##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939641_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 881419 Sequences flagged as poor quality 0 Sequence length 101 %GC 39 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.470611593351176 31.0 31.0 34.0 30.0 34.0 2 31.609927854970223 31.0 31.0 34.0 30.0 34.0 3 31.615264703846865 31.0 31.0 34.0 30.0 34.0 4 35.38404663389375 37.0 35.0 37.0 33.0 37.0 5 35.18195546045638 37.0 35.0 37.0 32.0 37.0 6 35.26379848857354 37.0 35.0 37.0 32.0 37.0 7 35.207766113505606 37.0 35.0 37.0 32.0 37.0 8 35.20889724410298 37.0 35.0 37.0 32.0 37.0 9 36.70977934444345 39.0 35.0 39.0 32.0 39.0 10-11 36.610600633750806 39.0 35.0 39.0 32.0 39.0 12-13 36.54889558768305 38.5 35.0 39.0 32.0 39.0 14-15 37.252277293772885 39.0 36.0 41.0 32.0 41.0 16-17 37.26745055416323 39.0 36.0 40.0 32.0 41.0 18-19 37.47295383920701 39.0 36.0 41.0 32.0 41.0 20-21 37.45936211949141 39.0 36.0 41.0 32.0 41.0 22-23 37.31788740655693 39.0 36.0 41.0 32.0 41.0 24-25 37.141217173671095 39.0 36.0 40.5 31.0 41.0 26-27 36.65259598442965 38.5 35.0 40.0 30.5 41.0 28-29 36.365760778925804 38.0 35.0 40.0 30.0 41.0 30-31 36.08979554559183 38.0 35.0 40.0 30.0 41.0 32-33 35.76229069262179 38.0 35.0 40.0 30.0 41.0 34-35 35.62123689187548 38.0 34.0 40.0 30.0 41.0 36-37 35.2950991526164 38.0 34.0 40.0 28.0 41.0 38-39 35.06774133527868 38.0 34.0 40.0 26.5 41.0 40-41 34.81822776681692 38.0 33.5 40.0 25.5 41.0 42-43 34.635052114828476 38.0 33.0 40.0 25.0 41.0 44-45 34.29481665360061 38.0 33.0 40.0 24.0 41.0 46-47 33.70007794249953 37.0 33.0 40.0 21.5 41.0 48-49 33.21103357200151 37.0 33.0 40.0 18.5 41.0 50-51 32.923352571251584 37.0 33.0 40.0 15.0 41.0 52-53 32.447209556408474 36.0 31.5 40.0 10.0 41.0 54-55 32.202621568175864 36.0 31.0 40.0 7.5 41.0 56-57 30.502840873636714 35.5 27.5 39.5 2.0 40.5 58-59 25.988471430727046 32.0 13.0 37.5 2.0 40.0 60-61 26.61004017385602 32.0 17.5 37.0 2.0 40.0 62-63 29.30377777197905 34.0 27.0 38.0 2.0 40.0 64-65 29.279039821015886 35.0 28.0 38.0 2.0 40.0 66-67 23.117266022175606 26.0 15.0 34.5 2.0 37.5 68-69 16.32520571941381 13.0 6.5 28.0 2.0 34.5 70-71 15.32015647495686 12.0 6.5 25.5 2.0 33.5 72-73 16.405335033621924 13.0 6.5 28.0 2.0 34.0 74-75 17.280880602755328 16.0 6.5 29.0 2.0 34.0 76-77 18.22039631548673 19.5 7.0 30.0 2.0 33.5 78-79 21.047181873773994 25.5 7.5 33.0 2.0 35.0 80-81 24.39090546039965 30.5 15.5 34.0 2.0 35.5 82-83 25.542818455240926 32.5 20.0 35.0 2.0 36.0 84-85 25.579855891465918 33.0 20.0 35.0 2.0 35.0 86-87 25.28329772786836 33.0 18.0 35.0 2.0 35.0 88-89 24.73595815384057 32.5 7.0 35.0 2.0 35.0 90-91 23.467723636545163 31.5 2.0 34.5 2.0 35.0 92-93 21.453549333517884 30.5 2.0 34.0 2.0 35.0 94-95 14.982450457727822 3.5 2.0 31.5 2.0 34.5 96-97 12.637472643544104 2.0 2.0 29.0 2.0 34.0 98-99 12.378745522844413 2.0 2.0 29.0 2.0 34.0 100-101 12.156471553256736 2.0 2.0 29.5 2.0 34.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.5284778463928497 1101 2 0.6726851489459769 1101 3 0.6880369939604023 1101 4 0.45158005832480086 1101 5 0.6880348250561781 1101 6 0.5983884445268615 1101 7 0.650162895570837 1101 8 0.7919154153813892 1101 9 0.7508102601886364 1101 10-11 0.846315896962544 1101 12-13 0.8745315106954905 1101 14-15 0.9583865441513879 1101 16-17 0.9476314887706963 1101 18-19 0.6656722283711858 1101 20-21 0.6439837291372115 1101 22-23 0.9153738728679102 1101 24-25 0.8090506811690972 1101 26-27 0.9462346122316276 1101 28-29 0.9380861932562681 1101 30-31 0.9694120673559183 1101 32-33 1.0582256990856607 1101 34-35 1.2713902983034444 1101 36-37 1.4670044447757675 1101 38-39 1.3830345918148268 1101 40-41 1.5854206424049622 1101 42-43 1.43107591419259 1101 44-45 1.2583730705850158 1101 