##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939642_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3030665 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.45418216794004 31.0 31.0 34.0 30.0 34.0 2 31.648953942451573 31.0 31.0 34.0 30.0 34.0 3 31.701854213514196 31.0 31.0 34.0 30.0 34.0 4 35.50877744653401 37.0 35.0 37.0 33.0 37.0 5 35.259504102234985 37.0 35.0 37.0 33.0 37.0 6 35.264295459907316 37.0 35.0 37.0 32.0 37.0 7 35.168772530121274 37.0 35.0 37.0 32.0 37.0 8 35.128051104295594 37.0 35.0 37.0 32.0 37.0 9 36.7261155554969 39.0 35.0 39.0 32.0 39.0 10-11 36.60024417083379 39.0 35.0 39.0 32.0 39.0 12-13 36.53142907579689 38.0 35.0 39.0 32.0 39.0 14-15 37.5855523457723 39.0 36.0 41.0 32.0 41.0 16-17 37.54432096586063 39.0 36.0 41.0 32.0 41.0 18-19 37.50914568254822 39.0 36.0 41.0 32.0 41.0 20-21 37.45728775697743 39.0 36.0 41.0 32.0 41.0 22-23 37.32668440754752 39.0 36.0 41.0 31.5 41.0 24-25 37.19281477827474 39.0 36.0 40.5 31.0 41.0 26-27 36.90553541879422 39.0 36.0 40.0 30.5 41.0 28-29 36.70297179001968 39.0 35.5 40.0 30.0 41.0 30-31 36.45404275959237 38.0 35.0 40.0 30.0 41.0 32-33 36.287574344244575 38.0 35.0 40.0 30.0 41.0 34-35 36.25242281809438 38.0 35.0 40.0 30.0 41.0 36-37 36.166870637302374 38.0 35.0 40.0 30.0 41.0 38-39 36.21014480320326 38.0 35.0 40.0 30.0 41.0 40-41 36.09277914253143 38.0 35.0 40.0 30.0 41.0 42-43 35.90983200056753 38.0 35.0 40.0 29.0 41.0 44-45 35.693750216536635 38.0 35.0 40.0 28.0 41.0 46-47 35.39582946317063 38.0 34.0 40.0 27.0 41.0 48-49 35.10307061321525 38.0 34.0 40.0 26.0 41.0 50-51 34.86373997126043 38.0 34.0 40.0 26.0 41.0 52-53 34.62484537222029 38.0 33.0 40.0 25.5 41.0 54-55 34.341716421973395 38.0 33.0 40.0 24.0 41.0 56-57 33.477815594927186 37.0 32.0 40.0 21.0 41.0 58-59 30.320473064492447 35.5 26.0 39.0 8.0 40.5 60-61 30.025742865014774 34.5 25.5 38.5 10.5 40.0 62-63 31.91021244512343 35.5 30.5 39.0 17.5 40.0 64-65 31.97942365784407 35.5 31.0 39.0 15.0 40.0 66-67 27.013088711553408 31.5 18.5 37.0 2.0 39.5 68-69 19.585148968955657 19.5 8.0 32.0 2.0 37.0 70-71 17.65876004111309 15.5 7.5 29.0 2.0 35.0 72-73 19.337759369643294 20.0 7.5 30.5 2.0 35.5 74-75 20.737193817198538 22.5 8.5 32.0 2.0 36.0 76-77 22.545910386004394 27.0 11.0 33.0 2.0 35.5 78-79 24.698950230394978 30.5 13.0 34.5 2.0 36.5 80-81 26.311787182021106 31.5 22.0 35.0 2.0 36.5 82-83 27.514361534514702 33.0 26.0 35.0 2.0 36.0 84-85 27.586272484751696 33.0 27.0 35.0 2.0 36.0 86-87 27.30840162142632 33.0 26.0 35.0 2.0 36.0 88-89 26.867605129567274 33.0 25.5 35.0 2.0 35.0 90-91 24.97413686435155 32.0 15.0 34.5 2.0 35.0 92-93 23.266891259839014 31.0 2.0 34.0 2.0 35.0 94-95 19.550266030722632 26.5 2.0 34.0 2.0 35.0 96-97 16.888591447751566 16.5 2.0 33.0 2.0 35.0 98-99 17.132394870432726 19.5 2.0 33.0 2.0 35.0 100-101 16.364027036970434 9.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.15257054357786615 1101 2 0.15595919545454606 1101 3 0.21840921678476732 1101 4 0.20381438932881935 1101 5 0.37728348185577687 1101 6 0.31296471858557595 1101 7 0.25634905110343453 1101 8 0.37736485505140394 1101 9 0.31269372583340527 1101 10-11 0.33011603581542204 1101 12-13 0.34036644486496925 1101 14-15 0.38170169549174204 1101 16-17 0.4256794988879662 1101 18-19 0.414814307941775 1101 20-21 0.42690875032383957 1101 22-23 0.40004662394372303 1101 24-25 0.40369349318797276 1101 26-27 0.4308807632561553 1101 28-29 0.3391000512425961 1101 30-31 0.23230411896708603 1101 32-33 0.35659574186504983 1101 34-35 0.2178801559245187 1101 36-37 0.2834672363028474 1101 38-39 0.1803120878276374 1101 40-41 0.23444227387518168 1101 42-43 0.23550943242145905 1101 44-45 0.14488457175478686 1101 46-47 0.16392905379741052 1101 48-49 0.2273645983506114 1101 50-51 0.2419818173496111 1101 52-53 0.028072211149165582 1101 54-55 0.07902612993527214 1101 56-57 -3.4974282302268307 1101 58-59 -10.428157522227394 1101 60-61 -11.825009364383497 1101 62-63 -4.014680444558312 1101 64-65 -1.1126969817308172 1101 66-67 -2.1983085282858923 1101 68-69 -4.474960137247603 1101 70-71 -4.356298646487655 1101 72-73 -3.533153855507104 1101 74-75 -6.2026369105748635 1101 76-77 -4.911816792493749 1101 78-79 1.3135685862569417 1101 80-81 1.907020464474023 1101 82-83 0.637486569550962 1101 