##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939639_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4023126 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.986130436879183 31.0 30.0 33.0 26.0 34.0 2 30.150706937838883 31.0 30.0 34.0 25.0 34.0 3 30.191756609164116 31.0 30.0 34.0 26.0 34.0 4 33.76679577025428 35.0 35.0 37.0 28.0 37.0 5 33.749583284242156 35.0 33.0 37.0 28.0 37.0 6 33.8339492722823 35.0 35.0 37.0 28.0 37.0 7 33.62861267581478 35.0 33.0 37.0 28.0 37.0 8 33.618270469281846 35.0 33.0 37.0 28.0 37.0 9 34.87564048453864 37.0 34.0 39.0 28.0 39.0 10-11 34.75342370584466 37.0 34.0 39.0 27.0 39.0 12-13 34.57542741639213 37.0 34.0 39.0 27.0 39.0 14-15 35.268646197011975 38.0 33.5 40.0 27.0 41.0 16-17 34.97941078653763 38.0 33.0 40.0 25.5 41.0 18-19 34.90642400958856 38.0 33.0 40.0 25.5 41.0 20-21 35.02694571335822 38.0 34.0 40.0 25.0 41.0 22-23 34.871186858179435 38.0 33.0 40.0 25.0 41.0 24-25 34.74527531576192 38.0 33.0 40.0 25.0 41.0 26-27 34.89295612416812 38.0 34.0 40.0 25.0 41.0 28-29 34.773134870744784 38.0 33.0 40.0 24.0 41.0 30-31 34.56429229410165 38.0 33.0 40.0 24.0 41.0 32-33 34.35642221993544 38.0 33.0 40.0 24.0 41.0 34-35 34.13243408235287 38.0 33.0 40.0 23.5 41.0 36-37 33.857599911113894 38.0 32.5 40.0 21.5 41.0 38-39 33.70911960003241 38.0 32.0 40.0 21.0 41.0 40-41 33.439713421851565 38.0 31.5 40.0 18.5 41.0 42-43 33.13892629761037 37.0 31.0 40.0 17.5 41.0 44-45 32.914368080940044 37.0 31.0 40.0 16.5 41.0 46-47 32.50835842576146 37.0 30.5 40.0 14.5 41.0 48-49 32.18016661670552 37.0 30.0 39.5 11.0 41.0 50-51 31.584328455037202 36.0 30.0 38.5 9.0 40.0 52-53 31.640366222683554 36.0 30.0 39.0 9.0 40.0 54-55 31.664840847639375 36.0 30.0 39.0 8.0 40.0 56-57 31.514999903060456 36.0 30.0 39.0 8.0 40.0 58-59 31.091066996161693 36.0 29.0 39.0 6.5 40.0 60-61 30.66018526389678 35.0 28.0 39.0 2.0 40.0 62-63 30.229234431136383 35.0 28.0 38.0 2.0 40.0 64-65 29.587300397750404 34.0 27.0 38.0 2.0 40.0 66-67 29.026357489176327 34.0 26.0 37.5 2.0 39.5 68-69 28.490315615270315 34.0 26.0 37.0 2.0 39.0 70-71 27.922343471221133 33.0 25.5 36.0 2.0 39.0 72-73 27.153055236152184 32.0 23.5 36.0 2.0 38.5 74-75 26.670793557049915 32.0 23.0 35.0 2.0 38.0 76-77 26.2533298733373 32.0 22.0 35.0 2.0 37.0 78-79 25.659019379457664 31.0 20.5 35.0 2.0 36.5 80-81 24.969520715980558 31.0 18.5 34.0 2.0 36.0 82-83 24.28226993139166 30.5 15.0 34.0 2.0 35.5 84-85 23.59249822153221 30.0 7.5 34.0 2.0 35.0 86-87 22.893537885713748 29.5 4.5 34.0 2.0 35.0 88-89 22.073345080417567 29.0 2.0 34.0 2.0 35.0 90-91 20.797723213242637 28.0 2.0 33.5 2.0 34.5 92-93 19.13474323200417 25.0 2.0 32.0 2.0 34.0 94-95 18.8213728578225 25.0 2.0 32.0 2.0 34.0 96-97 18.155328717022535 24.5 2.0 32.0 2.0 34.0 98-99 17.133725242510426 21.0 2.0 32.0 2.0 34.0 100-101 15.533184270142174 8.5 2.0 30.5 2.0 34.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.9409473853248507 1101 2 0.9622923645347292 1101 3 0.9430051788383658 1101 4 1.0795777987579669 1101 5 0.9883198268347897 1101 6 0.9085058849636454 1101 7 0.9485678362688006 1101 8 0.8994319017720187 1101 9 1.244469423118737 1101 10-11 0.9957722496067731 1101 12-13 0.9592375898155652 1101 14-15 1.159565220318484 1101 16-17 0.9066639401729972 1101 18-19 1.0468496314962081 1101 20-21 1.2522596909733963 1101 22-23 1.261675606355162 1101 24-25 1.1381869061935745 1101 26-27 1.065426421869482 1101 28-29 1.155843056722425 1101 30-31 1.3351946758319642 1101 32-33 1.0661588162218436 1101 34-35 1.2182404640760396 1101 36-37 1.0838946783446985 1101 38-39 0.9592714653417929 1101 40-41 1.1128247307121129 1101 42-43 1.065414438904675 1101 44-45 0.9638289511586962 1101 46-47 1.0328437526750989 1101 48-49 0.9843215167163208 1101 50-51 0.9126558569631591 1101 52-53 1.0360223231526966 1101 54-55 1.188878154533203 1101 56-57 1.091311001508526 1101 58-59 0.9386133531853993 1101 60-61 0.8191874393641072 1101 62-63 0.9123803750949406 1101 64-65 0.8089616975187326 1101 66-67 0.6470381145844541 1101 68-69 0.46392210301213765 1101 70-71 0.3977531590700991 1101 72-73 0.42783189961993173 1101 74-75 0.17493678889974618 1101 76-77 -0.2969931366836853 1101 78-79 -0.14913736449398485 1101 80-81 -0.24538427321722622 1101 82-83 -0.34822239632899965 1101 84-85 -0.6554844762833589 1101 86-87 -1.0077790557597517 1101 88-89 -1.6700459330929895 1101 90-91 -5.029987385123693 1101 92-93 -9.73314417635419 1101 94-95 -8.192671442424924 1101 96-97 -5.066621500067281 1101 98-99 -3.8545918582187397 1101 100-101 -3.2148994551517767 1102 1 1.02938471427942 1102 2 0.8899036580942976 1102 3 0.9244524512339147 1102 4 1.045854481844998 1102 5 0.9031118355923198 1102 6 0.9462339455365338 