FastQCFastQC Report
Fri 27 May 2016
SRR939637_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939637_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1843258
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109670.5949791076452673No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70270.38122715322543016No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56130.30451515740064605No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA45270.2455977405224879No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA35970.19514359899699335No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG110450.030.4602621
TACATGG112800.029.5732692
ACATGGG113000.028.0511823
CATGGGA75500.025.2015324
GTATCAA166150.024.4756151
CATGGGG39100.023.663964
GAGTACT79650.022.72865312-13
ATGGGAG28900.021.8365155
GGTATCA122800.021.4074291
ATGGGAT31050.021.241385
AGTACTT84400.021.1402612-13
TATCAAC192500.020.9774152
ATCAACG195500.020.7283253
TCAACGC195550.020.6976364
CAACGCA195700.020.6332825
AACGCAG199650.020.3908676
AGTACAT77200.020.2838232
GTACTTT90150.020.21353314-15
GTATAGG4550.018.772161
GTATATG8600.018.7600331
Produced by FastQC (version 0.11.5)