##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939639_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4023126 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.52541605706607 31.0 31.0 34.0 30.0 34.0 2 31.744735561352044 31.0 31.0 34.0 30.0 34.0 3 31.794103391243524 31.0 31.0 34.0 30.0 34.0 4 35.5840694524606 37.0 35.0 37.0 33.0 37.0 5 35.312807751981914 37.0 35.0 37.0 33.0 37.0 6 35.32291357516518 37.0 35.0 37.0 32.0 37.0 7 35.2476613956411 37.0 35.0 37.0 32.0 37.0 8 35.20567563630868 37.0 35.0 37.0 32.0 37.0 9 36.82625102967195 39.0 37.0 39.0 32.0 39.0 10-11 36.700203026204 39.0 35.0 39.0 32.0 39.0 12-13 36.64235000345502 39.0 35.0 39.0 32.0 39.0 14-15 37.7312632017988 39.5 37.0 41.0 32.0 41.0 16-17 37.68508642284631 39.0 36.5 41.0 32.0 41.0 18-19 37.63420708175683 39.0 36.5 41.0 32.0 41.0 20-21 37.55676394425628 39.0 36.0 41.0 32.0 41.0 22-23 37.42126483237165 39.0 36.0 41.0 32.0 41.0 24-25 37.300029628701665 39.0 36.0 41.0 31.0 41.0 26-27 37.04964746319156 39.0 36.0 40.0 31.0 41.0 28-29 36.84987594223994 39.0 36.0 40.0 30.0 41.0 30-31 36.6325086512329 38.5 35.5 40.0 30.0 41.0 32-33 36.497778468782734 38.0 35.0 40.0 30.0 41.0 34-35 36.50861133854619 38.0 35.0 40.0 30.0 41.0 36-37 36.434404490438524 38.0 35.0 40.0 30.0 41.0 38-39 36.506727977199816 39.0 35.0 40.0 30.0 41.0 40-41 36.42496233028744 39.0 35.0 40.0 30.0 41.0 42-43 36.26554860573594 38.0 35.0 40.0 30.0 41.0 44-45 36.09576520844736 38.0 35.0 40.0 29.5 41.0 46-47 35.85362253133509 38.0 35.0 40.0 28.5 41.0 48-49 35.60929225184596 38.0 34.0 40.0 28.0 41.0 50-51 35.37325055193399 38.0 34.0 40.0 27.0 41.0 52-53 35.13580298007072 38.0 34.0 40.0 27.0 41.0 54-55 34.84206348496169 38.0 34.0 40.0 26.0 41.0 56-57 32.74946633538199 37.0 30.5 39.5 17.0 41.0 58-59 27.703919539184206 32.5 18.0 38.5 7.5 40.0 60-61 28.47916259644863 33.0 20.5 38.0 9.5 40.0 62-63 31.53191175220463 34.5 29.5 38.5 16.0 40.0 64-65 31.735935314976466 35.5 31.0 39.0 12.0 40.0 66-67 24.962725005381387 27.5 18.0 35.5 2.0 38.5 68-69 17.46985739447385 13.0 7.5 28.0 2.0 35.0 70-71 16.336750079415857 12.5 7.5 26.5 2.0 33.5 72-73 17.448559652369823 13.5 7.0 28.5 2.0 34.0 74-75 18.460542622826132 17.5 9.0 29.5 2.0 34.5 76-77 19.691177706092226 21.5 8.0 30.0 2.0 34.5 78-79 22.823673307771124 27.5 10.5 33.5 2.0 35.5 80-81 26.43508642284631 31.5 23.5 34.5 2.0 36.0 82-83 27.752571880671894 33.0 26.5 35.0 2.0 36.0 84-85 27.811929454856745 34.0 27.5 35.0 2.0 36.0 86-87 27.5665672663496 34.0 27.0 35.0 2.0 36.0 88-89 27.14703640900136 33.0 26.0 35.0 2.0 35.0 90-91 25.79161888044272 32.5 21.5 35.0 2.0 35.0 92-93 23.240652542326536 31.5 2.0 34.0 2.0 35.0 94-95 15.90802376062793 7.0 2.0 32.0 2.0 34.5 96-97 13.353026725983725 2.0 2.0 30.0 2.0 34.0 98-99 13.013228892159976 2.0 2.0 29.5 2.0 34.0 100-101 12.72611832192181 2.0 2.0 30.5 2.0 34.