1102 7 0.9211047820816134 1102 8 0.8604928395570965 1102 9 1.143367890551687 1102 10-11 0.8356098704788693 1102 12-13 0.8807494033532421 1102 14-15 1.096814353688302 1102 16-17 1.1472689848728734 1102 18-19 1.0760687520946348 1102 20-21 1.1685010246716274 1102 22-23 1.126949963224341 1102 24-25 1.2103977253871534 1102 26-27 1.1192635410083227 1102 28-29 1.1678379025534795 1102 30-31 1.1019229188533117 1102 32-33 1.0545676376047979 1102 34-35 0.9701059995622572 1102 36-37 0.9244860394101906 1102 38-39 0.899883351858854 1102 40-41 0.8415968525876778 1102 42-43 0.8068062925810153 1102 44-45 0.706139888943774 1102 46-47 0.7173299322043718 1102 48-49 0.6343363406535545 1102 50-51 0.5355246309095243 1102 52-53 0.6915413890165887 1102 54-55 0.7574502672863481 1102 56-57 0.7435630996657956 1102 58-59 0.7623213900399897 1102 60-61 0.5945625990065118 1102 62-63 0.5942153504643954 1102 64-65 0.5678412359241314 1102 66-67 0.5696605831120927 1102 68-69 0.5080290178087097 1102 70-71 0.5581356166508336 1102 72-73 0.5024145487715472 1102 74-75 0.4734646579017543 1102 76-77 0.38823880091894125 1102 78-79 0.1699524457596162 1102 80-81 -0.03124109678956799 1102 82-83 -0.17836630255049002 1102 84-85 -0.6740759741851967 1102 86-87 -1.0826992345592394 1102 88-89 -1.789127761226542 1102 90-91 -5.640912626867902 1102 92-93 -10.479986677722554 1102 94-95 -9.243258698400112 1102 96-97 -6.074105307766606 1102 98-99 -4.750213095219287 1102 100-101 -3.967047785748383 1103 1 0.9513099686992064 1103 2 0.879484892970936 1103 3 0.8729751377215891 1103 4 0.8357593132159096 1103 5 0.8860196887408733 1103 6 0.6349497479284807 1103 7 0.7212732375017055 1103 8 0.8185399144531829 1103 9 0.8365341577879803 1103 10-11 0.770209513885959 1103 12-13 0.6980207149899869 1103 14-15 0.7695260228441754 1103 16-17 0.9074437501351653 1103 18-19 0.9621766793404234 1103 20-21 0.9843711461216174 1103 22-23 0.87258748883999 1103 24-25 0.627058024323162 1103 26-27 0.6652976025784909 1103 28-29 0.8290491607012811 1103 30-31 0.813217246941953 1103 32-33 0.8660205800752507 1103 34-35 0.9478241522257136 1103 36-37 0.6838610755904782 1103 38-39 0.6655963061385464 1103 40-41 0.6500575405341422 1103 42-43 0.7126342133432857 1103 44-45 0.6652506643018086 1103 46-47 0.8263327455835565 1103 48-49 0.5973806369035444 1103 50-51 0.5728722395372223 1103 52-53 0.7769477081209395 1103 54-55 0.7541766748442633 1103 56-57 0.5353794080028926 1103 58-59 0.5525443185014289 1103 60-61 0.36668727917276556 1103 62-63 0.5770328976145507 1103 64-65 0.4476592924927374 1103 66-67 0.4940145478927569 1103 68-69 0.5496421376493998 1103 70-71 0.608163403111881 1103 72-73 0.5511025068139901 1103 74-75 0.2962206117299182 1103 76-77 0.028253041480230223 1103 78-79 0.14734758081339905 1103 80-81 -0.006017647331983511 1103 82-83 -0.21357232758145983 1103 84-85 -0.45747674757503276 1103 86-87 -0.754800469898985 1103 88-89 -1.1260125516124297 1103 90-91 -2.6032000314966623 1103 92-93 -9.089770116991769 1103 94-95 -5.785389630520104 1103 96-97 -3.3065561315194003 1103 98-99 -2.3673929430884115 1103 100-101 -1.9395426626196262 1104 1 0.9083101453631244 1104 2 0.7649445777395343 1104 3 0.7915892180109694 1104 4 0.8458030943835411 1104 5 0.8403620354324417 1104 6 0.8189205581512056 1104 7 0.9352363339829495 1104 8 0.9279398343422187 1104 9 1.0778031994474304 1104 10-11 0.866631719666195 1104 12-13 0.8418957243916552 1104 14-15 0.9545329407234391 1104 16-17 0.9236583495715891 1104 18-19 0.8179704688164904 1104 20-21 0.9221685668283754 1104 22-23 0.9162556615228468 1104 24-25 0.8412035569124043 1104 26-27 0.8315584669723108 1104 28-29 0.9344798271090795 1104 30-31 0.9012120589697901 1104 32-33 1.0122690954861113 1104 34-35 0.9831336726618716 1104 36-37 0.8660489795342841 1104 38-39 0.6563572203830788 1104 40-41 0.5877703899482682 1104 42-43 0.6698577561720427 1104 44-45 0.7897139820502446 1104 46-47 0.7482937325926358 1104 48-49 0.6609445778733019 1104 50-51 0.7639276228017202 1104 52-53 0.7970664927431308 1104 54-55 0.919206576524676 1104 56-57 0.8645101515480995 1104 58-59 0.603563634339789 1104 60-61 0.5195131147843668 1104 62-63 0.6276172004130096 1104 64-65 0.6002486433315397 1104 66-67 0.5173852605130342 1104 68-69 0.44851472448146623 1104 70-71 0.3471786679240978 1104 72-73 0.35526084316460427 1104 74-75 0.14289102157272282 1104 76-77 0.13411067484836892 1104 78-79 0.030833264913820102 1104 80-81 -0.07067716698103155 1104 82-83 0.04387685530417684 1104 84-85 -0.07739236868864552 1104 86-87 -0.3261635107573291 1104 88-89 -0.6297524043691745 1104 90-91 -1.1526480004737785 1104 92-93 -4.358338282153294 1104 94-95 -2.0288863867256417 1104 