FastQCFastQC Report
Fri 27 May 2016
SRR939639_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939639_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4023126
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80050.19897462818713607No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59240.1472486817464827No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43120.1071803368823149No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA59700.041.792591
GTATCAA101500.036.59391
TCAACGC120150.030.3176864
ATCAACG120200.030.2659193
CAACGCA123300.029.5431425
TATCAAC128150.028.6108072
AACGCAG129150.028.3854456
GTACATG123950.024.8387051
ACGCAGA146750.024.692887
TACATGG124950.024.4843392
CGCAGAG148650.024.0933048
ACATGGG123500.023.8172663
GCAGAGT164750.020.8826949
CATGGGA75200.019.4009364
GAGTACT83800.018.59261312-13
CATGGGG61650.018.244994
AGAGTAC145300.018.21141410-11
GAGTACA102950.017.5591141
CAGAGTA157150.017.19706510-11
AGTACTT92000.017.13979312-13
Produced by FastQC (version 0.11.5)