FastQCFastQC Report
Fri 27 May 2016
SRR939638_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939638_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4031030
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79920.19826198266944178No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59170.14678630523712302No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44110.10942612682118466No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA59700.039.8400421
GTATCAA99750.036.191961
ATCAACG122350.029.1512623
TCAACGC121900.029.142774
CAACGCA125550.028.3331055
TATCAAC128900.027.5998382
AACGCAG130050.027.3703166
GTACATG123950.026.7272431
TACATGG124550.025.646792
ACATGGG125300.024.0220013
ACGCAGA150000.023.3528987
CGCAGAG151500.022.8727238
GCAGAGT168000.019.8966589
TGCACCG10350.019.6005235
CATGGGA72000.019.5264534
CATGGGG64700.018.4482774
GAGTACT81500.018.30900412-13
AGAGTAC149400.018.01891710-11
AGTACTT90800.017.6539412-13
CAGAGTA159050.016.61441810-11
Produced by FastQC (version 0.11.5)