FastQCFastQC Report
Fri 27 May 2016
SRR939638_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939638_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4031030
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA45800.051.1729741
GTATCAA75800.048.5882841
TATCAAC110050.033.2702752
ATCAACG116350.031.3067593
GTACATG98850.031.3052581
TCAACGC118700.030.4841564
CAACGCA120300.030.0547945
AACGCAG125150.029.0412886
ACGCAGA133550.026.4014247
TACATGG118950.026.0148642
ACATGGG121400.024.2837663
CGCAGAG145400.023.9581558
GCAGAGT153400.021.1391439
GAGTACA91700.019.6944941
CATGGGA72350.019.5874924
GAGTACT76300.018.71404612-13
AGTACAT85900.018.699522
CATGGGG63200.018.3123934
AGAGTAC138400.018.02434710-11
GTACTTT80550.017.49496514-15
Produced by FastQC (version 0.11.5)