##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939637_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1843258 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.446745382361016 31.0 31.0 34.0 30.0 34.0 2 31.64513920460402 31.0 31.0 34.0 30.0 34.0 3 31.683114897643193 31.0 31.0 34.0 30.0 34.0 4 35.49516291262536 37.0 35.0 37.0 33.0 37.0 5 35.22969383558894 37.0 35.0 37.0 33.0 37.0 6 35.26177236176379 37.0 35.0 37.0 32.0 37.0 7 35.18426557758057 37.0 35.0 37.0 32.0 37.0 8 35.147712365821825 37.0 35.0 37.0 32.0 37.0 9 36.72754709324468 39.0 35.0 39.0 32.0 39.0 10-11 36.604957906055475 38.5 35.0 39.0 32.0 39.0 12-13 36.53951861323809 38.0 35.0 39.0 32.0 39.0 14-15 37.520210138786865 39.0 36.0 41.0 32.0 41.0 16-17 37.49322422580019 39.0 36.0 41.0 32.0 41.0 18-19 37.51975903536021 39.0 36.0 41.0 32.0 41.0 20-21 37.46418054336398 39.0 36.0 41.0 32.0 41.0 22-23 37.31776750731585 39.0 36.0 40.5 31.5 41.0 24-25 37.17535445390716 39.0 36.0 40.5 31.0 41.0 26-27 36.878093028756695 39.0 36.0 40.0 30.5 41.0 28-29 36.655872102548855 38.5 35.0 40.0 30.0 41.0 30-31 36.386519141650275 38.0 35.0 40.0 30.0 41.0 32-33 36.159116086841884 38.0 35.0 40.0 30.0 41.0 34-35 36.10842459384416 38.0 35.0 40.0 30.0 41.0 36-37 35.956951495666914 38.0 35.0 40.0 29.5 41.0 38-39 35.94916311227186 38.0 35.0 40.0 29.5 41.0 40-41 35.81570078632508 38.0 35.0 40.0 29.0 41.0 42-43 35.59193558362421 38.0 34.5 40.0 28.0 41.0 44-45 35.324442373232614 38.0 34.0 40.0 27.0 41.0 46-47 34.93125568965387 38.0 34.0 40.0 25.5 41.0 48-49 34.561542388531606 38.0 33.0 40.0 24.0 41.0 50-51 34.281910345703096 38.0 33.0 40.0 24.0 41.0 52-53 34.00251429805269 38.0 33.0 40.0 23.0 41.0 54-55 33.69565546440053 37.0 33.0 40.0 23.0 41.0 56-57 31.644732587624738 36.0 29.0 39.5 13.5 40.5 58-59 26.64731036024257 32.0 16.0 37.5 4.0 40.0 60-61 27.35720718423574 32.0 18.0 37.5 2.0 40.0 62-63 30.36415602156616 34.5 28.5 38.0 2.0 40.0 64-65 30.550816000798584 35.0 29.5 38.0 2.0 40.0 66-67 23.973864754689792 26.5 16.5 35.0 2.0 38.5 68-69 16.697660880896763 13.0 7.0 28.0 2.0 34.5 70-71 15.583778288226608 12.0 7.0 25.0 2.0 33.5 72-73 16.633315032404578 13.0 7.0 28.0 2.0 34.0 74-75 17.580407354803288 16.5 7.5 29.0 2.0 34.0 76-77 18.78403158971777 21.0 7.0 30.0 2.0 34.0 78-79 21.77435117601551 26.5 9.0 33.0 2.0 35.5 80-81 25.300191291723678 30.5 20.0 34.5 2.0 36.0 82-83 26.591155171983523 32.5 25.0 35.0 2.0 36.0 84-85 26.650283085710193 33.0 25.0 35.0 2.0 36.0 86-87 26.402839700139644 33.0 25.0 35.0 2.0 35.5 88-89 25.96142943635671 33.0 23.5 35.0 2.0 35.0 90-91 24.57982197825806 32.0 11.0 34.5 2.0 35.0 92-93 22.06880073218182 30.5 2.0 34.0 2.0 35.0 94-95 15.00436997967729 4.5 2.0 31.5 2.0 34.0 96-97 12.550224114041551 2.0 2.0 28.5 2.0 34.0 98-99 12.192416362766362 2.0 2.0 29.0 2.0 34.0 100-101 11.899034481336852 2.0 2.0 29.5 2.0 34.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.3836095959703627 1101 2 0.4525442527549366 1101 3 0.4591799033029922 1101 4 0.301037237529755 1101 5 0.4696768672406222 1101 6 0.5735182291501459 1101 7 0.5688683812654958 1101 8 0.5227042987889448 1101 9 0.5406954888358939 1101 10-11 0.6092255950760688 1101 12-13 0.5592014206346079 1101 14-15 0.7281718438481377 1101 16-17 0.706857135184606 1101 18-19 0.5854478459244703 1101 20-21 0.6217246869422226 1101 22-23 0.683034785360789 1101 24-25 0.6678208235343703 1101 26-27 0.7240383417835403 1101 28-29 0.5699574557956382 1101 30-31 0.5759564203299874 1101 32-33 