96-97 -1.2543030543312597 1104 98-99 -1.184491386871679 1104 100-101 -0.9666994929540458 1105 1 1.0099888024374657 1105 2 1.0292131628714287 1105 3 0.9632918559006569 1105 4 0.931894221481862 1105 5 0.7859255845931159 1105 6 0.7460188794941303 1105 7 0.9863154706735955 1105 8 1.0303379158769914 1105 9 1.2336784992076204 1105 10-11 1.0045214079155969 1105 12-13 1.0812242615619212 1105 14-15 1.1780341397642076 1105 16-17 1.1078310114420873 1105 18-19 1.0580184304471913 1105 20-21 1.0144947059171088 1105 22-23 1.0334019444964682 1105 24-25 1.0732179454016162 1105 26-27 0.946666380641382 1105 28-29 0.9180529685958803 1105 30-31 1.1561281261160659 1105 32-33 1.22210122977868 1105 34-35 1.1577140083932917 1105 36-37 1.123243224138605 1105 38-39 1.112592232373487 1105 40-41 0.9175066009794435 1105 42-43 1.2457570367475839 1105 44-45 1.1700497134651044 1105 46-47 1.0998524855902403 1105 48-49 1.0680189184008881 1105 50-51 0.9263976467825366 1105 52-53 0.9652919124073982 1105 54-55 1.1192065765246788 1105 56-57 1.1007211827231593 1105 58-59 1.0609976870975864 1105 60-61 0.9185538821704569 1105 62-63 1.0246195984945459 1105 64-65 1.2034860534034841 1105 66-67 1.1768576825753811 1105 68-69 0.9473156837140806 1105 70-71 1.0062913777562343 1105 72-73 1.1050210350111271 1105 74-75 1.0680708776878305 1105 76-77 0.6311130729299066 1105 78-79 0.48335125052532746 1105 80-81 0.5079799073594948 1105 82-83 0.6227737377981839 1105 84-85 0.5564205809516416 1105 86-87 0.23534728060957733 1105 88-89 0.010415461338261878 1105 90-91 -1.1984513577879277 1105 92-93 -7.221168018364324 1105 94-95 -2.9658168423611357 1105 96-97 -1.29219274258066 1105 98-99 -0.7203427058165239 1105 100-101 -0.5768913394768287 1106 1 0.893749368582867 1106 2 0.9149017351964304 1106 3 0.9098920661685561 1106 4 1.1306560995081298 1106 5 0.9228942040357424 1106 6 0.9082939561428347 1106 7 0.9625318961560083 1106 8 0.9060894034398146 1106 9 1.176285270714672 1106 10-11 0.9151892011484861 1106 12-13 0.9334284049037151 1106 14-15 1.0200173550282656 1106 16-17 1.0665753854914328 1106 18-19 1.0819803473711858 1106 20-21 0.9481172952213299 1106 22-23 1.1161187855607224 1106 24-25 0.8820525915332169 1106 26-27 1.0218701808758652 1106 28-29 1.0918401209703887 1106 30-31 1.0324207874728373 1106 32-33 1.056777452075515 1106 34-35 1.0692767245554862 1106 36-37 1.290522805217428 1106 38-39 1.1534515418654436 1106 40-41 1.1566441356342025 1106 42-43 1.1249844581769324 1106 44-45 1.0619570598145032 1106 46-47 1.1118272057376117 1106 48-49 1.0273737298845234 1106 50-51 0.9521946373461709 1106 52-53 1.0344236307511672 1106 54-55 1.2112005253594909 1106 56-57 1.1606641388402856 1106 58-59 1.1272294147988227 1106 60-61 0.9846800172226295 1106 62-63 1.1736994470869782 1106 64-65 1.2483741215258775 1106 66-67 1.1221414249770234 1106 68-69 1.1547896433375051 1106 70-71 1.0922115933817444 1106 72-73 1.1993155458446942 1106 74-75 1.0310439116554555 1106 76-77 0.7248894366897005 1106 78-79 0.7244941281130828 1106 80-81 0.9512681229996822 1106 82-83 1.0326855023421722 1106 84-85 0.8156823681553931 1106 86-87 0.691639997010288 1106 88-89 0.5407291751720926 1106 90-91 -0.1812859929107411 1106 92-93 -3.7231951036586004 1106 94-95 -1.3351498380915565 1106 96-97 -0.4997305531890994 1106 98-99 -0.10043321642257652 1106 100-101 0.09048612809769452 1107 1 0.6236756222674877 1107 2 0.658940725459523 1107 3 0.5699693130751129 1107 4 0.8095851902735802 1107 5 0.6710098267289055 1107 6 0.6284664919797294 1107 7 0.49677607758810893 1107 8 0.5128804894130994 1107 9 0.4934706272916358 1107 10-11 0.4223490278354163 1107 12-13 0.5441335806316161 1107 14-15 0.7021932791353009 1107 16-17 0.688311017215355 1107 18-19 0.4731624270244694 1107 20-21 0.46682949917636307 1107 22-23 0.565805112003062 1107 24-25 0.6567385662109189 1107 26-27 0.39196526453402925 1107 28-29 0.439928260337652 1107 30-31 0.5452949774928655 1107 32-33 0.34292221330711925 1107 34-35 0.36737804215314185 1107 36-37 0.41435958349917 1107 38-39 0.39756536206175497 1107 40-41 0.5485651646849661 1107 42-43 0.4160962275396898 1107 44-45 0.17262474443626985 1107 46-47 0.28520176527802477 1107 48-49 0.2927370278397703 1107 50-51 0.2443380973836078 1107 52-53 0.37580320721678717 1107 54-55 0.4235037358834042 1107 56-57 0.18396448060932968 1107 58-59 0.2444102882800685 1107 60-61 0.14289306379864541 1107 62-63 0.18639522695284683 1107 64-65 0.20118009783356783 1107 66-67 0.14874256778877637 1107 68-69 0.026318495317198654 1107 70-71 -0.03720161385477638 1107 72-73 0.0698664312043249 1107 74-75 0.024295207991915646 1107 76-77 0.08863856435906925 1107 78-79 -0.04791003612244893 1107 80-81 -0.2820920236316553 1107 82-83 -0.24031270253128056 1107 84-85 -0.3343578304725483 1107 86-87 -0.3702526721962691 1107 88-89 -0.4287312580667262 1107 90-91 -0.7670641818826063 1107 92-93 -4.1083584141250995 1107 94-95 -1.8458282857977402 1107 96-97 -1.116863915601641 1107 98-99 -0.9860506839766288 1107 100-101 -0.8133335303622857 1108 1 0.4186304625134163 1108 2 0.4693811667490344 1108 3 0.193933081569579 1108 4 -0.18204145643351666 1108 5 -0.7153383961559072 1108 6 -0.07682485059209654 1108 7 0.22291724709988614 1108 8 0.2393122344631493 1108 9 0.3707213148283657 1108 10-11 0.31328159036654313 1108 12-13 0.34238531658515114 1108 14-15 0.3727805505176036 1108 16-17 0.5293145943383948 1108 18-19 0.4413018843478156 1108 20-21 0.3625751353278943 1108 22-23 0.2837249863625857 1108 24-25 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0.47076302918632607 2311 60-61 0.41847421436107624 2311 62-63 0.5587407579428216 2311 64-65 0.6215014397521195 2311 66-67 0.4829848613692569 2311 68-69 0.5687583953166069 2311 70-71 0.6022178580485118 2311 72-73 0.698912364900238 2311 74-75 0.9165756979926591 2311 76-77 0.8857619712609051 2311 78-79 0.9314062293610519 2311 80-81 1.0354459881861082 2311 82-83 1.068412931141495 2311 84-85 1.0237286137200599 2311 86-87 1.1339905136741315 2311 88-89 1.2170190375377068 2311 90-91 2.070642360526012 2311 92-93 3.126583579015488 2311 94-95 2.246337729283564 2311 96-97 1.9374629959157907 2311 98-99 1.7443725018830882 2311 100-101 1.5731674054735194 2312 1 0.2247653506911469 2312 2 0.031008532659246413 2312 3 -0.01284570419217701 2312 4 -0.09614901156757583 2312 5 0.010249189900328304 2312 6 0.06419028734659804 2312 7 0.2051751402629094 2312 8 -0.6463085459738807 2312 9 0.006023700657735276 2312 10-11 0.04754004601338835 2312 12-13 0.052471607232682516 2312 14-15 -0.26905034876217115 2312 16-17 0.17550116071743815 2312 18-19 0.20981473887126612 2312 20-21 0.32269868176572203 2312 22-23 0.14942797851157508 2312 24-25 0.3216681228575027 2312 26-27 0.3903412327525331 2312 28-29 0.3317536251585338 2312 30-31 0.43641051617360915 2312 32-33 0.41809126843440225 2312 34-35 0.3462736951998764 2312 36-37 0.4783771353500512 2312 38-39 0.5114953688667541 2312 40-41 0.5592422842288656 2312 42-43 0.6212391337360614 2312 44-45 0.34474780781896897 2312 46-47 0.5280799863451691 2312 48-49 0.3851319736511556 2312 50-51 0.45893697430061664 2312 52-53 0.39052969578839125 2312 54-55 0.3860932327107953 2312 56-57 0.33846173508109345 2312 58-59 0.5699378755864686 2312 60-61 0.6200847174440831 2312 62-63 0.48844163413375696 2312 64-65 0.5894680573328834 2312 66-67 0.5197719711400559 2312 68-69 0.24934992301912473 2312 70-71 0.38424798808863514 2312 72-73 0.5554612554136469 2312 74-75 0.804497687928297 2312 76-77 1.0873595899189503 2312 78-79 1.1512477184102927 2312 80-81 1.065081441520224 2312 82-83 1.1554533512087772 2312 84-85 1.1231255976137788 2312 86-87 0.9712762381816127 2312 88-89 1.242573005265097 2312 90-91 1.8338371124870712 2312 92-93 2.8455492273872736 2312 94-95 2.4007943643120875 2312 96-97 1.8825496017679733 2312 98-99 1.9033260263862601 2312 100-101 1.9160301095415448 2313 1 -0.25430893331617455 2313 2 -0.7722619230101699 2313 3 -0.3251886558618615 2313 4 0.00990354001763194 2313 5 -0.8084096926518427 2313 6 -0.4051021310178129 2313 7 -1.2082133924682594 2313 8 -1.0794803678439138 2313 9 -1.1062174282411519 2313 10-11 -2.0445253184373726 2313 12-13 -0.5635920814684283 2313 14-15 -0.2059078134562924 2313 16-17 -1.4579780471538797 2313 18-19 -1.0420190949388157 2313 20-21 -1.323151872589058 2313 22-23 -1.0785352351084043 2313 24-25 -0.16426017150043037 2313 26-27 -0.7822143160101689 2313 28-29 -2.2967539367193552 2313 30-31 -0.5739821308764519 2313 32-33 -0.2755138950455702 2313 34-35 0.08747872583357719 2313 36-37 0.1299202465233762 2313 38-39 -0.3662829079440755 2313 40-41 -0.16047131219036004 2313 42-43 0.0016061653694734446 2313 44-45 -0.5712801507831813 2313 46-47 -0.7374964666784365 2313 48-49 -0.06030469537432026 2313 50-51 -0.14019066752236498 2313 52-53 -0.058604833191420624 2313 54-55 0.023957564183174185 2313 56-57 -0.20999811289474835 2313 58-59 -0.03566907620097837 2313 60-61 -0.12029938712814214 2313 62-63 -0.019629032150973558 2313 64-65 0.062438032183120384 2313 66-67 0.040982286183297134 2313 68-69 0.03411004943037099 2313 70-71 -0.1909028346374484 2313 72-73 0.06014131756278829 2313 74-75 0.15908797670170216 2313 76-77 0.10626144315246577 2313 78-79 0.23390591760429302 2313 80-81 0.19897848526010264 2313 82-83 0.1795673626699461 2313 84-85 0.39764584117328283 2313 86-87 0.35270253005676366 2313 88-89 0.7007317375315445 2313 