46-47 1.6883439188075897 1101 48-49 2.0930227934068597 1101 50-51 1.6423573079572762 1101 52-53 1.9326457670364334 1101 54-55 1.6287838333460272 1101 56-57 -1.1950569649772973 1101 58-59 -8.765008171984327 1101 60-61 -8.671041094664279 1101 62-63 -1.9996062931613032 1101 64-65 -3.196119039695251 1101 66-67 -8.35383341673447 1101 68-69 -4.135680648225348 1101 70-71 -3.5105083427039254 1101 72-73 -3.698805990456165 1101 74-75 -5.551828924695872 1101 76-77 -6.10230537299638 1101 78-79 -9.29660281209641 1101 80-81 -6.212528955113044 1101 82-83 -2.398517118371558 1101 84-85 -1.6692204165590425 1101 86-87 -1.4788376548967648 1101 88-89 -1.676378806528966 1101 90-91 -7.188195682345313 1101 92-93 -14.9197413865114 1101 94-95 -9.687049514923167 1101 96-97 -8.263541345318405 1101 98-99 -8.411732167151929 1101 100-101 -8.538541203751134 1102 1 0.4007420847012284 1102 2 0.30534965478535625 1102 3 0.46903972071864786 1102 4 0.18584975014967142 1102 5 0.1723431030826248 1102 6 0.3116363737340464 1102 7 0.4639021557447336 1102 8 0.46850388067952764 1102 9 0.5693290645308693 1102 10-11 0.5947441523517512 1102 12-13 0.49254483579709785 1102 14-15 0.42280363526626985 1102 16-17 0.3730520783678628 1102 18-19 0.47377790311523427 1102 20-21 0.423578059537995 1102 22-23 0.36725044847114674 1102 24-25 0.3492240618348319 1102 26-27 0.39115538189772536 1102 28-29 0.1642502103370873 1102 30-31 0.42014134656567137 1102 32-33 0.6837774817681748 1102 34-35 0.38651480311385455 1102 36-37 0.5083971494112589 1102 38-39 0.5160643803624367 1102 40-41 0.4844264046110638 1102 42-43 0.8875571098503556 1102 44-45 0.7923765176190258 1102 46-47 0.7157787081269262 1102 48-49 0.641758452201941 1102 50-51 0.5743105414809762 1102 52-53 0.4573319325969578 1102 54-55 0.3420317625532121 1102 56-57 -2.712723014264739 1102 58-59 -9.741456440750083 1102 60-61 -10.244834632761723 1102 62-63 -4.315928256427238 1102 64-65 -5.2678829435898855 1102 66-67 -6.391213156406231 1102 68-69 -4.1837307588391255 1102 70-71 -3.9171798969561262 1102 72-73 -4.401233062535191 1102 74-75 -5.315057560802268 1102 76-77 -7.361998226831847 1102 78-79 -9.602335435443635 1102 80-81 -5.701293939421319 1102 82-83 -3.487848033636258 1102 84-85 -3.122457798871128 1102 86-87 -2.9816029993429254 1102 88-89 -2.834674839867443 1102 90-91 -8.22513039449225 1102 92-93 -12.033013753646248 1102 94-95 -9.497420199699626 1102 96-97 -8.554262650046502 1102 98-99 -8.822794831143284 1102 100-101 -8.777811667300035 1103 1 0.17638691476861368 1103 2 0.24111875021127815 1103 3 0.17107465787692888 1103 4 0.2662230714381195 1103 5 0.2969839888015571 1103 6 0.1369505823170698 1103 7 0.1724838709848342 1103 8 0.16461704303857516 1103 9 0.29982528562446475 1103 10-11 0.4190210655708313 1103 12-13 0.3138549529720862 1103 14-15 0.44389815256941034 1103 16-17 0.4956892770926373 1103 18-19 0.5019516330192815 1103 20-21 0.2649237336224175 1103 22-23 0.49672132279296477 1103 24-25 0.6111516454881141 1103 26-27 0.506521665334084 1103 28-29 0.49306890161173555 1103 30-31 0.5534777874083261 1103 32-33 0.6843118858354202 1103 34-35 0.8679189859089362 1103 36-37 0.6308702191227553 1103 38-39 0.7054771398832358 1103 40-41 0.5507417476538308 1103 42-43 0.5850246648270101 1103 44-45 0.3460906915380022 1103 46-47 0.4158677317037274 1103 48-49 0.17811302864404155 1103 50-51 0.32857592627815535 1103 52-53 0.4686666822542662 1103 54-55 0.38194531251933483 1103 56-57 -2.318116818854051 1103 58-59 -9.576758228049385 1103 60-61 -10.686778261667335 1103 62-63 -4.590778702070335 1103 64-65 -5.226193517700043 1103 66-67 -5.920836473755585 1103 68-69 -4.023636693219032 1103 70-71 -4.01783102957762 1103 72-73 -4.298204028769257 1103 74-75 -4.856026132065772 