##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939642_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3030665 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 15.37429079096502 2.0 2.0 31.0 2.0 33.0 2 22.5832723181216 16.0 16.0 31.0 16.0 34.0 3 26.965633945025267 28.0 25.0 31.0 16.0 34.0 4 32.16660831863634 35.0 32.0 35.0 25.0 37.0 5 32.780198075339904 35.0 33.0 35.0 26.0 37.0 6 33.158513065614315 35.0 33.0 37.0 26.0 37.0 7 33.31242978026275 35.0 33.0 37.0 26.0 37.0 8 33.24557019664001 35.0 33.0 37.0 26.0 37.0 9 34.572661115629735 37.0 34.0 39.0 27.0 39.0 10-11 34.4127420879576 37.0 33.0 39.0 27.0 39.0 12-13 34.414171972157924 37.0 33.0 39.0 27.0 39.0 14-15 35.07958962801894 38.0 33.0 40.0 26.5 41.0 16-17 34.69486762806183 38.0 33.0 40.0 25.0 41.0 18-19 34.941737044510035 38.0 33.0 40.0 25.0 41.0 20-21 34.933392836225714 38.0 33.5 40.0 25.0 41.0 22-23 34.7455037095819 38.0 33.0 40.0 25.0 41.0 24-25 34.5506949794847 38.0 33.0 40.0 24.0 41.0 26-27 34.637079980796294 38.0 33.0 40.0 24.0 41.0 28-29 34.37096709798014 38.0 33.0 40.0 24.0 41.0 30-31 34.22991637148942 38.0 33.0 40.0 23.5 41.0 32-33 33.91463045239246 38.0 32.0 40.0 22.0 41.0 34-35 33.63230198652771 38.0 32.0 40.0 19.0 41.0 36-37 33.344408900356854 37.0 31.0 40.0 18.0 41.0 38-39 33.13666241567445 37.0 31.0 40.0 17.0 41.0 40-41 32.739124086627854 37.0 30.5 40.0 15.5 41.0 42-43 32.47547138994247 37.0 30.0 40.0 15.0 41.0 44-45 32.11425809187092 37.0 30.0 39.5 11.0 41.0 46-47 31.76297182301574 36.0 30.0 39.0 9.0 40.0 48-49 31.38632379362285 36.0 29.5 39.0 8.5 40.0 50-51 30.735445686012806 35.5 28.5 38.5 8.0 40.0 52-53 30.771422443589113 35.5 28.5 38.5 7.0 40.0 54-55 30.87968251192395 36.0 28.5 39.0 2.0 40.0 56-57 30.58683028312268 35.5 28.0 39.0 2.0 40.0 58-59 30.16699107291634 35.0 28.0 38.5 2.0 40.0 60-61 29.574053054362658 34.5 26.5 38.0 2.0 40.0 62-63 29.165756195422457 34.0 26.0 38.0 2.0 40.0 64-65 28.581458524779215 34.0 26.0 38.0 2.0 39.5 66-67 27.97489098927133 33.0 25.0 37.0 2.0 39.0 68-69 27.617400966454557 33.0 24.5 36.5 2.0 39.0 70-71 27.083126640522792 32.5 22.5 36.0 2.0 39.0 72-73 26.478169477655896 32.0 22.0 35.5 2.0 38.0 74-75 25.892522103234768 31.5 20.5 35.0 2.0 38.0 76-77 25.234908510178457 31.0 18.5 35.0 2.0 37.0 78-79 24.55566303105094 31.0 15.0 34.0 2.0 36.0 80-81 23.807678017860766 30.0 7.5 34.0 2.0 36.0 82-83 23.09772261203399 29.5 6.0 34.0 2.0 35.0 84-85 22.38508512158223 29.0 2.0 34.0 2.0 35.0 86-87 21.671028635629476 29.0 2.0 33.5 2.0 35.0 88-89 20.82948511300325 27.5 2.0 33.0 2.0 34.5 90-91 19.806945010418506 26.0 2.0 32.5 2.0 34.0 92-93 18.7053278735855 25.0 2.0 32.0 2.0 34.0 94-95 17.676788922563198 23.0 2.0 31.5 2.0 34.0 96-97 16.76594674766099 19.0 2.0 31.0 2.0 34.0 98-99 15.69370006252753 6.0 2.0 31.0 2.0 34.0 100-101 14.19560063550409 2.0 2.0 30.0 2.0 33.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 14.801119762890607 1101 2 7.964238387994808 1101 3 3.703404344985511 1101 4 2.423958730498555 1101 5 1.771741526439122 1101 6 1.432350042549416 1101 7 1.1584554985050346 1101 8 1.21262499174032 1101 9 1.1067328473415046 1101 10-11 1.061628997896463 1101 12-13 0.7318720483566565 1101 14-15 0.8343558180841271 1101 16-17 1.0073364147359953 1101 18-19 1.0262215226154225 1101 20-21 0.9960199553021383 1101 22-23 0.847096975805691 1101 24-25 1.0874443572914245 1101 26-27 1.1056097322010174 1101 28-29 1.1208914259743423 1101 30-31 0.9923514617533442 1101 32-33 1.0329653916075543 1101 34-35 1.1461544277540838 1101 36-37 1.0473165896067655 1101 38-39 0.8056881118763286 1101 40-41 1.0124927199794769 1101 42-43 0.9995904201455588 1101 44-45 0.6944010850587432 1101 46-47 0.5956336137416862 1101 48-49 0.5245283610853093 1101 50-51 0.5088173590453806 1101 52-53 0.7459886340716864 1101 54-55 0.786238647771718 1101 56-57 0.6210235731761706 1101 58-59 0.7037146713415723 1101 60-61 0.6851185628983743 1101 62-63 0.4614019317753595 1101 64-65 0.45069913832602637 1101 66-67 0.41474096816886785 1101 68-69 0.25355277921380903 1101 70-71 0.017745140546985283 1101 72-73 0.09886531117559727 1101 74-75 0.01583577176621276 1101 76-77 -0.10211242791219988 1101 78-79 -0.19172511258992486 1101 80-81 -0.4350645828680264 1101 82-83 -0.26398305535994027 1101 84-85 -0.49631558583323 1101 86-87 -0.5865131683014546 1101 88-89 -0.6604183898271785 