84-85 0.35081259893879135 1101 86-87 0.24223923329645558 1101 88-89 0.16837433387037137 1101 90-91 1.5062086271883395 1101 92-93 0.688490569342985 1101 94-95 -8.731393440440137 1101 96-97 -9.547331573212498 1101 98-99 -8.685776422027775 1101 100-101 -9.640315898040257 1102 1 0.2952483243240209 1102 2 0.36697305145544235 1102 3 0.3838699862682269 1102 4 0.26151542491719937 1102 5 0.3659963375628479 1102 6 0.3418282384658724 1102 7 0.4164756314125384 1102 8 0.34351120119850265 1102 9 0.40460536143360315 1102 10-11 0.38796942918209254 1102 12-13 0.36481492304302776 1102 14-15 0.4513156424405622 1102 16-17 0.5622487439979622 1102 18-19 0.41829242154133794 1102 20-21 0.47237271143048787 1102 22-23 0.4449350482642487 1102 24-25 0.3835363702006802 1102 26-27 0.26847026094889515 1102 28-29 0.33333001436112397 1102 30-31 0.34113569149939593 1102 32-33 0.16550052614377364 1102 34-35 0.28254408644848894 1102 36-37 0.08113332850697219 1102 38-39 0.24978412311862996 1102 40-41 0.07621399813849195 1102 42-43 0.1008024861069643 1102 44-45 2.3191859577309515E-4 1102 46-47 -0.09480790355441826 1102 48-49 -0.009935030612084006 1102 50-51 -0.015570172102918889 1102 52-53 -0.14693631244061578 1102 54-55 -0.3361879332988664 1102 56-57 -3.9621529038300167 1102 58-59 -10.10011344493321 1102 60-61 -8.36106392691553 1102 62-63 -2.6703573109355396 1102 64-65 -0.9894886580842197 1102 66-67 -1.8194258497761417 1102 68-69 -4.942435585530637 1102 70-71 -4.758687874270425 1102 72-73 -3.576770075709545 1102 74-75 -3.9425011732410056 1102 76-77 -4.111363475317081 1102 78-79 1.5774030281385052 1102 80-81 1.9696810735761936 1102 82-83 0.6141858310769237 1102 84-85 0.30755754779503164 1102 86-87 0.315151376089144 1102 88-89 0.2690165924727488 1102 90-91 1.5718287111218068 1102 92-93 1.4150184700417192 1102 94-95 -8.680383256139457 1102 96-97 -9.656389182011065 1102 98-99 -8.60550698768742 1102 100-101 -9.478347133274 1103 1 0.19730584861593314 1103 2 0.2361008977880772 1103 3 0.25176248475313656 1103 4 0.17154175590539467 1103 5 0.14404759785573162 1103 6 0.280194446031949 1103 7 0.24938439252966305 1103 8 0.3977776524330281 1103 9 0.40133519172021437 1103 10-11 0.3458694483771225 1103 12-13 0.3832498564651843 1103 14-15 0.5529891386120838 1103 16-17 0.4677581849549597 1103 18-19 0.46577822349073017 1103 20-21 0.424606583407936 1103 22-23 0.46163674284731115 1103 24-25 0.4090869093514158 1103 26-27 0.42565746009657346 1103 28-29 0.47009071612118447 1103 30-31 0.38287255039765 1103 32-33 0.3169844846901597 1103 34-35 0.2925645611460155 1103 36-37 0.3701610418348551 1103 38-39 0.4379917006698477 1103 40-41 0.335209728254263 1103 42-43 0.26198089082787845 1103 44-45 0.22652600472001438 1103 46-47 0.21813563606418285 1103 48-49 0.19020343718246124 1103 50-51 0.05468767261631058 1103 52-53 0.07108392970583566 1103 54-55 0.01646455186217821 1103 56-57 0.5102106121806145 1103 58-59 -8.402813736982498 1103 60-61 -7.674985392675996 1103 62-63 -2.2511794895054287 1103 64-65 -0.4673528890724796 1103 66-67 -1.8475715972269562 1103 68-69 -3.4411400805047503 1103 70-71 -4.050981505921008 1103 72-73 -3.1686874805416334 1103 74-75 -3.8618509634242457 1103 76-77 -0.6450521859404326 1103 78-79 3.1700210090127747 1103 80-81 2.6140480446920336 1103 82-83 1.141047605399077 1103 84-85 0.6604556570527933 1103 86-87 0.4841391072133412 1103 88-89 0.4496096104563456 1103 90-91 1.7888750659475505 1103 92-93 1.2294380393630924 1103 94-95 -7.465092406811504 1103 96-97 -8.90430392802342 1103 98-99 -8.068046177538161 1103 100-101 -9.58926161166131 1104 1 0.16281601402608104 1104 2 0.15184379494583666 1104 3 0.13998953928419766 1104 4 0.1682533032939375 1104 5 0.23313207254462753 1104 6 0.12377140671796383 1104 7 0.2085833137357298 1104 8 0.21883201138091835 1104 9 0.2640781433005017 1104 10-11 0.1789866698188476 1104 12-13 0.16953940658027022 1104 14-15 0.28662473723022686 1104 16-17 0.19970772580260387 1104 18-19 0.14430731888738535 1104 20-21 0.2239932841863208 1104 22-23 0.26603291762000225 1104 24-25 0.30231891133768585 1104 26-27 0.1826242460176033 1104 28-29 0.2371798320214964 1104 30-31 0.2687390408033181 1104 32-33 0.2654883906771701 1104 34-35 0.13661462321162787 1104 36-37 0.05456376396018925 1104 38-39 