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.3844228898068387 1101 2 0.3654417965610719 1101 3 0.37899736764298453 1101 4 0.2878886708650157 1101 5 0.36394947944647527 1101 6 0.39588736068618147 1101 7 0.4770055674290603 1101 8 0.3505810295407841 1101 9 0.4403051713164814 1101 10-11 0.45866532280826533 1101 12-13 0.4176586268608773 1101 14-15 0.5331256493198069 1101 16-17 0.5503326494598895 1101 18-19 0.44822595363961426 1101 20-21 0.46673797242249293 1101 22-23 0.4961665372855393 1101 24-25 0.46804274806340374 1101 26-27 0.41389172261622065 1101 28-29 0.48373914015533614 1101 30-31 0.4774995636164121 1101 32-33 0.5009503902578345 1101 34-35 0.4721643223060994 1101 36-37 0.21989110127110933 1101 38-39 0.3642452952840003 1101 40-41 0.35017131279288094 1101 42-43 0.3754950919475988 1101 44-45 0.2735866726100866 1101 46-47 0.3565513107554992 1101 48-49 0.35100471119022103 1101 50-51 0.31965739123243964 1101 52-53 0.18733191215955713 1101 54-55 0.0987584466150011 1101 56-57 -2.3643365043197235 1101 58-59 -9.859056410228515 1101 60-61 -10.342738300942226 1101 62-63 -4.055353386925315 1101 64-65 -5.564264967286164 1101 66-67 -9.928482259109465 1101 68-69 -5.059043830266713 1101 70-71 -4.536786492910766 1101 72-73 -4.8513293530906125 1101 74-75 -6.914213345165667 1101 76-77 -8.320873970302952 1101 78-79 -11.264558333661085 1101 80-81 -8.343906613362375 1101 82-83 -4.720107442710255 1101 84-85 -3.9319793118600295 1101 86-87 -3.7760625867894113 1101 88-89 -3.9241365083122943 1101 90-91 -10.049364513564054 1101 92-93 -17.403415082139745 1101 94-95 -11.238414542942056 1101 96-97 -9.346531366417624 1101 98-99 -9.347225123859367 1101 100-101 -9.23967091020668 1102 1 0.2753095893142259 1102 2 0.3286191364625495 1102 3 0.3820077672051063 1102 4 0.23808252235196647 1102 5 0.26320372137313086 1102 6 0.314029579259433 1102 7 0.3739586781752777 1102 8 0.3368107321498002 1102 9 0.4588054449891672 1102 10-11 0.41895313525125033 1102 12-13 0.3910040474045715 1102 14-15 0.45308323005324525 1102 16-17 0.4173630727403079 1102 18-19 0.3955701882683371 1102 20-21 0.5119224734259546 1102 22-23 0.4953329758916354 1102 24-25 0.4597310804844952 1102 26-27 0.4186057346578238 1102 28-29 0.3504582607537685 1102 30-31 0.32966271815025294 1102 32-33 0.4111640830146328 1102 34-35 0.26106119331504374 1102 36-37 0.31035132750719896 1102 38-39 0.3501192233993109 1102 40-41 0.2880989263670557 1102 42-43 0.3097509302982715 1102 44-45 0.41388839674802114 1102 46-47 0.30624913049642544 1102 48-49 0.26772725269724873 1102 50-51 0.31906557404579416 1102 52-53 0.1776632841719632 1102 54-55 0.061651851103235344 1102 56-57 -3.199099777445067 1102 58-59 -10.34042842263501 1102 60-61 -10.563472427926353 1102 62-63 -4.4956026571314815 1102 64-65 -5.555842690329399 1102 66-67 -7.3110208013462845 1102 68-69 -4.715389615707327 1102 70-71 -4.44437520847362 1102 72-73 -4.8748743266355845 1102 74-75 -5.636726344618321 1102 76-77 -7.621793054411691 1102 78-79 -10.175839349898997 1102 80-81 -6.035373727027764 1102 82-83 -3.5972546330040025 1102 84-85 -3.2123284725971217 1102 86-87 -3.0208069308872076 1102 88-89 -3.15496915746391 1102 90-91 -9.285956832483958 1102 92-93 -13.784148524942152 1102 94-95 -10.70389415432684 1102 96-97 -9.347863240163292 1102 98-99 -9.30506014484094 1102 100-101 -9.215757618836491 1103 1 0.20096517612955012 1103 2 0.19136096550634463 1103 3 0.19136659461645067 1103 4 0.2100865050780527 1103 5 0.25495461574984546 1103 6 0.2135271074221734 1103 7 0.14179874393656888 1103 8 0.20920198867672468 1103 9 0.3727750882804699 1103 10-11 0.41743558925886504 1103 12-13 0.41258052384976196 1103 14-15 0.5216862657241137 1103 16-17 