1101 90-91 -0.566598132106428 1101 92-93 -0.515797666191947 1101 94-95 -0.6575715341338011 1101 96-97 -1.4891005651453977 1101 98-99 -1.766810131731809 1101 100-101 -1.6158283935364803 1102 1 14.868465012506213 1102 2 8.093043720761532 1102 3 3.925465529024372 1102 4 2.313406548570697 1102 5 1.8359936131072558 1102 6 1.5448792083400207 1102 7 1.2611288607644937 1102 8 1.0503298412739568 1102 9 1.2629463553754618 1102 10-11 1.1793127684925437 1102 12-13 0.912723176026482 1102 14-15 1.0614118035111275 1102 16-17 1.3989481355498654 1102 18-19 1.1920521292149644 1102 20-21 1.0379759091240643 1102 22-23 1.1205411817427304 1102 24-25 1.0206883133217062 1102 26-27 1.213498820183574 1102 28-29 1.3117042630561997 1102 30-31 1.2144183133681992 1102 32-33 1.1716949833331611 1102 34-35 1.2680405392590899 1102 36-37 1.3647198485296386 1102 38-39 1.1647848380621824 1102 40-41 1.178195972812489 1102 42-43 1.0696341027097418 1102 44-45 0.9892369453901111 1102 46-47 0.9109109760207055 1102 48-49 0.8020397751388515 1102 50-51 0.7726164619236684 1102 52-53 0.9599104010751915 1102 54-55 0.88714917163799 1102 56-57 0.8630645184122763 1102 58-59 0.8794773159258824 1102 60-61 1.1041013419131396 1102 62-63 0.5509951954056547 1102 64-65 0.5407877036469522 1102 66-67 0.5364863904343977 1102 68-69 0.2628301566063591 1102 70-71 0.3058205828735119 1102 72-73 0.5527976976607185 1102 74-75 0.4734300791195274 1102 76-77 0.36152951641753717 1102 78-79 0.3205491900870818 1102 80-81 0.36525760215810266 1102 82-83 0.326185411669659 1102 84-85 -0.06930763033459186 1102 86-87 0.0065762118168670725 1102 88-89 -0.057234723470042326 1102 90-91 0.03300362622012898 1102 92-93 0.21208756339377643 1102 94-95 0.18708987598577664 1102 96-97 -0.24317366309578148 1102 98-99 -0.7033238345970236 1102 100-101 -0.8058352124083079 1103 1 14.74218882473674 1103 2 7.829158306794056 1103 3 3.7815740228140484 1103 4 2.0851003855209385 1103 5 1.7253456437733874 1103 6 1.2295544116574604 1103 7 0.9291394628825742 1103 8 0.986875497064787 1103 9 1.2149013892664868 1103 10-11 1.1342232097592628 1103 12-13 0.6883535881796732 1103 14-15 0.9346756152697466 1103 16-17 1.015794154218149 1103 18-19 0.827183857492436 1103 20-21 0.7305078949932877 1103 22-23 0.8998439983103097 1103 24-25 1.1411098046968036 1103 26-27 1.0198839519649994 1103 28-29 1.1702433831841361 1103 30-31 1.232638232706563 1103 32-33 1.1554275683083617 1103 34-35 0.9343180619652358 1103 36-37 1.0484216428078241 1103 38-39 1.0583151886339834 1103 40-41 0.8848899256131233 1103 42-43 0.8188680552510377 1103 44-45 0.7463424009234316 1103 46-47 0.6529437635959319 1103 48-49 0.5662612615398217 1103 50-51 0.48187458787239024 1103 52-53 0.7409253058132172 1103 54-55 0.4977970878294755 1103 56-57 0.5828998918108574 1103 58-59 0.4612709372668462 1103 60-61 0.45221238526180585 1103 62-63 0.4108729190325242 1103 64-65 0.4298577608260352 1103 66-67 0.22198205901128532 1103 68-69 0.1941890094791212 1103 70-71 0.12540824899394565 1103 72-73 -0.01232340426573586 1103 74-75 0.05223837392286157 1103 76-77 0.02556830993237824 1103 78-79 -0.21508150265789538 1103 80-81 -0.18904068136416186 1103 82-83 -0.29466727700546613 1103 84-85 -0.43405553455841783 1103 86-87 -0.43997599536700704 1103 88-89 -0.596087385786543 1103 90-91 -0.6711999150607681 1103 92-93 -0.606821368204109 1103 94-95 -0.5817108364622747 1103 96-97 -1.2509647616615105 1103 98-99 -1.4850428227745454 1103 100-101 -1.444027995119944 1104 1 14.756998099792252 1104 2 7.958114410915279 1104 3 3.84225420451245 1104 4 2.2980241020988217 1104 5 1.7080802790087333 1104 6 1.3269629316030063 1104 7 1.143516458657082 1104 8 0.9882265909419843 1104 9 0.981520005246459 1104 10-11 0.8919705375143039 1104 12-13 0.74429011299015 1104 14-15 0.7793549182992692 1104 16-17 1.010402265713772 1104 18-19 0.8006031354085152 1104 20-21 0.8916967556280113 1104 22-23 1.0680143951400325 1104 24-25 1.1662497345885257 1104 26-27 1.0530000079529813 1104 28-29 1.065139798799926 1104 30-31 0.9586557700799077 1104 32-33 1.1110007837255296 1104 34-35 1.006087900251245 1104 36-37 1.0056282624602275 1104 38-39 0.9471257504753012 1104 40-41 1.2829962818001377 1104 42-43 1.191517467721873 1104 44-45 0.9182455796048785 1104 46-47 0.9685201257381983 1104 48-49 0.8524590321724901 1104 50-51 