0.033870198370834714 1104 40-41 0.03317547036578361 1104 42-43 0.1386473205883192 1104 44-45 0.001035575020338797 1104 46-47 -0.08313467730031476 1104 48-49 -0.19135664960638366 1104 50-51 0.08668874316882835 1104 52-53 0.10884166537906026 1104 54-55 -0.05694909649607638 1104 56-57 0.45153443860251485 1104 58-59 -8.370166932214417 1104 60-61 -7.934846874336074 1104 62-63 -2.347795813079511 1104 64-65 -0.568212140657625 1104 66-67 -1.7823997880129774 1104 68-69 -3.5170282889328313 1104 70-71 -4.13170124853055 1104 72-73 -3.2340751068151654 1104 74-75 -3.9586403360677807 1104 76-77 -0.5319908937914626 1104 78-79 3.2569556898048795 1104 80-81 2.60654585244232 1104 82-83 1.1393796537849674 1104 84-85 0.7670644977894803 1104 86-87 0.7506160997516282 1104 88-89 0.39649998735680114 1104 90-91 1.8728906493128186 1104 92-93 1.5019776806593192 1104 94-95 -6.537355325684167 1104 96-97 -7.714310045614997 1104 98-99 -5.693974963527658 1104 100-101 -5.246418158426026 1105 1 0.22860548365509814 1105 2 0.19200401091214303 1105 3 0.23968842293161075 1105 4 0.17822740365092216 1105 5 0.28936504543758446 1105 6 0.2978744141728882 1105 7 0.255003885681127 1105 8 0.2674656256883807 1105 9 0.2875138735895817 1105 10-11 0.1973469494581508 1105 12-13 0.23322642432641771 1105 14-15 0.36304388839872814 1105 16-17 0.4058400577638892 1105 18-19 0.4011746383580572 1105 20-21 0.23360017073199657 1105 22-23 0.3557847192996846 1105 24-25 0.4437248907846012 1105 26-27 0.17311880684601277 1105 28-29 0.3694691597269255 1105 30-31 0.42785973503003305 1105 32-33 0.32837035288203964 1105 34-35 0.31639272518724937 1105 36-37 0.3260140792483597 1105 38-39 0.3576303753402499 1105 40-41 0.27828208564888257 1105 42-43 0.25256835559603275 1105 44-45 0.3149931105364274 1105 46-47 0.2706979942194607 1105 48-49 0.27939491671538974 1105 50-51 0.3777656067879107 1105 52-53 0.36773094658819616 1105 54-55 0.4046219190799789 1105 56-57 0.9400859354983311 1105 58-59 -8.247457164470863 1105 60-61 -7.373370638858585 1105 62-63 -1.856748402586657 1105 64-65 -0.12114368212042947 1105 66-67 -1.4224772948340991 1105 68-69 -3.03386372464956 1105 70-71 -3.6851295482489306 1105 72-73 -2.573315690778095 1105 74-75 -3.563662510152298 1105 76-77 -0.021995784839035082 1105 78-79 4.006931676312533 1105 80-81 3.405028168906558 1105 82-83 1.890169582110218 1105 84-85 1.495557628800622 1105 86-87 1.27417865209976 1105 88-89 1.322154163685795 1105 90-91 2.4440134257019572 1105 92-93 1.894225005556276 1105 94-95 -6.3031156503815104 1105 96-97 -7.568471355261044 1105 98-99 -5.4797601780172 1105 100-101 -4.961074946292941 1106 1 0.18847925202895865 1106 2 0.1278658156597352 1106 3 0.19285640088897793 1106 4 0.15798800809278646 1106 5 0.2138284687519061 1106 6 0.26973550775070265 1106 7 0.3073424311332147 1106 8 0.19250649626828675 1106 9 0.1837800003373431 1106 10-11 0.19567516600631762 1106 12-13 0.25585421882175297 1106 14-15 0.15818584855487217 1106 16-17 0.16444294800545123 1106 18-19 0.13289043022977154 1106 20-21 0.1200512570861818 1106 22-23 0.16350562086368825 1106 24-25 0.16532068233763653 1106 26-27 0.207295546652972 1106 28-29 0.31617116033059034 1106 30-31 0.3692691581505372 1106 32-33 0.3282302770434242 1106 34-35 0.2123995250674824 1106 36-37 0.18471895559184048 1106 38-39 0.2692003169006938 1106 40-41 0.31893917344093126 1106 42-43 0.3589934195100497 1106 44-45 0.28892211275979207 1106 46-47 0.1686326269066214 1106 48-49 0.3056320591602173 1106 50-51 0.28948449868213544 1106 52-53 0.2848551119696623 1106 54-55 0.4101499228016152 1106 56-57 1.031795624137672 1106 58-59 -8.219623066703978 1106 60-61 -7.7332692356496295 1106 62-63 -1.972587742759874 1106 64-65 -0.12705132262713548 1106 66-67 0.8131159455032666 1106 68-69 -2.312319616946265 1106 70-71 -2.310935904545591 1106 72-73 -0.9040275352138423 1106 74-75 -0.9611652675487079 1106 76-77 2.2204475573079456 1106 78-79 4.53728517266698 1106 80-81 3.572664760749465 1106 82-83 1.9622412230860604 1106 84-85 1.5240886265550735 1106 86-87 1.3407877891791422 1106 88-89 1.2360618792223228 1106 90-91 2.6941172219992104 1106 92-93 2.1867929083022943 1106 94-95 -6.553294325231807 1106 96-97 -7.968457408185106 1106 98-99 -5.868449502816361 1106 100-101 -5.220709093212427 1107 1 