90-91 1.385231299846879 2313 92-93 2.6390111049589073 2313 94-95 1.7399068258500314 2313 96-97 1.2483099526439538 2313 98-99 1.180225731307516 2313 100-101 1.0407480077425078 2314 1 -0.4844228377268891 2314 2 -0.5187509148459917 2314 3 -1.6798970180223876 2314 4 -0.44715054625319794 2314 5 -0.44454664809101274 2314 6 -2.7270596230986506 2314 7 -3.8071117219112907 2314 8 -1.4147643164453996 2314 9 -1.9188819369155325 2314 10-11 -1.1565431743139172 2314 12-13 -2.031163574398242 2314 14-15 -2.7563027385592136 2314 16-17 -2.111305072769511 2314 18-19 -2.5455454974699236 2314 20-21 -2.1197905721603476 2314 22-23 -3.122697576951147 2314 24-25 -1.8102782744813908 2314 26-27 -1.8959382401872489 2314 28-29 -1.080452379743015 2314 30-31 -1.3770773769171711 2314 32-33 -2.1795757782156286 2314 34-35 -0.7438231469755792 2314 36-37 -0.5157108122773408 2314 38-39 -0.5126663540138381 2314 40-41 -0.5946761107039222 2314 42-43 -0.5015804437560334 2314 44-45 -0.9110649156833901 2314 46-47 -1.3581383710589563 2314 48-49 -1.7054111042181113 2314 50-51 -0.7680640509400298 2314 52-53 -0.3905258823363944 2314 54-55 -0.028202399577491377 2314 56-57 -0.19040942654275383 2314 58-59 -0.34531770424506547 2314 60-61 -0.33225310311975065 2314 62-63 -0.3817385093403942 2314 64-65 -0.45689421381131723 2314 66-67 -0.3172466435466852 2314 68-69 -0.26794538161289694 2314 70-71 -0.28656762696545357 2314 72-73 0.00313672169868795 2314 74-75 0.1133747765385813 2314 76-77 0.028071690596551235 2314 78-79 0.15636836478423177 2314 80-81 0.2497326453643005 2314 82-83 0.32562084786523116 2314 84-85 0.21080686784049973 2314 86-87 0.4114239702782534 2314 88-89 0.5641947271891112 2314 90-91 1.3529764263379214 2314 92-93 2.2422281502376187 2314 94-95 1.4817799160794962 2314 96-97 1.3704319757458343 2314 98-99 1.3671289140481875 2314 100-101 1.3910883672635457 2315 1 -7.100862516507377 2315 2 -2.951782040965501 2315 3 -1.4543040673607344 2315 4 -0.9604169295972795 2315 5 -1.276949115646694 2315 6 -1.6869097775802118 2315 7 -2.8187654645782025 2315 8 -2.5538797100222865 2315 9 -3.2069240187779897 2315 10-11 -2.2406449589908775 2315 12-13 -2.7242538559440774 2315 14-15 -4.694560284696223 2315 16-17 -4.303747225967982 2315 18-19 -3.3123952386175546 2315 20-21 -4.260587728135654 2315 22-23 -5.438233247350055 2315 24-25 -3.658196922703901 2315 26-27 -4.399144470677562 2315 28-29 -2.8013115397973998 2315 30-31 -2.4907618618935246 2315 32-33 -1.655154465664971 2315 34-35 -2.3391385095923205 2315 36-37 -2.8465334545184717 2315 38-39 -2.0426760529982175 2315 40-41 -3.346394777917439 2315 42-43 -7.565289126321957 2315 44-45 -4.1981157541607885 2315 46-47 -2.625140020404963 2315 48-49 -5.3876090792010345 2315 50-51 -6.357355350819379 2315 52-53 -6.600985066009866 2315 54-55 -4.359645341940553 2315 56-57 -3.713292606245666 2315 58-59 -4.036326953190187 2315 60-61 -3.002796537493815 2315 62-63 -3.0425941305222395 2315 64-65 -1.7652189825677418 2315 66-67 -2.5810784685037547 2315 68-69 -1.9073755632883191 2315 70-71 -1.6502658764404075 2315 72-73 -2.0132868187059003 2315 74-75 -2.465794529986031 2315 76-77 -2.27600623162645 2315 78-79 -1.5834428142228738 2315 80-81 -1.3542802844870288 2315 82-83 -1.6566252430171637 2315 84-85 -1.6288986636526737 2315 86-87 -2.1163688464887436 2315 88-89 -1.5832371165993884 2315 90-91 -0.8055631683394857 2315 92-93 0.07804511113207724 2315 94-95 0.07431355332231959 2315 96-97 0.05220923583660664 2315 98-99 -0.03642484010909186 2315 100-101 0.04345488354475435 2316 1 -4.347890148795628 2316 2 -3.135951541627694 2316 3 -4.536185601911079 2316 4 -4.351797603966201 2316 5 -3.9226564859206867 2316 6 -4.501512605031479 2316 7 -3.193134678735092 2316 8 -1.7462648167380266 2316 9 -2.033293196331414 2316 10-11 -3.0989174875914998 2316 12-13 -3.9506565797409863 2316 14-15 -3.0056089365240695 2316 16-17 -2.8406868809969694 2316 18-19 -3.1676098181103 2316 20-21 -5.558176703671229 2316 22-23 -6.423670787697056 2316 24-25 -4.485762991957657 2316 26-27 -4.041806578271782 2316 28-29 -4.583536730967314 2316 30-31 -6.148512084010001 2316 32-33 -4.435322063778891 2316 34-35 -6.177168594312704 2316 36-37 -9.344001888189666 2316 38-39 -6.229960903656082 2316 40-41 -5.427587813775524 2316 42-43 -4.383523183849292 2316 44-45 -3.3870886276658716 2316 46-47 -5.752158930253959 2316 48-49 -3.148259751540131 2316 50-51 -4.394154311280985 2316 52-53 -5.287559996629664 2316 54-55 -6.810832334067481 2316 56-57 -4.791112196087205 2316 58-59 -5.61237013113627 2316 60-61 -6.2197037818186125 2316 62-63 -6.563512282679518 2316 64-65 -5.250768932986684 2316 66-67 -6.4983690925262785 2316 68-69 -5.276641505760985 2316 