0.8142940459135062 1104 52-53 0.8136506600825406 1104 54-55 0.8635633512942356 1104 56-57 0.8306325899996452 1104 58-59 0.8704180946367117 1104 60-61 0.6556124196635054 1104 62-63 0.5247531505540728 1104 64-65 0.5636783336028444 1104 66-67 0.37879157111176553 1104 68-69 0.4474802976811567 1104 70-71 0.35232339386764977 1104 72-73 0.38716682418505144 1104 74-75 0.10471912026278929 1104 76-77 0.033112125530124104 1104 78-79 0.09542185785513624 1104 80-81 -0.014260392808221667 1104 82-83 -0.04113681063978447 1104 84-85 -0.051717091161329165 1104 86-87 0.01051339635042936 1104 88-89 0.016170263570256083 1104 90-91 0.08328742004703571 1104 92-93 0.11036827328462806 1104 94-95 0.19707241109747997 1104 96-97 -0.43649334332199174 1104 98-99 -0.6731640707549005 1104 100-101 -0.5457664879031316 1105 1 14.794262314264433 1105 2 8.005018026458597 1105 3 3.8940819471794477 1105 4 2.301447835401227 1105 5 1.6916097456441577 1105 6 1.2623004267271085 1105 7 1.1306309470782594 1105 8 1.1905572403459175 1105 9 1.0658578559243495 1105 10-11 0.9914965240168172 1105 12-13 0.9194045636061361 1105 14-15 0.9686037361933799 1105 16-17 1.2244201662698728 1105 18-19 1.1441427765734815 1105 20-21 1.2111030167647527 1105 22-23 1.0006467639305683 1105 24-25 1.1162648412582499 1105 26-27 1.0284040278989224 1105 28-29 1.239818513682053 1105 30-31 1.1097127592595086 1105 32-33 0.6658806284283472 1105 34-35 0.9463291302926891 1105 36-37 1.006156507926093 1105 38-39 1.036275391361869 1105 40-41 1.2440420311243443 1105 42-43 1.1763882484228532 1105 44-45 1.0642481780958875 1105 46-47 0.7446097963819831 1105 48-49 0.9475101878455661 1105 50-51 0.8959593361490477 1105 52-53 1.063137130906636 1105 54-55 1.0147404849842054 1105 56-57 0.9379940634819199 1105 58-59 0.8845705426620114 1105 60-61 0.8446793000391217 1105 62-63 0.7504656475658713 1105 64-65 0.8094566075510237 1105 66-67 0.7766625734046286 1105 68-69 0.6911348543222324 1105 70-71 0.6221518353144333 1105 72-73 0.7666972712900808 1105 74-75 0.511217772271177 1105 76-77 0.5795656742928195 1105 78-79 0.5297743631840746 1105 80-81 0.5808418717950872 1105 82-83 0.673944001567925 1105 84-85 0.4947430837842788 1105 86-87 0.27757491845552806 1105 88-89 0.48187560635913584 1105 90-91 0.7014474143187286 1105 92-93 0.8033145526110097 1105 94-95 0.7781646649252814 1105 96-97 0.5522544735048314 1105 98-99 0.23488017694270624 1105 100-101 0.20543636975896007 1106 1 14.707442623736231 1106 2 7.82481635960654 1106 3 3.590526814327749 1106 4 2.26020039198697 1106 5 1.7559918886673387 1106 6 1.2282126839882395 1106 7 1.0366769692017712 1106 8 1.099725672919476 1106 9 1.1341423565075957 1106 10-11 1.0937063539925873 1106 12-13 0.7590878671611634 1106 14-15 0.9502061513468973 1106 16-17 1.0637333126142252 1106 18-19 0.8690630750348518 1106 20-21 0.8607840845520798 1106 22-23 0.8038456563935696 1106 24-25 1.0011535122289743 1106 26-27 0.9631816315371395 1106 28-29 1.084577927630562 1106 30-31 0.9785137352926228 1106 32-33 0.8967644868006843 1106 34-35 0.8458922328444416 1106 36-37 0.9874481644786925 1106 38-39 1.0270838851008577 1106 40-41 0.9668121805899972 1106 42-43 0.8019004353737813 1106 44-45 0.7641951933258397 1106 46-47 0.7826464373573181 1106 48-49 0.7261045424363033 1106 50-51 0.6759505665342722 1106 52-53 0.6395161291314331 1106 54-55 0.668484306747235 1106 56-57 0.8533170593057626 1106 58-59 0.6547936767162028 1106 60-61 0.6560307777484589 1106 62-63 0.5431993420172034 1106 64-65 0.6846133860971761 1106 66-67 0.613109056114439 1106 68-69 0.6091749583676211 1106 70-71 0.7480768375607916 1106 72-73 0.5303088560157434 1106 74-75 0.5978598216260771 1106 76-77 0.5986539066416263 1106 78-79 0.41354252829244587 1106 80-81 0.4574302173441325 1106 82-83 0.582856838595788 1106 84-85 0.3244784816344577 1106 86-87 0.43472553790033075 1106 88-89 0.36165125710418167 1106 90-91 0.5425588560944234 1106 92-93 0.9418512713894529 1106 94-95 0.8000313706128352 1106 96-97 0.32018929880888436 1106 98-99 0.15176120132568371 1106 100-101 0.06169536463968761 1107 1 14.569998464135281 1107 2 7.709797356145316 1107 3 3.6431879725196055 1107 4 2.112780165074369 1107 5 1.2520821879778765 1107 6 1.0720078613996051 1107 7 0.788398510991243 1107 8 0.7819792850718557 1107 9 0.5569440711804248 1107 10-11 0.6290317768956868 1107 12-13 