0.02185072438639324 1107 2 0.07228498159615526 1107 3 7.12128582048166E-4 1107 4 0.022619975032888817 1107 5 0.005590183865294307 1107 6 -0.012504907348727556 1107 7 0.033308871873288126 1107 8 0.0507566752201285 1107 9 0.05530708325662914 1107 10-11 0.029038476252019052 1107 12-13 0.13398951457587316 1107 14-15 0.038511442075609637 1107 16-17 0.008879883744157269 1107 18-19 0.13053285834197226 1107 20-21 0.20543923815046838 1107 22-23 0.07379919432175797 1107 24-25 0.04212329208927201 1107 26-27 0.15315473527810042 1107 28-29 0.05356927775427067 1107 30-31 -0.10488797351425916 1107 32-33 0.06539990450792743 1107 34-35 0.051578192414915236 1107 36-37 -0.021031704482062707 1107 38-39 0.03597726713650218 1107 40-41 -0.08824257033425198 1107 42-43 -0.15116221425973464 1107 44-45 -0.14958094808127953 1107 46-47 -0.07898455274548866 1107 48-49 -0.24983976645930994 1107 50-51 -0.10789036950713893 1107 52-53 -0.03515011571539617 1107 54-55 -0.06172973384293101 1107 56-57 0.5587206114790249 1107 58-59 -8.511436458801892 1107 60-61 -7.900196319571972 1107 62-63 -2.3330909103967663 1107 64-65 -0.4651225979236564 1107 66-67 0.2990129473236145 1107 68-69 -2.6497574927045697 1107 70-71 -2.5298336123394343 1107 72-73 -1.1506446103319234 1107 74-75 -1.098794844760171 1107 76-77 1.981178398692844 1107 78-79 4.044956131784929 1107 80-81 2.9794238448110555 1107 82-83 1.3651653930361753 1107 84-85 0.9789650310406159 1107 86-87 0.7665643969012699 1107 88-89 0.8123751654586364 1107 90-91 2.2784638454466766 1107 92-93 1.6978278866879393 1107 94-95 -6.766191799176232 1107 96-97 -8.330004785260998 1107 98-99 -6.248848309495958 1107 100-101 -5.338759681980948 1108 1 -0.0980202734376654 1108 2 -0.11092294565307625 1108 3 -0.11015806850207 1108 4 -0.19825396671042483 1108 5 -0.02677226181238268 1108 6 -0.014871699573859587 1108 7 -0.07951470020397977 1108 8 -0.12212686854552857 1108 9 -0.07363109443906524 1108 10-11 -0.05056757836285897 1108 12-13 -0.05304859333126899 1108 14-15 -0.2557220113306258 1108 16-17 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2312 98-99 -1.2222568067832817 2312 100-101 -0.7049207272543807 2313 1 -0.24917456917115288 2313 2 -0.2858736681980858 2313 3 -0.1646227276854404 2313 4 -0.14865706775212573 2313 5 -0.2537477649008153 2313 6 -0.24701793328651434 2313 7 -0.3640418658335278 2313 8 -0.3897834892008021 2313 9 -0.25682289833771677 2313 10-11 -0.41441255564365775 2313 12-13 -0.23311220995266524 2313 14-15 -0.387315735904032 2313 16-17 -0.34627292714669977 2313 18-19 -0.4147921310708327 2313 20-21 -0.3816712722571438 2313 22-23 -0.2936509858741516 2313 24-25 -0.327877672613333 2313 26-27 -0.3577986361576535 2313 28-29 -0.29604525906039925 2313 30-31 -0.4336962732994962 2313 32-33 -0.4839858742496119 2313 34-35 -0.4977195640429173 2313 36-37 -0.4517737333769958 2313 38-39 -0.5496097035283682 2313 40-41 -0.4572848277601409 2313 42-43 -0.3817129649745894 2313 44-45 -0.4473713794305212 2313 46-47 -0.513482290599633 2313 48-49 -0.6123393508741586 2313 50-51 -0.5371553224429135 2313 52-53 -0.3551174884196229 2313 54-55 -0.39111040567199495 2313 56-57 0.31714836061347285 2313 58-59 3.4025625750420936 2313 60-61 3.356803633373797 2313 62-63 0.6188698521870144 2313 64-65 0.014210828912553097 2313 66-67 -1.8682885124294835 2313 68-69 -4.687407419025636 2313 70-71 -3.442508996090318 2313 72-73 -2.66217503198326 2313 74-75 -3.480819528429464 2313 76-77 0.6299081978475733 2313 78-79 3.2320194607864856 2313 80-81 2.6980742803715536 2313 82-83 1.1386081204957001 2313 84-85 0.7960659561922263 2313 86-87 0.8397247807984023 2313 88-89 0.9419275943199921 2313 90-91 2.61220525464282 2313 92-93 3.847347388487176 2313 94-95 6.052034657765422 2313 96-97 1.3450911899307023 2313 98-99 3.0307979462316226 2313 100-101 4.099641149952417 2314 1 -0.31172033306795655 2314 2 -0.3227639432327898 2314 3 -0.3531213651803107 2314 4 -0.21995325465457682 2314 5 -0.340185898734056 2314 6 -0.37061243057108584 2314 7 -0.41448348199218543 2314 8 -0.49071167948242334 2314 9 -0.4680504006230777 2314 10-11 -0.42381730871117185 2314 12-13 -0.49457692648921636 2314 14-15 -0.6448535136632216 2314 16-17 -0.684943450439981 2314 18-19 -0.5646443448323524 2314 20-21 -0.5480275024377406 2314 22-23 -0.5866328908162828 2314 24-25 -0.5850435887607972 2314 26-27 -0.6436100943047549 2314 28-29 -0.5784670086779968 2314 30-31 -0.5962153063756617 2314 32-33 -0.6026458864405342 2314 34-35 -0.5837549058820173 2314 36-37 -0.5052949700904392 2314 38-39 -0.6952411834434855 2314 40-41 -0.6576873932218987 2314 42-43 -0.73371805533138 2314 44-45 -0.732929295569626 2314 46-47 -0.6939264822571758 2314 48-49 -0.8240633075792871 2314 50-51 -0.8180550358620309 2314 52-53 -0.6818379931198137 2314 54-55 -0.6463636915241437 2314 56-57 0.0630307861270154 2314 58-59 3.1684136554447058 2314 60-61 3.25174410240734 2314 62-63 0.5328238807517032 2314 64-65 -0.2096421069644947 2314 66-67 -1.9659313733312835 2314 68-69 -4.837962057415485 2314 70-71 -3.2914731506523154 2314 72-73 -2.9741056733452176 2314 74-75 -4.007086393329388 2314 76-77 0.35584732267284025 2314 78-79 3.2738963233618428 2314 80-81 2.5241165819823372 2314 82-83 0.8146640314747451 2314 84-85 0.5192043566715014 2314 86-87 0.48678141075636816 2314 88-89 0.4956052255022776 2314 90-91 2.2098644955781275 2314 92-93 3.8138129292530714 2314 94-95 6.445880452644882 2314 96-97 3.893405841313058 2314 98-99 6.255395737993997 2314 100-101 6.691110655034931 2315 1 -0.4294259673878429 2315 2 -0.536966138731021 2315 3 -0.5428029903498128 2315 4 -0.3701802105175531 2315 5 -0.4368633667597024 2315 6 -0.5071567808661612 2315 7 -0.5481927408152387 2315 8 -0.5828861055143335 2315 9 -0.6030079697443469 2315 10-11 -0.534332558999246 2315 12-13 -0.6041942740234703 2315 14-15 -0.7098075601408951 2315 16-17 -0.7903803112976746 2315 18-19 -0.6791238954618706 2315 20-21 -0.715584379338317 2315 22-23 -0.7196421303284666 2315 24-25 -0.5866773208319245 2315 26-27 -0.6005049264168321 2315 28-29 -0.6169585214412123 2315 30-31 -0.49800808792719664 2315 32-33 -0.6043729897876844 2315 34-35 -0.6390527178110474 2315 36-37 -0.5790306911819485 2315 38-39 -0.9110089393709728 2315 40-41 -0.8583886857774559 2315 42-43 -0.6345750020482157 2315 44-45 -0.6759364766695768 2315 46-47 -0.5839348538409084 2315 48-49 -0.5281516962244908 2315 50-51 -0.6721157314355892 2315 52-53 -0.6566187297767385 2315 54-55 -0.47909700830529545 2315 56-57 0.17433942700198202 2315 58-59 3.296713481222394 2315 60-61 3.469498650543418 2315 62-63 0.5234782648514305 2315 64-65 -0.032755264462739575 2315 66-67 -2.0392365785677136 2315 68-69 -5.019397566511756 2315 70-71 -3.7222534692605755 2315 72-73 -3.4539976915865687 2315 74-75 -1.0856445286201222 2315 76-77 1.5818174820618545 2315 78-79 3.5971185024352152 2315 80-81 2.5154513562042276 2315 82-83 0.8479819267164181 2315 84-85 0.6081617178294749 2315 86-87 0.5667300353338085 2315 88-89 0.6089725438580125 2315 90-91 2.311146599912039 2315 92-93 3.879368318579459 2315 94-95 6.519696282047072 2315 96-97 4.049631901660607 2315 98-99 6.255664710931182 2315 100-101 6.4961174775043045 2316 1 -0.48695858897740507 2316 2 -0.41092817906316625 2316 3 -0.45680061787057014 2316 4 -0.3040473516800688 2316 5 -0.5329487760123683 2316 6 -0.5747724392291502 2316 7 -0.5037087550501127 2316 8 -0.49540093350959324 2316 9 -0.6534231536114916 2316 10-11 -0.5733301865200033 2316 12-13 -0.5918870379617829 2316 14-15 -0.6589475364161004 2316 16-17 -0.8194431819975634 2316 18-19 -0.7292425194239129 2316 20-21 -0.6989770246526703 2316 22-23 -0.9204131860817242 2316 24-25 -0.7402953516741562 2316 26-27 -0.7849651873895453 2316 28-29 -0.7231269674673086 2316 30-31 -0.6448052409521097 2316 32-33 -0.6353634998707207 2316 34-35 -0.5164251970518521 2316 36-37 -0.4627792083824218 2316 38-39 -0.7123138029534175 2316 40-41 -0.7164847711867068 2316 42-43 -0.6052155714432317 2316 44-45 -0.6014999404892762 2316 46-47 -0.6181875228800493 2316 48-49 -0.6576000948010048 2316 50-51 -0.6034621134047526 2316 52-53 -0.44398527781945063 2316 54-55 -0.2624599976291435 2316 56-57 0.4264153463376914 2316 58-59 3.3555806223849096 2316 60-61 3.5107204773524323 2316 62-63 0.835014445159846 2316 64-65 0.36834200718613985 2316 66-67 -1.924912735151345 2316 68-69 -3.011687008979134 2316 70-71 -3.139216695832342 2316 72-73 -0.6532859478143287 2316 74-75 5.545583229386995 2316 76-77 5.680127090602777 2316 78-79 4.662065121652297 2316 80-81 3.1199887227522716 2316 82-83 1.3986936704886617 