##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939641_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 881419 Sequences flagged as poor quality 0 Sequence length 101 %GC 39 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.325757670302092 31.0 30.0 33.0 26.0 34.0 2 30.340101586192265 31.0 30.0 33.0 26.0 34.0 3 30.33094362613014 31.0 30.0 34.0 26.0 34.0 4 33.90689558541398 35.0 35.0 37.0 28.0 37.0 5 33.894177457032356 35.0 35.0 37.0 28.0 37.0 6 34.13014922528332 36.0 35.0 37.0 30.0 37.0 7 33.981639833041946 36.0 35.0 37.0 29.0 37.0 8 34.02882284135014 36.0 35.0 37.0 29.0 37.0 9 35.15866233879687 38.0 35.0 39.0 28.0 39.0 10-11 35.082902115792834 37.0 34.5 39.0 28.0 39.0 12-13 34.91662421617869 37.0 34.0 39.0 27.0 39.0 14-15 35.2852343777477 38.0 33.5 40.0 27.0 41.0 16-17 35.156031921254254 38.0 33.5 40.0 27.0 41.0 18-19 35.407190564305964 38.0 34.0 40.0 27.0 41.0 20-21 35.61330819961903 38.0 34.0 40.0 27.0 41.0 22-23 35.516748561127 38.0 34.0 40.0 27.0 41.0 24-25 35.33659474098017 38.0 34.0 40.0 27.0 41.0 26-27 35.18220222164487 38.0 34.0 40.0 26.0 41.0 28-29 34.980316398897685 38.0 34.0 40.0 25.0 41.0 30-31 34.66017240381703 38.0 33.5 40.0 25.0 41.0 32-33 34.30692950798655 37.0 33.0 40.0 24.0 41.0 34-35 34.007938903064264 37.0 33.0 40.0 23.5 41.0 36-37 33.512960918700415 37.0 33.0 40.0 21.0 41.0 38-39 33.155612143600266 36.5 32.5 40.0 19.5 41.0 40-41 32.73963064104586 36.0 31.0 39.5 17.0 41.0 42-43 32.3961021943026 36.0 31.0 39.0 15.0 41.0 44-45 32.02205818118284 36.0 30.5 39.0 11.5 41.0 46-47 31.291856653872905 35.0 30.0 39.0 6.5 41.0 48-49 30.74725697993803 35.0 28.5 39.0 2.0 40.0 50-51 30.041656692220158 35.0 27.5 38.5 2.0 40.0 52-53 29.900148510526776 35.0 27.5 38.5 2.0 40.0 54-55 29.985997578904016 35.0 27.5 39.0 2.0 40.0 56-57 29.997314557548677 35.0 28.0 39.0 2.0 40.0 58-59 29.678513283693682 35.0 27.0 39.0 2.0 40.0 60-61 29.209530881453656 35.0 26.0 38.0 2.0 40.0 62-63 28.66981991538644 34.0 26.0 38.0 2.0 40.0 64-65 27.87201830230571 33.5 24.0 37.5 2.0 39.5 66-67 27.376148006793592 33.0 22.5 36.5 2.0 39.0 68-69 26.979995325719095 33.0 22.0 36.0 2.0 39.0 70-71 26.345672149114097 32.5 21.0 35.5 2.0 38.5 72-73 25.573089529497324 31.5 19.0 35.0 2.0 37.5 74-75 25.096902835087512 31.0 18.0 35.0 2.0 37.0 76-77 24.66041122326612 31.0 14.0 35.0 2.0 36.5 78-79 24.084731552190277 31.0 7.5 34.0 2.0 36.0 80-81 23.50260318872182 30.5 4.5 34.0 2.0 35.5 82-83 22.883282525110076 30.0 2.0 34.0 2.0 35.0 84-85 22.277997184086118 29.0 2.0 34.0 2.0 35.0 86-87 21.485993608034317 29.0 2.0 34.0 2.0 35.0 88-89 20.52774049572337 27.5 2.0 33.5 2.0 35.0 90-91 19.30431100305303 25.5 2.0 33.0 2.0 34.5 92-93 17.956408926968898 23.0 2.0 32.0 2.0 34.0 94-95 17.72981692021615 23.0 2.0 32.0 2.0 34.0 96-97 17.018918357784436 19.5 2.0 32.0 2.0 34.0 98-99 16.059221550704034 8.5 2.0 31.5 2.0 34.0 100-101 14.507549190566575 2.0 2.0 30.0 2.0 33.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.0079171028231713 1101 2 0.8806328545257891 1101 3 1.0642409599531781 1101 4 1.2143003771107317 1101 5 1.060745493492746 1101 6 0.9993539021962476 1101 7 1.1021378372356807 1101 8 1.0641054591397392 1101 9 1.3875409714122284 1101 10-11 1.0984289652723618 1101 12-13 1.0483795720423714 1101 14-15 1.1436003917648634 1101 16-17 1.2116442906073246 1101 18-19 1.335514947088889 1101 20-21 1.6017893633036877 1101 22-23 1.4801273677120221 1101 24-25 1.3618350318268213 1101 26-27 1.383230217678559 1101 28-29 1.4346335439006879 1101 30-31 1.5526320959675317 1101 32-33 1.2398432616085273 1101 34-35 1.4676398056745157 1101 36-37 1.3444157953061122 1101 38-39 1.3885359756903526 1101 40-41 1.4896895752972625 1101 42-43 1.3343139170910874 1101 44-45 1.2620991446822316 1101 46-47 0.9158047856168565 1101 48-49 1.0012465294003938 1101 50-51 0.7232264885695656 1101 52-53 0.5464820149506195 1101 54-55 0.31471784305661643 1101 56-57 0.5786666049498166 1101 58-59 0.39797215975215394 1101 60-61 0.4039270559289747 1101 62-63 0.565759710399707 1101 64-65 0.52227682109773 1101 66-67 0.5387680544993607 1101 68-69 0.6087001301448609 1101 70-71 0.3917051592199776 1101 72-73 0.6035685214546973 1101 74-75 -0.12165559558888361 1101 76-77 -0.10387581279196922 1101 78-79 0.3119851832387326 1101 80-81 -0.03021792900970155 1101 82-83 -0.14998069572387607 1101 84-85 0.03626662066043451 1101 86-87 -0.6918153910171831 1101 88-89 -0.41382557429895783 