0.5613472871969094 1107 14-15 0.6250074604709468 1107 16-17 0.8879978842208729 1107 18-19 0.7217752120064986 1107 20-21 0.7289165648693441 1107 22-23 0.6456179576675325 1107 24-25 0.8997930652127408 1107 26-27 0.6697696947732439 1107 28-29 0.8043035489024746 1107 30-31 0.6593240795793491 1107 32-33 0.6821213043323482 1107 34-35 0.7479755577780125 1107 36-37 0.8890317813306794 1107 38-39 0.8388820110996775 1107 40-41 1.0145077263486186 1107 42-43 0.6477839824937917 1107 44-45 0.5412790714145714 1107 46-47 0.49897081816526523 1107 48-49 0.5690382728744083 1107 50-51 0.546516044518846 1107 52-53 0.67917071642999 1107 54-55 0.5712985832050386 1107 56-57 0.585632639587164 1107 58-59 0.7922229380047696 1107 60-61 0.7155030706734742 1107 62-63 0.46586426756448773 1107 64-65 0.1735334669276476 1107 66-67 0.12798091538547496 1107 68-69 0.3099046904920293 1107 70-71 0.17292266952926383 1107 72-73 0.16916978120389103 1107 74-75 0.09916256349574937 1107 76-77 0.06929513492340433 1107 78-79 0.21197431128018707 1107 80-81 0.13357812739608477 1107 82-83 0.3098091739283788 1107 84-85 0.3169310359181523 1107 86-87 0.3018243310190236 1107 88-89 0.4739187438631234 1107 90-91 0.5665943083742455 1107 92-93 0.8438435838602629 1107 94-95 1.002307921249347 1107 96-97 0.6040542691592776 1107 98-99 0.2705354645549676 1107 100-101 0.1851635304863244 1108 1 14.19425077035774 1108 2 7.474081821485694 1108 3 3.376270246072483 1108 4 -0.06439497526464066 1108 5 0.4448651859299275 1108 6 0.6418395650633499 1108 7 0.5493076403408423 1108 8 0.5642926844757525 1108 9 0.7136010123995575 1108 10-11 0.6473167039376833 1108 12-13 0.30420648305896947 1108 14-15 0.20281080262690665 1108 16-17 0.6350710121198446 1108 18-19 0.43162336093346454 1108 20-21 0.4604266434500417 1108 22-23 -0.3544417549803498 1108 24-25 0.3489854587928036 1108 26-27 0.3319128188475631 1108 28-29 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0.39034823867574886 2312 64-65 0.3680643754460391 2312 66-67 0.33137578047460536 2312 68-69 0.4142890872860683 2312 70-71 0.5400948444309748 2312 72-73 0.2984002308627467 2312 74-75 0.5055636870281681 2312 76-77 0.5842490361102399 2312 78-79 0.7812610274746632 2312 80-81 0.8732813128420602 2312 82-83 0.7596256099297136 2312 84-85 0.7013385646936712 2312 86-87 0.7558995215193143 2312 88-89 1.1017494113729107 2312 90-91 1.412241271691542 2312 92-93 1.893566379927055 2312 94-95 1.9123488545438754 2312 96-97 1.669278254488102 2312 98-99 1.5627283474061429 2312 100-101 1.529352955100764 2313 1 -14.098947759054024 2313 2 -7.376763809724125 2313 3 -3.6927856967312884 2313 4 -1.219826310569072 2313 5 -0.5693985960001413 2313 6 -1.1075263816367809 2313 7 -0.40688036890728085 2313 8 -0.14313890008092045 2313 9 -2.7818008925178717 2313 10-11 -0.5906337765174996 2313 12-13 -0.8351177317964655 2313 14-15 -1.0407549261923847 2313 16-17 -0.8275033696199969 2313 18-19 -0.23133424613040177 2313 20-21 -0.9977486689747437 2313 22-23 -1.922402205525266 2313 24-25 -1.3013950269052756 2313 26-27 -0.5310261122041098 2313 28-29 -0.04614261242595319 2313 30-31 -1.394941350257433 2313 32-33 -0.3806284214722808 2313 34-35 -1.2039739862773757 2313 36-37 -1.3682963703445523 2313 38-39 -0.8056176307249601 2313 40-41 -0.4649635930066438 2313 42-43 -0.11201465264278454 2313 44-45 -0.09205336564767208 2313 46-47 -0.561362109605394 2313 48-49 -0.179974042435461 2313 50-51 -0.9082134236211701 2313 52-53 -0.023554450335481647 2313 54-55 -0.775258018948886 2313 56-57 -0.34305798828162537 2313 58-59 -0.14324009290186623 2313 60-61 -1.4224052996567842 2313 62-63 -0.1930445520519335 2313 64-65 -0.09901414005578246 2313 66-67 -1.280332731777186 2313 68-69 -0.2278871706518366 2313 70-71 0.17512336327942535 2313 72-73 -0.6792137151508584 2313 74-75 -0.13775672323475519 2313 76-77 0.3606915046984547 2313 78-79 0.49100541013095267 2313 80-81 0.4322115834640563 2313 82-83 0.47177715452241387 2313 84-85 0.4703747062790917 2313 86-87 0.5254579278863396 2313 88-89 0.7580930610816843 2313 90-91 1.2500505852725965 2313 92-93 1.6830589990448281 2313 94-95 1.8660504913587914 2313 96-97 1.3130847030737876 2313 98-99 1.366648412517165 2313 100-101 1.0806297825186046 2314 1 -14.098947759054024 2314 2 -7.187749201173817 2314 3 -3.4908029808032417 2314 4 -1.3801406608376183 2314 5 -1.3307380227059404 2314 6 -6.226146750661506 2314 7 -2.1315623261609673 2314 8 -4.321539421175093 2314 9 -1.519738130502681 2314 10-11 -0.52749815715881 2314 12-13 -3.110033592443507 2314 14-15 -2.964760457764555 2314 16-17 -2.5733965989854184 2314 18-19 -1.173802776426669 2314 20-21 -2.1226553986243815 2314 22-23 -1.2340252856197935 2314 24-25 -2.7652276515407834 2314 26-27 -4.6429517524281785 2314 28-29 -1.7491841929667586 2314 30-31 -1.8264488500474556 2314 32-33 -3.9187163557653477 2314 34-35 -2.5123692413650325 2314 36-37 -0.9005686085924864 2314 38-39 -1.3901469678877376 2314 40-41 -2.4521318406950527 2314 42-43 -1.3869757326376373 2314 44-45 -2.326317119735272 2314 46-47 -2.131492874549476 2314 48-49 -1.1512192421272367 2314 50-51 -2.1191551465985583 2314 52-53 -0.8723046189278456 2314 54-55 -0.32159826639891165 2314 56-57 -0.9335162005508266 2314 58-59 -1.4132821965582032 2314 60-61 -0.5024621906497515 2314 62-63 -2.90620211020018 2314 64-65 -1.141523376477867 2314 66-67 -2.271108856100941 2314 68-69 -2.2276229119197986 2314 70-71 -2.7044095060458915 2314 72-73 -2.6691325886785293 2314 74-75 -2.170511911087601 2314 76-77 -1.061095664995559 2314 78-79 -0.6585855072450215 2314 80-81 -0.2825503466893906 2314 82-83 -0.39555703336075254 2314 84-85 -0.226551022815368 2314 86-87 -0.08933866172098348 2314 88-89 0.3119438152776759 2314 90-91 0.6088770883062153 2314 92-93 0.9865467786685542 2314 94-95 0.9716610187714743 2314 96-97 0.859914608972236 2314 98-99 0.9986214711956727 2314 100-101 1.1845499427501824 2315 1 -14.098947759054024 2315 2 -7.376433451349419 2315 3 -4.7379042270975695 2315 4 -3.1899030521608793 2315 5 -3.64619287875502 2315 6 -3.020933662555098 2315 7 -3.4332443884778066 2315 8 -3.8917859213437964 2315 9 -2.65328611166715 2315 10-11 -4.852296241774404 2315 12-13 -4.446159310500448 2315 14-15 -3.5662404455414034 2315 16-17 -4.8416610285611945 2315 18-19 -1.7130738862928325 2315 20-21 -3.434528458252558 2315 22-23 -4.277646913378227 2315 24-25 -5.188169027111883 2315 26-27 -4.63399314985665 2315 28-29 -2.6873098070420482 2315 30-31 -3.261780753102606 2315 32-33 -4.429462010287381 2315 34-35 -3.838541899708922 2315 36-37 -5.131672331347705 2315 38-39 -3.801324358457663 2315 40-41 -4.927950802279138 2315 42-43 -3.4844850538496814 2315 44-45 -2.100322291230718 2315 46-47 -1.5384263814685006 2315 48-49 -1.1608189764435153 2315 50-51 -0.9621826465614234 2315 52-53 -0.5709883491952077 2315 54-55 -2.0888974173569217 2315 56-57 -4.208148710307061 2315 58-59 -3.702133666200865 2315 60-61 -4.536229965675904 2315 62-63 -2.611030572311602 2315 64-65 -3.036683349060663 2315 66-67 -1.8192656222292385 2315 68-69 -1.3249001312383157 2315 70-71 -1.9804208336793323 2315 72-73 -2.1874667814520166 2315 74-75 -2.750122595308067 2315 76-77 -2.3870786629350214 2315 78-79 -1.6215277210355339 2315 80-81 -1.391769540004006 2315 82-83 -1.0300673752906064 2315 84-85 -0.8319918625626705 2315 86-87 -1.472703348704389 2315 88-89 -2.216181363772357 2315 90-91 -1.9370952674298572 2315 92-93 -0.9202925611965256 2315 94-95 -0.6047824693247499 2315 96-97 -1.4099790432664463 2315 98-99 -0.8198621156194541 2315 100-101 -0.4522822138928646 2316 1 -14.098947759054024 2316 2 -7.382068089546335 2316 3 -5.288912530774912 2316 4 -3.156688342789586 2316 5 -2.3060386676043656 2316 6 -1.3219419662324405 2316 7 -5.0907176980863476 2316 8 -3.0371002748432048 2316 9 -1.5420761472543347 2316 10-11 -4.489603477836088 2316 12-13 -3.7787512440710316 2316 14-15 -1.952954561792886 2316 16-17 -3.3370643500321293 2316 18-19 -2.4581806478586685 2316 20-21 -3.362019561455405 2316 22-23 -3.2319766879926988 2316 24-25 -3.6322674850003764 2316 26-27 -4.1629077406039805 2316 28-29 -3.8665802696755023 2316 30-31 -2.8377000888084183 2316 32-33 -3.125932947416679 2316 34-35 -4.405564438261713 2316 36-37 -2.7402132566146093 2316 38-39 -3.465766825836077 2316 40-41 -2.9301750015673917 2316 42-43 -6.793945314822398 2316 44-45 -3.5980980919424645 2316 46-47 -4.937744293686766 2316 48-49 -4.573926924248973 2316 50-51 -3.4469098114487267 2316 52-53 -4.778728562718339 2316 54-55 -4.775137037760242 2316 56-57 -4.9582969902596155 2316 58-59 -3.5596366317999184 2316 60-61 -3.3141065967553835 2316 62-63 -2.3214398249806436 2316 64-65 -2.407234950484149 2316 66-67 -2.6407365593585403 2316 68-69 -2.8057720173593133 2316 70-71 -2.8853733840945175 2316 72-73 -3.8502781693523715 2316 74-75 -4.481439321286718 2316 76-77 -4.263561462460526 2316 78-79 -4.5411599867532075 2316 80-81 -4.232072031107208 2316 82-83 -3.8408621558362768 2316 84-85 -3.439643108114275 2316 86-87 -3.596220549178888 2316 88-89 -3.1226167137130467 2316 90-91 -2.3579137727679367 2316 92-93 -2.126698255146703 2316 94-95 -1.285535731483705 2316 96-97 -1.5711593516887472 2316 98-99 -1.882139695690629 2316 100-101 -1.8088954997766127 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 22836.0 3 7173.0 4 8029.0 5 9338.0 6 10592.0 7 13169.0 8 16442.0 9 20009.0 10 23626.0 11 27659.0 12 31878.0 13 35202.0 14 37962.0 15 41871.0 16 46653.0 17 48629.0 18 45699.0 19 46089.0 20 49576.0 21 54386.0 22 59534.0 23 65942.0 24 72930.0 25 81324.0 26 91274.0 27 102380.0 28 113856.0 29 128705.0 30 146172.0 31 166159.0 32 189234.0 33 215598.0 34 246863.0 35 284212.0 36 289280.0 37 161803.0 38 18488.0 39 93.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.462019276482955 18.753656764224335 13.394566855193176 23.38975710409953 2 15.166608158996436 20.648342042334892 40.714403421559375 23.47064637710929 3 19.019803913212815 25.78033509639001 30.04870291289205 25.15115807750512 4 12.20978358893799 16.624484625373228 38.43061649251698 32.735115293171795 5 13.218392703175017 38.23690895816747 34.47442576484852 14.070272573808998 6 30.917726099079207 36.74436755569331 18.423748292472737 13.914158052754743 7 27.59593368024541 33.264367291921324 21.724269999243255 17.415429028590008 8 25.869735325529508 34.8787988135647 20.66632564582474 18.585140215081054 9 27.16632826865223 16.20708967244504 20.33451015441853 36.2920719044842 10-11 24.582971097503947 26.596107559552728 28.27720667074452 20.543714672198806 12-13 26.102546082562945 24.103356575957818 28.288581905842342 21.505515435636894 14-15 22.938282337986035 25.125123686290095 26.809884197084532 25.126709778639334 16-17 22.187541456804716 28.43097135348217 27.25897147970085 22.122515710012262 18-19 21.953125980989213 27.309946660621847 29.501596348258357 21.235331010130583 20-21 23.034927587656114 26.397985203029073 29.199698620008185 21.36738858930663 22-23 22.53146981451591 26.574813073723373 28.953931877593558 21.939785234167157 24-25 22.405719220328173 26.51918236669628 29.27196801315822 21.803130399817334 26-27 22.36079977132197 26.69523378575719 29.15826744980185 21.78569899311899 28-29 22.10301929857365 26.886935550239894 29.27837291067683 21.731672240509628 30-31 22.19661373873185 26.772873070676162 29.1937593637735 21.83675382681849 32-33 21.98418902278532 26.82215097538942 29.161822455185256 22.031837546640002 34-35 22.536081823211752 26.775442919495468 28.927896195834286 21.760579061458497 36-37 22.45563630972245 26.608660524898227 29.047632506778044 21.88807065860128 38-39 22.319972021009367 26.809866164296576 29.165982122403456 21.704179692290605 40-41 22.4567546853464 26.612787393724464 29.187994498687893 21.74246342224124 42-43 22.18911601750648 26.832933342875148 28.800304632909246 22.177646006709132 44-45 22.35950008033573 27.032907234381316 28.544574428905523 22.063018256377433 46-47 22.415322768650913 27.058223253786828 28.093621873078973 22.432832104483282 48-49 22.63022077292266 27.098473721552274 28.022551004228024 22.248754501297043 50-51 22.518080688752864 27.29464676677466 27.956938394030033 22.230334150442445 52-53 22.601885611818165 27.35915266396137 27.890160192150525 22.14880153206994 54-55 22.149456586904336 27.20427751238112 28.081660397204665 22.56460550350988 56-57 22.48217747327861 27.629496471870535 27.665359780580705 22.22296627427015 58-59 22.596050469292493 27.21208906847395 27.774671510130332 22.417188952103224 60-61 22.47918248321896 27.15517932478467 28.121205990314113 22.24443220168226 62-63 22.54647995724636 27.153770127760062 28.019951259128167 22.27979865586541 64-65 22.370367260600304 27.178814943637654 27.966669680327698 22.484148115434348 66-67 22.182591409925013 27.44916050743455 27.757075279111813 22.611172803528625 68-69 22.237684961309085 27.303212174692298 27.951633507079794 22.507469356918822 70-71 22.141576188516996 27.309901664881707 27.844150185325724 22.704371961275577 72-73 22.305437806578354 27.164629796278795 