70-71 -6.162856116459309 2316 72-73 -7.803514338355306 2316 74-75 -7.508559751204064 2316 76-77 -6.801234129788956 2316 78-79 -5.8804692194631585 2316 80-81 -6.0682811929184695 2316 82-83 -4.872735738062385 2316 84-85 -6.09039525037959 2316 86-87 -4.745554639852202 2316 88-89 -4.530034916564784 2316 90-91 -5.202966672455712 2316 92-93 -3.825042328721775 2316 94-95 -3.6672931266436066 2316 96-97 -4.3401511011482015 2316 98-99 -5.29898003647004 2316 100-101 -4.438538395559991 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 23841.0 3 10059.0 4 10605.0 5 11712.0 6 13228.0 7 15464.0 8 18398.0 9 22311.0 10 26308.0 11 30477.0 12 34766.0 13 38860.0 14 41283.0 15 45130.0 16 49879.0 17 54371.0 18 54026.0 19 54790.0 20 58880.0 21 63982.0 22 70348.0 23 78496.0 24 87567.0 25 97611.0 26 110078.0 27 124585.0 28 140391.0 29 158412.0 30 180108.0 31 205720.0 32 234624.0 33 270716.0 34 315479.0 35 376777.0 36 427362.0 37 360566.0 38 104390.0 39 1526.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.16316175846844 18.735103570068354 12.850440030060906 24.251294641402303 2 15.458722660633976 20.043225002133386 40.34929802288034 24.148754314352296 3 19.22957698333996 24.549191727050587 29.250330508976358 26.970900780633094 4 12.586093825725497 16.11505747549666 37.42741547852792 33.871433220249926 5 13.820673616468557 37.399762711940305 34.72697568183 14.052587989761136 6 32.551076883660244 36.148541166842655 17.65579273487531 13.644589214621789 7 28.926308184770257 32.044372763655154 21.338054115347195 17.691264936227398 8 26.15916824177787 35.61762187479943 19.990760784930277 18.23244909849242 9 26.77064324250101 16.2594701304077 19.994761204455315 36.975125422635976 10-11 24.992241447400744 26.34351699076375 28.029153499931276 20.635088061904234 12-13 25.96908404172246 23.58078173521525 28.08150204450419 22.368632178558094 14-15 22.914797693095757 24.93398573882982 25.822646909408082 26.32856965866634 16-17 22.946365355632963 28.19392498603122 26.33554229965684 22.52416735867898 18-19 22.78536406181604 27.180160870381332 28.010087642426562 22.024387425376062 20-21 23.342248420805397 26.61156720470271 27.991699377229367 22.054484997262527 22-23 23.050628615165493 26.493791482803218 27.825508592790843 22.630071309240442 24-25 22.985596210558697 26.594495484084852 27.962871658609807 22.457036646746648 26-27 22.870151374014593 26.907314450627506 27.764148495500223 22.458385679857678 28-29 22.690370535565947 26.84216493355812 27.987115464143585 22.480349066732344 30-31 22.819217503657008 26.763571250605768 27.948586093937493 22.46862515179973 32-33 22.636436018825233 26.928661687655353 27.851007479468237 22.583894814051174 34-35 23.09558152190212 26.846684221929014 27.657290849117288 22.400443407051583 36-37 22.965926446941587 26.743646730475323 27.621006405630354 22.669420416952736 38-39 22.796674264749253 26.984491997374892 27.83801325943806 22.380820478437798 40-41 22.995294992474378 26.638204183290725 27.92441626649465 22.442084557740245 42-43 22.74468629426263 26.74535772448366 27.78198078385621 22.727975197397498 44-45 22.815960627956034 26.968222721389985 27.548100885637083 22.6677157650169 46-47 22.862460146992575 26.939849025234636 27.301867398062168 22.895823429710617 48-49 22.959021169062368 27.08529497023954 27.22449780746819 22.7311860532299 50-51 22.80347493567585 27.080523754812642 27.310161286507924 22.805840023003586 52-53 23.03971168569259 27.00603767008179 27.209301746567245 22.74494889765838 54-55 22.840381396062153 26.86901298905645 27.347998761144964 22.942606853736432 56-57 22.82501928610727 27.34238001826129 27.20322386307791 22.62937683255353 58-59 22.90830270427128 27.02626224566223 27.226319163018704 22.839115887047793 60-61 22.989283511858517 26.95263213692179 27.405274477178626 22.652809874041075 62-63 22.91784768816752 26.966235932882977 27.376746149102605 22.7391702298469 64-65 22.74565281652502 26.890580662883895 27.40487254797553 22.958893972615556 66-67 22.649281945811943 27.328041087056036 27.06783766501771 22.954839302114312 68-69 22.619919468485033 27.018569628704476 27.388815023126494 22.972695879683997 70-71 22.574906704926253 26.938303927290193 27.326130211178484 23.160659156605067 72-73 22.69229457515248 26.819947359419956 27.254171154824196 23.23358691060337 74-75 22.61309929424278 27.03064854126826 27.09402403114922 23.26222813333974 76-77 22.6939880492501 26.93629511813933 27.22230341877895 23.14741341383163 78-79 22.567711250374554 26.984212544988438 27.210083734423584 23.237992470213424 80-81 22.46893688476955 27.14929467767614 27.15370185285612 23.228066584698187 82-83 22.605699978039084 27.099060162451018 26.98959172118302 