1101 90-91 -3.93790408084282 1101 92-93 -8.389658395324513 1101 94-95 -6.492190915251852 1101 96-97 -4.1035321732392696 1101 98-99 -3.2347141119706553 1101 100-101 -2.983491759836257 1102 1 1.0129487435084634 1102 2 1.04245052186128 1102 3 1.0674153181360246 1102 4 1.2243044929722302 1102 5 0.9327807870051146 1102 6 0.9914668825944588 1102 7 0.938442308090238 1102 8 0.7590583839738159 1102 9 1.0456980944250418 1102 10-11 0.7572111847506875 1102 12-13 0.8274825714764376 1102 14-15 1.1602194687829197 1102 16-17 1.157134849850003 1102 18-19 0.9408076877381646 1102 20-21 1.0413607992248686 1102 22-23 1.329830253959905 1102 24-25 1.1780605295888265 1102 26-27 1.2192735885691306 1102 28-29 1.5835312647423834 1102 30-31 1.3950717728724058 1102 32-33 1.2285002045523967 1102 34-35 1.2900339508615346 1102 36-37 1.392617678312753 1102 38-39 1.5949657230793477 1102 40-41 1.4523252701061296 1102 42-43 1.4928671917733922 1102 44-45 1.543922471327292 1102 46-47 1.3586714831524844 1102 48-49 1.0818260941480382 1102 50-51 1.0440205925979669 1102 52-53 1.1870193921178789 1102 54-55 1.0828302060755988 1102 56-57 1.1743938262288225 1102 58-59 1.2166774640686597 1102 60-61 1.1210979156295444 1102 62-63 1.0744351747203318 1102 64-65 0.8146444704263267 1102 66-67 0.6531928628545565 1102 68-69 0.7778839033608911 1102 70-71 0.8109030807099167 1102 72-73 0.798763253151975 1102 74-75 0.5011488495415399 1102 76-77 0.4533951137913519 1102 78-79 0.518457375449465 1102 80-81 0.30373021113538456 1102 82-83 -0.06071666320857361 1102 84-85 -0.6553717494584106 1102 86-87 -1.3302369581314508 1102 88-89 -2.072934490283938 1102 90-91 -5.087002449932701 1102 92-93 -9.342768443171405 1102 94-95 -8.30923315428051 1102 96-97 -5.617245709278784 1102 98-99 -4.517777341892968 1102 100-101 -3.5842570528341486 1103 1 0.6080516589649498 1103 2 0.6570453989352174 1103 3 0.6060867397296263 1103 4 0.37248410526011355 1103 5 0.696759326808376 1103 6 0.6315411476903918 1103 7 0.5717977878984328 1103 8 0.5431748971908021 1103 9 0.5201070986740106 1103 10-11 0.5529808579107325 1103 12-13 0.5243093667852392 1103 14-15 0.624256175908485 1103 16-17 0.5740724388079315 1103 18-19 0.5645708114940646 1103 20-21 0.7042621274881427 1103 22-23 0.7298316982952215 1103 24-25 0.5199314165157531 1103 26-27 0.5747921312085325 1103 28-29 0.969311258334784 1103 30-31 1.1589019400476488 1103 32-33 0.9217608046605861 1103 34-35 0.7902617619319727 1103 36-37 0.952581228541483 1103 38-39 1.0179872463016366 1103 40-41 0.9309688028977945 1103 42-43 0.8746654155035642 1103 44-45 0.9490165106867394 1103 46-47 1.2840755031315823 1103 48-49 1.0737227165042498 1103 50-51 0.6193774747244483 1103 52-53 0.9057528150875349 1103 54-55 1.042485981578622 1103 56-57 0.9128391436937235 1103 58-59 0.7427746336966514 1103 60-61 0.5328424626072064 1103 62-63 0.652259244174477 1103 64-65 0.8581360328820118 1103 66-67 0.8611869962271079 1103 68-69 0.6269383889411735 1103 70-71 0.7849431229370332 1103 72-73 1.0552404405321631 1103 74-75 0.6838612062240834 1103 76-77 0.6140071705773664 1103 78-79 0.5757659747593351 1103 80-81 0.05444975272961372 1103 82-83 0.3146404815243322 1103 84-85 -0.10861139357418637 1103 86-87 -0.5273677203728475 1103 88-89 -0.5860385507044192 1103 90-91 -1.9556631309236714 1103 92-93 -8.235761578639554 1103 94-95 -4.928523434166433 1103 96-97 -2.5279293263145863 1103 98-99 -1.5613621296281952 1103 100-101 -1.2252655402736838 1104 1 0.7664928123243158 1104 2 0.6666367325444718 1104 3 0.6727726010601778 1104 4 0.8945753638902119 1104 5 0.940879461410951 1104 6 0.6770552947575084 1104 7 0.8466821700401965 1104 8 0.751219860690945 1104 9 0.7200987010085669 1104 10-11 0.6089524977948457 1104 12-13 0.6215507336852397 1104 14-15 0.8424017314440491 1104 16-17 0.7678755615398174 1104 18-19 0.7107837642931898 1104 20-21 0.8539487146169265 1104 22-23 0.5317931530108098 1104 24-25 0.6519637115377321 1104 26-27 0.7635404591733632 1104 28-29 1.0091443847256514 1104 30-31 1.1085813291774755 1104 32-33 1.2723657364895402 1104 34-35 1.1996863418987473 1104 36-37 1.1268765744353288 1104 38-39 1.0487862841691111 1104 40-41 0.9410028134428643 1104 42-43 0.9007161733928726 1104 44-45 0.5933171950964748 1104 46-47 0.659573514684368 1104 48-49 0.4809926954501016 1104 50-51 0.8016506027948438 1104 52-53 0.7151582003904231 1104 54-55 0.7443670586391988 1104 56-57 0.5425128943910309 