1103 76-77 -6.102153112099103 1103 78-79 -8.699629144969421 1103 80-81 -4.935408220483723 1103 82-83 -2.82182365850824 1103 84-85 -2.4320333481124585 1103 86-87 -2.277876876831776 1103 88-89 -2.2819487226084227 1103 90-91 -4.455562277012643 1103 92-93 -8.752362988673742 1103 94-95 -7.71913289251839 1103 96-97 -7.614603040489728 1103 98-99 -7.766308846447986 1103 100-101 -7.825887024838678 1104 1 0.0576511233186352 1104 2 0.11707083553156039 1104 3 0.15445687761983962 1104 4 0.033811681804436944 1104 5 0.0303581107956461 1104 6 -0.05174376065558306 1104 7 0.084359969429471 1104 8 0.0983846556422705 1104 9 0.3313849120483212 1104 10-11 0.18980258830750785 1104 12-13 0.29517314651427995 1104 14-15 0.29203716992258677 1104 16-17 0.14902405002576558 1104 18-19 0.2494374319671735 1104 20-21 0.11623826818181016 1104 22-23 0.13562770681820524 1104 24-25 0.16071646646788196 1104 26-27 0.19957499650802646 1104 28-29 0.32999523609074544 1104 30-31 0.3105543201322263 1104 32-33 0.340651223499556 1104 34-35 0.3458835117707437 1104 36-37 0.37417877934301913 1104 38-39 0.26958491792094463 1104 40-41 0.2034886840055279 1104 42-43 0.10405317490121035 1104 44-45 0.34405965616583245 1104 46-47 0.3703463866376637 1104 48-49 0.24782624836896616 1104 50-51 0.45568359034754735 1104 52-53 0.23927365351624985 1104 54-55 0.19559151887396808 1104 56-57 -2.2278791049385234 1104 58-59 -9.49141395375764 1104 60-61 -9.564342698753826 1104 62-63 -3.607450467319598 1104 64-65 -4.79111287012007 1104 66-67 -5.851365586662197 1104 68-69 -3.7428373554349363 1104 70-71 -3.939646364914397 1104 72-73 -3.8080250985118695 1104 74-75 -4.7533448774545555 1104 76-77 -6.369571369823454 1104 78-79 -8.803036797641976 1104 80-81 -4.776586772846166 1104 82-83 -2.692735345059379 1104 84-85 -2.167140388355268 1104 86-87 -2.1303976760495935 1104 88-89 -2.2485452087852664 1104 90-91 -4.14848124553939 1104 92-93 -5.800545194020657 1104 94-95 -7.2608728887994225 1104 96-97 -7.298672669132966 1104 98-99 -7.257334141415986 1104 100-101 -7.419362638618047 1105 1 0.22204783487211088 1105 2 0.1553368386541223 1105 3 0.2592663526393544 1105 4 0.1657337641619705 1105 5 0.011671537805796106 1105 6 0.17289641303684533 1105 7 0.21503424733734988 1105 8 0.24610128327692848 1105 9 0.3751166157953918 1105 10-11 0.29779439158620136 1105 12-13 0.3171774400349676 1105 14-15 0.5237787292512408 1105 16-17 0.44322840697346066 1105 18-19 0.32805582092111507 1105 20-21 0.30892708551201764 1105 22-23 0.3033647042811296 1105 24-25 0.4290151588956732 1105 26-27 0.4676943856571256 1105 28-29 0.5655259494006657 1105 30-31 0.6225694694296493 1105 32-33 0.5736094836478784 1105 34-35 0.749179276798067 1105 36-37 0.38368551236408877 1105 38-39 0.5632117231512339 1105 40-41 0.48937129524674816 1105 42-43 0.3959436657286872 1105 44-45 0.5762458144406253 1105 46-47 0.35944486926841535 1105 48-49 0.3331593621472564 1105 50-51 0.5400567607222584 1105 52-53 0.3476899298628524 1105 54-55 0.39246590899887224 1105 56-57 1.3672870250380598 1105 58-59 -8.666978698098 1105 60-61 -9.431875388059055 1105 62-63 -3.611476252838724 1105 64-65 -4.750602526638417 1105 66-67 -6.1566031940002155 1105 68-69 -3.9872167465356405 1105 70-71 -3.7731559521509315 1105 72-73 -2.5799299823916506 1105 74-75 -4.276171282606779 1105 76-77 -5.873967959401909 1105 78-79 -8.085714515245412 1105 80-81 -4.129555644821185 1105 82-83 -1.826912135652666 1105 84-85 -1.6195768393701009 1105 86-87 -1.5113177336218016 1105 88-89 -1.604622614132655 1105 90-91 -3.5807540060780347 1105 92-93 -5.492792461780219 1105 94-95 -6.7933847203763165 1105 96-97 -7.318186842630233 1105 98-99 -7.358434356092021 1105 100-101 -7.363567605440842 1106 1 0.24274089693300027 