FastQCFastQC Report
Fri 27 May 2016
SRR939636_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939636_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1761818
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43210.24525802324644203No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28100.1594943405050919No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22200.12600620495420073No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA18660.10591332362366601No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG83750.035.931841
GTATCAA133150.029.342691
TACATGG101750.028.188572
ACATGGG99250.028.041653
GGTATCA101500.025.5275971
CATGGGA65700.024.7378774
CATGGGG37000.023.4955434
TATCAAC170900.023.3356112
GAGTACT76850.023.24922812-13
AGTACTT81300.022.81857512-13
GTACTAG2702.3449228E-422.6688651
GTACTAA3703.534069E-622.0561921
ATCAACG184100.021.5854453
TCAACGC184550.021.5559644
ATGGGAG28050.021.3738165
CAACGCA186050.021.339175
AACGCAG188600.021.2264656
GTACTTT83950.021.08823814-15
ATGGGAT26200.020.5406785
ACGCAGA193900.020.5239527
Produced by FastQC (version 0.11.5)