0.4625893008440016 1103 18-19 0.4264997032788145 1103 20-21 0.3055935280379316 1103 22-23 0.43479959161645354 1103 24-25 0.4942985957959891 1103 26-27 0.35062355272597046 1103 28-29 0.41727717407158593 1103 30-31 0.34614037899333283 1103 32-33 0.4220031183613244 1103 34-35 0.4834521603996649 1103 36-37 0.2737759509427633 1103 38-39 0.30310684684645395 1103 40-41 0.4237359309865525 1103 42-43 0.29049722833121194 1103 44-45 0.24969559344143732 1103 46-47 0.3402994124132306 1103 48-49 0.2166716566197735 1103 50-51 0.31233690883791354 1103 52-53 0.37676427638017884 1103 54-55 0.3398325325661915 1103 56-57 -2.813396779980579 1103 58-59 -10.30639058673722 1103 60-61 -10.649580065729833 1103 62-63 -4.272757555074122 1103 64-65 -5.364922784803369 1103 66-67 -6.65854925352463 1103 68-69 -4.319666125727819 1103 70-71 -4.176559166423441 1103 72-73 -4.503091188386364 1103 74-75 -5.256190760581642 1103 76-77 -7.107296915572007 1103 78-79 -9.56298608556871 1103 80-81 -5.217501533170889 1103 82-83 -2.9385155015624598 1103 84-85 -2.4766052141714567 1103 86-87 -2.4066980658483637 1103 88-89 -2.428770866331263 1103 90-91 -5.337486535053049 1103 92-93 -10.790954471244817 1103 94-95 -8.801812774729047 1103 96-97 -8.138862452880229 1103 98-99 -8.372148239500152 1103 100-101 -8.483506799714775 1104 1 0.19550486166777148 1104 2 0.20549477880581435 1104 3 0.15497225275952076 1104 4 0.14441650278666884 1104 5 0.1970280904492938 1104 6 0.12388835890238425 1104 7 0.19131431747336336 1104 8 0.20124109262332723 1104 9 0.24279539588559373 1104 10-11 0.23226914910683405 1104 12-13 0.2976511107063189 1104 14-15 0.3001598544679993 1104 16-17 0.28261468097753095 1104 18-19 0.2657987673098674 1104 20-21 0.3753441170634275 1104 22-23 0.370882060433523 1104 24-25 0.3505879188233436 1104 26-27 0.47873191942429827 1104 28-29 0.39910886408062396 1104 30-31 0.4808627105372949 1104 32-33 0.3732820077224872 1104 34-35 0.3426083365528356 1104 36-37 0.32800101727918474 1104 38-39 0.33535579604595256 1104 40-41 0.1807647936732053 1104 42-43 0.26274472573810925 1104 44-45 0.21502748547704442 1104 46-47 0.19870564147811365 1104 48-49 0.3671343936511491 1104 50-51 0.3380304021832785 1104 52-53 0.2664912694028274 1104 54-55 0.12757625443770593 1104 56-57 -3.0301235298716946 1104 58-59 -10.52254177956851 1104 60-61 -10.762618825094332 1104 62-63 -4.530199741368854 1104 64-65 -5.91286849825449 1104 66-67 -7.063275188312904 1104 68-69 -4.272278390401857 1104 70-71 -4.210363370325091 1104 72-73 -4.5628315798806085 1104 74-75 -5.345896151629855 1104 76-77 -7.029306091600988 1104 78-79 -9.55763010776889 1104 80-81 -5.441602077680578 1104 82-83 -3.0920464186231236 1104 84-85 -2.4885693846294004 1104 86-87 -2.3716681466764804 1104 88-89 -2.302642447403386 1104 90-91 -4.978156406158352 1104 92-93 -7.518463929761154 1104 94-95 -7.87193400815806 1104 96-97 -7.991646308946359 1104 98-99 -8.285342585555036 1104 100-101 -8.282163541717235 1105 1 0.2767998256965498 1105 2 0.3018976565036553 1105 3 0.27775402733985644 1105 4 0.24993209031664776 1105 5 0.1970280904492938 1105 6 0.21357660830286562 1105 7 0.33088266279710155 1105 8 0.20939456984154958 1105 9 0.34543328557384 1105 10-11 0.40169360953849065 1105 12-13 0.3483705351667439 1105 14-15 