FastQCFastQC Report
Fri 27 May 2016
SRR939639_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939639_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4023126
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74560.18532852314344617No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51130.12709022784769852No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA96800.038.8255771
GGTATCA58400.038.5965841
ATCAACG118850.031.1432193
TCAACGC120500.030.7561594
TATCAAC122400.030.5501172
CAACGCA121950.030.3126375
AACGCAG126000.029.3719446
GTACATG120200.028.1088831
TACATGG121650.027.0326862
ACATGGG125100.025.2630043
ACGCAGA144200.025.138357
CGCAGAG145050.024.6975638
GCAGAGT155800.021.1357149
CATGGGG65550.019.6908444
GAGTACT77500.018.97938512-13
CATGGGA75800.018.906274
AGTACAT85950.018.440332
AGAGTAC139700.018.06902510-11
GAGTACA101200.017.8185021
GTACTTT86250.017.33029714-15
Produced by FastQC (version 0.11.5)