.0 0.8005567423367775 0.0 68-69 0.0016195900321835676 2.3137000459765253E-4 0.0 0.8348821351617293 0.0 70-71 0.0016361164610834 2.3137000459765253E-4 0.0 0.8922453698730473 0.0 72-73 0.0016691693188830646 2.3137000459765253E-4 0.0 0.9764805779754926 0.0 74-75 0.0016856957477828969 2.3137000459765253E-4 0.0 1.0809276086224329 0.0 76-77 0.0016856957477828969 2.3137000459765253E-4 0.0 1.2501747669856158 0.0 78-79 0.0016856957477828969 2.3137000459765253E-4 0.0 1.5090282228436893 0.0 80-81 0.0017187486055825615 2.3137000459765253E-4 0.0 1.8231295305139026 0.0 82-83 0.0017848543211818908 2.3137000459765253E-4 0.0 2.1447173104757398 0.0 84-85 0.0017848543211818908 2.644228623973172E-4 0.0 2.3900852003515505 0.0 86-87 0.0017848543211818908 2.974757201969818E-4 0.0 2.5770156376375537 0.0 88-89 0.0017848543211818908 2.974757201969818E-4 0.0 2.7543276933038543 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 12900 0.0 26.384382 1 GTATCAA 17610 0.0 26.090885 1 TACATGG 12785 0.0 25.876287 2 GGTATCA 12465 0.0 25.15245 1 ACATGGG 12770 0.0 24.541142 3 ATCAACG 20315 0.0 22.398611 3 TCAACGC 20360 0.0 22.395327 4 CAACGCA 20680 0.0 22.048784 5 AACGCAG 20775 0.0 21.960548 6 CATGGGA 8745 0.0 21.846289 4 TATCAAC 21060 0.0 21.742117 2 GAGTACT 9730 0.0 20.498865 12-13 AGTACTT 10155 0.0 19.826035 12-13 CGCAGAG 22795 0.0 19.561026 8 ACGCAGA 22970 0.0 19.534727 7 GTACTTT 10855 0.0 18.504242 14-15 CATGGGG 4875 0.0 18.243097 4 ATGGGAG 3870 0.0 18.117018 5 GCAGAGT 23520 0.0 17.999168 9 AGAGTAC 19560 0.0 17.259289 10-11 >>END_MODULE