0.6748297352968464 1101 34-35 0.48369471068981795 1101 36-37 0.5279982382818602 1101 38-39 0.4408433738520259 1101 40-41 0.3555435259345643 1101 42-43 0.3834643496575154 1101 44-45 0.3578981915256705 1101 46-47 0.4442745802921664 1101 48-49 0.5580601258812052 1101 50-51 0.4456083796147041 1101 52-53 0.5028649715284175 1101 54-55 0.3070512562196086 1101 56-57 -1.947035966087224 1101 58-59 -9.917811926911885 1101 60-61 -10.191967341918755 1101 62-63 -3.9960633796797715 1101 64-65 -5.349110552280415 1101 66-67 -9.815067686030268 1101 68-69 -5.036630322175821 1101 70-71 -4.322767755302969 1101 72-73 -4.642380032149575 1101 74-75 -6.664695354555086 1101 76-77 -7.839902250986668 1101 78-79 -10.74757671667921 1101 80-81 -7.843335238141716 1101 82-83 -4.212030593362446 1101 84-85 -3.467636334796879 1101 86-87 -3.399188703708795 1101 88-89 -3.486010980108855 1101 90-91 -9.349914983861508 1101 92-93 -16.401314289200762 1101 94-95 -10.908256255800413 1101 96-97 -8.880054595001049 1101 98-99 -8.660689638696368 1101 100-101 -8.603237879936163 1102 1 0.2922323979861119 1102 2 0.35342961082795554 1102 3 0.29483001349115767 1102 4 0.2796938665423738 1102 5 0.18995958079761976 1102 6 0.18517120577175916 1102 7 0.3478147358094077 1102 8 0.33508659779185734 1102 9 0.3767177730443123 1102 10-11 0.4235554506756216 1102 12-13 0.43103640036923707 1102 14-15 0.3924088485793007 1102 16-17 0.24013203539881545 1102 18-19 0.42031854820066883 1102 20-21 0.3566924780932723 1102 22-23 0.5426906977650461 1102 24-25 0.4599387094626479 1102 26-27 0.5165663511533864 1102 28-29 0.4392760228871353 1102 30-31 0.3795151899211362 1102 32-33 0.2929046401343456 1102 34-35 0.17060736152431843 1102 36-37 0.34175769674168777 1102 38-39 0.35907701129442415 1102 40-41 0.409054328998252 1102 42-43 0.46238758780265954 1102 44-45 0.37916990167685327 1102 46-47 0.3288110579694319 1102 48-49 0.4484003607438183 1102 50-51 0.5235690123875969 1102 52-53 0.22279573791409035 1102 54-55 0.10110167308533136 1102 56-57 -2.8899068687055447 1102 58-59 -10.149889397669643 1102 60-61 -10.561570433351818 1102 62-63 -4.562099418145458 1102 64-65 -5.663253357686571 1102 66-67 -6.859178590806113 1102 68-69 -3.992118347881256 1102 70-71 -4.2769612626771 1102 72-73 -4.624649561653804 1102 74-75 -5.170374668460369 1102 76-77 -7.318323478005114 1102 78-79 -9.80915206072828 1102 80-81 -5.6588588631012655 1102 82-83 -3.3569420960824736 1102 84-85 -2.8627982651702837 1102 86-87 -2.7691273681973847 1102 88-89 -2.838225531111334 1102 90-91 -8.421792151023894 1102 92-93 -12.685907979964993 1102 94-95 -10.02484207928359 1102 96-97 -8.745046542788808 1102 98-99 -8.711368297637023 1102 100-101 -8.643280105891257 1103 1 0.3066551215015707 1103 2 0.30074121456459935 1103 3 0.2683938543701423 1103 4 0.28048686130524914 1103 5 0.2902189617936486 1103 6 0.24675896550905208 1103 7 0.18311995478307352 1103 8 0.28261272716431307 1103 9 0.41863548221055424 1103 10-11 0.4982630282311433 1103 12-13 0.4583306620335037 1103 14-15 0.6010659331008981 1103 16-17 0.5556633913958535 1103 18-19 0.5180291246145501 1103 20-21 0.4749912147923041 1103 22-23 0.5297025956868495 1103 24-25 0.612892637457044 1103 26-27 0.5873316076015342 1103 28-29 0.5397382281206546 1103 30-31 0.5280824437079588 1103 32-33 0.5927790751293216 1103 34-35 0.5269072893656954 1103 36-37 0.5814416389727839 1103 38-39 0.5203326313410273 1103 40-41 0.5781873903990942 1103 42-43 0.5350307686627715 1103 