1104 58-59 0.1991355653576541 1104 60-61 0.581875231497456 1104 62-63 0.33656880611625795 1104 64-65 0.4674028567049895 1104 66-67 0.44356269578262797 1104 68-69 0.5191531532367009 1104 70-71 0.27175099034989003 1104 72-73 0.040444953677504 1104 74-75 0.14661516062475854 1104 76-77 0.1886678149182366 1104 78-79 -0.06270193116281675 1104 80-81 0.06454674576555064 1104 82-83 0.07556134552413951 1104 84-85 -0.1365696211869114 1104 86-87 -0.17903555671434646 1104 88-89 -0.7107936687815943 1104 90-91 -0.8047822758013972 1104 92-93 -3.7060518379474665 1104 94-95 -1.63121788510907 1104 96-97 -1.0854641316387053 1104 98-99 -0.8463129133103333 1104 100-101 -0.7387887808128131 1105 1 0.916197632777088 1105 2 0.8041879620526693 1105 3 0.840388233378679 1105 4 0.7551005883242468 1105 5 0.7379008314343736 1105 6 0.8107594321501708 1105 7 0.8187877028270805 1105 8 0.8455138692779869 1105 9 1.0405075612739836 1105 10-11 0.9385247314014009 1105 12-13 0.8117988308749418 1105 14-15 0.7789582546680904 1105 16-17 1.0159117637256045 1105 18-19 0.8668105500074716 1105 20-21 0.637033218910446 1105 22-23 0.6963716073433588 1105 24-25 0.7373858670802704 1105 26-27 0.7336730460195753 1105 28-29 0.7803576702447756 1105 30-31 0.9494014892086966 1105 32-33 1.127661281961828 1105 34-35 0.9249290721875738 1105 36-37 0.9085791017577307 1105 38-39 1.0621510870574795 1105 40-41 1.052141572225068 1105 42-43 1.4487316398253398 1105 44-45 1.430454319413414 1105 46-47 1.1792456458319123 1105 48-49 0.9761277217967042 1105 50-51 0.6508510565419172 1105 52-53 0.6526588102655175 1105 54-55 0.6435412877570776 1105 56-57 0.7766024728453651 1105 58-59 0.946163644007143 1105 60-61 0.5141553105372694 1105 62-63 0.5000135026424104 1105 64-65 0.677698768102335 1105 66-67 0.6214809481245531 1105 68-69 0.3187219715427112 1105 70-71 0.5317517222000063 1105 72-73 0.5100585368156807 1105 74-75 0.20958720395028507 1105 76-77 -0.28560704359073696 1105 78-79 -0.4893925049333099 1105 80-81 -0.3445684474428816 1105 82-83 -0.10350652137671901 1105 84-85 -0.15249345998472563 1105 86-87 -0.26937635491887235 1105 88-89 -0.3776780608580985 1105 90-91 -1.3451273431316046 1105 92-93 -6.597024466753085 1105 94-95 -3.021441587294863 1105 96-97 -1.783882115635583 1105 98-99 -1.3080280040597483 1105 100-101 -1.1033050278854137 1106 1 0.7712547170862223 1106 2 0.9207350932002107 1106 3 0.824763233378679 1106 4 1.1152623272001279 1106 5 0.7582096721837885 1106 6 0.8385697365998155 1106 7 0.7349725681666612 1106 8 0.7932963634231882 1106 9 0.9414882404308926 1106 10-11 0.8851820059915667 1106 12-13 0.9117146681114647 1106 14-15 1.0440801077125812 1106 16-17 1.225330674732632 1106 18-19 0.8099640921620406 1106 20-21 0.7129260760533 1106 22-23 0.8904582583972243 1106 24-25 0.9597310807805073 1106 26-27 0.7743913569094971 1106 28-29 0.9022747516265142 1106 30-31 0.9364111496302385 1106 32-33 0.9622174148658118 1106 34-35 0.7798580827262143 1106 36-37 0.913098276230798 1106 38-39 0.8372718423267997 1106 40-41 0.7666468415459065 1106 42-43 0.8390456035255767 1106 44-45 0.6609596619192715 1106 46-47 0.6728053648014694 1106 48-49 0.6764259507194232 1106 50-51 0.63314943183466 1106 52-53 0.5730426656519363 1106 54-55 0.8767636238226508 1106 56-57 0.9114824494261597 1106 58-59 0.9289560181289254 1106 60-61 0.5201656296239179 1106 62-63 0.6608467140007228 1106 64-65 0.46182127981193943 1106 66-67 0.5813847098341505 1106 68-69 0.615679304680885 1106 70-71 0.4250296788744805 1106 72-73 0.599344251101396 1106 74-75 0.5291678538331901 1106 76-77 0.28777007877459937 1106 78-79 0.24256738968027491 1106 80-81 0.6390198136812408 1106 82-83 0.7037424540331187 1106 84-85 0.41983162783729 1106 86-87 0.2955936392263254 1106 88-89 0.3261696411325339 1106 90-91 -0.07105862240561223 1106 92-93 -2.6738504327952395 1106 94-95 -1.2468697196133611 1106 96-97 -0.5602104235981109 1106 98-99 -0.31879534890752304 1106 100-101 -0.29089650915005016 1107 1 0.08465876723087007 1107 2 0.20505455869132305 1107 3 0.06635987203414118 1107 4 -0.03947661755810117 1107 5 -0.06054979881772482 1107 6 0.06706195315857144 1107 7 -0.11982836860148893 1107 8 -0.07347247525982681 1107 9 -0.2559735311629936 1107 10-11 -0.08573340935490137 1107 12-13 -0.06891282878893179 1107 14-15 0.021375534079858483 1107 16-17 0.12554936876762213 1107 18-19 0.13368292395704628 1107 20-21 0.21345128613733522 