##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939637_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1843258 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.342379634321404 31.0 30.0 33.0 26.0 34.0 2 30.467899230601468 31.0 30.0 34.0 26.0 34.0 3 30.503233947716488 31.0 30.0 34.0 26.0 34.0 4 34.115681038682595 35.0 35.0 37.0 30.0 37.0 5 34.11899961915261 35.0 35.0 37.0 30.0 37.0 6 34.25354562410688 36.0 35.0 37.0 30.0 37.0 7 34.05658730356792 36.0 35.0 37.0 29.0 37.0 8 34.07707711020378 36.0 35.0 37.0 29.0 37.0 9 35.345543597260935 38.0 35.0 39.0 29.0 39.0 10-11 35.23552617159399 37.0 35.0 39.0 28.5 39.0 12-13 35.08298404238582 37.0 34.5 39.0 27.5 39.0 14-15 35.73896654727662 38.0 34.0 40.0 27.0 41.0 16-17 35.48635568108209 38.0 34.0 40.0 27.0 41.0 18-19 35.51084953923976 38.0 34.0 40.0 27.0 41.0 20-21 35.647322295630886 38.0 34.0 40.0 27.0 41.0 22-23 35.49869660134392 38.0 34.0 40.0 27.0 41.0 24-25 35.35821165566621 38.0 34.0 40.0 27.0 41.0 26-27 35.449482112650536 38.0 34.0 40.0 27.0 41.0 28-29 35.31698248427513 38.0 34.0 40.0 26.0 41.0 30-31 35.06150875243726 38.0 34.0 40.0 25.0 41.0 32-33 34.797421467857454 38.0 33.5 40.0 25.0 41.0 34-35 34.540598494622024 38.0 33.0 40.0 24.0 41.0 36-37 34.219921465144864 38.0 33.0 40.0 24.0 41.0 38-39 34.02178018486831 38.0 33.0 40.0 23.5 41.0 40-41 33.709657573709165 38.0 32.5 40.0 21.5 41.0 42-43 33.35587693095595 37.0 32.0 40.0 19.0 41.0 44-45 33.05155843620372 37.0 31.5 40.0 17.0 41.0 46-47 32.52172756065619 37.0 31.0 40.0 14.0 41.0 48-49 32.109950424737065 37.0 30.5 39.5 9.0 41.0 50-51 31.481418770459697 35.5 30.0 38.5 9.0 40.0 52-53 31.515556964895854 36.0 30.0 39.0 8.0 40.0 54-55 31.555735822114972 36.0 30.0 39.0 7.0 40.0 56-57 31.427825079288954 36.0 30.0 39.0 6.0 40.0 58-59 31.034914808453294 35.5 29.0 39.0 2.0 40.0 60-61 30.611184109875012 35.0 28.0 39.0 2.0 40.0 62-63 30.173936855285586 35.0 28.0 38.0 2.0 40.0 64-65 29.5414865960164 34.0 27.5 38.0 2.0 40.0 66-67 29.00742299775723 34.0 26.0 37.5 2.0 39.5 68-69 28.499746915515896 34.0 26.0 37.0 2.0 39.0 70-71 27.932897619324045 33.0 25.5 36.0 2.0 39.0 72-73 27.184789107113602 32.5 24.0 36.0 2.0 38.5 74-75 26.7234847210754 32.0 23.0 35.0 2.0 38.0 76-77 26.327365729594014 32.0 22.0 35.0 2.0 37.0 78-79 25.746682233306462 31.5 20.5 35.0 2.0 36.5 80-81 25.076574467600302 31.0 18.5 34.5 2.0 36.0 82-83 24.399330695974193 31.0 15.5 34.0 2.0 35.5 84-85 23.733081044541784 30.0 8.5 34.0 2.0 35.0 86-87 23.03046941882254 30.0 4.5 34.0 2.0 35.0 88-89 22.18564601374306 29.0 2.0 34.0 2.0 35.0 90-91 20.90233651501852 28.0 2.0 33.5 2.0 35.0 92-93 19.240832265477756 25.0 2.0 32.0 2.0 34.0 94-95 18.966637605804507 25.0 2.0 32.0 2.0 34.0 96-97 18.313757216841047 25.0 2.0 32.0 2.0 34.0 98-99 17.298437874676253 22.0 2.0 32.0 2.0 34.0 100-101 15.659476047303198 9.5 2.0 30.5 2.0 34.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.8378924202450158 1101 2 1.0261612044919204 1101 3 0.9153716251005939 1101 4 1.0635420548731105 1101 5 0.8895788839369914 1101 6 0.924226059011211 1101 7 0.7569267730537277 1101 8 0.8510048930240401 1101 9 1.2928637310747888 1101 10-11 1.0208503560788316 1101 12-13 0.8498412783524643 1101 14-15 1.0544120252155054 1101 16-17 1.000166860798437 1101 18-19 1.002630376365424 1101 20-21 1.204943090993119 1101 22-23 1.3250756103178034 1101 24-25 1.3010485961758533 1101 26-27 1.0822466997812583 1101 28-29 1.2534460637582399 1101 30-31 1.4266915888905984 1101 32-33 1.1452866800676276 1101 34-35 1.1042222387777372 1101 36-37 1.205548525979232 1101 38-39 1.330919653742086 1101 40-41 1.3958024419514174 1101 42-43 1.2573630406878138 1101 44-45 1.091625372014029 1101 46-47 1.0902188838653828 1101 48-49 0.9069360442459882 1101 50-51 0.8889123928090044 1101 52-53 1.0525923785727116 1101 54-55 1.015858638295363 1101 56-57 0.9826468108584443 1101 58-59 0.8826751784374842 1101 60-61 0.8555178596767696 1101 62-63 0.7898763139886746 1101 64-65 0.798228338168748 1101 66-67 0.6773524347849609 1101 68-69 0.582204874867017 1101 70-71 0.4613619525353734 1101 72-73 0.7386654142424298 1101 74-75 0.7606384028129582 1101 76-77 0.0059558473644010235 1101 78-79 0.13161902325328256 1101 80-81 0.005437317876651804 1101 82-83 -0.009185677771444745 1101 84-85 -0.5737347407578781 1101 86-87 -0.8633529055916895 1101 88-89 -1.3169578662446675 1101 90-91 -4.45372638471984 1101 92-93 -9.580828024778908 1101 94-95 -8.042054571962169 1101 96-97 -5.020501253383388 