1106 2 0.280849133736087 1106 3 0.19645531633959123 1106 4 0.29649708383410456 1106 5 0.28406615138893443 1106 6 0.22311962732256063 1106 7 0.2641413901944958 1106 8 0.2604455455720114 1106 9 0.32061207832466465 1106 10-11 0.36790563280400335 1106 12-13 0.33225355244714194 1106 14-15 0.4732627749186875 1106 16-17 0.4670958689172551 1106 18-19 0.4167286107337631 1106 20-21 0.3590954110389575 1106 22-23 0.4211468325012646 1106 24-25 0.42597798090972105 1106 26-27 0.48041028144167086 1106 28-29 0.5878016373397656 1106 30-31 0.49029670889100885 1106 32-33 0.5019705053106378 1106 34-35 0.7939319114584862 1106 36-37 0.6059118004202944 1106 38-39 0.6833632161254641 1106 40-41 0.5586409088298794 1106 42-43 0.663498920412529 1106 44-45 0.6537395937146258 1106 46-47 0.47826988390541914 1106 48-49 0.6598481062924577 1106 50-51 0.6565032624787008 1106 52-53 0.6683134661626227 1106 54-55 0.7295103323009826 1106 56-57 1.5954230027898149 1106 58-59 -8.320063446341557 1106 60-61 -9.498729896255778 1106 62-63 -3.485241255766123 1106 64-65 -4.717888826404224 1106 66-67 -5.928375734983824 1106 68-69 -3.496584428034467 1106 70-71 -3.355571423462406 1106 72-73 -3.946705816700785 1106 74-75 -4.274305064808184 1106 76-77 -5.784325468184111 1106 78-79 -7.957759670397637 1106 80-81 -3.9820512537204813 1106 82-83 -1.8411740648095751 1106 84-85 -1.4715510050609701 1106 86-87 -1.343824686197916 1106 88-89 -1.4482719847415524 1106 90-91 -3.972485929380138 1106 92-93 -5.996524897377409 1106 94-95 -6.79057624555196 1106 96-97 -6.744535276470982 1106 98-99 -6.280675281150568 1106 100-101 -6.8085507728881485 1107 1 -0.008585043279150995 1107 2 -0.06971568235428194 1107 3 -0.12394005492366844 1107 4 -0.07015121482962172 1107 5 -0.1621730000093322 1107 6 -0.09725852393794554 1107 7 -0.022623315687859247 1107 8 -0.12049535537853018 1107 9 -0.07439756487687532 1107 10-11 0.03466413948536484 1107 12-13 -0.17704524904066687 1107 14-15 -0.10154952705128295 1107 16-17 -0.2031410783206553 1107 18-19 -0.1614071517679676 1107 20-21 -0.19803194810142344 1107 22-23 -0.0498719028617316 1107 24-25 0.08303301603852731 1107 26-27 0.30087453271594455 1107 28-29 0.2645477456191472 1107 30-31 0.03147834548006756 1107 32-33 -0.037833531058005576 1107 34-35 -0.2688091685800842 1107 36-37 -0.48593108427456144 1107 38-39 -0.5579148524790227 1107 40-41 -0.35083353668603223 1107 42-43 -0.6073717229267714 1107 44-45 -0.9094750363997122 1107 46-47 -0.9761315487988043 1107 48-49 -1.2074459424745925 1107 50-51 -1.1123526405382478 1107 52-53 -1.4713751961875658 1107 54-55 -1.5148673367994476 1107 56-57 -0.11794144134849205 1107 58-59 -9.526944559442533 1107 60-61 -10.885000388059055 1107 62-63 -5.130285338973177 1107 64-65 -6.41473067789892 1107 66-67 -7.443236597361562 1107 68-69 -4.490893217123876 1107 70-71 -4.202941929041687 1107 72-73 -2.7616854970975346 1107 74-75 -4.9349160305059385 1107 76-77 -6.936894692595187 1107 78-79 -7.819465828270623 1107 80-81 -3.7678171994430336 1107 82-83 -2.885308931871151 1107 84-85 -2.5410251061768214 1107 86-87 -2.5651189416049966 1107 88-89 -2.339424347325931 1107 90-91 -2.0605662434729908 1107 92-93 -3.8097567474945038 1107 94-95 -5.448354520796483 1107 96-97 -5.838177651453762 1107 98-99 -5.8330929695373985 1107 100-101 -6.7304925003988245 1108 1 -0.15038955222709305 1108 2 -0.2934297201295628 1108 3 -0.23298230195947056 1108 4 -0.055416553841943994 1108 5 -0.05353808134218241 1108 6 -0.33588985634445834 1108 7 -0.23480486902573716 1108 8 -0.24657898166361036 1108 9 -0.1481915187070939 1108 10-11 -0.23664976015246708 1108 12-13 -0.2289707610789904 1108 14-15 -0.3380318702520029 1108 16-17 -0.34876539759525826 1108 18-19 