FastQCFastQC Report
Fri 27 May 2016
SRR939637_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939637_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1843258
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT113650.6165713101475756No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83370.4522969654817719No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68510.37167884257114303No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA43490.23594092633803843No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA39140.21234140852772646No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT23970.13004148089958106No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19770.10725573956548676No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA19770.10725573956548676No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA19470.10562818661305146No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT19010.1031326054193173No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG18940.10275284306374907No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18470.10020301010493377No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG108450.028.5472851
TACATGG108900.028.1230622
ACATGGG108300.026.9756953
CATGGGA73350.024.179694
GTATCAA165050.024.031481
CATGGGG38400.023.3997734
GGTATCA122000.022.5613211
ATGGGAG26800.021.9424295
ATGGGAT29400.021.7621145
GAGTACT85800.021.21454212-13
AGTACTT89550.021.19166412-13
GTCGGGA4550.020.678932
TCAACGC192800.020.4965954
ATCAACG193200.020.454163
TATCAAC196350.020.2458152
CAACGCA195700.020.2169045
AACGCAG195650.020.1935446
ATGGGGA19400.019.1573245
GTACTTT95650.019.12809614-15
CGCAGAG211600.018.3605738
Produced by FastQC (version 0.11.5)