FastQCFastQC Report
Fri 27 May 2016
SRR939642_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939642_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3030665
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47400.1564013178625813No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30410.10034101426584593No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG108750.034.070041
GTATCAA149950.031.9958571
TACATGG129950.028.39652
ACATGGG127600.028.1793673
GGTATCA110950.026.3216511
TATCAAC194100.025.876042
ATCAACG207400.024.3082073
CATGGGA88300.024.1299084
TCAACGC210350.023.8967514
CAACGCA211600.023.8044575
AACGCAG215200.023.669486
ACGCAGA224200.022.3824257
GAGTACT89450.022.11423312-13
AGTACTT95950.021.3049812-13
CGCAGAG233600.021.0984978
CATGGGG49650.020.8384134
GTACTTT99450.019.93970114-15
GCAGAGT234450.019.3510349
ATGGGAG41750.018.7698655
ATGGGAT38750.018.6392845
Produced by FastQC (version 0.11.5)