1107 22-23 0.14017412835176657 1107 24-25 0.1824029364080033 1107 26-27 0.03353517837251019 1107 28-29 -0.14365099572160744 1107 30-31 -0.08145460322827347 1107 32-33 -0.22347448484489263 1107 34-35 -0.13684876394687961 1107 36-37 0.04690630764007864 1107 38-39 -0.04124964823663646 1107 40-41 0.08076552180489216 1107 42-43 -0.2402783391662595 1107 44-45 -0.44976495579666675 1107 46-47 -0.6364056146722916 1107 48-49 -0.38826959963186525 1107 50-51 -0.487115067407661 1107 52-53 -0.5005384061210343 1107 54-55 -0.5503268845118328 1107 56-57 -0.6543324011042131 1107 58-59 -0.4166580471693244 1107 60-61 -0.23568056131146875 1107 62-63 -0.5913402263491889 1107 64-65 -0.4049561688724559 1107 66-67 -0.5390692094384768 1107 68-69 -0.7264132700539285 1107 70-71 -0.7127992231781448 1107 72-73 -0.5729246564616304 1107 74-75 -0.6649532372261824 1107 76-77 -0.6041141339268741 1107 78-79 -0.7374229671181816 1107 80-81 -1.0801908213924598 1107 82-83 -1.416203475158234 1107 84-85 -1.3055265482200191 1107 86-87 -1.4556289809692942 1107 88-89 -1.5744020629589386 1107 90-91 -1.5524868494341284 1107 92-93 -4.94648612641695 1107 94-95 -2.834269843597383 1107 96-97 -1.734184111433903 1107 98-99 -1.647215241454706 1107 100-101 -1.4746942085576809 1108 1 0.2273875131022436 1108 2 0.18588909482400595 1108 3 0.04988742425629411 1108 4 -0.16410360217702902 1108 5 -1.0334679966553466 1108 6 -0.18180793199253742 1108 7 0.09596268965532317 1108 8 0.06786493430885088 1108 9 0.23499329813985526 1108 10-11 0.27851666369855366 1108 12-13 0.19898858889089155 1108 14-15 0.11527855893573502 1108 16-17 0.03864951398903571 1108 18-19 0.22838123531531807 1108 20-21 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-0.38408056472989216 2312 74-75 -0.1781838580892945 2312 76-77 -0.023624228156677418 2312 78-79 0.2647309783831737 2312 80-81 0.09934924539259526 2312 82-83 0.3532962104591917 2312 84-85 0.40570137065228096 2312 86-87 0.30717892277865744 2312 88-89 0.627112921194481 2312 90-91 0.865460890834882 2312 92-93 1.6303618271224032 2312 94-95 0.8413696488022033 2312 96-97 0.8446037737909649 2312 98-99 0.646186547976944 2312 100-101 0.6584165433264904 2313 1 -0.19188861184537132 2313 2 -0.6325818977604136 2313 3 -0.3064487529734947 2313 4 0.06955110568072342 2313 5 -0.6346955007121906 2313 6 -0.09079449160001474 2313 7 -1.0297082083878735 2313 8 -0.6710568361069704 2313 9 -1.2151991217107394 2313 10-11 -1.9654172143410733 2313 12-13 -0.24185256543125178 2313 14-15 0.15991700986020163 2313 16-17 -1.1426584595350349 2313 18-19 -0.9253533073579021 2313 20-21 -1.209006871580577 2313 22-23 -0.7537285261679827 2313 24-25 -0.16176728619817027 2313 26-27 -0.7204712528604134 2313 28-29 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2.129137035620449 2313 94-95 0.7365331660800081 2313 96-97 0.6108281925531109 2313 98-99 0.1925427801339925 2313 100-101 0.0074873712393461744 2314 1 -0.6563307678763124 2314 2 -0.552783191793484 2314 3 -2.201372792757347 2314 4 -0.7422797762436133 2314 5 -0.7611756832671404 2314 6 -2.9711309747402375 2314 7 -4.276050524823646 2314 8 -1.8514669370778165 2314 9 -2.003123638607221 2314 10-11 -1.4402840653761473 2314 12-13 -2.738484711596108 2314 14-15 -3.394646540030454 2314 16-17 -2.342163859975024 2314 18-19 -2.979860726351305 2314 20-21 -2.794795931668709 2314 22-23 -3.4904044544326993 2314 24-25 -2.0196249641493083 2314 26-27 -2.152354250163974 2314 28-29 -1.8151708510649982 2314 30-31 -1.6744429940107821 2314 32-33 -2.571227983333685 2314 34-35 -1.2118697286737259 2314 36-37 -0.724915570799439 2314 38-39 -1.284445939227048 2314 40-41 -1.3543126154791238 2314 42-43 -1.0685106196044174 2314 44-45 -1.561952450337106 2314 46-47 -2.0328125662262977 2314 48-49 -2.108383944746212 2314 50-51 -1.2794594479873354 2314 52-53 -0.8866322407442802 2314 54-55 -0.4664068300035389 2314 56-57 -0.5965876815947908 2314 58-59 -0.8404938496816001 2314 60-61 -0.744154896991688 2314 62-63 -0.8548078795227241 2314 64-65 -1.043127913661845 2314 66-67 -1.227544287619434 2314 68-69 -0.9026349021579101 2314 70-71 -0.9481937968080096 2314 72-73 -0.8131149113577685 2314 74-75 -0.46299085144027075 2314 76-77 -0.4541999582774068 2314 78-79 -0.38099203715784213 2314 80-81 -0.29932462876156407 2314 82-83 -0.35704036084806035 2314 84-85 -0.6547836107123786 2314 86-87 -0.7416555537605731 2314 88-89 -0.39378220586849366 2314 90-91 