23.305648138326877 84-85 22.570628859535375 26.87935584359203 26.94041886795177 23.609596428920824 86-87 22.526874874676025 26.73520884590917 27.35489895231335 23.383017327101456 88-89 22.571151446521057 26.693876199365103 27.159361387686165 23.575610966427675 90-91 22.27250162484599 26.831864326356797 27.495534165626633 23.400099883170583 92-93 22.054460882792696 26.644993403807675 27.87218006218089 23.42836565121874 94-95 22.430780193688975 26.588524964046833 27.15905740953944 23.821637432724753 96-97 22.513588864750826 26.476459912724476 27.087286480262406 23.92266474226229 98-99 22.331381443422217 26.401309761217888 27.034422451540852 24.232886343819043 100-101 22.378401751424764 26.44987400955243 27.114055945910977 24.057668293111828 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3039.0 1 1748.5 2 506.0 3 519.0 4 440.0 5 462.5 6 604.5 7 967.5 8 1510.0 9 1768.5 10 1699.0 11 1635.5 12 1727.5 13 1857.0 14 2057.0 15 2276.0 16 2512.0 17 2802.0 18 3124.5 19 3506.0 20 4039.0 21 4746.5 22 5625.0 23 7162.0 24 9160.5 25 11913.5 26 16283.5 27 21661.0 28 27012.0 29 33134.0 30 40483.0 31 47905.5 32 55890.5 33 65267.5 34 75949.0 35 85144.0 36 95320.0 37 105261.5 38 111156.5 39 115135.0 40 118532.0 41 123770.5 42 129108.0 43 135702.0 44 142077.0 45 145568.0 46 147938.5 47 151587.5 48 159180.0 49 162579.5 50 160911.0 51 158891.5 52 153746.5 53 149423.0 54 144477.5 55 135071.0 56 124726.5 57 112726.0 58 99997.5 59 87977.5 60 74261.0 61 59368.5 62 46355.0 63 35927.5 64 27352.0 65 20245.0 66 14317.0 67 10334.0 68 7051.5 69 4301.0 70 2814.0 71 1826.0 72 1154.5 73 751.0 74 495.0 75 318.5 76 219.0 77 148.5 78 92.0 79 54.5 80 41.0 81 39.0 82 29.5 83 22.0 84 18.5 85 16.5 86 14.0 87 9.5 88 9.0 89 8.0 90 4.5 91 7.0 92 7.5 93 5.0 94 3.0 95 3.0 96 5.5 97 5.5 98 4.5 99 2.5 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08170263620875906 2 0.09179429130482117 3 0.08868725463731437 4 0.09132202173136014 5 0.08796642213045279 6 0.0735994845799013 7 0.0858287809032081 8 0.08212519319553999 9 0.07750192263428986 10-11 0.08372842411597349 12-13 0.08264717535568113 14-15 0.08799127842379284 16-17 0.08570449943650783 18-19 0.08835169467722363 20-21 0.09717567881294298 22-23 0.1066832110155138 24-25 0.12987413270178463 26-27 0.13493238839648572 28-29 0.13525552020990644 30-31 0.13663504449027944 32-33 0.13240947462247019 34-35 0.12794776996793042 36-37 0.13254618423584047 38-39 0.12445546075365276 40-41 0.12065244787262443 42-43 0.12025474717918354 44-45 0.11028737354982171 46-47 0.13014755192852523 48-49 0.14830507421343503 50-51 0.15811088193608652 52-53 0.16594061433820367 54-55 0.16191389481711485 56-57 0.13267046570254076 58-59 0.12476616442040345 60-61 0.12010560941914322 62-63 0.11712285421833668 64-65 0.1044461446149089 66-67 0.11760755193846775 68-69 0.11371754203074921 70-71 0.12331207126001024 72-73 0.15095226945415083 74-75 0.16377811681761895 76-77 0.16924650135243094 78-79 0.16597789877821376 80-81 0.1727015261266985 82-83 0.17150842404637587 84-85 0.14826778977342495 86-87 0.1500698710405789 88-89 0.17117286408628513 90-91 0.2594499898834886 92-93 0.5362745288116753 94-95 0.16877423177896989 96-97 0.18239548052931973 98-99 0.19991916733405815 100-101 0.2072393457227042 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 4023126.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.982575425234806 #Duplication Level Percentage of deduplicated Percentage of total 1 76.7237188593388 42.18467659088106 2 12.54904856327731 13.799580182906585 3 4.48207709307874 7.39308425495757 4 2.1085274058769863 4.637290685192243 5 1.1322648888064968 3.1127419825074156 6 0.6697342361054883 2.2094227890919216 7 0.4439324521849993 1.708598467517982 8 0.31704724827898295 1.3945659393489862 9 0.2371319245945558 1.1734311536776132 >10 1.1911828449445714 11.82313821084722 >50 0.08822516341317566 3.3619457061981377 >100 0.05380645162573961 5.395913506447101 >500 0.002385405064661684 0.8676202544405691 >1k 8.257170685895567E-4 0.6209718087274108 >5k 9.174634095439234E-5 0.31701846725810906 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7456 0.18532852314344617 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5113 0.12709022784769852 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 2.4856293340054474E-5 3 0.0 0.0 0.0 2.4856293340054474E-5 2.4856293340054474E-5 4 0.0 0.0 0.0 2.4856293340054474E-5 2.4856293340054474E-5 5 0.0 0.0 0.0 2.4856293340054474E-5 2.4856293340054474E-5 6 0.0 0.0 0.0 2.4856293340054474E-5 2.4856293340054474E-5 7 0.0 0.0 0.0 2.4856293340054474E-5 2.4856293340054474E-5 8 0.0 0.0 0.0 2.4856293340054474E-5 2.4856293340054474E-5 9 0.0 0.0 0.0 