44-45 0.5414436933949602 1103 46-47 0.545834492269833 1103 48-49 0.5431797368792672 1103 50-51 0.36989319812779087 1103 52-53 0.4633761872777882 1103 54-55 0.5493640904442074 1103 56-57 -2.1857255690402333 1103 58-59 -9.690781734303872 1103 60-61 -9.711257720982687 1103 62-63 -3.9079670993333586 1103 64-65 -4.952107925807461 1103 66-67 -6.20364075403462 1103 68-69 -3.6219404116178726 1103 70-71 -3.8024983643607513 1103 72-73 -4.403421274873535 1103 74-75 -4.716225097586648 1103 76-77 -6.406256185963716 1103 78-79 -8.901056793343827 1103 80-81 -4.701550064699855 1103 82-83 -2.477691825675162 1103 84-85 -2.009124551161463 1103 86-87 -1.963040390803581 1103 88-89 -2.0198120306980343 1103 90-91 -4.949854054421348 1103 92-93 -9.943953066352815 1103 94-95 -7.98727819039887 1103 96-97 -7.807208421133271 1103 98-99 -8.021560515607069 1103 100-101 -8.083953416351857 1104 1 0.23311193168547106 1104 2 0.23837822781738538 1104 3 0.18467501855932866 1104 4 0.14081268153027793 1104 5 0.26353525126384625 1104 6 0.19003231186209746 1104 7 0.22496082782309657 1104 8 0.08724335393113591 1104 9 0.2530839017570514 1104 10-11 0.30936659660279986 1104 12-13 0.40440428305951315 1104 14-15 0.3483870489376173 1104 16-17 0.3180796358621052 1104 18-19 0.3058968908437194 1104 20-21 0.32282928834123936 1104 22-23 0.43985729381953576 1104 24-25 0.5529570691104624 1104 26-27 0.47324411815055356 1104 28-29 0.5267593037071805 1104 30-31 0.6088922170087372 1104 32-33 0.7121547243628612 1104 34-35 0.50593330781183 1104 36-37 0.5027261294839533 1104 38-39 0.5909320196739003 1104 40-41 0.41422400788877667 1104 42-43 0.61768183813993 1104 44-45 0.6317604527554792 1104 46-47 0.5990356625291966 1104 48-49 0.5027933297954448 1104 50-51 0.39804769931923545 1104 52-53 0.36421274354329825 1104 54-55 0.46359330445796587 1104 56-57 -2.4391681882229896 1104 58-59 -9.579624594778831 1104 60-61 -9.941733569168672 1104 62-63 -3.939997999780605 1104 64-65 -5.095876472305314 1104 66-67 -6.208176018402124 1104 68-69 -4.055965877635973 1104 70-71 -3.9421432902635676 1104 72-73 -4.307675310275421 1104 74-75 -5.035626970827009 1104 76-77 -6.7103488810367615 1104 78-79 -8.892930877364606 1104 80-81 -5.003612619403942 1104 82-83 -2.9513902438503834 1104 84-85 -2.462121545064903 1104 86-87 -2.3083419969195234 1104 88-89 -2.233368039978828 1104 90-91 -4.682381591587479 1104 92-93 -6.8059296407922965 1104 94-95 -7.6548796314655085 1104 96-97 -7.311242059951905 1104 98-99 -7.659805695966332 1104 100-101 -7.667112510662422 1105 1 0.28577658924988825 1105 2 0.2575563761813804 1105 3 0.27485399902823815 1105 4 0.25118449010667376 1105 5 0.20912145930670079 1105 6 0.12175451971380369 1105 7 0.2602081582830209 1105 8 0.1641899444305963 1105 9 0.31425036533195083 1105 10-11 0.41995344352770303 1105 12-13 0.39056677309970667 1105 14-15 0.4373037179416528 1105 16-17 0.39389558692893445 1105 18-19 0.48852548014021835 1105 20-21 0.46682577085405086 1105 22-23 0.5473499778409447 1105 24-25 0.44741596198501554 1105 26-27 0.46202147970273444 1105 28-29 0.45363691572190135 1105 30-31 0.5336185365598851 1105 32-33 0.5069804809706184 1105 34-35 0.42442416452668397 1105 36-37 0.24196771935491768 1105 38-39 0.4737433915666216 1105 40-41 0.3712410104619721 1105 42-43 0.0834690550970052 1105 44-45 0.10793002243985228 1105 46-47 0.26342585740380997 1105 48-49 -0.011979648921993657 1105 50-51 0.3084731895703072 1105 52-53 0.36163604912942304 1105 54-55 