2316 84-85 0.9827168779718249 2316 86-87 0.9211191219293049 2316 88-89 0.8958942520430639 2316 90-91 2.4767753069108522 2316 92-93 4.136337476349329 2316 94-95 6.6690141942653405 2316 96-97 3.6866425778171923 2316 98-99 6.108719277953721 2316 100-101 6.359551641205369 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 8.0 3 14.0 4 37.0 5 137.0 6 303.0 7 774.0 8 1699.0 9 2881.0 10 4658.0 11 7345.0 12 10669.0 13 14502.0 14 17778.0 15 23810.0 16 32000.0 17 41837.0 18 45091.0 19 44443.0 20 46801.0 21 48528.0 22 48248.0 23 48766.0 24 51799.0 25 54995.0 26 68054.0 27 95348.0 28 136146.0 29 181999.0 30 217447.0 31 239840.0 32 246318.0 33 243763.0 34 248282.0 35 263306.0 36 263435.0 37 198327.0 38 76505.0 39 4772.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.57089654207322 19.693799283674622 14.143102694867302 23.59220147938485 2 15.426535787887937 21.46062169069906 39.479949905701936 23.63289261571106 3 18.848831607636622 25.90749209743092 30.53259026086727 24.71108603406519 4 12.597862185361958 17.416738570577746 37.78873613546862 32.19666310859168 5 13.019035697670155 38.4977677605922 34.61234053133274 13.8708560104049 6 30.9847508715084 37.34972357551891 17.83473264118601 13.830792911786688 7 27.443679852441626 33.356276592761 21.56209940722581 17.63794414757157 8 25.827409438987626 35.47173160733094 20.264536088461142 18.436322865220294 9 26.599079739925063 16.17278716057367 20.306533384587212 36.92159971491405 10-11 24.480567796176746 26.798012977349856 28.216711513809678 20.504707712663723 12-13 25.7638340100275 24.18899812417407 28.391607122529216 21.655560743269216 14-15 22.5217543947639 25.327256313162977 26.807168886431036 25.343820405642088 16-17 21.91364664641148 28.64586770423907 27.30522090208614 22.135264747263307 18-19 21.747536583748232 27.4595804397012 29.445260734581936 21.347622241968633 20-21 22.67045351432771 26.588141546492274 29.179553002393867 21.56185193678615 22-23 22.252228471309103 26.725372154296167 28.948052655110345 22.07434671928438 24-25 22.17127809039741 26.75186147448973 29.147938635189274 21.928921799923582 26-27 22.154437240846594 26.86406911494405 29.1248791724183 21.856614471791058 28-29 21.944134140886998 27.03669809077934 29.200815868756198 21.818351899577465 30-31 22.071953755516414 26.8592794197133 29.16906175940488 21.899705065365406 32-33 21.908662580306775 26.903302883347024 29.018687911206392 22.16934662513981 34-35 22.387322939819338 26.827570573297503 28.880244492194308 21.904861994688854 36-37 22.405334126842718 26.660197935895823 28.946915179978728 21.98755275728273 38-39 22.263698514779225 26.85479220977365 29.082738956613746 21.798770318833384 40-41 22.311871103766144 26.716621685352738 29.095627895548237 21.875879315332885 42-43 22.101963229613943 26.928361258292693 28.794144428147405 22.17553108394596 44-45 22.292380080430796 27.072665155286398 28.459676929255778 22.17527783502703 46-47 22.28281870177703 27.123098740959794 28.100042208000232 22.494040349262946 48-49 22.47729773115184 27.10176273332394 28.101261935233744 22.319677600290472 50-51 22.363536939459053 27.304103525784857 28.029779942655185 22.302579592100905 52-53 22.56564938853364 27.286382983078894 27.91432504276583 22.233642585621634 54-55 22.111908485781377 27.1192581801975 28.1413980513612 22.62743528265992 56-57 22.23431267665161 27.550915286472776 27.91724449903585 22.297527537839766 58-59 21.48387250048969 26.863936626046726 29.417412386410057 22.234778487053525 60-61 21.710181315450747 27.062675131232556 28.98458315456043 22.242560398756268 62-63 22.495311527875188 27.106982956844938 28.076767038824197 22.32093847645568 64-65 22.29406718141622 27.119530785982978 28.0434402428562 22.542961789744606 66-67 22.04471713862346 27.020438999455482 28.651239506782282 22.28360435513877 68-69 21.503270799793814 26.490624022526397 30.089128703252694 21.916976474427095 70-71 22.245508451178708 26.522981008805367 29.066988857564773 22.16452168245115 72-73 21.56987684144974 26.64906698984058 29.310431696481654 22.470624472228025 74-75 21.757108533581953 26.827901474671933 28.853077496299118 22.561912495446997 76-77 20.66042690101241 27.20139530555131 29.140614093982038 22.997563699454243 78-79 21.80242362888015 27.093442086889695 28.277550516600957 