0.21321813318261817 2314 92-93 1.1876804820143647 2314 94-95 0.35324981083403273 2314 96-97 -0.15107425626522186 2314 98-99 -0.027637680700673073 2314 100-101 0.05941168951880549 2315 1 -6.987142105016762 2315 2 -3.031848430352394 2315 3 -1.463362143354292 2315 4 -0.8584231835262628 2315 5 -1.3732071402353085 2315 6 -1.5362116347576062 2315 7 -2.916543257088531 2315 8 -2.5078899131210477 2315 9 -3.1991134453565166 2315 10-11 -2.0443504004551443 2315 12-13 -2.921073946400533 2315 14-15 -4.343618881407856 2315 16-17 -3.7021130253039303 2315 18-19 -2.5746592736814975 2315 20-21 -3.9853108136392805 2315 22-23 -5.494481316104498 2315 24-25 -3.6637570465725275 2315 26-27 -4.917276791985252 2315 28-29 -2.9510340755358158 2315 30-31 -2.470081256421203 2315 32-33 -1.7518336133064025 2315 34-35 -2.158823376576734 2315 36-37 -2.61934152060514 2315 38-39 -2.0939058372945425 2315 40-41 -3.3892576140317168 2315 42-43 -7.109261692001059 2315 44-45 -3.780071128898822 2315 46-47 -2.462631991298892 2315 48-49 -5.171497447733131 2315 50-51 -6.077522116303172 2315 52-53 -5.967554326413204 2315 54-55 -3.9808041387046487 2315 56-57 -3.170598401175127 2315 58-59 -3.4780588733225706 2315 60-61 -2.2651521600775624 2315 62-63 -2.4680466051032184 2315 64-65 -1.1692299163461293 2315 66-67 -1.531131224117761 2315 68-69 -1.2093140252927306 2315 70-71 -0.8361327337975801 2315 72-73 -1.1899866711409217 2315 74-75 -1.73701560647768 2315 76-77 -1.6776236010048464 2315 78-79 -0.6921219353130574 2315 80-81 -0.4939854531692234 2315 82-83 -0.7792477434984804 2315 84-85 -1.0689293777062439 2315 86-87 -1.3748662961485287 2315 88-89 -0.9465566209846799 2315 90-91 0.10369112808599468 2315 92-93 0.7027466679666219 2315 94-95 0.6028002375996522 2315 96-97 0.6838742511516394 2315 98-99 0.6306186769227704 2315 100-101 0.35270668851000586 2316 1 -4.160072971618515 2316 2 -2.6989370379473314 2316 3 -4.109302700687255 2316 4 -4.25573388969773 2316 5 -3.7113093334007914 2316 6 -3.848855952465218 2316 7 -2.370377819150942 2316 8 -1.0561001417110205 2316 9 -1.6373083727919493 2316 10-11 -2.636913112005189 2316 12-13 -3.7141455372569325 2316 14-15 -2.681816127200044 2316 16-17 -2.5052764230875866 2316 18-19 -2.962293158783737 2316 20-21 -5.35081018768296 2316 22-23 -6.374232788261033 2316 24-25 -4.2647690092933495 2316 26-27 -3.6407860885958137 2316 28-29 -4.441663277557424 2316 30-31 -6.265399334967128 2316 32-33 -4.4092291713348715 2316 34-35 -6.014854581658575 2316 36-37 -9.516094661978531 2316 38-39 -6.177941632722742 2316 40-41 -5.451773262939771 2316 42-43 -4.548641299735095 2316 44-45 -3.4707733591580165 2316 46-47 -5.992732376146108 2316 48-49 -3.0561267424890097 2316 50-51 -4.231523600051489 2316 52-53 -4.918040022152059 2316 54-55 -6.5178769814736945 2316 56-57 -4.649290384297494 2316 58-59 -5.55944246863022 2316 60-61 -5.981680884517676 2316 62-63 -6.2446207693356115 2316 64-65 -5.143231863765795 2316 66-67 -6.9236684337435825 2316 68-69 -5.637725487248499 2316 70-71 -6.4890758876901025 2316 72-73 -8.275930474173332 2316 74-75 -7.434523372972791 2316 76-77 -6.721381425458873 2316 78-79 -6.234481790647596 2316 80-81 -6.414159793596728 2316 82-83 -5.217019570827269 2316 84-85 -6.66889112481989 2316 86-87 -5.225231437336458 2316 88-89 -5.008661920748208 2316 90-91 -5.209249939285453 2316 92-93 -4.0874605931267105 2316 94-95 -3.92235161476739 2316 96-97 -4.23736769255866 2316 98-99 -5.335062688125683 2316 100-101 -4.418432088324973 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4734.0 3 1772.0 4 1559.0 5 1705.0 6 2338.0 7 2776.0 8 3783.0 9 4813.0 10 6114.0 11 7668.0 12 10023.0 13 11712.0 14 13096.0 15 14987.0 16 16984.0 17 19399.0 18 15977.0 19 13074.0 20 13464.0 21 14542.0 22 15817.0 23 17608.0 24 19134.0 25 21983.0 26 24708.0 27 27392.0 28 31231.0 29 35389.0 30 40166.0 31 45374.0 32 51800.0 33 59930.0 34 72229.0 35 79713.0 36 77677.0 37 62181.0 38 18236.0 39 331.