0.23901821296278314 1105 56-57 1.525679404646045 1105 58-59 -8.700070482508039 1105 60-61 -9.901429980729219 1105 62-63 -3.56202602956186 1105 64-65 -4.840388189817645 1105 66-67 -6.374943711539284 1105 68-69 -3.9774919435041944 1105 70-71 -3.911650047350223 1105 72-73 -2.0521622256593908 1105 74-75 -4.12154232259131 1105 76-77 -6.134097399401764 1105 78-79 -8.925824992274034 1105 80-81 -4.7484977972364995 1105 82-83 -2.3084831071742933 1105 84-85 -1.848712579524019 1105 86-87 -1.7110098748650024 1105 88-89 -1.7380536944277836 1105 90-91 -4.427494533467911 1105 92-93 -6.291521162463905 1105 94-95 -7.710516389102746 1105 96-97 -7.788081936372379 1105 98-99 -8.160720777634157 1105 100-101 -7.95684058584066 1106 1 0.20806339468886748 1106 2 0.22052011631903312 1106 3 0.13869765635015696 1106 4 0.07920544584501954 1106 5 -0.024039108092999584 1106 6 0.166023167243317 1106 7 0.14629105795287956 1106 8 0.22640666425601097 1106 9 0.2091988852804647 1106 10-11 0.13680966310130316 1106 12-13 0.2294135779791162 1106 14-15 0.35067419245343245 1106 16-17 0.394941275474622 1106 18-19 0.27653398554002706 1106 20-21 0.27535809976681236 1106 22-23 0.22591790912626664 1106 24-25 0.40213485012780836 1106 26-27 0.329570511653948 1106 28-29 0.40288429975188933 1106 30-31 0.3700469680970073 1106 32-33 0.37820690926226064 1106 34-35 0.37191765957453526 1106 36-37 0.2719219748526527 1106 38-39 0.20842178113631604 1106 40-41 0.09139167354742028 1106 42-43 0.044705135898304604 1106 44-45 0.043620176880004635 1106 46-47 0.06830352233581749 1106 48-49 0.10290794830255834 1106 50-51 0.05847843549946674 1106 52-53 0.20646496189311137 1106 54-55 0.1820316845088641 1106 56-57 1.4536842728683048 1106 58-59 -8.408090808734887 1106 60-61 -9.63975603970745 1106 62-63 -3.9065559243634915 1106 64-65 -5.680668882000511 1106 66-67 -6.719006718645769 1106 68-69 -4.051546976798356 1106 70-71 -4.297827373744939 1106 72-73 -4.682455368180793 1106 74-75 -5.49475870667726 1106 76-77 -6.406950415461303 1106 78-79 -9.482339387103647 1106 80-81 -5.500872454502463 1106 82-83 -3.056988017363988 1106 84-85 -2.531816490888801 1106 86-87 -2.2717437453814817 1106 88-89 -2.1667090578657557 1106 90-91 -4.688428798478267 1106 92-93 -6.951184513703343 1106 94-95 -7.511902013857936 1106 96-97 -7.059158331090078 1106 98-99 -6.567201598517153 1106 100-101 -7.030738241260055 1107 1 0.10449851422001188 1107 2 -7.542018095882952E-4 1107 3 -0.020533021092443704 1107 4 -0.02052326274130678 1107 5 0.024458032705467758 1107 6 -0.07725162818379516 1107 7 0.11255288141668984 1107 8 0.062388660006220675 1107 9 0.11380401256771222 1107 10-11 0.07114067648861067 1107 12-13 0.05476779426876277 1107 14-15 0.058375191338207344 1107 16-17 0.11804533342967716 1107 18-19 0.023931790884851978 1107 20-21 0.13075573186146272 1107 22-23 -0.030873555032748357 1107 24-25 -0.00744447894655309 1107 26-27 0.154081710958188 1107 28-29 0.058095802645517836 1107 30-31 0.09897211713804666 1107 32-33 0.10599904806433358 1107 34-35 -0.017013920375262614 1107 36-37 -0.0023017840565344727 1107 38-39 -0.06404366061720879 1107 40-41 -0.21447491005095287 1107 42-43 -0.233135937472575 1107 44-45 -0.14549343048691554 1107 46-47 -0.06319464993222113 1107 48-49 -0.12038499249866419 1107 50-51 -0.1478609432323026 1107 52-53 -0.17051450738767926 1107 54-55 -0.19688265073825306 1107 56-57 1.0289849938643876 1107 58-59 -9.130425423660558 1107 60-61 -10.641991655353664 1107 62-63 -4.3707308577023305 1107 64-65 -5.719097864345056 1107 66-67 -7.068421339583569 1107 68-69 -3.7109448808154664 1107 70-71 -4.181093472242827 1107 72-73 -2.7071973000005407 1107 74-75 -4.684087872827618 1107 76-77 -6.740158257154244 1107 78-79 -7.385185480140137 1107 80-81 -2.725517406382515 1107 82-83 -1.6773040956684788 1107 84-85 -1.4901383507007253 1107 86-87 -1.2929267342797033 1107 88-89 -1.238262526770221 1107 90-91 -0.9388161732447777 1107 92-93 -3.714645679275435 1107 94-95 -6.133619152545274 1107 96-97 -6.270414113744867 1107 98-99 -5.960754712889803 1107 100-101 -6.56260652064489 1108 1 -0.16275389177394928 1108 2 -0.14595455693936898 1108 3 -0.16791934468321656 1108 4 -0.08707740471650283 1108 5 -0.090756817464829 1108 6 -0.3131426458664066 1108 7 -0.2941093285377079 1108 8 -0.21777697112266736 1108 9 -0.13306549886112862 1108 10-11 -0.22853006394255715 1108 12-13 -0.16424503452424233 1108 14-15 -0.21920779476369745 1108 16-17 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11.664261758739947 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 9.0 4 45.0 5 90.0 6 239.0 7 559.0 8 1227.0 9 2124.0 10 3692.0 11 5693.0 12 8402.0 13 11555.0 14 14263.0 15 19099.0 16 25926.0 17 34204.0 18 35889.0 19 34419.0 20 35139.0 21 35214.0 22 34474.0 23 29969.0 24 30318.0 25 35057.0 26 48750.0 27 71984.0 28 102250.0 29 136438.0 30 164770.0 31 176058.0 32 170748.0 33 153396.0 34 134609.0 35 117873.0 36 92265.0 37 55910.0 38 19505.0 39 1096.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.97407379672528 20.25785117150321 14.271349857774334 22.496725173997177 2 15.369695088835442 22.267537722576904 39.44020135346072 22.922565835126935 3 18.632765008384634 27.60185389634521 31.010284535004146 22.755096560266008 4 12.406619149354023 17.426317965255002 38.1553206333568 32.01174225203417 5 12.219350170150072 39.59341086787401 34.529436179598505 13.657802782377408 6 29.68678285948033 37.88883596327807 18.142549767856696 14.281831409384903 7 26.37266534183784 34.50169997998109 22.077496518391087 17.04813815978998 8 25.69143756188203 34.97245317310692 20.648862727013796 18.68724653799726 9 26.864714543487672 15.890341992276719 20.90157753282503 36.343365931410574 10-11 24.24316075123504 27.03140309169959 28.17470478902031 20.550731368045057 12-13 25.787627125448527 24.707962748568026 28.59089720484056 20.91351292114289 14-15 22.773010974392722 25.522156435482955 27.485920893337322 24.218911696787 16-17 20.961064593236543 28.97589485573913 28.03899399866975 22.02404655235458 18-19 20.94870216003754 27.40969100856908 30.813994808097632 20.827612023295746 20-21 22.199306879449324 26.456253004191492 30.332324612181257 21.012115504177928 22-23 21.629829076220595 26.611197567772333 30.13988618463544 21.61908717137163 24-25 21.594752335267227 26.688721817564335 30.523941846447972 21.192584000720462 26-27 21.47746402691329 26.535234175666606 30.69860729916078 21.28869449825933 28-29 21.206022598851664 26.772645916782363 30.774591455795953 21.24674002857002 30-31 21.277881216123866 26.95172243158577 30.50054543644275 21.269850915847606 32-33 21.184327099452204 26.993712588982138 30.235942029300016 21.586018282265638 34-35 21.565346138759327 26.773798718191472 30.245386091514813 21.415469051534387 36-37 21.869849884441894 26.468033269220374 30.342101411435458 21.320015434902267 38-39 21.68809818763031 26.745609466401564 30.356662798004226 21.2096295479639 40-41 21.51655032558574 26.82211642002068 