FastQCFastQC Report
Fri 27 May 2016
SRR939642_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939642_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3030665
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT112090.3698528210805219No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83360.27505514466297004No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64090.21147173970069277No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA34170.11274753230726589No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA171550.026.8195931
GTACATG128350.025.0006521
GGTATCA122100.024.889061
TACATGG127850.024.6116942
ACATGGG123650.024.1866473
TCAACGC202250.022.4604424
ATCAACG203150.022.4073773
CAACGCA204550.022.2078915
AACGCAG205350.022.1749116
TATCAAC207350.022.0918242
CATGGGA87400.021.912664
AGTACTT101050.020.52695512-13
GAGTACT98500.020.29260412-13
ACGCAGA227200.019.8556047
CGCAGAG225950.019.7359628
CATGGGG44100.018.611924
GCAGAGT237950.018.186279
GTACTTT109600.018.17290314-15
ATGGGAG40400.018.0980115
AGAGTAC197250.017.44586610-11
Produced by FastQC (version 0.11.5)