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 50.457203005401496 18.16900784733632 14.177891881396329 17.19589726586585 2 14.133930518206265 23.80021713060542 41.10071135256923 20.965140998619088 3 21.64545031285132 32.13227194785433 31.013615561940018 15.208662177354334 4 10.90718310594894 16.935273236020233 40.096684737801986 32.060858920228846 5 10.811547397611212 42.257220530220955 32.45973831894565 14.471493753222184 6 23.81291917257165 38.484342375217565 19.851081971903586 17.85165648030719 7 23.588719292707466 39.68141858209921 20.676678922709712 16.05318320248361 8 23.29689969660084 31.38046618098577 21.60447286666788 23.718161255745507 9 28.1116445106526 19.462396846231055 21.97589710283812 30.450061540278227 10-11 22.63695348055558 27.05095246314145 27.303148533379357 23.008945522923614 12-13 30.65837686971787 25.672065140499228 26.597315032344287 17.07224295743862 14-15 26.043708903314304 25.687312880732527 31.16270724709765 17.10627096885552 16-17 17.805762237444608 29.848946371845713 32.148958578773005 20.19633281193667 18-19 17.04145687272463 30.41909959506079 35.64271325170958 16.896730280505004 20-21 19.879209524231346 27.87567302985805 33.23044110782901 19.014676338081593 22-23 18.60861970468079 27.579159336309772 32.22502501502037 21.58719594398907 24-25 19.468894346884664 27.686882709387472 34.42610007520523 18.418122868522637 26-27 21.3661711568164 25.466922743521536 35.662507973999226 17.504398125662842 28-29 18.29549405031049 26.339106171908195 37.30939294307975 18.05600683470156 30-31 17.604704903952335 30.421455416342873 35.157205051648056 16.816634628056732 32-33 17.505325282452983 29.6468556692247 33.60199264969014 19.245826398632186 34-35 18.93147964701499 26.989516781329236 33.915166359183154 20.16383721247262 36-37 20.545766447405793 27.18482669302341 34.978812355861535 17.290594503709258 38-39 22.115257910903505 25.139724458823316 35.79627037932351 16.948747250949673 40-41 18.27672028652794 27.339654426222804 37.25113110607009 17.132494181179165 42-43 16.954916875913355 30.213536694040542 34.55435709251136 18.27718933753474 44-45 17.68937507347383 30.876273759378385 32.072356745195876 19.36199442195191 46-47 18.087020140426894 29.17320130940892 30.106578342721825 22.633200207442364 48-49 19.53088782030888 28.669292161692923 31.142681186426813 20.65713883157139 50-51 21.804838941530548 29.770973282583707 30.983918868781085 17.440268907104663 52-53 18.675874280051456 30.093508075697446 33.02063587309813 18.209981771152965 54-55 16.607449993494026 29.404842603730508 33.83657561901926 20.151131783756203 56-57 19.038257152091905 32.26026156124445 29.367443958262445 19.3340373284012 58-59 18.192837190175588 29.77716611552692 30.6838487465026 21.34614794779489 60-61 18.49686425477632 28.080624036586155 32.03719501597691 21.38531669266062 62-63 22.92876169272623 27.4070119440453 30.59629354067393 19.067932822554535 64-65 21.14844045470601 28.350015502889896 32.54516565377333 17.95637838863076 66-67 18.084477413074456 29.035610777569087 33.07518297797903 19.80472883137742 68-69 17.751786446519056 31.90807595365178 31.662329519445603 18.677808080383564 70-71 18.29784333660875 31.12638376907741 29.326305386262558 21.24946750805128 72-73 18.169830444534842 29.762370094970148 28.728754434326515 23.339045026168492 74-75 18.874247822307076 30.43178835040826 29.99340867895153 20.700555148333137 76-77 20.451245741691505 28.20248949451499 31.056458280652006 20.2898064831415 78-79 18.26414897068692 28.43852049847062 32.64992786370303 20.64740266713943 80-81 17.570911859889588 31.99894303468381 30.763316484396974 19.66682862102963 82-83 17.641222462848603 30.857123049235717 29.48892433566187 22.012730152253805 84-85 18.479304510658796 28.51838090335893 29.55789930585146 23.444415280130805 86-87 19.49009593014172 28.958574200274413 30.49743343550353 21.05389643408034 88-89 20.979957608096512 26.65433550975412 31.252947828409393 21.11275905373998 90-91 18.121382926988804 28.0373459677901 32.63108201495318 21.21018909026792 92-93 17.15534193201053 30.18966060014326 31.275977388551013 21.379020079295195 94-95 17.34958408959434 30.63388405424849 29.479574986049183 22.53695687010798 96-97 17.205151724913982 28.915766728841092 28.44133155562224 25.437749990622688 98-99 18.49084424819608 28.358899559009608 29.05602590235911 24.094230290435203 100-101 20.10712277791579 28.6015088371744 29.495373494332057 21.795994890577756 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 11150.0 1 7660.0 2 4237.5 3 4091.0 4 3278.5 5 3187.0 6 4816.0 7 8188.0 8 11678.5 9 11950.5 10 8913.5 11 6082.5 12 5089.0 13 4738.0 14 4546.0 15 4241.0 16 3945.5 17 3840.5 18 3815.0 19 3905.0 20 4026.5 21 4163.0 22 4336.5 23 4736.0 24 5203.0 25 5847.0 26 6818.5 27 7718.5 28 8654.5 29 9530.0 30 10316.0 31 11353.5 32 12658.5 33 14028.0 34 15518.0 35 16522.0 