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1.5311872403002305 2316 86-87 1.4066166757188583 2316 88-89 1.3596545149882253 2316 90-91 2.307355540625263 2316 92-93 3.9413421536043955 2316 94-95 10.188337463689619 2316 96-97 11.615183693084052 2316 98-99 11.115775808743145 2316 100-101 10.541566323130587 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 6.0 4 38.0 5 164.0 6 375.0 7 588.0 8 972.0 9 1611.0 10 2737.0 11 4220.0 12 5911.0 13 7940.0 14 9661.0 15 12435.0 16 16350.0 17 21213.0 18 20802.0 19 17301.0 20 17281.0 21 17020.0 22 16528.0 23 14932.0 24 14820.0 25 17345.0 26 23682.0 27 34401.0 28 47265.0 29 60778.0 30 72730.0 31 78211.0 32 77498.0 33 71809.0 34 64546.0 35 55618.0 36 41437.0 37 24214.0 38 8444.0 39 535.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 48.202534461021344 19.05793491471415 15.151387916428833 17.588142707835676 2 14.289928542415034 24.43127795389409 40.076041399561134 21.202752104129743 3 21.437137161781173 31.514296832720873 31.81630983675187 15.232256168746078 4 11.04968238715072 17.50949321491822 39.68101436433751 31.759810033593556 5 10.604077626224413 42.55195871931726 32.86958458018355 13.97437907427478 6 24.10873829586156 38.617388551869205 19.399854098901884 17.874019053367356 7 23.014593513414162 39.46341070478399 20.745525113481783 16.776470668320062 8 23.3871187332937 32.01494433880491 21.737137566881927 22.860799361019467 9 27.02449118977467 19.367633327622844 22.17617273963915 31.431702742963335 10-11 22.52714089439869 27.154792442640783 27.4026881653334 22.915378497627124 12-13 29.982165122376532 25.780020625831757 26.94405271499707 17.293761536794648 14-15 25.440598855929224 25.830767380595272 31.280987141116434 17.447646622359073 16-17 17.634121796784502 29.87983013753958 32.41591116143401 20.07013690424191 18-19 17.05249447905729 30.094455912726843 35.78183526176331 17.071214346452557 20-21 19.45470882747025 27.823033086420875 33.43143272382375 19.290825362285133 22-23 18.278553077407874 27.643149002939023 32.35687368783508 21.721424231818023 24-25 19.3356394631838 27.71111128759421 34.56783890521988 18.385410344002114 26-27 21.08177130989868 25.626960270156356 35.7201002251492 17.571168194795757 28-29 18.238077139080662 26.27380459385406 37.39668366623364 18.091434600831636 30-31 17.6295014537976 30.271271541025456 35.308701510531165 16.79052549464577 32-33 17.333535285805294 29.563629494364335 33.87174089270935 19.231094327121028 34-35 18.59964975359649 26.935105706452216 34.206377615998555 20.258866923952734 36-37 20.348207288957536 27.109913572815753 35.0787611725023 17.463117965724408 38-39 21.903928514237755 25.079584468564274 35.958153844756055 17.058333172441912 40-41 18.22817015790804 27.162397385331396 37.33620696998867 17.2732254867719 42-43 16.993931752806247 30.00019877272043 34.867915527273034 18.137953947200288 44-45 17.619107925043487 30.723379074214265 32.29919908499308 19.35831391574917 46-47 17.90107787736355 29.16668796185719 30.233889336561116 22.69834482421814 48-49 19.31725516473782 28.41730172971868 31.41523469886086 20.850208406682643 50-51 21.547878369384737 29.579092381391526 31.18289339490427 17.690135854319465 52-53 18.577746741154563 29.861527456056685 33.287402915928595 18.273322886860154 54-55 16.51409478114459 29.167324222541886 34.10075307650999 20.21782791980354 56-57 18.486611675850817 31.78571836031534 30.236106832780003 19.491563131053837 58-59 17.87588838485437 29.376386975543117 31.60050493114837 21.147219708454138 60-61 18.526077834596208 27.946512737954503 32.152692355288366 21.374717072160927 62-63 22.651842808711873 27.505241827514375 30.747119264056494 19.09579609971726 64-65 20.76689764852891 28.285186868113144 32.97801885720777 17.969896626150174 66-67 