27.715253571474037 22.814678825668818 74-75 22.20180463806437 27.41638139290115 27.542916756732083 22.838897212302392 76-77 22.35153853761445 27.127539408188355 27.653042559884206 22.86787949431299 78-79 22.197002696587443 27.229149736509704 27.666299205775125 22.90754836112773 80-81 22.118355126239205 27.451387685277183 27.582816570458586 22.847440618025026 82-83 22.235773137503408 27.383336817066944 27.47238700135204 22.90850304407761 84-85 22.160398707938697 27.19204144726869 27.360566869660946 23.28699297513167 86-87 22.20718200438211 27.096878622961967 27.61233538153654 23.083603991119382 88-89 22.293098416776825 26.925607276990064 27.481219324069272 23.300074982163835 90-91 21.936235063953234 27.084003912794998 27.692480896854672 23.2872801263971 92-93 21.955638758530643 27.04343198605902 27.787877821015915 23.21305143439442 94-95 21.914208797820407 26.950746481430826 27.526140990177346 23.608903730571424 96-97 22.00285790287233 26.803575587858514 27.350689628704224 23.842876880564937 98-99 21.908022497427567 26.825798148907715 27.247562869681136 24.01861648398358 100-101 21.993154040378553 26.766778912961943 27.43516084729215 23.804906199367355 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3562.0 1 2422.0 2 1182.5 3 958.5 4 813.5 5 811.5 6 1039.0 7 2148.0 8 2965.5 9 2732.0 10 2432.0 11 2313.0 12 2505.0 13 2751.0 14 2989.5 15 3271.5 16 3517.5 17 3843.5 18 4210.5 19 4673.5 20 5398.5 21 6366.5 22 7583.5 23 9120.5 24 11353.0 25 14457.0 26 18489.0 27 23253.5 28 28390.0 29 33612.5 30 38901.5 31 44592.5 32 50109.0 33 55336.0 34 60753.0 35 66255.0 36 72847.0 37 79278.0 38 83171.0 39 86002.0 40 89805.0 41 94016.0 42 97645.0 43 102079.5 44 106236.0 45 109173.0 46 113202.5 47 122139.5 48 129924.5 49 125949.5 50 118216.5 51 113262.0 52 108613.0 53 104123.0 54 97921.0 55 89898.5 56 80895.5 57 71909.5 58 63629.5 59 54956.5 60 45994.5 61 37444.5 62 29355.0 63 22060.0 64 16401.0 65 12052.0 66 8641.5 67 6278.0 68 4319.5 69 2881.0 70 2036.0 71 1411.5 72 988.5 73 749.5 74 558.0 75 391.5 76 265.5 77 172.0 78 107.0 79 72.0 80 49.5 81 34.5 82 27.5 83 24.5 84 22.0 85 19.5 86 13.0 87 8.5 88 9.5 89 8.5 90 6.5 91 3.0 92 3.0 93 5.0 94 6.5 95 6.5 96 6.0 97 5.0 98 2.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 52.45851982980633 2 0.0848658627726918 3 0.11667406328314082 4 0.187450609024752 5 0.1186208307417679 6 0.14382981952805737 7 0.14973611402118017 8 0.14706343327289553 9 0.13693364327631063 10-11 0.12361973362281875 12-13 0.13572928713665153 14-15 0.14384631755736776 16-17 0.13764305853665781 18-19 0.1446217249349565 20-21 0.17317981367125698 22-23 0.18947986662993105 24-25 0.21600869776105244 26-27 0.20871656880585612 28-29 0.20690178558171227 30-31 0.22142005137486326 32-33 0.2123956293420751 34-35 0.19951066845065357 36-37 0.18611426865060968 38-39 0.1827321726419779 40-41 0.18373855242991224 42-43 0.17766727764368548 44-45 0.19332390745925399 46-47 0.2165861287869164 48-49 0.22026518932313532 50-51 0.23087342216972181 52-53 0.2582436527956736 54-55 0.24458328452666328 56-57 0.22051265976279133 58-59 0.17882213969541338 60-61 0.18967784298165585 62-63 0.19446227148167153 64-65 0.18317761943335867 66-67 0.19144313211786854 68-69 0.18053793474369487 70-71 0.1932084212540812 72-73 0.21310504460242224 74-75 0.24415433576459294 76-77 0.24324694415252096 78-79 0.23803356689043492 80-81 0.24275200327320903 82-83 0.2339090595628352 84-85 0.20442708118515243 86-87 0.21615718002484605 88-89 0.2181864376300251 90-91 0.20551595111963877 92-93 0.20459206147825643 94-95 0.24245503874562183 96-97 0.2695448028732968 98-99 0.28770913314404595 100-101 0.28345264158196304 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3030665.