0.5085531398396981 1105 16-17 0.44892163541398844 1105 18-19 0.4134006857750947 1105 20-21 0.40124339763896444 1105 22-23 0.43407150887477286 1105 24-25 0.5031059044348538 1105 26-27 0.5922810800957521 1105 28-29 0.6967107825458498 1105 30-31 0.6510065954293793 1105 32-33 0.5941453170750037 1105 34-35 0.5211455068166231 1105 36-37 0.5047396263919026 1105 38-39 0.4523821749428336 1105 40-41 0.4245297816828 1105 42-43 0.32737302310021477 1105 44-45 0.36298911617247853 1105 46-47 0.47304616905604746 1105 48-49 0.4050240819005566 1105 50-51 0.4489416731665017 1105 52-53 0.44191093367141576 1105 54-55 0.40035802901804374 1105 56-57 1.6401402590971301 1105 58-59 -8.389208446235177 1105 60-61 -9.921851439003206 1105 62-63 -3.55897671978612 1105 64-65 -5.126417658925952 1105 66-67 -6.41255576385247 1105 68-69 -3.905971435965405 1105 70-71 -3.957006056176409 1105 72-73 -2.114030620647995 1105 74-75 -4.254529245155034 1105 76-77 -6.432183789442714 1105 78-79 -9.107270395538677 1105 80-81 -4.621338288711751 1105 82-83 -2.151398936608736 1105 84-85 -1.5847324541737642 1105 86-87 -1.5060806167004586 1105 88-89 -1.6275824953650186 1105 90-91 -4.570242737093604 1105 92-93 -7.169662970528538 1105 94-95 -8.126130650843912 1105 96-97 -8.248241033166984 1105 98-99 -8.439898940471103 1105 100-101 -8.388158745554165 1106 1 0.23404278419891256 1106 2 0.26050240239325007 1106 3 0.25415833618046335 1106 4 0.06227257752901494 1106 5 0.07583048082467769 1106 6 0.2162625697097198 1106 7 0.1746620539742878 1106 8 0.20269994084696208 1106 9 0.23492062454734253 1106 10-11 0.24734229334583802 1106 12-13 0.2712009319052058 1106 14-15 0.37336170614656083 1106 16-17 0.3240522271915154 1106 18-19 0.2654152027001899 1106 20-21 0.34968758031883596 1106 22-23 0.4457770640939529 1106 24-25 0.4854663359060538 1106 26-27 0.456241602572959 1106 28-29 0.49467094540256085 1106 30-31 0.42984357175497934 1106 32-33 0.36787742549690705 1106 34-35 0.4309333307273491 1106 36-37 0.36517871200175733 1106 38-39 0.3772944257084063 1106 40-41 0.3276827910274278 1106 42-43 0.1980609257237944 1106 44-45 0.1022268308209604 1106 46-47 0.11350503379287602 1106 48-49 0.19602714784871011 1106 50-51 0.29616112790110094 1106 52-53 0.3324705288414904 1106 54-55 0.1748834125367864 1106 56-57 1.5456085088278755 1106 58-59 -8.488145639250465 1106 60-61 -10.024608131889142 1106 62-63 -3.867273311281096 1106 64-65 -5.610875723690668 1106 66-67 -7.235573676989443 1106 68-69 -4.385389676430417 1106 70-71 -3.9427282970524153 1106 72-73 -4.327041634942908 1106 74-75 -4.959232750904375 1106 76-77 -6.456888065807393 1106 78-79 -9.10676389943487 1106 80-81 -4.671776713794181 1106 82-83 -2.2936836092531756 1106 84-85 -1.8609325499502454 1106 86-87 -1.675463885907586 1106 88-89 -1.4694513109475942 1106 90-91 -4.1803169730376695 1106 92-93 -6.529001635786081 1106 94-95 -7.538762871005044 1106 96-97 -7.547371889155491 1106 98-99 -7.048515612550425 1106 100-101 -7.49041949242721 1107 1 0.027039433389425938 1107 2 -0.03151597561906527 1107 3 -0.016585194380450474 1107 4 0.042540134285104614 1107 5 0.061197132574086766 1107 6 0.005746445397129207 1107 7 0.1269345053450337 1107 8 0.16038097918553262 1107 9 0.14550046851246634 1107 10-11 0.0906443930244265 1107 