1101 98-99 -4.014565476922607 1101 100-101 -3.3295791756558692 1102 1 0.8774919671635999 1102 2 0.8255524726575558 1102 3 0.7632526218797082 1102 4 0.8425785633722427 1102 5 0.7151232751281 1102 6 0.744229064143056 1102 7 0.8517575610147077 1102 8 0.6705432585405333 1102 9 0.754186143193948 1102 10-11 0.7522701652914847 1102 12-13 0.6441455903313837 1102 14-15 0.7096575213704739 1102 16-17 0.7668158691049385 1102 18-19 0.7370198722738195 1102 20-21 0.7377547000485762 1102 22-23 0.8981272831745315 1102 24-25 0.895021187832377 1102 26-27 0.7875126154218108 1102 28-29 1.015283201023415 1102 30-31 1.1092385151801025 1102 32-33 0.945852646564262 1102 34-35 0.930618083990332 1102 36-37 0.7793576461690961 1102 38-39 0.7554557846733729 1102 40-41 0.8399734493640736 1102 42-43 0.7820808279861282 1102 44-45 0.6785944653888762 1102 46-47 0.6009383432498652 1102 48-49 0.7804367531488836 1102 50-51 0.6380158618099117 1102 52-53 0.589981612495265 1102 54-55 0.6731905820762805 1102 56-57 0.661071890610021 1102 58-59 0.880408615537764 1102 60-61 0.6550522568781467 1102 62-63 0.474147145716703 1102 64-65 0.4384354611017578 1102 66-67 0.5154087694487721 1102 68-69 0.34169049646698113 1102 70-71 0.3305121551891368 1102 72-73 0.24926312726004696 1102 74-75 0.16254646889503732 1102 76-77 0.08806221362062061 1102 78-79 -0.14495327713661155 1102 80-81 -0.36991328083807673 1102 82-83 -0.31420636694834414 1102 84-85 -0.6404037832766427 1102 86-87 -1.0054120373397524 1102 88-89 -1.5967292835881324 1102 90-91 -5.648374167857199 1102 92-93 -10.443964496291798 1102 94-95 -8.993766533927987 1102 96-97 -5.754963373311265 1102 98-99 -4.595316066390776 1102 100-101 -4.097651190943514 1103 1 0.8410217089677481 1103 2 0.9428475894240087 1103 3 0.9001451805404699 1103 4 0.8612089897081887 1103 5 0.7998173960755537 1103 6 0.7482819484627257 1103 7 0.8339522332780405 1103 8 0.8050475318011863 1103 9 0.756838460145822 1103 10-11 0.7284363671526037 1103 12-13 0.7727951139494493 1103 14-15 0.8282454926330374 1103 16-17 0.9060427067120287 1103 18-19 0.8850779652159275 1103 20-21 0.7906909259275707 1103 22-23 0.8812135119788707 1103 24-25 0.8079608341201023 1103 26-27 0.7629764333480509 1103 28-29 0.734244416888707 1103 30-31 0.9479192875324003 1103 32-33 1.0527863251388467 1103 34-35 0.8660413670726612 1103 36-37 1.099874397920935 1103 38-39 0.9921933748153862 1103 40-41 0.994280389043368 1103 42-43 1.0517143491919043 1103 44-45 0.9312123532264636 1103 46-47 0.8553565962310969 1103 48-49 0.7953257323818974 1103 50-51 0.5303455952130491 1103 52-53 0.7309914131664428 1103 54-55 0.6092965967450397 1103 56-57 0.4190613203468736 1103 58-59 0.28473258225178455 1103 60-61 0.1398660921314594 1103 62-63 0.29947122194381137 1103 64-65 0.14106587543627214 1103 66-67 0.03317149209413017 1103 68-69 0.11550606742770952 1103 70-71 0.3738631356313 1103 72-73 0.2688166139017021 1103 74-75 -0.1752170938507085 1103 76-77 -0.6081655856013874 1103 78-79 -0.3686412578503102 1103 80-81 -0.46699063158148846 1103 82-83 -0.8295473501958455 1103 84-85 -0.8093394210853582 1103 86-87 -1.2058650914797227 1103 88-89 -1.449775172571865 1103 90-91 -2.886763155341203 1103 92-93 -9.21840729220591 1103 94-95 -5.725946129389644 1103 96-97 -3.2027187300763984 1103 98-99 -2.3798759212934364 1103 100-101 -2.034532798295592 1104 1 0.8057642631389967 1104 2 0.8646882952990502 1104 3 0.8110284829265133 1104 4 0.8335619909595735 1104 5 0.7795259178068719 1104 6 0.794757209481709 1104 7 0.7738271677506745 1104 8 0.8655071884913283 1104 9 0.8364755972051796 1104 10-11 0.6611408380062116 1104 12-13 0.6385507924002951 1104 14-15 0.8235273915645678 1104 16-17 0.8967958711582327 1104 18-19 0.8184992865349727 1104 20-21 0.8284218911251244 1104 22-23 0.8355675459964189 1104 24-25 0.7618057985231061 1104 26-27 0.6883225105735775 1104 28-29 0.789134475874441 1104 30-31 0.740035786899135 1104 32-33 0.7822241832539163 1104 34-35 0.7678430399239957 1104 36-37 0.5716976951300907 1104 38-39 0.46953951577256703 1104 40-41 0.5061067892525344 1104 42-43 0.4682896192743655 1104 44-45 0.3942200483008165 1104 46-47 0.3043067609539065 1104 48-49 0.28267643642073637 1104 50-51 0.4741052871906746 1104 52-53 0.47037480422828537 1104 54-55 0.4706051642377673 1104 56-57 0.3430831374668166 1104 58-59 0.3095941944587963 1104 60-61 0.31276679378035865 1104 62-63 0.26451415069522 1104 64-65 0.19598009498605862 1104 66-67 0.3436747030983902 1104 68-69 