30.41386609337577 21.247467161017813 42-43 21.24743348795776 27.158178618374595 29.919392510673433 21.67499538299421 44-45 21.4189438933318 27.1737114243402 29.68061840008017 21.726726282247828 46-47 21.65664285700708 27.245635250281136 28.99305588557894 22.10466600713285 48-49 21.670070225558195 27.255283210497446 29.046768157896164 22.027878406048195 50-51 21.873912068228563 27.598911158507143 28.913119160471055 21.614057612793236 52-53 21.770983081586582 27.654350148577418 28.853992313884557 21.720674455951443 54-55 21.452355576934874 27.600804185761916 28.883119971463678 22.063720265839535 56-57 20.908276472391936 27.83067097183924 29.311683436688757 21.94936911908007 58-59 20.80334090500025 26.886749417516377 30.507974230422885 21.801935447060487 60-61 21.56073470400635 27.42143218468226 29.293097594663838 21.724735516647556 62-63 21.946712478559586 27.238626305108614 28.984350225519407 21.830310990812393 64-65 21.32933234367773 27.501779560461465 29.276967504044578 21.891920591816223 66-67 21.5663685759534 27.04490883755683 29.681019247355746 21.70770333913402 68-69 22.14157458582824 26.49140794518245 30.04930373452119 21.31771373446812 70-71 22.068540828791345 26.557562209016496 29.818391134290895 21.55550582790127 72-73 21.131041353868408 26.675110420281058 30.054599287147337 22.139248938703197 74-75 21.555402927741067 27.08665707160007 29.398913094331462 21.9590269063274 76-77 19.553620518002287 27.630228412581264 30.13881527931188 22.67733579010456 78-79 20.166466887779038 27.634198968817785 29.66011298803764 22.539221155365535 80-81 20.942235158189515 28.090964433719556 28.368860349906218 22.59794005818471 82-83 21.330778428559956 27.850397015709195 28.037376332698948 22.7814482230319 84-85 21.23834872369664 27.647452628586528 27.92869454166086 23.18550410605598 86-87 21.1268113769978 27.535371712558092 28.290154512787364 23.047662397656744 88-89 21.457811216181796 27.203077813641237 28.144482333305216 23.19462863687175 90-91 20.617776451861957 27.505824526402844 28.66132027674897 23.21507874498623 92-93 19.581539292856856 27.74871876299655 29.2079004326941 23.461841511452487 94-95 19.30327991380828 27.453346960424224 29.669733131215377 23.573639994552117 96-97 19.490607066981813 27.118351456436997 29.311249779681553 24.07979169689964 98-99 19.503183917507954 27.2556109029049 29.019267537967703 24.221937641619444 100-101 19.818862013058148 27.30385390648964 28.789053941545067 24.088230138907146 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1095.0 1 826.5 2 588.5 3 682.0 4 795.5 5 938.5 6 1229.0 7 1688.0 8 2218.5 9 2588.0 10 2789.0 11 2955.5 12 3123.0 13 3372.0 14 3680.0 15 3899.0 16 4125.0 17 4375.5 18 4562.0 19 4867.5 20 5318.5 21 5835.0 22 6518.5 23 7483.0 24 8774.0 25 10305.0 26 12380.0 27 14865.5 28 17672.5 29 20772.0 30 23762.0 31 27502.0 32 31727.5 33 35780.5 34 40242.5 35 44606.5 36 48599.5 37 52314.0 38 55740.5 39 58089.5 40 59622.5 41 60836.5 42 62045.0 43 64161.0 44 66211.0 45 68294.0 46 70639.0 47 72817.5 48 74894.0 49 73483.0 50 69313.0 51 65537.5 52 61579.0 53 57829.5 54 53916.0 55 49086.0 56 43930.0 57 38842.0 58 33865.0 59 29021.0 60 23749.0 61 18601.0 62 14012.5 63 10199.5 64 7245.5 65 5038.5 66 3468.0 67 2333.0 68 1566.5 69 1026.0 70 660.5 71 450.5 72 319.5 73 219.5 74 140.0 75 83.0 76 47.5 77 17.5 78 7.5 79 5.0 80 2.5 81 0.0 82 0.0 83 1.0 84 1.0 85 0.0 86 0.5 87 0.5 88 0.