12-13 0.039670144154023035 1107 14-15 0.1288470265359365 1107 16-17 0.039451819339447525 1107 18-19 0.06493950726149222 1107 20-21 0.10960735848843939 1107 22-23 0.11966724055310607 1107 24-25 0.1331630815257583 1107 26-27 0.07360353586413027 1107 28-29 0.194262843595844 1107 30-31 0.09900164577076254 1107 32-33 0.004120866609106599 1107 34-35 0.040673605379090816 1107 36-37 0.1465432506050135 1107 38-39 0.11871564745310792 1107 40-41 0.11969739251890132 1107 42-43 0.06364273195696768 1107 44-45 0.07231328330163933 1107 46-47 0.18744322079607656 1107 48-49 0.032623669295361424 1107 50-51 0.10273659280756675 1107 52-53 0.02756711853961491 1107 54-55 -0.03962117462644699 1107 56-57 1.2387066794263504 1107 58-59 -9.007122321487973 1107 60-61 -10.687686738282292 1107 62-63 -4.247581508274411 1107 64-65 -5.7559517770339035 1107 66-67 -7.179999644907166 1107 68-69 -3.953382401389387 1107 70-71 -3.907825539114061 1107 72-73 -2.5255633767693446 1107 74-75 -4.922768553875242 1107 76-77 -6.958275053999735 1107 78-79 -7.861670620283647 1107 80-81 -2.731523405485781 1107 82-83 -1.5453523289462119 1107 84-85 -1.238949830213226 1107 86-87 -1.2067096620669844 1107 88-89 -1.0898764290263045 1107 90-91 -0.5850401945658881 1107 92-93 -3.522476721438487 1107 94-95 -6.193853343957631 1107 96-97 -6.69123232916768 1107 98-99 -6.479973677910671 1107 100-101 -7.128431137146505 1108 1 -0.055686690276200324 1108 2 -0.12399996602979968 1108 3 -0.16379954703963762 1108 4 -0.07978836931472699 1108 5 -0.031072627869804137 1108 6 -0.15547354912823153 1108 7 -0.10325542889594175 1108 8 -0.08831788476263824 1108 9 -0.09844862203673443 1108 10-11 -0.10695379841668995 1108 12-13 -0.1448960453922652 1108 14-15 -0.21881267895951595 1108 16-17 -0.12352756355825534 1108 18-19 -0.06701192352442575 1108 20-21 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88-89 1.4505830942724316 2316 90-91 2.5116208562130637 2316 92-93 4.812262793208298 2316 94-95 11.945399562543194 2316 96-97 13.762901948839641 2316 98-99 13.410775672474989 2316 100-101 12.290499937120416 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7.0 3 26.0 4 72.0 5 117.0 6 270.0 7 706.0 8 1692.0 9 2991.0 10 4786.0 11 7498.0 12 11411.0 13 16417.0 14 20609.0 15 28719.0 16 39938.0 17 53246.0 18 62523.0 19 67526.0 20 71171.0 21 71500.0 22 71104.0 23 60303.0 24 60637.0 25 71601.0 26 101358.0 27 152426.0 28 215726.0 29 292901.0 30 359855.0 31 390807.0 32 387825.0 33 355705.0 34 317495.0 35 285899.0 36 232537.0 37 147679.0 38 54552.0 39 3491.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.24644307502895 19.48160115201954 13.711757431465827 24.56019834148568 2 15.759293374654067 20.738339267368143 39.24613935581315 24.25622800216464 3 19.05450425627691 24.668310665585913 29.806490837825493 26.470694240311676 4 13.040058899472697 16.82179479340195 36.93844040678815 33.1997059003372 5 13.611886908307202 37.65807118495489 34.81148937049295 13.91855253624496 6 32.56693924077943 36.71088104125996 17.200579847611035 13.521599870349574 7 28.65480723198826 32.169238547338566 21.261327634282395 17.91462658639078 8 26.07258609559845 36.04261634871194 19.752665696757465 18.13213185893215 9 26.306235499459873 16.149581196313516 19.901837526341456 37.642345777885154 