0.26131219836020136 1104 70-71 0.32427938950058177 1104 72-73 0.3239710025179754 1104 74-75 0.1382714486874228 1104 76-77 0.006389182218203615 1104 78-79 -0.15132717077876734 1104 80-81 -0.2571699761014692 1104 82-83 -0.2323345128653962 1104 84-85 -0.4113859383988192 1104 86-87 -0.5055742766470672 1104 88-89 -0.4529138257392624 1104 90-91 -1.02075241969251 1104 92-93 -4.60814069801223 1104 94-95 -2.2217860720520584 1104 96-97 -1.2734787535108403 1104 98-99 -0.9013225357193058 1104 100-101 -0.9415844964175708 1105 1 0.8901795406267681 1105 2 0.8053210897899135 1105 3 0.8827933764098148 1105 4 0.6816734971733496 1105 5 0.8288039810904735 1105 6 0.6380038626227744 1105 7 0.8677246495165605 1105 8 0.7088494142317288 1105 9 0.9673635705362642 1105 10-11 0.6810055248657108 1105 12-13 0.7658180445530149 1105 14-15 0.9323335495564677 1105 16-17 0.7816414071387143 1105 18-19 0.691297740544293 1105 20-21 0.6911263983081355 1105 22-23 0.7860135428030546 1105 24-25 0.8260849941342485 1105 26-27 0.8279412226496916 1105 28-29 0.8483091077900795 1105 30-31 0.9643786993078862 1105 32-33 1.0691263740479826 1105 34-35 0.8967787870887634 1105 36-37 1.0086784736104661 1105 38-39 1.1245681408048966 1105 40-41 0.8127562788221354 1105 42-43 0.847457675379637 1105 44-45 0.599014987939448 1105 46-47 0.7706849438071472 1105 48-49 0.66676141708286 1105 50-51 0.47798221045938405 1105 52-53 0.5566182972405826 1105 54-55 0.6923852766186798 1105 56-57 0.6000613656131542 1105 58-59 0.7304209961321497 1105 60-61 0.47144535715797886 1105 62-63 0.6479201243982118 1105 64-65 0.9263048652222565 1105 66-67 0.8755711491481755 1105 68-69 0.7352223071363682 1105 70-71 0.7309123616318196 1105 72-73 0.7815887100429642 1105 74-75 0.9656135746998977 1105 76-77 0.6510245467809241 1105 78-79 0.4334397515446007 1105 80-81 0.30054587513354747 1105 82-83 0.128050540826667 1105 84-85 0.2362547688799701 1105 86-87 -0.057666797156223026 1105 88-89 -0.27151508438884164 1105 90-91 -1.4253705759931883 1105 92-93 -7.4553747802399855 1105 94-95 -3.383567758264359 1105 96-97 -1.6696339301695176 1105 98-99 -1.2995739467136858 1105 100-101 -1.0540203355190236 1106 1 0.8194679128499232 1106 2 0.8696326839928119 1106 3 0.7552298656941296 1106 4 0.9266304106085244 1106 5 0.8605103769273086 1106 6 0.65073398404855 1106 7 0.9429302899395964 1106 8 0.8145653786433442 1106 9 1.1051951132591142 1106 10-11 0.721514589831294 1106 12-13 0.8394953709477377 1106 14-15 0.7135992873439463 1106 16-17 0.8836624887793647 1106 18-19 0.7695267848059544 1106 20-21 0.7577776769159357 1106 22-23 0.7459923492492493 1106 24-25 0.7942526959975993 1106 26-27 0.7333011633357174 1106 28-29 0.8081550173642498 1106 30-31 0.6405667273980882 1106 32-33 0.7055555050423337 1106 34-35 0.6198916515059736 1106 36-37 0.9068270103459568 1106 38-39 0.8555498989605681 1106 40-41 0.890429254592938 1106 42-43 0.9373851416657999 1106 44-45 0.8360173241265727 1106 46-47 0.8299377136577348 1106 48-49 0.640099856453908 1106 50-51 0.5701479398533706 1106 52-53 0.4495051670296206 1106 54-55 0.6078908442981543 1106 56-57 0.6269415066237265 1106 58-59 0.5790532075361021 1106 60-61 0.6320284946432899 1106 62-63 0.6090547839024367 1106 64-65 0.7884680765702505 1106 66-67 0.6063011027042187 1106 68-69 0.5284714957659915 1106 70-71 0.38339578449567924 1106 72-73 0.6897587201151474 1106 74-75 0.4832696236059455 1106 76-77 0.3535961012546558 1106 78-79 0.1773264954313447 1106 80-81 0.12324396141858784 1106 82-83 0.23832871496353292 1106 84-85 0.049702533414691885 1106 86-87 0.09111125107834894 1106 88-89 0.23783840730160577 1106 90-91 -0.3006153860206062 1106 92-93 -3.649556345345463 1106 94-95 -1.5603992869980594 1106 96-97 -0.8290944388148098 1106 98-99 -0.7600353786425096 1106 100-101 -0.8419274196217597 1107 1 0.5528201201259684 1107 2 0.49254192155436627 1107 3 0.5342398040583731 1107 4 0.45597451528375643 1107 5 0.3086437497295549 1107 6 0.36253633657938167 1107 7 0.3773889462525588 1107 8 0.2657570185767071 1107 9 0.40542131373366175 1107 10-11 0.29282166486353134 1107 12-13 0.3401943952394717 1107 14-15 0.24243626237296922 1107 16-17 0.41338679727022765 1107 18-19 0.46595590942215637 1107 20-21 0.40503057168539414 1107 22-23 0.3394913659684633 1107 24-25 0.4923338642774482 1107 26-27 0.453755985197823 1107 28-29 0.29797642626678567 1107 30-31 0.2514010876115904 1107 32-33 0.16154365060000941 1107 34-35 0.16399242573919537 1107 36-37 0.23711785264011098 