4.971258668010895E-5 2.4856293340054474E-5 10-11 0.0 0.0 0.0 4.971258668010895E-5 2.4856293340054474E-5 12-13 0.0 0.0 0.0 4.971258668010895E-5 2.4856293340054474E-5 14-15 0.0 0.0 0.0 8.699702669019066E-5 2.4856293340054474E-5 16-17 0.0 0.0 0.0 1.2428146670027238E-4 3.728444001008171E-5 18-19 0.0 0.0 0.0 1.2428146670027238E-4 4.971258668010895E-5 20-21 0.0 2.4856293340054474E-5 0.0 1.6156590671035409E-4 4.971258668010895E-5 22-23 0.0 2.4856293340054474E-5 0.0 2.982755200806537E-4 4.971258668010895E-5 24-25 0.0 2.4856293340054474E-5 0.0 4.225569867809261E-4 4.971258668010895E-5 26-27 0.0 2.4856293340054474E-5 0.0 7.33260653531607E-4 4.971258668010895E-5 28-29 0.0 2.4856293340054474E-5 0.0 0.001826937560494004 4.971258668010895E-5 30-31 0.0 3.728444001008171E-5 0.0 0.0053689593614517665 4.971258668010895E-5 32-33 0.0 6.214073335013619E-5 0.0 0.013372685816949307 4.971258668010895E-5 34-35 1.2428146670027237E-5 7.456888002016343E-5 0.0 0.026186105033747388 4.971258668010895E-5 36-37 2.4856293340054474E-5 7.456888002016343E-5 0.0 0.04702810699938306 4.971258668010895E-5 38-39 2.4856293340054474E-5 9.94251733602179E-5 2.4856293340054474E-5 0.0787571654479626 4.971258668010895E-5 40-41 2.4856293340054474E-5 9.94251733602179E-5 2.4856293340054474E-5 0.1157557580846337 4.971258668010895E-5 42-43 2.4856293340054474E-5 1.1185332003024514E-4 2.4856293340054474E-5 0.15448186310843856 4.971258668010895E-5 44-45 2.4856293340054474E-5 1.2428146670027238E-4 2.4856293340054474E-5 0.19969546069399766 4.971258668010895E-5 46-47 2.4856293340054474E-5 1.2428146670027238E-4 2.4856293340054474E-5 0.2542798808687573 4.971258668010895E-5 48-49 2.4856293340054474E-5 1.2428146670027238E-4 2.4856293340054474E-5 0.3152523684319109 4.971258668010895E-5 50-51 2.4856293340054474E-5 1.2428146670027238E-4 2.4856293340054474E-5 0.3831721899836098 4.971258668010895E-5 52-53 2.4856293340054474E-5 1.2428146670027238E-4 2.4856293340054474E-5 0.4520241225355607 4.971258668010895E-5 54-55 2.4856293340054474E-5 1.2428146670027238E-4 2.4856293340054474E-5 0.5284696527028983 4.971258668010895E-5 56-57 2.4856293340054474E-5 1.2428146670027238E-4 2.4856293340054474E-5 0.6153175416330485 4.971258668010895E-5 58-59 2.4856293340054474E-5 1.2428146670027238E-4 2.4856293340054474E-5 0.7166069369937704 4.971258668010895E-5 60-61 2.4856293340054474E-5 1.2428146670027238E-4 2.4856293340054474E-5 0.8281246970639249 4.971258668010895E-5 62-63 2.4856293340054474E-5 1.2428146670027238E-4 2.4856293340054474E-5 0.9459062430557731 4.971258668010895E-5 64-65 2.4856293340054474E-5 1.2428146670027238E-4 2.4856293340054474E-5 1.0648063222479236 4.971258668010895E-5 66-67 2.4856293340054474E-5 1.2428146670027238E-4 2.4856293340054474E-5 1.2020876303650445 4.971258668010895E-5 68-69 2.4856293340054474E-5 1.2428146670027238E-4 2.4856293340054474E-5 1.3615034677014837 4.971258668010895E-5 70-71 2.4856293340054474E-5 1.2428146670027238E-4 2.4856293340054474E-5 1.5430289779639017 4.971258668010895E-5 72-73 4.971258668010895E-5 1.2428146670027238E-4 2.4856293340054474E-5 1.730644280094633 4.971258668010895E-5 74-75 4.971258668010895E-5 1.2428146670027238E-4 2.4856293340054474E-5 1.9202605138392386 4.971258668010895E-5 76-77 4.971258668010895E-5 1.2428146670027238E-4 2.4856293340054474E-5 2.131178590976271 4.971258668010895E-5 78-79 4.971258668010895E-5 1.2428146670027238E-4 2.4856293340054474E-5 2.3696374411340835 4.971258668010895E-5 80-81 6.214073335013619E-5 1.3670961337029961E-4 2.4856293340054474E-5 2.6330768660986505 4.971258668010895E-5 82-83 8.699702669019066E-5 1.6156590671035409E-4 2.4856293340054474E-5 2.8994990462640247 4.971258668010895E-5 84-85 1.1185332003024514E-4 1.7399405338038132E-4 2.4856293340054474E-5 3.1605646952146165 4.971258668010895E-5 86-87 1.2428146670027238E-4 1.7399405338038132E-4 2.4856293340054474E-5 3.4460267960784723 4.971258668010895E-5 88-89 1.2428146670027238E-4 1.7399405338038132E-4 2.4856293340054474E-5 3.756022058468962 4.971258668010895E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 9680 0.0 38.825577 1 GGTATCA 5840 0.0 38.596584 1 ATCAACG 11885 0.0 31.143219 3 TCAACGC 12050 0.0 30.756159 4 TATCAAC 12240 0.0 30.550117 2 CAACGCA 12195 0.0 30.312637 5 AACGCAG 12600 0.0 29.371944 6 GTACATG 12020 0.0 28.108883 1 TACATGG 12165 0.0 27.032686 2 ACATGGG 12510 0.0 25.263004 3 ACGCAGA 14420 0.0 25.13835 7 CGCAGAG 14505 0.0 24.697563 8 GCAGAGT 15580 0.0 21.135714 9 CATGGGG 6555 0.0 19.690844 4 GAGTACT 7750 0.0 18.979385 12-13 CATGGGA 7580 0.0 18.90627 4 AGTACAT 8595 0.0 18.44033 2 AGAGTAC 13970 0.0 18.069025 10-11 GAGTACA 10120 0.0 17.818502 1 GTACTTT 8625 0.0 17.330297 14-15 >>END_MODULE