10-11 24.878353300393773 26.508578155394584 27.993816251342864 20.61925229286878 12-13 25.628093676409836 23.586273459991062 28.243982415663837 22.541650447935265 14-15 22.537368877560976 25.06288371369296 25.857308312332737 26.54243909641333 16-17 22.698742221844405 28.342400909143784 26.42010217925066 22.538754689761145 18-19 22.574413036253713 27.311297528111893 28.078314362562978 22.035975073071416 20-21 23.12971306392094 26.675525449613062 28.035537539714145 22.159223946751855 22-23 22.831725436462442 26.594906306994897 27.877060360603885 22.696307895938777 24-25 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27.296135171157985 22.73455150241531 50-51 22.76947435172169 27.051886525433293 27.33879649596844 22.839842626876575 52-53 23.00467433810892 26.973589038124235 27.251693435365482 22.770043188401363 54-55 22.7808605722854 26.81876998986608 27.418123946248933 22.982245491599592 56-57 21.92679627924788 27.14124095226978 28.353887390081812 22.57807537840053 58-59 21.527229252744924 26.435183559665955 29.618949851333053 22.41863733625607 60-61 22.77020742009582 26.775102591829764 27.948744378454997 22.505945609619424 62-63 22.890992598212208 26.929884982327636 27.424733659180696 22.75438876027947 64-65 22.40151985729189 26.83178241791496 27.799424727843213 22.967272996949934 66-67 22.115661340593874 26.65657507139413 28.85863804016066 22.369125547851333 68-69 22.24210858028299 25.95090369225799 29.637286652571714 22.169701074887303 70-71 22.122829685942264 25.965109754219913 29.584750882243565 22.32730967759426 72-73 21.38410921740161 26.12054220550224 29.959747252685514 22.535601324410635 74-75 22.05382216317761 26.27027389099368 29.022631249372033 22.653272696456682 76-77 20.386080533243494 26.9300117917986 29.503175917697483 23.18073175726043 78-79 21.153585307611163 26.88107078590794 28.755990088866184 23.209353817614712 80-81 22.068555933842283 27.101488184318633 27.54981992244784 23.280135959391245 82-83 22.592119171264574 27.044860111592566 27.072574937222193 23.29044577992067 84-85 22.60315938951843 26.845054794551192 27.01314577073161 23.538640045198765 86-87 22.480571020358855 26.75981838943664 27.39481158127759 23.364799008926916 88-89 22.544755043471216 26.750195494941494 27.187194422639934 23.517855038947364 90-91 21.939574601486374 26.944335558103482 27.78599443936216 23.330095401047984 92-93 21.022982791606086 26.970789603588525 28.34134182248157 23.664885782323825 94-95 20.011577138361265 26.682893604626894 29.498271992217973 23.807257264793865 96-97 20.048537281820483 26.70326413729874 29.2134656811757 24.034732899705077 98-99 20.449326529719283 26.61656132009985 28.557739993652348 24.376372156528518 100-101 20.89844397038524 26.607842891203415 28.2932488392521 24.20046429915924 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 259.0 1 209.5 2 184.0 3 222.0 4 234.0 5 281.5 6 381.5 7 544.5 8 769.0 9 955.5 10 1100.5 11 1287.5 12 1519.5 13 1759.5 14 2029.0 15 2318.5 16 2572.5 17 2912.0 18 3340.5 19 3812.5 20 4419.5 21 5212.5 22 6419.0 23 7919.5 24 10134.5 25 13226.5 26 17216.5 27 22316.5 28 28214.0 29 34756.5 30 41880.0 31 50106.0 32 59763.0 33 70065.0 34 80417.5 35 90629.5 36 100664.5 37 109660.5 38 116859.0 39 122988.0 40 128913.5 41 134725.0 42 140337.5 43 146139.5 44 151360.5 45 155443.0 46 159061.5 47 162834.5 48 166041.0 49 167150.5 50 165467.5 51 160912.5 52 154878.5 53 148145.0 54 139193.0 55 127574.5 56 114927.0 57 101675.0 58 87255.0 59 72488.5 60 58391.5 61 45606.0 62 34542.5 63 25494.0 64 18488.5 65 13056.5 66 8887.5 67 5916.0 68 3771.0 69 2183.5 70 1212.0 71 708.5 72 403.0 73 236.0 74 139.0 75 59.0 76 25.5 77 20.5 78 11.5 79 4.5 80 6.0 81 5.0 82 0.5 83 1.0 84 1.0 85 0.0 86 0.0 87 1.0 88 1.