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02235172721344489 2 0.0071069812256341755 3 2.712588254058846E-4 4 0.0 5 0.1372569656553776 6 0.0 7 5.425176508117691E-5 8 0.0017903082476788384 9 0.0 10-11 0.0 12-13 0.0 14-15 6.781470635147115E-4 16-17 0.0 18-19 1.6275529524353074E-4 20-21 0.0 22-23 5.153917682711807E-4 24-25 0.0 26-27 2.712588254058846E-4 28-29 0.003634868260438853 30-31 0.012939045971860694 32-33 0.002766840019140023 34-35 0.00439439297157533 36-37 0.10722861368294619 38-39 0.106713221914675 40-41 0.11889274317539922 42-43 0.12141545025167394 44-45 0.11851298081983097 46-47 0.11167725841960269 48-49 0.11110761488625032 50-51 0.11040234194019502 52-53 0.08818624413945308 54-55 0.07568121228824179 56-57 0.5655203992061881 58-59 1.5873252686276147 60-61 0.5278968001223919 62-63 0.0037162459080606184 64-65 0.12008628200718509 66-67 1.9063798990700165 68-69 2.87688972460719 70-71 2.7817592545373464 72-73 2.6386702241357423 74-75 2.0139068974609087 76-77 1.423512063965001 78-79 0.9488362453872436 80-81 0.05541817803042222 82-83 3.255105904870615E-4 84-85 2.712588254058846E-4 86-87 6.781470635147115E-4 88-89 8.137764762176537E-5 90-91 0.2547934147037474 92-93 0.4033347474960098 94-95 2.010136399787767 96-97 2.4264101932556374 98-99 1.4403572370227067 100-101 0.9536375265969278 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1843258.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.76117455727264 #Duplication Level Percentage of deduplicated Percentage of total 1 70.88530172920149 32.437946659749294 2 14.411896947714675 13.19010663851592 3 5.565761624929475 7.640873677877014 4 2.763677571711996 5.058765271165281 5 1.5718316990907433 3.5964432378372977 6 1.0536038588370304 2.8928490059074434 7 0.6863094202034129 2.198442762476027 8 0.4868509976074933 1.7823098787919058 9 0.373013385102765 1.5362577565098112 >10 2.0049985619194954 16.237950076555677 >50 0.12705566571824697 4.007560767307837 >100 0.06376236150846903 5.111801948040915 >500 0.0037991529311204816 1.2306125527845566 >1k 0.0017808529364627256 1.6354743577093065 >5k 2.374470581950301E-4 0.8251531257645068 >10k+ 1.1872352909751505E-4 0.6174522830072176 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11365 0.6165713101475756 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8337 0.4522969654817719 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6851 0.37167884257114303 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 4349 0.23594092633803843 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 3914 0.21234140852772646 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 2397 0.13004148089958106 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1977 0.10725573956548676 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1977 0.10725573956548676 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 1947 0.10562818661305146 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1901 0.1031326054193173 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 1894 0.10275284306374907 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1847 0.10020301010493377 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 5.425176508117692E-5 7 0.0 0.0 0.0 0.0 5.425176508117692E-5 8 0.0 0.0 0.0 0.0 5.425176508117692E-5 9 0.0 0.0 0.0 0.0 1.0850353016235384E-4 10-11 0.0 0.0 0.0 2.712588254058846E-5 1.3562941270294228E-4 12-13 0.0 0.0 0.0 5.425176508117692E-5 1.6275529524353074E-4 14-15 0.0 0.0 0.0 8.137764762176538E-5 2.4413294286529613E-4 16-17 0.0 0.0 0.0 