19.156960922728647 28.69136862123393 32.32615695340375 19.825513502633665 68-69 20.1966526634454 30.962016043239988 29.807232251924788 19.03409904138983 70-71 19.96573118001577 30.50812046143853 28.586743825980694 20.93940453256501 72-73 18.87334663639288 29.414195265913552 28.759143910819766 22.953314186873797 74-75 19.844725423736666 29.78228672471273 29.58722368965002 20.785764161900584 76-77 18.571500096970546 28.72641702169781 32.01570404934046 20.68637883199119 78-79 17.475244434543427 28.517621106270685 33.061099902472435 20.946034556713453 80-81 17.227090122948074 31.96281022225098 31.282514947084994 19.527584707715953 82-83 17.43355592919591 31.300018833289084 29.565257346684547 21.701167890830458 84-85 18.23599218986657 28.75403185091313 29.611002258857592 23.39897370036271 86-87 19.16985991315126 29.211639206165067 30.550959108481507 21.067541772202166 88-89 20.5944402056914 26.93887970229772 31.506975979033776 20.959704112977107 90-91 17.684444583455363 28.443093511065108 32.773542683776384 21.098919221703145 92-93 15.847770099178845 30.71115571203849 32.222929711393775 21.218144477388886 94-95 16.952802993503386 31.001321787139585 29.567330377887536 22.478544841469493 96-97 17.22027819546249 29.72790990569823 27.982356067398612 25.069455831440667 98-99 17.327401643684247 29.101095789497915 29.48485232164645 24.08665024517139 100-101 19.127716829525156 29.483065790325156 29.759952903420515 21.62926447672917 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 6834.0 1 4885.5 2 2866.5 3 2814.5 4 2887.5 5 3329.0 6 4288.5 7 5710.5 8 6946.5 9 7428.0 10 7224.0 11 6925.5 12 6896.5 13 6813.0 14 6510.5 15 6137.5 16 5959.0 17 5800.5 18 5528.0 19 5306.5 20 5108.5 21 5039.5 22 5226.0 23 5501.0 24 5844.0 25 6332.0 26 7088.0 27 7964.5 28 8887.5 29 9919.0 30 10901.5 31 11910.5 32 13309.5 33 14735.0 34 16226.0 35 17647.5 36 18949.5 37 20026.5 38 20156.5 39 20005.0 40 20030.0 41 20205.0 42 21140.0 43 22988.0 44 26451.0 45 31964.0 46 39309.5 47 48699.0 48 59279.0 49 54929.0 50 39216.0 51 28288.0 52 20591.0 53 16258.0 54 13475.0 55 11675.0 56 10644.5 57 9865.5 58 9236.0 59 8413.0 60 7316.5 61 6213.0 62 4908.0 63 3531.0 64 2364.5 65 1575.5 66 1118.5 67 783.0 68 557.0 69 427.5 70 343.0 71 292.0 72 257.0 73 205.5 74 154.5 75 108.5 76 60.0 77 36.5 78 22.5 79 12.0 80 8.0 81 5.5 82 5.5 83 4.0 84 1.5 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.023371404519303533 2 0.006466844939807289 3 0.0 4 0.0 5 0.1362575574159395 6 0.0 7 0.0 8 0.0017018012999492863 9 0.0 10-11 0.0 12-13 0.0 14-15 5.672670999830954E-4 16-17 0.0 18-19 2.836335499915477E-4 20-21 0.0 22-23 3.970869699881668E-4 24-25 0.0 26-27 3.970869699881668E-4 28-29 0.002722882079918858 30-31 0.012082789229639932 32-33 0.0026661553699205485 34-35 0.0044814100898664545 36-37 0.10312915877692674 38-39 0.10301570535693014 40-41 0.113623600126614 42-43 0.11532540142656329 44-45 0.11214870566665797 46-47 0.10647603466682701 48-49 0.10539822717685914 50-51 0.1040367861368997 52-53 0.0838420773775015 54-55 0.07170256143786327 56-57 0.5569428387634031 58-59 1.5853980910327552 60-61 0.5275016762742805 62-63 0.005332310739841097 64-65 0.12695437697621675 66-67 1.921844208032729 68-69 2.8981108871036363 70-71 2.798158424086615 72-73 2.6628652207406467 74-75 2.025370453779644 76-77 1.4293429118274055 78-79 0.9453506221218285 80-81 0.05423073475838392 82-83 1.134534199966191E-4 84-85 0.0 86-87 1.701801299949286E-4 88-89 1.701801299949286E-4 90-91 0.2486331699225907 92-93 0.398221504188133 94-95 2.0212294039497674 96-97 2.4415175983272426 98-99 1.4339377753372686 100-101 0.9438757276618726 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 881419.