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.024160317126532948 2 0.007133756188595634 3 2.237066400604903E-4 4 0.0 5 0.1340997025695939 6 0.0 7 0.0 8 0.0017150842404637587 9 0.0 10-11 0.0 12-13 0.0 14-15 6.338354801713891E-4 16-17 0.0 18-19 1.7399405338038132E-4 20-21 0.0 22-23 3.3555996009073546E-4 24-25 0.0 26-27 3.3555996009073546E-4 28-29 0.003368027747577381 30-31 0.01188130821654604 32-33 0.002883330027446319 34-35 0.004685411294600268 36-37 0.1034891773213168 38-39 0.10235821597434432 40-41 0.11497278484442197 42-43 0.11803010892524868 44-45 0.11345655095067865 46-47 0.10669563916218383 48-49 0.10639736364210317 50-51 0.10612394441536259 52-53 0.0848221010229359 54-55 0.07367405345992147 56-57 0.5601862830048077 58-59 1.5976879670186814 60-61 0.5275375417026462 62-63 0.004449276507869751 64-65 0.12065244787262443 66-67 1.9288110787482171 68-69 2.9022953792647805 70-71 2.813931256440887 72-73 2.662606142586635 74-75 2.034487609883459 76-77 1.4331641614008608 78-79 0.9523564511775172 80-81 0.055168543068250905 82-83 2.1127849339046305E-4 84-85 1.7399405338038132E-4 86-87 2.982755200806537E-4 88-89 9.942517336021791E-5 90-91 0.25544812665573985 92-93 0.4008822989883986 94-95 2.0316912768827025 96-97 2.453813278530178 98-99 1.4386946866690231 100-101 0.9600991865529441 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 4023126.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.84325183367197 #Duplication Level Percentage of deduplicated Percentage of total 1 72.17610309237918 35.25315579714147 2 14.619214547568053 14.280999555146945 3 5.249736929442491 7.6924266871586235 4 2.5790499457472174 5.038767439669975 5 1.438048946429073 3.511949341979094 6 0.8779312774428418 2.572861108607883 7 0.6017189847182284 2.057293833258569 8 0.42115378445785223 1.6456416283983106 9 0.3039521674901059 1.3361411035898718 >10 1.5364006767023686 13.491366615874686 >50 0.11805040327600863 4.00814904760535 >100 0.07318980486866244 6.593345126525335 >500 0.0041762060400234065 1.3091005017168398 >1k 0.0011713747628667491 0.8623107528703473 >5k 1.0185867503189088E-4 0.34649146045668666 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8005 0.19897462818713607 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5924 0.1472486817464827 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4312 0.1071803368823149 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 2.4856293340054474E-5 0.0 10-11 0.0 0.0 0.0 2.4856293340054474E-5 0.0 12-13 0.0 0.0 0.0 2.4856293340054474E-5 0.0 14-15 0.0 0.0 0.0 6.214073335013619E-5 0.0 16-17 0.0 0.0 0.0 9.94251733602179E-5 0.0 18-19 0.0 0.0 0.0 1.2428146670027238E-4 0.0 20-21 0.0 2.4856293340054474E-5 0.0 1.6156590671035409E-4 0.0 22-23 1.2428146670027237E-5 2.4856293340054474E-5 0.0 2.8584737341062646E-4 