4.068882381088268E-4 2.7125882540588456E-4 18-19 0.0 0.0 0.0 5.696435333523576E-4 2.7125882540588456E-4 20-21 0.0 0.0 0.0 8.951541238394191E-4 2.7125882540588456E-4 22-23 0.0 0.0 0.0 0.002305700015950019 3.5263647302764996E-4 24-25 0.0 0.0 0.0 0.0037704976731417956 4.8826588573059227E-4 26-27 0.0 0.0 0.0 0.004909784739846511 4.8826588573059227E-4 28-29 0.0 0.0 0.0 0.007568121228824181 4.8826588573059227E-4 30-31 0.0 0.0 0.0 0.017360564825976615 4.8826588573059227E-4 32-33 0.0 0.0 0.0 0.03705395555044383 5.425176508117691E-4 34-35 0.0 0.0 0.0 0.06653978987206349 5.425176508117691E-4 36-37 0.0 0.0 0.0 0.11354894431490328 5.425176508117691E-4 38-39 0.0 0.0 0.0 0.17965472006631736 5.425176508117691E-4 40-41 0.0 0.0 0.0 0.2635008229992763 5.425176508117691E-4 42-43 0.0 0.0 0.0 0.3443630788527705 5.425176508117691E-4 44-45 0.0 0.0 0.0 0.4412024795226713 5.425176508117691E-4 46-47 0.0 0.0 0.0 0.5495703802723222 5.425176508117691E-4 48-49 0.0 2.712588254058846E-5 0.0 0.6522960974535306 5.967694158929461E-4 50-51 0.0 5.425176508117692E-5 0.0 0.7535298910950068 5.967694158929461E-4 52-53 0.0 5.425176508117692E-5 0.0 0.8541397894380494 5.967694158929461E-4 54-55 0.0 5.425176508117692E-5 0.0 0.9564857442636896 5.967694158929461E-4 56-57 0.0 5.425176508117692E-5 0.0 1.0417152672062184 5.967694158929461E-4 58-59 5.425176508117692E-5 5.425176508117692E-5 0.0 1.1000359146684837 5.967694158929461E-4 60-61 5.425176508117692E-5 5.425176508117692E-5 0.0 1.152551623267063 5.967694158929461E-4 62-63 1.0850353016235382E-4 5.425176508117692E-5 0.0 1.197634840049521 5.967694158929461E-4 64-65 1.6275529524353074E-4 5.425176508117692E-5 0.0 1.236723236790509 5.967694158929461E-4 66-67 1.6275529524353074E-4 5.425176508117692E-5 0.0 1.2733974299853847 6.238952984335346E-4 68-69 1.6275529524353074E-4 5.425176508117692E-5 0.0 1.3245297185743938 6.51021180974123E-4 70-71 2.1700706032470768E-4 5.425176508117692E-5 0.0 1.4049037085421574 6.51021180974123E-4 72-73 2.1700706032470768E-4 5.425176508117692E-5 0.0 1.5207854787555513 6.51021180974123E-4 74-75 2.1700706032470768E-4 5.425176508117692E-5 0.0 1.6652579291667253 6.51021180974123E-4 76-77 2.1700706032470768E-4 5.425176508117692E-5 0.0 1.889127837774202 6.51021180974123E-4 78-79 2.1700706032470768E-4 5.425176508117692E-5 0.0 2.2386990860747655 6.51021180974123E-4 80-81 2.1700706032470768E-4 5.425176508117692E-5 0.0 2.678382516175164 6.781470635147114E-4 82-83 2.1700706032470768E-4 5.425176508117692E-5 0.0 3.1218635698312447 7.052729460552999E-4 84-85 2.1700706032470768E-4 5.425176508117692E-5 0.0 3.4563528274392405 7.052729460552999E-4 86-87 2.1700706032470768E-4 5.425176508117692E-5 0.0 3.7157305162923473 7.052729460552999E-4 88-89 2.1700706032470768E-4 5.425176508117692E-5 0.0 3.9520512049859544 7.052729460552999E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 10845 0.0 28.547285 1 TACATGG 10890 0.0 28.123062 2 ACATGGG 10830 0.0 26.975695 3 CATGGGA 7335 0.0 24.17969 4 GTATCAA 16505 0.0 24.03148 1 CATGGGG 3840 0.0 23.399773 4 GGTATCA 12200 0.0 22.561321 1 ATGGGAG 2680 0.0 21.942429 5 ATGGGAT 2940 0.0 21.762114 5 GAGTACT 8580 0.0 21.214542 12-13 AGTACTT 8955 0.0 21.191664 12-13 GTCGGGA 455 0.0 20.67893 2 TCAACGC 19280 0.0 20.496595 4 ATCAACG 19320 0.0 20.45416 3 TATCAAC 19635 0.0 20.245815 2 CAACGCA 19570 0.0 20.216904 5 AACGCAG 19565 0.0 20.193544 6 ATGGGGA 1940 0.0 19.157324 5 GTACTTT 9565 0.0 19.128096 14-15 CGCAGAG 21160 0.0 18.360573 8 >>END_MODULE