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 33.07973674212647 #Duplication Level Percentage of deduplicated Percentage of total 1 71.99807462772374 23.816773546250765 2 13.129906319062876 8.686676889667654 3 5.406072369871667 5.364943524127154 4 2.8560083746048286 3.7790402066094497 5 1.6373200668999475 2.708105838782558 6 1.054976068964491 2.0938998378353286 7 0.7303842144354138 1.6912642273889833 8 0.535221317963013 1.4163984237592346 9 0.41254009497710953 1.2282045963673172 >10 1.9664011296795874 11.715509475762834 >50 0.15027110109095254 3.4518444388234926 >100 0.09880838726586463 6.320909554050752 >500 0.009263286306174612 2.031157523814436 >1k 0.010292540340194013 8.104882572815788 >5k 0.0020585080680388026 5.58026397096899 >10k+ 0.00240159274604527 12.010125372975297 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 23255 2.638359282021377 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 19484 2.2105264352141263 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16749 1.9002313315233732 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 12568 1.4258825825175088 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12306 1.3961577864783945 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10789 1.2240489483435233 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 10673 1.2108883516239155 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9478 1.0753115147279557 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 9409 1.067483228748189 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 8795 0.9978228288702649 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 7989 0.9063793723529899 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 7672 0.8704146382140617 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 5826 0.6609796249003028 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 4995 0.5666998328831123 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 4776 0.5418535339038527 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 4449 0.5047542655649584 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 4221 0.4788868858057292 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 3861 0.4380436546069463 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3614 0.4100206598677814 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 3264 0.37031196286896473 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3185 0.3613491426892318 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2987 0.33888536552990123 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 2904 0.3294687316701818 No Hit GCTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 2728 0.30950092975077687 No Hit CCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 2574 0.29202910307129754 No Hit GATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTT 2278 0.25844689075229826 No Hit GTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGC 2266 0.2570854497123388 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2249 0.2551567415723963 No Hit TGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGT 2075 0.2354158464929846 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1901 0.21567495141357287 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1777 0.20160672733399213 No Hit AAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTAC 1712 0.19423225503421188 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1642 0.18629051563444854 No Hit GCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAAC 1448 0.16428055215510443 No Hit ATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGT 1420 0.16110385639519909 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1302 0.14771635283559806 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1278 