22.826583767629195 80-81 21.650452860413825 27.459480888625404 27.930933691108216 22.959132559852556 82-83 22.02199650703493 27.414199467245286 27.579724304549863 22.984079721169923 84-85 21.984774460372368 27.151255439666617 27.509068654503192 23.35490144545782 86-87 22.053540450161343 27.06655014502786 27.72453334288032 23.15537606193047 88-89 22.033607492220764 27.049175865284948 27.603179963727413 23.31403667876688 90-91 21.686097063318435 27.11205319094877 27.952542746569176 23.249306999163615 92-93 21.452756517699946 27.183073342839155 28.001539111151757 23.362631028309146 94-95 20.49291982080487 27.12940756433643 28.583744346864005 23.793928267994698 96-97 20.26309597698711 27.0893332611092 28.635287516063862 24.01228324583983 98-99 20.870057382658754 26.825986199073764 28.17529719873938 24.1286592195281 100-101 20.131771741640588 26.962275469764897 28.72454085275701 24.181411935837506 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 662.0 1 512.5 2 398.0 3 459.0 4 490.0 5 570.5 6 774.5 7 1144.0 8 1643.5 9 2051.5 10 2238.5 11 2363.0 12 2604.5 13 2830.5 14 2992.0 15 3304.5 16 3682.5 17 3923.5 18 4253.0 19 4777.0 20 5437.0 21 6289.0 22 7423.5 23 8915.0 24 10924.0 25 13691.5 26 17422.0 27 21774.0 28 26633.0 29 31952.5 30 37273.5 31 43143.0 32 49459.5 33 55571.0 34 61539.0 35 67532.0 36 74157.0 37 80524.5 38 85395.0 39 89257.5 40 93267.5 41 98083.0 42 102759.5 43 106736.5 44 110737.5 45 114869.0 46 118381.0 47 123176.0 48 129127.0 49 128092.5 50 121666.5 51 116103.5 52 110289.0 53 104658.5 54 97879.5 55 89169.0 56 79748.0 57 70011.5 58 60803.0 59 51686.0 60 42423.5 61 33688.5 62 25892.0 63 19544.0 64 14423.5 65 10242.5 66 7090.0 67 4940.0 68 3305.5 69 2034.0 70 1318.0 71 941.5 72 659.5 73 464.0 74 316.5 75 219.0 76 129.0 77 54.5 78 23.0 79 18.5 80 13.5 81 7.0 82 2.5 83 5.0 84 4.5 85 1.5 86 2.0 87 1.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02497801637594389 2 0.012538502275903144 3 1.6498029310398873E-4 4 0.0 5 0.0690277546347089 6 0.0 7 0.0 8 3.2996058620797744E-5 9 0.0 10-11 0.0 12-13 0.0 14-15 4.289487620703707E-4 16-17 3.2996058620797744E-5 18-19 5.279369379327639E-4 20-21 0.0 22-23 0.0 24-25 9.898817586239325E-5 26-27 1.1548620517279211E-4 28-29 0.0010063797879343312 30-31 0.005378357555190032 32-33 0.005823804346570803 34-35 0.0018807753413854717 36-37 0.09125060011581616 38-39 0.08456889824510462 40-41 0.09044219667960662 42-43 0.09397277495203199 44-45 0.09796529804514852 46-47 0.09248795231409608 48-49 0.08244065246406317 50-51 0.07648486388300918 52-53 0.06473826701400517 54-55 0.05370108540534833 56-57 0.06945670339677926 58-59 0.6962333349281429 60-61 0.3323692984872957 62-63 0.003085131481044589 64-65 0.0021447438103518535 66-67 1.0755890208914547 68-69 2.3172142087627634 70-71 2.299412835136843 72-73 1.7661800297954409 74-75 1.258304695504122 76-77 0.7260782699506544 78-79 0.5569239754311348 80-81 0.039578772315646896 82-83 6.599211724159549E-5 84-85 0.0011053679637967244 86-87 0.0013363403741423086 88-89 4.949408793119662E-5 90-91 0.327502379840728 92-93 0.05424552037259149 94-95 0.9281131368857989 96-97 1.6235875624656635 98-99 0.6311486092986194 100-101 0.5548947178259557 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3030665.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.02227724134993 #Duplication Level Percentage of deduplicated Percentage of total 1 71.03688248612434 35.53426630082107 2 14.984612326281624 14.99128864278818 3 5.651372829200509 8.480836153695002 4 2.7977435689698944 5.597980178288635 5 1.541151488038897 3.854595350280035 6 0.9568488507359644 2.8718255093748883 7 0.6326681805477842 2.215325219039919 8 0.44609468173441535 1.7851737476488554 9 0.32760943688789884 1.474899307100011 >10 1.487698715094292 12.724677678589883 >50 0.08438541552884181 2.9219641294424723 >100 0.047779884185434184 4.429364098985806 >500 0.003831075985648212 1.3295130895850449 >1k 0.0011229015817575928 0.9307384615141933 >5k 1.3210606844206975E-4 0.4871929644299329 >10k+ 6.605303422103487E-5 0.37035916841608124 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11209 0.3698528210805219 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8336 0.27505514466297004 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6409 