36 17563.0 37 19275.0 38 19349.0 39 18192.0 40 17635.5 41 17740.5 42 17930.0 43 18240.0 44 19522.0 45 21180.5 46 25986.0 47 39610.0 48 80441.5 49 82201.5 50 42334.0 51 26416.0 52 18679.5 53 15418.0 54 13321.0 55 11790.5 56 10925.0 57 10300.5 58 9932.5 59 9659.5 60 9368.0 61 8670.0 62 7259.0 63 5142.5 64 3225.5 65 2200.0 66 1595.0 67 1155.0 68 838.5 69 626.5 70 474.0 71 428.5 72 387.5 73 306.5 74 242.5 75 194.0 76 156.0 77 99.5 78 61.5 79 38.0 80 21.0 81 16.5 82 14.5 83 12.0 84 11.0 85 6.5 86 3.5 87 3.0 88 3.0 89 1.5 90 0.0 91 0.5 92 0.5 93 1.0 94 1.0 95 0.0 96 0.5 97 0.5 98 0.5 99 1.0 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08372862395750488 2 0.09564123305714989 3 0.09178381677726484 4 0.09359907149721075 5 0.09133000309727836 6 0.0755599777177483 7 0.0867918662974136 8 0.08293445001752855 9 0.07885012689765027 10-11 0.08565733209744741 12-13 0.08372862395750488 14-15 0.09047910244730373 16-17 0.08752931352739163 18-19 0.08996856205731894 20-21 0.1005197301170045 22-23 0.1075538421567949 24-25 0.13171942061607475 26-27 0.1377891785858939 28-29 0.13671137109592602 30-31 0.1396611600158381 32-33 0.134328849275997 34-35 0.13103870009609503 36-37 0.1348961163759801 38-39 0.12650056329623027 40-41 0.12321041411632833 42-43 0.1223595134663537 44-45 0.11385050696660726 46-47 0.13325104178602912 48-49 0.1510065020155 50-51 0.16025295574522447 52-53 0.16961286289494554 54-55 0.16547181306506895 56-57 0.1366546443859277 58-59 0.1272947372362066 60-61 0.12321041411632833 62-63 0.12071443887640271 64-65 0.1064193079568287 66-67 0.1195231779664382 68-69 0.11799155679648385 70-71 0.12752164407619984 72-73 0.15350247725542562 74-75 0.16723034107501655 76-77 0.17261937852485593 78-79 0.1687619622449709 80-81 0.17488844692478833 82-83 0.1746615400847951 84-85 0.15293521015544254 86-87 0.1533322971254307 88-89 0.1740375462748137 90-91 0.2656511829220836 92-93 0.5326638068841266 94-95 0.17494517363478662 96-97 0.18560979511446885 98-99 0.20512378335388728 100-101 0.21187426184368616 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 881419.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 39.792563973621945 #Duplication Level Percentage of deduplicated Percentage of total 1 77.84045617838777 30.974713322144105 2 10.708432168892209 8.52231944275669 3 4.309356831506786 5.144410722086957 4 2.1438525797921506 3.412375637255744 5 1.2594036205905474 2.505744957048023 6 0.8195187767928352 1.956645201186798 7 0.5413107873802857 1.507810089550918 8 0.37773437090929884 1.2024815295555291 9 0.2877380506092288 1.0304851307861704 >10 1.5052554381534613 10.868583950950155 >50 0.11696678768137994 3.267209549744426 >100 0.07197952744329872 5.507444481581572 >500 0.006965760720311996 1.8854387275462146 >1k 0.007256000750324997 6.547698485805668 >5k 0.0026121602701169987 8.004423672362508 >10k+ 0.0011609601200519995 7.6622150996385 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 22383 2.539427899784325 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 17250 1.9570714949416792 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15945 1.8090147818460915 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10765 1.2213260662636045 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 9432 1.070092657408111 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 8792 0.9974824686102749 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8733 0.9907887168304745 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8241 0.934969634192138 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 7723 0.8762007626338892 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 7521 0.8532831717945721 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 7127 0.8085825243159043 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 6118 0.6941080235393156 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 5619 0.6374947669610027 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 4405 0.4997623150851071 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 4132 0.4687895314260301 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 3661 0.4153529706076225 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 3472 0.39391027422826147 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3194 0.36237022346920134 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 3190 0.3619164097892149 