0.0 24-25 2.4856293340054474E-5 3.728444001008171E-5 0.0 3.728444001008171E-4 0.0 26-27 2.4856293340054474E-5 7.456888002016343E-5 0.0 6.711199201814708E-4 0.0 28-29 2.4856293340054474E-5 7.456888002016343E-5 0.0 0.001777224973813895 0.0 30-31 2.4856293340054474E-5 7.456888002016343E-5 0.0 0.00558023785484223 0.0 32-33 2.4856293340054474E-5 7.456888002016343E-5 0.0 0.013745530217050123 0.0 34-35 3.728444001008171E-5 7.456888002016343E-5 0.0 0.02694422198061905 0.0 36-37 4.971258668010895E-5 7.456888002016343E-5 0.0 0.048531912746456365 0.0 38-39 4.971258668010895E-5 7.456888002016343E-5 0.0 0.08128007922197814 0.0 40-41 4.971258668010895E-5 7.456888002016343E-5 0.0 0.11960848355234213 0.0 42-43 4.971258668010895E-5 9.94251733602179E-5 0.0 0.1598881069099004 0.0 44-45 4.971258668010895E-5 9.94251733602179E-5 0.0 0.2070156390826437 0.0 46-47 4.971258668010895E-5 9.94251733602179E-5 0.0 0.2610905052439322 0.0 48-49 4.971258668010895E-5 9.94251733602179E-5 0.0 0.3126051731911951 0.0 50-51 4.971258668010895E-5 9.94251733602179E-5 0.0 0.3646169670052591 0.0 52-53 6.214073335013619E-5 9.94251733602179E-5 0.0 0.417511159232895 0.0 54-55 7.456888002016343E-5 9.94251733602179E-5 0.0 0.47419593619488926 0.0 56-57 7.456888002016343E-5 9.94251733602179E-5 0.0 0.5200060848206096 0.0 58-59 7.456888002016343E-5 9.94251733602179E-5 0.0 0.553922497083114 0.0 60-61 7.456888002016343E-5 9.94251733602179E-5 0.0 0.5815626952772546 0.0 62-63 1.988503467204358E-4 9.94251733602179E-5 0.0 0.6077612284576719 0.0 64-65 1.988503467204358E-4 9.94251733602179E-5 0.0 0.6331892165445476 0.0 66-67 1.988503467204358E-4 1.4913776004032685E-4 0.0 0.6544040629102842 0.0 68-69 2.1127849339046305E-4 1.7399405338038132E-4 0.0 0.6863195435589141 0.0 70-71 2.60991080070572E-4 1.7399405338038132E-4 0.0 0.7366659657191945 0.0 72-73 2.7341922674059923E-4 1.7399405338038132E-4 0.0 0.8078295335517705 0.0 74-75 2.7341922674059923E-4 1.7399405338038132E-4 0.0 0.9001830914567428 0.0 76-77 2.7341922674059923E-4 1.7399405338038132E-4 0.0 1.0488361537769386 0.0 78-79 2.982755200806537E-4 1.7399405338038132E-4 0.0 1.2724060842240585 0.0 80-81 2.982755200806537E-4 1.7399405338038132E-4 0.0 1.5465088590315093 0.0 82-83 2.982755200806537E-4 1.7399405338038132E-4 0.0 1.8211957567324513 2.4856293340054474E-5 84-85 2.982755200806537E-4 1.8642220005040856E-4 0.0 2.032896807109695 2.4856293340054474E-5 86-87 2.982755200806537E-4 1.988503467204358E-4 0.0 2.1983402955810982 2.4856293340054474E-5 88-89 2.982755200806537E-4 1.988503467204358E-4 0.0 2.358116549170968 2.4856293340054474E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5970 0.0 41.79259 1 GTATCAA 10150 0.0 36.5939 1 TCAACGC 12015 0.0 30.317686 4 ATCAACG 12020 0.0 30.265919 3 CAACGCA 12330 0.0 29.543142 5 TATCAAC 12815 0.0 28.610807 2 AACGCAG 12915 0.0 28.385445 6 GTACATG 12395 0.0 24.838705 1 ACGCAGA 14675 0.0 24.69288 7 TACATGG 12495 0.0 24.484339 2 CGCAGAG 14865 0.0 24.093304 8 ACATGGG 12350 0.0 23.817266 3 GCAGAGT 16475 0.0 20.882694 9 CATGGGA 7520 0.0 19.400936 4 GAGTACT 8380 0.0 18.592613 12-13 CATGGGG 6165 0.0 18.24499 4 AGAGTAC 14530 0.0 18.211414 10-11 GAGTACA 10295 0.0 17.559114 1 CAGAGTA 15715 0.0 17.197065 10-11 AGTACTT 9200 0.0 17.139793 12-13 >>END_MODULE