FastQCFastQC Report
Fri 27 May 2016
SRR939636_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939636_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1761818
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT108330.6148762244454308No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78470.4453922028268527No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63900.36269353588168585No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA41500.23555213989186172No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA35990.20427762686043618No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT22940.1302064117860074No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19690.11175955745712668No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA19230.10914861807519277No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA18550.10528896855407312No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT17760.10080496396336057No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG101000.026.5811541
TACATGG100900.026.0404382
ACATGGG99850.024.7531493
GTATCAA148850.024.3124921
GGTATCA110400.023.7195721
CATGGGA66450.023.6372284
ATGGGAG26400.021.0826575
AGTACTT86900.020.58581412-13
GAGTACT83450.020.50483712-13
TCAACGC177300.020.3250624
ATCAACG178250.020.2167383
TATCAAC179300.020.1526762
CAACGCA179100.020.1471255
AACGCAG181250.019.9211526
ATGGGAT27400.019.4524865
GTACTTT91050.018.71562614-15
ACGCAGA196300.018.321797
CATGGGG37100.018.3077874
CGCAGAG196450.018.2118238
AGTACAT74800.017.9732882
Produced by FastQC (version 0.11.5)