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 2847 0.32300188673037455 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2826 0.32061936491044557 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2576 0.2922560099112908 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 2351 0.2667289904120515 No Hit GCTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 2343 0.26582136305207854 No Hit CCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 2221 0.251980045812491 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2122 0.24074815723282572 No Hit GTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGC 2102 0.23847908883289334 No Hit GATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTT 1903 0.21590185825356611 No Hit TGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGT 1617 0.18345418013453307 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1613 0.1830003664545466 No Hit AAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTAC 1584 0.17971021727464465 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1446 0.1640536453151112 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1422 0.16133076323519235 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1162 0.13183287403607138 No Hit ATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGT 1157 0.1312656069360883 No Hit GCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAAC 1138 0.12910999195615253 No Hit GTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTT 1123 0.12740819065620324 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1086 0.12321041411632833 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 999 0.11333996657662247 No Hit TACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGA 930 0.10551168059685576 No Hit GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG 921 0.10449059981688619 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 911 0.10335606561691998 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 886 0.1005197301170045 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 2.269068399932382E-4 0.0 12-13 0.0 0.0 0.0 9.076273599729527E-4 0.0 14-15 0.0 0.0 0.0 0.001134534199966191 0.0 16-17 0.0 0.0 0.0 0.0015316211699543578 0.0 18-19 0.0 0.0 0.0 0.002212341689934072 0.0 20-21 0.0 0.0 0.0 0.0032901491799019536 0.0 22-23 0.0 0.0 0.0 0.007658105849771789 0.0 24-25 0.0 0.0 0.0 0.012309696069633173 0.0 26-27 0.0 0.0 0.0 0.017074739709491173 0.0 28-29 0.0 0.0 0.0 0.0297247960391142 0.0 30-31 0.0 0.0 0.0 0.06591643701803569 0.0 32-33 0.0 0.0 0.0 0.12139515939638243 0.0 34-35 0.0 0.0 0.0 0.21028591396373347 0.0 36-37 0.0 0.0 0.0 0.34745109873964597 0.0 38-39 0.0 0.0 0.0 0.5057753463449279 0.0 40-41 0.0 0.0 0.0 0.6884353525394846 0.0 42-43 0.0 0.0 0.0 0.8680888431041309 0.0 44-45 0.0 0.0 0.0 1.0466645261788092 0.0 46-47 0.0 0.0 0.0 1.282988000031767 0.0 48-49 0.0 0.0 0.0 1.564295754913384 0.0 50-51 0.0 0.0 0.0 1.8324996397853914 0.0 52-53 0.0 0.0 0.0 2.065192604198457 0.0 54-55 0.0 0.0 0.0 2.290567823021741 0.0 56-57 0.0 0.0 0.0 2.5302948994745975 0.0 58-59 0.0 0.0 0.0 2.8779729050542366 0.0 60-61 0.0 0.0 0.0 3.3894209223989953 0.0 62-63 0.0 0.0 0.0 3.824968601766016 0.0 64-65 0.0 0.0 0.0 4.135660792426757 0.0 66-67 0.0 0.0 0.0 4.4829984377464065 0.0 68-69 0.0 0.0 0.0 4.8691371527049 0.0 70-71 0.0 0.0 0.0 5.330665665251146 0.0 72-73 0.0 0.0 0.0 5.886984510204568 0.0 74-75 0.0 0.0 0.0 6.4124440249189085 0.0 76-77 0.0 0.0 0.0 6.858486145635617 0.0 78-79 0.0 0.0 0.0 7.359156088080697 0.0 80-81 0.0 0.0 0.0 7.931755498803634 0.0 82-83 0.0 0.0 0.0 8.506226890956514 0.0 84-85 0.0 0.0 0.0 9.247474810504425 0.0 86-87 0.0 0.0 0.0 10.074380062149784 0.0 88-89 0.0 0.0 0.0 10.695197176371284 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTCACT 140 0.0 44.063904 1 CATATAG 140 4.6020432E-10 33.89531 3 GAGTGCG 95 3.5160927E-5 29.967188 7 TTGCGTT 390 0.0 29.202112 4 CTTTGCG 400 0.0 28.47206 2 TTTGCGT 405 0.0 28.120554 3 ATGGGAG 1845 0.0 26.74882 5 TATATGG 235 5.456968E-12 26.250837 2 ATATGGG 235 5.456968E-12 26.250837 3 GTCGGGA 500 0.0 25.624857 2 TACATGG 9260 0.0 25.520317 2 GTACATG 9345 0.0 25.440525 1 GTATATG 245 1.0913936E-11 25.179373 1 CATGGGG 1170 0.0 25.146267 4 AGTGCGC 95 0.0012337667 24.974073 8 ATGGGAT 2325 0.0 24.900297 5 ACATGGG 9165 0.0 24.179766 3 CATGGGT 435 0.0 23.999437 4 CGTATAC 60 1.6491546E-4 23.879951 90-91 CGTAGCT 120 1.7224472E-4 23.72537 8 >>END_MODULE