1107 38-39 0.16974711961474043 1107 40-41 0.26482675130003486 1107 42-43 0.11454936332505383 1107 44-45 0.2886975100435123 1107 46-47 0.2977408613036019 1107 48-49 0.32398211173516955 1107 50-51 0.49410818065963724 1107 52-53 0.5126876981343429 1107 54-55 0.41060479105183845 1107 56-57 0.4580122615355471 1107 58-59 0.3705215257014416 1107 60-61 0.17652926451657436 1107 62-63 0.3385828664934323 1107 64-65 0.2843036185564536 1107 66-67 0.07604083734461398 1107 68-69 0.03444991751428006 1107 70-71 -0.005986704845422963 1107 72-73 0.21587718766573616 1107 74-75 0.2720141277891912 1107 76-77 -0.14997431518827753 1107 78-79 -0.2984999423156225 1107 80-81 -0.32831224965748973 1107 82-83 -0.24172614567907402 1107 84-85 -0.350524298103533 1107 86-87 -0.6634021454052466 1107 88-89 -0.8631852315945494 1107 90-91 -1.1715434332731576 1107 92-93 -4.517022114094345 1107 94-95 -2.461765582611047 1107 96-97 -1.6753657451440631 1107 98-99 -1.109231572416519 1107 100-101 -1.0378346424329568 1108 1 0.15649003476971757 1108 2 0.2936368106561069 1108 3 0.05080098633472829 1108 4 -0.3653071181278875 1108 5 -0.9782100387890651 1108 6 -0.2609661192887458 1108 7 -0.03841664567717373 1108 8 0.01426356090848202 1108 9 0.27645818406109157 1108 10-11 0.24127983891059301 1108 12-13 0.19245966040141838 1108 14-15 0.282386303263344 1108 16-17 0.4444959188491495 1108 18-19 0.26122124897704424 1108 20-21 0.22342542420845035 1108 22-23 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0.4378120677644368 2311 70-71 0.5772614090355219 2311 72-73 0.6432692049685009 2311 74-75 0.6372154992735872 2311 76-77 0.5897056785139334 2311 78-79 0.5998764599575352 2311 80-81 0.4896842315549961 2311 82-83 0.7176296633859529 2311 84-85 0.5188888445400792 2311 86-87 0.7053093087199436 2311 88-89 0.9465980120028874 2311 90-91 1.7381062851304847 2311 92-93 2.6522768306983338 2311 94-95 1.814850451968244 2311 96-97 1.5220389661354616 2311 98-99 1.3108825396472525 2311 100-101 1.2345161301687408 2312 1 0.12344224361877565 2312 2 -0.010927667922992157 2312 3 -0.10606689604047048 2312 4 -0.08337694504510296 2312 5 0.09318514592422389 2312 6 0.15304179259710793 2312 7 0.15809093652599415 2312 8 -0.684327939934235 2312 9 -0.11646923115436891 2312 10-11 -0.15834019712141867 2312 12-13 -0.13610958156274222 2312 14-15 -0.2835901196259343 2312 16-17 0.16733835445757705 2312 18-19 -0.025252562252013888 2312 20-21 0.16201806678780173 2312 22-23 0.06663343001921618 2312 24-25 0.07504532878825643 2312 26-27 0.011138478944374697 2312 28-29 -0.07386715959811596 2312 30-31 0.032314311810324625 2312 32-33 0.03952495796836786 2312 34-35 -0.16527326180397495 2312 36-37 -0.04519873888625625 2312 38-39 -0.002837657317961373 2312 40-41 0.07506768866094404 2312 42-43 0.1256869579122366 2312 44-45 -0.02296815679095232 2312 46-47 0.17436466712823062 2312 48-49 0.0786175089114991 2312 50-51 0.21801199470743882 2312 52-53 0.05568894392442658 2312 54-55 0.007663952384042716 2312 56-57 0.07338079248087581 2312 58-59 0.18184527254141258 2312 60-61 0.44063663719629886 2312 62-63 -0.013079900115570808 2312 64-65 0.14945997312887016 2312 66-67 0.25904091701285026 2312 68-69 0.05327474314262304 2312 70-71 0.12144465292503526 2312 72-73 0.34898987097493617 2312 74-75 0.19429424282308716 2312 76-77 0.37055646628174443 2312 78-79 0.3494721240397247 2312 80-81 0.5279340795090519 2312 82-83 0.6987922155879893 2312 84-85 0.800174206370329 2312 86-87 0.6396443981869737 2312 88-89 0.9353995136719142 2312 90-91 1.2389268078753126 2312 92-93 2.601028245782416 2312 94-95 1.9642970145081797 2312 96-97 1.7189524518103987 2312 98-99 1.8852136413444214 2312 100-101 1.7603547774742037 2313 1 -0.3492334079928341 2313 2 -0.9143148591678809 2313 3 -0.45482406273629294 2313 4 -0.28914612289708685 2313 5 -0.7589846160736613 2313 6 -0.31817865368878984 2313 7 -1.0381229744802027 2313 8 -1.132174688116642 2313 9 -1.3278273015860123 2313 10-11 -1.8550417972330635 2313 12-13 -0.5832685284097963 2313 14-15 -0.4432350868709136 2313 16-17 -1.452947153731671 2313 18-19 -1.0679442768931935 2313 20-21 -1.3681375809714282 2313 22-23 -1.1110715124211836 2313 24-25 -0.39557134118699366 2313 26-27 -0.9213751763505797 2313 28-29 -2.3175280611325704 2313 30-31 -0.6619338558544925 2313 32-33 -0.35637245877806834 2313 34-35 -0.03635049701705384 2313 36-37 0.04350552152646259 2313 38-39 -0.3549145495516939 2313 40-41 -0.2041440288498677 2313 42-43 -0.0347252904794928 2313 44-45 -0.66463434846235 2313 46-47 -0.8920605545307687 2313 48-49 -0.5130364721057248 2313 50-51 -0.6020315852892999 2313 52-53 -0.3339230570111482 2313 54-55 -0.16117254179710017 2313 56-57 -0.50754083428291 2313 58-59 -0.5333092794528795 2313 60-61 -0.41857427446047524 2313 62-63 -0.38922013444220127 2313 64-65 -0.4521471651655098 2313 66-67 -0.6915929068431801 2313 68-69 -0.5284461041047948 2313 70-71 -0.5228788915748162 2313 72-73 -0.3582530615868684 2313 74-75 -0.4026988897281072 2313 76-77 -0.1767678034867437 2313 78-79 -0.013403826524079676 2313 80-81 -0.23111114069547511 2313 82-83 -0.18802779592982688 2313 84-85 0.04554349389708534 2313 86-87 0.015420769760375208 2313 88-89 0.3546964256785472 2313 90-91 1.0187153989476272 2313 92-93 2.2199554722490227 2313 94-95 1.5050764227754563 2313 96-97 1.005132841254106 2313 98-99 0.9286389779850346 2313 100-101 1.0443315983603636 2314 1 -0.6160311097140969 2314 2 -0.6416475957751864 2314 3 -1.6738420542298904 2314 4 -0.5377611815978014 2314 5 -0.4417351900316717 2314 6 -2.9805674811833143 2314 7 -3.968634806374734 2314 8 -1.5913517974887768 2314 9 -1.8730471387165082 2314 10-11 -1.2415785570515183 2314 12-13 -2.282700840104674 2314 14-15 -2.9246744777210196 2314 16-17 -2.158199837979332 2314 18-19 -2.809727655150894 2314 20-21 -2.267945717922096 2314 22-23 -3.2862783106122535 2314 24-25 -1.9097732337192213 2314 26-27 -2.185060455703997 2314 28-29 -1.2360120066198164 2314 30-31 -1.4134426619810583 2314 32-33 -2.252379015967932 2314 34-35 -0.7587518333299386 2314 36-37 -0.2865491319170843 2314 38-39 -0.424263051144095 2314 40-41 -0.6907829389209468 2314 42-43 -0.6112189575716158 2314 44-45 -1.263217497952418 2314 46-47 -1.586688898881107 2314 48-49 -1.7492795754344748 2314 50-51 -0.8992849974339663 2314 52-53 -0.46228728688795684 2314 54-55 -0.2693717816850416 2314 56-57 -0.3938474501938849 2314 58-59 -0.47309236818291467 2314 60-61 -0.5114474083323586 2314 62-63 -0.5520590551156985 2314 64-65 -0.48061007911870135 2314 66-67 -0.4314915517721616 2314 68-69 -0.42677882236176146 2314 70-71 -0.5345517497837413 2314 72-73 -0.4243629821910453 2314 74-75 -0.22524035131267794 2314 76-77 -0.1318267896505816 2314 78-79 -0.07777046437390567 2314 80-81 -0.05673393100812518 2314 82-83 0.22021589257103003 2314 84-85 -0.051783676438969195 2314 86-87 -0.04749389705852991 2314 88-89 0.16521739196692664 2314 90-91 0.9437032673308003 2314 92-93 1.73708730316487 2314 94-95 1.1002693460669413 2314 96-97 1.0671172475388921 2314 98-99 1.1615418088315579 2314 100-101 1.4834048855595707 2315 1 -7.532253746404852 2315 2 -3.0433724951547525 2315 3 -1.4107423660893623 2315 4 -0.8843209508395233 2315 5 -1.1798942023845598 2315 6 -1.5732761952596448 2315 7 -3.0189431984239583 2315 8 -2.5579821561558447 2315 9 -3.0143443510651196 2315 10-11 -2.165405538396783 2315 12-13 -2.6806920031303676 2315 14-15 -4.5150777467966705 2315 16-17 -4.061141985205573 2315 18-19 -2.988195899034288 2315 20-21 -4.005820166979923 2315 22-23 -5.125358353848142 2315 24-25 -3.082296803068452 2315 26-27 -4.395199694883679 2315 28-29 -2.66458088397264 2315 30-31 -2.213958056555505 2315 32-33 -1.453331540434121 2315 34-35 -2.1360585166958472 2315 36-37 -2.798796564069008 2315 38-39 -1.924496385224785 2315 40-41 -3.0487239364228387 2315 42-43 -7.0115331634197275 2315 44-45 -3.644567900043093 2315 46-47 -2.2886065661034287 2315 48-49 -5.049425937169563 2315 50-51 -6.382101702172758 2315 52-53 -6.770552240734737 2315 54-55 -4.306155270079081 2315 56-57 -3.4933377129932346 2315 58-59 -3.8872471956434396 2315 60-61 -2.6133331909087936 2315 62-63 -2.7961598742095717 2315 64-65 -1.683602647363056 2315 66-67 -2.405365173862723 2315 68-69 -1.6555653652162334 2315 70-71 -1.3977340303675838 2315 72-73 -1.7359822112135284 2315 74-75 -2.3499930041517167 2315 76-77 -2.379724697256542 2315 78-79 -1.4227994838337068 2315 80-81 -1.1405893765041526 2315 82-83 -1.7075947141966488 2315 84-85 -1.5744163001412268 2315 86-87 -2.0365554821347445 2315 88-89 -1.6698586905858903 2315 90-91 -0.7609474067830924 2315 92-93 0.23539765157045167 2315 94-95 -0.01194388113371403 2315 96-97 -0.20160109919398295 2315 98-99 -0.09624125147917795 2315 100-101 -0.14634202908603733 2316 1 -4.156770825888817 2316 2 -2.9099393880792626 2316 3 -4.606234098269834 2316 4 -4.249000220022104 2316 5 -4.23124637982945 2316 6 -4.501788538473974 2316 7 -3.0828354758261725 2316 8 -1.7843535402755748 2316 9 -1.8479355173715177 2316 10-11 -3.0466160407993357 2316 12-13 -4.044130755178394 2316 14-15 -3.122391703647054 2316 16-17 -3.007452376085503 2316 18-19 -3.518547539518373 2316 20-21 -5.932357458219197 2316 22-23 -6.586045939039039 2316 24-25 -4.400251808506901 2316 26-27 -4.099694332159775 2316 28-29 -5.015730117770886 2316 30-31 -6.633543632962272 2316 32-33 -4.88079584630902 2316 34-35 -6.307045285430963 2316 36-37 -9.525357674338728 2316 38-39 -6.453143794733123 2316 40-41 -5.572892817479136 2316 42-43 -4.745396389865732 2316 44-45 -3.5451425857833385 2316 46-47 -6.065523998053973 2316 48-49 -3.620451945347888 2316 50-51 -4.7025885466331125 2316 52-53 -5.546226814952359 2316 54-55 -7.067874921467606 2316 56-57 -5.271977412295186 2316 58-59 -6.081408504175613 2316 60-61 -6.376541325176529 2316 62-63 -6.78664341429576 2316 64-65 -5.607376518024342 2316 66-67 -7.024329527926412 2316 68-69 -5.825954179909687 2316 70-71 -6.570444305594414 2316 72-73 -8.408135424028995 2316 74-75 -8.160795691709371 2316 76-77 -7.547901646493095 2316 78-79 -6.6707816126767625 2316 80-81 -6.547949732275104 2316 82-83 -5.40726813188331 2316 84-85 -7.01556773685558 2316 86-87 -5.5439563164892185 2316 88-89 -5.39325393504074 2316 90-91 -5.751291061696282 2316 92-93 -4.503576615615735 2316 94-95 -4.5202078455566195 2316 96-97 -4.93784443881481 2316 98-99 -5.787771865128995 2316 100-101 -4.839292284486625 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 8612.0 3 3237.0 4 3403.0 5 3958.0 6 4612.0 7 5644.0 8 6837.0 9 8622.0 10 10593.0 11 13135.0 12 15881.0 13 18606.0 14 20076.0 15 23100.0 16 26412.0 17 29434.0 18 25534.0 19 23258.0 20 24900.0 21 27043.0 22 29751.0 23 33363.0 24 37875.0 25 42969.0 26 48640.0 27 55083.0 28 63437.0 29 71993.0 30 82783.0 31 95699.0 32 110246.0 33 128052.0 34 149736.0 35 177327.0 36 198232.0 37 166079.0 38 48399.0 39 697.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.08694500071676 19.304646333887916 13.356961030047392 22.251447635347933 2 15.097063099296145 21.475037620198638 40.706711247937065 22.721188032568147 3 18.88496875629585 27.630001992950277 30.291432323046923 23.19359692770695 4 11.984607882215327 16.61163792682119 38.74008803874313 32.66366615222036 5 12.485901507948101 39.33388324466511 34.30029394371681 13.87992130366998 6 29.636806582252916 37.36022512747034 18.63181744390409 14.371150846372654 7 26.75994235341009 34.38383419328984 22.137463441440598 16.71876001185947 8 25.759750220593226 34.38045204642639 20.879657910812462 18.98013982216792 9 27.66577239732056 15.95252716607836 20.861560915379968 35.52013952122112 10-11 24.37676950761669 26.851935313263258 28.209087246295912 20.56220793282414 12-13 26.174304406765486 24.661200258577882 28.375067162645323 20.789428172011306 14-15 23.25239158418678 25.304105137660166 27.498554854047725 23.944948424105334 16-17 21.239093509647233 28.856830389820825 27.85786972377827 22.04620637675367 18-19 21.204805145297975 27.297496749978006 30.73502627712017 20.762671827603846 20-21 22.52790072501154 26.37010888750102 30.164444565128846 20.937545822358597 22-23 21.89749484774891 26.51940828341305 30.011397547938547 21.571699320899494 24-25 21.712002372899402 26.58788148106298 30.513785958824112 21.186330187213507 26-27 21.656915349475998 26.38986489588288 30.66763224581631 21.285587508824808 28-29 21.355553569590175 26.65105480567253 30.752876156255155 21.24051546848214 30-31 21.37010079223977 26.931778824801327 30.49385616902564 21.20426421393326 32-33 21.327813791520494 26.938949575780295 30.231171558061803 21.502065074637404 34-35 21.74219378109993 26.717612611633818 30.184209382673128 21.35598422459312 36-37 21.999833763231035 26.439224706478022 30.28383026899934 21.277111261291605 38-39 21.84638107700991 26.69764076456372 30.27804983776895 21.17792832065742 40-41 21.6279645174546 26.795807516887237 30.37467106183372 21.201556903824443 42-43 21.325009023856385 27.155031346527764 29.8465985153889 21.67336111422695 44-45 21.45773044468198 27.218521608398294 29.610441596927117 21.713306349992614 46-47 21.76601398476034 27.176491391329645 29.00084447461266 22.05665014929735 48-49 21.822687573231793 27.205185401916733 28.96576399184015 22.006363033011326 50-51 21.938227821688322 27.604521993626562 28.867472026895697 21.589778157789418 52-53 21.851363762342878 27.682283316939564 28.786362920011086 21.67999000070647 54-55 21.56933656052229 27.593307894041835 