0.1449934707556792 No Hit GTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTT 1148 0.13024452615611873 No Hit TACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGA 1148 0.13024452615611873 No Hit GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG 1111 0.12604674961624382 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 1060 0.12026062519641624 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1038 0.11776464995649062 No Hit AGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAG 1003 0.11379378025660894 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 999 0.11333996657662247 No Hit GCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAA 903 0.10244843825694705 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 1.7018012999492864E-4 0.0 12-13 0.0 0.0 0.0 9.076273599729527E-4 0.0 14-15 0.0 0.0 0.0 0.001134534199966191 0.0 16-17 0.0 0.0 0.0 0.0015316211699543578 0.0 18-19 0.0 0.0 0.0 0.002212341689934072 0.0 20-21 0.0 0.0 0.0 0.0032901491799019536 0.0 22-23 0.0 0.0 0.0 0.008055192819759955 0.0 24-25 0.0 0.0 0.0 0.013160596719607814 0.0 26-27 0.0 0.0 0.0 0.018379454039452292 0.0 28-29 0.0 0.0 0.0 0.03165350417905673 0.0 30-31 0.0 0.0 0.0 0.06880949922794947 0.0 32-33 0.0 0.0 0.0 0.12508239554627254 0.0 34-35 0.0 0.0 0.0 0.21771711297351204 0.0 36-37 0.0 0.0 0.0 0.35913680099929773 0.0 38-39 0.0 0.0 0.0 0.5208646512044782 0.0 40-41 0.0 0.0 0.0 0.7106154961488236 0.0 42-43 0.0 0.0 0.0 0.8968492850732739 0.0 44-45 0.0 0.0 0.0 1.082685987027736 0.0 46-47 0.0 0.0 0.0 1.314868411050817 0.0 48-49 0.0 0.0 0.0 1.55000062399381 0.0 50-51 0.0 5.672670999830955E-5 0.0 1.7524582519777767 0.0 52-53 0.0 1.134534199966191E-4 0.0 1.9296724940124959 0.0 54-55 0.0 1.134534199966191E-4 0.0 2.100816978077396 0.0 56-57 0.0 1.134534199966191E-4 0.0 2.2315153179135008 1.134534199966191E-4 58-59 0.0 1.134534199966191E-4 0.0 2.3444581975201353 1.134534199966191E-4 60-61 0.0 1.134534199966191E-4 0.0 2.466307170596504 1.134534199966191E-4 62-63 0.0 1.134534199966191E-4 0.0 2.561267683133674 1.134534199966191E-4 64-65 0.0 1.134534199966191E-4 0.0 2.622589256641847 1.134534199966191E-4 66-67 0.0 1.134534199966191E-4 0.0 2.676876718110229 1.134534199966191E-4 68-69 0.0 1.134534199966191E-4 0.0 2.7480687391581076 1.134534199966191E-4 70-71 0.0 1.134534199966191E-4 0.0 2.8791074392542026 1.134534199966191E-4 72-73 0.0 1.134534199966191E-4 0.0 3.081791974078163 1.134534199966191E-4 74-75 0.0 1.134534199966191E-4 0.0 3.318852895161098 1.134534199966191E-4 76-77 0.0 1.134534199966191E-4 0.0 3.623532054562019 1.134534199966191E-4 78-79 0.0 1.134534199966191E-4 0.0 4.085741287628245 1.134534199966191E-4 80-81 0.0 1.134534199966191E-4 0.0 4.678308500270587 1.134534199966191E-4 82-83 0.0 1.134534199966191E-4 0.0 5.268833551352989 1.134534199966191E-4 84-85 0.0 1.134534199966191E-4 0.0 5.867470521965149 1.134534199966191E-4 86-87 0.0 1.134534199966191E-4 0.0 6.331495009751322 1.134534199966191E-4 88-89 0.0 1.134534199966191E-4 0.0 6.639067231362156 1.134534199966191E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCGGGA 425 0.0 33.21224 2 CGGGAGT 460 0.0 30.68522 4 TCGGGAG 465 0.0 30.355272 3 TTACCGC 45 7.7470386E-4 27.048882 68-69 ATGGGAG 1855 0.0 25.617886 5 ATGGGAT 2390 0.0 25.198687 5 GTACATG 9145 0.0 25.161718 1 TACATGG 9050 0.0 24.799082 2 GTAGCAA 660 0.0 24.718655 64-65 CGGCTTG 455 0.0 24.527166 74-75 ACTGCGC 110 1.6552804E-9 24.313265 62-63 CCGCTTG 170 0.0 24.260569 74-75 ACATGGG 9015 0.0 23.851532 3 CGGCGTT 245 0.0 23.765453 74-75 GCAACTC 360 0.0 23.66777 68-69 GGGAGTG 660 0.0 23.525335 5 GGTGGCG 160 2.0419538E-6 23.494638 9 GGTAGCA 730 0.0 23.349045 64-65 CATGGGA 7365 0.0 23.189943 4 GGTCGGG 610 0.0 23.142303 1 >>END_MODULE