0.21147173970069277 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 3417 0.11274753230726589 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 3.2996058620797744E-5 0.0 0.0 0.0 0.0 7 1.3198423448319098E-4 0.0 0.0 0.0 0.0 8 1.3198423448319098E-4 0.0 0.0 3.2996058620797744E-5 0.0 9 1.3198423448319098E-4 0.0 0.0 3.2996058620797744E-5 0.0 10-11 1.6498029310398873E-4 0.0 0.0 1.3198423448319098E-4 0.0 12-13 2.1447438103518534E-4 0.0 0.0 1.4848226379358986E-4 0.0 14-15 2.6396846896638195E-4 0.0 0.0 1.6498029310398873E-4 0.0 16-17 2.6396846896638195E-4 0.0 0.0 2.6396846896638195E-4 0.0 18-19 3.134625568975786E-4 0.0 0.0 3.4645861551837635E-4 0.0 20-21 3.6295664482877523E-4 0.0 0.0 5.279369379327639E-4 0.0 22-23 3.9595270344957293E-4 0.0 0.0 7.75407377588747E-4 0.0 24-25 5.609329965535617E-4 1.6498029310398872E-5 0.0 0.0010558738758655278 0.0 26-27 5.609329965535617E-4 6.599211724159549E-5 0.0 0.001583810813798292 0.0 28-29 5.939290551743594E-4 6.599211724159549E-5 0.0 0.0029201511879406008 0.0 30-31 5.939290551743594E-4 8.249014655199437E-5 0.0 0.008166524508647442 0.0 32-33 5.939290551743594E-4 9.898817586239323E-5 0.0 0.018378804651784344 0.0 34-35 6.104270844847584E-4 9.898817586239323E-5 0.0 0.03479434381563122 0.0 36-37 6.929172310367527E-4 1.154862051727921E-4 0.0 0.061686131591581386 0.0 38-39 7.589093482783481E-4 1.6498029310398873E-4 0.0 0.10268373442792258 0.0 40-41 7.589093482783481E-4 1.6498029310398873E-4 0.0 0.14851525985221065 0.0 42-43 8.084034362095447E-4 1.979763517247865E-4 0.0 0.19660701529202337 0.0 44-45 8.578975241407414E-4 2.3097241034558422E-4 0.0 0.2547625686111794 0.0 46-47 9.568857000031347E-4 2.3097241034558422E-4 0.0 0.3227839434579539 0.0 48-49 0.0010228778172447302 2.3097241034558422E-4 0.0 0.3925706074409412 0.0 50-51 0.0010228778172447302 2.474704396559831E-4 0.0 0.46430403888255545 0.0 52-53 0.0010228778172447302 2.6396846896638195E-4 0.0 0.535592023532789 0.0 54-55 0.0010228778172447302 2.6396846896638195E-4 0.0 0.6145680898416683 0.0 56-57 0.0011548620517279212 2.6396846896638195E-4 0.0 0.6913664162815751 0.0 58-59 0.0011548620517279212 2.8046649827678083E-4 0.0 0.7491920090145232 0.0 60-61 0.0011548620517279212 2.969645275871797E-4 1.6498029310398872E-5 0.802744612156078 0.0 62-63 0.0011548620517279212 3.2996058620797747E-4 3.2996058620797744E-5 0.8510178459183051 0.0 64-65 0.00117136008103832 3.9595270344957293E-4 6.599211724159549E-5 0.904207492415031 0.0 66-67 0.0011878581103487188 3.9595270344957293E-4 6.599211724159549E-5 0.9485706932306937 0.0 68-69 0.0012208541689695165 3.9595270344957293E-4 6.599211724159549E-5 1.0020573042550067 0.0 70-71 0.0012208541689695165 3.9595270344957293E-4 6.599211724159549E-5 1.0881275231673575 0.0 72-73 0.0012208541689695165 3.9595270344957293E-4 6.599211724159549E-5 1.202953807167734 0.0 74-75 0.0012208541689695165 3.9595270344957293E-4 6.599211724159549E-5 1.3563524836958227 0.0 76-77 0.0012208541689695165 3.9595270344957293E-4 6.599211724159549E-5 1.5455683818567874 0.0 78-79 0.0012208541689695165 3.9595270344957293E-4 6.599211724159549E-5 1.786703578257577 0.0 80-81 0.001435328550004702 3.9595270344957293E-4 6.599211724159549E-5 2.0880400836120128 0.0 82-83 0.0014848226379358986 3.9595270344957293E-4 6.599211724159549E-5 2.3885681855302385 0.0 84-85 0.0015178186965566962 4.124507327599718E-4 6.599211724159549E-5 2.655506299772492 0.0 86-87 0.001550814755177494 4.289487620703707E-4 6.599211724159549E-5 2.902877751252613 0.0 88-89 0.001550814755177494 4.289487620703707E-4 6.599211724159549E-5 3.14307255998271 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 17155 0.0 26.819593 1 GTACATG 12835 0.0 25.000652 1 GGTATCA 12210 0.0 24.88906 1 TACATGG 12785 0.0 24.611694 2 ACATGGG 12365 0.0 24.186647 3 TCAACGC 20225 0.0 22.460442 4 ATCAACG 20315 0.0 22.407377 3 CAACGCA 20455 0.0 22.207891 5 AACGCAG 20535 0.0 22.174911 6 TATCAAC 20735 0.0 22.091824 2 CATGGGA 8740 0.0 21.91266 4 AGTACTT 10105 0.0 20.526955 12-13 GAGTACT 9850 0.0 20.292604 12-13 ACGCAGA 22720 0.0 19.855604 7 CGCAGAG 22595 0.0 19.735962 8 CATGGGG 4410 0.0 18.61192 4 GCAGAGT 23795 0.0 18.18627 9 GTACTTT 10960 0.0 18.172903 14-15 ATGGGAG 4040 0.0 18.098011 5 AGAGTAC 19725 0.0 17.445866 10-11 >>END_MODULE