28.829859612932957 22.007495932502916 56-57 21.61679541923357 28.09560285537338 28.239566696002694 22.048035029390352 58-59 21.67850021850945 27.481399536037493 28.698398022903703 22.141702222549362 60-61 21.649216017677798 27.58907272780111 28.896411560639635 21.86529969388146 62-63 21.970893812796458 27.22972486681957 28.953146833205007 21.846234487178965 64-65 21.748705581282053 27.524418601177832 28.80915650362073 21.917719313919385 66-67 21.38142162223967 27.780386541891396 28.59762527929543 22.2405665565735 68-69 21.547544103692495 27.773634630937956 28.69929752387077 21.97952374149878 70-71 21.68137735140316 27.64350284956425 28.364592123778888 22.310527675253702 72-73 21.581827888192937 27.52177852399135 28.09021396134813 22.80617962646758 74-75 21.488090822709914 28.023404531082 28.030903893228647 22.457600752979435 76-77 21.60091366188641 27.541427816798077 28.26963460046042 22.5880239208551 78-79 21.43994104722689 27.63416674048444 28.38248872218451 22.54340349010416 80-81 21.47052397712179 28.0508357590527 27.960971823974294 22.517668439851217 82-83 21.447554275608734 27.77432368463816 27.99717609769963 22.78094594205348 84-85 21.410999993751517 27.558502787231248 27.87494773686711 23.15554948215012 86-87 21.31583022491965 27.453156454859855 28.267148799061093 22.9638645211594 88-89 21.65797386889011 27.09400232278948 28.14352382489624 23.104499983424173 90-91 21.070922089981412 27.296983701904953 28.537060881362425 23.09503332675121 92-93 20.6743181244201 27.393498788243427 28.714212543685147 23.21797054365133 94-95 21.111579096106606 27.36519314797504 27.99998913067107 23.523238625247277 96-97 21.124499170708493 26.98370251276821 27.941240015686287 23.95055830083701 98-99 21.170621788346512 27.016391749354778 27.795057604203333 24.017928858095374 100-101 21.270772148168767 27.02651789044245 27.949528151609094 23.753181809779694 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3031.0 1 1999.5 2 1122.5 3 1299.5 4 1146.5 5 1098.0 6 1590.5 7 2798.0 8 4115.0 9 4394.5 10 3631.0 11 3005.0 12 2941.0 13 3023.5 14 3140.0 15 3276.0 16 3492.0 17 3716.0 18 3955.5 19 4180.0 20 4626.0 21 5273.5 22 6071.5 23 7075.0 24 8107.5 25 9579.5 26 11859.5 27 14827.5 28 17617.5 29 20387.0 30 23084.5 31 26414.5 32 30355.0 33 33714.5 34 37250.0 35 40936.5 36 45550.5 37 50562.5 38 53937.0 39 55552.0 40 56207.5 41 56931.5 42 57280.0 43 58293.5 44 60848.0 45 62823.5 46 64367.5 47 67542.5 48 76615.0 49 76999.0 50 67457.5 51 62920.5 52 60392.5 53 58398.0 54 55762.5 55 51797.5 56 47378.0 57 42521.5 58 38517.5 59 34944.0 60 30017.0 61 24877.5 62 20043.0 63 14997.0 64 10677.5 65 7712.5 66 5466.0 67 3933.5 68 2849.0 69 2004.5 70 1410.0 71 965.5 72 625.0 73 435.5 74 318.0 75 228.5 76 165.5 77 105.0 78 63.0 79 40.0 80 21.5 81 11.5 82 13.0 83 11.0 84 5.5 85 6.0 86 7.0 87 6.0 88 5.0 89 2.5 90 1.0 91 1.5 92 2.5 93 3.0 94 2.5 95 1.5 96 0.5 97 1.0 98 1.0 99 0.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08864738414264309 2 0.09911797480331022 3 0.09586286889843962 4 0.09743617008579374 5 0.09488633712697842 6 0.07904482172327476 7 0.09179398651735135 8 0.0885931323775619 9 0.08305945233928186 10-11 0.08918990179345485 12-13 0.08932553120615778 14-15 0.09485921124443784 16-17 0.0919024900475137 18-19 0.09396405712059841 20-21 0.10351236777488555 22-23 0.11243678313073917 24-25 0.13411036328066933 26-27 0.13923715508084056 28-29 0.13907439978559702 30-31 0.1405391974427888 32-33 0.1370128327125123 34-35 0.13118076796628578 36-37 0.13617193035375405 38-39 0.12830542441698342 40-41 0.12458917850892279 42-43 0.1250231926295722 44-45 0.11512224550225743 46-47 0.1339204821028852 48-49 0.15100978810345594 50-51 0.16077510581806778 52-53 0.1696995211739214 54-55 0.16601040114840135 56-57 0.13660594447440347 58-59 0.12892931971541693 60-61 0.1250231926295722 62-63 0.1215510796643769 64-65 0.10782538309883911 66-67 0.12160533142945805 68-69 0.11929963141350804 70-71 0.12816979500428047 72-73 0.1549159151893007 74-75 0.16826184939927022 76-77 0.17306313060895434 78-79 0.1704047941199767 80-81 0.17653524357414968 82-83 0.17637248827890617 84-85 0.1525288375257289 86-87 0.15315273282416245 88-89 0.17517894944712026 90-91 0.2682749783264199 92-93 0.5408358460942526 94-95 0.1745821800312273 96-97 0.1863276871713021 98-99 0.20428502141317167 100-101 0.21136487675626528 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1843258.