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 71.56420315859583 #Duplication Level Percentage of deduplicated Percentage of total 1 82.81643793241787 59.26692389066793 2 10.680049881436412 15.286185189181053 3 3.1648481205892405 6.794695016038458 4 1.327666872778505 3.8005368724183413 5 0.6620646953499602 2.36900661810792 6 0.3829983098570987 1.6445381313607348 7 0.22401297955610713 1.1221917265380936 8 0.1528114318241405 0.8748662681614965 9 0.11508453034453053 0.7412339438988812 >10 0.4454551108810303 5.310644496826804 >50 0.018962022390516278 0.9300502945364455 >100 0.009004352705502079 1.2348138264006947 >500 3.018799345914562E-4 0.12634620593444795 >1k 3.0187993447165795E-4 0.49796751992876814 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4740 0.1564013178625813 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3041 0.10034101426584593 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 3.2996058620797744E-5 0.0 5 3.2996058620797744E-5 0.0 0.0 3.2996058620797744E-5 0.0 6 3.2996058620797744E-5 0.0 0.0 3.2996058620797744E-5 0.0 7 3.2996058620797744E-5 0.0 0.0 3.2996058620797744E-5 0.0 8 3.2996058620797744E-5 0.0 0.0 9.898817586239323E-5 0.0 9 3.2996058620797744E-5 0.0 0.0 1.3198423448319098E-4 0.0 10-11 6.599211724159549E-5 0.0 0.0 3.9595270344957293E-4 0.0 12-13 8.249014655199437E-5 0.0 0.0 3.9595270344957293E-4 0.0 14-15 1.6498029310398873E-4 0.0 0.0 3.9595270344957293E-4 0.0 16-17 1.6498029310398873E-4 0.0 0.0 4.6194482069116845E-4 0.0 18-19 1.6498029310398873E-4 0.0 0.0 5.444349672431628E-4 0.0 20-21 1.6498029310398873E-4 9.898817586239323E-5 0.0 7.424113189679493E-4 0.0 22-23 1.9797635172478647E-4 9.898817586239323E-5 0.0 9.568857000031347E-4 0.0 24-25 4.6194482069116845E-4 1.154862051727921E-4 0.0 0.0012373521982799154 0.0 26-27 4.6194482069116845E-4 1.3198423448319098E-4 0.0 0.0017322930775918815 0.0 28-29 6.764192017263538E-4 1.3198423448319098E-4 0.0 0.002969645275871797 0.0 30-31 7.589093482783481E-4 1.4848226379358986E-4 0.0 0.007770571805197869 0.0 32-33 7.589093482783481E-4 1.8147832241438761E-4 0.0 0.017306432746608418 0.0 34-35 7.919054068991459E-4 1.9797635172478647E-4 0.0 0.032946564532866554 0.0 36-37 9.238896413823369E-4 1.9797635172478647E-4 0.0 0.058221545436397626 0.0 38-39 0.0011218659931071233 1.9797635172478647E-4 0.0 0.09626600102617743 0.0 40-41 0.0011218659931071233 1.9797635172478647E-4 0.0 0.14015075899183843 0.0 42-43 0.0012043561396591178 1.9797635172478647E-4 0.0 0.18599878244543688 0.0 44-45 0.0012538502275903144 1.9797635172478647E-4 0.0 0.24134967078182512 0.0 46-47 0.0013198423448319099 1.9797635172478647E-4 0.0 0.30773774072687016 0.0 48-49 0.001451826579315101 1.9797635172478647E-4 0.0 0.38115397115814514 0.0 50-51 0.001451826579315101 1.9797635172478647E-4 0.0 0.4603775079066805 0.0 52-53 0.001451826579315101 1.9797635172478647E-4 0.0 0.5395350525379743 0.0 54-55 0.001451826579315101 1.9797635172478647E-4 0.0 0.6250278404244614 0.0 56-57 0.0015838108137982917 1.9797635172478647E-4 0.0 0.7282230137610063 0.0 58-59 0.0015838108137982917 2.1447438103518534E-4 0.0 0.8424553687062081 0.0 60-61 0.0015838108137982917 2.3097241034558422E-4 0.0 0.9773597543773396 0.0 62-63 0.0015838108137982917 2.6396846896638195E-4 0.0 1.116553627669175 0.0 64-65 0.0015838108137982917 2.6396846896638195E-4 0.0 1.25449365073342 0.0 66-67 0.0015838108137982917 2.6396846896638195E-4 0.0 1.4111919331235883 0.0 68-69 0.0015838108137982917 2.6396846896638195E-4 0.0 1.5909544604896946 0.0 70-71 0.0016168068724190894 2.6396846896638195E-4 0.0 1.797278815045543 0.0 72-73 0.0016663009603502862 2.6396846896638195E-4 0.0 2.012413117253144 0.0 74-75 0.001682798989660685 2.6396846896638195E-4 0.0 2.235845928203876 0.0 76-77 0.001682798989660685 2.6396846896638195E-4 0.0 2.4677422281908425 0.0 78-79 0.001682798989660685 2.6396846896638195E-4 0.0 2.7312487523365334 0.0 80-81 0.0019137714000062693 2.6396846896638195E-4 0.0 3.0350434640582185 0.0 82-83 0.001946767458627067 2.6396846896638195E-4 0.0 3.3471696805816546 0.0 84-85 0.0019797635172478646 2.6396846896638195E-4 0.0 3.6637338669895883 0.0 86-87 0.0020127595758686625 2.6396846896638195E-4 0.0 3.9952287699234326 0.0 88-89 0.0020127595758686625 2.6396846896638195E-4 0.0 4.354654836479782 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 10875 0.0 34.07004 1 GTATCAA 14995 0.0 31.995857 1 TACATGG 12995 0.0 28.3965 2 ACATGGG 12760 0.0 28.179367 3 GGTATCA 11095 0.0 26.321651 1 TATCAAC 19410 0.0 25.87604 2 ATCAACG 20740 0.0 24.308207 3 CATGGGA 8830 0.0 24.129908 4 TCAACGC 21035 0.0 23.896751 4 CAACGCA 21160 0.0 23.804457 5 AACGCAG 21520 0.0 23.66948 6 ACGCAGA 22420 0.0 22.382425 7 GAGTACT 8945 0.0 22.114233 12-13 AGTACTT 9595 0.0 21.30498 12-13 CGCAGAG 23360 0.0 21.098497 8 CATGGGG 4965 0.0 20.838413 4 GTACTTT 9945 0.0 19.939701 14-15 GCAGAGT 23445 0.0 19.351034 9 ATGGGAG 4175 0.0 18.769865 5 ATGGGAT 3875 0.0 18.639284 5 >>END_MODULE