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.00666696370568 #Duplication Level Percentage of deduplicated Percentage of total 1 75.06524131414693 38.28827764260893 2 12.489550745400546 12.741007107938954 3 4.834815845138511 7.398235249314819 4 2.3968269976232564 4.890166257495522 5 1.3098591214426965 3.3405773988399874 6 0.8611167716291468 2.6353617832409575 7 0.5800971644293165 2.0712176010845313 8 0.42088599853731457 1.7174393565663617 9 0.31417706461502 1.4422612412207667 >10 1.5814075765738753 14.257477570551593 >50 0.09525217312104635 3.373701090637029 >100 0.04643972998812285 4.207043991292692 >500 0.00227298611095199 0.779210050875182 >1k 0.0017317989416777066 1.5547211451534646 >5k 2.1647486770971333E-4 0.6978330057433967 >10k+ 1.0823743385485666E-4 0.6054695074357461 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10967 0.5949791076452673 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7027 0.38122715322543016 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5613 0.30451515740064605 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 4527 0.2455977405224879 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 3597 0.19514359899699335 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 5.425176508117692E-5 5.425176508117692E-5 10-11 0.0 0.0 0.0 8.137764762176538E-5 5.425176508117692E-5 12-13 0.0 0.0 0.0 1.0850353016235384E-4 5.425176508117692E-5 14-15 0.0 0.0 0.0 1.6275529524353076E-4 5.425176508117692E-5 16-17 0.0 0.0 0.0 4.8826588573059227E-4 5.425176508117692E-5 18-19 0.0 0.0 0.0 6.238952984335346E-4 5.425176508117692E-5 20-21 0.0 0.0 0.0 8.951541238394191E-4 5.425176508117692E-5 22-23 0.0 0.0 0.0 0.002305700015950019 5.425176508117692E-5 24-25 0.0 0.0 0.0 0.0038247494382229724 1.0850353016235382E-4 26-27 0.0 0.0 0.0 0.005018288270008865 1.6275529524353074E-4 28-29 0.0 0.0 0.0 0.007595247111364768 1.6275529524353074E-4 30-31 0.0 0.0 0.0 0.017469068356138966 2.1700706032470765E-4 32-33 0.0 0.0 0.0 0.036891200255200304 3.255105904870615E-4 34-35 0.0 0.0 0.0 0.06572601339584583 3.255105904870615E-4 36-37 0.0 0.0 0.0 0.11167725841960269 3.255105904870615E-4 38-39 0.0 0.0 0.0 0.17539595650744497 3.255105904870615E-4 40-41 0.0 0.0 0.0 0.25718049236731916 3.255105904870615E-4 42-43 0.0 0.0 0.0 0.3364965729159998 3.255105904870615E-4 44-45 0.0 0.0 0.0 0.43054200768422 3.255105904870615E-4 46-47 0.0 0.0 0.0 0.5417852519831733 3.5263647302764996E-4 48-49 0.0 0.0 0.0 0.6669983257905296 4.8826588573059227E-4 50-51 0.0 0.0 0.0 0.8050419420395842 4.8826588573059227E-4 52-53 0.0 0.0 0.0 0.9395591935583625 4.8826588573059227E-4 54-55 0.0 0.0 0.0 1.0788506004042842 4.8826588573059227E-4 56-57 0.0 0.0 0.0 1.2429893156573848 4.8826588573059227E-4 58-59 0.0 0.0 0.0 1.4311886887239877 4.8826588573059227E-4 60-61 0.0 0.0 0.0 1.6593716126554177 4.8826588573059227E-4 62-63 0.0 0.0 0.0 1.8813969612501342 4.8826588573059227E-4 64-65 0.0 0.0 0.0 2.097753000393868 4.8826588573059227E-4 66-67 0.0 0.0 0.0 2.332880150255688 4.8826588573059227E-4 68-69 0.0 0.0 0.0 2.6070956968584973 4.8826588573059227E-4 70-71 0.0 2.712588254058846E-5 0.0 2.9171716601799638 5.425176508117691E-4 72-73 0.0 5.425176508117692E-5 0.0 3.242573747136863 7.052729460552999E-4 74-75 0.0 5.425176508117692E-5 0.0 3.568301344684249 7.052729460552999E-4 76-77 0.0 5.425176508117692E-5 0.0 3.9001593916858086 7.052729460552999E-4 78-79 0.0 5.425176508117692E-5 0.0 4.2781856907714495 7.052729460552999E-4 80-81 0.0 5.425176508117692E-5 0.0 4.703790787833283 7.323988285958883E-4 82-83 0.0 5.425176508117692E-5 0.0 5.135526334349288 8.137764762176537E-4 84-85 0.0 5.425176508117692E-5 0.0 5.56568857967794 8.137764762176537E-4 86-87 0.0 5.425176508117692E-5 0.0 6.02162041342015 8.137764762176537E-4 88-89 0.0 5.425176508117692E-5 0.0 6.499361456724994 8.137764762176537E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 11045 0.0 30.460262 1 TACATGG 11280 0.0 29.573269 2 ACATGGG 11300 0.0 28.051182 3 CATGGGA 7550 0.0 25.201532 4 GTATCAA 16615 0.0 24.475615 1 CATGGGG 3910 0.0 23.66396 4 GAGTACT 7965 0.0 22.728653 12-13 ATGGGAG 2890 0.0 21.836515 5 GGTATCA 12280 0.0 21.407429 1 ATGGGAT 3105 0.0 21.24138 5 AGTACTT 8440 0.0 21.14026 12-13 TATCAAC 19250 0.0 20.977415 2 ATCAACG 19550 0.0 20.728325 3 TCAACGC 19555 0.0 20.697636 4 CAACGCA 19570 0.0 20.633282 5 AACGCAG 19965 0.0 20.390867 6 AGTACAT 7720 0.0 20.283823 2 GTACTTT 9015 0.0 20.213533 14-15 GTATAGG 455 0.0 18.77216 1 GTATATG 860 0.0 18.760033 1 >>END_MODULE